AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65313

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65313.t1 MTR_5g023910 93.534 232 13 2 1 230 1 232 3.34e-146 408
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65313.t1 AT4G35700 31.351 185 104 7 74 244 98 273 1.62e-17 80.1
MS.gene65313.t1 AT4G35610 54.000 50 23 0 105 154 129 178 2.34e-13 68.2

Find 97 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTTTCATCATCCTCTTC+TGG 0.204274 5.2:-14841629 None:intergenic
GTGGCTGGTGTGGTGTTGTT+TGG 0.232681 5.2:-14840223 None:intergenic
GCATCCAGAGCGTGCTCATC+GGG 0.273262 5.2:+14840597 MS.gene65313:CDS
GCAAACTCCCTCTTGCATTC+TGG 0.280646 5.2:-14840541 None:intergenic
CAGATGAACCGTCGGCAGTT+AGG 0.305066 5.2:-14840429 None:intergenic
TCTTGCATTCTGGACATGTT+GGG 0.316539 5.2:-14840531 None:intergenic
TGGCTGGTGTGGTGTTGTTT+GGG 0.349412 5.2:-14840222 None:intergenic
GCCACATGGAAAGCTGCGTT+TGG 0.354309 5.2:+14840562 MS.gene65313:CDS
TTCGCAGCCGGTGCAGGTGC+TGG 0.355655 5.2:-14841560 None:intergenic
CTCTTGCATTCTGGACATGT+TGG 0.358689 5.2:-14840532 None:intergenic
GTTGTTTGGGCTTGACTCAT+TGG 0.370557 5.2:-14840209 None:intergenic
GTGCAGGTGCAGGTGGTGCT+TGG 0.376757 5.2:-14841538 None:intergenic
GCCGGTGCAGGTGCTGGTGC+AGG 0.376765 5.2:-14841554 None:intergenic
AGATGAACCGTCGGCAGTTA+GGG 0.382592 5.2:-14840428 None:intergenic
TACCCTCATCACCACCATTT+GGG 0.382708 5.2:-14840369 None:intergenic
CTGATGGAGGGTCTTTCACT+TGG 0.398758 5.2:-14840504 None:intergenic
CCACATGGAAAGCTGCGTTT+GGG 0.399070 5.2:+14840563 MS.gene65313:CDS
GCAGGTGCTGGTGCAGGTGC+AGG 0.402832 5.2:-14841548 None:intergenic
TCTTCATTCGCAGCCGGTGC+AGG 0.409609 5.2:-14841566 None:intergenic
TTACCCTCATCACCACCATT+TGG 0.414014 5.2:-14840370 None:intergenic
TTTGGGCTTGACTCATTGGC+AGG 0.414848 5.2:-14840205 None:intergenic
GGGTCCTCTGCTGGTGGTAG+TGG 0.416926 5.2:+14841497 MS.gene65313:CDS
GACCATCAGAACCAACTTGT+TGG 0.436035 5.2:-14840456 None:intergenic
ATCATCATCATCAGTTAGTT+CGG 0.443434 5.2:-14840341 None:intergenic
TCCTCAGTGTTTGTCACAGC+GGG 0.444401 5.2:-14840283 None:intergenic
TTGTTTACAGGTGATGGTCT+TGG 0.452593 5.2:+14841395 MS.gene65313:intron
TCATCATCCTCTTCTGGAGG+TGG 0.459723 5.2:-14841623 None:intergenic
ATGGTCATGTCTTCAAGAAA+AGG 0.461097 5.2:+14840473 MS.gene65313:CDS
