Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65450.t1 | TYH09395.1 | 51.8 | 83 | 39 | 1 | 1 | 82 | 162 | 244 | 4.40E-09 | 70.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65450.t1 | Q84TQ7 | 58.1 | 62 | 26 | 0 | 1 | 62 | 162 | 223 | 4.1e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65450.t1 | A0A5D2FTP1 | 51.8 | 83 | 39 | 1 | 1 | 82 | 162 | 244 | 3.2e-09 | 70.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene65450.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65450.t1 | MTR_4g122240 | 62.121 | 66 | 24 | 1 | 1 | 66 | 24 | 88 | 1.24e-18 | 75.1 |
| MS.gene65450.t1 | MTR_3g065980 | 59.677 | 62 | 25 | 0 | 1 | 62 | 160 | 221 | 2.52e-17 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65450.t1 | AT1G66350 | 48.052 | 77 | 36 | 2 | 1 | 73 | 143 | 219 | 4.30e-15 | 70.1 |
| MS.gene65450.t1 | AT5G17490 | 55.932 | 59 | 26 | 0 | 4 | 62 | 151 | 209 | 1.55e-14 | 68.6 |
| MS.gene65450.t1 | AT2G01570 | 54.839 | 62 | 28 | 0 | 1 | 62 | 212 | 273 | 1.72e-14 | 68.6 |
| MS.gene65450.t1 | AT1G14920 | 51.613 | 62 | 30 | 0 | 1 | 62 | 160 | 221 | 1.91e-14 | 68.2 |
| MS.gene65450.t1 | AT3G03450 | 51.613 | 62 | 30 | 0 | 1 | 62 | 171 | 232 | 3.37e-14 | 67.4 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTCCTCCGGAGGGGAGG+TGG | 0.265974 | 1.1:-25673496 | None:intergenic |
| AGGGGAGGTGGGGTGGTGGC+TGG | 0.331095 | 1.1:-25673484 | None:intergenic |
| CCGGAGGGGAGGTGGGGTGG+TGG | 0.362501 | 1.1:-25673488 | None:intergenic |
| CCTCCGGAGGGGAGGTGGGG+TGG | 0.372000 | 1.1:-25673491 | None:intergenic |
| CCTTCCTCCGGAGGGGAGGT+GGG | 0.394394 | 1.1:-25673495 | None:intergenic |
| AGTTCAACCCCTATCGCCTC+TGG | 0.395762 | 1.1:-25673250 | None:intergenic |
| CTTCCTCCGGAGGGGAGGTG+GGG | 0.401814 | 1.1:-25673494 | None:intergenic |
| CCCACCTCCCCTCCGGAGGA+AGG | 0.419340 | 1.1:+25673495 | MS.gene65450:CDS |
| GTCATCACCCCTCCGGATGA+AGG | 0.420810 | 1.1:+25673525 | MS.gene65450:CDS |
| ATGACTGGCGGCCCTTCCTC+CGG | 0.437056 | 1.1:-25673507 | None:intergenic |
| GAGACAGTGACTGGCCTTGT+AGG | 0.440020 | 1.1:-25673549 | None:intergenic |
| CCATGGACCCAGAGGCGATA+GGG | 0.455974 | 1.1:+25673242 | None:intergenic |
| CCCTATCGCCTCTGGGTCCA+TGG | 0.457846 | 1.1:-25673242 | None:intergenic |
| TCATCCGGAGGGGTGATGAC+TGG | 0.463619 | 1.1:-25673522 | None:intergenic |
| CTCCGGATGAAGGGCCTACA+AGG | 0.468529 | 1.1:+25673535 | MS.gene65450:CDS |
| CGAGCATAATATTCTCTCGC+TGG | 0.479992 | 1.1:-25673306 | None:intergenic |
| CTGGCGGCCCTTCCTCCGGA+GGG | 0.492619 | 1.1:-25673503 | None:intergenic |
| CGGCCCTTCCTCCGGAGGGG+AGG | 0.493116 | 1.1:-25673499 | None:intergenic |
| CCACCACCCCACCTCCCCTC+CGG | 0.497610 | 1.1:+25673488 | MS.gene65450:CDS |
| CCTCTATGCTAAAATCAGTC+AGG | 0.505084 | 1.1:-25673447 | None:intergenic |
