AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65416

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65416.t1 MTR_4g007490 95.000 380 12 1 1 380 1 373 0.0 728
MS.gene65416.t1 MTR_3g450500 67.454 381 117 5 1 380 1 375 3.86e-178 500
MS.gene65416.t1 MTR_2g019840 38.000 350 203 6 32 378 34 372 8.80e-69 221
MS.gene65416.t1 MTR_2g019810 36.885 366 219 5 14 378 6 360 2.12e-66 215
MS.gene65416.t1 MTR_4g119550 34.835 333 209 5 45 375 52 378 5.31e-59 196
MS.gene65416.t1 MTR_4g120380 31.909 351 225 5 27 374 30 369 1.96e-56 189
MS.gene65416.t1 MTR_2g437260 29.851 335 226 3 43 374 35 363 1.35e-53 182
MS.gene65416.t1 MTR_2g437030 33.148 359 226 7 22 375 105 454 1.73e-53 184
MS.gene65416.t1 MTR_4g120380 32.229 332 211 5 27 355 30 350 2.32e-53 182
MS.gene65416.t1 MTR_4g119580 32.877 365 225 6 22 379 23 374 2.30e-52 179
MS.gene65416.t1 MTR_2g437040 33.141 347 223 5 32 375 28 368 4.96e-52 178
MS.gene65416.t1 MTR_2g436460 33.724 341 216 5 36 374 41 373 1.20e-51 177
MS.gene65416.t1 MTR_2g436400 30.939 362 240 6 17 375 21 375 5.44e-50 172
MS.gene65416.t1 MTR_2g437130 32.011 378 243 8 1 375 3 369 2.46e-49 171
MS.gene65416.t1 MTR_2g437080 30.811 370 245 6 11 375 5 368 6.54e-49 169
MS.gene65416.t1 MTR_2g437160 31.339 351 229 8 29 375 51 393 2.83e-48 169
MS.gene65416.t1 MTR_2g437020 32.493 357 221 9 13 360 9 354 4.34e-48 167
MS.gene65416.t1 MTR_2g437150 31.405 363 237 7 22 375 29 388 8.74e-48 167
MS.gene65416.t1 MTR_2g437060 31.026 390 232 9 10 375 7 383 6.81e-47 164
MS.gene65416.t1 MTR_2g436440 30.704 355 234 6 24 375 29 374 8.61e-47 164
MS.gene65416.t1 MTR_2g437240 29.067 375 223 5 39 375 27 396 8.06e-46 162
MS.gene65416.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 7.28e-45 159
MS.gene65416.t1 MTR_2g437120 29.805 359 241 6 22 375 29 381 8.82e-45 159
MS.gene65416.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 9.01e-45 160
MS.gene65416.t1 MTR_2g437170 30.028 363 242 7 22 375 29 388 5.13e-44 157
MS.gene65416.t1 MTR_2g437100 29.288 379 252 8 15 380 23 398 1.23e-43 156
MS.gene65416.t1 MTR_2g437180 30.422 332 223 5 46 374 51 377 2.29e-43 155
MS.gene65416.t1 MTR_2g437200 28.947 380 225 8 35 375 23 396 5.14e-43 154
MS.gene65416.t1 MTR_2g437990 29.973 367 246 6 15 374 17 379 4.58e-42 152
MS.gene65416.t1 MTR_2g438010 29.620 368 247 8 15 374 37 400 6.80e-42 152
