AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65278

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65278.t1 MTR_2g034280 90.970 598 32 3 1 597 1 577 0.0 1094
MS.gene65278.t1 MTR_2g034250 79.139 604 102 7 1 597 1 587 0.0 950
MS.gene65278.t1 MTR_2g034260 79.700 601 103 8 1 597 1 586 0.0 947
MS.gene65278.t1 MTR_7g109580 43.504 508 267 6 91 597 68 556 4.61e-134 403
MS.gene65278.t1 MTR_1g086970 44.399 491 241 9 116 597 13 480 3.63e-128 385
MS.gene65278.t1 MTR_3g065980 28.935 432 274 8 181 596 127 541 3.24e-58 204
MS.gene65278.t1 MTR_7g027190 30.198 404 249 11 209 595 286 673 5.92e-47 175
MS.gene65278.t1 MTR_8g442410 31.701 388 247 12 216 595 148 525 5.45e-43 162
MS.gene65278.t1 MTR_7g074650 31.720 372 230 11 227 595 442 792 4.04e-41 159
MS.gene65278.t1 MTR_4g076140 28.731 449 235 13 214 595 39 469 7.59e-41 155
MS.gene65278.t1 MTR_4g104020 28.148 405 266 12 205 595 127 520 6.26e-40 153
MS.gene65278.t1 MTR_7g062120 28.133 391 259 9 214 595 363 740 2.38e-37 148
MS.gene65278.t1 MTR_4g064150 27.735 393 262 8 213 594 351 732 7.63e-37 146
MS.gene65278.t1 MTR_4g076020 28.312 385 217 8 227 595 88 429 1.85e-36 142
MS.gene65278.t1 MTR_3g022830 27.400 427 241 8 224 596 26 437 1.83e-35 139
MS.gene65278.t1 MTR_4g064200 27.013 385 259 9 218 594 279 649 1.94e-35 142
MS.gene65278.t1 MTR_2g026250 25.714 385 268 8 218 595 223 596 3.82e-35 140
MS.gene65278.t1 MTR_5g094450 26.893 383 265 9 220 595 158 532 3.96e-35 139
MS.gene65278.t1 MTR_4g102790 27.225 382 254 11 224 594 293 661 5.08e-35 140
MS.gene65278.t1 MTR_1g069725 27.720 386 253 10 213 594 99 462 1.12e-34 137
MS.gene65278.t1 MTR_4g133660 24.740 384 271 8 218 594 170 542 1.52e-34 138
MS.gene65278.t1 MTR_4g064160 25.700 393 256 8 218 594 311 683 1.97e-33 136
MS.gene65278.t1 MTR_3g056110 26.425 386 259 9 220 595 172 542 2.12e-33 134
MS.gene65278.t1 MTR_2g097463 26.396 394 249 9 218 594 285 654 1.33e-32 133
MS.gene65278.t1 MTR_4g077760 26.440 382 259 9 220 595 190 555 1.94e-32 132
MS.gene65278.t1 MTR_2g097473 27.273 385 257 8 218 594 284 653 2.76e-32 132
MS.gene65278.t1 MTR_4g064120 27.649 387 254 12 212 585 243 616 3.65e-32 132
MS.gene65278.t1 MTR_2g097410 25.628 398 260 8 214 594 362 740 6.28e-32 132
MS.gene65278.t1 MTR_5g097480 25.519 337 236 6 220 550 174 501 3.40e-31 128
MS.gene65278.t1 MTR_2g097390 24.093 386 275 7 215 594 267 640 5.16e-31 128
MS.gene65278.t1 MTR_2g097467 26.234 385 261 8 218 594 284 653 6.06e-31 128
MS.gene65278.t1 MTR_2g097350 25.130 386 271 7 215 594 266 639 6.40e-31 128
MS.gene65278.t1 MTR_4g064180 26.494 385 261 10 212 585 243 616 1.37e-30 127
MS.gene65278.t1 MTR_2g082090 25.916 382 264 9 220 594 209 578 3.38e-30 125
MS.gene65278.t1 MTR_2g097310 24.093 386 275 7 215 594 264 637 3.39e-30 126
MS.gene65278.t1 MTR_3g072710 26.185 401 266 9 213 594 110 499 8.37e-30 123
MS.gene65278.t1 MTR_3g089055 25.840 387 252 11 224 595 204 570 2.84e-29 122
MS.gene65278.t1 MTR_3g089055 25.840 387 252 11 224 595 199 565 3.21e-29 122