GTTCAGCCAAGGTGCGTTGA+GGG 0.469269 5.2:-14840249 None:intergenic
ATCCAACAAGTTGGTTCTGA+TGG 0.472826 5.2:+14840454 MS.gene65313:CDS
CAAGGTGCGTTGAGGGGAAG+TGG 0.482830 5.2:-14840242 None:intergenic
CGTCTTTCTTCATTCGCAGC+CGG 0.495573 5.2:-14841572 None:intergenic
GAATGAAGAAAGACGGAGAC+GGG 0.497570 5.2:+14841580 MS.gene65313:CDS
CACTTCCCCTCAACGCACCT+TGG 0.498843 5.2:+14840243 MS.gene65313:CDS
GAAAACACCGGAGGAAGTCC+TGG 0.499910 5.2:+14840318 MS.gene65313:CDS
CATGTTGGGGATCCTGATGG+AGG 0.503638 5.2:-14840517 None:intergenic
GGACATGTTGGGGATCCTGA+TGG 0.504468 5.2:-14840520 None:intergenic
CTTGCATTCTGGACATGTTG+GGG 0.506162 5.2:-14840530 None:intergenic
GGTGCTGGTGCAGGTGCAGG+TGG 0.509044 5.2:-14841545 None:intergenic
TCAACCGCAGGGTCCTCTGC+TGG 0.510398 5.2:+14841488 MS.gene65313:CDS
ATATATACCCTCAGCAGGAA+TGG 0.511286 5.2:-14840656 None:intergenic
CAAGTGAAAGACCCTCCATC+AGG 0.513855 5.2:+14840505 MS.gene65313:CDS
GTGCGTTGAGGGGAAGTGGC+TGG 0.516360 5.2:-14840238 None:intergenic
CTTGGCGACAAGATCAATGA+AGG 0.517419 5.2:+14841413 MS.gene65313:CDS
AGCATCCAGAGCGTGCTCAT+CGG 0.517955 5.2:+14840596 MS.gene65313:CDS
TATATACCCTCAGCAGGAAT+GGG 0.519334 5.2:-14840655 None:intergenic
GTCATATATATACCCTCAGC+AGG 0.521947 5.2:-14840661 None:intergenic
GATGATGATTTGATCCCAAA+TGG 0.535324 5.2:+14840355 MS.gene65313:CDS
TCATCTGTTATCCAACAAGT+TGG 0.537358 5.2:+14840445 MS.gene65313:CDS
GTTTCATCATCCTCTTCTGG+AGG 0.539642 5.2:-14841626 None:intergenic
CATCATCAGTTAGTTCGGCC+AGG 0.545384 5.2:-14840336 None:intergenic
ATGTTGGGGATCCTGATGGA+GGG 0.546248 5.2:-14840516 None:intergenic
AGTTCGGCCAGGACTTCCTC+CGG 0.548973 5.2:-14840325 None:intergenic
CGTTGCACCCATTCCTGCTG+AGG 0.553957 5.2:+14840648 MS.gene65313:CDS
ACCTGCACCAGCACCTGCAC+CGG 0.556575 5.2:+14841553 MS.gene65313:CDS
AGATCGATTCAATCAACCGC+AGG 0.558905 5.2:+14841476 MS.gene65313:CDS
TTGAGGGGAAGTGGCTGGTG+TGG 0.560312 5.2:-14840233 None:intergenic
ATGAGGGTAACGAAGACGAT+GGG 0.560313 5.2:+14840383 MS.gene65313:CDS
CGAGTTGCCACCTCCAGAAG+AGG 0.565350 5.2:+14841616 MS.gene65313:CDS
TCAGTGTTTGTCACAGCGGG+TGG 0.591498 5.2:-14840280 None:intergenic
GCAACGGTGCTAAAAGTTGG+TGG 0.591524 5.2:-14840631 None:intergenic
ACCGCAGGGTCCTCTGCTGG+TGG 0.592667 5.2:+14841491 MS.gene65313:CDS
