AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64578

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64578.t1 MTR_7g059400 93.201 353 19 1 1 353 1 348 0.0 558
MS.gene64578.t1 MTR_4g022370 48.042 383 126 16 1 340 1 353 1.21e-87 268
MS.gene64578.t1 MTR_4g063780 44.753 324 148 11 1 302 1 315 1.77e-69 221
MS.gene64578.t1 MTR_2g096740 40.647 278 105 11 1 256 1 240 1.84e-51 173
MS.gene64578.t1 MTR_2g096740 40.385 260 116 12 1 256 1 225 1.76e-45 157
MS.gene64578.t1 MTR_7g024670 55.479 146 57 4 1 142 1 142 1.03e-44 157
MS.gene64578.t1 MTR_3g090430 63.750 80 29 0 57 136 32 111 1.16e-30 119
MS.gene64578.t1 MTR_8g479350 54.000 100 40 1 77 170 69 168 1.56e-30 119
MS.gene64578.t1 MTR_3g091820 66.667 75 21 1 57 131 28 98 1.40e-29 115
MS.gene64578.t1 MTR_2g093220 78.689 61 13 0 70 130 34 94 3.40e-29 114
MS.gene64578.t1 MTR_5g031440 58.716 109 40 1 73 181 40 143 4.72e-29 115
MS.gene64578.t1 MTR_3g077750 62.500 88 26 3 43 126 27 111 5.61e-29 114
MS.gene64578.t1 MTR_8g068210 71.875 64 18 0 72 135 28 91 1.22e-28 114
MS.gene64578.t1 MTR_4g461080 79.661 59 12 0 70 128 23 81 1.54e-28 112
MS.gene64578.t1 MTR_8g015840 75.362 69 14 2 68 136 13 78 2.71e-28 110
MS.gene64578.t1 MTR_8g079060 44.156 154 54 6 55 207 17 139 5.38e-28 109
MS.gene64578.t1 MTR_2g014060 66.197 71 23 1 58 127 6 76 7.24e-28 111
MS.gene64578.t1 MTR_1g056810 80.357 56 11 0 75 130 11 66 8.85e-28 110
MS.gene64578.t1 MTR_4g109980 88.000 50 6 0 77 126 41 90 2.37e-27 110
MS.gene64578.t1 MTR_8g027295 78.947 57 12 0 78 134 26 82 2.91e-27 108
MS.gene64578.t1 MTR_2g014170 87.500 48 6 0 77 124 46 93 5.29e-26 106
MS.gene64578.t1 MTR_2g013370 63.889 72 17 2 69 132 31 101 1.01e-25 104
MS.gene64578.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 1.01e-25 104
MS.gene64578.t1 MTR_4g089095 80.000 55 10 1 78 132 52 105 1.13e-25 105
MS.gene64578.t1 MTR_4g089095 80.000 55 10 1 78 132 18 71 1.29e-25 103
MS.gene64578.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 1.30e-25 104
MS.gene64578.t1 MTR_2g059540 87.500 48 6 0 77 124 32 79 2.14e-25 102
MS.gene64578.t1 MTR_6g012450 71.429 56 16 0 78 133 138 193 2.57e-23 100
MS.gene64578.t1 MTR_7g086780 44.737 114 52 2 77 179 100 213 4.34e-23 99.8
MS.gene64578.t1 MTR_2g016030 53.247 77 33 1 78 154 49 122 8.19e-23 93.6
MS.gene64578.t1 MTR_3g435480 68.421 57 18 0 78 134 138 194 1.11e-22 99.0
MS.gene64578.t1 MTR_7g010950 61.194 67 26 0 78 144 137 203 1.41e-22 99.0
MS.gene64578.t1 MTR_8g044220 68.519 54 17 0 78 131 120 173 2.08e-22 97.8
MS.gene64578.t1 MTR_6g027450 59.677 62 23 1 71 130 82 143 1.12e-21 94.7
MS.gene64578.t1 MTR_4g082060 69.231 52 16 0 78 129 124 175 1.29e-21 95.9
MS.gene64578.t1 MTR_5g041400 58.667 75 25 1 78 152 121 189 1.42e-21 94.7
MS.gene64578.t1 MTR_5g041420 61.290 62 24 0 78 139 140 201 1.66e-21 94.0
MS.gene64578.t1 MTR_5g041380 66.102 59 20 0 78 136 129 187 6.22e-21 93.2
MS.gene64578.t1 MTR_6g027460 57.377 61 24 1 71 129 89 149 8.31e-21 92.8
MS.gene64578.t1 MTR_5g041530 69.231 52 16 0 78 129 142 193 1.21e-20 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64578.t1 AT3G55370 37.245 392 145 18 1 338 1 345 8.61e-51 173
MS.gene64578.t1 AT3G55370 38.757 338 125 14 1 301 1 293 1.40e-49 169
MS.gene64578.t1 AT2G37590 41.544 272 106 9 1 236 1 255 2.92e-46 160
