AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64660.t1 MTR_3g005460 65.306 245 61 3 1 222 1 244 2.95e-102 306
MS.gene64660.t1 MTR_3g005460 46.667 255 87 3 16 221 257 511 4.88e-69 220
MS.gene64660.t1 MTR_3g005420 65.289 242 64 2 1 222 1 242 1.38e-99 298
MS.gene64660.t1 MTR_3g005420 46.698 212 107 1 16 221 265 476 1.54e-52 176
MS.gene64660.t1 MTR_3g005420 65.289 242 64 2 1 222 20 261 8.12e-99 297
MS.gene64660.t1 MTR_3g005420 48.988 247 85 1 16 221 284 530 8.85e-72 228
MS.gene64660.t1 MTR_3g005490 55.731 253 80 2 1 221 1 253 1.37e-91 270
MS.gene64660.t1 MTR_5g070440 53.435 262 81 1 1 221 1 262 1.94e-90 267
MS.gene64660.t1 MTR_3g005495 68.844 199 49 2 36 222 28 225 6.03e-88 267
MS.gene64660.t1 MTR_3g005495 47.660 235 92 4 16 222 238 469 1.51e-64 207
MS.gene64660.t1 MTR_3g005480 53.150 254 86 2 1 221 1 254 4.83e-86 256
MS.gene64660.t1 MTR_3g005440 54.854 206 47 2 35 221 34 212 3.84e-63 202
MS.gene64660.t1 MTR_3g005440 49.246 199 67 2 16 180 237 435 8.07e-57 186
MS.gene64660.t1 MTR_1g015350 37.736 212 89 4 1 209 1 172 4.11e-28 105
MS.gene64660.t1 MTR_1g034240 41.414 99 58 0 21 119 7 105 2.48e-24 100
MS.gene64660.t1 MTR_4g068320 35.294 119 70 1 9 127 1 112 1.01e-20 89.7
MS.gene64660.t1 MTR_7g050560 31.579 133 87 1 9 137 1 133 2.53e-20 87.4
MS.gene64660.t1 MTR_3g061310 43.434 99 54 1 21 119 12 108 7.02e-20 85.9
MS.gene64660.t1 MTR_1g034210 37.037 108 67 1 21 128 7 113 2.62e-19 84.7
MS.gene64660.t1 MTR_1g034210 37.037 108 67 1 21 128 7 113 3.74e-19 85.1
MS.gene64660.t1 MTR_3g061320 43.000 100 55 1 20 119 11 108 6.42e-19 83.2
MS.gene64660.t1 MTR_3g061350 37.097 124 75 2 21 144 12 132 2.26e-18 81.6
MS.gene64660.t1 MTR_7g050580 30.000 140 95 2 9 147 1 138 2.88e-18 82.0
MS.gene64660.t1 MTR_3g061290 41.000 100 57 1 20 119 11 108 3.97e-18 79.7
MS.gene64660.t1 MTR_3g061370 40.777 103 59 1 17 119 5 105 4.42e-18 80.9
MS.gene64660.t1 MTR_7g050710 31.343 134 84 1 9 134 1 134 9.68e-18 80.5
MS.gene64660.t1 MTR_3g061330 41.414 99 56 1 21 119 12 108 1.36e-17 79.0
MS.gene64660.t1 MTR_5g044670 34.314 102 67 0 18 119 13 114 3.00e-17 79.3
MS.gene64660.t1 MTR_3g098790 32.407 108 73 0 14 121 6 113 5.51e-17 78.6
MS.gene64660.t1 MTR_7g050720 31.683 101 69 0 20 120 9 109 1.49e-15 74.3
MS.gene64660.t1 MTR_3g061340 43.000 100 54 2 21 119 12 109 1.93e-15 73.6
