Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65056.t1 | XP_003622913.2 | 92.7 | 123 | 9 | 0 | 1 | 123 | 296 | 418 | 8.70E-58 | 233 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65056.t1 | Q9FYR7 | 46.3 | 123 | 60 | 2 | 1 | 123 | 270 | 386 | 6.2e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65056.t1 | G7L175 | 92.7 | 123 | 9 | 0 | 1 | 123 | 296 | 418 | 6.3e-58 | 233.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene65056.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65056.t1 | MTR_7g057230 | 92.683 | 123 | 9 | 0 | 1 | 123 | 296 | 418 | 2.21e-77 | 242 |
| MS.gene65056.t1 | MTR_4g074320 | 84.553 | 123 | 19 | 0 | 1 | 123 | 58 | 180 | 1.51e-74 | 221 |
| MS.gene65056.t1 | MTR_4g074310 | 76.991 | 113 | 26 | 0 | 1 | 113 | 1 | 113 | 2.13e-61 | 188 |
| MS.gene65056.t1 | MTR_6g047750 | 56.800 | 125 | 50 | 2 | 1 | 124 | 297 | 418 | 4.25e-38 | 136 |
| MS.gene65056.t1 | MTR_3g089055 | 33.333 | 126 | 75 | 3 | 2 | 123 | 201 | 321 | 2.10e-12 | 63.5 |
| MS.gene65056.t1 | MTR_3g089055 | 33.333 | 126 | 75 | 3 | 2 | 123 | 206 | 326 | 2.15e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65056.t1 | AT5G52510 | 46.341 | 123 | 60 | 2 | 1 | 123 | 270 | 386 | 9.08e-26 | 102 |
| MS.gene65056.t1 | AT1G21450 | 31.200 | 125 | 77 | 2 | 4 | 124 | 230 | 349 | 2.59e-11 | 60.5 |
Find 24 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATTTCTGAAGGCAAAA+TGG | 0.230620 | 7.1:+43954714 | MS.gene65056:CDS |
| TTGCTTCAAGATCTCTTTCA+TGG | 0.245962 | 7.1:+43954930 | MS.gene65056:CDS |
| GACTTCAAGGATTGCGATAT+TGG | 0.275042 | 7.1:-43954956 | None:intergenic |
| TCACACTCTTTGTTGTATCT+TGG | 0.314011 | 7.1:-43954984 | None:intergenic |
| TGGCTGAACAATTCCGCAAC+TGG | 0.355488 | 7.1:-43954865 | None:intergenic |
| AAAATTTGATAGTTGGTTAG+TGG | 0.360184 | 7.1:+43954789 | MS.gene65056:CDS |
| GAGTTGACTCGGCATGTTCT+TGG | 0.375716 | 7.1:-43954885 | None:intergenic |
| CAAAACTAAAATTTGATAGT+TGG | 0.391003 | 7.1:+43954782 | MS.gene65056:CDS |
| TATCTTGGAATGCGACTTCA+AGG | 0.416127 | 7.1:-43954969 | None:intergenic |
| GGATGCAAGTGTATCACATT+GGG | 0.422500 | 7.1:+43955077 | MS.gene65056:CDS |
| CACTTGCATCCGTGCCTGCT+TGG | 0.425624 | 7.1:-43955065 | None:intergenic |
| AACAATTCCGCAACTGGAGG+TGG | 0.434762 | 7.1:-43954859 | None:intergenic |
| GTGTATGTGAGAAACTAGAT+AGG | 0.478094 | 7.1:-43954753 | None:intergenic |
| CGGATGCAAGTGTATCACAT+TGG | 0.523324 | 7.1:+43955076 | MS.gene65056:CDS |
| TACATCAATCTGCTCCAAGC+AGG | 0.561232 | 7.1:+43955051 | MS.gene65056:CDS |
| CAATCTGCTCCAAGCAGGCA+CGG | 0.582308 | 7.1:+43955056 | MS.gene65056:CDS |
| GCTGCCACTGCAATTTCTGA+AGG | 0.584449 | 7.1:+43954706 | MS.gene65056:CDS |
| TGAAAATCCACCTCCAGTTG+CGG | 0.610781 | 7.1:+43954852 | MS.gene65056:CDS |
| TGTGAAGAATCTCTGTGTTG+TGG | 0.612679 | 7.1:+43955002 | MS.gene65056:CDS |
| AAAGAGAAGTTGAGTTGACT+CGG | 0.617772 | 7.1:-43954896 | None:intergenic |
| AGGCAAAATGGATTATGTGA+AGG | 0.626075 | 7.1:+43954726 | MS.gene65056:CDS |
| CTGAACAATTCCGCAACTGG+AGG | 0.643441 | 7.1:-43954862 | None:intergenic |
| TTTGCCTTCAGAAATTGCAG+TGG | 0.681099 | 7.1:-43954710 | None:intergenic |
| GATGCAAGTGTATCACATTG+GGG | 0.686887 | 7.1:+43955078 | MS.gene65056:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTATCAAATTTTAGTTTT+GGG | - | chr7.1:43954783-43954802 | None:intergenic | 15.0% |