MS.gene64578.t1 AT3G55370 39.252 321 112 14 48 338 92 359 1.62e-44 157
MS.gene64578.t1 AT5G02460 85.526 76 11 0 55 130 74 149 3.23e-43 154
MS.gene64578.t1 AT1G07640 72.152 79 22 0 58 136 3 81 3.98e-36 132
MS.gene64578.t1 AT1G07640 72.152 79 22 0 58 136 59 137 6.97e-36 133
MS.gene64578.t1 AT1G07640 72.152 79 22 0 58 136 67 145 8.14e-36 133
MS.gene64578.t1 AT2G28810 46.053 152 50 6 1 127 1 145 1.08e-33 127
MS.gene64578.t1 AT5G60200 67.073 82 24 1 73 151 52 133 4.36e-33 124
MS.gene64578.t1 AT2G28510 85.246 61 9 0 75 135 47 107 5.92e-32 121
MS.gene64578.t1 AT5G60850 61.957 92 32 1 63 154 38 126 6.48e-32 122
MS.gene64578.t1 AT2G28810 73.239 71 18 1 58 127 58 128 1.51e-31 121
MS.gene64578.t1 AT3G52440 71.014 69 20 0 73 141 22 90 1.18e-30 117
MS.gene64578.t1 AT3G52440 71.014 69 20 0 73 141 41 109 1.82e-30 117
MS.gene64578.t1 AT3G45610 67.143 70 23 0 73 142 37 106 8.13e-30 115
MS.gene64578.t1 AT5G65590 54.639 97 37 2 35 131 7 96 1.11e-29 116
MS.gene64578.t1 AT1G21340 62.353 85 30 1 78 162 40 122 1.39e-29 114
MS.gene64578.t1 AT3G21270 48.905 137 57 3 77 213 30 153 1.87e-29 112
MS.gene64578.t1 AT1G51700 77.612 67 13 2 69 134 27 92 3.16e-29 112
MS.gene64578.t1 AT1G28310 82.143 56 10 0 77 132 28 83 1.20e-28 113
MS.gene64578.t1 AT5G62940 48.438 128 59 4 1 126 1 123 1.47e-28 114
MS.gene64578.t1 AT1G28310 82.143 56 10 0 77 132 52 107 1.77e-28 113
MS.gene64578.t1 AT5G66940 52.252 111 41 4 37 145 2 102 4.21e-28 109
MS.gene64578.t1 AT4G21050 39.881 168 73 4 74 213 22 189 4.29e-28 109
MS.gene64578.t1 AT3G61850 45.985 137 55 5 10 133 1 131 1.61e-27 110
MS.gene64578.t1 AT3G61850 45.985 137 55 5 10 133 1 131 2.55e-27 110
MS.gene64578.t1 AT3G61850 54.455 101 41 3 38 133 19 119 2.56e-27 109
MS.gene64578.t1 AT3G61850 54.455 101 41 3 38 133 19 119 2.56e-27 109
MS.gene64578.t1 AT3G61850 73.016 63 15 1 73 133 57 119 3.83e-27 109
MS.gene64578.t1 AT4G24060 72.131 61 17 0 70 130 47 107 4.00e-27 110
MS.gene64578.t1 AT3G50410 55.682 88 36 2 49 133 1 88 4.68e-27 107
MS.gene64578.t1 AT4G38000 75.439 57 14 0 70 126 35 91 6.12e-27 107
MS.gene64578.t1 AT4G00940 74.576 59 14 1 72 130 65 122 1.06e-25 105
MS.gene64578.t1 AT4G00940 74.576 59 14 1 72 130 65 122 1.06e-25 105
MS.gene64578.t1 AT4G00940 74.576 59 14 1 72 130 65 122 1.06e-25 105
MS.gene64578.t1 AT2G46590 77.358 53 10 1 73 123 63 115 1.64e-23 100
MS.gene64578.t1 AT2G46590 77.358 53 10 1 73 123 75 127 1.73e-23 100
MS.gene64578.t1 AT3G47500 63.636 66 24 0 78 143 112 177 1.91e-23 101
MS.gene64578.t1 AT1G64620 47.020 151 59 5 70 213 43 179 2.34e-23 99.8
MS.gene64578.t1 AT5G62430 73.077 52 14 0 78 129 56 107 3.34e-23 98.2
MS.gene64578.t1 AT2G34140 71.154 52 15 0 78 129 60 111 2.16e-22 92.8
MS.gene64578.t1 AT1G29160 71.154 52 15 0 78 129 64 115 2.16e-22 93.2
MS.gene64578.t1 AT1G47655 78.723 47 10 0 78 124 31 77 2.98e-22 93.6
MS.gene64578.t1 AT1G26790 69.231 52 16 0 78 129 113 164 5.63e-22 96.3
MS.gene64578.t1 AT1G69570 71.154 52 15 0 78 129 134 185 1.06e-21 95.5
MS.gene64578.t1 AT4G21040 70.909 55 15 1 78 132 27 80 2.02e-21 92.0
MS.gene64578.t1 AT5G39660 71.154 52 15 0 78 129 140 191 2.28e-21 95.1
MS.gene64578.t1 AT5G39660 71.154 52 15 0 78 129 140 191 2.28e-21 95.1
MS.gene64578.t1 AT5G39660 71.154 52 15 0 78 129 140 191 2.28e-21 95.1
