Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64905.t1 | XP_013441754.1 | 86.1 | 295 | 40 | 1 | 1 | 294 | 1 | 295 | 5.20E-142 | 513.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64905.t1 | Q9LKR4 | 24.2 | 260 | 167 | 7 | 40 | 287 | 60 | 301 | 1.4e-13 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64905.t1 | A0A072TE13 | 86.1 | 295 | 40 | 1 | 1 | 294 | 1 | 295 | 3.8e-142 | 513.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene64905.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64905.t1 | MTR_0558s0020 | 86.102 | 295 | 40 | 1 | 1 | 294 | 1 | 295 | 0.0 | 531 |
| MS.gene64905.t1 | MTR_1g083640 | 81.690 | 284 | 36 | 1 | 1 | 268 | 1 | 284 | 1.98e-174 | 484 |
| MS.gene64905.t1 | MTR_8g446020 | 72.789 | 294 | 41 | 1 | 1 | 294 | 1 | 255 | 1.92e-154 | 432 |
| MS.gene64905.t1 | MTR_2g014100 | 81.224 | 245 | 37 | 1 | 1 | 236 | 1 | 245 | 4.88e-146 | 410 |
| MS.gene64905.t1 | MTR_6g090505 | 82.251 | 231 | 41 | 0 | 65 | 295 | 1 | 231 | 5.73e-142 | 404 |
| MS.gene64905.t1 | MTR_0337s0040 | 71.648 | 261 | 34 | 2 | 1 | 260 | 1 | 222 | 2.22e-128 | 365 |
| MS.gene64905.t1 | MTR_3g008240 | 84.729 | 203 | 30 | 1 | 1 | 202 | 1 | 203 | 1.71e-125 | 357 |
| MS.gene64905.t1 | MTR_4g040430 | 82.178 | 202 | 32 | 1 | 1 | 202 | 1 | 198 | 1.64e-119 | 341 |
| MS.gene64905.t1 | MTR_5g071590 | 72.778 | 180 | 36 | 2 | 1 | 167 | 1 | 180 | 8.53e-92 | 271 |
| MS.gene64905.t1 | MTR_1g055240 | 86.620 | 142 | 19 | 0 | 91 | 232 | 3 | 144 | 3.57e-90 | 265 |
| MS.gene64905.t1 | MTR_0019s0290 | 80.263 | 152 | 30 | 0 | 82 | 233 | 9 | 160 | 6.23e-88 | 259 |
| MS.gene64905.t1 | MTR_1281s0010 | 79.817 | 109 | 22 | 0 | 19 | 127 | 1 | 109 | 7.01e-59 | 184 |
| MS.gene64905.t1 | MTR_4g008820 | 85.000 | 80 | 11 | 1 | 1 | 79 | 125 | 204 | 5.79e-35 | 134 |
| MS.gene64905.t1 | MTR_8g046060 | 78.409 | 88 | 10 | 2 | 1 | 79 | 91 | 178 | 4.68e-34 | 124 |
| MS.gene64905.t1 | MTR_6g051425 | 31.873 | 251 | 152 | 5 | 40 | 288 | 22 | 255 | 1.65e-30 | 117 |
| MS.gene64905.t1 | MTR_1g070355 | 27.092 | 251 | 164 | 4 | 41 | 291 | 80 | 311 | 2.12e-18 | 85.9 |
| MS.gene64905.t1 | MTR_0337s0050 | 65.116 | 43 | 15 | 0 | 259 | 301 | 14 | 56 | 2.72e-14 | 67.4 |
| MS.gene64905.t1 | MTR_0402s0040 | 24.865 | 185 | 120 | 4 | 49 | 233 | 40 | 205 | 7.04e-14 | 72.0 |
| MS.gene64905.t1 | MTR_4g035820 | 22.727 | 286 | 186 | 8 | 17 | 294 | 61 | 319 | 4.20e-12 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64905.t1 | AT4G38180 | 28.352 | 261 | 169 | 5 | 32 | 292 | 70 | 312 | 2.64e-24 | 103 |
| MS.gene64905.t1 | AT5G28530 | 24.231 | 260 | 167 | 7 | 40 | 287 | 60 | 301 | 2.93e-15 | 76.6 |
| MS.gene64905.t1 | AT5G28530 | 24.231 | 260 | 167 | 7 | 40 | 287 | 60 | 301 | 2.97e-15 | 76.6 |
| MS.gene64905.t1 | AT5G28530 | 24.231 | 260 | 167 | 7 | 40 | 287 | 60 | 301 | 2.97e-15 | 76.6 |
| MS.gene64905.t1 | AT1G10240 | 22.868 | 258 | 175 | 5 | 49 | 301 | 62 | 300 | 1.13e-13 | 71.6 |
| MS.gene64905.t1 | AT1G10240 | 22.868 | 258 | 175 | 5 | 49 | 301 | 62 | 300 | 1.13e-13 | 71.6 |
| MS.gene64905.t1 | AT4G15090 | 26.459 | 257 | 160 | 8 | 50 | 301 | 66 | 298 | 1.57e-11 | 65.5 |
| MS.gene64905.t1 | AT4G15090 | 26.459 | 257 | 160 | 8 | 50 | 301 | 66 | 298 | 1.57e-11 | 65.5 |