CTCAGCAGGAATGGGTGCAA+CGG 0.592995 5.2:-14840647 None:intergenic
CATGTCTTCAAGAAAAGGAA+AGG 0.594271 5.2:+14840478 MS.gene65313:CDS
ATCCAGAGCGTGCTCATCGG+GGG 0.598304 5.2:+14840599 MS.gene65313:CDS
ACGGTGCTAAAAGTTGGTGG+AGG 0.599469 5.2:-14840628 None:intergenic
TTCAGCCAAGGTGCGTTGAG+GGG 0.602294 5.2:-14840248 None:intergenic
TTGGGCTTGACTCATTGGCA+GGG 0.602976 5.2:-14840204 None:intergenic
TGTTCAGCCAAGGTGCGTTG+AGG 0.606127 5.2:-14840250 None:intergenic
ATCCCAAATGGTGGTGATGA+GGG 0.607792 5.2:+14840367 MS.gene65313:CDS
CAACATGTCCAGAATGCAAG+AGG 0.611183 5.2:+14840533 MS.gene65313:CDS
TGGCTGATCATGTTCAGCCA+AGG 0.612094 5.2:-14840260 None:intergenic
TGGTAGTGGTCATATCACTA+AGG 0.615054 5.2:+14841511 MS.gene65313:CDS
TTGGCGACAAGATCAATGAA+GGG 0.623712 5.2:+14841414 MS.gene65313:CDS
GTTGCACCCATTCCTGCTGA+GGG 0.624536 5.2:+14840649 MS.gene65313:CDS
GATCCCAAATGGTGGTGATG+AGG 0.627502 5.2:+14840366 MS.gene65313:CDS
ATCCTCAGTGTTTGTCACAG+CGG 0.628423 5.2:-14840284 None:intergenic
CGGCTGCGAATGAAGAAAGA+CGG 0.631768 5.2:+14841573 MS.gene65313:CDS
GGTGCAACGGTGCTAAAAGT+TGG 0.632688 5.2:-14840634 None:intergenic
CGGTGCTAAAAGTTGGTGGA+GGG 0.635586 5.2:-14840627 None:intergenic
GCAAGAGGGAGTTTGCCACA+TGG 0.640414 5.2:+14840548 MS.gene65313:CDS
ATGACCACTACCACCAGCAG+AGG 0.645607 5.2:-14841501 None:intergenic
TTGGATAACAGATGAACCGT+CGG 0.650442 5.2:-14840437 None:intergenic
CGAATGAAGAAAGACGGAGA+CGG 0.650910 5.2:+14841579 MS.gene65313:CDS
GATCGATTCAATCAACCGCA+GGG 0.655948 5.2:+14841477 MS.gene65313:CDS
CATCCAGAGCGTGCTCATCG+GGG 0.656726 5.2:+14840598 MS.gene65313:CDS
TGGCGACAAGATCAATGAAG+GGG 0.669025 5.2:+14841415 MS.gene65313:CDS
GTTATCGCCCTAACTGCCGA+CGG 0.669691 5.2:+14840421 MS.gene65313:CDS
GATGAGGGTAACGAAGACGA+TGG 0.673127 5.2:+14840382 MS.gene65313:CDS
CCCAAACGCAGCTTTCCATG+TGG 0.686185 5.2:-14840563 None:intergenic
AACATGTCCAGAATGCAAGA+GGG 0.691308 5.2:+14840534 MS.gene65313:CDS
TGAGGGTAACGAAGACGATG+GGG 0.697277 5.2:+14840384 MS.gene65313:CDS
TGAAACCGTTCTGAAAACAC+CGG 0.698285 5.2:+14840306 MS.gene65313:CDS
AACCGTTCTGAAAACACCGG+AGG 0.709328 5.2:+14840309 MS.gene65313:CDS
ACCACCAGCAGAGGACCCTG+CGG 0.721168 5.2:-14841492 None:intergenic
GATGATTTGATCCCAAATGG+TGG 0.741325 5.2:+14840358 MS.gene65313:CDS