MS.gene64578.t1 AT4G21080 53.165 79 33 2 78 156 27 101 9.84e-21 90.5
MS.gene64578.t1 AT4G21030 42.667 75 43 0 74 148 19 93 7.19e-14 70.1

Find 111 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCAACCGGGACACTTCTA+AGG 0.192890 7.3:+45150369 None:intergenic
TACTTCTCTCCCACCATATA+TGG 0.222301 7.3:-45150869 MS.gene64578:CDS
TTCCAATATTGTTCACTATT+AGG 0.263046 7.3:+45149774 None:intergenic
AAATAATTCTAGTGTGAAAA+TGG 0.280136 7.3:-45149877 MS.gene64578:CDS
ATCTCATCGGTGGTGGCGTT+GGG 0.300347 7.3:+45150231 None:intergenic
TTCCATATCGGAAGCTCTTT+AGG 0.302834 7.3:-45150113 MS.gene64578:CDS
GGTGATCACCTAAGTGAAAT+AGG 0.308942 7.3:-45150185 MS.gene64578:CDS
ATCCTAATAGTGAACAATAT+TGG 0.316935 7.3:-45149776 MS.gene64578:CDS
GAATCAATCTAGACAATTCT+TGG 0.322625 7.3:-45149850 MS.gene64578:CDS
GCAAGGCTTGTCGAAGGTAT+TGG 0.355195 7.3:-45150406 MS.gene64578:CDS
CACCTAAAGAGCTTCCGATA+TGG 0.360566 7.3:+45150111 None:intergenic
AGTTGTTTGTAGAGCTAGTT+TGG 0.376308 7.3:+45150273 None:intergenic
CTTCGGCAACCACCGCCAAC+CGG 0.399525 7.3:+45150356 None:intergenic
CATGAGGTTGCGATGGTGTC+AGG 0.399816 7.3:+45150582 None:intergenic
ACCTGTTGATGCCAGTTGGC+TGG 0.401216 7.3:+45150844 None:intergenic
CAGTTGGCTGGATCCATATA+TGG 0.404780 7.3:+45150856 None:intergenic
AATCTCATCGGTGGTGGCGT+TGG 0.416328 7.3:+45150230 None:intergenic
GGAGACTTAAATTTCCATAT+CGG 0.426564 7.3:-45150125 MS.gene64578:CDS
TCCAGCCAACTGGCATCAAC+AGG 0.444071 7.3:-45150845 MS.gene64578:intron
AAGGGAAATTGATGAGTTTG+TGG 0.457842 7.3:+45150020 None:intergenic
CCGCTACCACCACCTTCAAA+TGG 0.459662 7.3:+45149954 None:intergenic
AGTGAAATAGGACTAAACTA+TGG 0.460875 7.3:-45150173 MS.gene64578:CDS
GCCCTATCTGCCATCGAACC+TGG 0.462544 7.3:+45150545 None:intergenic
CTACTACGAATTCCAACTAT+AGG 0.462952 7.3:-45149680 MS.gene64578:CDS
TCCAGTCCAATGGGAGGTGT+TGG 0.464011 7.3:-45150146 MS.gene64578:CDS
GCCACCACCGATGAGATTCA+TGG 0.464462 7.3:-45150225 MS.gene64578:CDS
AATCAATCTAGACAATTCTT+GGG 0.469852 7.3:-45149849 MS.gene64578:CDS
GACCAGGTTCGATGGCAGAT+AGG 0.475722 7.3:-45150547 MS.gene64578:CDS
TGCGTATGGTGGTGTGTCTA+AGG 0.477118 7.3:-45149907 MS.gene64578:CDS
CTTAGAAGTGTCCCGGTTGG+CGG 0.481122 7.3:-45150368 MS.gene64578:CDS
CATATATGGATCCAGCCAAC+TGG 0.482920 7.3:-45150855 MS.gene64578:CDS
ATGGCTCCTTTGCATCATCT+AGG 0.483090 7.3:-45150206 MS.gene64578:CDS
ATAAAGTTATGTCCTATAGT+TGG 0.484745 7.3:+45149668 None:intergenic
ACCAGGTTCGATGGCAGATA+GGG 0.488883 7.3:-45150546 MS.gene64578:CDS
GAAGGTGGTGGTAGCGGTGG+TGG 0.489455 7.3:-45149948 MS.gene64578:CDS
AGGTTCGATGGTTGAGGCTG+TGG 0.490154 7.3:+45150602 None:intergenic
TTCGACAAGCCTTGCAGAAG+TGG 0.490902 7.3:+45150414 None:intergenic
CATTGCTCAAAATTGCCAAC+AGG 0.493075 7.3:+45150050 None:intergenic
AGAAGTGTCCCGGTTGGCGG+TGG 0.494695 7.3:-45150365 MS.gene64578:CDS
CCATTTGAAGGTGGTGGTAG+CGG 0.496097 7.3:-45149954 MS.gene64578:CDS
GGAGGCGTTGGTAACGGTGG+TGG 0.500223 7.3:-45150089 MS.gene64578:CDS
TTAGGTGGAGGCGTTGGTAA+CGG 0.512562 7.3:-45150095 MS.gene64578:CDS
AGTTTAGTCCTATTTCACTT+AGG 0.513240 7.3:+45150177 None:intergenic
GATGGTTGAGGCTGTGGTGG+TGG 0.518267 7.3:+45150608 None:intergenic
GTAGCAAAACTTTGTGTTGG+TGG 0.519405 7.3:+45150466 None:intergenic
GTCAGAGCTAGCCGGCGACC+TGG 0.520799 7.3:+45150298 None:intergenic