| !!! | CAAAACTAAAATTTGATAGT+TGG | + | chr7.1:43954782-43954801 | MS.gene65056:CDS | 20.0% |
| !!! | CAACTATCAAATTTTAGTTT+TGG | - | chr7.1:43954784-43954803 | None:intergenic | 20.0% |
| ! | ATTGCTTGAAATAAAAAGCA+AGG | - | chr7.1:43955119-43955138 | None:intergenic | 25.0% |
| !! | AAAATTTGATAGTTGGTTAG+TGG | + | chr7.1:43954789-43954808 | MS.gene65056:CDS | 25.0% |
| TGAAATAAAAAGCAAGGAGT+GGG | - | chr7.1:43955113-43955132 | None:intergenic | 30.0% | |
| TTGAAATAAAAAGCAAGGAG+TGG | - | chr7.1:43955114-43955133 | None:intergenic | 30.0% | |
| !! | GTGGATTTTGATATTGGTAA+TGG | + | chr7.1:43955021-43955040 | MS.gene65056:CDS | 30.0% |
| !! | TGTGTTGTGGATTTTGATAT+TGG | + | chr7.1:43955015-43955034 | MS.gene65056:CDS | 30.0% |
| GAAATAAAAAGCAAGGAGTG+GGG | - | chr7.1:43955112-43955131 | None:intergenic | 35.0% | |
| GTGTATGTGAGAAACTAGAT+AGG | - | chr7.1:43954756-43954775 | None:intergenic | 35.0% | |
| TTGCTTCAAGATCTCTTTCA+TGG | + | chr7.1:43954930-43954949 | MS.gene65056:CDS | 35.0% | |
| ! | TCACACTCTTTGTTGTATCT+TGG | - | chr7.1:43954987-43955006 | None:intergenic | 35.0% |
| ! | TGCAATTTCTGAAGGCAAAA+TGG | + | chr7.1:43954714-43954733 | MS.gene65056:CDS | 35.0% |
| !! | AAAGAGAAGTTGAGTTGACT+CGG | - | chr7.1:43954899-43954918 | None:intergenic | 35.0% |
| !! | AGGCAAAATGGATTATGTGA+AGG | + | chr7.1:43954726-43954745 | MS.gene65056:CDS | 35.0% |
| GACTTCAAGGATTGCGATAT+TGG | - | chr7.1:43954959-43954978 | None:intergenic | 40.0% | |
| GATGCAAGTGTATCACATTG+GGG | + | chr7.1:43955078-43955097 | MS.gene65056:CDS | 40.0% | |
| GGATGCAAGTGTATCACATT+GGG | + | chr7.1:43955077-43955096 | MS.gene65056:CDS | 40.0% | |
| TATCTTGGAATGCGACTTCA+AGG | - | chr7.1:43954972-43954991 | None:intergenic | 40.0% | |
| TGTGAAGAATCTCTGTGTTG+TGG | + | chr7.1:43955002-43955021 | MS.gene65056:CDS | 40.0% | |
| TTTGCCTTCAGAAATTGCAG+TGG | - | chr7.1:43954713-43954732 | None:intergenic | 40.0% | |
| CGGATGCAAGTGTATCACAT+TGG | + | chr7.1:43955076-43955095 | MS.gene65056:CDS | 45.0% | |
| TACATCAATCTGCTCCAAGC+AGG | + | chr7.1:43955051-43955070 | MS.gene65056:CDS | 45.0% | |
| TGAAAATCCACCTCCAGTTG+CGG | + | chr7.1:43954852-43954871 | MS.gene65056:CDS | 45.0% | |
| AACAATTCCGCAACTGGAGG+TGG | - | chr7.1:43954862-43954881 | None:intergenic | 50.0% | |
| CTGAACAATTCCGCAACTGG+AGG | - | chr7.1:43954865-43954884 | None:intergenic | 50.0% | |
| GCTGCCACTGCAATTTCTGA+AGG | + | chr7.1:43954706-43954725 | MS.gene65056:CDS | 50.0% | |
| TGGCTGAACAATTCCGCAAC+TGG | - | chr7.1:43954868-43954887 | None:intergenic | 50.0% | |
| !! | GAGTTGACTCGGCATGTTCT+TGG | - | chr7.1:43954888-43954907 | None:intergenic | 50.0% |
| CAATCTGCTCCAAGCAGGCA+CGG | + | chr7.1:43955056-43955075 | MS.gene65056:CDS | 55.0% | |
| CACTTGCATCCGTGCCTGCT+TGG | - | chr7.1:43955068-43955087 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 43954700 | 43955143 | 43954700 | ID=MS.gene65056 |
| chr7.1 | mRNA | 43954700 | 43955143 | 43954700 | ID=MS.gene65056.t1;Parent=MS.gene65056 |
| chr7.1 | exon | 43954700 | 43955143 | 43954700 | ID=MS.gene65056.t1.exon1;Parent=MS.gene65056.t1 |
| chr7.1 | CDS | 43954700 | 43955143 | 43954700 | ID=cds.MS.gene65056.t1;Parent=MS.gene65056.t1 |
| Gene Sequence |
| Protein sequence |
