AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64433

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64433.t1 MTR_2g041620 94.352 301 14 1 4 304 1 298 0.0 590
MS.gene64433.t1 MTR_2g041620 90.033 301 15 2 4 304 1 286 0.0 556
MS.gene64433.t1 MTR_2g041620 93.640 283 15 1 4 286 1 280 0.0 552
MS.gene64433.t1 MTR_2g041620 93.750 256 13 1 4 259 1 253 2.42e-180 498
MS.gene64433.t1 MTR_4g129490 27.979 193 104 5 112 283 58 236 1.99e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64433.t1 AT2G06025 56.187 299 117 3 4 302 1 285 1.23e-116 338
MS.gene64433.t1 AT2G06025 56.187 299 117 3 4 302 1 285 1.23e-116 338
MS.gene64433.t1 AT2G06025 56.187 299 117 3 4 302 1 285 1.23e-116 338
MS.gene64433.t1 AT2G06025 56.250 256 98 3 4 259 1 242 1.35e-98 290
MS.gene64433.t1 AT2G06025 56.250 256 98 3 4 259 1 242 1.35e-98 290
MS.gene64433.t1 AT2G06025 56.250 256 98 3 4 259 1 242 1.35e-98 290
MS.gene64433.t1 AT2G06025 53.604 222 89 3 4 225 1 208 1.71e-78 238
MS.gene64433.t1 AT4G28030 30.583 206 101 7 108 289 58 245 3.19e-13 68.9

Find 59 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTTCTTCCACCAATTT+AGG 0.169798 2.4:-48542251 None:intergenic
AAGTCCAATTGTTCCTAAAT+TGG 0.216411 2.4:+48542238 MS.gene64433:CDS
AGTCCATGAGGATGTAATTT+TGG 0.254723 2.4:-48541980 None:intergenic
TCCTTGTCGACGAGCCAATT+TGG 0.274791 2.4:-48544392 None:intergenic
AAATTTATGTGTTGCCAAAT+TGG 0.283413 2.4:+48544378 MS.gene64433:CDS
TGAAATTCAGGTTTATGAAT+TGG 0.285497 2.4:-48541913 None:intergenic
GTTTGATATGAATAGGTCTA+TGG 0.288131 2.4:+48542133 MS.gene64433:intron
TAAAGTAAGATATGAAATTC+AGG 0.293568 2.4:-48541925 None:intergenic
AGAGAAAATAGATTTGAGTT+TGG 0.303539 2.4:+48542329 MS.gene64433:CDS
AAGCGGTGCAAGGTGCAAAA+TGG 0.311231 2.4:+48543891 MS.gene64433:CDS
GAGAAAATAGATTTGAGTTT+GGG 0.321359 2.4:+48542330 MS.gene64433:CDS
CAAAAGGAAATTTGCTGATC+AGG 0.341147 2.4:+48543761 MS.gene64433:CDS
CTGTGCTTGATGAAGAGTTT+TGG 0.345709 2.4:+48542372 MS.gene64433:CDS
CTGTTTATGCTGCAACAAAA+AGG 0.359050 2.4:-48544325 None:intergenic
TCCACCAATTTAGGAACAAT+TGG 0.361687 2.4:-48542242 None:intergenic
TTTGGTGCATTTGTAATTAT+GGG 0.370191 2.4:-48541962 None:intergenic
TTACCTCAAAGCCCATCTTT+TGG 0.386865 2.4:-48544587 None:intergenic
TGCAGACAGCAGCCTGGCTA+AGG 0.397087 2.4:+48543173 MS.gene64433:intron
TCTCTCCTTGCAGCAAATAT+CGG 0.397446 2.4:-48544188 None:intergenic
GGCTAAGGGCAGAAAGTCAT+TGG 0.397544 2.4:+48543188 MS.gene64433:CDS
AGCGGTGCAAGGTGCAAAAT+GGG 0.411100 2.4:+48543892 MS.gene64433:CDS
TCATTTAGCAGGTGTGAGTC+AGG 0.439184 2.4:+48544518 MS.gene64433:intron
ATGACTTTCTGCCCTTAGCC+AGG 0.443627 2.4:-48543185 None:intergenic
AGAACACCACCAAGTAGATA+TGG 0.454568 2.4:+48544346 MS.gene64433:CDS
GTTCCTAAATTGGTGGAAGA+AGG 0.454847 2.4:+48542248 MS.gene64433:CDS
