AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65148

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65148.t1 MTR_7g059400 92.068 353 23 2 1 353 1 348 0.0 552
MS.gene65148.t1 MTR_4g022370 48.303 383 125 16 1 340 1 353 2.51e-88 270
MS.gene65148.t1 MTR_4g063780 43.266 349 160 12 1 329 1 331 8.28e-72 227
MS.gene65148.t1 MTR_2g096740 40.357 280 107 10 1 260 1 240 3.56e-50 170
MS.gene65148.t1 MTR_7g024670 55.479 146 57 4 1 142 1 142 1.46e-44 157
MS.gene65148.t1 MTR_2g096740 40.076 262 118 11 1 260 1 225 3.20e-44 154
MS.gene65148.t1 MTR_8g479350 68.421 76 23 1 77 152 69 143 1.98e-31 121
MS.gene65148.t1 MTR_3g090430 63.750 80 29 0 57 136 32 111 1.40e-30 119
MS.gene65148.t1 MTR_3g091820 66.667 75 21 1 57 131 28 98 7.03e-30 116
MS.gene65148.t1 MTR_5g031440 59.633 109 39 1 73 181 40 143 1.36e-29 116
MS.gene65148.t1 MTR_3g077750 63.636 88 25 3 43 126 27 111 1.56e-29 116
MS.gene65148.t1 MTR_2g093220 78.689 61 13 0 70 130 34 94 1.76e-29 115
MS.gene65148.t1 MTR_8g068210 73.438 64 17 0 72 135 28 91 2.68e-29 115
MS.gene65148.t1 MTR_8g079060 60.377 106 25 4 55 152 17 113 2.70e-29 113
MS.gene65148.t1 MTR_4g461080 81.356 59 11 0 70 128 23 81 3.50e-29 114
MS.gene65148.t1 MTR_2g014060 67.606 71 22 1 58 127 6 76 1.79e-28 113
MS.gene65148.t1 MTR_4g109980 90.000 50 5 0 77 126 41 90 5.71e-28 111
MS.gene65148.t1 MTR_8g015840 73.913 69 15 2 68 136 13 78 6.45e-28 108
MS.gene65148.t1 MTR_8g027295 71.014 69 16 1 78 142 26 94 7.68e-28 110
MS.gene65148.t1 MTR_1g056810 80.357 56 11 0 75 130 11 66 7.91e-28 110
MS.gene65148.t1 MTR_2g014170 89.583 48 5 0 77 124 46 93 1.25e-26 107
MS.gene65148.t1 MTR_2g013370 65.278 72 16 2 69 132 31 101 2.09e-26 106
MS.gene65148.t1 MTR_4g089095 81.818 55 9 1 78 132 52 105 2.54e-26 106
MS.gene65148.t1 MTR_4g089095 81.818 55 9 1 78 132 18 71 2.67e-26 105
MS.gene65148.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 4.02e-26 105
MS.gene65148.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 5.56e-26 105
MS.gene65148.t1 MTR_2g059540 87.500 48 6 0 77 124 32 79 1.24e-25 102
MS.gene65148.t1 MTR_6g012450 71.429 56 16 0 78 133 138 193 3.48e-23 100
MS.gene65148.t1 MTR_3g435480 68.421 57 18 0 78 134 138 194 5.76e-23 99.8
MS.gene65148.t1 MTR_7g010950 61.194 67 26 0 78 144 137 203 7.27e-23 99.8
MS.gene65148.t1 MTR_8g044220 68.519 54 17 0 78 131 120 173 9.29e-23 99.0
MS.gene65148.t1 MTR_2g016030 53.247 77 33 1 78 154 49 122 1.03e-22 93.6
MS.gene65148.t1 MTR_7g086780 42.105 114 55 1 77 179 100 213 1.27e-22 98.6
MS.gene65148.t1 MTR_6g027450 66.667 54 18 0 77 130 90 143 5.69e-22 95.5
MS.gene65148.t1 MTR_4g082060 69.231 52 16 0 78 129 124 175 6.13e-22 96.7
MS.gene65148.t1 MTR_5g041400 58.667 75 25 1 78 152 121 189 2.14e-21 94.4
MS.gene65148.t1 MTR_5g041420 61.290 62 24 0 78 139 140 201 2.58e-21 93.6
MS.gene65148.t1 MTR_5g041380 66.102 59 20 0 78 136 129 187 9.52e-21 92.4
MS.gene65148.t1 MTR_6g027460 57.377 61 24 1 71 129 89 149 1.21e-20 92.0
MS.gene65148.t1 MTR_5g041530 69.231 52 16 0 78 129 142 193 1.75e-20 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65148.t1 AT3G55370 38.320 381 156 18 1 338 1 345 1.49e-52 177
MS.gene65148.t1 AT3G55370 38.736 364 147 17 1 338 1 314 1.71e-51 174
