AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65341

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65341.t1 MTR_5g024390 94.159 428 25 0 1 428 1 428 0.0 833
MS.gene65341.t1 MTR_5g024500 92.991 428 30 0 1 428 1 428 0.0 824
MS.gene65341.t1 MTR_3g103110 68.103 464 108 4 3 428 339 800 0.0 654
MS.gene65341.t1 MTR_7g109640 71.233 438 89 4 3 428 384 796 0.0 644
MS.gene65341.t1 MTR_3g103120 66.970 439 111 3 2 428 425 841 0.0 584
MS.gene65341.t1 MTR_5g071340 43.187 433 207 8 3 421 679 1086 2.28e-113 358
MS.gene65341.t1 MTR_3g464550 43.056 432 209 8 1 419 601 1008 4.79e-109 346
MS.gene65341.t1 MTR_3g103160 39.243 423 163 12 2 421 107 438 7.52e-72 233
MS.gene65341.t1 MTR_2g078040 33.566 429 249 7 3 419 762 1166 1.64e-71 245
MS.gene65341.t1 MTR_1g103830 34.965 429 245 5 3 419 606 1012 8.77e-69 237
MS.gene65341.t1 MTR_1g072130 32.353 408 244 6 3 398 812 1199 1.93e-60 213
MS.gene65341.t1 MTR_7g095140 31.579 304 196 5 125 425 13 307 1.52e-49 172
MS.gene65341.t1 MTR_1g068930 24.946 465 250 15 4 419 441 855 5.23e-40 152
MS.gene65341.t1 MTR_4g099530 27.209 430 258 12 3 419 111 498 5.91e-40 150
MS.gene65341.t1 MTR_1g068930 24.946 465 250 15 4 419 478 892 5.96e-40 152
MS.gene65341.t1 MTR_4g056990 24.274 482 268 15 2 428 189 628 3.16e-31 126
MS.gene65341.t1 MTR_4g057290 25.352 426 213 10 37 394 126 514 4.02e-29 120
MS.gene65341.t1 MTR_4g050090 23.265 490 253 13 12 422 199 644 1.70e-27 115
MS.gene65341.t1 MTR_7g102610 23.729 472 238 17 29 426 527 950 1.19e-22 101
MS.gene65341.t1 MTR_1g062330 22.654 437 268 14 37 421 386 804 1.76e-22 100
MS.gene65341.t1 MTR_6g061000 51.136 88 32 2 90 177 25 101 2.98e-19 82.8
MS.gene65341.t1 MTR_2g084425 30.556 180 116 4 245 423 847 1018 2.34e-17 85.1
MS.gene65341.t1 MTR_2g084425 23.129 294 144 6 7 251 465 725 1.12e-14 77.0
MS.gene65341.t1 MTR_2g084425 23.129 294 144 6 7 251 465 725 1.69e-14 76.3
MS.gene65341.t1 MTR_3g109988 29.891 184 100 6 217 390 191 355 5.33e-13 70.5
MS.gene65341.t1 MTR_3g110005 29.891 184 100 6 217 390 191 355 5.33e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65341.t1 AT5G58610 40.783 434 216 7 3 419 368 777 7.42e-98 311
MS.gene65341.t1 AT5G58610 40.783 434 216 7 3 419 599 1008 1.45e-96 311
MS.gene65341.t1 AT5G58610 40.783 434 216 7 3 419 599 1008 1.45e-96 311