GTTGAAAATGCAAAATCTAA+TGG 0.457985 2.4:+48544442 MS.gene64433:CDS
GAAGAAGGTTTGAGCTCAAA+TGG 0.457998 2.4:+48542263 MS.gene64433:CDS
AGTTTGGGCAATTTGTAGCT+AGG 0.464831 2.4:+48542345 MS.gene64433:CDS
ACAGTTATTGTACCAAAAGA+TGG 0.467482 2.4:+48544575 MS.gene64433:CDS
GTACCAAAAGATGGGCTTTG+AGG 0.468548 2.4:+48544584 MS.gene64433:CDS
ATTTGATCGGTTGCAGCCAT+CGG 0.477407 2.4:+48542292 MS.gene64433:CDS
TCATGTTTATCAGGTGAGAA+AGG 0.487782 2.4:+48544104 MS.gene64433:intron
TGAATTGGAATAGTTGACAT+AGG 0.492624 2.4:-48541898 None:intergenic
CGTAGCAAGTATGTTTCCTC+TGG 0.493901 2.4:-48544734 None:intergenic
GATATATTGATATCTACAAA+AGG 0.499748 2.4:+48543745 MS.gene64433:intron
CTAATCAACTCTTGTTCCGA+TGG 0.513135 2.4:-48542308 None:intergenic
TGCATGTGGACAGAAATAAT+AGG 0.521792 2.4:+48544547 MS.gene64433:CDS
GCCAAATTGGCTCGTCGACA+AGG 0.522479 2.4:+48544391 MS.gene64433:CDS
TCCAATTGTTCCTAAATTGG+TGG 0.526623 2.4:+48542241 MS.gene64433:CDS
GCACCAAAATTACATCCTCA+TGG 0.535293 2.4:+48541977 MS.gene64433:CDS
GCAATGTAACCATATCTACT+TGG 0.536879 2.4:-48544355 None:intergenic
CAAATGGTCTTCGATTTGAT+CGG 0.545639 2.4:+48542279 MS.gene64433:CDS
GTGCATTTGTAATTATGGGT+TGG 0.548458 2.4:-48541958 None:intergenic
GTTTGGGCAATTTGTAGCTA+GGG 0.550313 2.4:+48542346 MS.gene64433:CDS
GCTAAGGGCAGAAAGTCATT+GGG 0.553474 2.4:+48543189 MS.gene64433:CDS
CAGTTATTGTACCAAAAGAT+GGG 0.555181 2.4:+48544576 MS.gene64433:CDS
GTGGACAGAAATAATAGGCC+GGG 0.563824 2.4:+48544552 MS.gene64433:CDS
TATTAAGAATAAGAAGATGA+AGG 0.572830 2.4:+48542193 MS.gene64433:CDS
TGGTTGAAACTGCAAACTCT+CGG 0.577388 2.4:+48544705 MS.gene64433:CDS
GCAGACAGCAGCCTGGCTAA+GGG 0.579446 2.4:+48543174 MS.gene64433:intron
TGTGGACAGAAATAATAGGC+CGG 0.580627 2.4:+48544551 MS.gene64433:CDS
GGGAGAATCAAACATATGAA+AGG 0.580964 2.4:+48543209 MS.gene64433:CDS
AATATCCGATATTTGCTGCA+AGG 0.586800 2.4:+48544183 MS.gene64433:CDS
CAGCTGCGCATGCATTATCG+CGG 0.671835 2.4:+48543917 MS.gene64433:CDS
AAACTCTCGGTTGTTACCAG+AGG 0.674396 2.4:+48544718 MS.gene64433:CDS
ATGTAACCATATCTACTTGG+TGG 0.675557 2.4:-48544352 None:intergenic
GAGTCAGGTATATGTGCATG+TGG 0.676318 2.4:+48544533 MS.gene64433:CDS
GCGGTGCAAGGTGCAAAATG+GGG 0.677118 2.4:+48543893 MS.gene64433:CDS
TATTGTTTAGGAATTTAATG+CGG 0.686710 2.4:+48543863 MS.gene64433:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAACAAATTCAAA+CGG - chr2.4:48542429-48542448 None:intergenic 10.0%
!! AAAAACATTTAATGAAATGA+AGG - chr2.4:48543329-48543348 None:intergenic 15.0%
!! AAAAATAAACAAATTCAAAC+GGG - chr2.4:48542428-48542447 None:intergenic 15.0%
!! AAAACATTTAATGAAATGAA+GGG - chr2.4:48543328-48543347 None:intergenic 15.0%
!! AATTATTGTTTGATATGAAT+AGG + chr2.4:48542126-48542145 MS.gene64433:intron 15.0%