MS.gene65148.t1 AT3G55370 40.645 310 123 14 48 338 92 359 1.31e-46 162
MS.gene65148.t1 AT2G37590 40.217 276 108 9 1 240 1 255 2.74e-45 158
MS.gene65148.t1 AT5G02460 86.842 76 10 0 55 130 74 149 7.66e-44 156
MS.gene65148.t1 AT1G07640 73.418 79 21 0 58 136 3 81 9.79e-37 134
MS.gene65148.t1 AT1G07640 73.418 79 21 0 58 136 59 137 1.45e-36 135
MS.gene65148.t1 AT1G07640 73.418 79 21 0 58 136 67 145 1.89e-36 135
MS.gene65148.t1 AT2G28810 46.711 152 49 6 1 127 1 145 2.08e-34 129
MS.gene65148.t1 AT5G60200 69.880 83 22 1 73 152 52 134 4.12e-34 126
MS.gene65148.t1 AT5G60850 61.957 92 32 1 63 154 38 126 2.56e-32 123
MS.gene65148.t1 AT2G28810 74.648 71 17 1 58 127 58 128 3.36e-32 123
MS.gene65148.t1 AT3G52440 72.464 69 19 0 73 141 22 90 4.17e-31 118
MS.gene65148.t1 AT3G52440 72.464 69 19 0 73 141 41 109 6.53e-31 118
MS.gene65148.t1 AT3G45610 68.571 70 22 0 73 142 37 106 1.26e-30 117
MS.gene65148.t1 AT1G51700 67.500 80 24 2 69 147 27 105 5.00e-30 114
MS.gene65148.t1 AT5G65590 54.639 97 37 2 35 131 7 96 6.18e-30 117
MS.gene65148.t1 AT3G21270 48.905 137 57 3 77 213 30 153 2.00e-29 112
MS.gene65148.t1 AT1G28310 83.929 56 9 0 77 132 28 83 2.92e-29 115
MS.gene65148.t1 AT1G28310 83.929 56 9 0 77 132 52 107 4.62e-29 115
MS.gene65148.t1 AT1G21340 61.176 85 31 1 78 162 40 122 6.56e-29 112
MS.gene65148.t1 AT4G21050 39.881 168 73 4 74 213 22 189 2.50e-28 110
MS.gene65148.t1 AT5G62940 48.438 128 59 4 1 126 1 123 2.91e-28 113
MS.gene65148.t1 AT4G38000 62.651 83 29 1 70 152 35 115 7.21e-28 109
MS.gene65148.t1 AT5G66940 51.351 111 42 4 37 145 2 102 8.86e-28 108
MS.gene65148.t1 AT3G50410 57.471 87 34 2 50 133 2 88 1.08e-27 109
MS.gene65148.t1 AT3G61850 54.455 101 41 3 38 133 31 131 1.37e-27 110
MS.gene65148.t1 AT3G61850 54.455 101 41 3 38 133 19 119 1.37e-27 110
MS.gene65148.t1 AT3G61850 54.455 101 41 3 38 133 19 119 1.37e-27 110
MS.gene65148.t1 AT3G61850 54.455 101 41 3 38 133 19 119 1.94e-27 110
MS.gene65148.t1 AT3G61850 54.455 101 41 3 38 133 31 131 2.17e-27 110
MS.gene65148.t1 AT4G24060 72.131 61 17 0 70 130 47 107 2.44e-27 110
MS.gene65148.t1 AT2G28510 39.431 246 104 10 75 320 47 247 4.23e-26 105
MS.gene65148.t1 AT4G00940 74.576 59 14 1 72 130 65 122 5.68e-26 105
MS.gene65148.t1 AT4G00940 74.576 59 14 1 72 130 65 122 5.68e-26 105
MS.gene65148.t1 AT4G00940 74.576 59 14 1 72 130 65 122 5.68e-26 105
MS.gene65148.t1 AT1G64620 47.682 151 58 5 70 213 43 179 3.49e-24 102
MS.gene65148.t1 AT2G46590 77.358 53 10 1 73 123 63 115 9.37e-24 100
MS.gene65148.t1 AT2G46590 77.358 53 10 1 73 123 75 127 1.08e-23 100
MS.gene65148.t1 AT5G62430 73.077 52 14 0 78 129 56 107 1.61e-23 99.4
MS.gene65148.t1 AT3G47500 63.636 66 24 0 78 143 112 177 2.75e-23 100
MS.gene65148.t1 AT1G47655 78.723 47 10 0 78 124 31 77 1.51e-22 94.4
MS.gene65148.t1 AT1G26790 69.231 52 16 0 78 129 113 164 2.93e-22 97.1
MS.gene65148.t1 AT2G34140 71.154 52 15 0 78 129 60 111 3.18e-22 92.4
MS.gene65148.t1 AT1G29160 71.154 52 15 0 78 129 64 115 3.32e-22 92.4
MS.gene65148.t1 AT4G21040 72.727 55 14 1 78 132 27 80 5.18e-22 93.6
MS.gene65148.t1 AT5G39660 71.154 52 15 0 78 129 140 191 9.70e-22 96.3
MS.gene65148.t1 AT5G39660 71.154 52 15 0 78 129 140 191 9.70e-22 96.3
MS.gene65148.t1 AT5G39660 71.154 52 15 0 78 129 140 191 9.70e-22 96.3