MS.gene65278.t1 MTR_1g029420 25.065 387 255 9 224 595 226 592 1.86e-28 120
MS.gene65278.t1 MTR_1g096030 26.316 418 259 14 208 597 65 461 1.05e-26 114
MS.gene65278.t1 MTR_2g097380 24.586 362 226 10 239 594 240 560 1.84e-25 111
MS.gene65278.t1 MTR_4g097080 23.750 400 282 7 203 595 120 503 4.52e-25 109
MS.gene65278.t1 MTR_5g009080 31.907 257 157 7 189 444 25 264 6.02e-24 105
MS.gene65278.t1 MTR_5g009080 31.579 152 102 2 445 595 328 478 1.43e-19 92.4
MS.gene65278.t1 MTR_8g093070 26.952 397 251 14 210 594 126 495 7.37e-24 105
MS.gene65278.t1 MTR_5g015490 25.385 390 259 11 224 595 115 490 1.27e-22 102
MS.gene65278.t1 MTR_5g058860 23.173 479 318 15 148 594 36 496 2.46e-22 101
MS.gene65278.t1 MTR_4g026485 24.934 381 261 10 219 595 265 624 2.67e-21 98.6
MS.gene65278.t1 MTR_7g057230 25.679 405 267 13 205 594 271 656 2.54e-20 95.5
MS.gene65278.t1 MTR_2g099110 27.336 289 193 8 224 507 75 351 6.96e-20 93.2
MS.gene65278.t1 MTR_0092s0100 24.339 378 268 8 219 595 368 728 1.88e-18 89.7
MS.gene65278.t1 MTR_0092s0100 24.339 378 268 8 219 595 371 731 2.27e-18 89.7
MS.gene65278.t1 MTR_6g047750 29.242 277 163 11 337 595 401 662 4.75e-17 85.1
MS.gene65278.t1 MTR_8g020840 19.950 401 287 11 216 596 163 549 2.97e-13 72.8
MS.gene65278.t1 MTR_8g077940 25.627 359 220 14 222 562 181 510 1.17e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65278.t1 AT3G03450 31.139 395 246 8 210 596 170 546 1.06e-58 206
MS.gene65278.t1 AT1G66350 30.749 387 243 8 212 596 144 507 8.62e-56 197
MS.gene65278.t1 AT5G17490 31.008 387 243 9 214 596 151 517 4.08e-54 192
MS.gene65278.t1 AT2G01570 30.513 390 250 7 210 596 211 582 4.09e-54 194
MS.gene65278.t1 AT1G14920 31.282 390 247 8 210 596 159 530 9.18e-54 192
MS.gene65278.t1 AT5G41920 31.888 392 240 12 210 595 30 400 3.06e-45 165
MS.gene65278.t1 AT3G54220 31.622 370 234 8 227 594 297 649 3.15e-42 162
MS.gene65278.t1 AT1G07530 28.715 397 264 7 206 594 379 764 1.48e-40 158
MS.gene65278.t1 AT2G29060 28.278 389 260 7 215 594 276 654 1.26e-38 151
MS.gene65278.t1 AT2G29060 28.278 389 260 7 215 594 313 691 1.84e-38 151
MS.gene65278.t1 AT5G59450 29.716 387 253 11 218 595 222 598 2.05e-37 147
MS.gene65278.t1 AT3G46600 29.659 381 245 10 224 595 5 371 3.05e-37 142
MS.gene65278.t1 AT3G46600 29.306 389 252 10 216 595 205 579 5.71e-37 145
MS.gene65278.t1 AT1G55580 27.952 415 250 10 220 595 41 445 8.68e-37 143
MS.gene65278.t1 AT3G46600 29.306 389 252 10 216 595 173 547 1.28e-36 144
MS.gene65278.t1 AT2G29065 25.521 384 264 9 224 594 253 627 4.13e-35 140
MS.gene65278.t1 AT1G63100 25.000 400 265 10 215 594 269 653 8.26e-35 140
MS.gene65278.t1 AT1G63100 25.000 400 265 10 215 594 244 628 8.81e-35 139
MS.gene65278.t1 AT4G17230 25.907 386 261 9 220 595 155 525 7.92e-34 135
MS.gene65278.t1 AT4G17230 25.907 386 261 9 220 595 155 525 7.92e-34 135
MS.gene65278.t1 AT2G04890 25.464 377 259 7 224 595 54 413 7.95e-34 134