!! ATATTTAACCCTAAAATAAT+TGG - chr2.4:48543438-48543457 None:intergenic 15.0%
!! TATTTAACCCTAAAATAATT+GGG - chr2.4:48543437-48543456 None:intergenic 15.0%
!!! AAATATGTTTTTCATACATA+AGG + chr2.4:48543452-48543471 MS.gene64433:intron 15.0%
!!! TTTTCTAAATAGTAGAATAT+AGG + chr2.4:48543617-48543636 MS.gene64433:intron 15.0%
!! ACAAATGAATGATAAATGAT+TGG - chr2.4:48543083-48543102 None:intergenic 20.0%
!! ATAAATCAGTCAATTCAAAT+GGG + chr2.4:48542619-48542638 MS.gene64433:intron 20.0%
!! CATTAAATTCCTAAACAATA+GGG - chr2.4:48543863-48543882 None:intergenic 20.0%
!! GATATATTGATATCTACAAA+AGG + chr2.4:48543745-48543764 MS.gene64433:intron 20.0%
!! TAAAGTAAGATATGAAATTC+AGG - chr2.4:48541928-48541947 None:intergenic 20.0%
!! TACAATTTCATGAACTAAAA+TGG + chr2.4:48542901-48542920 MS.gene64433:intron 20.0%
!! TATTAAGAATAAGAAGATGA+AGG + chr2.4:48542193-48542212 MS.gene64433:CDS 20.0%
!! TATTGTTTAGGAATTTAATG+CGG + chr2.4:48543863-48543882 MS.gene64433:intron 20.0%
!! TGATCTAGTTAAAAAAATTC+AGG + chr2.4:48543963-48543982 MS.gene64433:intron 20.0%
!! TTCATGAAATTGTAGAAATT+TGG - chr2.4:48542895-48542914 None:intergenic 20.0%
!!! TATAATTTGTTTGTGTACTA+TGG + chr2.4:48542074-48542093 MS.gene64433:intron 20.0%
!!! TTTGAAATTGAAAATTCTCA+AGG + chr2.4:48542704-48542723 MS.gene64433:intron 20.0%
! AAATTTATGTGTTGCCAAAT+TGG + chr2.4:48544378-48544397 MS.gene64433:CDS 25.0%
! AATAAAGCTTAACCTAATGT+TGG + chr2.4:48543023-48543042 MS.gene64433:intron 25.0%
! AATTTAATGCGGTAAAAAAG+CGG + chr2.4:48543874-48543893 MS.gene64433:CDS 25.0%
! ACATGTGAAATCAATAACTA+TGG + chr2.4:48544660-48544679 MS.gene64433:intron 25.0%
! CAACAATCTATAACAAAAAC+TGG - chr2.4:48544638-48544657 None:intergenic 25.0%
! CATAAATCAGTCAATTCAAA+TGG + chr2.4:48542618-48542637 MS.gene64433:intron 25.0%
! CATACAATTTCATACACTAA+GGG - chr2.4:48542645-48542664 None:intergenic 25.0%
! GCATTAAATTCCTAAACAAT+AGG - chr2.4:48543864-48543883 None:intergenic 25.0%
! GTTGAAAATGCAAAATCTAA+TGG + chr2.4:48544442-48544461 MS.gene64433:CDS 25.0%
!! AGAGAAAATAGATTTGAGTT+TGG + chr2.4:48542329-48542348 MS.gene64433:CDS 25.0%
!! ATCATTCATTTGTACTTTTG+TGG + chr2.4:48543090-48543109 MS.gene64433:intron 25.0%
!! GAGAAAATAGATTTGAGTTT+GGG + chr2.4:48542330-48542349 MS.gene64433:CDS 25.0%
!! TGAAATTCAGGTTTATGAAT+TGG - chr2.4:48541916-48541935 None:intergenic 25.0%
!! TTTGGTGCATTTGTAATTAT+GGG - chr2.4:48541965-48541984 None:intergenic 25.0%
!! TTTTCTTTGATTCCAACATT+AGG - chr2.4:48543038-48543057 None:intergenic 25.0%
!! TTTTTGAATTCTGTGTTCTA+TGG + chr2.4:48542098-48542117 MS.gene64433:intron 25.0%
!!! CATTTGACTTGATTAACAAT+AGG + chr2.4:48543678-48543697 MS.gene64433:intron 25.0%
!!! GTTAAGTTGAAGTAACTTTA+TGG + chr2.4:48544475-48544494 MS.gene64433:intron 25.0%