MS.gene65148.t1 AT1G69570 69.231 52 16 0 78 129 134 185 2.62e-21 94.4
MS.gene65148.t1 AT4G21080 53.165 79 33 1 78 156 27 101 3.84e-21 91.7
MS.gene65148.t1 AT4G21030 42.667 75 43 0 74 148 19 93 1.14e-13 69.3

Find 110 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCTCTCCCACCATATA+TGG 0.222301 7.1:-42046604 MS.gene65148:CDS
CTCCAACCGGGACACTTCTA+AGG 0.229004 7.1:+42046103 None:intergenic
TTCCAATATTGTTCACTATT+AGG 0.263046 7.1:+42045508 None:intergenic
AAATAATTCTAGTGTGAAAA+TGG 0.280136 7.1:-42045599 MS.gene65148:CDS
ATCTCATCGGTGGTGGCGTT+GGG 0.300347 7.1:+42045965 None:intergenic
TTCCATATCGGAAGCTCTTT+AGG 0.302834 7.1:-42045847 MS.gene65148:CDS
GGTGATCACCTAAGTGAAAT+AGG 0.308942 7.1:-42045919 MS.gene65148:CDS
ATCCTAATAGTGAACAATAT+TGG 0.316935 7.1:-42045510 MS.gene65148:CDS
GAATCAATCTAGACAATTCT+TGG 0.322625 7.1:-42045572 MS.gene65148:CDS
GCAAGGCTTGTCGAAGGTAT+TGG 0.355195 7.1:-42046140 MS.gene65148:CDS
GTCTGAGCTTGCCGGCGACT+TGG 0.355952 7.1:+42046032 None:intergenic
AGTTGTTTGCAGAGCTAGTT+TGG 0.357904 7.1:+42046007 None:intergenic
CACCTAAAGAGCTTCCGATA+TGG 0.360566 7.1:+42045845 None:intergenic
GTTTGGCGGTCTGAGCTTGC+CGG 0.381911 7.1:+42046024 None:intergenic
CATGAGGTTGCGATGGTGTC+AGG 0.399816 7.1:+42046316 None:intergenic
ACCTGTTGATGCCAGTTGGC+TGG 0.401216 7.1:+42046579 None:intergenic
CAGTTGGCTGGATCCATATA+TGG 0.404780 7.1:+42046591 None:intergenic
AATCTCATCGGTGGTGGCGT+TGG 0.416328 7.1:+42045964 None:intergenic
GGAGACTTAAATTTCCATAT+CGG 0.426564 7.1:-42045859 MS.gene65148:CDS
CTTCGACAACCACCTCCAAC+CGG 0.428779 7.1:+42046090 None:intergenic
TGAGCTTGCCGGCGACTTGG+AGG 0.438606 7.1:+42046035 None:intergenic
TCCAGCCAACTGGCATCAAC+AGG 0.444071 7.1:-42046580 MS.gene65148:intron
CTACTACAAATTCCAACTAT+AGG 0.452160 7.1:-42045414 MS.gene65148:CDS
AAGGGAAATTGATGAGTTTG+TGG 0.457842 7.1:+42045742 None:intergenic
CCGCTACCACCACCTTCAAA+TGG 0.459662 7.1:+42045676 None:intergenic
AGTGAAATAGGACTAAACTA+TGG 0.460875 7.1:-42045907 MS.gene65148:CDS
GCCCTATCTGCCATCGAACC+TGG 0.462544 7.1:+42046279 None:intergenic
GCCACCACCGATGAGATTCA+TGG 0.464462 7.1:-42045959 MS.gene65148:CDS
AATCAATCTAGACAATTCTT+GGG 0.469852 7.1:-42045571 MS.gene65148:CDS
GACCAGGTTCGATGGCAGAT+AGG 0.475722 7.1:-42046281 MS.gene65148:CDS
TGCGTATGGTGGTGTGTCTA+AGG 0.477118 7.1:-42045629 MS.gene65148:CDS
CATATATGGATCCAGCCAAC+TGG 0.482920 7.1:-42046590 MS.gene65148:CDS
ATGGCTCCTTTGCATCATCT+AGG 0.483090 7.1:-42045940 MS.gene65148:CDS
ATAAAGTTATGTCCTATAGT+TGG 0.484745 7.1:+42045402 None:intergenic
ACCAGGTTCGATGGCAGATA+GGG 0.488883 7.1:-42046280 MS.gene65148:CDS
GAAGGTGGTGGTAGCGGTGG+TGG 0.489455 7.1:-42045670 MS.gene65148:CDS
AGGTTCGATGGTTGAGGCTG+TGG 0.490154 7.1:+42046336 None:intergenic
TCTACTCCAATGGGAGGTGT+TGG 0.490285 7.1:-42045880 MS.gene65148:CDS
TTCGACAAGCCTTGCAGAAG+TGG 0.490902 7.1:+42046148 None:intergenic
CATTGCTCAAAATTGCCAAC+AGG 0.493075 7.1:+42045772 None:intergenic
CCATTTGAAGGTGGTGGTAG+CGG 0.496097 7.1:-42045676 MS.gene65148:CDS
CTTAGAAGTGTCCCGGTTGG+AGG 0.496913 7.1:-42046102 MS.gene65148:CDS
AGAAGTGTCCCGGTTGGAGG+TGG 0.505449 7.1:-42046099 MS.gene65148:CDS
TGTCGAAGGTATTGGACAAA+AGG 0.506162 7.1:-42046132 MS.gene65148:CDS
TTAGGTGGAGGCGTTGGTAA+CGG 0.512562 7.1:-42045829 MS.gene65148:CDS