GGATTGTTGTATCCATTTGA+AGG 0.522126 7.3:-45149966 MS.gene64578:CDS
TGGAATGCTTCTGCTGGTGG+TGG 0.522977 7.3:-45149756 MS.gene64578:CDS
TCCAACACCTCCCATTGGAC+TGG 0.527498 7.3:+45150145 None:intergenic
TGCGATGGTGTCAGGTTCGA+TGG 0.530120 7.3:+45150590 None:intergenic
AGCTTCTAAAGTTGACAAGA+AGG 0.532028 7.3:+45150001 None:intergenic
GTAGTAGCAAAACTTTGTGT+TGG 0.542073 7.3:+45150463 None:intergenic
TTCGGCAACCACCGCCAACC+GGG 0.543263 7.3:+45150357 None:intergenic
AGCTCTTTAGGTGGAGGCGT+TGG 0.544707 7.3:-45150101 MS.gene64578:CDS
CTGACACCATCGCAACCTCA+TGG 0.545229 7.3:-45150581 MS.gene64578:CDS
CTCGATCAGACCAGGTTCGA+TGG 0.546965 7.3:-45150555 MS.gene64578:CDS
GCCATGAATCTCATCGGTGG+TGG 0.547604 7.3:+45150224 None:intergenic
CATGGAGGCTCGATCAGACC+AGG 0.549550 7.3:-45150563 MS.gene64578:CDS
GTGTTGGTGGATTCACACCT+TGG 0.549683 7.3:+45150479 None:intergenic
TTGTATCCATTTGAAGGTGG+TGG 0.552400 7.3:-45149960 MS.gene64578:CDS
TGTCGAAGGTATTGGACAAA+GGG 0.552845 7.3:-45150398 MS.gene64578:CDS
GCAGCCACGCCACTTCTGCA+AGG 0.554356 7.3:-45150423 MS.gene64578:CDS
GCCCTTAGAAGTGTCCCGGT+TGG 0.556141 7.3:-45150371 MS.gene64578:CDS
ATCGGAAGCTCTTTAGGTGG+AGG 0.559518 7.3:-45150107 MS.gene64578:CDS
ATTTACCTGTTGATGCCAGT+TGG 0.559637 7.3:+45150840 None:intergenic
CAATATTGGAATGCTTCTGC+TGG 0.566637 7.3:-45149762 MS.gene64578:CDS
TGGTGCCCTTAGAAGTGTCC+CGG 0.567606 7.3:-45150375 MS.gene64578:CDS
TTCGATGGTTGAGGCTGTGG+TGG 0.570012 7.3:+45150605 None:intergenic
GTTGGGGAACTGAGTACCGC+AGG 0.570283 7.3:+45150248 None:intergenic
CATATCGGAAGCTCTTTAGG+TGG 0.571600 7.3:-45150110 MS.gene64578:CDS
AAGTCTCCAACACCTCCCAT+TGG 0.574092 7.3:+45150140 None:intergenic
TGTGGTGGTGGAATAAGTAG+AGG 0.574363 7.3:+45150620 None:intergenic
GATGGCAGATAGGGCAAGGA+TGG 0.575021 7.3:-45150537 MS.gene64578:CDS
TAGACACACCACCATACGCA+TGG 0.575174 7.3:+45149910 None:intergenic
TCTCATCGGTGGTGGCGTTG+GGG 0.576658 7.3:+45150232 None:intergenic
GCCAACCGGGACACTTCTAA+GGG 0.580076 7.3:+45150370 None:intergenic
GTTTGGCGGTCAGAGCTAGC+CGG 0.580677 7.3:+45150290 None:intergenic
AGAGCTAGCCGGCGACCTGG+AGG 0.581045 7.3:+45150301 None:intergenic
TTAGAAGCTTCTTCTTCACA+TGG 0.581519 7.3:-45149987 MS.gene64578:CDS
TATTGGAATGCTTCTGCTGG+TGG 0.586587 7.3:-45149759 MS.gene64578:CDS
TAACAACACCTCCAGGTCGC+CGG 0.588755 7.3:-45150309 MS.gene64578:CDS
GTGGTTGCCGAAGAAACAAA+AGG 0.588845 7.3:-45150346 MS.gene64578:CDS
GCTTCTAAAGTTGACAAGAA+GGG 0.589264 7.3:+45150002 None:intergenic
TGTTTGTAGAGCTAGTTTGG+CGG 0.589509 7.3:+45150276 None:intergenic
CAAGCCTTGCAGAAGTGGCG+TGG 0.593138 7.3:+45150419 None:intergenic
CAAGCAGTAACAACACCTCC+AGG 0.593324 7.3:-45150316 MS.gene64578:CDS
GCAGCAACAAAATCATCAAG+TGG 0.596192 7.3:-45150663 MS.gene64578:intron
TTGTCGAAGGTATTGGACAA+AGG 0.596657 7.3:-45150399 MS.gene64578:CDS
GGTGGAGGCGTTGGTAACGG+TGG 0.597906 7.3:-45150092 MS.gene64578:CDS
TTTGAAGGTGGTGGTAGCGG+TGG 0.603652 7.3:-45149951 MS.gene64578:CDS
GAGCCTCCATGAGGTTGCGA+TGG 0.604696 7.3:+45150575 None:intergenic
AGGAGTGTTGACACTAGTGT+TGG 0.605835 7.3:+45150640 None:intergenic
GGTGTCAGGTTCGATGGTTG+AGG 0.608734 7.3:+45150596 None:intergenic
TTGGCTGGATCCATATATGG+TGG 0.609224 7.3:+45150859 None:intergenic
GTTCGATGGCAGATAGGGCA+AGG 0.614474 7.3:-45150541 MS.gene64578:CDS
TTGTTGTATCCATTTGAAGG+TGG 0.620036 7.3:-45149963 MS.gene64578:CDS