!!! TCGATAATAAAAAGTGTTGT+AGG + chr2.4:48544147-48544166 MS.gene64433:CDS 25.0%
!!! TTTTGGTGCATTTGTAATTA+TGG - chr2.4:48541966-48541985 None:intergenic 25.0%
AAAAACTTACATAGTCCATG+AGG - chr2.4:48541995-48542014 None:intergenic 30.0%
AACAAGAATCCCTATTGTTT+AGG + chr2.4:48543851-48543870 MS.gene64433:intron 30.0%
AAGTCCAATTGTTCCTAAAT+TGG + chr2.4:48542238-48542257 MS.gene64433:CDS 30.0%
ACAGTTATTGTACCAAAAGA+TGG + chr2.4:48544575-48544594 MS.gene64433:CDS 30.0%
ATGAAGGGACTATAATTCAA+AGG - chr2.4:48543313-48543332 None:intergenic 30.0%
CAGTTATTGTACCAAAAGAT+GGG + chr2.4:48544576-48544595 MS.gene64433:CDS 30.0%
CCATACAATTTCATACACTA+AGG - chr2.4:48542646-48542665 None:intergenic 30.0%
CCTTAGTGTATGAAATTGTA+TGG + chr2.4:48542643-48542662 MS.gene64433:intron 30.0%
GATTATAAACCAGATCAAGA+TGG + chr2.4:48543577-48543596 MS.gene64433:intron 30.0%
GTTTGATATGAATAGGTCTA+TGG + chr2.4:48542133-48542152 MS.gene64433:intron 30.0%
TGAAGGGACTATAATTCAAA+GGG - chr2.4:48543312-48543331 None:intergenic 30.0%
TGAATTGGAATAGTTGACAT+AGG - chr2.4:48541901-48541920 None:intergenic 30.0%
TGTTTGGATTCATGTTTATC+AGG + chr2.4:48544095-48544114 MS.gene64433:intron 30.0%
TTAACCCTATGACAAAACAT+TGG - chr2.4:48543261-48543280 None:intergenic 30.0%
! AATCTCCAATGTTTTGTCAT+AGG + chr2.4:48543253-48543272 MS.gene64433:intron 30.0%
! ATCTCCAATGTTTTGTCATA+GGG + chr2.4:48543254-48543273 MS.gene64433:intron 30.0%
! ATTCACTTTGTCTGTCTTTT+AGG + chr2.4:48542926-48542945 MS.gene64433:intron 30.0%
! TTTCAACCATCTGAAATTGA+CGG - chr2.4:48544694-48544713 None:intergenic 30.0%
!! ACTTGTAGGATTAGTTGATT+TGG + chr2.4:48542761-48542780 MS.gene64433:intron 30.0%
!! CTTTGAGGTAATGTAATTGT+TGG + chr2.4:48544599-48544618 MS.gene64433:intron 30.0%
!! TTGGGTATGTTTTAAACTGT+TGG + chr2.4:48542391-48542410 MS.gene64433:intron 30.0%
AAAATGTGTTCCTTTCATCG+TGG + chr2.4:48543495-48543514 MS.gene64433:intron 35.0%
AAACCTTCTTCCACCAATTT+AGG - chr2.4:48542254-48542273 None:intergenic 35.0%
AAATGATTTACACCCACAAC+CGG - chr2.4:48543663-48543682 None:intergenic 35.0%
AACTTGTGTTGAGCATATTG+TGG + chr2.4:48543537-48543556 MS.gene64433:intron 35.0%
AGTCATCATATCATTTCTGC+AGG + chr2.4:48544288-48544307 MS.gene64433:intron 35.0%
ATCAACTAATCCTACAAGTC+AGG - chr2.4:48542760-48542779 None:intergenic 35.0%
ATGTAACCATATCTACTTGG+TGG - chr2.4:48544355-48544374 None:intergenic 35.0%
CCAGTGTATAGTACTAAGTA+AGG - chr2.4:48543810-48543829 None:intergenic 35.0%
CCTTACTTAGTACTATACAC+TGG + chr2.4:48543807-48543826 MS.gene64433:intron 35.0%
CTGTTTATGCTGCAACAAAA+AGG - chr2.4:48544328-48544347 None:intergenic 35.0%
CTTGACTATGATTCGACTTA+CGG + chr2.4:48544072-48544091 MS.gene64433:intron 35.0%
GAAGGGACTATAATTCAAAG+GGG - chr2.4:48543311-48543330 None:intergenic 35.0%
GATCACAGAGATAAAATGTG+AGG - chr2.4:48543357-48543376 None:intergenic 35.0%