| MS.gene64905.t1 | AT4G15090 | 26.459 | 257 | 160 | 8 | 50 | 301 | 66 | 298 | 1.57e-11 | 65.5 |
| MS.gene64905.t1 | AT4G15090 | 26.459 | 257 | 160 | 8 | 50 | 301 | 66 | 298 | 1.57e-11 | 65.5 |
| MS.gene64905.t1 | AT4G15090 | 26.459 | 257 | 160 | 8 | 50 | 301 | 66 | 298 | 1.57e-11 | 65.5 |
Find 41 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 5.3:-14223199 | MS.gene64905:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.339462 | 5.3:+14223149 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 5.3:-14223142 | MS.gene64905:intron |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 5.3:+14223679 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 5.3:+14223918 | None:intergenic |
| GCCGAACAAACAATAAATAT+CGG | 0.406788 | 5.3:-14223150 | MS.gene64905:CDS |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 5.3:-14223908 | MS.gene64905:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 5.3:-14223837 | MS.gene64905:CDS |
| TGAATGTTTATATTGATTGA+AGG | 0.419189 | 5.3:-14222995 | None:intergenic |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 5.3:-14223502 | MS.gene64905:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 5.3:-14223620 | MS.gene64905:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 5.3:-14223588 | MS.gene64905:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 5.3:-14223637 | MS.gene64905:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 5.3:-14223472 | MS.gene64905:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 5.3:-14223983 | MS.gene64905:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 5.3:-14223788 | MS.gene64905:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 5.3:+14223643 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 5.3:-14223850 | MS.gene64905:CDS |
| TCTAATGACGACTCGTCAAA+AGG | 0.488508 | 5.3:-14223359 | MS.gene64905:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 5.3:-14223794 | MS.gene64905:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 5.3:-14223346 | MS.gene64905:CDS |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 5.3:+14223388 | None:intergenic |
| TGTCTTCTACAGCATCAACT+TGG | 0.523056 | 5.3:+14223254 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 5.3:-14223584 | MS.gene64905:CDS |
| AAATGCTCTGCTTTATGTAA+TGG | 0.525118 | 5.3:+14223544 | None:intergenic |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 5.3:-14223743 | MS.gene64905:CDS |
| TGTTATGCTTGTAACAAAGA+AGG | 0.541604 | 5.3:-14223754 | MS.gene64905:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 5.3:-14223918 | MS.gene64905:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 5.3:-14223742 | MS.gene64905:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.551307 | 5.3:-14223440 | MS.gene64905:CDS |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 5.3:-14223292 | MS.gene64905:CDS |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 5.3:-14223959 | MS.gene64905:CDS |
| GAAGTGATGGGTGAAGGTGT+TGG | 0.603704 | 5.3:-14223427 | MS.gene64905:CDS |
| GACGACTCGTCAAAAGGAGA+TGG | 0.604288 | 5.3:-14223353 | MS.gene64905:CDS |
| GACTCGTCAAAAGGAGATGG+TGG | 0.611157 | 5.3:-14223350 | MS.gene64905:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 5.3:-14223994 | MS.gene64905:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 5.3:+14223694 | None:intergenic |