MS.gene65416.t1 MTR_2g076320 30.707 368 243 8 15 374 17 380 7.61e-42 151
MS.gene65416.t1 MTR_2g438020 27.596 366 226 3 46 375 73 435 3.35e-39 146
MS.gene65416.t1 MTR_8g081000 25.513 341 249 3 37 375 48 385 2.82e-32 125
MS.gene65416.t1 MTR_8g081000 25.513 341 249 3 37 375 48 385 3.41e-32 125
MS.gene65416.t1 MTR_8g081000 25.513 341 249 3 37 375 48 385 3.82e-32 125
MS.gene65416.t1 MTR_8g080990 26.724 348 246 4 37 380 35 377 5.27e-30 119
MS.gene65416.t1 MTR_2g437940 26.866 335 207 7 46 375 51 352 1.56e-28 115
MS.gene65416.t1 MTR_4g119570 28.205 234 129 3 138 371 31 225 9.17e-24 98.6
MS.gene65416.t1 MTR_2g436370 37.143 175 103 5 27 198 30 200 2.67e-21 91.3
MS.gene65416.t1 MTR_2g437090 29.605 152 107 0 224 375 5 156 6.32e-21 89.4
MS.gene65416.t1 MTR_2g437160 28.767 219 135 6 177 375 85 302 2.04e-20 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65416.t1 AT5G07900 40.882 340 198 3 37 374 61 399 1.05e-86 268
MS.gene65416.t1 AT1G21150 33.423 371 240 4 10 374 17 386 3.53e-59 197
MS.gene65416.t1 AT1G21150 33.423 371 240 4 10 374 81 450 1.09e-58 197
MS.gene65416.t1 AT1G21150 34.000 350 222 4 33 374 2 350 1.78e-57 191
MS.gene65416.t1 AT1G62120 30.179 391 226 7 14 360 18 405 8.41e-43 155
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61970 28.729 362 206 7 42 361 49 400 8.69e-39 144
MS.gene65416.t1 AT1G61980 29.412 357 210 5 41 360 48 399 1.02e-38 143
MS.gene65416.t1 AT1G61980 29.412 357 210 5 41 360 48 399 1.02e-38 143
MS.gene65416.t1 AT1G61980 29.412 357 210 5 41 360 48 399 1.02e-38 143
MS.gene65416.t1 AT1G61990 28.052 385 226 6 27 371 32 405 2.45e-38 142
MS.gene65416.t1 AT1G61990 28.052 385 226 6 27 371 32 405 2.45e-38 142
MS.gene65416.t1 AT3G46950 32.226 301 188 6 14 309 18 307 1.75e-37 140
MS.gene65416.t1 AT1G62010 29.558 362 191 9 42 361 45 384 2.44e-36 137
MS.gene65416.t1 AT5G23930 29.120 443 222 10 7 370 12 441 9.42e-36 136
MS.gene65416.t1 AT1G62150 31.900 279 175 5 35 307 48 317 9.17e-34 130
MS.gene65416.t1 AT1G62085 32.248 307 185 8 14 307 18 314 2.06e-32 127
MS.gene65416.t1 AT1G62085 32.248 307 185 8 14 307 18 314 2.06e-32 127
MS.gene65416.t1 AT1G61960 33.577 274 173 6 27 296 32 300 3.52e-31 123
MS.gene65416.t1 AT1G62110 31.633 294 174 7 27 307 32 311 4.08e-31 123
MS.gene65416.t1 AT5G64950 29.221 308 207 5 77 375 71 376 1.24e-30 121