MS.gene65341.t1 AT5G58610 40.625 448 219 9 3 428 590 1012 3.14e-96 311
MS.gene65341.t1 AT5G58610 40.625 448 219 9 3 428 590 1012 4.00e-96 311
MS.gene65341.t1 AT5G58610 40.625 448 219 9 3 428 599 1021 4.17e-96 311
MS.gene65341.t1 AT5G58610 38.902 437 216 8 3 428 590 986 3.12e-86 284
MS.gene65341.t1 AT5G58610 38.902 437 216 8 3 428 590 986 3.37e-86 284
MS.gene65341.t1 AT5G58610 40.576 382 186 7 3 367 590 947 2.06e-81 270
MS.gene65341.t1 AT5G58610 40.576 382 186 7 3 367 599 956 2.38e-81 269
MS.gene65341.t1 AT5G58610 39.826 344 166 7 3 329 590 909 1.89e-67 231
MS.gene65341.t1 AT5G36740 32.401 429 254 6 3 419 545 949 3.46e-67 232
MS.gene65341.t1 AT5G36740 32.401 429 254 6 3 419 545 949 3.46e-67 232
MS.gene65341.t1 AT3G14980 31.640 433 262 6 3 425 621 1029 1.93e-64 224
MS.gene65341.t1 AT3G14980 31.640 433 262 6 3 425 576 984 3.86e-64 223
MS.gene65341.t1 AT1G05380 33.021 427 255 6 3 419 520 925 3.98e-64 223
MS.gene65341.t1 AT1G05380 33.021 427 255 6 3 419 520 925 3.98e-64 223
MS.gene65341.t1 AT1G05380 33.021 427 255 6 3 419 520 925 3.98e-64 223
MS.gene65341.t1 AT3G14980 31.640 433 262 6 3 425 621 1029 5.01e-64 223
MS.gene65341.t1 AT3G14980 31.640 433 262 6 3 425 621 1029 5.01e-64 223
MS.gene65341.t1 AT3G14980 31.640 433 262 6 3 425 621 1029 5.01e-64 223
MS.gene65341.t1 AT5G36740 31.435 439 255 7 3 419 392 806 9.44e-64 221
MS.gene65341.t1 AT5G63900 36.858 331 190 8 94 421 228 542 1.51e-62 211
MS.gene65341.t1 AT4G14920 30.787 432 262 6 3 422 580 986 5.92e-60 211
MS.gene65341.t1 AT4G14920 30.787 432 262 6 3 422 580 986 5.92e-60 211
MS.gene65341.t1 AT4G14920 30.787 432 262 6 3 422 580 986 6.10e-60 211
MS.gene65341.t1 AT5G36670 30.337 445 256 8 3 419 545 963 7.11e-59 208
MS.gene65341.t1 AT5G36670 30.337 445 256 8 3 419 545 963 7.11e-59 208
MS.gene65341.t1 AT5G36740 32.345 371 215 6 3 361 545 891 4.17e-57 202
MS.gene65341.t1 AT5G36740 32.345 371 215 6 3 361 545 891 4.17e-57 202
MS.gene65341.t1 AT5G36670 32.345 371 215 6 3 361 545 891 4.17e-57 202
MS.gene65341.t1 AT5G36670 29.451 455 257 9 3 419 392 820 4.06e-55 197
MS.gene65341.t1 AT5G36670 29.451 455 257 9 3 419 545 973 7.80e-55 196
MS.gene65341.t1 AT3G14980 30.473 338 201 6 3 330 621 934 7.51e-43 161