MS.gene64660.t1 MTR_7g050590 32.990 97 63 1 37 131 2 98 4.19e-14 70.1
MS.gene64660.t1 MTR_3g061220 38.384 99 46 2 21 119 12 95 4.40e-14 66.6
MS.gene64660.t1 MTR_8g103940 29.293 99 70 0 21 119 39 137 2.92e-12 65.5
MS.gene64660.t1 MTR_1g033740 27.660 141 97 3 20 160 21 156 7.51e-12 62.4
MS.gene64660.t1 MTR_1g035460 34.579 107 64 2 37 143 2 102 9.76e-12 63.9
MS.gene64660.t1 MTR_7g050640 26.852 108 79 0 14 121 6 113 1.31e-11 60.1
MS.gene64660.t1 MTR_1g108780 28.704 108 72 1 36 138 12 119 2.38e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64660.t1 AT1G49475 37.600 125 75 2 5 129 20 141 1.61e-20 85.5
MS.gene64660.t1 AT3G18990 38.384 99 61 0 21 119 6 104 2.32e-20 88.2
MS.gene64660.t1 AT3G18990 38.384 99 61 0 21 119 6 104 4.84e-20 87.4
MS.gene64660.t1 AT4G01580 36.111 108 69 0 14 121 23 130 3.15e-19 82.4
MS.gene64660.t1 AT3G18960 35.185 108 70 0 14 121 23 130 1.21e-18 80.9
MS.gene64660.t1 AT3G18960 35.185 108 70 0 14 121 23 130 2.40e-18 80.5
MS.gene64660.t1 AT3G18960 34.091 88 58 0 34 121 6 93 4.15e-13 65.5

Find 55 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCTTTCCTTGAGAATTT+TGG 0.150373 5.2:-67814970 None:intergenic
GATGAGTTTATAACAAGATT+TGG 0.242784 5.2:+67814493 MS.gene64660:CDS
AGTGAAGCATACTATTTCTT+TGG 0.281017 5.2:-67813706 None:intergenic
ATTTCTTATCCATTCAAAAC+TGG 0.281992 5.2:+67814727 MS.gene64660:CDS
ATGAGTTTATAACAAGATTT+GGG 0.360502 5.2:+67814494 MS.gene64660:CDS
TTCAAAACTGGGAAGACTAA+TGG 0.374313 5.2:+67814739 MS.gene64660:CDS
TGGAGCTTGAACAGAACATT+TGG 0.381563 5.2:-67814950 None:intergenic
TACTATTTCTTTGGTATGCA+TGG 0.398817 5.2:-67813697 None:intergenic
AATCTTGTTATAAACTCATC+TGG 0.413404 5.2:-67814490 None:intergenic
AAAGCAATGCGAATAACGTT+TGG 0.418391 5.2:+67815028 MS.gene64660:CDS
GAACACTACTCTATCTGTTA+TGG 0.428310 5.2:+67814631 MS.gene64660:CDS
TATGTAGATTTCAAGTATGA+AGG 0.431237 5.2:+67814658 MS.gene64660:CDS
ATTTCTTTGGTATGCATGGG+AGG 0.447169 5.2:-67813693 None:intergenic
TCCTTCTCCGGCGCCGGTGA+CGG 0.453830 5.2:-67813639 None:intergenic
TGACGGTTGCGAATGGCATA+TGG 0.456852 5.2:+67814552 MS.gene64660:CDS
TCCGTCACCGGCGCCGGAGA+AGG 0.467872 5.2:+67813638 MS.gene64660:CDS
TTCTTTGGTATGCATGGGAG+GGG 0.472658 5.2:-67813691 None:intergenic
TTGGAATCCTTCTCCGGCGC+CGG 0.476077 5.2:-67813645 None:intergenic