MS.gene65416.t1 AT1G56380 23.433 367 230 12 39 361 10 369 1.39e-17 84.0
MS.gene65416.t1 AT1G56380 23.433 367 230 12 39 361 21 380 1.46e-17 84.0
MS.gene65416.t1 AT1G62490 27.715 267 149 7 30 296 83 305 9.68e-15 75.1

Find 67 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACCTTGTTGAAAGTTTA+AGG 0.085452 4.3:+87924030 None:intergenic
GAAAGATTTGCCATTGTTAT+TGG 0.156741 4.3:-87923124 MS.gene65416:CDS
CTTGTCACCAATCAAGATTT+TGG 0.169173 4.3:+87923824 None:intergenic
TAAGTATGGACAAAGAAATT+TGG 0.197332 4.3:-87923476 MS.gene65416:CDS
TATGAAAACAATGGACTTCT+TGG 0.234883 4.3:-87923349 MS.gene65416:CDS
GAATGCTACACAAGGAAAAT+TGG 0.258902 4.3:+87923596 None:intergenic
AAAAGTTGAATAACAGCATT+TGG 0.293808 4.3:+87923987 None:intergenic
TTCCTCACTTGTCATAATAA+AGG 0.304109 4.3:+87923179 None:intergenic
AAAGAACAGTAGTTGAATAA+AGG 0.305931 4.3:+87924171 None:intergenic
AATGGCAAATCTTTCTGAAA+TGG 0.311445 4.3:+87923132 None:intergenic
ATAACAGCATTTGGGCCATT+AGG 0.321633 4.3:+87923996 None:intergenic
AAGTATGGACAAAGAAATTT+GGG 0.343578 4.3:-87923475 MS.gene65416:CDS
GATTTCCCCAAAATCTTGAT+TGG 0.348520 4.3:-87923831 MS.gene65416:CDS
TAACAGCATTTGGGCCATTA+GGG 0.351944 4.3:+87923997 None:intergenic
GGGCATGTAGCAATGTCTTC+TGG 0.354285 4.3:+87923306 None:intergenic
TCAAAGTTCCATTTCCAGTT+TGG 0.357697 4.3:-87923622 MS.gene65416:CDS
TCAAGCCTCATAAACTCATC+TGG 0.358233 4.3:-87924071 MS.gene65416:CDS
ATTGGATACCAAACTGGAAA+TGG 0.363470 4.3:+87923614 None:intergenic
GTTAATTCAGTAAAAGAAAT+GGG 0.366519 4.3:-87923546 MS.gene65416:CDS
TCACATACTCAGGATACTTT+AGG 0.366731 4.3:+87923391 None:intergenic
ATGGCAAATCTTTCTGAAAT+GGG 0.369413 4.3:+87923133 None:intergenic
CAACCTTGTTGAAAGTTTAA+GGG 0.375048 4.3:+87924031 None:intergenic
ATCTTGATAACAGCACATTT+AGG 0.385800 4.3:+87923246 None:intergenic
AGGAAAATTGGATACCAAAC+TGG 0.392789 4.3:+87923608 None:intergenic
TGTTAATTCAGTAAAAGAAA+TGG 0.415470 4.3:-87923547 MS.gene65416:CDS
AAGTGTCTTCTCCAAGTTGC+GGG 0.424163 4.3:+87923272 None:intergenic
TAGCTTTGAGGCGTGGATCA+TGG 0.426891 4.3:-87923716 MS.gene65416:CDS
AAGAACAGTAGTTGAATAAA+GGG 0.427774 4.3:+87924172 None:intergenic
TCCAACACAAATCAACATAA+AGG 0.430035 4.3:-87924110 MS.gene65416:CDS
GATTCGAGTAATTGTAAGTA+TGG 0.436929 4.3:-87923490 MS.gene65416:CDS
TTCCTCAAAACCTCAATGTT+CGG 0.445259 4.3:+87923657 None:intergenic
CTCAACAAATCTAGAATGCT+TGG 0.457146 4.3:+87923572 None:intergenic