TGATCACCTAGATGATGCAA+AGG 0.622493 7.3:+45150200 None:intergenic
CGAAGGTATTGGACAAAGGG+TGG 0.625409 7.3:-45150395 MS.gene64578:CDS
TGGCTGGATCCATATATGGT+GGG 0.628650 7.3:+45150860 None:intergenic
AAAGGAGCCATGAATCTCAT+CGG 0.638198 7.3:+45150218 None:intergenic
ACTTCTGCAAGGCTTGTCGA+AGG 0.638852 7.3:-45150412 MS.gene64578:CDS
GCTGTAGAAAGATCTGTCCA+AGG 0.641214 7.3:+45149720 None:intergenic
GTGGTGCTACTAATTCACCT+TGG 0.642878 7.3:-45149737 MS.gene64578:CDS
AGACACACCACCATACGCAT+GGG 0.645170 7.3:+45149911 None:intergenic
AATGATCAATCCCATGCGTA+TGG 0.649437 7.3:-45149921 MS.gene64578:CDS
GATCAATCCCATGCGTATGG+TGG 0.672453 7.3:-45149918 MS.gene64578:CDS
CTCTACAAACAACTCTCCTG+CGG 0.682618 7.3:-45150264 MS.gene64578:CDS
GGTCTGATCGAGCCTCCATG+AGG 0.689453 7.3:+45150566 None:intergenic
GGAGCCATGAATCTCATCGG+TGG 0.692277 7.3:+45150221 None:intergenic
TGGGAGAGAAGTATAAACCA+TGG 0.714065 7.3:+45150879 None:intergenic
ACACCATCGCAACCTCATGG+AGG 0.720835 7.3:-45150578 MS.gene64578:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAATAAAAATTAA+TGG + chr7.3:45149867-45149886 None:intergenic 10.0%
!! GATACATAAAATCAAATATT+TGG + chr7.3:45149840-45149859 None:intergenic 15.0%
!! AAATAATTCTAGTGTGAAAA+TGG - chr7.3:45150674-45150693 MS.gene64578:intron 20.0%
! AATCAATCTAGACAATTCTT+GGG - chr7.3:45150702-45150721 MS.gene64578:intron 25.0%
! ATCCTAATAGTGAACAATAT+TGG - chr7.3:45150775-45150794 MS.gene64578:intron 25.0%
! ATTTGGTATATTCAAGAGAA+GGG + chr7.3:45149823-45149842 None:intergenic 25.0%
! TATTTGGTATATTCAAGAGA+AGG + chr7.3:45149824-45149843 None:intergenic 25.0%
! TTCCAATATTGTTCACTATT+AGG + chr7.3:45150780-45150799 None:intergenic 25.0%
!!! ATTTAGACCTTTTGTTTCTT+CGG + chr7.3:45150215-45150234 None:intergenic 25.0%
AGTGAAATAGGACTAAACTA+TGG - chr7.3:45150378-45150397 MS.gene64578:CDS 30.0%
AGTTTAGTCCTATTTCACTT+AGG + chr7.3:45150377-45150396 None:intergenic 30.0%
ATCAACAGGTAAATCAACAA+GGG - chr7.3:45149720-45149739 MS.gene64578:CDS 30.0%
GAATCAATCTAGACAATTCT+TGG - chr7.3:45150701-45150720 MS.gene64578:intron 30.0%
GGAGACTTAAATTTCCATAT+CGG - chr7.3:45150426-45150445 MS.gene64578:CDS 30.0%
! TGATTTTGTTGCTGCAAAAA+AGG + chr7.3:45149883-45149902 None:intergenic 30.0%
AACCATCACAAAAATGTCCA+AGG - chr7.3:45150055-45150074 MS.gene64578:CDS 35.0%
AAGGGAAATTGATGAGTTTG+TGG + chr7.3:45150534-45150553 None:intergenic 35.0%
AGTTGTTTGTAGAGCTAGTT+TGG + chr7.3:45150281-45150300 None:intergenic 35.0%
CATCAACAGGTAAATCAACA+AGG - chr7.3:45149719-45149738 MS.gene64578:CDS 35.0%
CTACTACGAATTCCAACTAT+AGG - chr7.3:45150871-45150890 MS.gene64578:CDS 35.0%
GGATTGTTGTATCCATTTGA+AGG - chr7.3:45150585-45150604 MS.gene64578:CDS 35.0%
GTAGTAGCAAAACTTTGTGT+TGG + chr7.3:45150091-45150110 None:intergenic 35.0%
TCAACAAGGGTTCTTCAATT+AGG - chr7.3:45149733-45149752 MS.gene64578:CDS 35.0%
TTAGAAGCTTCTTCTTCACA+TGG - chr7.3:45150564-45150583 MS.gene64578:CDS 35.0%
TTGTTGTATCCATTTGAAGG+TGG - chr7.3:45150588-45150607 MS.gene64578:CDS 35.0%
! TATGGTTTTTCCAGTCCAAT+GGG - chr7.3:45150396-45150415 MS.gene64578:CDS 35.0%
! TCTACTTCGATTTTACCTGT+TGG - chr7.3:45150486-45150505 MS.gene64578:CDS 35.0%
! TTTTGTGATGGTTCTTGCAT+AGG + chr7.3:45150048-45150067 None:intergenic 35.0%
!! AGCTTCTAAAGTTGACAAGA+AGG + chr7.3:45150553-45150572 None:intergenic 35.0%