GAGAAGATTTGTTCCGTCAC+CGG 0.477470 5.2:+67813626 MS.gene64660:CDS
AAATGTTTGGAATCCTTCTC+CGG 0.487728 5.2:-67813651 None:intergenic
ACTATTTCTTTGGTATGCAT+GGG 0.496981 5.2:-67813696 None:intergenic
ATATCGACAGTCAAAGGATT+GGG 0.499850 5.2:-67814794 None:intergenic
TATTCTGCGGCGAATTCAGC+AGG 0.501814 5.2:-67814920 None:intergenic
ATTTGTTCCGTCACCGGCGC+CGG 0.504505 5.2:+67813632 MS.gene64660:CDS
TTGGATTCTTTAGATTTGCA+AGG 0.513403 5.2:+67815047 MS.gene64660:CDS
CATATCGACAGTCAAAGGAT+TGG 0.519552 5.2:-67814795 None:intergenic
ATTGTGTATTGAACAAAGGC+GGG 0.520442 5.2:+67815002 MS.gene64660:CDS
AAGGATTGGGAACGAAAAGA+AGG 0.522583 5.2:-67814781 None:intergenic
ATAGAGTAGTGTTCTGCAAA+CGG 0.529825 5.2:-67814622 None:intergenic
ACAAAACAATGCAGCCTCGC+CGG 0.533798 5.2:+67813604 None:intergenic
CACCGCATATCGACAGTCAA+AGG 0.544452 5.2:-67814800 None:intergenic
AGGGAAAACAATCTATCAGA+TGG 0.548736 5.2:+67815067 MS.gene64660:CDS
ATGTAGATTTCAAGTATGAA+GGG 0.551054 5.2:+67814659 MS.gene64660:CDS
TTCAAGCTCCAAAATTCTCA+AGG 0.552648 5.2:+67814962 MS.gene64660:CDS
TTTCTTTGGTATGCATGGGA+GGG 0.553788 5.2:-67813692 None:intergenic
GTGACGGAACAAATCTTCTC+CGG 0.557768 5.2:-67813623 None:intergenic
TTTCTTATCCATTCAAAACT+GGG 0.560037 5.2:+67814728 MS.gene64660:CDS
TCTTTGGTATGCATGGGAGG+GGG 0.563298 5.2:-67813690 None:intergenic
TGGATTCTTTAGATTTGCAA+GGG 0.565236 5.2:+67815048 MS.gene64660:CDS
GTTTATTGTGTATTGAACAA+AGG 0.570068 5.2:+67814998 MS.gene64660:CDS
CTGTTACTGACGGTTGCGAA+TGG 0.573643 5.2:+67814545 MS.gene64660:CDS
TATTGTGTATTGAACAAAGG+CGG 0.588560 5.2:+67815001 MS.gene64660:CDS
AAAATTCTCAAGGAAAGCAG+TGG 0.599450 5.2:+67814972 MS.gene64660:CDS
GCCACGATTACTGTTACTGA+CGG 0.599776 5.2:+67814535 MS.gene64660:CDS
AGGATTGGGAACGAAAAGAA+GGG 0.605751 5.2:-67814780 None:intergenic
TGTGTATTGAACAAAGGCGG+GGG 0.614183 5.2:+67815004 MS.gene64660:CDS
ATTTGGCTTCAAATATTCTG+CGG 0.631419 5.2:-67814933 None:intergenic
ATCCTTTGACTGTCGATATG+CGG 0.632326 5.2:+67814798 MS.gene64660:CDS
AAATTCTCAAGGAAAGCAGT+GGG 0.637805 5.2:+67814973 MS.gene64660:CDS
ACCGTCAGTAACAGTAATCG+TGG 0.641873 5.2:-67814536 None:intergenic
TTGTGTATTGAACAAAGGCG+GGG 0.654751 5.2:+67815003 MS.gene64660:CDS
ACGAGTTTGAGCTTATCAAG+AGG 0.654788 5.2:+67815098 MS.gene64660:CDS
CATATGGAAGTGAAGAAATG+TGG 0.688821 5.2:+67814568 MS.gene64660:CDS