ACCTTTATGTTGATTTGTGT+TGG 0.460913 4.3:+87924109 None:intergenic
AAGTTGCGGGTCAGAATCCC+AGG 0.461038 4.3:+87923285 None:intergenic
CCTTCTTATCATCGCGAACT+AGG 0.464714 4.3:+87923745 None:intergenic
AACAGCAGAGAAAAGTGAAA+AGG 0.467691 4.3:+87924133 None:intergenic
TCAAGAGGTTTAATAAAGAG+TGG 0.472917 4.3:-87923216 MS.gene65416:CDS
GAAGACATTGCTACATGCCC+TGG 0.473610 4.3:-87923303 MS.gene65416:CDS
AAAGTTGAATAACAGCATTT+GGG 0.476726 4.3:+87923988 None:intergenic
CAAAAGGGTGTTTCTTAACA+AGG 0.481935 4.3:+87923928 None:intergenic
TTGCAACTCATAAACCCTAA+TGG 0.484587 4.3:-87924011 MS.gene65416:CDS
ACAACCCAGATGAGTTTATG+AGG 0.490035 4.3:+87924066 None:intergenic
GGACTTCTTGGTGAAGGATG+TGG 0.492155 4.3:-87923337 MS.gene65416:CDS
ACAGCAGAGAAAAGTGAAAA+GGG 0.500458 4.3:+87924134 None:intergenic
TGGGATTCTGACCCGCAACT+TGG 0.503137 4.3:-87923283 MS.gene65416:CDS
CCTAGTTCGCGATGATAAGA+AGG 0.518018 4.3:-87923745 MS.gene65416:CDS
ATCAATGGTTGAAGCCCCAA+TGG 0.538102 4.3:+87923851 None:intergenic
CGATTCTGTTCCGAACATTG+AGG 0.538376 4.3:-87923667 MS.gene65416:CDS
CAAGCATTCTAGATTTGTTG+AGG 0.552143 4.3:-87923571 MS.gene65416:CDS
TATGAGGCTTGACAATGTAA+AGG 0.554394 4.3:+87924082 None:intergenic
GACTTCTTGGTGAAGGATGT+GGG 0.555487 4.3:-87923336 MS.gene65416:CDS
CAAGCCTCATAAACTCATCT+GGG 0.559442 4.3:-87924070 MS.gene65416:CDS
GCAATGAGAAAATGATACAT+TGG 0.568531 4.3:+87924218 None:intergenic
AAAACAAAATACAACTTCGA+AGG 0.568698 4.3:+87923516 None:intergenic
TCTGAGATCATCACATACTC+AGG 0.569253 4.3:+87923381 None:intergenic
AACAATGGACTTCTTGGTGA+AGG 0.587900 4.3:-87923343 MS.gene65416:CDS
AAGACATTGCTACATGCCCT+GGG 0.601471 4.3:-87923302 MS.gene65416:CDS
CAAGTGTCTTCTCCAAGTTG+CGG 0.602943 4.3:+87923271 None:intergenic
TACCTTTATTATGACAAGTG+AGG 0.612836 4.3:-87923181 MS.gene65416:CDS
AAACTGGAAATGGAACTTTG+AGG 0.616422 4.3:+87923624 None:intergenic
TGGCAAATCTTTCTGAAATG+GGG 0.627523 4.3:+87923134 None:intergenic
TTATATGCATCCAATAACAA+TGG 0.629215 4.3:+87923114 None:intergenic
TTAATTCAGTAAAAGAAATG+GGG 0.635626 4.3:-87923545 MS.gene65416:CDS
AAGCCCTTAAACTTTCAACA+AGG 0.642886 4.3:-87924034 MS.gene65416:CDS
CGGAACAGAATCGTTAACCA+TGG 0.656192 4.3:+87923677 None:intergenic
TGCTTGGTGAATGCTACACA+AGG 0.683625 4.3:+87923588 None:intergenic
AGTTGCGGGTCAGAATCCCA+GGG 0.702455 4.3:+87923286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAATTATGAAAACAA+TGG - chr4.3:87923952-87923971 MS.gene65416:CDS 15.0%