!! GCTTCTAAAGTTGACAAGAA+GGG + chr7.3:45150552-45150571 None:intergenic 35.0%
AAAGGAGCCATGAATCTCAT+CGG + chr7.3:45150336-45150355 None:intergenic 40.0%
AATGATCAATCCCATGCGTA+TGG - chr7.3:45150630-45150649 MS.gene64578:CDS 40.0%
ATTTACCTGTTGATGCCAGT+TGG + chr7.3:45149714-45149733 None:intergenic 40.0%
CAATATTGGAATGCTTCTGC+TGG - chr7.3:45150789-45150808 MS.gene64578:intron 40.0%
CATTGCTCAAAATTGCCAAC+AGG + chr7.3:45150504-45150523 None:intergenic 40.0%
GCAGCAACAAAATCATCAAG+TGG - chr7.3:45149888-45149907 MS.gene64578:CDS 40.0%
GGTGATCACCTAAGTGAAAT+AGG - chr7.3:45150366-45150385 MS.gene64578:CDS 40.0%
GTAGCAAAACTTTGTGTTGG+TGG + chr7.3:45150088-45150107 None:intergenic 40.0%
TACTTCTCTCCCACCATATA+TGG - chr7.3:45149682-45149701 MS.gene64578:CDS 40.0%
TGATCACCTAGATGATGCAA+AGG + chr7.3:45150354-45150373 None:intergenic 40.0%
TTCCATATCGGAAGCTCTTT+AGG - chr7.3:45150438-45150457 MS.gene64578:CDS 40.0%
TTGTATCCATTTGAAGGTGG+TGG - chr7.3:45150591-45150610 MS.gene64578:CDS 40.0%
! CACCTTGGACATTTTTGTGA+TGG + chr7.3:45150060-45150079 None:intergenic 40.0%
! CTATGGTTTTTCCAGTCCAA+TGG - chr7.3:45150395-45150414 MS.gene64578:CDS 40.0%
! CTGTTGGCAATTTTGAGCAA+TGG - chr7.3:45150502-45150521 MS.gene64578:CDS 40.0%
! TGTTTGTAGAGCTAGTTTGG+CGG + chr7.3:45150278-45150297 None:intergenic 40.0%
! TTGGCAATTTTGAGCAATGG+AGG - chr7.3:45150505-45150524 MS.gene64578:CDS 40.0%
!! TGTCGAAGGTATTGGACAAA+GGG - chr7.3:45150153-45150172 MS.gene64578:CDS 40.0%
!! TTGTCGAAGGTATTGGACAA+AGG - chr7.3:45150152-45150171 MS.gene64578:CDS 40.0%
ATGGCTCCTTTGCATCATCT+AGG - chr7.3:45150345-45150364 MS.gene64578:CDS 45.0%
CACCTAAAGAGCTTCCGATA+TGG + chr7.3:45150443-45150462 None:intergenic 45.0%
CAGTTGGCTGGATCCATATA+TGG + chr7.3:45149698-45149717 None:intergenic 45.0%
CATATATGGATCCAGCCAAC+TGG - chr7.3:45149696-45149715 MS.gene64578:CDS 45.0%
CATATCGGAAGCTCTTTAGG+TGG - chr7.3:45150441-45150460 MS.gene64578:CDS 45.0%
CTCTACAAACAACTCTCCTG+CGG - chr7.3:45150287-45150306 MS.gene64578:CDS 45.0%
GCTGTAGAAAGATCTGTCCA+AGG + chr7.3:45150834-45150853 None:intergenic 45.0%
GTGGTTGCCGAAGAAACAAA+AGG - chr7.3:45150205-45150224 MS.gene64578:CDS 45.0%
TATTGGAATGCTTCTGCTGG+TGG - chr7.3:45150792-45150811 MS.gene64578:intron 45.0%
TGGCTGGATCCATATATGGT+GGG + chr7.3:45149694-45149713 None:intergenic 45.0%
TTGGCTGGATCCATATATGG+TGG + chr7.3:45149695-45149714 None:intergenic 45.0%
!! AGGAGTGTTGACACTAGTGT+TGG + chr7.3:45149914-45149933 None:intergenic 45.0%
!! GTGGTGCTACTAATTCACCT+TGG - chr7.3:45150814-45150833 MS.gene64578:intron 45.0%
!! TGTGGTGGTGGAATAAGTAG+AGG + chr7.3:45149934-45149953 None:intergenic 45.0%
AAGTCTCCAACACCTCCCAT+TGG + chr7.3:45150414-45150433 None:intergenic 50.0%
ACCAGGTTCGATGGCAGATA+GGG - chr7.3:45150005-45150024 MS.gene64578:CDS 50.0%
ACTTCTGCAAGGCTTGTCGA+AGG - chr7.3:45150139-45150158 MS.gene64578:CDS 50.0%
AGACACACCACCATACGCAT+GGG + chr7.3:45150643-45150662 None:intergenic 50.0%
ATCGGAAGCTCTTTAGGTGG+AGG - chr7.3:45150444-45150463 MS.gene64578:CDS 50.0%
CAAGCAGTAACAACACCTCC+AGG - chr7.3:45150235-45150254 MS.gene64578:CDS 50.0%
GATCAATCCCATGCGTATGG+TGG - chr7.3:45150633-45150652 MS.gene64578:CDS 50.0%
TAGACACACCACCATACGCA+TGG + chr7.3:45150644-45150663 None:intergenic 50.0%