MS.gene65341.t1 AT2G37520 26.050 476 235 14 4 417 392 812 1.32e-40 154
MS.gene65341.t1 AT3G53680 28.358 469 232 17 4 417 411 830 1.32e-40 154
MS.gene65341.t1 AT3G53680 28.358 469 232 17 4 417 409 828 1.38e-40 154
MS.gene65341.t1 AT2G36720 25.470 479 251 14 13 423 533 973 1.06e-38 149
MS.gene65341.t1 AT2G36720 25.470 479 251 14 13 423 533 973 1.06e-38 149
MS.gene65341.t1 AT2G27980 24.194 496 256 16 6 422 606 1060 5.38e-32 129

Find 68 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATCTGCAACCTTAAT+TGG 0.181951 5.2:-15249204 None:intergenic
TTCACATTCTTGTTTGCATT+TGG 0.183779 5.2:-15249019 None:intergenic
TGGTTTCTATAATGTAATTT+TGG 0.224054 5.2:+15249419 MS.gene65341:CDS
GTTTGTTCAGTTTGTGGTTT+TGG 0.244082 5.2:+15248874 MS.gene65341:CDS
GTGGCAACTATAAGGATTTA+TGG 0.245404 5.2:+15249465 MS.gene65341:CDS
ATCAACTCTCTTCCTGATAA+AGG 0.280267 5.2:-15249345 None:intergenic
TCACATTCTTGTTTGCATTT+GGG 0.312904 5.2:-15249018 None:intergenic
GCATTCCACCTCCGTTGTTT+AGG 0.320877 5.2:+15248931 MS.gene65341:CDS
GTTGATAAAGAATCAAGTTT+TGG 0.324712 5.2:+15248510 MS.gene65341:CDS
GAACGATGGGTATTGTTTCT+TGG 0.326060 5.2:+15248488 None:intergenic
TGATTTCAGTGGCAACTATA+AGG 0.339394 5.2:+15249457 MS.gene65341:CDS
TTATGTCTAAAGAAGTTGTT+AGG 0.347395 5.2:+15249177 MS.gene65341:CDS
AAAGGAGAGTAAGTTGAATA+TGG 0.354999 5.2:+15249281 MS.gene65341:CDS
AATTTAATCACATGGAGAAT+TGG 0.356404 5.2:+15249124 MS.gene65341:CDS
GATAATGTTTGTTCAGTTTG+TGG 0.382559 5.2:+15248868 MS.gene65341:CDS
CTGGGTTCGCATGATCATAT+AGG 0.387880 5.2:+15248739 MS.gene65341:CDS
GGATTGTCAAAGGGAAGCTC+TGG 0.400874 5.2:+15248720 MS.gene65341:CDS
AACACTTGGACAAGATCATT+TGG 0.403595 5.2:+15249636 MS.gene65341:CDS
AAGCACTGAAAAGACCTATA+TGG 0.410177 5.2:+15249742 MS.gene65341:CDS
CGAAGAGGAGTTTAATCAAA+AGG 0.417145 5.2:+15249263 MS.gene65341:CDS
GGCTCTTGGTGTATTGTATG+AGG 0.423950 5.2:+15249302 MS.gene65341:CDS
ACCATCCACAAGTATCTTGT+TGG 0.424383 5.2:+15248678 MS.gene65341:CDS
GTTGTTAGGAAAACCAATTA+AGG 0.435095 5.2:+15249191 MS.gene65341:CDS
CAAACTCGCTTGAACTTTCG+TGG 0.436470 5.2:+15249399 MS.gene65341:CDS
ATTGAACACAAACAAGAATC+TGG 0.437308 5.2:-15249056 None:intergenic
CAAGCAATGCAAGGCCATAT+AGG 0.465235 5.2:-15249756 None:intergenic
CCTGATAAAGGATCAAAAGT+TGG 0.477884 5.2:-15249333 None:intergenic