!!! AATTTTGATAAAAGTACAAA+AGG + chr4.3:87923401-87923420 None:intergenic 15.0%
!!! ATTTTGATAAAAGTACAAAA+GGG + chr4.3:87923400-87923419 None:intergenic 15.0%
!! GTTAATTCAGTAAAAGAAAT+GGG - chr4.3:87923764-87923783 MS.gene65416:CDS 20.0%
!! TGTTAATTCAGTAAAAGAAA+TGG - chr4.3:87923763-87923782 MS.gene65416:CDS 20.0%
!! TTAATTCAGTAAAAGAAATG+GGG - chr4.3:87923765-87923784 MS.gene65416:CDS 20.0%
! AAAACAAAATACAACTTCGA+AGG + chr4.3:87923797-87923816 None:intergenic 25.0%
! AAAAGTTGAATAACAGCATT+TGG + chr4.3:87923326-87923345 None:intergenic 25.0%
! AAAGAACAGTAGTTGAATAA+AGG + chr4.3:87923142-87923161 None:intergenic 25.0%
! AAAGTTGAATAACAGCATTT+GGG + chr4.3:87923325-87923344 None:intergenic 25.0%
! AAGAACAGTAGTTGAATAAA+GGG + chr4.3:87923141-87923160 None:intergenic 25.0%
! AAGTATGGACAAAGAAATTT+GGG - chr4.3:87923835-87923854 MS.gene65416:CDS 25.0%
! TAAGTATGGACAAAGAAATT+TGG - chr4.3:87923834-87923853 MS.gene65416:CDS 25.0%
! TTATATGCATCCAATAACAA+TGG + chr4.3:87924199-87924218 None:intergenic 25.0%
!!! CAAAATTTAAGATTTTCGAG+AGG - chr4.3:87923861-87923880 MS.gene65416:CDS 25.0%
!!! TTCGAAGTTGTATTTTGTTT+TGG - chr4.3:87923796-87923815 MS.gene65416:CDS 25.0%
CAACCTTGTTGAAAGTTTAA+GGG + chr4.3:87923282-87923301 None:intergenic 30.0%
GAAAGATTTGCCATTGTTAT+TGG - chr4.3:87924186-87924205 MS.gene65416:CDS 30.0%
GATTCGAGTAATTGTAAGTA+TGG - chr4.3:87923820-87923839 MS.gene65416:CDS 30.0%
TACCTTTATTATGACAAGTG+AGG - chr4.3:87924129-87924148 MS.gene65416:CDS 30.0%
TCAAGAGGTTTAATAAAGAG+TGG - chr4.3:87924094-87924113 MS.gene65416:CDS 30.0%
TCCAACACAAATCAACATAA+AGG - chr4.3:87923200-87923219 MS.gene65416:CDS 30.0%
TTCCTCACTTGTCATAATAA+AGG + chr4.3:87924134-87924153 None:intergenic 30.0%
! AATGGCAAATCTTTCTGAAA+TGG + chr4.3:87924181-87924200 None:intergenic 30.0%
! ATCTTGATAACAGCACATTT+AGG + chr4.3:87924067-87924086 None:intergenic 30.0%
! ATTCAACTTTTCAGAAGCTA+TGG - chr4.3:87923335-87923354 MS.gene65416:CDS 30.0%
! GAACTTTTACAATGATTCCA+TGG - chr4.3:87923616-87923635 MS.gene65416:CDS 30.0%
! GATTGCAAAAACTTGAGTTT+TGG + chr4.3:87923440-87923459 None:intergenic 30.0%
! TATGAAAACAATGGACTTCT+TGG - chr4.3:87923961-87923980 MS.gene65416:CDS 30.0%
! TCAAGTTTTTGCAATCCATT+GGG - chr4.3:87923444-87923463 MS.gene65416:CDS 30.0%