MS.gene65278.t1 AT5G66770 26.952 397 262 10 211 595 204 584 1.81e-33 135
MS.gene65278.t1 AT1G07520 24.937 397 279 9 209 594 140 528 2.71e-33 134
MS.gene65278.t1 AT1G07520 24.937 397 279 9 209 594 140 528 2.71e-33 134
MS.gene65278.t1 AT1G07520 24.937 397 279 9 209 594 304 692 3.36e-33 135
MS.gene65278.t1 AT1G07520 24.937 397 279 9 209 594 305 693 3.37e-33 135
MS.gene65278.t1 AT5G48150 24.219 384 271 9 220 595 119 490 5.38e-32 130
MS.gene65278.t1 AT5G48150 24.219 384 271 9 220 595 119 490 5.38e-32 130
MS.gene65278.t1 AT5G48150 24.219 384 271 9 220 595 119 490 5.38e-32 130
MS.gene65278.t1 AT5G48150 24.219 384 271 9 220 595 119 490 5.38e-32 130
MS.gene65278.t1 AT2G37650 26.357 387 269 6 214 595 326 701 2.98e-31 129
MS.gene65278.t1 AT2G37650 26.357 387 269 6 214 595 338 713 3.99e-31 129
MS.gene65278.t1 AT3G50650 26.462 359 231 10 251 595 203 542 2.25e-30 125
MS.gene65278.t1 AT1G50600 24.868 378 267 7 224 595 160 526 1.40e-29 123
MS.gene65278.t1 AT1G50600 24.868 378 267 7 224 595 231 597 2.89e-29 123
MS.gene65278.t1 AT1G50600 24.868 378 267 7 224 595 235 601 3.72e-29 122
MS.gene65278.t1 AT4G08250 25.547 411 264 12 204 594 88 476 1.52e-26 113
MS.gene65278.t1 AT1G21450 25.575 348 239 8 215 552 214 551 1.43e-22 102
MS.gene65278.t1 AT4G00150 26.923 390 235 14 218 595 203 554 5.83e-21 97.4
MS.gene65278.t1 AT1G50420 30.472 233 149 6 214 445 48 268 1.26e-20 95.9
MS.gene65278.t1 AT1G50420 31.410 156 95 4 446 595 330 479 2.03e-18 89.0
MS.gene65278.t1 AT2G45160 25.138 362 243 10 241 595 299 639 1.97e-20 95.9
MS.gene65278.t1 AT3G60630 25.914 301 202 8 306 595 332 622 1.96e-19 92.8
MS.gene65278.t1 AT4G37650 22.917 432 282 15 190 595 123 529 2.08e-18 89.4
MS.gene65278.t1 AT3G49950 24.103 390 264 12 224 594 31 407 6.05e-17 84.0
MS.gene65278.t1 AT5G52510 25.632 277 184 6 336 595 369 640 1.06e-15 80.9
MS.gene65278.t1 AT3G13840 25.623 281 185 10 221 490 145 412 3.51e-11 66.2

Find 74 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTTCTTTGCATCTTTA+AGG 0.061011 2.2:-54660799 None:intergenic
AATATCTCGACTGGTGTTAA+AGG 0.227086 2.2:-54660194 None:intergenic
CTTTGAAGTGTATGTGTTTC+CGG 0.229764 2.2:-54660933 None:intergenic
CTGTTTCTTTGTCGATTCTT+TGG 0.234871 2.2:-54660622 None:intergenic
TGTACTTCAGTTATGGTATT+AGG 0.241607 2.2:+54661341 MS.gene65278:CDS
TTCACTTGCTGAATCACTTT+TGG 0.248339 2.2:+54660469 MS.gene65278:CDS
TTGATTCTTAATCACCCTTT+TGG 0.256538 2.2:+54660404 MS.gene65278:CDS
GAGTCTCTTTGCTCTAATTT+TGG 0.258364 2.2:+54659972 MS.gene65278:CDS
CAAACAGAAACGGAGTTAAT+TGG 0.259899 2.2:-54661574 None:intergenic
TTATGGTATTAGGAATATTT+TGG 0.269222 2.2:+54661351 MS.gene65278:CDS
ATGGAAAACTTGTGGGAATT+TGG 0.283067 2.2:+54659816 MS.gene65278:CDS
AGATCCTTGCTAACCTCTTC+AGG 0.292320 2.2:-54660692 None:intergenic
ATAATTGTATTGAACGAAAA+AGG 0.305796 2.2:-54661013 None:intergenic
AAAGTTTCATCATCTTGATA+TGG 0.321809 2.2:-54660065 None:intergenic
TAATGAACCACACGCTTAAC+GGG 0.334947 2.2:-54660581 None:intergenic