| GGCCTTGTAGGCCCTTCATC+CGG | 0.513242 | 1.1:-25673537 | None:intergenic |
| TTGTAGGCCCTTCATCCGGA+GGG | 0.517268 | 1.1:-25673533 | None:intergenic |
| TCATCACCCCTCCGGATGAA+GGG | 0.523700 | 1.1:+25673526 | MS.gene65450:CDS |
| TTACTCCTCGAGACAGTGAC+TGG | 0.524639 | 1.1:-25673558 | None:intergenic |
| GCCGCCAGTCATCACCCCTC+CGG | 0.525392 | 1.1:+25673518 | MS.gene65450:CDS |
| CCACCTCCCCTCCGGAGGAA+GGG | 0.529795 | 1.1:+25673496 | MS.gene65450:CDS |
| ACTGGCGGCCCTTCCTCCGG+AGG | 0.536576 | 1.1:-25673504 | None:intergenic |
| TCAAACTCGAGTGATGAGTA+AGG | 0.541900 | 1.1:+25673369 | MS.gene65450:CDS |
| CTTGTAGGCCCTTCATCCGG+AGG | 0.566838 | 1.1:-25673534 | None:intergenic |
| CATGGACCCAGAGGCGATAG+GGG | 0.566921 | 1.1:+25673243 | None:intergenic |
| TCCGGAGGGGTGATGACTGG+CGG | 0.577787 | 1.1:-25673519 | None:intergenic |
| GTTCAACCCCTATCGCCTCT+GGG | 0.580458 | 1.1:-25673249 | None:intergenic |
| TGGCGGCCCTTCCTCCGGAG+GGG | 0.581254 | 1.1:-25673502 | None:intergenic |
| CCACCCCACCTCCCCTCCGG+AGG | 0.589127 | 1.1:+25673491 | MS.gene65450:CDS |
| TCCATGGACCCAGAGGCGAT+AGG | 0.601381 | 1.1:+25673241 | None:intergenic |
| TGTAGGCCCTTCATCCGGAG+GGG | 0.625900 | 1.1:-25673532 | None:intergenic |
| CAAGGCCAGTCACTGTCTCG+AGG | 0.640659 | 1.1:+25673553 | MS.gene65450:CDS |
| ACTGGTTTCCATGGACCCAG+AGG | 0.696737 | 1.1:+25673234 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAACTTGTTCACATTTTGA+TGG | + | chr1.1:25673267-25673286 | MS.gene65450:CDS | 30.0% |
| ! | ATTTCGTTGAAGCTTTTGCT+CGG | + | chr1.1:25673401-25673420 | MS.gene65450:CDS | 35.0% |
| !! | CTGACTGATTTTAGCATAGA+GGG | + | chr1.1:25673448-25673467 | MS.gene65450:CDS | 35.0% |
| CCTCTATGCTAAAATCAGTC+AGG | - | chr1.1:25673450-25673469 | None:intergenic | 40.0% | |
| TCAAACTCGAGTGATGAGTA+AGG | + | chr1.1:25673369-25673388 | MS.gene65450:CDS | 40.0% | |
| !! | ACTGATTTTAGCATAGAGGG+AGG | + | chr1.1:25673451-25673470 | MS.gene65450:CDS | 40.0% |
| !! | CCTGACTGATTTTAGCATAG+AGG | + | chr1.1:25673447-25673466 | MS.gene65450:CDS | 40.0% |
| !! | CTGATTTTAGCATAGAGGGA+GGG | + | chr1.1:25673452-25673471 | MS.gene65450:CDS | 40.0% |
| CGAGCATAATATTCTCTCGC+TGG | - | chr1.1:25673309-25673328 | None:intergenic | 45.0% | |
| ! | GCATAGAGGGAGGGTTTTTA+AGG | + | chr1.1:25673461-25673480 | MS.gene65450:CDS | 45.0% |
| TTACTCCTCGAGACAGTGAC+TGG | - | chr1.1:25673561-25673580 | None:intergenic | 50.0% | |
| AGTTCAACCCCTATCGCCTC+TGG | - | chr1.1:25673253-25673272 | None:intergenic | 55.0% | |
| GTTCAACCCCTATCGCCTCT+GGG | - | chr1.1:25673252-25673271 | None:intergenic | 55.0% | |
| TCATCACCCCTCCGGATGAA+GGG | + | chr1.1:25673526-25673545 | MS.gene65450:CDS | 55.0% | |