TTCGACAAGCCTTGCAGAAG+TGG + chr7.3:45150140-45150159 None:intergenic 50.0%
! CCATTTGAAGGTGGTGGTAG+CGG - chr7.3:45150597-45150616 MS.gene64578:CDS 50.0%
! GCAAGGCTTGTCGAAGGTAT+TGG - chr7.3:45150145-45150164 MS.gene64578:CDS 50.0%
! GGTTTTTCCAGTCCAATGGG+AGG - chr7.3:45150399-45150418 MS.gene64578:CDS 50.0%
! TGCGTATGGTGGTGTGTCTA+AGG - chr7.3:45150644-45150663 MS.gene64578:CDS 50.0%
!! CGAAGGTATTGGACAAAGGG+TGG - chr7.3:45150156-45150175 MS.gene64578:CDS 50.0%
!! GTGTTGGTGGATTCACACCT+TGG + chr7.3:45150075-45150094 None:intergenic 50.0%
!! TTAGGTGGAGGCGTTGGTAA+CGG - chr7.3:45150456-45150475 MS.gene64578:CDS 50.0%
ACACCATCGCAACCTCATGG+AGG - chr7.3:45149973-45149992 MS.gene64578:CDS 55.0%
ACCTGTTGATGCCAGTTGGC+TGG + chr7.3:45149710-45149729 None:intergenic 55.0%
AGGTTCGATGGTTGAGGCTG+TGG + chr7.3:45149952-45149971 None:intergenic 55.0%
CATGAGGTTGCGATGGTGTC+AGG + chr7.3:45149972-45149991 None:intergenic 55.0%
CCGCTACCACCACCTTCAAA+TGG + chr7.3:45150600-45150619 None:intergenic 55.0%
CTCGATCAGACCAGGTTCGA+TGG - chr7.3:45149996-45150015 MS.gene64578:CDS 55.0%
CTGACACCATCGCAACCTCA+TGG - chr7.3:45149970-45149989 MS.gene64578:CDS 55.0%
GACCAGGTTCGATGGCAGAT+AGG - chr7.3:45150004-45150023 MS.gene64578:CDS 55.0%
GATGGCAGATAGGGCAAGGA+TGG - chr7.3:45150014-45150033 MS.gene64578:CDS 55.0%
GCCAACCGGGACACTTCTAA+GGG + chr7.3:45150184-45150203 None:intergenic 55.0%
GCCACCACCGATGAGATTCA+TGG - chr7.3:45150326-45150345 MS.gene64578:CDS 55.0%
GCCATGAATCTCATCGGTGG+TGG + chr7.3:45150330-45150349 None:intergenic 55.0%
GGAGCCATGAATCTCATCGG+TGG + chr7.3:45150333-45150352 None:intergenic 55.0%
GGTGTCAGGTTCGATGGTTG+AGG + chr7.3:45149958-45149977 None:intergenic 55.0%
GTTCGATGGCAGATAGGGCA+AGG - chr7.3:45150010-45150029 MS.gene64578:CDS 55.0%
TAACAACACCTCCAGGTCGC+CGG - chr7.3:45150242-45150261 MS.gene64578:CDS 55.0%
TCCAACACCTCCCATTGGAC+TGG + chr7.3:45150409-45150428 None:intergenic 55.0%
TCCAGCCAACTGGCATCAAC+AGG - chr7.3:45149706-45149725 MS.gene64578:CDS 55.0%
TGGAATGCTTCTGCTGGTGG+TGG - chr7.3:45150795-45150814 MS.gene64578:intron 55.0%
TTCGATGGTTGAGGCTGTGG+TGG + chr7.3:45149949-45149968 None:intergenic 55.0%
! AATCTCATCGGTGGTGGCGT+TGG + chr7.3:45150324-45150343 None:intergenic 55.0%
! AGCTCTTTAGGTGGAGGCGT+TGG - chr7.3:45150450-45150469 MS.gene64578:CDS 55.0%
! ATCTCATCGGTGGTGGCGTT+GGG + chr7.3:45150323-45150342 None:intergenic 55.0%
! TCCAGTCCAATGGGAGGTGT+TGG - chr7.3:45150405-45150424 MS.gene64578:CDS 55.0%
! TGCGATGGTGTCAGGTTCGA+TGG + chr7.3:45149964-45149983 None:intergenic 55.0%
!! CTTAGAAGTGTCCCGGTTGG+CGG - chr7.3:45150183-45150202 MS.gene64578:CDS 55.0%
!! TGGTGCCCTTAGAAGTGTCC+CGG - chr7.3:45150176-45150195 MS.gene64578:CDS 55.0%
!! TTTGAAGGTGGTGGTAGCGG+TGG - chr7.3:45150600-45150619 MS.gene64578:CDS 55.0%
CAAGCCTTGCAGAAGTGGCG+TGG + chr7.3:45150135-45150154 None:intergenic 60.0%
CATGGAGGCTCGATCAGACC+AGG - chr7.3:45149988-45150007 MS.gene64578:CDS 60.0%
CGCCAACCGGGACACTTCTA+AGG + chr7.3:45150185-45150204 None:intergenic 60.0%
GAGCCTCCATGAGGTTGCGA+TGG + chr7.3:45149979-45149998 None:intergenic 60.0%
GATGGTTGAGGCTGTGGTGG+TGG + chr7.3:45149946-45149965 None:intergenic 60.0%
GCCCTATCTGCCATCGAACC+TGG + chr7.3:45150009-45150028 None:intergenic 60.0%