CAATTAACTCCGTTTCTGTT+TGG 0.343724 2.2:+54661575 MS.gene65278:CDS
TCAATTGAAGCGCTTGCATT+AGG 0.365227 2.2:-54660865 None:intergenic
GAATTTGGTGAGTTCAGTTT+TGG 0.366424 2.2:+54659831 MS.gene65278:CDS
ATAATGAACCACACGCTTAA+CGG 0.369400 2.2:-54660582 None:intergenic
ACTAAATCAAAATCATTAAC+AGG 0.380198 2.2:-54660278 None:intergenic
AACATTGAAACTTTAACAAA+TGG 0.394609 2.2:-54660749 None:intergenic
AATGGTCATTGTCTTCTTGT+TGG 0.397166 2.2:+54661538 MS.gene65278:CDS
TCTTGCACATTTGATATGAA+TGG 0.412387 2.2:+54661520 MS.gene65278:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.418322 2.2:+54660623 MS.gene65278:CDS
GTCTTTGTATCAAGCTGAAT+TGG 0.419059 2.2:+54661471 MS.gene65278:CDS
AATGAGAGTAAGTTGTCAAC+TGG 0.424534 2.2:+54660314 MS.gene65278:CDS
TTGATCTCGAAATTCGTAAA+GGG 0.428901 2.2:+54660828 MS.gene65278:CDS
GATTCTTTGGCAAAGTGCTT+CGG 0.434208 2.2:-54660609 None:intergenic
GAAGATCTAGGAGACTCTAA+TGG 0.460843 2.2:+54659945 MS.gene65278:CDS
TGTCTTCTTGTTGGATGGAA+GGG 0.477569 2.2:+54661547 MS.gene65278:CDS
CATCATCTTGATATGGTTGG+TGG 0.478304 2.2:-54660058 None:intergenic
ATCTGTGATGGAAAACTTGT+GGG 0.486830 2.2:+54659809 None:intergenic
TCGGCTACGACCATTACTAT+AGG 0.488277 2.2:-54661184 None:intergenic
TTTCTCTTCATCAGAGAGAC+CGG 0.489642 2.2:-54660438 None:intergenic
AACTCTTTATCTTCGAAGAC+AGG 0.490618 2.2:+54660554 MS.gene65278:CDS
ATTGATCTCGAAATTCGTAA+AGG 0.493507 2.2:+54660827 MS.gene65278:CDS
GAATCACTGTACTTCAGTTA+TGG 0.497629 2.2:+54661334 MS.gene65278:CDS
GGAGATATCATGAAGATAGC+TGG 0.498257 2.2:+54660335 MS.gene65278:CDS
TTTCATCATCTTGATATGGT+TGG 0.499177 2.2:-54660061 None:intergenic
TTGTCTTCTTGTTGGATGGA+AGG 0.499303 2.2:+54661546 MS.gene65278:CDS
TTTCATTGTATCAAGTCCCT+TGG 0.502952 2.2:-54659901 None:intergenic
CCGGAGAAAGAAAATCCAAA+AGG 0.504198 2.2:-54660419 None:intergenic
GTCATTGTCTTCTTGTTGGA+TGG 0.509549 2.2:+54661542 MS.gene65278:CDS
CAAAATCATTAACAGGATGA+AGG 0.518378 2.2:-54660271 None:intergenic
CTCTCATGACTGTTTCAAGT+TGG 0.524579 2.2:-54661144 None:intergenic
AGAAGAAGCTCTTCTTGTGA+TGG 0.532512 2.2:-54660011 None:intergenic
ATCTTTATTTGATCCTGAAG+AGG 0.540063 2.2:+54660679 MS.gene65278:CDS
AAGGAATTTCCAAACAGAAA+CGG 0.541180 2.2:-54661584 None:intergenic
ACTTGAGCTTCTAAAGATAA+CGG 0.547553 2.2:+54660901 MS.gene65278:CDS
AACTATAAATCCTATAGTAA+TGG 0.549134 2.2:+54661174 MS.gene65278:CDS
CGGAGAAAGAAAATCCAAAA+GGG 0.549231 2.2:-54660418 None:intergenic
GTTATCTTTAGAAGCTCAAG+TGG 0.552221 2.2:-54660899 None:intergenic
AAATTCGTAAAGGGTTACAT+TGG 0.553017 2.2:+54660837 MS.gene65278:CDS
AAGATAACGGCTATTGCCAC+CGG 0.561951 2.2:+54660914 MS.gene65278:CDS
TCGACTGGTGTTAAAGGCGA+TGG 0.563201 2.2:-54660188 None:intergenic
TGTTTGGAAGATTGTATGAA+AGG 0.565315 2.2:+54661286 MS.gene65278:CDS
ATTGTGATTAGCTTCAGTCT+CGG 0.571904 2.2:-54661203 None:intergenic
TCTTCCTCAAAATCTATACC+AGG 0.583983 2.2:+54660380 MS.gene65278:CDS
AATCTGTGATGGAAAACTTG+TGG 0.585803 2.2:+54659808 None:intergenic
AGGTTATCACCTGATGATGA+AGG 0.586177 2.2:+54660242 MS.gene65278:CDS