AGTTTAGTCCTATTTCACTT+AGG 0.513240 7.1:+42045911 None:intergenic
AACGGTGGTGTCAACGGTGG+TGG 0.514797 7.1:-42045811 MS.gene65148:CDS
GATGGTTGAGGCTGTGGTGG+TGG 0.518267 7.1:+42046342 None:intergenic
GGATTGTTGTATCCATTTGA+AGG 0.522126 7.1:-42045688 MS.gene65148:CDS
TGGAATGCTTCTGCTGGTGG+TGG 0.522977 7.1:-42045490 MS.gene65148:CDS
TGCGATGGTGTCAGGTTCGA+TGG 0.530120 7.1:+42046324 None:intergenic
AGCTTCTAAAGTTGACAAGA+AGG 0.532028 7.1:+42045723 None:intergenic
GTAGCAAAACTTTGTGTTGG+TGG 0.539364 7.1:+42046200 None:intergenic
GTAGTAGCAAAACTTTGTGT+TGG 0.542073 7.1:+42046197 None:intergenic
AGCTCTTTAGGTGGAGGCGT+TGG 0.544707 7.1:-42045835 MS.gene65148:CDS
CTGACACCATCGCAACCTCA+TGG 0.545229 7.1:-42046315 MS.gene65148:CDS
CTCGATCAGACCAGGTTCGA+TGG 0.546965 7.1:-42046289 MS.gene65148:CDS
GCCATGAATCTCATCGGTGG+TGG 0.547604 7.1:+42045958 None:intergenic
CATGGAGGCTCGATCAGACC+AGG 0.549550 7.1:-42046297 MS.gene65148:CDS
TTGTATCCATTTGAAGGTGG+TGG 0.552400 7.1:-42045682 MS.gene65148:CDS
GCAGCCACGCCACTTCTGCA+AGG 0.554356 7.1:-42046157 MS.gene65148:CDS
CAACAACACCTCCAAGTCGC+CGG 0.555102 7.1:-42046043 MS.gene65148:CDS
ATCGGAAGCTCTTTAGGTGG+AGG 0.559518 7.1:-42045841 MS.gene65148:CDS
ATTTACCTGTTGATGCCAGT+TGG 0.559637 7.1:+42046575 None:intergenic
GTTGGTAACGGTGGTGTCAA+CGG 0.562708 7.1:-42045817 MS.gene65148:CDS
CAATATTGGAATGCTTCTGC+TGG 0.566637 7.1:-42045496 MS.gene65148:CDS
GCCCTTAGAAGTGTCCCGGT+TGG 0.569239 7.1:-42046105 MS.gene65148:CDS
TTCGATGGTTGAGGCTGTGG+TGG 0.570012 7.1:+42046339 None:intergenic
GTTGGGGAACTGAGTACCGC+AGG 0.570283 7.1:+42045982 None:intergenic
CATATCGGAAGCTCTTTAGG+TGG 0.571600 7.1:-42045844 MS.gene65148:CDS
GATGGCAGATAGGGCAAGGA+TGG 0.575021 7.1:-42046271 MS.gene65148:CDS
TAGACACACCACCATACGCA+TGG 0.575174 7.1:+42045632 None:intergenic
TCTCATCGGTGGTGGCGTTG+GGG 0.576658 7.1:+42045966 None:intergenic
TGTTTGCAGAGCTAGTTTGG+CGG 0.578980 7.1:+42046010 None:intergenic
AAGTCTCCAACACCTCCCAT+TGG 0.579899 7.1:+42045874 None:intergenic
TGGTGCCCTTAGAAGTGTCC+CGG 0.580450 7.1:-42046109 MS.gene65148:CDS
TTAGAAGCTTCTTCTTCACA+TGG 0.581519 7.1:-42045709 MS.gene65148:CDS
TATTGGAATGCTTCTGCTGG+TGG 0.586587 7.1:-42045493 MS.gene65148:CDS
TTCGACAACCACCTCCAACC+GGG 0.589154 7.1:+42046091 None:intergenic
GCTTCTAAAGTTGACAAGAA+GGG 0.589264 7.1:+42045724 None:intergenic
CAAGCCTTGCAGAAGTGGCG+TGG 0.593138 7.1:+42046153 None:intergenic
GCAGCAACAAAATCATCAAG+TGG 0.596192 7.1:-42046397 MS.gene65148:intron
GTGGTTGTCGAAGAAACAAA+AGG 0.598438 7.1:-42046080 MS.gene65148:CDS
CGAAGGTATTGGACAAAAGG+TGG 0.602113 7.1:-42046129 MS.gene65148:CDS
TTTGAAGGTGGTGGTAGCGG+TGG 0.603652 7.1:-42045673 MS.gene65148:CDS
GAGCCTCCATGAGGTTGCGA+TGG 0.604696 7.1:+42046309 None:intergenic
GGTGTCAGGTTCGATGGTTG+AGG 0.608734 7.1:+42046330 None:intergenic
TTGGCTGGATCCATATATGG+TGG 0.609224 7.1:+42046594 None:intergenic
GTTCGATGGCAGATAGGGCA+AGG 0.614474 7.1:-42046275 MS.gene65148:CDS
AGGACTGTTGACACTAGTGT+TGG 0.616145 7.1:+42046374 None:intergenic
TCCAACCGGGACACTTCTAA+GGG 0.619326 7.1:+42046104 None:intergenic
TTGTTGTATCCATTTGAAGG+TGG 0.620036 7.1:-42045685 MS.gene65148:CDS
TGATCACCTAGATGATGCAA+AGG 0.622493 7.1:+42045934 None:intergenic
TGGCTGGATCCATATATGGT+GGG 0.628650 7.1:+42046595 None:intergenic