! TTGTCACCAATCAAGATTTT+GGG + chr4.3:87923488-87923507 None:intergenic 30.0%
!! AAAACTTGAGTTTTGGCAAA+AGG + chr4.3:87923433-87923452 None:intergenic 30.0%
!! AAACTTGAGTTTTGGCAAAA+GGG + chr4.3:87923432-87923451 None:intergenic 30.0%
!! AATTTAAGATTTTCGAGAGG+TGG - chr4.3:87923864-87923883 MS.gene65416:CDS 30.0%
!! ACCTTTATGTTGATTTGTGT+TGG + chr4.3:87923204-87923223 None:intergenic 30.0%
!! ATGGCAAATCTTTCTGAAAT+GGG + chr4.3:87924180-87924199 None:intergenic 30.0%
!! ATTTAAGATTTTCGAGAGGT+GGG - chr4.3:87923865-87923884 MS.gene65416:CDS 30.0%
!! CTTTTAAGAATCTTGTAACG+AGG + chr4.3:87923545-87923564 None:intergenic 30.0%
!!! AAAGTTTAAGGGCTTTTTCA+GGG + chr4.3:87923271-87923290 None:intergenic 30.0%
!!! ATCAAGATTTTGAAGTCAAG+AGG - chr4.3:87924079-87924098 MS.gene65416:CDS 30.0%
AAACTGGAAATGGAACTTTG+AGG + chr4.3:87923689-87923708 None:intergenic 35.0%
AACAGCAGAGAAAAGTGAAA+AGG + chr4.3:87923180-87923199 None:intergenic 35.0%
AAGCCCTTAAACTTTCAACA+AGG - chr4.3:87923276-87923295 MS.gene65416:CDS 35.0%
ACAGCAGAGAAAAGTGAAAA+GGG + chr4.3:87923179-87923198 None:intergenic 35.0%
AGGAAAATTGGATACCAAAC+TGG + chr4.3:87923705-87923724 None:intergenic 35.0%
ATTGGATACCAAACTGGAAA+TGG + chr4.3:87923699-87923718 None:intergenic 35.0%
GAATGCTACACAAGGAAAAT+TGG + chr4.3:87923717-87923736 None:intergenic 35.0%
GATTTCCCCAAAATCTTGAT+TGG - chr4.3:87923479-87923498 MS.gene65416:CDS 35.0%
GCAACCTTGTTGAAAGTTTA+AGG + chr4.3:87923283-87923302 None:intergenic 35.0%
TATGAGGCTTGACAATGTAA+AGG + chr4.3:87923231-87923250 None:intergenic 35.0%
TCAAAGTTCCATTTCCAGTT+TGG - chr4.3:87923688-87923707 MS.gene65416:CDS 35.0%
TCACATACTCAGGATACTTT+AGG + chr4.3:87923922-87923941 None:intergenic 35.0%
TTCCTCAAAACCTCAATGTT+CGG + chr4.3:87923656-87923675 None:intergenic 35.0%
TTGCAACTCATAAACCCTAA+TGG - chr4.3:87923299-87923318 MS.gene65416:CDS 35.0%
! CAAGTTTTTGCAATCCATTG+GGG - chr4.3:87923445-87923464 MS.gene65416:CDS 35.0%
! CTCAACAAATCTAGAATGCT+TGG + chr4.3:87923741-87923760 None:intergenic 35.0%
! CTCAAGTTTTTGCAATCCAT+TGG - chr4.3:87923443-87923462 MS.gene65416:CDS 35.0%
! CTTGTCACCAATCAAGATTT+TGG + chr4.3:87923489-87923508 None:intergenic 35.0%
! TGTCACCAATCAAGATTTTG+GGG + chr4.3:87923487-87923506 None:intergenic 35.0%
!! CAAAAGGGTGTTTCTTAACA+AGG + chr4.3:87923385-87923404 None:intergenic 35.0%
!! CAAGATTTTGGGGAAATCAA+TGG + chr4.3:87923477-87923496 None:intergenic 35.0%