TGATCCTGAAGAGGTTAGCA+AGG 0.599740 2.2:+54660688 MS.gene65278:CDS
CTGGTGTTAAAGGCGATGGT+TGG 0.604359 2.2:-54660184 None:intergenic
GTTGCTAAAAGATTTCCTTG+TGG 0.611038 2.2:+54661493 MS.gene65278:CDS
TGATAACTTCAACATTGACA+TGG 0.613825 2.2:+54660088 MS.gene65278:CDS
AGGTAACCCCGTTAAGCGTG+TGG 0.631363 2.2:+54660574 MS.gene65278:CDS
AAATTGAGAGTAAACTGCAA+CGG 0.631573 2.2:-54661092 None:intergenic
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.2:+54660342 MS.gene65278:CDS
CGAATGTGAAGATTGATGTG+TGG 0.657727 2.2:+54661398 MS.gene65278:CDS
GGGTGATTAAGAATCAAACC+TGG 0.658686 2.2:-54660398 None:intergenic
AAGCTTATAGTTGAAGACAC+CGG 0.666214 2.2:+54660953 MS.gene65278:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.2:+54660222 MS.gene65278:CDS
TGAAGTGTATGTGTTTCCGG+TGG 0.687997 2.2:-54660930 None:intergenic
AATGAACCACACGCTTAACG+GGG 0.713768 2.2:-54660580 None:intergenic
AATGTGCAAGAATTTCCACA+AGG 0.746520 2.2:-54661508 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTGAAACTTTAACAAA+TGG - chr2.2:54660752-54660771 None:intergenic 20.0%
!! AACTATAAATCCTATAGTAA+TGG + chr2.2:54661174-54661193 MS.gene65278:CDS 20.0%
!! ACTAAATCAAAATCATTAAC+AGG - chr2.2:54660281-54660300 None:intergenic 20.0%
!! ATAATTGTATTGAACGAAAA+AGG - chr2.2:54661016-54661035 None:intergenic 20.0%
!! ATATTGAAAAATTATGGCAA+AGG + chr2.2:54660212-54660231 MS.gene65278:CDS 20.0%
!!! TTATGGTATTAGGAATATTT+TGG + chr2.2:54661351-54661370 MS.gene65278:CDS 20.0%
! AAAGTTTCATCATCTTGATA+TGG - chr2.2:54660068-54660087 None:intergenic 25.0%
! GTCGAGATATTGAAAAATTA+TGG + chr2.2:54660206-54660225 MS.gene65278:CDS 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.2:54661426-54661445 MS.gene65278:CDS 25.0%
!!! TAATTTTTCAATATCTCGAC+TGG - chr2.2:54660206-54660225 None:intergenic 25.0%
!!! TTCATCCAATGATACTTTTT+GGG + chr2.2:54659875-54659894 MS.gene65278:CDS 25.0%
AAATTCGTAAAGGGTTACAT+TGG + chr2.2:54660837-54660856 MS.gene65278:CDS 30.0%
AAATTGAGAGTAAACTGCAA+CGG - chr2.2:54661095-54661114 None:intergenic 30.0%
AAGGAATTTCCAAACAGAAA+CGG - chr2.2:54661587-54661606 None:intergenic 30.0%
ATTGATCTCGAAATTCGTAA+AGG + chr2.2:54660827-54660846 MS.gene65278:CDS 30.0%
CAAAATCATTAACAGGATGA+AGG - chr2.2:54660274-54660293 None:intergenic 30.0%
CTCTTCGAAAAATGCAATAA+GGG - chr2.2:54660726-54660745 None:intergenic 30.0%
GAATCTTCTTTGCATCTTTA+AGG - chr2.2:54660802-54660821 None:intergenic 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.2:54661520-54661539 MS.gene65278:CDS 30.0%
TGATAACTTCAACATTGACA+TGG + chr2.2:54660088-54660107 MS.gene65278:CDS 30.0%
TGTACTTCAGTTATGGTATT+AGG + chr2.2:54661341-54661360 MS.gene65278:CDS 30.0%
TGTTTGGAAGATTGTATGAA+AGG + chr2.2:54661286-54661305 MS.gene65278:CDS 30.0%
TTGATCTCGAAATTCGTAAA+GGG + chr2.2:54660828-54660847 MS.gene65278:CDS 30.0%
TTTCATCATCTTGATATGGT+TGG - chr2.2:54660064-54660083 None:intergenic 30.0%
! ACTTGAGCTTCTAAAGATAA+CGG + chr2.2:54660901-54660920 MS.gene65278:CDS 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.2:54660679-54660698 MS.gene65278:CDS 30.0%