ACCCGCTGTGACAAACACTG+AGG 0.799006 5.2:+14840282 MS.gene65313:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGATTTGAAAAAAATTT+AGG + chr5.2:14841248-14841267 MS.gene65313:intron 10.0%
!! AAAAAATTAGATCTAAATCA+TGG - chr5.2:14840856-14840875 None:intergenic 15.0%
!! ATAAGACTATATATATAGTT+AGG - chr5.2:14840692-14840711 None:intergenic 15.0%
!! TAAGACTATATATATAGTTA+GGG - chr5.2:14840691-14840710 None:intergenic 15.0%
!!! TATTTTTGATGTTTTAGTTA+TGG + chr5.2:14840752-14840771 MS.gene65313:intron 15.0%
!! CTAAAACATTCAATTTGAAT+AGG - chr5.2:14841098-14841117 None:intergenic 20.0%
!!! AAAACATCTCAATTATCTTT+TGG - chr5.2:14840887-14840906 None:intergenic 20.0%
!!! AAATCATTTTGTTAAAAGAG+TGG - chr5.2:14841237-14841256 None:intergenic 20.0%
!!! GAGAAGTTTTATGAAAAGTA+TGG + chr5.2:14840826-14840845 MS.gene65313:intron 25.0%
ATAAAGCACACACTCATATA+AGG - chr5.2:14841360-14841379 None:intergenic 30.0%
ATCATCATCATCAGTTAGTT+CGG - chr5.2:14840344-14840363 None:intergenic 30.0%
TTACGTCGTTTATTGTTTAC+AGG + chr5.2:14841383-14841402 MS.gene65313:intron 30.0%
! ACATCTCAATTATCTTTTGG+TGG - chr5.2:14840884-14840903 None:intergenic 30.0%
ATGGTCATGTCTTCAAGAAA+AGG + chr5.2:14840473-14840492 MS.gene65313:CDS 35.0%
CATGTCTTCAAGAAAAGGAA+AGG + chr5.2:14840478-14840497 MS.gene65313:CDS 35.0%
CGTTTATTGTTTACAGGTGA+TGG + chr5.2:14841389-14841408 MS.gene65313:intron 35.0%
GATGATGATTTGATCCCAAA+TGG + chr5.2:14840355-14840374 MS.gene65313:CDS 35.0%
TCATCTGTTATCCAACAAGT+TGG + chr5.2:14840445-14840464 MS.gene65313:CDS 35.0%
AACATGTCCAGAATGCAAGA+GGG + chr5.2:14840534-14840553 MS.gene65313:CDS 40.0%
ATATATACCCTCAGCAGGAA+TGG - chr5.2:14840659-14840678 None:intergenic 40.0%
GATGATTTGATCCCAAATGG+TGG + chr5.2:14840358-14840377 MS.gene65313:CDS 40.0%
GTCATATATATACCCTCAGC+AGG - chr5.2:14840664-14840683 None:intergenic 40.0%
TATATACCCTCAGCAGGAAT+GGG - chr5.2:14840658-14840677 None:intergenic 40.0%
TCTGTTTCATCATCCTCTTC+TGG - chr5.2:14841632-14841651 None:intergenic 40.0%
TCTTGCATTCTGGACATGTT+GGG - chr5.2:14840534-14840553 None:intergenic 40.0%
TTGGATAACAGATGAACCGT+CGG - chr5.2:14840440-14840459 None:intergenic 40.0%
TTGGCGACAAGATCAATGAA+GGG + chr5.2:14841414-14841433 MS.gene65313:CDS 40.0%
! TTGTTTACAGGTGATGGTCT+TGG + chr5.2:14841395-14841414 MS.gene65313:intron 40.0%
