AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65055

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65055.t1 MTR_7g057230 89.035 228 25 0 1 228 430 657 2.93e-144 417
MS.gene65055.t1 MTR_6g047750 55.508 236 90 3 2 227 431 661 1.69e-82 259
MS.gene65055.t1 MTR_3g089055 46.262 214 113 2 16 227 351 564 3.43e-60 198
MS.gene65055.t1 MTR_3g089055 46.262 214 113 2 16 227 356 569 4.26e-60 198
MS.gene65055.t1 MTR_1g029420 46.445 211 111 2 19 227 381 591 4.90e-56 187
MS.gene65055.t1 MTR_4g133660 42.991 214 120 2 16 227 329 542 4.89e-52 176
MS.gene65055.t1 MTR_2g082090 41.667 216 124 2 14 227 363 578 3.18e-49 169
MS.gene65055.t1 MTR_3g056110 38.222 225 135 3 6 227 318 541 1.19e-45 159
MS.gene65055.t1 MTR_5g097480 41.546 207 119 2 23 227 337 543 1.29e-45 159
MS.gene65055.t1 MTR_2g026250 39.336 211 126 2 19 227 385 595 2.21e-45 159
MS.gene65055.t1 MTR_5g094450 39.906 213 123 4 19 227 320 531 9.91e-44 154
MS.gene65055.t1 MTR_4g074310 66.667 90 17 2 1 90 114 190 8.52e-30 110
MS.gene65055.t1 MTR_7g027190 31.915 235 139 7 1 227 451 672 5.03e-26 105
MS.gene65055.t1 MTR_8g442410 31.304 230 151 4 2 227 298 524 1.73e-24 101
MS.gene65055.t1 MTR_4g102790 31.718 227 145 7 9 227 437 661 6.09e-24 99.8
MS.gene65055.t1 MTR_3g065980 30.508 236 142 5 10 227 308 539 1.06e-23 99.0
MS.gene65055.t1 MTR_4g104020 27.826 230 155 5 2 227 297 519 7.15e-23 96.7
MS.gene65055.t1 MTR_4g064160 26.066 211 153 2 20 227 473 683 1.93e-19 87.0
MS.gene65055.t1 MTR_4g064200 26.415 212 152 3 20 227 438 649 1.18e-18 84.7
MS.gene65055.t1 MTR_7g062120 25.581 215 149 3 20 227 529 739 1.53e-18 84.3
MS.gene65055.t1 MTR_2g097410 24.528 212 156 3 20 227 529 740 1.68e-18 84.3
MS.gene65055.t1 MTR_7g109580 25.541 231 164 3 1 227 327 553 1.74e-18 84.0
MS.gene65055.t1 MTR_7g074650 28.899 218 142 7 14 227 583 791 2.02e-18 84.0
MS.gene65055.t1 MTR_2g097463 26.540 211 152 3 20 227 444 654 2.98e-18 83.6
MS.gene65055.t1 MTR_4g097080 25.424 236 162 4 2 227 271 502 3.22e-18 83.2
MS.gene65055.t1 MTR_4g076020 27.928 222 146 8 11 227 216 428 3.94e-18 82.8
MS.gene65055.t1 MTR_2g097473 26.066 211 153 3 20 227 443 653 5.64e-18 82.4
MS.gene65055.t1 MTR_2g097467 25.352 213 152 4 20 227 443 653 7.33e-17 79.3
MS.gene65055.t1 MTR_2g097350 25.541 231 167 3 2 227 409 639 1.24e-16 78.6
MS.gene65055.t1 MTR_2g097310 25.000 236 164 4 2 228 407 638 5.86e-16 76.6
