AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65320.t1 MTR_5g024070 94.551 312 16 1 1 312 20 330 0.0 600
MS.gene65320.t1 MTR_3g435550 37.037 135 77 2 162 295 38 165 1.91e-23 95.5
MS.gene65320.t1 MTR_3g091700 38.400 125 59 2 171 295 148 254 1.00e-20 89.7
MS.gene65320.t1 MTR_3g104830 48.276 87 40 2 180 264 393 476 1.85e-13 70.9
MS.gene65320.t1 MTR_3g115400 45.783 83 41 2 157 237 569 649 3.91e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65320.t1 AT4G35590 50.809 309 124 8 1 294 9 304 4.67e-87 264
MS.gene65320.t1 AT4G35590 50.809 309 124 8 1 294 59 354 2.43e-86 264
MS.gene65320.t1 AT1G74480 46.087 115 57 2 180 292 131 242 7.74e-24 99.4
MS.gene65320.t1 AT1G18790 44.628 121 61 3 175 293 120 236 8.37e-24 98.6
MS.gene65320.t1 AT5G66990 42.727 110 59 2 187 295 164 270 2.09e-21 92.0
MS.gene65320.t1 AT5G53040 42.609 115 53 1 181 295 147 248 3.64e-21 90.9
MS.gene65320.t1 AT5G16100 50.000 64 32 0 174 237 346 409 1.57e-13 71.2

Find 59 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATTACTTTATCAGATT+TGG 0.267461 5.2:-14941719 MS.gene65320:CDS
AAAAGGAAGTGCAGAGAATT+TGG 0.277802 5.2:-14941631 MS.gene65320:CDS
TGAAAGCTACTCCAATTCTT+AGG 0.296460 5.2:-14943647 MS.gene65320:CDS
AGAAGACGAGGCTGAGTTAC+TGG 0.328138 5.2:-14942063 MS.gene65320:CDS
CAGTCTCATTCATGATCTTC+AGG 0.329225 5.2:-14941569 MS.gene65320:intron
TTCTTTGACGCTTCTGCAAT+TGG 0.345585 5.2:+14941679 None:intergenic
TTTGGAATGGCCTGAAGCTT+TGG 0.353863 5.2:+14942254 None:intergenic
TTTCAGGAATCCAAAGCTTC+AGG 0.357774 5.2:-14942264 MS.gene65320:intron
GAGCTCTGCAAGGGAAGAAT+TGG 0.370187 5.2:-14942165 MS.gene65320:CDS
ATACCTTGTAACCAACAAAA+AGG 0.370413 5.2:+14943512 None:intergenic
GATTTCAACAACCTAAGAAT+TGG 0.374799 5.2:+14943636 None:intergenic
TGGAGATCATTAAGCAGGTT+TGG 0.377748 5.2:+14942236 None:intergenic
GTATTCCTCGCTGGCCTCAT+AGG 0.379146 5.2:-14941609 MS.gene65320:CDS
AAGGGTATAAGAATGGAGTT+TGG 0.381031 5.2:-14943590 MS.gene65320:CDS
TGATGCTAATTGCTAGGTTT+AGG 0.384292 5.2:+14940796 None:intergenic
AGTCTTGCCTGTGCTTAATC+AGG 0.387896 5.2:-14942108 MS.gene65320:CDS
TCTTCCCTTGCAGAGCTCTC+TGG 0.396713 5.2:+14942170 None:intergenic
CATAGAGAAGAGGCCCTTCA+TGG 0.404040 5.2:-14940917 MS.gene65320:CDS
TTTCTCCTCCATCACATTCA+CGG 0.441238 5.2:+14943721 None:intergenic
AAATTGAGACCAAGAGATTC+AGG 0.445679 5.2:-14940888 MS.gene65320:CDS
CGTCAAAGAATCTAAATGTT+GGG 0.449206 5.2:-14941666 MS.gene65320:CDS
AAAGATGTTGGAAAGTGAAA+AGG 0.459498 5.2:-14940944 MS.gene65320:CDS
TTGGGCTTACTGTTCTCAAA+AGG 0.466425 5.2:-14941648 MS.gene65320:CDS
TTGCCTTCTTATAACTGCCA+TGG 0.468291 5.2:+14940966 None:intergenic
TCAGCCTCGTCTTCTTCATA+AGG 0.476498 5.2:+14942071 None:intergenic
AACATCTTGCCTGAATCTCT+TGG 0.476779 5.2:+14940879 None:intergenic
CTCAATTTGTATGTCCATGA+AGG 0.479972 5.2:+14940903 None:intergenic
GAGAATTTGGTATTCCTCGC+TGG 0.487057 5.2:-14941618 MS.gene65320:CDS