!! ATCCAACAAGTTGGTTCTGA+TGG + chr5.2:14840454-14840473 MS.gene65313:CDS 40.0%
!! TGAAACCGTTCTGAAAACAC+CGG + chr5.2:14840306-14840325 MS.gene65313:CDS 40.0%
!! TGGTAGTGGTCATATCACTA+AGG + chr5.2:14841511-14841530 MS.gene65313:CDS 40.0%
ATCCTCAGTGTTTGTCACAG+CGG - chr5.2:14840287-14840306 None:intergenic 45.0%
ATGAGGGTAACGAAGACGAT+GGG + chr5.2:14840383-14840402 MS.gene65313:CDS 45.0%
CAACATGTCCAGAATGCAAG+AGG + chr5.2:14840533-14840552 MS.gene65313:CDS 45.0%
CGAATGAAGAAAGACGGAGA+CGG + chr5.2:14841579-14841598 MS.gene65313:CDS 45.0%
CTCTTGCATTCTGGACATGT+TGG - chr5.2:14840535-14840554 None:intergenic 45.0%
CTTGCATTCTGGACATGTTG+GGG - chr5.2:14840533-14840552 None:intergenic 45.0%
CTTGGCGACAAGATCAATGA+AGG + chr5.2:14841413-14841432 MS.gene65313:CDS 45.0%
GAATGAAGAAAGACGGAGAC+GGG + chr5.2:14841580-14841599 MS.gene65313:CDS 45.0%
GACCATCAGAACCAACTTGT+TGG - chr5.2:14840459-14840478 None:intergenic 45.0%
GTTGTTTGGGCTTGACTCAT+TGG - chr5.2:14840212-14840231 None:intergenic 45.0%
GTTTCATCATCCTCTTCTGG+AGG - chr5.2:14841629-14841648 None:intergenic 45.0%
TACCCTCATCACCACCATTT+GGG - chr5.2:14840372-14840391 None:intergenic 45.0%
TGGCGACAAGATCAATGAAG+GGG + chr5.2:14841415-14841434 MS.gene65313:CDS 45.0%
TTACCCTCATCACCACCATT+TGG - chr5.2:14840373-14840392 None:intergenic 45.0%
! AGATCGATTCAATCAACCGC+AGG + chr5.2:14841476-14841495 MS.gene65313:CDS 45.0%
! ATCCCAAATGGTGGTGATGA+GGG + chr5.2:14840367-14840386 MS.gene65313:CDS 45.0%
! GATCGATTCAATCAACCGCA+GGG + chr5.2:14841477-14841496 MS.gene65313:CDS 45.0%
! TTCCTCCGGTGTTTTCAGAA+CGG - chr5.2:14840314-14840333 None:intergenic 45.0%
!!! AAAAAAATCTAAAATTATTT+AGG + chr5.2:14840961-14840980 MS.gene65313:intron 5.0%
!!! AAAAAATCTAAAATTATTTA+GGG + chr5.2:14840962-14840981 MS.gene65313:intron 5.0%
AGATGAACCGTCGGCAGTTA+GGG - chr5.2:14840431-14840450 None:intergenic 50.0%
ATGTTGGGGATCCTGATGGA+GGG - chr5.2:14840519-14840538 None:intergenic 50.0%
CAAGTGAAAGACCCTCCATC+AGG + chr5.2:14840505-14840524 MS.gene65313:CDS 50.0%
CATCATCAGTTAGTTCGGCC+AGG - chr5.2:14840339-14840358 None:intergenic 50.0%
CCACATGGAAAGCTGCGTTT+GGG + chr5.2:14840563-14840582 MS.gene65313:CDS 50.0%
CGGCTGCGAATGAAGAAAGA+CGG + chr5.2:14841573-14841592 MS.gene65313:CDS 50.0%
CGTCTTTCTTCATTCGCAGC+CGG - chr5.2:14841575-14841594 None:intergenic 50.0%