GATTTGTGGATTCATTTAGC+AGG + chr2.4:48544507-48544526 MS.gene64433:intron 35.0%
GCAATGTAACCATATCTACT+TGG - chr2.4:48544358-48544377 None:intergenic 35.0%
GCTAAATGAATCCACAAATC+AGG - chr2.4:48544507-48544526 None:intergenic 35.0%
GGGAGAATCAAACATATGAA+AGG + chr2.4:48543209-48543228 MS.gene64433:CDS 35.0%
GTGCATTTGTAATTATGGGT+TGG - chr2.4:48541961-48541980 None:intergenic 35.0%
TCATGTTTATCAGGTGAGAA+AGG + chr2.4:48544104-48544123 MS.gene64433:intron 35.0%
TCCAATTGTTCCTAAATTGG+TGG + chr2.4:48542241-48542260 MS.gene64433:CDS 35.0%
TCCACCAATTTAGGAACAAT+TGG - chr2.4:48542245-48542264 None:intergenic 35.0%
TGCATGTGGACAGAAATAAT+AGG + chr2.4:48544547-48544566 MS.gene64433:CDS 35.0%
! AATATCCGATATTTGCTGCA+AGG + chr2.4:48544183-48544202 MS.gene64433:CDS 35.0%
! AGTCCATGAGGATGTAATTT+TGG - chr2.4:48541983-48542002 None:intergenic 35.0%
! CAAATGGTCTTCGATTTGAT+CGG + chr2.4:48542279-48542298 MS.gene64433:CDS 35.0%
! CAATTTATTGCCTGACTTGT+AGG + chr2.4:48542747-48542766 MS.gene64433:intron 35.0%
! TGTGCTTGATGAAGAGTTTT+GGG + chr2.4:48542373-48542392 MS.gene64433:CDS 35.0%
! TTTTGGTACAATAACTGTCC+CGG - chr2.4:48544573-48544592 None:intergenic 35.0%
!! AGTGGGTCCCAATTATTTTA+GGG + chr2.4:48543427-48543446 MS.gene64433:intron 35.0%
!! CAAAAGGAAATTTGCTGATC+AGG + chr2.4:48543761-48543780 MS.gene64433:CDS 35.0%
!! TAGTGGGTCCCAATTATTTT+AGG + chr2.4:48543426-48543445 MS.gene64433:intron 35.0%
AACACAACAACCACGATGAA+AGG - chr2.4:48543508-48543527 None:intergenic 40.0%
AATGCTGATATGGCAGTTAG+TGG + chr2.4:48543409-48543428 MS.gene64433:intron 40.0%
AGAACACCACCAAGTAGATA+TGG + chr2.4:48544346-48544365 MS.gene64433:CDS 40.0%
AGTAATCTGAATGACCCTAC+CGG + chr2.4:48543641-48543660 MS.gene64433:intron 40.0%
AGTTTGGGCAATTTGTAGCT+AGG + chr2.4:48542345-48542364 MS.gene64433:CDS 40.0%
ATGATTCGACTTACGGTGTT+TGG + chr2.4:48544079-48544098 MS.gene64433:intron 40.0%
ATGCTGATATGGCAGTTAGT+GGG + chr2.4:48543410-48543429 MS.gene64433:intron 40.0%
ATTTCGCCGTCAATTTCAGA+TGG + chr2.4:48544685-48544704 MS.gene64433:intron 40.0%
CCCTGCTGTTAATTCTCTAA+CGG + chr2.4:48543386-48543405 MS.gene64433:intron 40.0%
CCGTTAGAGAATTAACAGCA+GGG - chr2.4:48543389-48543408 None:intergenic 40.0%
CGTAAGTCGAATCATAGTCA+AGG - chr2.4:48544074-48544093 None:intergenic 40.0%
CTAATCAACTCTTGTTCCGA+TGG - chr2.4:48542311-48542330 None:intergenic 40.0%
GAAGAAGGTTTGAGCTCAAA+TGG + chr2.4:48542263-48542282 MS.gene64433:CDS 40.0%
GCACCAAAATTACATCCTCA+TGG + chr2.4:48541977-48541996 MS.gene64433:CDS 40.0%
GTTCCTAAATTGGTGGAAGA+AGG + chr2.4:48542248-48542267 MS.gene64433:CDS 40.0%
GTTTGGGCAATTTGTAGCTA+GGG + chr2.4:48542346-48542365 MS.gene64433:CDS 40.0%
TACTGTTCTGTCTAGACTGA+TGG + chr2.4:48544244-48544263 MS.gene64433:intron 40.0%