GCAGGAAGAGGCAAAACATC+AGG 0.497528 5.2:-14941010 MS.gene65320:intron
TATAAGAAGGCAAAAGATGT+TGG 0.505266 5.2:-14940956 MS.gene65320:CDS
ATCATTAAGCAGGTTTGGAA+TGG 0.506660 5.2:+14942241 None:intergenic
AATTGGCATATCAAAGTACT+TGG 0.507062 5.2:+14941696 None:intergenic
TGAATGTGATGGAGGAGAAA+AGG 0.512445 5.2:-14943718 MS.gene65320:CDS
TCATGTGGAGATCATTAAGC+AGG 0.525296 5.2:+14942231 None:intergenic
AATCTTTGCAACATGGTCAC+TGG 0.527182 5.2:+14941735 None:intergenic
TTGGAATGTGAGAAAATTGA+GGG 0.529395 5.2:+14943535 None:intergenic
ATACAAGTTGGAACCGAAGA+AGG 0.529415 5.2:-14942207 MS.gene65320:CDS
ATCAGATGATGCTAATTGCT+AGG 0.533299 5.2:+14940790 None:intergenic
AGAGGCAAAACATCAGGAAA+GGG 0.535237 5.2:-14941004 MS.gene65320:CDS
GCGTCAAAGAATCTAAATGT+TGG 0.551834 5.2:-14941667 MS.gene65320:CDS
GTTGGAATGTGAGAAAATTG+AGG 0.552092 5.2:+14943534 None:intergenic
CAGCCATGGCAGTTATAAGA+AGG 0.552518 5.2:-14940969 MS.gene65320:CDS
TCTCCACATGATATACAAGT+TGG 0.555482 5.2:-14942219 MS.gene65320:CDS
AAGAGGCAAAACATCAGGAA+AGG 0.561722 5.2:-14941005 MS.gene65320:CDS
GTTCCAACTTGTATATCATG+TGG 0.569302 5.2:+14942216 None:intergenic
TTGAGATCCTGATTAAGCAC+AGG 0.580889 5.2:+14942101 None:intergenic
AAAAGGAAAACATAGAGAAG+AGG 0.595516 5.2:-14940927 MS.gene65320:CDS
GGAGGATAAAGATGCAGCCA+TGG 0.600209 5.2:-14940983 MS.gene65320:CDS
AGTGATTTGATCTTCCTATG+AGG 0.604696 5.2:+14941595 None:intergenic
TCAATTTGTATGTCCATGAA+GGG 0.616363 5.2:+14940904 None:intergenic
GGCAAAACATCAGGAAAGGG+AGG 0.624378 5.2:-14941001 MS.gene65320:CDS
TACACCAGAGAGCTCTGCAA+GGG 0.628005 5.2:-14942174 MS.gene65320:CDS
CATGTGTACCGTGAATGTGA+TGG 0.635852 5.2:-14943729 MS.gene65320:CDS
ATACACCAGAGAGCTCTGCA+AGG 0.645197 5.2:-14942175 MS.gene65320:CDS
TCTTCCTTATGAAGAAGACG+AGG 0.662936 5.2:-14942075 MS.gene65320:CDS
ATAAAGTAATCTTTGCAACA+TGG 0.664575 5.2:+14941728 None:intergenic
ATCTTCCTATGAGGCCAGCG+AGG 0.669880 5.2:+14941604 None:intergenic
TCTGCAAGGGAAGAATTGGA+AGG 0.677605 5.2:-14942161 MS.gene65320:CDS
GTGTACCGTGAATGTGATGG+AGG 0.689782 5.2:-14943726 MS.gene65320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAATAATAAATAAAAAAG+TGG + chr5.2:14943006-14943025 None:intergenic 10.0%
!!! TATACACTTATTTTATTAAT+AGG - chr5.2:14942630-14942649 MS.gene65320:intron 10.0%
!! AACAATTCAAATTCAATAAA+AGG + chr5.2:14941070-14941089 None:intergenic 15.0%
!! ACAATTCAAATTCAATAAAA+GGG + chr5.2:14941069-14941088 None:intergenic 15.0%
!! ATTATATGATTATGTATTCT+GGG - chr5.2:14941097-14941116 MS.gene65320:intron 15.0%
!!! TATTTTATTAATAGGAATCA+TGG - chr5.2:14942638-14942657 MS.gene65320:intron 15.0%
!! AAAGATTACTTTATCAGATT+TGG - chr5.2:14942823-14942842 MS.gene65320:intron 20.0%
!! AATACAAATTGATTAAAGCA+GGG + chr5.2:14941578-14941597 None:intergenic 20.0%
!! ACATTTATATATCATTCACA+GGG + chr5.2:14941652-14941671 None:intergenic 20.0%
!! ATACAGAAAAGATTTGTATA+AGG + chr5.2:14943242-14943261 None:intergenic 20.0%