GGTGGAGGCGTTGGTAACGG+TGG 0.630519 7.1:-42045826 MS.gene65148:CDS
AAAGGAGCCATGAATCTCAT+CGG 0.638198 7.1:+42045952 None:intergenic
ACTTCTGCAAGGCTTGTCGA+AGG 0.638852 7.1:-42046146 MS.gene65148:CDS
TGTGGTGGTGGAATAAGTAG+AGG 0.640901 7.1:+42046354 None:intergenic
GCTGTAGAAAGATCTGTCCA+AGG 0.641214 7.1:+42045454 None:intergenic
GTGGTGCTACTAATTCACCT+TGG 0.642878 7.1:-42045471 MS.gene65148:CDS
AGACACACCACCATACGCAT+GGG 0.645170 7.1:+42045633 None:intergenic
AATGATCAATCCCATGCGTA+TGG 0.649437 7.1:-42045643 MS.gene65148:CDS
GATCAATCCCATGCGTATGG+TGG 0.672453 7.1:-42045640 MS.gene65148:CDS
CTCTGCAAACAACTCTCCTG+CGG 0.677430 7.1:-42045998 MS.gene65148:CDS
GTGTTGGTGGAATCACACCT+TGG 0.687306 7.1:+42046213 None:intergenic
GGTCTGATCGAGCCTCCATG+AGG 0.689453 7.1:+42046300 None:intergenic
GGAGCCATGAATCTCATCGG+TGG 0.692277 7.1:+42045955 None:intergenic
GGTAACGGTGGTGTCAACGG+TGG 0.699310 7.1:-42045814 MS.gene65148:CDS
TGGGAGAGAAGTATAAACCA+TGG 0.714065 7.1:+42046614 None:intergenic
ACACCATCGCAACCTCATGG+AGG 0.720835 7.1:-42046312 MS.gene65148:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAATAAAAATTAA+TGG + chr7.1:42045602-42045621 None:intergenic 10.0%
!! GATACATAAAATCAAATATT+TGG + chr7.1:42045575-42045594 None:intergenic 15.0%
!! AAATAATTCTAGTGTGAAAA+TGG - chr7.1:42046421-42046440 MS.gene65148:intron 20.0%
! AATCAATCTAGACAATTCTT+GGG - chr7.1:42046449-42046468 MS.gene65148:intron 25.0%
! ATCCTAATAGTGAACAATAT+TGG - chr7.1:42046510-42046529 MS.gene65148:intron 25.0%
! ATTTGGTATATTCAAGAGAA+GGG + chr7.1:42045558-42045577 None:intergenic 25.0%
! TATTTGGTATATTCAAGAGA+AGG + chr7.1:42045559-42045578 None:intergenic 25.0%
! TTCCAATATTGTTCACTATT+AGG + chr7.1:42046515-42046534 None:intergenic 25.0%
AGTGAAATAGGACTAAACTA+TGG - chr7.1:42046113-42046132 MS.gene65148:CDS 30.0%
AGTTTAGTCCTATTTCACTT+AGG + chr7.1:42046112-42046131 None:intergenic 30.0%
ATCAACAGGTAAATCAACAA+GGG - chr7.1:42045454-42045473 MS.gene65148:CDS 30.0%
CTACTACAAATTCCAACTAT+AGG - chr7.1:42046606-42046625 MS.gene65148:CDS 30.0%
GAATCAATCTAGACAATTCT+TGG - chr7.1:42046448-42046467 MS.gene65148:intron 30.0%
GGAGACTTAAATTTCCATAT+CGG - chr7.1:42046161-42046180 MS.gene65148:CDS 30.0%
! TATGGTTTTTCTACTCCAAT+GGG - chr7.1:42046131-42046150 MS.gene65148:CDS 30.0%
! TGATTTTGTTGCTGCAAAAA+AGG + chr7.1:42045618-42045637 None:intergenic 30.0%
AACCATCACAAAAATGTCCA+AGG - chr7.1:42045790-42045809 MS.gene65148:CDS 35.0%
AAGGGAAATTGATGAGTTTG+TGG + chr7.1:42046281-42046300 None:intergenic 35.0%
CATCAACAGGTAAATCAACA+AGG - chr7.1:42045453-42045472 MS.gene65148:CDS 35.0%
GGATTGTTGTATCCATTTGA+AGG - chr7.1:42046332-42046351 MS.gene65148:CDS 35.0%
GTAGTAGCAAAACTTTGTGT+TGG + chr7.1:42045826-42045845 None:intergenic 35.0%
TCAACAAGGGTTCTTCAATT+AGG - chr7.1:42045467-42045486 MS.gene65148:CDS 35.0%
TTAGAAGCTTCTTCTTCACA+TGG - chr7.1:42046311-42046330 MS.gene65148:CDS 35.0%
TTGTTGTATCCATTTGAAGG+TGG - chr7.1:42046335-42046354 MS.gene65148:CDS 35.0%
! CTATGGTTTTTCTACTCCAA+TGG - chr7.1:42046130-42046149 MS.gene65148:CDS 35.0%
! TCTACTTCGATTTTACCTGT+TGG - chr7.1:42046233-42046252 MS.gene65148:CDS 35.0%
! TTTTGTGATGGTTCTTGCAT+AGG + chr7.1:42045783-42045802 None:intergenic 35.0%