ACAAGTATCTTGTTGGAAGA+TGG 0.480189 5.2:+15248685 MS.gene65341:CDS
TTGTCTTCGATACTGTTCCT+TGG 0.482098 5.2:-15249520 None:intergenic
ATCGAGTTCCGATTGGTGAA+TGG 0.484059 5.2:+15248959 MS.gene65341:CDS
GGAAGATGGAAGGTCGTTGT+TGG 0.488980 5.2:+15248699 MS.gene65341:CDS
TGGGTTCGCATGATCATATA+GGG 0.493127 5.2:+15248740 MS.gene65341:CDS
GCTCTTGGTGTATTGTATGA+GGG 0.493509 5.2:+15249303 MS.gene65341:CDS
GAGAAACAGTTAACTCGCTT+AGG 0.495020 5.2:+15249573 MS.gene65341:CDS
AGGAATGTGTCATTTGTTGA+TGG 0.496868 5.2:+15249542 MS.gene65341:CDS
CAGAGGATGCTATGAACACT+TGG 0.500216 5.2:+15249622 MS.gene65341:CDS
GTGATTAAATTCAATTCCTA+CGG 0.512914 5.2:-15249112 None:intergenic
GTCGTTGTTGGATTGTCAAA+GGG 0.517227 5.2:+15248711 MS.gene65341:CDS
GGACAGAACCATTCACCAAT+CGG 0.518159 5.2:-15248967 None:intergenic
TGTGAATAGTGTTGACGAAG+AGG 0.521922 5.2:+15249248 MS.gene65341:CDS
TCTTCGATACTGTTCCTTGG+TGG 0.524421 5.2:-15249517 None:intergenic
GGTCGTTGTTGGATTGTCAA+AGG 0.528790 5.2:+15248710 MS.gene65341:CDS
AGTAACAAAGTTGTCAAAAG+AGG 0.532395 5.2:+15248562 MS.gene65341:CDS
AGTAAGTTGAATATGGCTCT+TGG 0.540429 5.2:+15249288 MS.gene65341:CDS
ATCTTCCAACAAGATACTTG+TGG 0.550941 5.2:-15248683 None:intergenic
ATATCACTTTGGATGCGTAA+AGG 0.554258 5.2:+15249089 MS.gene65341:CDS
CACCGGCTTCGAGTCTCATG+CGG 0.565673 5.2:+15248639 MS.gene65341:CDS
ACCGGCTTCGAGTCTCATGC+GGG 0.580291 5.2:+15248640 MS.gene65341:CDS
TGAGACCTAAACAACGGAGG+TGG 0.586505 5.2:-15248936 None:intergenic
GGTCTCAATCGAGTTCCGAT+TGG 0.586950 5.2:+15248952 MS.gene65341:CDS
GATTGTCAAAGGGAAGCTCT+GGG 0.597706 5.2:+15248721 MS.gene65341:CDS
AGGAATTGAATTTAATCACA+TGG 0.598130 5.2:+15249116 MS.gene65341:CDS
TTTCAGTGCTTTGAGACACA+TGG 0.604056 5.2:-15249730 None:intergenic
AGATAATCTAACATGGACAT+TGG 0.607457 5.2:+15249218 MS.gene65341:CDS
CTCCAACATCAAGCAATGCA+AGG 0.609626 5.2:-15249765 None:intergenic
TCCAACAAGATACTTGTGGA+TGG 0.616702 5.2:-15248679 None:intergenic
CAACGGAGGTGGAATGCAGA+AGG 0.622944 5.2:-15248925 None:intergenic
ACCCGCATGAGACTCGAAGC+CGG 0.625739 5.2:-15248641 None:intergenic
GTATCTTGTTGGAAGATGGA+AGG 0.629827 5.2:+15248689 MS.gene65341:CDS
ATTACTTCTGCATTCCTCAG+AGG 0.633654 5.2:+15249605 MS.gene65341:CDS
ACAAGAATGTGAAGATCACA+AGG 0.637763 5.2:+15249029 MS.gene65341:CDS
AATGGGTGAAGTGATTTCAG+TGG 0.642896 5.2:+15249446 MS.gene65341:CDS