TCCGTTAGAGAATTAACAGC+AGG - chr2.4:48543390-48543409 None:intergenic 40.0%
TCTCTAACGGAATGCTGATA+TGG + chr2.4:48543399-48543418 MS.gene64433:intron 40.0%
TCTCTCCTTGCAGCAAATAT+CGG - chr2.4:48544191-48544210 None:intergenic 40.0%
TGACATCATCCATCTTGATC+TGG - chr2.4:48543589-48543608 None:intergenic 40.0%
TGGTTGAAACTGCAAACTCT+CGG + chr2.4:48544705-48544724 MS.gene64433:CDS 40.0%
TGTGGACAGAAATAATAGGC+CGG + chr2.4:48544551-48544570 MS.gene64433:CDS 40.0%
TTTGCTGATCAGGTAGGAAT+CGG + chr2.4:48543771-48543790 MS.gene64433:intron 40.0%
! CTGTGCTTGATGAAGAGTTT+TGG + chr2.4:48542372-48542391 MS.gene64433:CDS 40.0%
! TATGGTTTACGCCTGATTTG+TGG + chr2.4:48544493-48544512 MS.gene64433:intron 40.0%
! TTACCTCAAAGCCCATCTTT+TGG - chr2.4:48544590-48544609 None:intergenic 40.0%
!! AGGAAATTTGCTGATCAGGT+AGG + chr2.4:48543765-48543784 MS.gene64433:intron 40.0%
AAACTCTCGGTTGTTACCAG+AGG + chr2.4:48544718-48544737 MS.gene64433:CDS 45.0%
ATTTACACCCACAACCGGTA+GGG - chr2.4:48543658-48543677 None:intergenic 45.0%
ATTTGATCGGTTGCAGCCAT+CGG + chr2.4:48542292-48542311 MS.gene64433:CDS 45.0%
CGTAGCAAGTATGTTTCCTC+TGG - chr2.4:48544737-48544756 None:intergenic 45.0%
GAAAGAATGAGTAGTACCCC+AGG - chr2.4:48544219-48544238 None:intergenic 45.0%
GAGTCAGGTATATGTGCATG+TGG + chr2.4:48544533-48544552 MS.gene64433:CDS 45.0%
GCTAAGGGCAGAAAGTCATT+GGG + chr2.4:48543189-48543208 MS.gene64433:CDS 45.0%
GTGGACAGAAATAATAGGCC+GGG + chr2.4:48544552-48544571 MS.gene64433:CDS 45.0%
TCATTTAGCAGGTGTGAGTC+AGG + chr2.4:48544518-48544537 MS.gene64433:intron 45.0%
! GTACCAAAAGATGGGCTTTG+AGG + chr2.4:48544584-48544603 MS.gene64433:CDS 45.0%
! TCAAAGGGGCGCAAAATGTA+AGG - chr2.4:48543297-48543316 None:intergenic 45.0%
! TGCAAGGAGAGACTTTTCCT+GGG + chr2.4:48544199-48544218 MS.gene64433:CDS 45.0%
AAGCGGTGCAAGGTGCAAAA+TGG + chr2.4:48543891-48543910 MS.gene64433:CDS 50.0%
AGCGGTGCAAGGTGCAAAAT+GGG + chr2.4:48543892-48543911 MS.gene64433:CDS 50.0%
ATGACTTTCTGCCCTTAGCC+AGG - chr2.4:48543188-48543207 None:intergenic 50.0%
GATTTACACCCACAACCGGT+AGG - chr2.4:48543659-48543678 None:intergenic 50.0%
GGCTAAGGGCAGAAAGTCAT+TGG + chr2.4:48543188-48543207 MS.gene64433:CDS 50.0%
TCCTTGTCGACGAGCCAATT+TGG - chr2.4:48544395-48544414 None:intergenic 50.0%
TGCGGTAAAAAAGCGGTGCA+AGG + chr2.4:48543881-48543900 MS.gene64433:CDS 50.0%
! CTGCAAGGAGAGACTTTTCC+TGG + chr2.4:48544198-48544217 MS.gene64433:CDS 50.0%
! GCAAGGAGAGACTTTTCCTG+GGG + chr2.4:48544200-48544219 MS.gene64433:CDS 50.0%
! TGAATGACCCTACCGGTTGT+GGG + chr2.4:48543648-48543667 MS.gene64433:intron 50.0%
! TTCTTTTGCAGACAGCAGCC+TGG + chr2.4:48543167-48543186 MS.gene64433:intron 50.0%
CAGCTGCGCATGCATTATCG+CGG + chr2.4:48543917-48543936 MS.gene64433:CDS 55.0%