!! AGCTTCTAAAGTTGACAAGA+AGG + chr7.1:42046300-42046319 None:intergenic 35.0%
!! GCTTCTAAAGTTGACAAGAA+GGG + chr7.1:42046299-42046318 None:intergenic 35.0%
AAAGGAGCCATGAATCTCAT+CGG + chr7.1:42046071-42046090 None:intergenic 40.0%
AATGATCAATCCCATGCGTA+TGG - chr7.1:42046377-42046396 MS.gene65148:CDS 40.0%
AGTTGTTTGCAGAGCTAGTT+TGG + chr7.1:42046016-42046035 None:intergenic 40.0%
ATTTACCTGTTGATGCCAGT+TGG + chr7.1:42045448-42045467 None:intergenic 40.0%
CAATATTGGAATGCTTCTGC+TGG - chr7.1:42046524-42046543 MS.gene65148:intron 40.0%
CATTGCTCAAAATTGCCAAC+AGG + chr7.1:42046251-42046270 None:intergenic 40.0%
GCAGCAACAAAATCATCAAG+TGG - chr7.1:42045623-42045642 MS.gene65148:CDS 40.0%
GGTGATCACCTAAGTGAAAT+AGG - chr7.1:42046101-42046120 MS.gene65148:CDS 40.0%
GTAGCAAAACTTTGTGTTGG+TGG + chr7.1:42045823-42045842 None:intergenic 40.0%
GTGGTTGTCGAAGAAACAAA+AGG - chr7.1:42045940-42045959 MS.gene65148:CDS 40.0%
TACTTCTCTCCCACCATATA+TGG - chr7.1:42045416-42045435 MS.gene65148:CDS 40.0%
TGATCACCTAGATGATGCAA+AGG + chr7.1:42046089-42046108 None:intergenic 40.0%
TTCCATATCGGAAGCTCTTT+AGG - chr7.1:42046173-42046192 MS.gene65148:CDS 40.0%
TTGTATCCATTTGAAGGTGG+TGG - chr7.1:42046338-42046357 MS.gene65148:CDS 40.0%
! CACCTTGGACATTTTTGTGA+TGG + chr7.1:42045795-42045814 None:intergenic 40.0%
! CTGTTGGCAATTTTGAGCAA+TGG - chr7.1:42046249-42046268 MS.gene65148:CDS 40.0%
! TTGGCAATTTTGAGCAATGG+AGG - chr7.1:42046252-42046271 MS.gene65148:CDS 40.0%
!! TGTCGAAGGTATTGGACAAA+AGG - chr7.1:42045888-42045907 MS.gene65148:CDS 40.0%
AGGACTGTTGACACTAGTGT+TGG + chr7.1:42045649-42045668 None:intergenic 45.0%
ATGGCTCCTTTGCATCATCT+AGG - chr7.1:42046080-42046099 MS.gene65148:CDS 45.0%
CACCTAAAGAGCTTCCGATA+TGG + chr7.1:42046178-42046197 None:intergenic 45.0%
CAGTTGGCTGGATCCATATA+TGG + chr7.1:42045432-42045451 None:intergenic 45.0%
CATATATGGATCCAGCCAAC+TGG - chr7.1:42045430-42045449 MS.gene65148:CDS 45.0%
CATATCGGAAGCTCTTTAGG+TGG - chr7.1:42046176-42046195 MS.gene65148:CDS 45.0%
GCTGTAGAAAGATCTGTCCA+AGG + chr7.1:42046569-42046588 None:intergenic 45.0%
TATTGGAATGCTTCTGCTGG+TGG - chr7.1:42046527-42046546 MS.gene65148:intron 45.0%
TGGCTGGATCCATATATGGT+GGG + chr7.1:42045428-42045447 None:intergenic 45.0%
TTGGCTGGATCCATATATGG+TGG + chr7.1:42045429-42045448 None:intergenic 45.0%
! GGTTTTTCTACTCCAATGGG+AGG - chr7.1:42046134-42046153 MS.gene65148:CDS 45.0%
! TGTTTGCAGAGCTAGTTTGG+CGG + chr7.1:42046013-42046032 None:intergenic 45.0%
!! CGAAGGTATTGGACAAAAGG+TGG - chr7.1:42045891-42045910 MS.gene65148:CDS 45.0%
!! GTGGTGCTACTAATTCACCT+TGG - chr7.1:42046549-42046568 MS.gene65148:intron 45.0%
!! TGTGGTGGTGGAATAAGTAG+AGG + chr7.1:42045669-42045688 None:intergenic 45.0%
AAGTCTCCAACACCTCCCAT+TGG + chr7.1:42046149-42046168 None:intergenic 50.0%
ACCAGGTTCGATGGCAGATA+GGG - chr7.1:42045740-42045759 MS.gene65148:CDS 50.0%
ACTTCTGCAAGGCTTGTCGA+AGG - chr7.1:42045874-42045893 MS.gene65148:CDS 50.0%
AGACACACCACCATACGCAT+GGG + chr7.1:42046390-42046409 None:intergenic 50.0%
ATCGGAAGCTCTTTAGGTGG+AGG - chr7.1:42046179-42046198 MS.gene65148:CDS 50.0%
CTCTGCAAACAACTCTCCTG+CGG - chr7.1:42046022-42046041 MS.gene65148:CDS 50.0%
GATCAATCCCATGCGTATGG+TGG - chr7.1:42046380-42046399 MS.gene65148:CDS 50.0%
TAGACACACCACCATACGCA+TGG + chr7.1:42046391-42046410 None:intergenic 50.0%