TTTGGATGCGTAAAGGCCGT+AGG 0.643030 5.2:+15249096 MS.gene65341:CDS
AGGTTGCAGATAATCTAACA+TGG 0.665981 5.2:+15249211 MS.gene65341:CDS
CTCGATTGAGACCTAAACAA+CGG 0.677857 5.2:-15248942 None:intergenic
AAGGAACAGTATCGAAGACA+AGG 0.678537 5.2:+15249522 MS.gene65341:CDS
AGTGTTCATAGCATCCTCTG+AGG 0.736105 5.2:-15249619 None:intergenic
GATTGAGACCTAAACAACGG+AGG 0.762353 5.2:-15248939 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTAATTTTGGAGAAAAT+GGG + chr5.2:15249429-15249448 MS.gene65341:CDS 20.0%
!!! ACTTTTTTAGAATTTGAGAA+TGG + chr5.2:15249705-15249724 MS.gene65341:CDS 20.0%
!!! TAATGTAATTTTGGAGAAAA+TGG + chr5.2:15249428-15249447 MS.gene65341:CDS 20.0%
!!! TGGTTTCTATAATGTAATTT+TGG + chr5.2:15249419-15249438 MS.gene65341:CDS 20.0%
! AATTTAATCACATGGAGAAT+TGG + chr5.2:15249124-15249143 MS.gene65341:CDS 25.0%
! AGGAATTGAATTTAATCACA+TGG + chr5.2:15249116-15249135 MS.gene65341:CDS 25.0%
! GTGATTAAATTCAATTCCTA+CGG - chr5.2:15249115-15249134 None:intergenic 25.0%
! TTATGTCTAAAGAAGTTGTT+AGG + chr5.2:15249177-15249196 MS.gene65341:CDS 25.0%
!!! GTTGATAAAGAATCAAGTTT+TGG + chr5.2:15248510-15248529 MS.gene65341:CDS 25.0%
AGATAATCTAACATGGACAT+TGG + chr5.2:15249218-15249237 MS.gene65341:CDS 30.0%
AGATTATCTGCAACCTTAAT+TGG - chr5.2:15249207-15249226 None:intergenic 30.0%
AGTAACAAAGTTGTCAAAAG+AGG + chr5.2:15248562-15248581 MS.gene65341:CDS 30.0%
ATTGAACACAAACAAGAATC+TGG - chr5.2:15249059-15249078 None:intergenic 30.0%
GATAATGTTTGTTCAGTTTG+TGG + chr5.2:15248868-15248887 MS.gene65341:CDS 30.0%
GTTGTTAGGAAAACCAATTA+AGG + chr5.2:15249191-15249210 MS.gene65341:CDS 30.0%
TGTGAGCAAAAATATCACTT+TGG + chr5.2:15249078-15249097 MS.gene65341:CDS 30.0%
! AAAGGAGAGTAAGTTGAATA+TGG + chr5.2:15249281-15249300 MS.gene65341:CDS 30.0%
! CATATTTTGCAACAACAAGT+TGG - chr5.2:15248991-15249010 None:intergenic 30.0%
! TCACATTCTTGTTTGCATTT+GGG - chr5.2:15249021-15249040 None:intergenic 30.0%
! TTCACATTCTTGTTTGCATT+TGG - chr5.2:15249022-15249041 None:intergenic 30.0%
!!! AAGAATCAAGTTTTGGTTTC+AGG + chr5.2:15248517-15248536 MS.gene65341:CDS 30.0%
!!! AGAATCAAGTTTTGGTTTCA+GGG + chr5.2:15248518-15248537 MS.gene65341:CDS 30.0%
AACACTTGGACAAGATCATT+TGG + chr5.2:15249636-15249655 MS.gene65341:CDS 35.0%
ACAAGAATGTGAAGATCACA+AGG + chr5.2:15249029-15249048 MS.gene65341:CDS 35.0%