!! ATGAAAATTTATAGTATCTG+AGG - chr5.2:14943199-14943218 MS.gene65320:intron 20.0%
!! GATTATATGATTATGTATTC+TGG - chr5.2:14941096-14941115 MS.gene65320:intron 20.0%
!! TAAAATAAGTGTATAAAGCT+AGG + chr5.2:14942625-14942644 None:intergenic 20.0%
!! TACATTTATATATCATTCAC+AGG + chr5.2:14941653-14941672 None:intergenic 20.0%
!! TATTTCAACCAAATACTATA+AGG + chr5.2:14943174-14943193 None:intergenic 20.0%
!! TTATAGACCAAATACTTTAT+AGG + chr5.2:14943424-14943443 None:intergenic 20.0%
!! TTGTAACTTAAACAAAATCA+CGG + chr5.2:14943325-14943344 None:intergenic 20.0%
!!! GAATATGTTTTTTGATTTCT+TGG - chr5.2:14942220-14942239 MS.gene65320:CDS 20.0%
!!! TATTGATTTCTTTATGTATG+TGG - chr5.2:14941837-14941856 MS.gene65320:intron 20.0%
!!! TTGAAAAACAGAATTTTCTA+CGG - chr5.2:14943714-14943733 MS.gene65320:CDS 20.0%
!!! TTTTTTAGGAAAGAAAAAGA+GGG - chr5.2:14942781-14942800 MS.gene65320:intron 20.0%
! AGTTATCTAATCTAACAAAC+AGG + chr5.2:14941795-14941814 None:intergenic 25.0%
! ATAAAGTAATCTTTGCAACA+TGG + chr5.2:14942817-14942836 None:intergenic 25.0%
! ATTGCAAACCTTATAGTATT+TGG - chr5.2:14943163-14943182 MS.gene65320:intron 25.0%
! CAATACAAATTGATTAAAGC+AGG + chr5.2:14941579-14941598 None:intergenic 25.0%
! CTAAATATTAGTTCAAGAGT+TGG + chr5.2:14943113-14943132 None:intergenic 25.0%
! GACATAATTAACTCAACATT+AGG + chr5.2:14942714-14942733 None:intergenic 25.0%
!! GTAAATCATAGAAGTTTTCT+TGG + chr5.2:14943088-14943107 None:intergenic 25.0%
!!! ATTCTGTTTTTCAATAACTC+TGG + chr5.2:14943708-14943727 None:intergenic 25.0%
!!! ATTTTTGAAGGGTATAAGAA+TGG - chr5.2:14940945-14940964 MS.gene65320:CDS 25.0%
!!! CATGTTCTTCTTTAATTTTC+AGG - chr5.2:14942262-14942281 MS.gene65320:intron 25.0%
!!! GTACCAATGTTTTTAAATAG+CGG - chr5.2:14941161-14941180 MS.gene65320:intron 25.0%
!!! GTTTTTTAGGAAAGAAAAAG+AGG - chr5.2:14942780-14942799 MS.gene65320:intron 25.0%
!!! TTTCTGAAGAGATTTTTGAA+GGG - chr5.2:14940934-14940953 MS.gene65320:CDS 25.0%
AAAAGGAAAACATAGAGAAG+AGG - chr5.2:14943615-14943634 MS.gene65320:CDS 30.0%
AAAGATGTTGGAAAGTGAAA+AGG - chr5.2:14943598-14943617 MS.gene65320:CDS 30.0%
ATACCTTGTAACCAACAAAA+AGG + chr5.2:14941033-14941052 None:intergenic 30.0%
ATTAAAGAAGAACATGACAG+AGG + chr5.2:14942258-14942277 None:intergenic 30.0%
ATTAGGAAGAAGTTATGCAT+TGG + chr5.2:14942697-14942716 None:intergenic 30.0%
CGTCAAAGAATCTAAATGTT+GGG - chr5.2:14942876-14942895 MS.gene65320:intron 30.0%
GATTTCAACAACCTAAGAAT+TGG + chr5.2:14940909-14940928 None:intergenic 30.0%
GGAATAAAATGTTCAAAAGC+AGG + chr5.2:14942591-14942610 None:intergenic 30.0%
GTTCAAAAATCAGTTACCAA+TGG + chr5.2:14941863-14941882 None:intergenic 30.0%
GTTGTCAATTAGCATATGTT+TGG - chr5.2:14943266-14943285 MS.gene65320:intron 30.0%
TAGCCGCTATTTAAAAACAT+TGG + chr5.2:14941167-14941186 None:intergenic 30.0%
TATAAGAAGGCAAAAGATGT+TGG - chr5.2:14943586-14943605 MS.gene65320:CDS 30.0%
TCAATTTGTATGTCCATGAA+GGG + chr5.2:14943641-14943660 None:intergenic 30.0%