MS.gene65055.t1 MTR_4g064150 22.642 212 160 3 20 227 521 732 6.45e-16 76.6
MS.gene65055.t1 MTR_2g097390 23.810 231 171 3 2 227 410 640 9.79e-16 75.9
MS.gene65055.t1 MTR_2g034250 27.660 235 158 6 1 227 354 584 3.71e-15 74.3
MS.gene65055.t1 MTR_5g015950 24.906 265 161 5 1 227 181 445 6.41e-15 73.6
MS.gene65055.t1 MTR_3g053270 24.906 265 161 5 1 227 181 445 6.41e-15 73.6
MS.gene65055.t1 MTR_2g034260 27.778 234 158 6 1 227 354 583 6.48e-15 73.6
MS.gene65055.t1 MTR_2g097380 25.862 232 150 6 2 227 345 560 6.96e-15 73.6
MS.gene65055.t1 MTR_4g064180 24.286 210 155 3 20 225 414 623 7.50e-15 73.6
MS.gene65055.t1 MTR_5g009080 32.468 154 91 4 82 227 329 477 2.42e-14 71.6
MS.gene65055.t1 MTR_4g064120 23.333 210 157 3 20 225 414 623 5.30e-14 70.9
MS.gene65055.t1 MTR_4g076140 31.333 150 98 3 82 227 320 468 5.66e-14 70.9
MS.gene65055.t1 MTR_4g077760 31.776 214 132 7 20 227 349 554 6.32e-14 70.9
MS.gene65055.t1 MTR_3g022830 30.488 164 107 4 71 227 272 435 8.18e-14 70.1
MS.gene65055.t1 MTR_2g034280 26.271 236 159 7 1 227 345 574 1.23e-13 69.7
MS.gene65055.t1 MTR_1g069725 28.372 215 138 7 20 227 257 462 3.98e-13 68.2
MS.gene65055.t1 MTR_4g095500 22.727 242 157 3 16 227 226 467 1.07e-12 67.0
MS.gene65055.t1 MTR_5g015490 24.370 238 168 5 2 227 252 489 3.40e-12 65.5
MS.gene65055.t1 MTR_1g086970 24.675 231 165 4 1 227 252 477 5.69e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65055.t1 AT5G52510 54.167 240 94 4 1 227 403 639 7.59e-81 254
MS.gene65055.t1 AT1G21450 42.326 215 121 2 16 227 378 592 5.59e-50 172
MS.gene65055.t1 AT5G48150 43.128 211 118 2 19 227 279 489 6.30e-48 164
MS.gene65055.t1 AT5G48150 43.128 211 118 2 19 227 279 489 6.30e-48 164
MS.gene65055.t1 AT5G48150 43.128 211 118 2 19 227 279 489 6.30e-48 164
MS.gene65055.t1 AT5G48150 43.128 211 118 2 19 227 279 489 6.30e-48 164
MS.gene65055.t1 AT2G04890 38.596 228 135 3 2 227 188 412 1.17e-47 162
MS.gene65055.t1 AT1G50600 41.121 214 124 2 16 227 312 525 4.12e-47 163
MS.gene65055.t1 AT1G50600 41.706 211 121 2 19 227 386 596 8.25e-47 163
MS.gene65055.t1 AT1G50600 41.706 211 121 2 19 227 390 600 9.20e-47 163
MS.gene65055.t1 AT4G17230 38.498 213 125 3 19 227 314 524 1.98e-39 142
MS.gene65055.t1 AT4G17230 38.498 213 125 3 19 227 314 524 1.98e-39 142
MS.gene65055.t1 AT1G66350 31.651 218 137 5 15 227 295 505 3.30e-27 108
MS.gene65055.t1 AT3G03450 33.484 221 138 4 12 227 328 544 3.67e-27 108