TCCAACCGGGACACTTCTAA+GGG + chr7.1:42045919-42045938 None:intergenic 50.0%
TTCGACAAGCCTTGCAGAAG+TGG + chr7.1:42045875-42045894 None:intergenic 50.0%
! CCATTTGAAGGTGGTGGTAG+CGG - chr7.1:42046344-42046363 MS.gene65148:CDS 50.0%
! GCAAGGCTTGTCGAAGGTAT+TGG - chr7.1:42045880-42045899 MS.gene65148:CDS 50.0%
! GTGTTGGTGGAATCACACCT+TGG + chr7.1:42045810-42045829 None:intergenic 50.0%
! TCTACTCCAATGGGAGGTGT+TGG - chr7.1:42046140-42046159 MS.gene65148:CDS 50.0%
! TGCGTATGGTGGTGTGTCTA+AGG - chr7.1:42046391-42046410 MS.gene65148:CDS 50.0%
!! GTTGGTAACGGTGGTGTCAA+CGG - chr7.1:42046203-42046222 MS.gene65148:CDS 50.0%
!! TTAGGTGGAGGCGTTGGTAA+CGG - chr7.1:42046191-42046210 MS.gene65148:CDS 50.0%
ACACCATCGCAACCTCATGG+AGG - chr7.1:42045708-42045727 MS.gene65148:CDS 55.0%
ACCTGTTGATGCCAGTTGGC+TGG + chr7.1:42045444-42045463 None:intergenic 55.0%
AGGTTCGATGGTTGAGGCTG+TGG + chr7.1:42045687-42045706 None:intergenic 55.0%
CAACAACACCTCCAAGTCGC+CGG - chr7.1:42045977-42045996 MS.gene65148:CDS 55.0%
CATGAGGTTGCGATGGTGTC+AGG + chr7.1:42045707-42045726 None:intergenic 55.0%
CCGCTACCACCACCTTCAAA+TGG + chr7.1:42046347-42046366 None:intergenic 55.0%
CTCCAACCGGGACACTTCTA+AGG + chr7.1:42045920-42045939 None:intergenic 55.0%
CTCGATCAGACCAGGTTCGA+TGG - chr7.1:42045731-42045750 MS.gene65148:CDS 55.0%
CTGACACCATCGCAACCTCA+TGG - chr7.1:42045705-42045724 MS.gene65148:CDS 55.0%
CTTCGACAACCACCTCCAAC+CGG + chr7.1:42045933-42045952 None:intergenic 55.0%
GACCAGGTTCGATGGCAGAT+AGG - chr7.1:42045739-42045758 MS.gene65148:CDS 55.0%
GATGGCAGATAGGGCAAGGA+TGG - chr7.1:42045749-42045768 MS.gene65148:CDS 55.0%
GCCACCACCGATGAGATTCA+TGG - chr7.1:42046061-42046080 MS.gene65148:CDS 55.0%
GCCATGAATCTCATCGGTGG+TGG + chr7.1:42046065-42046084 None:intergenic 55.0%
GGAGCCATGAATCTCATCGG+TGG + chr7.1:42046068-42046087 None:intergenic 55.0%
GGTGTCAGGTTCGATGGTTG+AGG + chr7.1:42045693-42045712 None:intergenic 55.0%
GTTCGATGGCAGATAGGGCA+AGG - chr7.1:42045745-42045764 MS.gene65148:CDS 55.0%
TCCAGCCAACTGGCATCAAC+AGG - chr7.1:42045440-42045459 MS.gene65148:CDS 55.0%
TGGAATGCTTCTGCTGGTGG+TGG - chr7.1:42046530-42046549 MS.gene65148:intron 55.0%
TTCGACAACCACCTCCAACC+GGG + chr7.1:42045932-42045951 None:intergenic 55.0%
TTCGATGGTTGAGGCTGTGG+TGG + chr7.1:42045684-42045703 None:intergenic 55.0%
! AATCTCATCGGTGGTGGCGT+TGG + chr7.1:42046059-42046078 None:intergenic 55.0%
! AGCTCTTTAGGTGGAGGCGT+TGG - chr7.1:42046185-42046204 MS.gene65148:CDS 55.0%
! ATCTCATCGGTGGTGGCGTT+GGG + chr7.1:42046058-42046077 None:intergenic 55.0%
! TGCGATGGTGTCAGGTTCGA+TGG + chr7.1:42045699-42045718 None:intergenic 55.0%
!! CTTAGAAGTGTCCCGGTTGG+AGG - chr7.1:42045918-42045937 MS.gene65148:CDS 55.0%
!! TGGTGCCCTTAGAAGTGTCC+CGG - chr7.1:42045911-42045930 MS.gene65148:CDS 55.0%
!! TTTGAAGGTGGTGGTAGCGG+TGG - chr7.1:42046347-42046366 MS.gene65148:CDS 55.0%
CAAGCCTTGCAGAAGTGGCG+TGG + chr7.1:42045870-42045889 None:intergenic 60.0%
CATGGAGGCTCGATCAGACC+AGG - chr7.1:42045723-42045742 MS.gene65148:CDS 60.0%
GAGCCTCCATGAGGTTGCGA+TGG + chr7.1:42045714-42045733 None:intergenic 60.0%
GATGGTTGAGGCTGTGGTGG+TGG + chr7.1:42045681-42045700 None:intergenic 60.0%
GCCCTATCTGCCATCGAACC+TGG + chr7.1:42045744-42045763 None:intergenic 60.0%