TTATACAAAACAGTGTATCG+TGG + chr5.2:14941243-14941262 None:intergenic 30.0%
TTGGAATGTGAGAAAATTGA+GGG + chr5.2:14941010-14941029 None:intergenic 30.0%
TTGGTGAAGTTAGAATTTGA+GGG - chr5.2:14942089-14942108 MS.gene65320:CDS 30.0%
TTGTGAAATAGCAACTATAG+CGG - chr5.2:14941267-14941286 MS.gene65320:intron 30.0%
TTTGGTGAAGTTAGAATTTG+AGG - chr5.2:14942088-14942107 MS.gene65320:CDS 30.0%
! AAAACAGAATTTTCTACGGT+TGG - chr5.2:14943718-14943737 MS.gene65320:CDS 30.0%
! AATTGGCATATCAAAGTACT+TGG + chr5.2:14942849-14942868 None:intergenic 30.0%
! ACTGAAGCCTATAAAGTATT+TGG - chr5.2:14943414-14943433 MS.gene65320:intron 30.0%
! TTATGTATTCTGGGTTTGTT+TGG - chr5.2:14941106-14941125 MS.gene65320:intron 30.0%
! TTTTTAAATAGCGGCTATAG+CGG - chr5.2:14941170-14941189 MS.gene65320:intron 30.0%
!! ACAAATTTTGAAAGTTCAGC+AGG - chr5.2:14942510-14942529 MS.gene65320:intron 30.0%
!! GTTGTTTTCATAAGCTATCT+TGG + chr5.2:14941430-14941449 None:intergenic 30.0%
!! GTTTCTGAAGAGATTTTTGA+AGG - chr5.2:14940933-14940952 MS.gene65320:CDS 30.0%
AAATTGAGACCAAGAGATTC+AGG - chr5.2:14943654-14943673 MS.gene65320:CDS 35.0%
AAGTATGTTGTGGAACAGTT+AGG + chr5.2:14942069-14942088 None:intergenic 35.0%
ACTAAGTTGTTTATCCTGAC+AGG - chr5.2:14941523-14941542 MS.gene65320:intron 35.0%
AGGATGAAAATCAGACAACT+CGG + chr5.2:14941996-14942015 None:intergenic 35.0%
AGTATGTTGTGGAACAGTTA+GGG + chr5.2:14942068-14942087 None:intergenic 35.0%
AGTGATTTGATCTTCCTATG+AGG + chr5.2:14942950-14942969 None:intergenic 35.0%
ATCATTAAGCAGGTTTGGAA+TGG + chr5.2:14942304-14942323 None:intergenic 35.0%
CTAAGTTGTTTATCCTGACA+GGG - chr5.2:14941524-14941543 MS.gene65320:intron 35.0%
CTCAATTTGTATGTCCATGA+AGG + chr5.2:14943642-14943661 None:intergenic 35.0%
CTCGGATTTCAAATCGTATT+TGG + chr5.2:14941978-14941997 None:intergenic 35.0%
CTTGTAACCAACAAAAAGGT+TGG + chr5.2:14941029-14941048 None:intergenic 35.0%
GAAATGGTGCAGTAAATGTT+TGG - chr5.2:14941902-14941921 MS.gene65320:intron 35.0%
GAACAACGTGATAATTGACT+GGG + chr5.2:14942569-14942588 None:intergenic 35.0%
GAGATGTTGCAATGTGTATA+AGG + chr5.2:14943511-14943530 None:intergenic 35.0%
GAGTTCACTTGAAGATTGTT+AGG + chr5.2:14943489-14943508 None:intergenic 35.0%
GATTCCTTACAGTTACGAAA+TGG - chr5.2:14941886-14941905 MS.gene65320:intron 35.0%
GCGTCAAAGAATCTAAATGT+TGG - chr5.2:14942875-14942894 MS.gene65320:intron 35.0%
GTTCCAACTTGTATATCATG+TGG + chr5.2:14942329-14942348 None:intergenic 35.0%
GTTGGAATGTGAGAAAATTG+AGG + chr5.2:14941011-14941030 None:intergenic 35.0%
TAAAGTCCCTACACAAGTAA+CGG - chr5.2:14941700-14941719 MS.gene65320:CDS 35.0%
TCTCCACATGATATACAAGT+TGG - chr5.2:14942323-14942342 MS.gene65320:intron 35.0%
TGAAAGCTACTCCAATTCTT+AGG - chr5.2:14940895-14940914 MS.gene65320:CDS 35.0%
TTCTTTATGTATGTGGCCAT+TGG - chr5.2:14941844-14941863 MS.gene65320:intron 35.0%
TTGATAAAGTGAGTGTTTGC+AGG - chr5.2:14942758-14942777 MS.gene65320:intron 35.0%