!! CAAGCATTCTAGATTTGTTG+AGG - chr4.3:87923739-87923758 MS.gene65416:CDS 35.0%
!! GAAAGTTTAAGGGCTTTTTC+AGG + chr4.3:87923272-87923291 None:intergenic 35.0%
!! TGGCAAATCTTTCTGAAATG+GGG + chr4.3:87924179-87924198 None:intergenic 35.0%
!! TTTAAGATTTTCGAGAGGTG+GGG - chr4.3:87923866-87923885 MS.gene65416:CDS 35.0%
!!! AAGTTTAAGGGCTTTTTCAG+GGG + chr4.3:87923270-87923289 None:intergenic 35.0%
!!! AGAAGGTAGTTTTAGCTTTG+AGG - chr4.3:87923582-87923601 MS.gene65416:CDS 35.0%
ACAACCCAGATGAGTTTATG+AGG + chr4.3:87923247-87923266 None:intergenic 40.0%
ATAACAGCATTTGGGCCATT+AGG + chr4.3:87923317-87923336 None:intergenic 40.0%
CAAGCCTCATAAACTCATCT+GGG - chr4.3:87923240-87923259 MS.gene65416:CDS 40.0%
TAACAGCATTTGGGCCATTA+GGG + chr4.3:87923316-87923335 None:intergenic 40.0%
TCAAGCCTCATAAACTCATC+TGG - chr4.3:87923239-87923258 MS.gene65416:CDS 40.0%
! AACAATGGACTTCTTGGTGA+AGG - chr4.3:87923967-87923986 MS.gene65416:CDS 40.0%
! CAAGTTTTTGACAGCAAGCT+TGG - chr4.3:87923505-87923524 MS.gene65416:CDS 40.0%
! TCTGAGATCATCACATACTC+AGG + chr4.3:87923932-87923951 None:intergenic 40.0%
! TTCCGAACATTGAGGTTTTG+AGG - chr4.3:87923651-87923670 MS.gene65416:CDS 40.0%
!!! ATTGAGGTTTTGAGGAAGCT+TGG - chr4.3:87923659-87923678 MS.gene65416:CDS 40.0%
AAGACATTGCTACATGCCCT+GGG - chr4.3:87924008-87924027 MS.gene65416:CDS 45.0%
AAGTGTCTTCTCCAAGTTGC+GGG + chr4.3:87924041-87924060 None:intergenic 45.0%
ATCAATGGTTGAAGCCCCAA+TGG + chr4.3:87923462-87923481 None:intergenic 45.0%
CAAGTGTCTTCTCCAAGTTG+CGG + chr4.3:87924042-87924061 None:intergenic 45.0%
CCTAGTTCGCGATGATAAGA+AGG - chr4.3:87923565-87923584 MS.gene65416:CDS 45.0%
CCTTCTTATCATCGCGAACT+AGG + chr4.3:87923568-87923587 None:intergenic 45.0%
CGATTCTGTTCCGAACATTG+AGG - chr4.3:87923643-87923662 MS.gene65416:CDS 45.0%
CGGAACAGAATCGTTAACCA+TGG + chr4.3:87923636-87923655 None:intergenic 45.0%
GACTTCTTGGTGAAGGATGT+GGG - chr4.3:87923974-87923993 MS.gene65416:CDS 45.0%
! TGCTTGGTGAATGCTACACA+AGG + chr4.3:87923725-87923744 None:intergenic 45.0%
!!! GTAGTTTTAGCTTTGAGGCG+TGG - chr4.3:87923587-87923606 MS.gene65416:CDS 45.0%
GAAGACATTGCTACATGCCC+TGG - chr4.3:87924007-87924026 MS.gene65416:CDS 50.0%
GGACTTCTTGGTGAAGGATG+TGG - chr4.3:87923973-87923992 MS.gene65416:CDS 50.0%
GGGCATGTAGCAATGTCTTC+TGG + chr4.3:87924007-87924026 None:intergenic 50.0%