MS.gene65055.t1 AT5G17490 30.043 233 150 6 2 227 289 515 3.67e-23 97.4
MS.gene65055.t1 AT2G29065 27.907 215 149 4 20 228 414 628 5.28e-23 97.1
MS.gene65055.t1 AT3G50650 32.227 211 130 4 27 227 334 541 6.11e-21 91.3
MS.gene65055.t1 AT3G46600 26.291 213 150 3 20 227 160 370 7.68e-21 90.1
MS.gene65055.t1 AT1G14920 30.180 222 140 6 15 227 313 528 8.51e-21 90.9
MS.gene65055.t1 AT2G01570 30.631 222 142 5 12 227 365 580 2.51e-20 89.4
MS.gene65055.t1 AT3G46600 26.291 213 150 3 20 227 336 546 3.75e-20 89.0
MS.gene65055.t1 AT3G46600 26.291 213 150 3 20 227 368 578 4.74e-20 88.6
MS.gene65055.t1 AT2G29060 25.000 212 155 2 20 227 480 691 3.21e-19 86.3
MS.gene65055.t1 AT2G29060 25.000 212 155 2 20 227 443 654 3.40e-19 86.3
MS.gene65055.t1 AT5G66770 30.622 209 132 5 27 227 380 583 5.20e-19 85.5
MS.gene65055.t1 AT1G07530 24.645 211 156 2 20 227 554 764 9.76e-19 85.1
MS.gene65055.t1 AT5G59450 25.352 213 154 4 20 227 385 597 1.71e-18 84.0
MS.gene65055.t1 AT2G37650 25.701 214 150 3 20 227 490 700 5.44e-18 82.8
MS.gene65055.t1 AT2G37650 25.701 214 150 3 20 227 502 712 6.42e-18 82.4
MS.gene65055.t1 AT3G54220 27.778 216 143 7 16 227 443 649 1.18e-17 81.6
MS.gene65055.t1 AT4G37650 27.778 252 147 8 2 227 286 528 1.38e-16 78.6
MS.gene65055.t1 AT1G63100 31.429 175 116 4 20 192 407 579 2.92e-15 74.7
MS.gene65055.t1 AT1G63100 31.429 175 116 4 20 192 432 604 3.13e-15 74.7
MS.gene65055.t1 AT1G07520 22.791 215 160 4 20 228 315 529 3.21e-15 74.3
MS.gene65055.t1 AT1G07520 22.791 215 160 4 20 228 315 529 3.21e-15 74.3
MS.gene65055.t1 AT1G07520 22.791 215 160 4 20 228 479 693 4.30e-15 74.3
MS.gene65055.t1 AT1G07520 22.791 215 160 4 20 228 480 694 4.34e-15 74.3
MS.gene65055.t1 AT1G50420 30.667 150 99 3 82 227 330 478 1.37e-14 72.8
MS.gene65055.t1 AT3G49950 26.404 178 123 3 56 228 234 408 2.14e-12 65.9
MS.gene65055.t1 AT5G41920 27.064 218 137 9 18 227 196 399 4.39e-12 65.1

Find 42 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCACTGTTGCATCTTCT+TGG 0.219398 7.1:+43956368 MS.gene65055:CDS
TAAAACCATTGGAGAACTTT+TGG 0.220013 7.1:+43955757 MS.gene65055:CDS
CCCAAGCATTGTCACAGTTT+TGG 0.223353 7.1:+43955985 MS.gene65055:CDS
GAGAGATTAAAGCTTGAAAA+AGG 0.266629 7.1:+43956121 MS.gene65055:CDS
GAGTTAGTTCGGTGAGATTT+AGG 0.277013 7.1:-43955829 None:intergenic
CGTGCACGCATGAGCATGGC+TGG 0.308371 7.1:+43956223 MS.gene65055:CDS
GGTAGAGAAGCTGAGAGTTT+AGG 0.329057 7.1:+43955779 MS.gene65055:CDS
