Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64508.t1 | GAU37750.1 | 42.3 | 137 | 78 | 1 | 22 | 157 | 73 | 209 | 6.30E-22 | 114 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64508.t1 | A0A2Z6NMK1 | 42.3 | 137 | 78 | 1 | 22 | 157 | 73 | 209 | 4.5e-22 | 114.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene64508.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 7.3:+12587814 | MS.gene64508:CDS |
| CAAATGTAATGAAGGTTTCT+AGG | 0.290265 | 7.3:+12587949 | MS.gene64508:CDS |
| TCATAACCTGCAGCCATATA+AGG | 0.313221 | 7.3:-12587624 | None:intergenic |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 7.3:+12587483 | MS.gene64508:intron |
| AATGATCTTAACTCCTTATA+TGG | 0.327626 | 7.3:+12587611 | MS.gene64508:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 7.3:-12587518 | None:intergenic |
| TCAAGGACTCATAGGGATAA+TGG | 0.395623 | 7.3:+12587169 | MS.gene64508:CDS |
| GATCAACTTCAAGGACTCAT+AGG | 0.397052 | 7.3:+12587161 | MS.gene64508:CDS |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 7.3:+12587914 | MS.gene64508:CDS |
| TATGGCTGCAGGTTATGAAT+TGG | 0.411365 | 7.3:+12587629 | MS.gene64508:CDS |
| GAGTTAAGATCATTTCATAA+TGG | 0.420070 | 7.3:-12587602 | None:intergenic |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 7.3:+12587750 | MS.gene64508:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.427022 | 7.3:+12587516 | MS.gene64508:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 7.3:+12587729 | MS.gene64508:intron |
| TTCTCTGCTATTAATATGTA+TGG | 0.435001 | 7.3:+12587774 | MS.gene64508:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 7.3:+12587815 | MS.gene64508:CDS |
| GTTGTTATCAATACAATGTC+TGG | 0.455307 | 7.3:+12587133 | None:intergenic |
| TTAACTCCTTATATGGCTGC+AGG | 0.483063 | 7.3:+12587618 | MS.gene64508:CDS |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 7.3:+12587783 | MS.gene64508:CDS |
| CTGGTGGTGGATCAACTTCA+AGG | 0.486585 | 7.3:+12587152 | MS.gene64508:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 7.3:+12587885 | MS.gene64508:CDS |
| CAACAAAGATTGGCAGTGGA+TGG | 0.512212 | 7.3:+12587853 | MS.gene64508:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 7.3:+12587572 | MS.gene64508:CDS |
| AAGTACATCAAATGTAATGA+AGG | 0.524181 | 7.3:+12587941 | MS.gene64508:CDS |
| ATGTCAACTCATTGCCATCA+TGG | 0.539340 | 7.3:+12587547 | MS.gene64508:CDS |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 7.3:+12587889 | MS.gene64508:CDS |
| ATCAATACAATGTCTGGTGG+TGG | 0.560856 | 7.3:+12587139 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 7.3:+12587484 | MS.gene64508:intron |
| AAGGCAACAAAGATTGGCAG+TGG | 0.562007 | 7.3:+12587849 | MS.gene64508:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 7.3:-12587561 | None:intergenic |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 7.3:-12587798 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 7.3:-12587808 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 7.3:+12587819 | MS.gene64508:CDS |
| ATCAACTTCAAGGACTCATA+GGG | 0.600193 | 7.3:+12587162 | MS.gene64508:CDS |
| TACTGCTTATATCATCAATG+AGG | 0.608119 | 7.3:+12587201 | MS.gene64508:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 7.3:-12587902 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 7.3:+12587888 | MS.gene64508:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 7.3:+12587890 | MS.gene64508:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 7.3:+12587564 | MS.gene64508:CDS |
| GTTATCAATACAATGTCTGG+TGG | 0.684260 | 7.3:+12587136 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGCTCAACATAAAATAAT+AGG | - | chr7.3:12587236-12587255 | None:intergenic | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | + | chr7.3:12587876-12587895 | MS.gene64508:CDS | 20.0% |
| ! | AAGTACATCAAATGTAATGA+AGG | + | chr7.3:12587941-12587960 | MS.gene64508:CDS | 25.0% |
| ! | AATGATCTTAACTCCTTATA+TGG | + | chr7.3:12587611-12587630 | MS.gene64508:CDS | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | + | chr7.3:12587482-12587501 | MS.gene64508:intron | 25.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | + | chr7.3:12587814-12587833 | MS.gene64508:CDS | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | + | chr7.3:12587483-12587502 | MS.gene64508:intron | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | + | chr7.3:12587815-12587834 | MS.gene64508:CDS | 25.0% |
| ! | GAGTTAAGATCATTTCATAA+TGG | - | chr7.3:12587605-12587624 | None:intergenic | 25.0% |
| ! | TTCTCTGCTATTAATATGTA+TGG | + | chr7.3:12587774-12587793 | MS.gene64508:CDS | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | chr7.3:12587914-12587933 | MS.gene64508:CDS | 25.0% |
| !! | TAGAGATGTATCATTTTAGA+AGG | - | chr7.3:12587670-12587689 | None:intergenic | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | chr7.3:12587843-12587862 | MS.gene64508:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | + | chr7.3:12587830-12587849 | MS.gene64508:CDS | 30.0% | |
| ATAAAATGCTCAACAAAGGA+AGG | - | chr7.3:12587323-12587342 | None:intergenic | 30.0% | |