TTTCGTAACTGTAAGGAATC+CGG + chr5.2:14941886-14941905 None:intergenic 35.0%
! AAAAGGAAGTGCAGAGAATT+TGG - chr5.2:14942911-14942930 MS.gene65320:intron 35.0%
! AAGGGTATAAGAATGGAGTT+TGG - chr5.2:14940952-14940971 MS.gene65320:CDS 35.0%
! ACTGTTCCACAACATACTTT+TGG - chr5.2:14942070-14942089 MS.gene65320:CDS 35.0%
! ACTTCACCAAAAGTATGTTG+TGG + chr5.2:14942079-14942098 None:intergenic 35.0%
! CATAGCGGAATTTGAACAAA+CGG - chr5.2:14941209-14941228 MS.gene65320:intron 35.0%
! GTATCTTTTCGTTCCTTCTT+CGG + chr5.2:14942351-14942370 None:intergenic 35.0%
! GTTATAGTGCTATTGCATAG+CGG - chr5.2:14941194-14941213 MS.gene65320:intron 35.0%
! TAGCTTTTGTGCCAAAATCA+CGG - chr5.2:14943349-14943368 MS.gene65320:intron 35.0%
! TATCTGAGGCTTCTTTAGAT+AGG - chr5.2:14943213-14943232 MS.gene65320:intron 35.0%
! TCACATTCCAACCTTTTTGT+TGG - chr5.2:14941019-14941038 MS.gene65320:intron 35.0%
!! CAACCTTTTTGTTGGTTACA+AGG - chr5.2:14941027-14941046 MS.gene65320:intron 35.0%
!! GAGTTTGTGTTTTCTGAGAA+TGG - chr5.2:14940858-14940877 MS.gene65320:CDS 35.0%
!! TGGTAACTGATTTTTGAACC+CGG - chr5.2:14941864-14941883 MS.gene65320:intron 35.0%
!!! ACAGGCAAGACTTTTTTTAG+TGG + chr5.2:14942426-14942445 None:intergenic 35.0%
!!! GCAAGACTTTTTTTAGTGGT+TGG + chr5.2:14942422-14942441 None:intergenic 35.0%
!!! TGAGTGTTTGCAGGTTTTTT+AGG - chr5.2:14942767-14942786 MS.gene65320:intron 35.0%
AACATCTTGCCTGAATCTCT+TGG + chr5.2:14943666-14943685 None:intergenic 40.0%
AAGAGGCAAAACATCAGGAA+AGG - chr5.2:14943537-14943556 MS.gene65320:CDS 40.0%
AATCTTTGCAACATGGTCAC+TGG + chr5.2:14942810-14942829 None:intergenic 40.0%
AGAACATGACAGAGGAAGTT+AGG + chr5.2:14942250-14942269 None:intergenic 40.0%
AGAGGCAAAACATCAGGAAA+GGG - chr5.2:14943538-14943557 MS.gene65320:CDS 40.0%
CAGTCTCATTCATGATCTTC+AGG - chr5.2:14942973-14942992 MS.gene65320:intron 40.0%
GAAGTTAGAATTTGAGGGCA+TGG - chr5.2:14942094-14942113 MS.gene65320:CDS 40.0%
GGAACAACGTGATAATTGAC+TGG + chr5.2:14942570-14942589 None:intergenic 40.0%
GGTGTGTACTGTGTACAAAT+TGG - chr5.2:14941625-14941644 MS.gene65320:CDS 40.0%
GTGCAGTAAATGTTTGGCAT+TGG - chr5.2:14941908-14941927 MS.gene65320:intron 40.0%
TAAAGCTAGGACTGAGTTGT+TGG + chr5.2:14942612-14942631 None:intergenic 40.0%
TATAGGCTTCAGTGCATGTT+TGG + chr5.2:14943407-14943426 None:intergenic 40.0%
TCATCCTACAGTTGCAATTG+CGG - chr5.2:14942009-14942028 MS.gene65320:intron 40.0%
TCATGTGGAGATCATTAAGC+AGG + chr5.2:14942314-14942333 None:intergenic 40.0%
TCTTCCTTATGAAGAAGACG+AGG - chr5.2:14942467-14942486 MS.gene65320:intron 40.0%
TGATTAAAGCAGGGAAACCT+TGG + chr5.2:14941569-14941588 None:intergenic 40.0%
TGCACCATTTCGTAACTGTA+AGG + chr5.2:14941893-14941912 None:intergenic 40.0%
TGGAGATCATTAAGCAGGTT+TGG + chr5.2:14942309-14942328 None:intergenic 40.0%
TTCGTAACTGTAAGGAATCC+GGG + chr5.2:14941885-14941904 None:intergenic 40.0%
TTCTTTGACGCTTCTGCAAT+TGG + chr5.2:14942866-14942885 None:intergenic 40.0%