CTGATGGAGGGTCTTTCACT+TGG - chr5.2:14840507-14840526 None:intergenic 50.0%
GATGAGGGTAACGAAGACGA+TGG + chr5.2:14840382-14840401 MS.gene65313:CDS 50.0%
GCAAACTCCCTCTTGCATTC+TGG - chr5.2:14840544-14840563 None:intergenic 50.0%
TCCTCAGTGTTTGTCACAGC+GGG - chr5.2:14840286-14840305 None:intergenic 50.0%
TGAGGGTAACGAAGACGATG+GGG + chr5.2:14840384-14840403 MS.gene65313:CDS 50.0%
TGGCTGATCATGTTCAGCCA+AGG - chr5.2:14840263-14840282 None:intergenic 50.0%
TTGGGCTTGACTCATTGGCA+GGG - chr5.2:14840207-14840226 None:intergenic 50.0%
TTTGGGCTTGACTCATTGGC+AGG - chr5.2:14840208-14840227 None:intergenic 50.0%
! ACGAAGACGATGGGGCTTTT+AGG + chr5.2:14840392-14840411 MS.gene65313:CDS 50.0%
! GATCCCAAATGGTGGTGATG+AGG + chr5.2:14840366-14840385 MS.gene65313:CDS 50.0%
! GCAACGGTGCTAAAAGTTGG+TGG - chr5.2:14840634-14840653 None:intergenic 50.0%
! GGTGCAACGGTGCTAAAAGT+TGG - chr5.2:14840637-14840656 None:intergenic 50.0%
! TCATCATCCTCTTCTGGAGG+TGG - chr5.2:14841626-14841645 None:intergenic 50.0%
!! AACCGTTCTGAAAACACCGG+AGG + chr5.2:14840309-14840328 MS.gene65313:CDS 50.0%
!! ACGGTGCTAAAAGTTGGTGG+AGG - chr5.2:14840631-14840650 None:intergenic 50.0%
!! CGGTGCTAAAAGTTGGTGGA+GGG - chr5.2:14840630-14840649 None:intergenic 50.0%
!! TGGCTGGTGTGGTGTTGTTT+GGG - chr5.2:14840225-14840244 None:intergenic 50.0%
ACCCGCTGTGACAAACACTG+AGG + chr5.2:14840282-14840301 MS.gene65313:CDS 55.0%
AGCATCCAGAGCGTGCTCAT+CGG + chr5.2:14840596-14840615 MS.gene65313:CDS 55.0%
ATGACCACTACCACCAGCAG+AGG - chr5.2:14841504-14841523 None:intergenic 55.0%
CAGATGAACCGTCGGCAGTT+AGG - chr5.2:14840432-14840451 None:intergenic 55.0%
CATGTTGGGGATCCTGATGG+AGG - chr5.2:14840520-14840539 None:intergenic 55.0%
CCCAAACGCAGCTTTCCATG+TGG - chr5.2:14840566-14840585 None:intergenic 55.0%
CTCAGCAGGAATGGGTGCAA+CGG - chr5.2:14840650-14840669 None:intergenic 55.0%
GAAAACACCGGAGGAAGTCC+TGG + chr5.2:14840318-14840337 MS.gene65313:CDS 55.0%
GCAAGAGGGAGTTTGCCACA+TGG + chr5.2:14840548-14840567 MS.gene65313:CDS 55.0%
GCCACATGGAAAGCTGCGTT+TGG + chr5.2:14840562-14840581 MS.gene65313:CDS 55.0%
GGACATGTTGGGGATCCTGA+TGG - chr5.2:14840523-14840542 None:intergenic 55.0%
GTTATCGCCCTAACTGCCGA+CGG + chr5.2:14840421-14840440 MS.gene65313:CDS 55.0%