GGAACAAATCTTCTCCGGCG+AGG 0.698114 5.2:-67813618 None:intergenic
GTAACTTAGAACAGAACACG+CGG 0.742535 5.2:-67815124 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGTTTATAACAAGATTT+GGG + chr5.2:67814494-67814513 MS.gene64660:CDS 20.0%
!!! AAGTTTTCTTTTGCAAAAAT+TGG + chr5.2:67814596-67814615 MS.gene64660:CDS 20.0%
!!! AATGGTTTTCATAAAATGTT+TGG - chr5.2:67813667-67813686 None:intergenic 20.0%
!!! AGTAATTTTTTTTACAACCT+TGG - chr5.2:67814252-67814271 None:intergenic 20.0%
!!! GTTTGAGTTATATATTTATG+CGG + chr5.2:67814859-67814878 MS.gene64660:intron 20.0%
! ATTTCTTATCCATTCAAAAC+TGG + chr5.2:67814727-67814746 MS.gene64660:CDS 25.0%
! GAGAGTAATTACTTAAATTG+TGG + chr5.2:67814414-67814433 MS.gene64660:intron 25.0%
! GATGAGTTTATAACAAGATT+TGG + chr5.2:67814493-67814512 MS.gene64660:CDS 25.0%
! GTTTATTGTGTATTGAACAA+AGG + chr5.2:67814998-67815017 MS.gene64660:CDS 25.0%
! TTTCTTATCCATTCAAAACT+GGG + chr5.2:67814728-67814747 MS.gene64660:CDS 25.0%
!! AATCTTGTTATAAACTCATC+TGG - chr5.2:67814493-67814512 None:intergenic 25.0%
!! ATGTAGATTTCAAGTATGAA+GGG + chr5.2:67814659-67814678 MS.gene64660:CDS 25.0%
!! GGATTTTACTGTACATTATT+TGG + chr5.2:67814824-67814843 MS.gene64660:intron 25.0%
!! TACTGATTGTACAATTTGTT+TGG + chr5.2:67813924-67813943 MS.gene64660:intron 25.0%
!! TAGTTGACTTTATATGTATG+AGG - chr5.2:67813961-67813980 None:intergenic 25.0%
!! TATGTAGATTTCAAGTATGA+AGG + chr5.2:67814658-67814677 MS.gene64660:CDS 25.0%
AATGTAGCGAAATTATGACA+TGG - chr5.2:67814058-67814077 None:intergenic 30.0%
TATTGTGTATTGAACAAAGG+CGG + chr5.2:67815001-67815020 MS.gene64660:CDS 30.0%
TCTAATGCATGCACAATTTA+TGG - chr5.2:67813758-67813777 None:intergenic 30.0%
TGGATTCTTTAGATTTGCAA+GGG + chr5.2:67815048-67815067 MS.gene64660:CDS 30.0%
TTGGATTCTTTAGATTTGCA+AGG + chr5.2:67815047-67815066 MS.gene64660:CDS 30.0%
! ACTATTTCTTTGGTATGCAT+GGG - chr5.2:67813699-67813718 None:intergenic 30.0%
! AGTGAAGCATACTATTTCTT+TGG - chr5.2:67813709-67813728 None:intergenic 30.0%
! ATTTGGCTTCAAATATTCTG+CGG - chr5.2:67814936-67814955 None:intergenic 30.0%
! TACTATTTCTTTGGTATGCA+TGG - chr5.2:67813700-67813719 None:intergenic 30.0%
!! TCAAGTCTTAGTTTCAAGTT+AGG + chr5.2:67814312-67814331 MS.gene64660:intron 30.0%
!! TGAGAATGACTTAAGTTGAA+TGG + chr5.2:67814277-67814296 MS.gene64660:intron 30.0%