GCGGTGCAAGGTGCAAAATG+GGG + chr2.4:48543893-48543912 MS.gene64433:CDS 55.0%
GCTGATCAGGTAGGAATCGG+CGG + chr2.4:48543774-48543793 MS.gene64433:intron 55.0%
! CTGAATGACCCTACCGGTTG+TGG + chr2.4:48543647-48543666 MS.gene64433:intron 55.0%
!! GCCAAATTGGCTCGTCGACA+AGG + chr2.4:48544391-48544410 MS.gene64433:CDS 55.0%
GCAGACAGCAGCCTGGCTAA+GGG + chr2.4:48543174-48543193 MS.gene64433:intron 60.0%
TGCAGACAGCAGCCTGGCTA+AGG + chr2.4:48543173-48543192 MS.gene64433:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 48541892 48544769 48541892 ID=MS.gene64433
chr2.4 mRNA 48541892 48544769 48541892 ID=MS.gene64433.t1;Parent=MS.gene64433
chr2.4 exon 48541892 48542004 48541892 ID=MS.gene64433.t1.exon1;Parent=MS.gene64433.t1
chr2.4 CDS 48541892 48542004 48541892 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48542148 48542394 48542148 ID=MS.gene64433.t1.exon2;Parent=MS.gene64433.t1
chr2.4 CDS 48542148 48542394 48542148 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48543178 48543230 48543178 ID=MS.gene64433.t1.exon3;Parent=MS.gene64433.t1
chr2.4 CDS 48543178 48543230 48543178 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48543746 48543782 48543746 ID=MS.gene64433.t1.exon4;Parent=MS.gene64433.t1
chr2.4 CDS 48543746 48543782 48543746 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48543873 48543938 48543873 ID=MS.gene64433.t1.exon5;Parent=MS.gene64433.t1
chr2.4 CDS 48543873 48543938 48543873 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48544117 48544221 48544117 ID=MS.gene64433.t1.exon6;Parent=MS.gene64433.t1
chr2.4 CDS 48544117 48544221 48544117 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48544310 48544463 48544310 ID=MS.gene64433.t1.exon7;Parent=MS.gene64433.t1
chr2.4 CDS 48544310 48544463 48544310 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48544529 48544605 48544529 ID=MS.gene64433.t1.exon8;Parent=MS.gene64433.t1
chr2.4 CDS 48544529 48544605 48544529 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
chr2.4 exon 48544704 48544769 48544704 ID=MS.gene64433.t1.exon9;Parent=MS.gene64433.t1
chr2.4 CDS 48544704 48544769 48544704 ID=cds.MS.gene64433.t1;Parent=MS.gene64433.t1
Gene Sequence

>MS.gene64433

ATGTCACCTATGTCAACTATTCCAATTCATAAACCTGAATTTCATATCTTACTTTATAATTCATCTCCAACCCATAATTACAAATGCACCAAAATTACATCCTCATGGACTATGTAAGTTTTTCTATTTAATTTCTTTTTACAATAATTTGCAAGTTTAATTATTATGATTCTTATTAATTTTATAATTTGTTTGTGTACTATGGTTTTTTGAATTCTGTGTTCTATGGTTATTAATTATTGTTTGATATGAATAGGTCTATGGATTCAAAATTTTCTTCAACAGTGAATAGTAGTAATGATATTAAGAATAAGAAGATGAAGGATGAACTTTCTGTGAAGCTTTCAAGTCCAATTGTTCCTAAATTGGTGGAAGAAGGTTTGAGCTCAAATGGTCTTCGATTTGATCGGTTGCAGCCATCGGAACAAGAGTTGATTAGAGAAAATAGATTTGAGTTTGGGCAATTTGTAGCTAGGGAAGCTGTGCTTGATGAAGAGTTTTGGGTATGTTTTAAACTGTTGGATAGAAAGAAGCCCGTTTGAATTTGTTTATTTTTTAGCTTATGAAAAATAATTTATGCAAATAAATAAGTTTTTATGTTAATTCATGAGTTCTCACTAACTATAATTGTATCTTCATAAGCTGTTTTTTCATAAACTACTTTGACAAGTTTATGAATAATGCATAAAAGCTTATATTATTTGCATAAAATGTTTTGCATAAGCTCATAAATCAGTCAATTCAAATGGGCCCTTAGTGTATGAAATTGTATGGTTAGTCGATTCTTTTTTGAAATTAATACTAAAATGATGTTTGAAATTGAAAATTCTCAAGGTTTGTTTGATAGTGAATATTCAATTTATTGCCTGACTTGTAGGATTAGTTGATTTGGTTTTGAAATCAACAAAATTTTATTATTATGATGCTTGAAATTGTAAATCTTCGATCATCTTAGAGATTAAATAAACTGATCTAAAATGACTAGTGTGATCGTTGATTACCAAATTTCTACAATTTCATGAACTAAAATGGTGATTCACTTTGTCTGTCTTTTAGGAGTAGATTGATAAGTAAAGCTGTTTTTTAGTTGAAGATGAAAAAGAAAGAAGAAATAAAGCTTAAGAAAGAAGAAATAAAGCTTAACCTAATGTTGGAATCAAAGAAAAATTGTTTTATAAGTGCATACAACCAATCATTTATCATTCATTTGTACTTTTGTGGAAAAAATAATATACAAAAAATAGCATTTTCTTTTATTTATTGTATATTTTTGAATTCTTTTGCAGACAGCAGCCTGGCTAAGGGCAGAAAGTCATTGGGAGAATCAAACATATGAAAGGTATGATACAAAATACATCACAAATCTCCAATGTTTTGTCATAGGGTTAAATATGTTTTTAGTCCTTACATTTTGCGCCCCTTTGAATTATAGTCCCTTCATTTCATTAAATGTTTTTAAAATCCTCACATTTTATCTCTGTGATCAAAAATAGTCCCTGCTGTTAATTCTCTAACGGAATGCTGATATGGCAGTTAGTGGGTCCCAATTATTTTAGGGTTAAATATGTTTTTCATACATAAGGATTCTGTAGAATGAATTGATAAAATGTGTTCCTTTCATCGTGGTTGTTGTGTTATTGTGTAAAACTTGTGTTGAGCATATTGTGGTACTTGATTTGAGAACAGATTATAAACCAGATCAAGATGGATGATGTCAATAATCAGTTTTCTAAATAGTAGAATATAGGAAGTAATCTGAATGACCCTACCGGTTGTGGGTGTAAATCATTTGACTTGATTAACAATAGGTAAGAATTTGAGTAGTACTAACTGCAATTATGTTTTTTTTTTTAGATATATTGATATCTACAAAAGGAAATTTGCTGATCAGGTAGGAATCGGCGGTGCTTAATTTCCTTACTTAGTACTATACACTGGAGTGTCTTTCATGAAAATTTTAACAAGAATCCCTATTGTTTAGGAATTTAATGCGGTAAAAAAGCGGTGCAAGGTGCAAAATGGGGACAGCTGCGCATGCATTATCGCGGTAAATCAATCTCATCTGAGATTCTGATCTAGTTAAAAAAATTCAGGTTACAAAAAAATATATACTTTAAAGCTCTAACTTTGATTAAAGCTCTAACTTTGTTGAGTTATTTACTGTTTGAAAGTTGATTTTCCTTGACTATGATTCGACTTACGGTGTTTGGATTCATGTTTATCAGGTGAGAAAGGAGCAGAAGAATGTAAAGCGCTCGATAATAAAAAGTGTTGTAGGAACACTTGACTTGAATATCCGATATTTGCTGCAAGGAGAGACTTTTCCTGGGGTACTACTCATTCTTTCTCATCTACTGTTCTGTCTAGACTGATGGTCTTGATGTTTTTCACGACTTAGTCATCATATCATTTCTGCAGGAACGAGACAAAACTCCTTTTTGTTGCAGCATAAACAGAACACCACCAAGTAGATATGGTTACATTGCAAATTTATGTGTTGCCAAATTGGCTCGTCGACAAGGAATTGCAAGCAGCATGATGTCTTTTGCTGTTGAAAATGCAAAATCTAATGGTAATATTAACGTTAAGTTGAAGTAACTTTATGGTTTACGCCTGATTTGTGGATTCATTTAGCAGGTGTGAGTCAGGTATATGTGCATGTGGACAGAAATAATAGGCCGGGACAGTTATTGTACCAAAAGATGGGCTTTGAGGTAATGTAATTGTTGGTTCATCTGTATTTCCAGTTTTTGTTATAGATTGTTGCTACATGTGAAATCAATAACTATGGCAATTTCGCCGTCAATTTCAGATGGTTGAAACTGCAAACTCTCGGTTGTTACCAGAGGAAACATACTTGCTACGTTTACAGACGTAA
Protein sequence

>MS.gene64433.t1

MSPMSTIPIHKPEFHILLYNSSPTHNYKCTKITSSWTMSMDSKFSSTVNSSNDIKNKKMKDELSVKLSSPIVPKLVEEGLSSNGLRFDRLQPSEQELIRENRFEFGQFVAREAVLDEEFWTAAWLRAESHWENQTYERYIDIYKRKFADQEFNAVKKRCKVQNGDSCACIIAVRKEQKNVKRSIIKSVVGTLDLNIRYLLQGETFPGERDKTPFCCSINRTPPSRYGYIANLCVAKLARRQGIASSMMSFAVENAKSNGVSQVYVHVDRNNRPGQLLYQKMGFEMVETANSRLLPEETYLLRLQT