GTTCAGCCAAGGTGCGTTGA+GGG - chr5.2:14840252-14840271 None:intergenic 55.0%
GTTGCACCCATTCCTGCTGA+GGG + chr5.2:14840649-14840668 MS.gene65313:CDS 55.0%
TCAGTGTTTGTCACAGCGGG+TGG - chr5.2:14840283-14840302 None:intergenic 55.0%
TGTTCAGCCAAGGTGCGTTG+AGG - chr5.2:14840253-14840272 None:intergenic 55.0%
TTCAGCCAAGGTGCGTTGAG+GGG - chr5.2:14840251-14840270 None:intergenic 55.0%
!! GTGGCTGGTGTGGTGTTGTT+TGG - chr5.2:14840226-14840245 None:intergenic 55.0%
!!! CGATGGGGCTTTTAGGACGA+TGG + chr5.2:14840399-14840418 MS.gene65313:CDS 55.0%
AGTTCGGCCAGGACTTCCTC+CGG - chr5.2:14840328-14840347 None:intergenic 60.0%
ATCCAGAGCGTGCTCATCGG+GGG + chr5.2:14840599-14840618 MS.gene65313:CDS 60.0%
CAAGGTGCGTTGAGGGGAAG+TGG - chr5.2:14840245-14840264 None:intergenic 60.0%
CACTTCCCCTCAACGCACCT+TGG + chr5.2:14840243-14840262 MS.gene65313:CDS 60.0%
CATCCAGAGCGTGCTCATCG+GGG + chr5.2:14840598-14840617 MS.gene65313:CDS 60.0%
CGAGTTGCCACCTCCAGAAG+AGG + chr5.2:14841616-14841635 MS.gene65313:CDS 60.0%
CGTTGCACCCATTCCTGCTG+AGG + chr5.2:14840648-14840667 MS.gene65313:CDS 60.0%
GCATCCAGAGCGTGCTCATC+GGG + chr5.2:14840597-14840616 MS.gene65313:CDS 60.0%
TCTTCATTCGCAGCCGGTGC+AGG - chr5.2:14841569-14841588 None:intergenic 60.0%
TTGAGGGGAAGTGGCTGGTG+TGG - chr5.2:14840236-14840255 None:intergenic 60.0%
AACCCCCGATGAGCACGCTC+TGG - chr5.2:14840604-14840623 None:intergenic 65.0%
ACCACCAGCAGAGGACCCTG+CGG - chr5.2:14841495-14841514 None:intergenic 65.0%
ACCTGCACCAGCACCTGCAC+CGG + chr5.2:14841553-14841572 MS.gene65313:CDS 65.0%
GGGTCCTCTGCTGGTGGTAG+TGG + chr5.2:14841497-14841516 MS.gene65313:CDS 65.0%
GTGCAGGTGCAGGTGGTGCT+TGG - chr5.2:14841541-14841560 None:intergenic 65.0%
GTGCGTTGAGGGGAAGTGGC+TGG - chr5.2:14840241-14840260 None:intergenic 65.0%
TCAACCGCAGGGTCCTCTGC+TGG + chr5.2:14841488-14841507 MS.gene65313:CDS 65.0%
ACCGCAGGGTCCTCTGCTGG+TGG + chr5.2:14841491-14841510 MS.gene65313:CDS 70.0%
TTCGCAGCCGGTGCAGGTGC+TGG - chr5.2:14841563-14841582 None:intergenic 70.0%
!! GCAGGTGCTGGTGCAGGTGC+AGG - chr5.2:14841551-14841570 None:intergenic 70.0%
!! GGTGCTGGTGCAGGTGCAGG+TGG - chr5.2:14841548-14841567 None:intergenic 70.0%
!! GCCGGTGCAGGTGCTGGTGC+AGG - chr5.2:14841557-14841576 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14840193 14841661 14840193 ID=MS.gene65313