TGGAGTTTGCTTTGGTTGGA+TGG 0.363970 7.1:+43956339 MS.gene65055:CDS
TCCGTTGACACGCTCTCGTC+TGG 0.391830 7.1:-43955920 None:intergenic
CAATGACTCGCGAGTTAGTT+CGG 0.393659 7.1:-43955840 None:intergenic
ATGGTGGAGTTTGCTTTGGT+TGG 0.406060 7.1:+43956335 MS.gene65055:CDS
CCAAAACTGTGACAATGCTT+GGG 0.425816 7.1:-43955985 None:intergenic
GGAGTTTGCTTTGGTTGGAT+GGG 0.429128 7.1:+43956340 MS.gene65055:CDS
CAGTAACTTTCCGACTCATC+AGG 0.431407 7.1:-43956258 None:intergenic
AAGCTGAGAGTTTAGGAATC+GGG 0.432375 7.1:+43955786 MS.gene65055:CDS
GCACAGTAATTCTGCTACCT+TGG 0.435103 7.1:-43956303 None:intergenic
TCCAAAACTGTGACAATGCT+TGG 0.439492 7.1:-43955986 None:intergenic
CCATTAGTTTCACGCGTGGC+TGG 0.468708 7.1:+43956034 MS.gene65055:CDS
GAGAATGGTGGAGTTTGCTT+TGG 0.469489 7.1:+43956331 MS.gene65055:CDS
TCGGTGAGATTTAGGTTCAC+TGG 0.483877 7.1:-43955821 None:intergenic
GAAGCTGAGAGTTTAGGAAT+CGG 0.488612 7.1:+43955785 MS.gene65055:CDS
CCAGCCACGCGTGAAACTAA+TGG 0.503031 7.1:-43956034 None:intergenic
TCTACCCAAAAGTTCTCCAA+TGG 0.528471 7.1:-43955762 None:intergenic
GTGGCGTGCACGCATGAGCA+TGG 0.530408 7.1:+43956219 MS.gene65055:CDS
ACCAGACGAGAGCGTGTCAA+CGG 0.534077 7.1:+43955919 MS.gene65055:CDS
AAGGGAGGGATCGTGTGGGA+AGG 0.540426 7.1:+43956179 MS.gene65055:CDS
TGCAGTTGCTTGTGAAGGGA+GGG 0.563266 7.1:+43956165 MS.gene65055:CDS
TTAGATTAAACCTGATGAGT+CGG 0.580861 7.1:+43956248 MS.gene65055:CDS
GTGCACGCATGAGCATGGCT+GGG 0.597641 7.1:+43956224 MS.gene65055:CDS
TTGTGAAGGGAGGGATCGTG+TGG 0.600325 7.1:+43956174 MS.gene65055:CDS
AGAAATGCAGTTGCTTGTGA+AGG 0.600882 7.1:+43956160 MS.gene65055:CDS
ATGCAGTTGCTTGTGAAGGG+AGG 0.602267 7.1:+43956164 MS.gene65055:CDS
ATTACTGTGCATGAAGAGAA+TGG 0.610201 7.1:+43956316 MS.gene65055:CDS
CAAAACTGTGACAATGCTTG+GGG 0.612898 7.1:-43955984 None:intergenic
AGAATCTATTGAATTCGCGA+TGG 0.614659 7.1:+43956084 MS.gene65055:CDS
TGACGAAGAAGCGTGTCACG+TGG 0.628901 7.1:-43955947 None:intergenic
AGCACCATTAGTTTCACGCG+TGG 0.655950 7.1:+43956030 MS.gene65055:CDS
GAAATGCAGTTGCTTGTGAA+GGG 0.657022 7.1:+43956161 MS.gene65055:CDS
ATAACTTCAAGACTCATCCA+AGG 0.671136 7.1:+43956286 MS.gene65055:CDS
TGTGAAGGGAGGGATCGTGT+GGG 0.691594 7.1:+43956175 MS.gene65055:CDS
GTTGAAGATCACGACTGTGA+CGG 0.707788 7.1:+43955712 MS.gene65055:CDS
ACTGTGCATGAAGAGAATGG+TGG 0.760237 7.1:+43956319 MS.gene65055:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAAGAATTTTAAAACCAT+TGG + chr7.1:43955746-43955765 MS.gene65055:CDS 20.0%