!! TAGCTTTGAGGCGTGGATCA+TGG - chr4.3:87923594-87923613 MS.gene65416:CDS 50.0%
AAGTTGCGGGTCAGAATCCC+AGG + chr4.3:87924028-87924047 None:intergenic 55.0%
AGTTGCGGGTCAGAATCCCA+GGG + chr4.3:87924027-87924046 None:intergenic 55.0%
TGGGATTCTGACCCGCAACT+TGG - chr4.3:87924027-87924046 MS.gene65416:CDS 55.0%
!! AGATTTTCGAGAGGTGGGGC+TGG - chr4.3:87923870-87923889 MS.gene65416:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 87923095 87924237 87923095 ID=MS.gene65416
chr4.3 mRNA 87923095 87924237 87923095 ID=MS.gene65416.t1;Parent=MS.gene65416
chr4.3 exon 87923095 87924237 87923095 ID=MS.gene65416.t1.exon1;Parent=MS.gene65416.t1
chr4.3 CDS 87923095 87924237 87923095 ID=cds.MS.gene65416.t1;Parent=MS.gene65416.t1
Gene Sequence

>MS.gene65416

ATGTATCATTTTCTCATTGCTAGATTCACTCATACTCATAGAACCCTTTATTCAACTACTGTTCTTTATCACAGACACAATCCCTTTTCACTTTTCTCTGCTGTTTCCAACACAAATCAACATAAAGGTGACACCTTTACATTGTCAAGCCTCATAAACTCATCTGGGTTGTCCCCTGAAAAAGCCCTTAAACTTTCAACAAGGTTGCAACTCATAAACCCTAATGGCCCAAATGCTGTTATTCAACTTTTCAGAAGCTATGGTTTCTCTGATTCTCAGTTATCAAGCCTTGTTAAGAAACACCCTTTTGTACTTTTATCAAAATTTGATAAGACCCTTTTGCCAAAACTCAAGTTTTTGCAATCCATTGGGGCTTCAACCATTGATTTCCCCAAAATCTTGATTGGTGACAAGTTTTTGACAGCAAGCTTGGAGAAAACGATAATACCTCGTTACAAGATTCTTAAAAGCCTAGTTCGCGATGATAAGAAGGTAGTTTTAGCTTTGAGGCGTGGATCATGGAACTTTTACAATGATTCCATGGTTAACGATTCTGTTCCGAACATTGAGGTTTTGAGGAAGCTTGGTGTGCCTCAAAGTTCCATTTCCAGTTTGGTATCCAATTTTCCTTGTGTAGCATTCACCAAGCATTCTAGATTTGTTGAGGCTGTTAATTCAGTAAAAGAAATGGGGTTCAATCCTTCGAAGTTGTATTTTGTTTTGGCGATTCGAGTAATTGTAAGTATGGACAAAGAAATTTGGGAATCAAAATTTAAGATTTTCGAGAGGTGGGGCTGGTCGAGAGATATTTGTCGTTTCGCTTTCCTAAAGTATCCTGAGTATGTGATGATCTCAGAGAAAAAAATTATGAAAACAATGGACTTCTTGGTGAAGGATGTGGGTCTTACACCAGAAGACATTGCTACATGCCCTGGGATTCTGACCCGCAACTTGGAGAAGACACTTGTACCTAAATGTGCTGTTATCAAGATTTTGAAGTCAAGAGGTTTAATAAAGAGTGGTTTGCGTGTAAGTACCTTTATTATGACAAGTGAGGAAAAGTTTGTGCAGAAATATGTGACCCCATTTCAGAAAGATTTGCCATTGTTATTGGATGCATATAAAGTTCAGAAATCAGATTGA
Protein sequence

>MS.gene65416.t1

MYHFLIARFTHTHRTLYSTTVLYHRHNPFSLFSAVSNTNQHKGDTFTLSSLINSSGLSPEKALKLSTRLQLINPNGPNAVIQLFRSYGFSDSQLSSLVKKHPFVLLSKFDKTLLPKLKFLQSIGASTIDFPKILIGDKFLTASLEKTIIPRYKILKSLVRDDKKVVLALRRGSWNFYNDSMVNDSVPNIEVLRKLGVPQSSISSLVSNFPCVAFTKHSRFVEAVNSVKEMGFNPSKLYFVLAIRVIVSMDKEIWESKFKIFERWGWSRDICRFAFLKYPEYVMISEKKIMKTMDFLVKDVGLTPEDIATCPGILTRNLEKTLVPKCAVIKILKSRGLIKSGLRVSTFIMTSEEKFVQKYVTPFQKDLPLLLDAYKVQKSD