ACAAGTATCTTGTTGGAAGA+TGG + chr5.2:15248685-15248704 MS.gene65341:CDS 35.0%
AGGTTGCAGATAATCTAACA+TGG + chr5.2:15249211-15249230 MS.gene65341:CDS 35.0%
ATATCACTTTGGATGCGTAA+AGG + chr5.2:15249089-15249108 MS.gene65341:CDS 35.0%
ATCAACTCTCTTCCTGATAA+AGG - chr5.2:15249348-15249367 None:intergenic 35.0%
ATCTTCCAACAAGATACTTG+TGG - chr5.2:15248686-15248705 None:intergenic 35.0%
CCTGATAAAGGATCAAAAGT+TGG - chr5.2:15249336-15249355 None:intergenic 35.0%
CGAAGAGGAGTTTAATCAAA+AGG + chr5.2:15249263-15249282 MS.gene65341:CDS 35.0%
GTGGCAACTATAAGGATTTA+TGG + chr5.2:15249465-15249484 MS.gene65341:CDS 35.0%
TGATTTCAGTGGCAACTATA+AGG + chr5.2:15249457-15249476 MS.gene65341:CDS 35.0%
! AGTAAGTTGAATATGGCTCT+TGG + chr5.2:15249288-15249307 MS.gene65341:CDS 35.0%
! CCAACTTTTGATCCTTTATC+AGG + chr5.2:15249333-15249352 MS.gene65341:CDS 35.0%
! GTTTGTTCAGTTTGTGGTTT+TGG + chr5.2:15248874-15248893 MS.gene65341:CDS 35.0%
!! AAGCACTGAAAAGACCTATA+TGG + chr5.2:15249742-15249761 MS.gene65341:CDS 35.0%
!! AGGAATGTGTCATTTGTTGA+TGG + chr5.2:15249542-15249561 MS.gene65341:CDS 35.0%
!!! AGGGACTAATGTTTTTTGTC+AGG + chr5.2:15248537-15248556 MS.gene65341:CDS 35.0%
!!! CAAGATCATTTGGTTTTGCA+AGG + chr5.2:15249646-15249665 MS.gene65341:CDS 35.0%
!!! CGTGTTACTTTTACTATCAC+CGG + chr5.2:15248622-15248641 MS.gene65341:CDS 35.0%
!!! GGGACTAATGTTTTTTGTCA+GGG + chr5.2:15248538-15248557 MS.gene65341:CDS 35.0%
AATGGGTGAAGTGATTTCAG+TGG + chr5.2:15249446-15249465 MS.gene65341:CDS 40.0%
ACCATCCACAAGTATCTTGT+TGG + chr5.2:15248678-15248697 MS.gene65341:CDS 40.0%
ATTACTTCTGCATTCCTCAG+AGG + chr5.2:15249605-15249624 MS.gene65341:CDS 40.0%
CTCGATTGAGACCTAAACAA+CGG - chr5.2:15248945-15248964 None:intergenic 40.0%
GAGAAACAGTTAACTCGCTT+AGG + chr5.2:15249573-15249592 MS.gene65341:CDS 40.0%
GTATCTTGTTGGAAGATGGA+AGG + chr5.2:15248689-15248708 MS.gene65341:CDS 40.0%
GTCGTTGTTGGATTGTCAAA+GGG + chr5.2:15248711-15248730 MS.gene65341:CDS 40.0%
TCCAACAAGATACTTGTGGA+TGG - chr5.2:15248682-15248701 None:intergenic 40.0%
TGGGTTCGCATGATCATATA+GGG + chr5.2:15248740-15248759 MS.gene65341:CDS 40.0%
TTGTCTTCGATACTGTTCCT+TGG - chr5.2:15249523-15249542 None:intergenic 40.0%
! AAGGAACAGTATCGAAGACA+AGG + chr5.2:15249522-15249541 MS.gene65341:CDS 40.0%