GGTCTGATCGAGCCTCCATG+AGG + chr7.1:42045723-42045742 None:intergenic 60.0%
GTTTGGCGGTCTGAGCTTGC+CGG + chr7.1:42045999-42046018 None:intergenic 60.0%
! AACGGTGGTGTCAACGGTGG+TGG - chr7.1:42046209-42046228 MS.gene65148:CDS 60.0%
! GGTAACGGTGGTGTCAACGG+TGG - chr7.1:42046206-42046225 MS.gene65148:CDS 60.0%
! GTTGGGGAACTGAGTACCGC+AGG + chr7.1:42046041-42046060 None:intergenic 60.0%
! TCTCATCGGTGGTGGCGTTG+GGG + chr7.1:42046057-42046076 None:intergenic 60.0%
!! AGAAGTGTCCCGGTTGGAGG+TGG - chr7.1:42045921-42045940 MS.gene65148:CDS 60.0%
!! GCCCTTAGAAGTGTCCCGGT+TGG - chr7.1:42045915-42045934 MS.gene65148:CDS 60.0%
GCAGCCACGCCACTTCTGCA+AGG - chr7.1:42045863-42045882 MS.gene65148:CDS 65.0%
GTCTGAGCTTGCCGGCGACT+TGG + chr7.1:42045991-42046010 None:intergenic 65.0%
! TGAGCTTGCCGGCGACTTGG+AGG + chr7.1:42045988-42046007 None:intergenic 65.0%
!! GAAGGTGGTGGTAGCGGTGG+TGG - chr7.1:42046350-42046369 MS.gene65148:CDS 65.0%
!! GGTGGAGGCGTTGGTAACGG+TGG - chr7.1:42046194-42046213 MS.gene65148:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 42045408 42046634 42045408 ID=MS.gene65148
chr7.1 mRNA 42045408 42046634 42045408 ID=MS.gene65148.t1;Parent=MS.gene65148
chr7.1 exon 42046581 42046634 42046581 ID=MS.gene65148.t1.exon1;Parent=MS.gene65148.t1
chr7.1 CDS 42046581 42046634 42046581 ID=cds.MS.gene65148.t1;Parent=MS.gene65148.t1
chr7.1 exon 42045408 42046415 42045408 ID=MS.gene65148.t1.exon2;Parent=MS.gene65148.t1
chr7.1 CDS 42045408 42046415 42045408 ID=cds.MS.gene65148.t1;Parent=MS.gene65148.t1
Gene Sequence

>MS.gene65148

ATGGTTTATACTTCTCTCCCACCATATATGGATCCAGCCAACTGGCATCAACAGGTAAATCAACAAGGGTTCTTCAATTAGGTTTCAAAAAGAAAGAAAAAAAAATAAACTCAAAAGATAAATATTCGAGCTTTACTTTTGTTTATACCCTTCTCTTGAATATACCAAATATTTGATTTTATGTATCTTTACCATTAATTTTTATTTCCTTTTTTGCAGCAACAAAATCATCAAGTGGCCAACACTAGTGTCAACAGTCCTCTACTTATTCCACCACCACAGCCTCAACCATCGAACCTGACACCATCGCAACCTCATGGAGGCTCGATCAGACCAGGTTCGATGGCAGATAGGGCAAGGATGGCAAACATACCTATGCAAGAACCATCACAAAAATGTCCAAGGTGTGATTCCACCAACACAAAGTTTTGCTACTACAACAACTATAGTCTCTCGCAGCCACGCCACTTCTGCAAGGCTTGTCGAAGGTATTGGACAAAAGGTGGTGCCCTTAGAAGTGTCCCGGTTGGAGGTGGTTGTCGAAGAAACAAAAGGTCTAAATCAAGCAGCAACAACACCTCCAAGTCGCCGGCAAGCTCAGACCGCCAAACTAGCTCTGCAAACAACTCTCCTGCGGTACTCAGTTCCCCAACGCCACCACCGATGAGATTCATGGCTCCTTTGCATCATCTAGGTGATCACCTAAGTGAAATAGGACTAAACTATGGTTTTTCTACTCCAATGGGAGGTGTTGGAGACTTAAATTTCCATATCGGAAGCTCTTTAGGTGGAGGCGTTGGTAACGGTGGTGTCAACGGTGGTGGTTCTACTTCGATTTTACCTGTTGGCAATTTTGAGCAATGGAGGATGCCACAAACTCATCAATTTCCCTTCTTGTCAACTTTAGAAGCTTCTTCTTCACATGGATTGTTGTATCCATTTGAAGGTGGTGGTAGCGGTGGTGGTGCTAATGATCAATCCCATGCGTATGGTGGTGTGTCTAAGGTATTATCAAATAATTCTAGTGTGAAAATGGAAGAGAATCAATCTAGACAATTCTTGGGAATGATCAACAAAAACAACATCAATAATAATAATAATAATCCTAATAGTGAACAATATTGGAATGCTTCTGCTGGTGGTGGTGCTACTAATTCACCTTGGACAGATCTTTCTACAGCTTATAACACTTCTTCAGCTACTACAAATTCCAACTATAGGACATAA
Protein sequence

>MS.gene65148.t1

MVYTSLPPYMDPANWHQQQQNHQVANTSVNSPLLIPPPQPQPSNLTPSQPHGGSIRPGSMADRARMANIPMQEPSQKCPRCDSTNTKFCYYNNYSLSQPRHFCKACRRYWTKGGALRSVPVGGGCRRNKRSKSSSNNTSKSPASSDRQTSSANNSPAVLSSPTPPPMRFMAPLHHLGDHLSEIGLNYGFSTPMGGVGDLNFHIGSSLGGGVGNGGVNGGGSTSILPVGNFEQWRMPQTHQFPFLSTLEASSSHGLLYPFEGGGSGGGANDQSHAYGGVSKVLSNNSSVKMEENQSRQFLGMINKNNINNNNNNPNSEQYWNASAGGGATNSPWTDLSTAYNTSSATTNSNYRT