| ATATCTCATTTACAACCGTA+TGG | + | chr7.3:12587287-12587306 | MS.gene64508:intron | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | + | chr7.3:12587783-12587802 | MS.gene64508:CDS | 30.0% | |
| CAAATGTAATGAAGGTTTCT+AGG | + | chr7.3:12587949-12587968 | MS.gene64508:CDS | 30.0% | |
| GAACTTAACTTTGTGTCTAT+TGG | - | chr7.3:12587385-12587404 | None:intergenic | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | + | chr7.3:12587201-12587220 | MS.gene64508:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | - | chr7.3:12587811-12587830 | None:intergenic | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | + | chr7.3:12587484-12587503 | MS.gene64508:intron | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | + | chr7.3:12587444-12587463 | MS.gene64508:intron | 30.0% |
| ! | GTTGATAAAATGCTCAACAA+AGG | - | chr7.3:12587327-12587346 | None:intergenic | 30.0% |
| !! | ATCATTTTAGAAGGAGTTGT+TGG | - | chr7.3:12587661-12587680 | None:intergenic | 30.0% |
| ATCAACTTCAAGGACTCATA+GGG | + | chr7.3:12587162-12587181 | MS.gene64508:CDS | 35.0% | |
| TGTGATGTACTAATAAGGGA+TGG | + | chr7.3:12587819-12587838 | MS.gene64508:CDS | 35.0% | |
| ! | TGTAAAGACAATGGATTCCA+TGG | + | chr7.3:12587885-12587904 | MS.gene64508:CDS | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | + | chr7.3:12587448-12587467 | MS.gene64508:intron | 35.0% |
| ATGTCAACTCATTGCCATCA+TGG | + | chr7.3:12587547-12587566 | MS.gene64508:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | + | chr7.3:12587572-12587591 | MS.gene64508:CDS | 40.0% | |
| GAAGGTCTAATCTCTCCATA+CGG | - | chr7.3:12587305-12587324 | None:intergenic | 40.0% | |
| GATCAACTTCAAGGACTCAT+AGG | + | chr7.3:12587161-12587180 | MS.gene64508:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | + | chr7.3:12587750-12587769 | MS.gene64508:CDS | 40.0% | |
| TATGGCTGCAGGTTATGAAT+TGG | + | chr7.3:12587629-12587648 | MS.gene64508:CDS | 40.0% | |
| TCAAGGACTCATAGGGATAA+TGG | + | chr7.3:12587169-12587188 | MS.gene64508:CDS | 40.0% | |
| TCATAACCTGCAGCCATATA+AGG | - | chr7.3:12587627-12587646 | None:intergenic | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | + | chr7.3:12587434-12587453 | MS.gene64508:intron | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | + | chr7.3:12587618-12587637 | MS.gene64508:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | + | chr7.3:12587516-12587535 | MS.gene64508:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | + | chr7.3:12587888-12587907 | MS.gene64508:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | + | chr7.3:12587889-12587908 | MS.gene64508:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | + | chr7.3:12587729-12587748 | MS.gene64508:intron | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | chr7.3:12587849-12587868 | MS.gene64508:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | - | chr7.3:12587905-12587924 | None:intergenic | 45.0% | |
| CAACAAAGATTGGCAGTGGA+TGG | + | chr7.3:12587853-12587872 | MS.gene64508:CDS | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | + | chr7.3:12587433-12587452 | MS.gene64508:intron | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | + | chr7.3:12587417-12587436 | MS.gene64508:intron | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | - | chr7.3:12587521-12587540 | None:intergenic | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | chr7.3:12587564-12587583 | MS.gene64508:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | + | chr7.3:12587416-12587435 | MS.gene64508:intron | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | chr7.3:12587564-12587583 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | + | chr7.3:12587890-12587909 | MS.gene64508:CDS | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | + | chr7.3:12587421-12587440 | MS.gene64508:intron | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | - | chr7.3:12587426-12587445 | None:intergenic | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | chr7.3:12587801-12587820 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCAACTTCA+AGG | + | chr7.3:12587152-12587171 | MS.gene64508:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12587148 | 12587977 | 12587148 | ID=MS.gene64508 |
| chr7.3 | mRNA | 12587148 | 12587977 | 12587148 | ID=MS.gene64508.t1;Parent=MS.gene64508 |
| chr7.3 | exon | 12587148 | 12587222 | 12587148 | ID=MS.gene64508.t1.exon1;Parent=MS.gene64508.t1 |
| chr7.3 | CDS | 12587148 | 12587222 | 12587148 | ID=cds.MS.gene64508.t1;Parent=MS.gene64508.t1 |
| chr7.3 | exon | 12587498 | 12587650 | 12587498 | ID=MS.gene64508.t1.exon2;Parent=MS.gene64508.t1 |
| chr7.3 | CDS | 12587498 | 12587650 | 12587498 | ID=cds.MS.gene64508.t1;Parent=MS.gene64508.t1 |
| chr7.3 | exon | 12587732 | 12587977 | 12587732 | ID=MS.gene64508.t1.exon3;Parent=MS.gene64508.t1 |
| chr7.3 | CDS | 12587732 | 12587977 | 12587732 | ID=cds.MS.gene64508.t1;Parent=MS.gene64508.t1 |
| Gene Sequence |
| Protein sequence |