GGTCTGATCGAGCCTCCATG+AGG + chr7.3:45149988-45150007 None:intergenic 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG + chr7.3:45150264-45150283 None:intergenic 60.0%
! GTTGGGGAACTGAGTACCGC+AGG + chr7.3:45150306-45150325 None:intergenic 60.0%
! TCTCATCGGTGGTGGCGTTG+GGG + chr7.3:45150322-45150341 None:intergenic 60.0%
!! GCCCTTAGAAGTGTCCCGGT+TGG - chr7.3:45150180-45150199 MS.gene64578:CDS 60.0%
CTTCGGCAACCACCGCCAAC+CGG + chr7.3:45150198-45150217 None:intergenic 65.0%
GCAGCCACGCCACTTCTGCA+AGG - chr7.3:45150128-45150147 MS.gene64578:CDS 65.0%
TTCGGCAACCACCGCCAACC+GGG + chr7.3:45150197-45150216 None:intergenic 65.0%
!! AGAAGTGTCCCGGTTGGCGG+TGG - chr7.3:45150186-45150205 MS.gene64578:CDS 65.0%
!! GAAGGTGGTGGTAGCGGTGG+TGG - chr7.3:45150603-45150622 MS.gene64578:CDS 65.0%
!! GGAGGCGTTGGTAACGGTGG+TGG - chr7.3:45150462-45150481 MS.gene64578:CDS 65.0%
!! GGTGGAGGCGTTGGTAACGG+TGG - chr7.3:45150459-45150478 MS.gene64578:CDS 65.0%
AGAGCTAGCCGGCGACCTGG+AGG + chr7.3:45150253-45150272 None:intergenic 70.0%
GTCAGAGCTAGCCGGCGACC+TGG + chr7.3:45150256-45150275 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 45149674 45150899 45149674 ID=MS.gene64578
chr7.3 mRNA 45149674 45150899 45149674 ID=MS.gene64578.t1;Parent=MS.gene64578
chr7.3 exon 45150846 45150899 45150846 ID=MS.gene64578.t1.exon1;Parent=MS.gene64578.t1
chr7.3 CDS 45150846 45150899 45150846 ID=cds.MS.gene64578.t1;Parent=MS.gene64578.t1
chr7.3 exon 45149674 45150681 45149674 ID=MS.gene64578.t1.exon2;Parent=MS.gene64578.t1
chr7.3 CDS 45149674 45150681 45149674 ID=cds.MS.gene64578.t1;Parent=MS.gene64578.t1
Gene Sequence

>MS.gene64578

ATGGTTTATACTTCTCTCCCACCATATATGGATCCAGCCAACTGGCATCAACAGGTAAATCAACAAGGGTTCTTCAATTAGGTTTCAAAAAGAAAGAAAAAAAATAAACTCAAAAGATAAATATTCGAGCTTTACTTTTGTTTATACCCTTCTCTTGAATATACCAAATATTTGATTTTATGTATCTTTACCATTAATTTTTATTTCCTTTTTTGCAGCAACAAAATCATCAAGTGGCCAACACTAGTGTCAACACTCCTCTACTTATTCCACCACCACAGCCTCAACCATCGAACCTGACACCATCGCAACCTCATGGAGGCTCGATCAGACCAGGTTCGATGGCAGATAGGGCAAGGATGGCAAACATACCTATGCAAGAACCATCACAAAAATGTCCAAGGTGTGAATCCACCAACACAAAGTTTTGCTACTACAACAACTATAGTCTCTCGCAGCCACGCCACTTCTGCAAGGCTTGTCGAAGGTATTGGACAAAGGGTGGTGCCCTTAGAAGTGTCCCGGTTGGCGGTGGTTGCCGAAGAAACAAAAGGTCTAAATCAAGCAGTAACAACACCTCCAGGTCGCCGGCTAGCTCTGACCGCCAAACTAGCTCTACAAACAACTCTCCTGCGGTACTCAGTTCCCCAACGCCACCACCGATGAGATTCATGGCTCCTTTGCATCATCTAGGTGATCACCTAAGTGAAATAGGACTAAACTATGGTTTTTCCAGTCCAATGGGAGGTGTTGGAGACTTAAATTTCCATATCGGAAGCTCTTTAGGTGGAGGCGTTGGTAACGGTGGTGGTTCTACTTCGATTTTACCTGTTGGCAATTTTGAGCAATGGAGGATGCCACAAACTCATCAATTTCCCTTCTTGTCAACTTTAGAAGCTTCTTCTTCACATGGATTGTTGTATCCATTTGAAGGTGGTGGTAGCGGTGGTGGTGCTAATGATCAATCCCATGCGTATGGTGGTGTGTCTAAGGTATTATCAAATAATTCTAGTGTGAAAATGGAAGAGAATCAATCTAGACAATTCTTGGGAATGATCAACAAAAACAACATCAATAATAATAATAATAATAATAATAATAATCCTAATAGTGAACAATATTGGAATGCTTCTGCTGGTGGTGGTGCTACTAATTCACCTTGGACAGATCTTTCTACAGCTTATAACACTTCTTCAGCTACTACGAATTCCAACTATAGGACATAA
Protein sequence

>MS.gene64578.t1

MVYTSLPPYMDPANWHQQQQNHQVANTSVNTPLLIPPPQPQPSNLTPSQPHGGSIRPGSMADRARMANIPMQEPSQKCPRCESTNTKFCYYNNYSLSQPRHFCKACRRYWTKGGALRSVPVGGGCRRNKRSKSSSNNTSRSPASSDRQTSSTNNSPAVLSSPTPPPMRFMAPLHHLGDHLSEIGLNYGFSSPMGGVGDLNFHIGSSLGGGVGNGGGSTSILPVGNFEQWRMPQTHQFPFLSTLEASSSHGLLYPFEGGGSGGGANDQSHAYGGVSKVLSNNSSVKMEENQSRQFLGMINKNNINNNNNNNNNNPNSEQYWNASAGGGATNSPWTDLSTAYNTSSATTNSNYRT