!!! AACGAAAAGAAGGGTTTTTT+GGG - chr5.2:67814774-67814793 None:intergenic 30.0%
AAAATTCTCAAGGAAAGCAG+TGG + chr5.2:67814972-67814991 MS.gene64660:CDS 35.0%
AAAGCAATGCGAATAACGTT+TGG + chr5.2:67815028-67815047 MS.gene64660:CDS 35.0%
AAATTCTCAAGGAAAGCAGT+GGG + chr5.2:67814973-67814992 MS.gene64660:CDS 35.0%
AGGGAAAACAATCTATCAGA+TGG + chr5.2:67815067-67815086 MS.gene64660:CDS 35.0%
ATAGAGTAGTGTTCTGCAAA+CGG - chr5.2:67814625-67814644 None:intergenic 35.0%
ATATCGACAGTCAAAGGATT+GGG - chr5.2:67814797-67814816 None:intergenic 35.0%
ATTGTGTATTGAACAAAGGC+GGG + chr5.2:67815002-67815021 MS.gene64660:CDS 35.0%
CATATGGAAGTGAAGAAATG+TGG + chr5.2:67814568-67814587 MS.gene64660:CDS 35.0%
GAACACTACTCTATCTGTTA+TGG + chr5.2:67814631-67814650 MS.gene64660:CDS 35.0%
GGAGGAACAATCTAATTAAG+TGG - chr5.2:67814885-67814904 None:intergenic 35.0%
TTAGTGTGTCTTTCATTCCA+AGG + chr5.2:67814232-67814251 MS.gene64660:intron 35.0%
TTCAAAACTGGGAAGACTAA+TGG + chr5.2:67814739-67814758 MS.gene64660:CDS 35.0%
TTCAAGCTCCAAAATTCTCA+AGG + chr5.2:67814962-67814981 MS.gene64660:CDS 35.0%
TTTGCATGTTTGGACATTCA+AGG + chr5.2:67814193-67814212 MS.gene64660:intron 35.0%
! AAATGTTTGGAATCCTTCTC+CGG - chr5.2:67813654-67813673 None:intergenic 35.0%
! ACTGCTTTCCTTGAGAATTT+TGG - chr5.2:67814973-67814992 None:intergenic 35.0%
! TGTGACTCTTTTTGCATGTT+TGG + chr5.2:67814183-67814202 MS.gene64660:intron 35.0%
!! ATCACCATTCCTCTAAAGTT+TGG + chr5.2:67814452-67814471 MS.gene64660:intron 35.0%
!! CACAAAAAGAGATTTTCGAG+CGG - chr5.2:67814134-67814153 None:intergenic 35.0%
!!! AAAGAAGGGTTTTTTGGGTT+TGG - chr5.2:67814769-67814788 None:intergenic 35.0%
!!! GAACGAAAAGAAGGGTTTTT+TGG - chr5.2:67814775-67814794 None:intergenic 35.0%
!!! TTAGTCTTCCCAGTTTTGAA+TGG - chr5.2:67814739-67814758 None:intergenic 35.0%
AAGGATTGGGAACGAAAAGA+AGG - chr5.2:67814784-67814803 None:intergenic 40.0%
ACGAGTTTGAGCTTATCAAG+AGG + chr5.2:67815098-67815117 MS.gene64660:CDS 40.0%
AGCAGGAACATACTACACAA+TGG - chr5.2:67814906-67814925 None:intergenic 40.0%
AGGAACATACTACACAATGG+AGG - chr5.2:67814903-67814922 None:intergenic 40.0%
AGGATTGGGAACGAAAAGAA+GGG - chr5.2:67814783-67814802 None:intergenic 40.0%
ATCCTTTGACTGTCGATATG+CGG + chr5.2:67814798-67814817 MS.gene64660:CDS 40.0%
ATTTCTTTGGTATGCATGGG+AGG - chr5.2:67813696-67813715 None:intergenic 40.0%
