Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64502.t1 | XP_013450702.1 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 4.50E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64502.t1 | O23659 | 35.4 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.4e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64502.t1 | A0A072UG20 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.3e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene64502.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64502.t1 | MTR_6g004120 | 70.229 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.51e-65 | 195 |
| MS.gene64502.t1 | MTR_1g040600 | 45.695 | 151 | 55 | 4 | 3 | 128 | 5 | 153 | 4.83e-32 | 111 |
| MS.gene64502.t1 | MTR_1g045700 | 50.893 | 112 | 51 | 3 | 23 | 130 | 111 | 222 | 9.43e-30 | 107 |
| MS.gene64502.t1 | MTR_1g046880 | 38.889 | 162 | 62 | 5 | 5 | 129 | 9 | 170 | 1.78e-26 | 97.8 |
| MS.gene64502.t1 | MTR_2g058520 | 44.348 | 115 | 56 | 2 | 23 | 129 | 62 | 176 | 1.65e-25 | 95.5 |
| MS.gene64502.t1 | MTR_1g046720 | 43.966 | 116 | 58 | 3 | 21 | 129 | 2 | 117 | 2.52e-25 | 93.2 |
| MS.gene64502.t1 | MTR_1g046550 | 45.763 | 118 | 58 | 4 | 18 | 129 | 145 | 262 | 3.31e-25 | 96.7 |
| MS.gene64502.t1 | MTR_1g045740 | 41.322 | 121 | 63 | 3 | 22 | 134 | 144 | 264 | 3.61e-24 | 94.4 |
| MS.gene64502.t1 | MTR_1g045720 | 43.966 | 116 | 57 | 3 | 22 | 129 | 144 | 259 | 6.93e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64502.t1 | AT1G78640 | 41.228 | 114 | 58 | 2 | 25 | 129 | 154 | 267 | 1.50e-19 | 82.0 |
| MS.gene64502.t1 | AT2G33720 | 35.398 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.03e-16 | 74.3 |
| MS.gene64502.t1 | AT1G78635 | 37.705 | 122 | 65 | 4 | 26 | 139 | 121 | 239 | 5.51e-15 | 69.3 |
| MS.gene64502.t1 | AT4G02870 | 32.759 | 116 | 62 | 4 | 26 | 131 | 191 | 300 | 3.40e-11 | 59.7 |
Find 22 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATAAGATTGGTCTTTAT+TGG | 0.198682 | 7.3:-12488059 | MS.gene64502:CDS |
| TAGGAATTCGAGCTGAAATA+TGG | 0.273058 | 7.3:-12488194 | MS.gene64502:CDS |
| GGATAAGATTGGTCTTTATT+GGG | 0.344915 | 7.3:-12488058 | MS.gene64502:CDS |
| CTCAAATTCAAGAGATTGTC+TGG | 0.348648 | 7.3:-12488144 | MS.gene64502:CDS |
| GAACAGTAAAATTGAAGAAT+CGG | 0.427975 | 7.3:+12488025 | None:intergenic |
| GACACACTCCTAGAGATAAA+TGG | 0.428020 | 7.3:+12488355 | None:intergenic |
| GTTTGTGACCATTTATCTCT+AGG | 0.448904 | 7.3:-12488363 | MS.gene64502:CDS |
| TTGGGTGGTAATGTTGAGAT+AGG | 0.471139 | 7.3:-12488213 | MS.gene64502:CDS |
| AGGAATTCGAGCTGAAATAT+GGG | 0.489554 | 7.3:-12488193 | MS.gene64502:CDS |
| GTACAAGCAACGATGACTCT+TGG | 0.507283 | 7.3:-12488329 | MS.gene64502:CDS |
| TTATGGCTTATAGCAACAGT+AGG | 0.507490 | 7.3:-12488386 | MS.gene64502:CDS |
| CTTGAATTTGAGAACGTGCA+TGG | 0.514512 | 7.3:+12488155 | None:intergenic |
| GAGTCATCGTTGCTTGTACA+AGG | 0.516173 | 7.3:+12488333 | None:intergenic |
| TTAAAGCAAGGGGATAAGAT+TGG | 0.521643 | 7.3:-12488069 | MS.gene64502:CDS |
| GTGTTGACAATGAGTGATAC+CGG | 0.535346 | 7.3:-12488294 | MS.gene64502:CDS |
| CCAACAAAAGTCTTCTCATA+CGG | 0.561709 | 7.3:+12488271 | None:intergenic |
| AAAGAAGAGGCTTAAAGCAA+GGG | 0.562567 | 7.3:-12488080 | MS.gene64502:CDS |
| AAAAGAAGAGGCTTAAAGCA+AGG | 0.562720 | 7.3:-12488081 | MS.gene64502:CDS |
| TTTGTGTTGTCTGTGTTGGG+TGG | 0.569410 | 7.3:-12488228 | MS.gene64502:CDS |
| TATGGCTTATAGCAACAGTA+GGG | 0.575545 | 7.3:-12488385 | MS.gene64502:CDS |
| CAAAAGTCTTCTCATACGGC+CGG | 0.580656 | 7.3:+12488275 | None:intergenic |