| GTGATGGGTGAAGGTGTTGG+AGG | 0.621481 | 5.3:-14223424 | MS.gene64905:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.643044 | 5.3:-14223439 | MS.gene64905:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 5.3:-14223585 | MS.gene64905:CDS |
| TCTTATGAAGTGATGGGTGA+AGG | 0.656961 | 5.3:-14223433 | MS.gene64905:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | - | chr5.3:14223207-14223226 | MS.gene64905:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | - | chr5.3:14223413-14223432 | MS.gene64905:CDS | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | - | chr5.3:14223151-14223170 | MS.gene64905:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | chr5.3:14223213-14223232 | MS.gene64905:CDS | 25.0% |
| ! | AGTACAAAATACTAACCTAA+TGG | + | chr5.3:14223877-14223896 | None:intergenic | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | - | chr5.3:14223416-14223435 | MS.gene64905:CDS | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | chr5.3:14223042-14223061 | MS.gene64905:intron | 25.0% |
| ! | TGAAATTTGAATGAATGTTC+TGG | - | chr5.3:14223924-14223943 | MS.gene64905:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | - | chr5.3:14223417-14223436 | MS.gene64905:CDS | 25.0% |
| !!! | AAGACATAGCTAGTATTTTT+TGG | - | chr5.3:14223764-14223783 | MS.gene64905:CDS | 25.0% |
| !!! | AGACATAGCTAGTATTTTTT+GGG | - | chr5.3:14223765-14223784 | MS.gene64905:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | chr5.3:14223203-14223222 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr5.3:14223529-14223548 | MS.gene64905:CDS | 25.0% |
| AAATGCTCTGCTTTATGTAA+TGG | + | chr5.3:14223460-14223479 | None:intergenic | 30.0% | |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr5.3:14223259-14223278 | MS.gene64905:CDS | 30.0% | |
| AACAATAAATATCGGCCATT+AGG | - | chr5.3:14223859-14223878 | MS.gene64905:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | - | chr5.3:14223562-14223581 | MS.gene64905:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | chr5.3:14223802-14223821 | MS.gene64905:CDS | 30.0% | |
| CCATGATTCTTTATCACTAA+AGG | + | chr5.3:14223086-14223105 | None:intergenic | 30.0% | |
| CCTTTAGTGATAAAGAATCA+TGG | - | chr5.3:14223083-14223102 | MS.gene64905:intron | 30.0% | |
| CTGGTATATGTGTGTTAATT+TGG | - | chr5.3:14223963-14223982 | MS.gene64905:CDS | 30.0% | |
| GCCGAACAAACAATAAATAT+CGG | - | chr5.3:14223851-14223870 | MS.gene64905:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr5.3:14223855-14223874 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | chr5.3:14223348-14223367 | MS.gene64905:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | - | chr5.3:14223093-14223112 | MS.gene64905:intron | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr5.3:14223258-14223277 | MS.gene64905:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | chr5.3:14223730-14223749 | None:intergenic | 30.0% | |
| TGTTATGCTTGTAACAAAGA+AGG | - | chr5.3:14223247-14223266 | MS.gene64905:CDS | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | chr5.3:14223616-14223635 | None:intergenic | 30.0% | |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | chr5.3:14223381-14223400 | MS.gene64905:CDS | 30.0% |
