Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65456.t1 | XP_013467071.1 | 85.9 | 198 | 28 | 0 | 1 | 198 | 1 | 198 | 9.60E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65456.t1 | C0HIA3 | 73.2 | 56 | 15 | 0 | 1 | 56 | 1 | 56 | 3.2e-19 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65456.t1 | A0A072VHK1 | 85.9 | 198 | 28 | 0 | 1 | 198 | 1 | 198 | 6.9e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene65456.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65456.t1 | MTR_1g041765 | 87.374 | 198 | 25 | 0 | 1 | 198 | 1 | 198 | 4.25e-109 | 310 |
| MS.gene65456.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 7.97e-35 | 124 |
| MS.gene65456.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 7.97e-35 | 124 |
| MS.gene65456.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 8.66e-34 | 124 |
| MS.gene65456.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.37e-33 | 121 |
| MS.gene65456.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.98e-33 | 121 |
| MS.gene65456.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.99e-33 | 120 |
| MS.gene65456.t1 | MTR_0095s0040 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 1.57e-27 | 104 |
| MS.gene65456.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 4.75e-27 | 104 |
| MS.gene65456.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 4.77e-27 | 104 |
| MS.gene65456.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 1.71e-26 | 104 |
| MS.gene65456.t1 | MTR_2g031870 | 72.131 | 61 | 17 | 0 | 1 | 61 | 1 | 61 | 2.17e-26 | 102 |
| MS.gene65456.t1 | MTR_8g092810 | 71.930 | 57 | 16 | 0 | 1 | 57 | 1 | 57 | 2.07e-23 | 96.7 |
| MS.gene65456.t1 | MTR_2g099610 | 67.857 | 56 | 18 | 0 | 1 | 56 | 1 | 56 | 1.01e-20 | 87.8 |
| MS.gene65456.t1 | MTR_8g018410 | 60.345 | 58 | 23 | 0 | 1 | 58 | 1 | 58 | 1.36e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65456.t1 | AT5G67580 | 63.529 | 85 | 29 | 1 | 1 | 83 | 1 | 85 | 7.27e-29 | 109 |
| MS.gene65456.t1 | AT5G67580 | 63.529 | 85 | 29 | 1 | 1 | 83 | 1 | 85 | 7.27e-29 | 109 |
| MS.gene65456.t1 | AT3G49850 | 68.657 | 67 | 21 | 0 | 1 | 67 | 1 | 67 | 4.14e-27 | 104 |
| MS.gene65456.t1 | AT3G49850 | 68.657 | 67 | 21 | 0 | 1 | 67 | 1 | 67 | 4.14e-27 | 104 |
| MS.gene65456.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 1.24e-25 | 100 |
| MS.gene65456.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 1.24e-25 | 100 |
| MS.gene65456.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 1.24e-25 | 100 |
| MS.gene65456.t1 | AT1G72740 | 63.793 | 58 | 21 | 0 | 1 | 58 | 1 | 58 | 5.55e-20 | 85.5 |
| MS.gene65456.t1 | AT1G72740 | 59.375 | 64 | 26 | 0 | 1 | 64 | 1 | 64 | 5.59e-20 | 85.5 |
| MS.gene65456.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 9.49e-20 | 85.1 |
| MS.gene65456.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 9.75e-20 | 85.1 |
| MS.gene65456.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 9.95e-20 | 85.1 |
| MS.gene65456.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 1.02e-19 | 85.1 |