! GTTCATCCAATGATACTTTT+TGG + chr2.2:54659874-54659893 MS.gene65278:CDS 30.0%
! TCCATTTTGTGACACAAAAA+AGG + chr2.2:54660148-54660167 MS.gene65278:CDS 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.2:54660404-54660423 MS.gene65278:CDS 30.0%
! TTTTCTGTAGAAGAAGATCT+AGG + chr2.2:54659933-54659952 MS.gene65278:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.2:54661246-54661265 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.2:54661421-54661440 MS.gene65278:CDS 30.0%
!!! AAAATCCTCCAATTTTTTGC+CGG - chr2.2:54660975-54660994 None:intergenic 30.0%
!!! ATTAGGAATATTTTGGCAGA+AGG + chr2.2:54661358-54661377 MS.gene65278:CDS 30.0%
!!! TCCTTTTTTGTGTCACAAAA+TGG - chr2.2:54660152-54660171 None:intergenic 30.0%
AACTCTTTATCTTCGAAGAC+AGG + chr2.2:54660554-54660573 MS.gene65278:CDS 35.0%
AAGCTTATAGTTGAAGACAC+CGG + chr2.2:54660953-54660972 MS.gene65278:CDS 35.0%
AATGAGAGTAAGTTGTCAAC+TGG + chr2.2:54660314-54660333 MS.gene65278:CDS 35.0%
AATGGTCATTGTCTTCTTGT+TGG + chr2.2:54661538-54661557 MS.gene65278:CDS 35.0%
AATGTGCAAGAATTTCCACA+AGG - chr2.2:54661511-54661530 None:intergenic 35.0%
ATAATGAACCACACGCTTAA+CGG - chr2.2:54660585-54660604 None:intergenic 35.0%
ATGGAAAACTTGTGGGAATT+TGG + chr2.2:54659816-54659835 MS.gene65278:CDS 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.2:54660222-54660241 MS.gene65278:CDS 35.0%
CAAACAGAAACGGAGTTAAT+TGG - chr2.2:54661577-54661596 None:intergenic 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.2:54660623-54660642 MS.gene65278:CDS 35.0%
CAATTAACTCCGTTTCTGTT+TGG + chr2.2:54661575-54661594 MS.gene65278:CDS 35.0%
CGGAGAAAGAAAATCCAAAA+GGG - chr2.2:54660421-54660440 None:intergenic 35.0%
GAATCACTGTACTTCAGTTA+TGG + chr2.2:54661334-54661353 MS.gene65278:CDS 35.0%
GCTCTTCGAAAAATGCAATA+AGG - chr2.2:54660727-54660746 None:intergenic 35.0%
GTCTTTGTATCAAGCTGAAT+TGG + chr2.2:54661471-54661490 MS.gene65278:CDS 35.0%
GTTGCTAAAAGATTTCCTTG+TGG + chr2.2:54661493-54661512 MS.gene65278:CDS 35.0%
TCGAAAAATGCAATAAGGGT+TGG - chr2.2:54660722-54660741 None:intergenic 35.0%
TCTTCCTCAAAATCTATACC+AGG + chr2.2:54660380-54660399 MS.gene65278:CDS 35.0%
TTTCATTGTATCAAGTCCCT+TGG - chr2.2:54659904-54659923 None:intergenic 35.0%
! AATATCTCGACTGGTGTTAA+AGG - chr2.2:54660197-54660216 None:intergenic 35.0%
! AGGATTTTTCCTTCATCATC+AGG - chr2.2:54660254-54660273 None:intergenic 35.0%
! CAAACCTGGTATAGATTTTG+AGG - chr2.2:54660387-54660406 None:intergenic 35.0%
! CCTTTTGGATTTTCTTTCTC+CGG + chr2.2:54660419-54660438 MS.gene65278:CDS 35.0%
! GAGTCTCTTTGCTCTAATTT+TGG + chr2.2:54659972-54659991 MS.gene65278:CDS 35.0%
! GTGATACTTTTGTTGATCAG+TGG - chr2.2:54660039-54660058 None:intergenic 35.0%
! GTTATCTTTAGAAGCTCAAG+TGG - chr2.2:54660902-54660921 None:intergenic 35.0%
!! ATTGTGATTAGCTTCAGTCT+CGG - chr2.2:54661206-54661225 None:intergenic 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.2:54660625-54660644 None:intergenic 35.0%