| ! | GAATCTTCCTTTTCGATTTT+CGG | - | chr5.3:14223606-14223625 | MS.gene64905:CDS | 30.0% |
| !! | ATGTTCTGGTTTAGAATTGA+AGG | - | chr5.3:14223938-14223957 | MS.gene64905:CDS | 30.0% |
| ATAACACTGGATTCTCTGTT+AGG | - | chr5.3:14223164-14223183 | MS.gene64905:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | chr5.3:14223007-14223026 | MS.gene64905:intron | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr5.3:14223499-14223518 | MS.gene64905:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | chr5.3:14223347-14223366 | MS.gene64905:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | chr5.3:14223342-14223361 | MS.gene64905:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | chr5.3:14223561-14223580 | MS.gene64905:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | chr5.3:14223284-14223303 | MS.gene64905:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | chr5.3:14223325-14223344 | None:intergenic | 35.0% | |
| ATCTGAGAGTGGACAATTGA+CGG | - | chr5.3:14223018-14223037 | MS.gene64905:intron | 40.0% | |
| TCTAATGACGACTCGTCAAA+AGG | - | chr5.3:14223642-14223661 | MS.gene64905:CDS | 40.0% | |
| TGTCTTCTACAGCATCAACT+TGG | + | chr5.3:14223750-14223769 | None:intergenic | 40.0% | |
| ! | TGGTTTAGAATTGAAGGCTC+TGG | - | chr5.3:14223944-14223963 | MS.gene64905:CDS | 40.0% |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | chr5.3:14223298-14223317 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTGA+AGG | - | chr5.3:14223568-14223587 | MS.gene64905:CDS | 40.0% |
| !!! | GCTAGTATTTTTTGGGCAGA+TGG | - | chr5.3:14223772-14223791 | MS.gene64905:CDS | 40.0% |
| AATGAAGCTCTCTCGAAACC+AGG | - | chr5.3:14223709-14223728 | MS.gene64905:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | chr5.3:14223310-14223329 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | chr5.3:14223352-14223371 | MS.gene64905:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | chr5.3:14223361-14223380 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr5.3:14223364-14223383 | MS.gene64905:CDS | 45.0% |
| GACGACTCGTCAAAAGGAGA+TGG | - | chr5.3:14223648-14223667 | MS.gene64905:CDS | 50.0% | |
| GACTCGTCAAAAGGAGATGG+TGG | - | chr5.3:14223651-14223670 | MS.gene64905:CDS | 50.0% | |
| !! | GAAGTGATGGGTGAAGGTGT+TGG | - | chr5.3:14223574-14223593 | MS.gene64905:CDS | 50.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | chr5.3:14223655-14223674 | MS.gene64905:CDS | 55.0% |
| !! | GTGATGGGTGAAGGTGTTGG+AGG | - | chr5.3:14223577-14223596 | MS.gene64905:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 14222998 | 14224025 | 14222998 | ID=MS.gene64905 |
| chr5.3 | mRNA | 14222998 | 14224025 | 14222998 | ID=MS.gene64905.t1;Parent=MS.gene64905 |
| chr5.3 | exon | 14223143 | 14224025 | 14223143 | ID=MS.gene64905.t1.exon1;Parent=MS.gene64905.t1 |
| chr5.3 | CDS | 14223143 | 14224025 | 14223143 | ID=cds.MS.gene64905.t1;Parent=MS.gene64905.t1 |
| chr5.3 | exon | 14222998 | 14223020 | 14222998 | ID=MS.gene64905.t1.exon2;Parent=MS.gene64905.t1 |
| chr5.3 | CDS | 14222998 | 14223020 | 14222998 | ID=cds.MS.gene64905.t1;Parent=MS.gene64905.t1 |
| Gene Sequence |
| Protein sequence |