Find 28 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAAACTAGTGTTGAATTC+TGG | 0.175108 | 1.1:+25748386 | None:intergenic |
| TCATTTCTCTCAGTTGCTTT+TGG | 0.194918 | 1.1:+25748155 | None:intergenic |
| GGCTGAGGAAGCAACTTATA+AGG | 0.388838 | 1.1:-25748000 | MS.gene65456:CDS |
| GCAAACTAGTGTTGAATTCT+GGG | 0.405933 | 1.1:+25748387 | None:intergenic |
| TAAGGGTCTCACACCTCAAC+AGG | 0.416868 | 1.1:-25748075 | MS.gene65456:CDS |
| CAGAGCTGCCTCTTCTTCTC+CGG | 0.417460 | 1.1:+25748463 | None:intergenic |
| GAATATCAATATTCGATCAT+AGG | 0.430998 | 1.1:-25748226 | MS.gene65456:CDS |
| ATGTAGACCTCAAGGACAAA+TGG | 0.452440 | 1.1:-25748352 | MS.gene65456:CDS |
| GAAGAGGCAGCTCTGAAAGC+GGG | 0.486977 | 1.1:-25748455 | MS.gene65456:CDS |
| GGCAGAAGCTGCCATGACTA+AGG | 0.496803 | 1.1:-25748021 | MS.gene65456:CDS |
| GGAGTAGTCAAACACGGAGC+TGG | 0.501232 | 1.1:-25748434 | MS.gene65456:CDS |
| AGTTCCTCCATTTGTCCTTG+AGG | 0.505844 | 1.1:+25748345 | None:intergenic |
| TCAAACACGGAGCTGGAAGA+TGG | 0.524401 | 1.1:-25748427 | MS.gene65456:CDS |
| CGACGACAACATTTAGATCA+AGG | 0.524618 | 1.1:-25748316 | MS.gene65456:CDS |
| AATAATAATCAATATTCAAG+TGG | 0.553519 | 1.1:-25748248 | MS.gene65456:CDS |
| AAAATGGACCGGAGAAGAAG+AGG | 0.564638 | 1.1:-25748471 | MS.gene65456:CDS |
| AGCTGCAAAAGCAATGGAGG+AGG | 0.570145 | 1.1:-25748042 | MS.gene65456:CDS |
| AGCTGCCATGACTAAGGCTG+AGG | 0.573794 | 1.1:-25748015 | MS.gene65456:CDS |
| GGAAGCAACTTATAAGGCAG+AGG | 0.583297 | 1.1:-25747994 | MS.gene65456:CDS |
| TGCTTCCTCAGCCTTAGTCA+TGG | 0.584066 | 1.1:+25748010 | None:intergenic |
| AGAAGAAGCTGCAAAAGCAA+TGG | 0.585302 | 1.1:-25748048 | MS.gene65456:CDS |
| TGCAAAAGCAGAAATGAAGA+AGG | 0.586897 | 1.1:-25747937 | MS.gene65456:CDS |
| GCTTCTTCTATCGCCTGTTG+AGG | 0.590627 | 1.1:+25748062 | None:intergenic |
| AGAAGCTGCAAAAGCAATGG+AGG | 0.603836 | 1.1:-25748045 | MS.gene65456:CDS |
| TCGTTCAAATGTAGACCTCA+AGG | 0.605301 | 1.1:-25748360 | MS.gene65456:CDS |
| AGAAGAGGCAGCTCTGAAAG+CGG | 0.618500 | 1.1:-25748456 | MS.gene65456:CDS |
| TAGACCTCAAGGACAAATGG+AGG | 0.657376 | 1.1:-25748349 | MS.gene65456:CDS |
| AAAGCGGGAGTAGTCAAACA+CGG | 0.721145 | 1.1:-25748440 | MS.gene65456:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATCAATATTCAAG+TGG | - | chr1.1:25748154-25748173 | MS.gene65456:CDS | 15.0% |
| !!! | AGAGATTTTTAAAATGATTA+AGG | - | chr1.1:25748309-25748328 | MS.gene65456:CDS | 15.0% |
| !!! | GAGATTTTTAAAATGATTAA+GGG | - | chr1.1:25748310-25748329 | MS.gene65456:CDS | 15.0% |
| !!! | TGATTATTATTTTCAACTTT+AGG | + | chr1.1:25748145-25748164 | None:intergenic | 15.0% |
| !!! | TAATTTCTTCGTATTTTGAA+AGG | + | chr1.1:25748285-25748304 | None:intergenic | 20.0% |
| !!! | TTATTATTTTCAACTTTAGG+TGG | + | chr1.1:25748142-25748161 | None:intergenic | 20.0% |
| ! | GAATATCAATATTCGATCAT+AGG | - | chr1.1:25748176-25748195 | MS.gene65456:CDS | 25.0% |
| ! | TTATTTTCAACTTTAGGTGG+AGG | + | chr1.1:25748139-25748158 | None:intergenic | 30.0% |
| AACTTCAACAAAAATACGCG+AGG | - | chr1.1:25748207-25748226 | MS.gene65456:CDS | 35.0% | |
| TGCAAAAGCAGAAATGAAGA+AGG | - | chr1.1:25748465-25748484 | MS.gene65456:CDS | 35.0% | |