TTGAGATCCTGATTAAGCAC+AGG + chr5.2:14942444-14942463 None:intergenic 40.0%
TTGCCTTCTTATAACTGCCA+TGG + chr5.2:14943579-14943598 None:intergenic 40.0%
TTGGGCTTACTGTTCTCAAA+AGG - chr5.2:14942894-14942913 MS.gene65320:intron 40.0%
TTGTGGAACAGTTAGGGAAA+TGG + chr5.2:14942062-14942081 None:intergenic 40.0%
TTTCAGGAATCCAAAGCTTC+AGG - chr5.2:14942278-14942297 MS.gene65320:intron 40.0%
TTTCTCCTCCATCACATTCA+CGG + chr5.2:14940824-14940843 None:intergenic 40.0%
! ATACAAGTTGGAACCGAAGA+AGG - chr5.2:14942335-14942354 MS.gene65320:intron 40.0%
! GGAGCATTTGAATCTAAACC+CGG + chr5.2:14942548-14942567 None:intergenic 40.0%
! TGAATGTGATGGAGGAGAAA+AGG - chr5.2:14940824-14940843 MS.gene65320:CDS 40.0%
!! GGCAAGATGTTTTCAAGAGA+AGG - chr5.2:14943675-14943694 MS.gene65320:CDS 40.0%
ACACATTGCAACATCTCTGC+AGG - chr5.2:14943514-14943533 MS.gene65320:intron 45.0%
AGCAGGTTTGAAAGTTCACC+GGG - chr5.2:14942527-14942546 MS.gene65320:intron 45.0%
AGTCTTGCCTGTGCTTAATC+AGG - chr5.2:14942434-14942453 MS.gene65320:intron 45.0%
CAGCAGGTTTGAAAGTTCAC+CGG - chr5.2:14942526-14942545 MS.gene65320:intron 45.0%
CAGCCATGGCAGTTATAAGA+AGG - chr5.2:14943573-14943592 MS.gene65320:CDS 45.0%
CATGTGTACCGTGAATGTGA+TGG - chr5.2:14940813-14940832 MS.gene65320:CDS 45.0%
GAGAATTTGGTATTCCTCGC+TGG - chr5.2:14942924-14942943 MS.gene65320:intron 45.0%
GATTCGCCGTTACTTGTGTA+GGG + chr5.2:14941709-14941728 None:intergenic 45.0%
GCTTCAGTGCATGTTTGGAT+TGG + chr5.2:14943402-14943421 None:intergenic 45.0%
TCAGCCTCGTCTTCTTCATA+AGG + chr5.2:14942474-14942493 None:intergenic 45.0%
TCTGCAAGGGAAGAATTGGA+AGG - chr5.2:14942381-14942400 MS.gene65320:intron 45.0%
TGATTCGCCGTTACTTGTGT+AGG + chr5.2:14941710-14941729 None:intergenic 45.0%
! CTTTTGTGCCAAAATCACGG+TGG - chr5.2:14943352-14943371 MS.gene65320:intron 45.0%
! TGCTGCAAATGTTGACTGCA+GGG - chr5.2:14942033-14942052 MS.gene65320:CDS 45.0%
! TTTGGAATGGCCTGAAGCTT+TGG + chr5.2:14942291-14942310 None:intergenic 45.0%
AAAGCAGGGAAACCTTGGTC+AGG + chr5.2:14941564-14941583 None:intergenic 50.0%
AGAAGACGAGGCTGAGTTAC+TGG - chr5.2:14942479-14942498 MS.gene65320:intron 50.0%
ATACACCAGAGAGCTCTGCA+AGG - chr5.2:14942367-14942386 MS.gene65320:intron 50.0%
CAGCACACAGAGCAGCAAAA+TGG - chr5.2:14941604-14941623 MS.gene65320:CDS 50.0%
CATAGAGAAGAGGCCCTTCA+TGG - chr5.2:14943625-14943644 MS.gene65320:CDS 50.0%
GAGCTCTGCAAGGGAAGAAT+TGG - chr5.2:14942377-14942396 MS.gene65320:intron 50.0%
GCAGGAAGAGGCAAAACATC+AGG - chr5.2:14943532-14943551 MS.gene65320:CDS 50.0%
GGAGGATAAAGATGCAGCCA+TGG - chr5.2:14943559-14943578 MS.gene65320:CDS 50.0%
GGCAAAACATCAGGAAAGGG+AGG - chr5.2:14943541-14943560 MS.gene65320:CDS 50.0%
GTGTACCGTGAATGTGATGG+AGG - chr5.2:14940816-14940835 MS.gene65320:CDS 50.0%
TACACCAGAGAGCTCTGCAA+GGG - chr5.2:14942368-14942387 MS.gene65320:intron 50.0%
TAGCAACTATAGCGGCGCTA+TGG - chr5.2:14941275-14941294 MS.gene65320:intron 50.0%