CATATCGACAGTCAAAGGAT+TGG - chr5.2:67814798-67814817 None:intergenic 40.0%
GGTAACTGAAAACGAAAGTG+AGG - chr5.2:67813737-67813756 None:intergenic 40.0%
TGGAGCTTGAACAGAACATT+TGG - chr5.2:67814953-67814972 None:intergenic 40.0%
TTGTGTATTGAACAAAGGCG+GGG + chr5.2:67815003-67815022 MS.gene64660:CDS 40.0%
TTTCTTTGGTATGCATGGGA+GGG - chr5.2:67813695-67813714 None:intergenic 40.0%
! GTGACCAAACTTTAGAGGAA+TGG - chr5.2:67814459-67814478 None:intergenic 40.0%
ACCGTCAGTAACAGTAATCG+TGG - chr5.2:67814539-67814558 None:intergenic 45.0%
CTGCAGTGACCAAACTTTAG+AGG - chr5.2:67814464-67814483 None:intergenic 45.0%
GCCACGATTACTGTTACTGA+CGG + chr5.2:67814535-67814554 MS.gene64660:CDS 45.0%
GCTAAGTGCGTGTTTGGATT+CGG - chr5.2:67814381-67814400 None:intergenic 45.0%
TGTGTATTGAACAAAGGCGG+GGG + chr5.2:67815004-67815023 MS.gene64660:CDS 45.0%
TTCTTTGGTATGCATGGGAG+GGG - chr5.2:67813694-67813713 None:intergenic 45.0%
! GAGAAGATTTGTTCCGTCAC+CGG + chr5.2:67813626-67813645 MS.gene64660:CDS 45.0%
! GTGACGGAACAAATCTTCTC+CGG - chr5.2:67813626-67813645 None:intergenic 45.0%
CACCGCATATCGACAGTCAA+AGG - chr5.2:67814803-67814822 None:intergenic 50.0%
CTGTTACTGACGGTTGCGAA+TGG + chr5.2:67814545-67814564 MS.gene64660:CDS 50.0%
GCTTCAGCTAAGTGCGTGTT+TGG - chr5.2:67814387-67814406 None:intergenic 50.0%
TATTCTGCGGCGAATTCAGC+AGG - chr5.2:67814923-67814942 None:intergenic 50.0%
TCTTTGGTATGCATGGGAGG+GGG - chr5.2:67813693-67813712 None:intergenic 50.0%
TGACGGTTGCGAATGGCATA+TGG + chr5.2:67814552-67814571 MS.gene64660:CDS 50.0%
TTGACTGTCGATATGCGGTG+AGG + chr5.2:67814803-67814822 MS.gene64660:intron 50.0%
ATGCATGGGAGGGGGAAGAA+TGG - chr5.2:67813685-67813704 None:intergenic 55.0%
GGAACAAATCTTCTCCGGCG+AGG - chr5.2:67813621-67813640 None:intergenic 55.0%
ATTTGTTCCGTCACCGGCGC+CGG + chr5.2:67813632-67813651 MS.gene64660:CDS 60.0%
TTGGAATCCTTCTCCGGCGC+CGG - chr5.2:67813648-67813667 None:intergenic 60.0%
!! TCCTTCTCCGGCGCCGGTGA+CGG - chr5.2:67813642-67813661 None:intergenic 70.0%
! TCCGTCACCGGCGCCGGAGA+AGG + chr5.2:67813638-67813657 MS.gene64660:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 67813612 67815141 67813612 ID=MS.gene64660
chr5.2 mRNA 67813612 67815141 67813612 ID=MS.gene64660.t1;Parent=MS.gene64660
chr5.2 exon 67813612 67813716 67813612 ID=MS.gene64660.t1.exon1;Parent=MS.gene64660.t1