GAGAGATTAAAGCTTGAAAA+AGG + chr7.1:43956121-43956140 MS.gene65055:CDS 30.0%
TTAGATTAAACCTGATGAGT+CGG + chr7.1:43956248-43956267 MS.gene65055:CDS 30.0%
! AAAACCATTGGAGAACTTTT+GGG + chr7.1:43955758-43955777 MS.gene65055:CDS 30.0%
! TAAAACCATTGGAGAACTTT+TGG + chr7.1:43955757-43955776 MS.gene65055:CDS 30.0%
AGAATCTATTGAATTCGCGA+TGG + chr7.1:43956084-43956103 MS.gene65055:CDS 35.0%
ATAACTTCAAGACTCATCCA+AGG + chr7.1:43956286-43956305 MS.gene65055:CDS 35.0%
ATTACTGTGCATGAAGAGAA+TGG + chr7.1:43956316-43956335 MS.gene65055:CDS 35.0%
AAGCTGAGAGTTTAGGAATC+GGG + chr7.1:43955786-43955805 MS.gene65055:CDS 40.0%
AGAAATGCAGTTGCTTGTGA+AGG + chr7.1:43956160-43956179 MS.gene65055:CDS 40.0%
CAAAACTGTGACAATGCTTG+GGG - chr7.1:43955987-43956006 None:intergenic 40.0%
CCAAAACTGTGACAATGCTT+GGG - chr7.1:43955988-43956007 None:intergenic 40.0%
GAAATGCAGTTGCTTGTGAA+GGG + chr7.1:43956161-43956180 MS.gene65055:CDS 40.0%
GAAGCTGAGAGTTTAGGAAT+CGG + chr7.1:43955785-43955804 MS.gene65055:CDS 40.0%
TCCAAAACTGTGACAATGCT+TGG - chr7.1:43955989-43956008 None:intergenic 40.0%
TCTACCCAAAAGTTCTCCAA+TGG - chr7.1:43955765-43955784 None:intergenic 40.0%
! GAGTTAGTTCGGTGAGATTT+AGG - chr7.1:43955832-43955851 None:intergenic 40.0%
ACTGTGCATGAAGAGAATGG+TGG + chr7.1:43956319-43956338 MS.gene65055:CDS 45.0%
CAATGACTCGCGAGTTAGTT+CGG - chr7.1:43955843-43955862 None:intergenic 45.0%
CAGTAACTTTCCGACTCATC+AGG - chr7.1:43956261-43956280 None:intergenic 45.0%
CTCTCACTGTTGCATCTTCT+TGG + chr7.1:43956368-43956387 MS.gene65055:CDS 45.0%
GCACAGTAATTCTGCTACCT+TGG - chr7.1:43956306-43956325 None:intergenic 45.0%
GGTAGAGAAGCTGAGAGTTT+AGG + chr7.1:43955779-43955798 MS.gene65055:CDS 45.0%
GTTGAAGATCACGACTGTGA+CGG + chr7.1:43955712-43955731 MS.gene65055:CDS 45.0%
! ATGGTGGAGTTTGCTTTGGT+TGG + chr7.1:43956335-43956354 MS.gene65055:CDS 45.0%
! GAGAATGGTGGAGTTTGCTT+TGG + chr7.1:43956331-43956350 MS.gene65055:CDS 45.0%
! GGAGTTTGCTTTGGTTGGAT+GGG + chr7.1:43956340-43956359 MS.gene65055:CDS 45.0%
! TCGGTGAGATTTAGGTTCAC+TGG - chr7.1:43955824-43955843 None:intergenic 45.0%
! TGGAGTTTGCTTTGGTTGGA+TGG + chr7.1:43956339-43956358 MS.gene65055:CDS 45.0%
!! CCCAAGCATTGTCACAGTTT+TGG + chr7.1:43955985-43956004 MS.gene65055:CDS 45.0%
!! GTGGGAAGGTGTGAAGTTTT+TGG + chr7.1:43956193-43956212 MS.gene65055:CDS 45.0%
!!! GGTGTGAAGTTTTTGGCAAG+TGG + chr7.1:43956200-43956219 MS.gene65055:CDS 45.0%