!! CTTTGAAGTGTATGTGTTTC+CGG - chr2.2:54660936-54660955 None:intergenic 35.0%
!! GAATTTGGTGAGTTCAGTTT+TGG + chr2.2:54659831-54659850 MS.gene65278:CDS 35.0%
!! GGTATCCCAAAAAGTATCAT+TGG - chr2.2:54659883-54659902 None:intergenic 35.0%
!! TAGTGCGCTTTTTGATTGTT+TGG + chr2.2:54661270-54661289 MS.gene65278:CDS 35.0%
!! TCTTTTCGAGAAAGATTCTG+AGG + chr2.2:54661066-54661085 MS.gene65278:CDS 35.0%
!! TTCACTTGCTGAATCACTTT+TGG + chr2.2:54660469-54660488 MS.gene65278:CDS 35.0%
!!! GATACTTTTTGGGATACCAA+GGG + chr2.2:54659885-54659904 MS.gene65278:CDS 35.0%
!!! TGATACTTTTTGGGATACCA+AGG + chr2.2:54659884-54659903 MS.gene65278:CDS 35.0%
AGAAGAAGCTCTTCTTGTGA+TGG - chr2.2:54660014-54660033 None:intergenic 40.0%
CATCATCTTGATATGGTTGG+TGG - chr2.2:54660061-54660080 None:intergenic 40.0%
CCGGAGAAAGAAAATCCAAA+AGG - chr2.2:54660422-54660441 None:intergenic 40.0%
CGAATGTGAAGATTGATGTG+TGG + chr2.2:54661398-54661417 MS.gene65278:CDS 40.0%
GAAGATCTAGGAGACTCTAA+TGG + chr2.2:54659945-54659964 MS.gene65278:CDS 40.0%
GGAGATATCATGAAGATAGC+TGG + chr2.2:54660335-54660354 MS.gene65278:CDS 40.0%
GGGTGATTAAGAATCAAACC+TGG - chr2.2:54660401-54660420 None:intergenic 40.0%
GTCATTGTCTTCTTGTTGGA+TGG + chr2.2:54661542-54661561 MS.gene65278:CDS 40.0%
TAATGAACCACACGCTTAAC+GGG - chr2.2:54660584-54660603 None:intergenic 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.2:54660342-54660361 MS.gene65278:CDS 40.0%
TGAAGACACCGGCAAAAAAT+TGG + chr2.2:54660964-54660983 MS.gene65278:CDS 40.0%
TGTCTTCTTGTTGGATGGAA+GGG + chr2.2:54661547-54661566 MS.gene65278:CDS 40.0%
TTGTCTTCTTGTTGGATGGA+AGG + chr2.2:54661546-54661565 MS.gene65278:CDS 40.0%
TTTCTCTTCATCAGAGAGAC+CGG - chr2.2:54660441-54660460 None:intergenic 40.0%
! AGGTTATCACCTGATGATGA+AGG + chr2.2:54660242-54660261 MS.gene65278:CDS 40.0%
! CTCTCATGACTGTTTCAAGT+TGG - chr2.2:54661147-54661166 None:intergenic 40.0%
! GATTCTTTGGCAAAGTGCTT+CGG - chr2.2:54660612-54660631 None:intergenic 40.0%
! TCAATTGAAGCGCTTGCATT+AGG - chr2.2:54660868-54660887 None:intergenic 40.0%
!! GTTCAGTTTTGGTGTAACTG+AGG + chr2.2:54659842-54659861 MS.gene65278:CDS 40.0%
!! TTGGCTTGTGCTGAAAAAGT+TGG + chr2.2:54660488-54660507 MS.gene65278:CDS 40.0%
AATGAACCACACGCTTAACG+GGG - chr2.2:54660583-54660602 None:intergenic 45.0%
AGACACCGGCAAAAAATTGG+AGG + chr2.2:54660967-54660986 MS.gene65278:CDS 45.0%
AGATCCTTGCTAACCTCTTC+AGG - chr2.2:54660695-54660714 None:intergenic 45.0%
TCGGCTACGACCATTACTAT+AGG - chr2.2:54661187-54661206 None:intergenic 45.0%
TGAAGTGTATGTGTTTCCGG+TGG - chr2.2:54660933-54660952 None:intergenic 45.0%
TGATCCTGAAGAGGTTAGCA+AGG + chr2.2:54660688-54660707 MS.gene65278:CDS 45.0%
! AAGATAACGGCTATTGCCAC+CGG + chr2.2:54660914-54660933 MS.gene65278:CDS 45.0%
! CTGGTGTTAAAGGCGATGGT+TGG - chr2.2:54660187-54660206 None:intergenic 50.0%
! TCGACTGGTGTTAAAGGCGA+TGG - chr2.2:54660191-54660210 None:intergenic 50.0%