chr5.2 CDS 67813612 67813716 67813612 ID=cds.MS.gene64660.t1;Parent=MS.gene64660.t1
chr5.2 exon 67814484 67814819 67814484 ID=MS.gene64660.t1.exon2;Parent=MS.gene64660.t1
chr5.2 CDS 67814484 67814819 67814484 ID=cds.MS.gene64660.t1;Parent=MS.gene64660.t1
chr5.2 exon 67814914 67815141 67814914 ID=MS.gene64660.t1.exon3;Parent=MS.gene64660.t1
chr5.2 CDS 67814914 67815141 67814914 ID=cds.MS.gene64660.t1;Parent=MS.gene64660.t1
Gene Sequence

>MS.gene64660

ATGCAGCCTCGCCGGAGAAGATTTGTTCCGTCACCGGCGCCGGAGAAGGATTCCAAACATTTTATGAAAACCATTCTTCCCCCTCCCATGCATACCAAAGAAATAGTATGCTTCACTTTCTACCTCACTTTCGTTTTCAGTTACCATAAATTGTGCATGCATTAGATTGTTTTGTTCTTTTATTATGCATGCATGTCAATAATGTATTTTTATTAAATCATAAAATGTGCTTGTGCTCGAAAATGAAATAGTCATTTTCATTGTTAAATGTGTAATAAGTAGTCACTCAATGTAGCGAAATTTCAAAATAGTTACTGATTGTACAATTTGTTTGGTTAAAATAGTTCCTCATACATATAAAGTCAACTAGAATTAATTAAAAGTAGTTTTTAGATTCAGCGACGTAAAAGATTATTGTTACTAACAGATCGCACAATCAGAGACCATGTCATAATTTCGCTACATTGAAAGACTAGTATGACTACTCTTACACATTCAATGACGAAAATGACTATTTGTCCGCTCGAAAATCTCTTTTTGTGATTGTTTTCTGAATCTGTTTTAGATAATGTGTGACTCTTTTTGCATGTTTGGACATTCAAGGTAATCAGTCATTCTGTTTAGTGTGTCTTTCATTCCAAGGTTGTAAAAAAAATTACTTTTACTGAGAATGACTTAAGTTGAATGGAGTTAGAAAGCTTCAAGTCTTAGTTTCAAGTTAGGATTTATTCTAGAGTCAAAATCAATTCTACTTAAACATGTCAAACCGAATCCAAACACGCACTTAGCTGAAGCTTTTTATGAGAGTAATTACTTAAATTGTGGAAATATCATAAATATATCACCATTCCTCTAAAGTTTGGTCACTGCAGAGAATTCCAGATGAGTTTATAACAAGATTTGGGAATGAACTCAACAATGTTGCCACGATTACTGTTACTGACGGTTGCGAATGGCATATGGAAGTGAAGAAATGTGGCAACGAAGTTTTCTTTTGCAAAAATTGGCAACCGTTTGCAGAACACTACTCTATCTGTTATGGTTGTTATGTAGATTTCAAGTATGAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAAATTCTGTCGAGATTTCTTATCCATTCAAAACTGGGAAGACTAATGGAGAACCAAACCCAAAAAACCCTTCTTTTCGTTCCCAATCCTTTGACTGTCGATATGCGGTGAGGATTTTACTGTACATTATTTGGTTTAAAGTAAATGTTTGAGTTATATATTTATGCGGCCACTTAATTAGATTGTTCCTCCATTGTGTAGTATGTTCCTGCTGAATTCGCCGCAGAATATTTGAAGCCAAATGTTCTGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGAACAAAGGCGGGGGAAAAGCAATGCGAATAACGTTTGGATTCTTTAGATTTGCAAGGGAAAACAATCTATCAGATGGAGTTATGTACGAGTTTGAGCTTATCAAGAGGAAGCCGCGTGTTCTGTTCTAA
Protein sequence

>MS.gene64660.t1

MQPRRRRFVPSPAPEKDSKHFMKTILPPPMHTKEIRIPDEFITRFGNELNNVATITVTDGCEWHMEVKKCGNEVFFCKNWQPFAEHYSICYGCYVDFKYEGNSKFNVVIYDTNSVEISYPFKTGKTNGEPNPKNPSFRSQSFDCRYAYVPAEFAAEYLKPNVLFKLQNSQGKQWEVYCVLNKGGGKAMRITFGFFRFARENNLSDGVMYEFELIKRKPRVLF