| TTGTAGGCCCTTCATCCGGA+GGG | - | chr1.1:25673536-25673555 | None:intergenic | 55.0% | |
| !! | GAGACAGTGACTGGCCTTGT+AGG | - | chr1.1:25673552-25673571 | None:intergenic | 55.0% |
| CAAGGCCAGTCACTGTCTCG+AGG | + | chr1.1:25673553-25673572 | MS.gene65450:CDS | 60.0% | |
| CTCCGGATGAAGGGCCTACA+AGG | + | chr1.1:25673535-25673554 | MS.gene65450:CDS | 60.0% | |
| CTTGTAGGCCCTTCATCCGG+AGG | - | chr1.1:25673537-25673556 | None:intergenic | 60.0% | |
| GTCATCACCCCTCCGGATGA+AGG | + | chr1.1:25673525-25673544 | MS.gene65450:CDS | 60.0% | |
| TCATCCGGAGGGGTGATGAC+TGG | - | chr1.1:25673525-25673544 | None:intergenic | 60.0% | |
| TGTAGGCCCTTCATCCGGAG+GGG | - | chr1.1:25673535-25673554 | None:intergenic | 60.0% | |
| ! | GGCCTTGTAGGCCCTTCATC+CGG | - | chr1.1:25673540-25673559 | None:intergenic | 60.0% |
| ATGACTGGCGGCCCTTCCTC+CGG | - | chr1.1:25673510-25673529 | None:intergenic | 65.0% | |
| TCCGGAGGGGTGATGACTGG+CGG | - | chr1.1:25673522-25673541 | None:intergenic | 65.0% | |
| CCACCTCCCCTCCGGAGGAA+GGG | + | chr1.1:25673496-25673515 | MS.gene65450:CDS | 70.0% | |
| CCTTCCTCCGGAGGGGAGGT+GGG | - | chr1.1:25673498-25673517 | None:intergenic | 70.0% | |
| CTTCCTCCGGAGGGGAGGTG+GGG | - | chr1.1:25673497-25673516 | None:intergenic | 70.0% | |
| GCCGCCAGTCATCACCCCTC+CGG | + | chr1.1:25673518-25673537 | MS.gene65450:CDS | 70.0% | |
| ACTGGCGGCCCTTCCTCCGG+AGG | - | chr1.1:25673507-25673526 | None:intergenic | 75.0% | |
| CCACCACCCCACCTCCCCTC+CGG | + | chr1.1:25673488-25673507 | MS.gene65450:CDS | 75.0% | |
| CCCACCTCCCCTCCGGAGGA+AGG | + | chr1.1:25673495-25673514 | MS.gene65450:CDS | 75.0% | |
| CCCTTCCTCCGGAGGGGAGG+TGG | - | chr1.1:25673499-25673518 | None:intergenic | 75.0% | |
| CTGGCGGCCCTTCCTCCGGA+GGG | - | chr1.1:25673506-25673525 | None:intergenic | 75.0% | |
| TGGCGGCCCTTCCTCCGGAG+GGG | - | chr1.1:25673505-25673524 | None:intergenic | 75.0% | |
| ! | AGGGGAGGTGGGGTGGTGGC+TGG | - | chr1.1:25673487-25673506 | None:intergenic | 75.0% |
| ! | CCACCCCACCTCCCCTCCGG+AGG | + | chr1.1:25673491-25673510 | MS.gene65450:CDS | 80.0% |
| ! | CCGGAGGGGAGGTGGGGTGG+TGG | - | chr1.1:25673491-25673510 | None:intergenic | 80.0% |
| ! | CCTCCGGAGGGGAGGTGGGG+TGG | - | chr1.1:25673494-25673513 | None:intergenic | 80.0% |
| ! | CGGCCCTTCCTCCGGAGGGG+AGG | - | chr1.1:25673502-25673521 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 25673244 | 25673588 | 25673244 | ID=MS.gene65450 |
| chr1.1 | mRNA | 25673244 | 25673588 | 25673244 | ID=MS.gene65450.t1;Parent=MS.gene65450 |
| chr1.1 | exon | 25673244 | 25673588 | 25673244 | ID=MS.gene65450.t1.exon1;Parent=MS.gene65450.t1 |
| chr1.1 | CDS | 25673244 | 25673588 | 25673244 | ID=cds.MS.gene65450.t1;Parent=MS.gene65450.t1 |
| Gene Sequence |
| Protein sequence |