TGCAACATCTCTGCAGGAAG+AGG - chr5.2:14943520-14943539 MS.gene65320:CDS 50.0%
TGTTTATCCTGACAGGGCCT+TGG - chr5.2:14941530-14941549 MS.gene65320:intron 50.0%
TTGGTCAGGCCTAAAAGCCA+AGG + chr5.2:14941550-14941569 None:intergenic 50.0%
! CTGCTGCAAATGTTGACTGC+AGG - chr5.2:14942032-14942051 MS.gene65320:intron 50.0%
! TGGCATTGAGACCCGAATCA+CGG + chr5.2:14943382-14943401 None:intergenic 50.0%
! TTGAGGGCATGGTTCAGCAA+TGG - chr5.2:14942105-14942124 MS.gene65320:CDS 50.0%
!! TTGGCTTTTAGGCCTGACCA+AGG - chr5.2:14941549-14941568 MS.gene65320:intron 50.0%
ATCTTCCTATGAGGCCAGCG+AGG + chr5.2:14942941-14942960 None:intergenic 55.0%
CTAAAAGCCAAGGCCCTGTC+AGG + chr5.2:14941540-14941559 None:intergenic 55.0%
GCAGCCGCAATTGCAACTGT+AGG + chr5.2:14942016-14942035 None:intergenic 55.0%
GCTATGGCAGTGTTGCATGG+CGG - chr5.2:14941291-14941310 MS.gene65320:intron 55.0%
GGGCATGGTTCAGCAATGGA+TGG - chr5.2:14942109-14942128 MS.gene65320:CDS 55.0%
TCTTCCCTTGCAGAGCTCTC+TGG + chr5.2:14942375-14942394 None:intergenic 55.0%
! ACGGTGAGCCACCGTGATTT+TGG + chr5.2:14943363-14943382 None:intergenic 55.0%
! GTATTCCTCGCTGGCCTCAT+AGG - chr5.2:14942933-14942952 MS.gene65320:intron 55.0%
!! CTGACAGGGCCTTGGCTTTT+AGG - chr5.2:14941538-14941557 MS.gene65320:intron 55.0%
ACGGTGGCTCACCGTGATTC+GGG - chr5.2:14943368-14943387 MS.gene65320:intron 60.0%
CACGGTGGCTCACCGTGATT+CGG - chr5.2:14943367-14943386 MS.gene65320:intron 60.0%
GGCGCTATGGCAGTGTTGCA+TGG - chr5.2:14941288-14941307 MS.gene65320:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14940801 14943763 14940801 ID=MS.gene65320
chr5.2 mRNA 14940801 14943763 14940801 ID=MS.gene65320.t1;Parent=MS.gene65320
chr5.2 exon 14943516 14943763 14943516 ID=MS.gene65320.t1.exon1;Parent=MS.gene65320.t1
chr5.2 CDS 14943516 14943763 14943516 ID=cds.MS.gene65320.t1;Parent=MS.gene65320.t1
chr5.2 exon 14942033 14942280 14942033 ID=MS.gene65320.t1.exon2;Parent=MS.gene65320.t1
chr5.2 CDS 14942033 14942280 14942033 ID=cds.MS.gene65320.t1;Parent=MS.gene65320.t1
chr5.2 exon 14941570 14941784 14941570 ID=MS.gene65320.t1.exon3;Parent=MS.gene65320.t1
chr5.2 CDS 14941570 14941784 14941570 ID=cds.MS.gene65320.t1;Parent=MS.gene65320.t1
chr5.2 exon 14940801 14941028 14940801 ID=MS.gene65320.t1.exon4;Parent=MS.gene65320.t1
chr5.2 CDS 14940801 14941028 14940801 ID=cds.MS.gene65320.t1;Parent=MS.gene65320.t1
Gene Sequence

>MS.gene65320

ATGAGAAGCGTGCATGTGTACCGTGAATGTGATGGAGGAGAAAAGGAAGTTGAAAGAGAGTTTGTGTTTTCTGAGAATGGTTCTTATGTTGAAATGAAAGCTACTCCAATTCTTAGGTTGTTGAAATCTTTTGTTTCTGAAGAGATTTTTGAAGGGTATAAGAATGGAGTTTGGCTTTGTGTTTTTGCTTTTCATGCTTGTAATGTCCCTCAATTTTCTCACATTCCAACCTTTTTGTTGGTTACAAGGTATGTATATCTACTTACCCTTTTATTGAATTTGAATTGTTTCATTTGATTATATGATTATGTATTCTGGGTTTGTTTGGATTCACTTATTTGAACTTATAACTTATGACATGTACCAATGTTTTTAAATAGCGGCTATAGCGGTGTTATAGTGCTATTGCATAGCGGAATTTGAACAAACGGCTATTGTTCCACGATACACTGTTTTGTATAAAATGTTGTGAAATAGCAACTATAGCGGCGCTATGGCAGTGTTGCATGGCGGAATTTGAACAAAACGCTATTTTCTGTAATCTGCAATTGACAACATTGACATGTACATAATTTGTTAACATTGAAATGTACATAATCTGTTTTCAGTTTATTGAAATAAGCTCGCCAAGATAGCTTATGAAAACAACTTATAACATATATGAAAATAATTCGACTTTAGTTCATCTTTTGTTATAAATATAGTTTTTACATAAGTGCTTAACTAAGTTGTTTATCCTGACAGGGCCTTGGCTTTTAGGCCTGACCAAGGTTTCCCTGCTTTAATCAATTTGTATTGTAAAACAGCACACAGAGCAGCAAAATGGTGTGTACTGTGTACAAATTGGACCCTGTGAATGATATATAAATGTATTTCAGTTCTCTTATGAGAATGAAAAATAAAGTCCCTACACAAGTAACGGCGAATCACTCTATAATAAACAGTTTCTTTTCATAAACATAAAGATTCAAATTCAGTTATAAGTAACAGTCCTGTTTGTTAGATTAGATAACTGTAGTCTGTACATGTGTTCAAGTATTGATTTCTTTATGTATGTGGCCATTGGTAACTGATTTTTGAACCCGGATTCCTTACAGTTACGAAATGGTGCAGTAAATGTTTGGCATTGGATGAAGATCAGATGACTTGTATTTTAACTTTATTGAATCAAATTCCAAATACGATTTGAAATCCGAGTTGTCTGATTTTCATCCTACAGTTGCAATTGCGGCTGCTGCAAATGTTGACTGCAGGGTATCCATTTCCCTAACTGTTCCACAACATACTTTTGGTGAAGTTAGAATTTGAGGGCATGGTTCAGCAATGGATGGCATAAAATTTAGAAAATATATCTCTCTGTAACTATTTTTAGTGCATATTTTAATCATAACAAACGTAACAAAATTAATGCTTTGACTTGAATATGTTTTTTGATTTCTTGGTTGTCCTAACTTCCTCTGTCATGTTCTTCTTTAATTTTCAGGAATCCAAAGCTTCAGGCCATTCCAAACCTGCTTAATGATCTCCACATGATATACAAGTTGGAACCGAAGAAGGAACGAAAAGATACACCAGAGAGCTCTGCAAGGGAAGAATTGGAAGGAAACAACAATGATTTCCAACCACTAAAAAAAGTCTTGCCTGTGCTTAATCAGGATCTCAATTTTCTTCCTTATGAAGAAGACGAGGCTGAGTTACTGGATGTTGAGACAAATTTTGAAAGTTCAGCAGGTTTGAAAGTTCACCGGGTTTAGATTCAAATGCTCCCAGTCAATTATCACGTTGTTCCTGCTTTTGAACATTTTATTCCAACAACTCAGTCCTAGCTTTATACACTTATTTTATTAATAGGAATCATGGAGATAAAAAAGTTGTAGTGCAATAAATATGTTGCCAATGCATAACTTCTTCCTAATGTTGAGTTAATTATGTCATGTTTTAACATTTTATATCTAAATTGATAAAGTGAGTGTTTGCAGGTTTTTTAGGAAAGAAAAAGAGGGCAGCCAGTGACCATGTTGCAAAGATTACTTTATCAGATTTGGCCAAGTACTTTGATATGCCAATTGCAGAAGCGTCAAAGAATCTAAATGTTGGGCTTACTGTTCTCAAAAGGAAGTGCAGAGAATTTGGTATTCCTCGCTGGCCTCATAGGAAGATCAAATCACTCGACAGTCTCATTCATGATCTTCAGGTTCTACTCCACTTTTTTATTTATTATTATGACTTTAAGAATCTCTGTTGAGCTATATACATACATACATACATACATACATATCAATTTCCAAGAAAACTTCTATGATTTACAGCCAACTCTTGAACTAATATTTAGATATTCGAACATGACTGTCATTTTAATGTGATTGCAAACCTTATAGTATTTGGTTGAAATACAAACATGAAAATTTATAGTATCTGAGGCTTCTTTAGATAGGTAACCTTATACAAATCTTTTCTGTATTTTTGTTGTCAATTAGCATATGTTTGGATGAGCGAAGAGTTTGATAAAATCACAATGTCACCGTGATTTTGTTTAAGTTACAAAGAGTAGCTTTTGTGCCAAAATCACGGTGGCTCACCGTGATTCGGGTCTCAATGCCAATCCAAACATGCACTGAAGCCTATAAAGTATTTGGTCTATAAAATCTTCGTCTTTCGCTTTATTTTCGTTTATGCTTCACTTTTCCTAACAATCTTCAAGTGAACTCCTTATACACATTGCAACATCTCTGCAGGAAGAGGCAAAACATCAGGAAAGGGAGGATAAAGATGCAGCCATGGCAGTTATAAGAAGGCAAAAGATGTTGGAAAGTGAAAAGGAAAACATAGAGAAGAGGCCCTTCATGGACATACAAATTGAGACCAAGAGATTCAGGCAAGATGTTTTCAAGAGAAGGCACAGAGCCAGAGTTATTGAAAAACAGAATTTTCTACGGTTGGAAGCACCTAAACCTAGCAATTAG
Protein sequence

>MS.gene65320.t1

MRSVHVYRECDGGEKEVEREFVFSENGSYVEMKATPILRLLKSFVSEEIFEGYKNGVWLCVFAFHACNVPQFSHIPTFLLVTRNPKLQAIPNLLNDLHMIYKLEPKKERKDTPESSAREELEGNNNDFQPLKKVLPVLNQDLNFLPYEEDEAELLDVETNFESSAGFLGKKKRAASDHVAKITLSDLAKYFDMPIAEASKNLNVGLTVLKRKCREFGIPRWPHRKIKSLDSLIHDLQEEAKHQEREDKDAAMAVIRRQKMLESEKENIEKRPFMDIQIETKRFRQDVFKRRHRARVIEKQNFLRLEAPKPSN