| ! | GCAAACTAGTGTTGAATTCT+GGG | + | chr1.1:25748018-25748037 | None:intergenic | 35.0% |
| !! | GCGTATTTTTGTTGAAGTTG+AGG | + | chr1.1:25748206-25748225 | None:intergenic | 35.0% |
| !! | TCATTTCTCTCAGTTGCTTT+TGG | + | chr1.1:25748250-25748269 | None:intergenic | 35.0% |
| AGAAGAAGCTGCAAAAGCAA+TGG | - | chr1.1:25748354-25748373 | MS.gene65456:CDS | 40.0% | |
| ATGTAGACCTCAAGGACAAA+TGG | - | chr1.1:25748050-25748069 | MS.gene65456:CDS | 40.0% | |
| CGACGACAACATTTAGATCA+AGG | - | chr1.1:25748086-25748105 | MS.gene65456:CDS | 40.0% | |
| TCGTTCAAATGTAGACCTCA+AGG | - | chr1.1:25748042-25748061 | MS.gene65456:CDS | 40.0% | |
| ! | CGCAAACTAGTGTTGAATTC+TGG | + | chr1.1:25748019-25748038 | None:intergenic | 40.0% |
| AAAATGGACCGGAGAAGAAG+AGG | - | chr1.1:25747931-25747950 | MS.gene65456:CDS | 45.0% | |
| AAAGCGGGAGTAGTCAAACA+CGG | - | chr1.1:25747962-25747981 | MS.gene65456:CDS | 45.0% | |
| AGAAGCTGCAAAAGCAATGG+AGG | - | chr1.1:25748357-25748376 | MS.gene65456:CDS | 45.0% | |
| AGTTCCTCCATTTGTCCTTG+AGG | + | chr1.1:25748060-25748079 | None:intergenic | 45.0% | |
| GCTCCTAAGCAAAAATGGAC+CGG | - | chr1.1:25747920-25747939 | MS.gene65456:CDS | 45.0% | |
| GGAAGCAACTTATAAGGCAG+AGG | - | chr1.1:25748408-25748427 | MS.gene65456:CDS | 45.0% | |
| GGCTGAGGAAGCAACTTATA+AGG | - | chr1.1:25748402-25748421 | MS.gene65456:CDS | 45.0% | |
| TAGACCTCAAGGACAAATGG+AGG | - | chr1.1:25748053-25748072 | MS.gene65456:CDS | 45.0% | |
| TGGGTGCTCCTAAGCAAAAA+TGG | - | chr1.1:25747915-25747934 | MS.gene65456:CDS | 45.0% | |
| !! | TCTCCGGTCCATTTTTGCTT+AGG | + | chr1.1:25747926-25747945 | None:intergenic | 45.0% |
| AGCTGCAAAAGCAATGGAGG+AGG | - | chr1.1:25748360-25748379 | MS.gene65456:CDS | 50.0% | |
| GCTTCTTCTATCGCCTGTTG+AGG | + | chr1.1:25748343-25748362 | None:intergenic | 50.0% | |
| TAAGGGTCTCACACCTCAAC+AGG | - | chr1.1:25748327-25748346 | MS.gene65456:CDS | 50.0% | |
| TCAAACACGGAGCTGGAAGA+TGG | - | chr1.1:25747975-25747994 | MS.gene65456:CDS | 50.0% | |
| TGCTTCCTCAGCCTTAGTCA+TGG | + | chr1.1:25748395-25748414 | None:intergenic | 50.0% | |
| !! | AGAAGAGGCAGCTCTGAAAG+CGG | - | chr1.1:25747946-25747965 | MS.gene65456:CDS | 50.0% |
| AGCTGCCATGACTAAGGCTG+AGG | - | chr1.1:25748387-25748406 | MS.gene65456:CDS | 55.0% | |
| CAGAGCTGCCTCTTCTTCTC+CGG | + | chr1.1:25747942-25747961 | None:intergenic | 55.0% | |
| GGAGTAGTCAAACACGGAGC+TGG | - | chr1.1:25747968-25747987 | MS.gene65456:CDS | 55.0% | |
| GGCAGAAGCTGCCATGACTA+AGG | - | chr1.1:25748381-25748400 | MS.gene65456:CDS | 55.0% | |
| !! | GAAGAGGCAGCTCTGAAAGC+GGG | - | chr1.1:25747947-25747966 | MS.gene65456:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 25747914 | 25748510 | 25747914 | ID=MS.gene65456 |
| chr1.1 | mRNA | 25747914 | 25748510 | 25747914 | ID=MS.gene65456.t1;Parent=MS.gene65456 |
| chr1.1 | exon | 25747914 | 25748510 | 25747914 | ID=MS.gene65456.t1.exon1;Parent=MS.gene65456.t1 |
| chr1.1 | CDS | 25747914 | 25748510 | 25747914 | ID=cds.MS.gene65456.t1;Parent=MS.gene65456.t1 |
| Gene Sequence |
| Protein sequence |