ATGCAGTTGCTTGTGAAGGG+AGG + chr7.1:43956164-43956183 MS.gene65055:CDS 50.0%
TGCAGTTGCTTGTGAAGGGA+GGG + chr7.1:43956165-43956184 MS.gene65055:CDS 50.0%
! AGCACCATTAGTTTCACGCG+TGG + chr7.1:43956030-43956049 MS.gene65055:CDS 50.0%
CCAGCCACGCGTGAAACTAA+TGG - chr7.1:43956037-43956056 None:intergenic 55.0%
TGTGAAGGGAGGGATCGTGT+GGG + chr7.1:43956175-43956194 MS.gene65055:CDS 55.0%
TTGTGAAGGGAGGGATCGTG+TGG + chr7.1:43956174-43956193 MS.gene65055:CDS 55.0%
! ACCAGACGAGAGCGTGTCAA+CGG + chr7.1:43955919-43955938 MS.gene65055:CDS 55.0%
! CCATTAGTTTCACGCGTGGC+TGG + chr7.1:43956034-43956053 MS.gene65055:CDS 55.0%
! TGACGAAGAAGCGTGTCACG+TGG - chr7.1:43955950-43955969 None:intergenic 55.0%
AAGGGAGGGATCGTGTGGGA+AGG + chr7.1:43956179-43956198 MS.gene65055:CDS 60.0%
GTGCACGCATGAGCATGGCT+GGG + chr7.1:43956224-43956243 MS.gene65055:CDS 60.0%
TCCGTTGACACGCTCTCGTC+TGG - chr7.1:43955923-43955942 None:intergenic 60.0%
CGTGCACGCATGAGCATGGC+TGG + chr7.1:43956223-43956242 MS.gene65055:CDS 65.0%
GTGGCGTGCACGCATGAGCA+TGG + chr7.1:43956219-43956238 MS.gene65055:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 43955710 43956396 43955710 ID=MS.gene65055
chr7.1 mRNA 43955710 43956396 43955710 ID=MS.gene65055.t1;Parent=MS.gene65055
chr7.1 exon 43955710 43956396 43955710 ID=MS.gene65055.t1.exon1;Parent=MS.gene65055.t1
chr7.1 CDS 43955710 43956396 43955710 ID=cds.MS.gene65055.t1;Parent=MS.gene65055.t1
Gene Sequence

>MS.gene65055

ATGTTGAAGATCACGACTGTGACGGTGAACGTCGACAACAAGAATTTTAAAACCATTGGAGAACTTTTGGGTAGAGAAGCTGAGAGTTTAGGAATCGGGTTTGAATTCAAACCAGTGAACCTAAATCTCACCGAACTAACTCGCGAGTCATTGAATTGTGACTCAGACGATATTCTTGCTGTGAATTTTGCGTTCAATCTATACAAAATACCAGACGAGAGCGTGTCAACGGAGAATCCACGTGACACGCTTCTTCGTCAAGTGAAATCACTCTCCCCAAGCATTGTCACAGTTTTGGAGCAAGAGATGAATACAAACACAGCACCATTAGTTTCACGCGTGGCTGGAACTTGTTCTTATTACAACACATTGCTAGAATCTATTGAATTCGCGATGGATCATGATAGTTATGAGAGATTAAAGCTTGAAAAAGGACTGAGCATGAAGATGAGAAATGCAGTTGCTTGTGAAGGGAGGGATCGTGTGGGAAGGTGTGAAGTTTTTGGCAAGTGGCGTGCACGCATGAGCATGGCTGGGTTTAGATTAAACCTGATGAGTCGGAAAGTTACTGAGTCGATAACTTCAAGACTCATCCAAGGTAGCAGAATTACTGTGCATGAAGAGAATGGTGGAGTTTGCTTTGGTTGGATGGGAAGAACTCTCACTGTTGCATCTTCTTGGTGTTAA
Protein sequence

>MS.gene65055.t1

MLKITTVTVNVDNKNFKTIGELLGREAESLGIGFEFKPVNLNLTELTRESLNCDSDDILAVNFAFNLYKIPDESVSTENPRDTLLRQVKSLSPSIVTVLEQEMNTNTAPLVSRVAGTCSYYNTLLESIEFAMDHDSYERLKLEKGLSMKMRNAVACEGRDRVGRCEVFGKWRARMSMAGFRLNLMSRKVTESITSRLIQGSRITVHEENGGVCFGWMGRTLTVASSWC