AGGTAACCCCGTTAAGCGTG+TGG + chr2.2:54660574-54660593 MS.gene65278:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 54659816 54661609 54659816 ID=MS.gene65278
chr2.2 mRNA 54659816 54661609 54659816 ID=MS.gene65278.t1;Parent=MS.gene65278
chr2.2 exon 54659816 54661609 54659816 ID=MS.gene65278.t1.exon1;Parent=MS.gene65278.t1
chr2.2 CDS 54659816 54661609 54659816 ID=cds.MS.gene65278.t1;Parent=MS.gene65278.t1
Gene Sequence

>MS.gene65278

ATGGAAAACTTGTGGGAATTTGGTGAGTTCAGTTTTGGTGTAACTGAGGATAAACTCAGTTCATCCAATGATACTTTTTGGGATACCAAGGGACTTGATACAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTCTAATGGAATTGAGTCTCTTTGCTCTAATTTTGGTCTCTTTCAAGATGATCCATCACAAGAAGAGCTTCTTCTTTCCACTGATCAACAAAAGTATCACCACCAACCATATCAAGATGATGAAACTTTTGATAACTTCAACATTGACATGGTTCACTTTGATGAACAACAATGTTCTACAAGAATTCTTCCATTTTGTGACACAAAAAAGGACAATCAATATTACCAACCATCGCCTTTAACACCAGTCGAGATATTGAAAAATTATGGCAAAGGATTCAAGAGGTTATCACCTGATGATGAAGGAAAAATCCTTCATCCTGTTAATGATTTTGATTTAGTTACAGATAATCAAAATGAGAGTAAGTTGTCAACTGGAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCCTCAAAATCTATACCAGGTTTGATTCTTAATCACCCTTTTGGATTTTCTTTCTCCGGTCTCTCTGATGAAGAGAAAGAAGATGTTTCACTTGCTGAATCACTTTTGGCTTGTGCTGAAAAAGTTGGATATCAACAATTTGAACGCGCTAGAAAATTGTTATCACATATCAACTCTTTATCTTCGAAGACAGGTAACCCCGTTAAGCGTGTGGTTCATTATTTTGCCGAAGCACTTTGCCAAAGAATCGACAAAGAAACAGGAAGAGTTTCATCTAACAATACACAAAAGATAGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAATCCAACCCTTATTGCATTTTTCGAAGAGCTTCCATTTGTTAAAGTTTCAATGTTCACATGTGTACAAGCTTTAATAGAAAACCTTAAAGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGTAAAGGGTTACATTGGACAATCCTAATGCAAGCGCTTCAATTGAGAACAGAATGTCCACTTGAGCTTCTAAAGATAACGGCTATTGCCACCGGAAACACATACACTTCAAAGCTTATAGTTGAAGACACCGGCAAAAAATTGGAGGATTTTGCACAAAGTTTGAACATACCTTTTTCGTTCAATACAATTATTGTATCTAACTTGTTACATCTAAGAGAAGATCTTTTCGAGAAAGATTCTGAGGAAACCGTTGCAGTTTACTCTCAATTTGCTCTTAGAAGCAACATTCAACAATCAGACCAACTTGAAACAGTCATGAGAGTTGTTAGAACTATAAATCCTATAGTAATGGTCGTAGCCGAGACTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCGCTTTTTGATTGTTTGGAAGATTGTATGAAAGGTGATGAGAAGAACAGAATGATTATAGAATCACTGTACTTCAGTTATGGTATTAGGAATATTTTGGCAGAAGGAATTGAAAGAAAGAGTACGAATGTGAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTTGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTTGCTAAAAGATTTCCTTGTGGAAATTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTGTTGGATGGAAGGGAACACCAATTAACTCCGTTTCTGTTTGGAAATTCCTTTGA
Protein sequence

>MS.gene65278.t1

MENLWEFGEFSFGVTEDKLSSSNDTFWDTKGLDTMKNVQFSVEEDLGDSNGIESLCSNFGLFQDDPSQEELLLSTDQQKYHHQPYQDDETFDNFNIDMVHFDEQQCSTRILPFCDTKKDNQYYQPSPLTPVEILKNYGKGFKRLSPDDEGKILHPVNDFDLVTDNQNESKLSTGDIMKIAGTRFIQSSSSSKSIPGLILNHPFGFSFSGLSDEEKEDVSLAESLLACAEKVGYQQFERARKLLSHINSLSSKTGNPVKRVVHYFAEALCQRIDKETGRVSSNNTQKIESLFDPEEVSKDLNPTLIAFFEELPFVKVSMFTCVQALIENLKDAKKIHVIDLEIRKGLHWTILMQALQLRTECPLELLKITAIATGNTYTSKLIVEDTGKKLEDFAQSLNIPFSFNTIIVSNLLHLREDLFEKDSEETVAVYSQFALRSNIQQSDQLETVMRVVRTINPIVMVVAETEANHNSKSFVNRFIEALFYFSALFDCLEDCMKGDEKNRMIIESLYFSYGIRNILAEGIERKSTNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGNSCTFDMNGHCLLVGWKGTPINSVSVWKFL