! AGTTGCAGAGATAGTTTTCG+TGG + chr5.2:15249494-15249513 MS.gene65341:CDS 40.0%
! GCTCTTGGTGTATTGTATGA+GGG + chr5.2:15249303-15249322 MS.gene65341:CDS 40.0%
!! GGTTTTGCAGTGTAGTTTGT+GGG + chr5.2:15249145-15249164 MS.gene65341:CDS 40.0%
!! TGGTTTTGCAGTGTAGTTTG+TGG + chr5.2:15249144-15249163 MS.gene65341:CDS 40.0%
!! TGTGAATAGTGTTGACGAAG+AGG + chr5.2:15249248-15249267 MS.gene65341:CDS 40.0%
!! TTTCAGTGCTTTGAGACACA+TGG - chr5.2:15249733-15249752 None:intergenic 40.0%
!!! TGTTCAGTTTGTGGTTTTGG+AGG + chr5.2:15248877-15248896 MS.gene65341:CDS 40.0%
AGTGTTCATAGCATCCTCTG+AGG - chr5.2:15249622-15249641 None:intergenic 45.0%
ATCGAGTTCCGATTGGTGAA+TGG + chr5.2:15248959-15248978 MS.gene65341:CDS 45.0%
CAAACTCGCTTGAACTTTCG+TGG + chr5.2:15249399-15249418 MS.gene65341:CDS 45.0%
CAAGCAATGCAAGGCCATAT+AGG - chr5.2:15249759-15249778 None:intergenic 45.0%
CAGAGGATGCTATGAACACT+TGG + chr5.2:15249622-15249641 MS.gene65341:CDS 45.0%
CTGGGTTCGCATGATCATAT+AGG + chr5.2:15248739-15248758 MS.gene65341:CDS 45.0%
GATTGAGACCTAAACAACGG+AGG - chr5.2:15248942-15248961 None:intergenic 45.0%
GATTGTCAAAGGGAAGCTCT+GGG + chr5.2:15248721-15248740 MS.gene65341:CDS 45.0%
GGACAGAACCATTCACCAAT+CGG - chr5.2:15248970-15248989 None:intergenic 45.0%
GGTCGTTGTTGGATTGTCAA+AGG + chr5.2:15248710-15248729 MS.gene65341:CDS 45.0%
TCTTCGATACTGTTCCTTGG+TGG - chr5.2:15249520-15249539 None:intergenic 45.0%
! GGCTCTTGGTGTATTGTATG+AGG + chr5.2:15249302-15249321 MS.gene65341:CDS 45.0%
!!! TTGTGGTTTTGGAGGTGACT+TGG + chr5.2:15248885-15248904 MS.gene65341:CDS 45.0%
GCATTCCACCTCCGTTGTTT+AGG + chr5.2:15248931-15248950 MS.gene65341:CDS 50.0%
GGAAGATGGAAGGTCGTTGT+TGG + chr5.2:15248699-15248718 MS.gene65341:CDS 50.0%
GGATTGTCAAAGGGAAGCTC+TGG + chr5.2:15248720-15248739 MS.gene65341:CDS 50.0%
GGTCTCAATCGAGTTCCGAT+TGG + chr5.2:15248952-15248971 MS.gene65341:CDS 50.0%
TGAGACCTAAACAACGGAGG+TGG - chr5.2:15248939-15248958 None:intergenic 50.0%
TTTGGATGCGTAAAGGCCGT+AGG + chr5.2:15249096-15249115 MS.gene65341:CDS 50.0%
! GATAGTTTTCGTGGCCACCA+AGG + chr5.2:15249503-15249522 MS.gene65341:CDS 50.0%
CAACGGAGGTGGAATGCAGA+AGG - chr5.2:15248928-15248947 None:intergenic 55.0%
ACCCGCATGAGACTCGAAGC+CGG - chr5.2:15248644-15248663 None:intergenic 60.0%
! ACCGGCTTCGAGTCTCATGC+GGG + chr5.2:15248640-15248659 MS.gene65341:CDS 60.0%