chr5.2 mRNA 14840193 14841661 14840193 ID=MS.gene65313.t1;Parent=MS.gene65313
chr5.2 exon 14840193 14840670 14840193 ID=MS.gene65313.t1.exon1;Parent=MS.gene65313.t1
chr5.2 CDS 14840193 14840670 14840193 ID=cds.MS.gene65313.t1;Parent=MS.gene65313.t1
chr5.2 exon 14841405 14841661 14841405 ID=MS.gene65313.t1.exon2;Parent=MS.gene65313.t1
chr5.2 CDS 14841405 14841661 14841405 ID=cds.MS.gene65313.t1;Parent=MS.gene65313.t1
Gene Sequence

>MS.gene65313

ATGGAAAACGTCCCTGCCAATGAGTCAAGCCCAAACAACACCACACCAGCCACTTCCCCTCAACGCACCTTGGCTGAACATGATCAGCCACCCGCTGTGACAAACACTGAGGATGAAACCGTTCTGAAAACACCGGAGGAAGTCCTGGCCGAACTAACTGATGATGATGATTTGATCCCAAATGGTGGTGATGAGGGTAACGAAGACGATGGGGCTTTTAGGACGATGGTTATCGCCCTAACTGCCGACGGTTCATCTGTTATCCAACAAGTTGGTTCTGATGGTCATGTCTTCAAGAAAAGGAAAGGCAACCAAGTGAAAGACCCTCCATCAGGATCCCCAACATGTCCAGAATGCAAGAGGGAGTTTGCCACATGGAAAGCTGCGTTTGGGCATATGCGCAAGCATCCAGAGCGTGCTCATCGGGGGTTTTTCCCTCCACCAACTTTTAGCACCGTTGCACCCATTCCTGCTGAGGGTATATATATGACTAAACCCTAACTATATATATAGTCTTATTTTATAGATCTGAAAGTTTGTTTAAGATTTAAAAATAAATTATTTTTGATGTTTTAGTTATGGTTATGAGTGAAAGAATATTTAAAAAAAAATCATTTTGATATAAATAATTTAGAGAAGTTTTATGAAAAGTATGGTACTCCATGATTTAGATCTAATTTTTTTAGATCCACCAAAAGATAATTGAGATGTTTTAGTTGTTTAAAAAAAATATTTTTGATGTTTTAGCTATGTTTGTGTGTAATTTAAAAAAAAATCTAAAATTATTTAGGGATTATTTTTCAGAAACATTCAAGAAAAATATATTATGTTGCGTTTGTTTTAAATTGTAAAAAAGATTTTGTAAAAGTGATTTTCTTGAAAAGCTAATGAAAATAGCTTTTCCTATTCAAATTGAATGTTTTAGTGTTTAAGAAAAATATGTTATTTTGTGTTTGTCTAACTAAACTTAAAAAAAGAGATTTTATATTTCTCACTTATTTAAAAGCAAATAGAAATAGCTTTTCATTTTCAACATACAAACCACTCTTTTAACAAAATGATTTGAAAAAAATTTAGGCACACAAACTTAATTAAATCATAAAGAATAATTTTTTACTAATCTTAAATAAACAAATCAGACTGATTAACAAATCAACTTTTTAACCTTATATGAGTGTGTGCTTTATTTGTTACGTCGTTTATTGTTTACAGGTGATGGTCTTGGCGACAAGATCAATGAAGGGGTGAGCTCATTAGCTCGCGAGCTTGTGTTCGATCTGAATAGATCGATTCAATCAACCGCAGGGTCCTCTGCTGGTGGTAGTGGTCATATCACTAAGGAAATCCAAGCACCACCTGCACCTGCACCAGCACCTGCACCGGCTGCGAATGAAGAAAGACGGAGACGGGACTTTGATCTGAACGAGTTGCCACCTCCAGAAGAGGATGATGAAACAGAAGAGAAATGA
Protein sequence

>MS.gene65313.t1

MENVPANESSPNNTTPATSPQRTLAEHDQPPAVTNTEDETVLKTPEEVLAELTDDDDLIPNGGDEGNEDDGAFRTMVIALTADGSSVIQQVGSDGHVFKKRKGNQVKDPPSGSPTCPECKREFATWKAAFGHMRKHPERAHRGFFPPPTFSTVAPIPAEGDGLGDKINEGVSSLARELVFDLNRSIQSTAGSSAGGSGHITKEIQAPPAPAPAPAPAANEERRRRDFDLNELPPPEEDDETEEK