| AAGAAGAGGCTTAAAGCAAG+GGG | 0.637026 | 7.3:-12488079 | MS.gene64502:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTGGAATAATAAAAT+TGG | + | chr7.3:12488378-12488397 | None:intergenic | 10.0% |
| ! | GAACAGTAAAATTGAAGAAT+CGG | + | chr7.3:12488349-12488368 | None:intergenic | 25.0% |
| !! | ACTTTTGTTGGGTAAAAAAT+TGG | - | chr7.3:12488112-12488131 | MS.gene64502:CDS | 25.0% |
| GACAAGTTTGTAAAAAGAAG+AGG | - | chr7.3:12488278-12488297 | MS.gene64502:CDS | 30.0% | |
| ! | ATATATTTTCACGGATGGTT+GGG | - | chr7.3:12488253-12488272 | MS.gene64502:CDS | 30.0% |
| ! | CTTCAATTTTACTGTTCTTG+AGG | - | chr7.3:12488352-12488371 | MS.gene64502:CDS | 30.0% |
| ! | GTCTGGATCATATATTTTCA+CGG | - | chr7.3:12488244-12488263 | MS.gene64502:CDS | 30.0% |
| !! | GGATAAGATTGGTCTTTATT+GGG | - | chr7.3:12488313-12488332 | MS.gene64502:CDS | 30.0% |
| AAAAGAAGAGGCTTAAAGCA+AGG | - | chr7.3:12488290-12488309 | MS.gene64502:CDS | 35.0% | |
| AAAGAAGAGGCTTAAAGCAA+GGG | - | chr7.3:12488291-12488310 | MS.gene64502:CDS | 35.0% | |
| AGGAATTCGAGCTGAAATAT+GGG | - | chr7.3:12488178-12488197 | MS.gene64502:CDS | 35.0% | |
| CCAACAAAAGTCTTCTCATA+CGG | + | chr7.3:12488103-12488122 | None:intergenic | 35.0% | |
| CTCAAATTCAAGAGATTGTC+TGG | - | chr7.3:12488227-12488246 | MS.gene64502:CDS | 35.0% | |
| GTTTGTGACCATTTATCTCT+AGG | - | chr7.3:12488008-12488027 | MS.gene64502:CDS | 35.0% | |
| TAGGAATTCGAGCTGAAATA+TGG | - | chr7.3:12488177-12488196 | MS.gene64502:CDS | 35.0% | |
| TTAAAGCAAGGGGATAAGAT+TGG | - | chr7.3:12488302-12488321 | MS.gene64502:CDS | 35.0% | |
| ! | CATATATTTTCACGGATGGT+TGG | - | chr7.3:12488252-12488271 | MS.gene64502:CDS | 35.0% |
| ! | CGTATGAGAAGACTTTTGTT+GGG | - | chr7.3:12488101-12488120 | MS.gene64502:CDS | 35.0% |
| ! | GATTTTGTGTTGTCTGTGTT+GGG | - | chr7.3:12488140-12488159 | MS.gene64502:CDS | 35.0% |
| ! | GGATCATATATTTTCACGGA+TGG | - | chr7.3:12488248-12488267 | MS.gene64502:CDS | 35.0% |
| !! | GGGATAAGATTGGTCTTTAT+TGG | - | chr7.3:12488312-12488331 | MS.gene64502:CDS | 35.0% |
| AAGAAGAGGCTTAAAGCAAG+GGG | - | chr7.3:12488292-12488311 | MS.gene64502:CDS | 40.0% | |
| CTTGAATTTGAGAACGTGCA+TGG | + | chr7.3:12488219-12488238 | None:intergenic | 40.0% | |
| GACACACTCCTAGAGATAAA+TGG | + | chr7.3:12488019-12488038 | None:intergenic | 40.0% | |
| GTTGGGTAAAAAATTGGCTG+AGG | - | chr7.3:12488118-12488137 | MS.gene64502:CDS | 40.0% | |
| ! | CCGTATGAGAAGACTTTTGT+TGG | - | chr7.3:12488100-12488119 | MS.gene64502:CDS | 40.0% |
| ! | GGATTTTGTGTTGTCTGTGT+TGG | - | chr7.3:12488139-12488158 | MS.gene64502:CDS | 40.0% |
| ! | GTGTTGACAATGAGTGATAC+CGG | - | chr7.3:12488077-12488096 | MS.gene64502:CDS | 40.0% |
| ! | TTGGGTGGTAATGTTGAGAT+AGG | - | chr7.3:12488158-12488177 | MS.gene64502:CDS | 40.0% |
| CAAAAGTCTTCTCATACGGC+CGG | + | chr7.3:12488099-12488118 | None:intergenic | 45.0% | |
| GAGTCATCGTTGCTTGTACA+AGG | + | chr7.3:12488041-12488060 | None:intergenic | 45.0% | |
| GTACAAGCAACGATGACTCT+TGG | - | chr7.3:12488042-12488061 | MS.gene64502:CDS | 45.0% | |
| TTTGTGTTGTCTGTGTTGGG+TGG | - | chr7.3:12488143-12488162 | MS.gene64502:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12487987 | 12488406 | 12487987 | ID=MS.gene64502 |
| chr7.3 | mRNA | 12487987 | 12488406 | 12487987 | ID=MS.gene64502.t1;Parent=MS.gene64502 |
| chr7.3 | exon | 12487987 | 12488406 | 12487987 | ID=MS.gene64502.t1.exon1;Parent=MS.gene64502.t1 |
| chr7.3 | CDS | 12487987 | 12488406 | 12487987 | ID=cds.MS.gene64502.t1;Parent=MS.gene64502.t1 |
| Gene Sequence |
| Protein sequence |