!! CACCGGCTTCGAGTCTCATG+CGG + chr5.2:15248639-15248658 MS.gene65341:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 15248493 15249779 15248493 ID=MS.gene65341
chr5.2 mRNA 15248493 15249779 15248493 ID=MS.gene65341.t1;Parent=MS.gene65341
chr5.2 exon 15248493 15249779 15248493 ID=MS.gene65341.t1.exon1;Parent=MS.gene65341.t1
chr5.2 CDS 15248493 15249779 15248493 ID=cds.MS.gene65341.t1;Parent=MS.gene65341.t1
Gene Sequence

>MS.gene65341

ATGGGTATTGTTTCTTGGTTGATAAAGAATCAAGTTTTGGTTTCAGGGACTAATGTTTTTTGTCAGGGTAGTAACAAAGTTGTCAAAAGAGGAAGTTTGTTTTCTGATGCTATAGTCTGTGATTGTTGTCGTGTTACTTTTACTATCACCGGCTTCGAGTCTCATGCGGGTTGTACAAGACATAGACCATCCACAAGTATCTTGTTGGAAGATGGAAGGTCGTTGTTGGATTGTCAAAGGGAAGCTCTGGGTTCGCATGATCATATAGGGAATCATTGTGTTGTCAAAGAAAATCAAAAGAAGAATCATTCTATTGTCAAAGAAAATCGAAAGAAGAATCATTGTGCTGTCAAAGAAAAATCTGAAGCAAATAACGATAATGTTTGTTCAGTTTGTGGTTTTGGAGGTGACTTGGCACTATGCGATCGATGTCCTTCTGCATTCCACCTCCGTTGTTTAGGTCTCAATCGAGTTCCGATTGGTGAATGGTTCTGTCCAACTTGTTGTTGCAAAATATGCTATCGACCCAAATGCAAACAAGAATGTGAAGATCACAAGGATAACCAGATTCTTGTTTGTGTTCAATGTGAGCAAAAATATCACTTTGGATGCGTAAAGGCCGTAGGAATTGAATTTAATCACATGGAGAATTGGTTTTGCAGTGTAGTTTGTGGGAACATGTTCTTATGTCTAAAGAAGTTGTTAGGAAAACCAATTAAGGTTGCAGATAATCTAACATGGACATTGGTGAAGAATGTGAATAGTGTTGACGAAGAGGAGTTTAATCAAAAGGAGAGTAAGTTGAATATGGCTCTTGGTGTATTGTATGAGGGTTTTAATCCAACTTTTGATCCTTTATCAGGAAGAGAGTTGATTAAAGATGTTGTTTTTAGTAGAGAATCAGAACAAACTCGCTTGAACTTTCGTGGTTTCTATAATGTAATTTTGGAGAAAATGGGTGAAGTGATTTCAGTGGCAACTATAAGGATTTATGGTCAAAAAGTTGCAGAGATAGTTTTCGTGGCCACCAAGGAACAGTATCGAAGACAAGGAATGTGTCATTTGTTGATGGATGAGATTGAGAAACAGTTAACTCGCTTAGGAGTTGAGAAATTACTTCTGCATTCCTCAGAGGATGCTATGAACACTTGGACAAGATCATTTGGTTTTGCAAGGATGAATAGTAAAGATAAGTGTCAGTTTATTGATCATACTTTTTTAGAATTTGAGAATGGTACCATGTGTCTCAAAGCACTGAAAAGACCTATATGGCCTTGCATTGCTTGA
Protein sequence

>MS.gene65341.t1

MGIVSWLIKNQVLVSGTNVFCQGSNKVVKRGSLFSDAIVCDCCRVTFTITGFESHAGCTRHRPSTSILLEDGRSLLDCQREALGSHDHIGNHCVVKENQKKNHSIVKENRKKNHCAVKEKSEANNDNVCSVCGFGGDLALCDRCPSAFHLRCLGLNRVPIGEWFCPTCCCKICYRPKCKQECEDHKDNQILVCVQCEQKYHFGCVKAVGIEFNHMENWFCSVVCGNMFLCLKKLLGKPIKVADNLTWTLVKNVNSVDEEEFNQKESKLNMALGVLYEGFNPTFDPLSGRELIKDVVFSRESEQTRLNFRGFYNVILEKMGEVISVATIRIYGQKVAEIVFVATKEQYRRQGMCHLLMDEIEKQLTRLGVEKLLLHSSEDAMNTWTRSFGFARMNSKDKCQFIDHTFLEFENGTMCLKALKRPIWPCIA