AlfalfaGEDB | MS.gene65492

M. sativa cultivar XinJiangDaYe / MS.gene65492

KEGG: K09422
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65492.t1	ABR28344.1	79.1	177	30	3	1	176	85	255	5.30E-62	247.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65492.t1	Q9SJX8	34.1	185	93	6	1	176	85	249	1.0e-13	78.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65492.t1	G7I4Q3	79.1	177	30	3	1	176	85	255	3.9e-62	247.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene65492.t1	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65492.t1	MTR_1g043050	78.409	176	32	1	1	176	85	254	5.46e-97	281
MS.gene65492.t1	MTR_1g043080	81.921	177	25	3	1	176	85	255	7.55e-90	263
MS.gene65492.t1	MTR_1g076150	52.809	178	72	5	4	176	36	206	4.94e-46	150
MS.gene65492.t1	MTR_1g076150	51.934	181	75	5	1	176	85	258	3.22e-45	149
MS.gene65492.t1	MTR_7g096930	34.826	201	99	6	1	176	85	278	5.51e-20	84.7
MS.gene65492.t1	MTR_7g115650	71.875	32	9	0	1	32	85	116	2.90e-11	60.1
MS.gene65492.t1	MTR_5g014990	48.571	70	28	3	1	68	86	149	5.05e-11	60.5
MS.gene65492.t1	MTR_2g089620	72.727	33	9	0	1	33	85	117	5.59e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65492.t1	AT3G23250	38.308	201	92	7	1	173	28	224	5.64e-25	96.7
MS.gene65492.t1	AT3G23250	38.308	201	92	7	1	173	85	281	3.14e-24	96.3
MS.gene65492.t1	AT1G06180	37.433	187	81	9	1	176	85	246	3.39e-18	79.3
MS.gene65492.t1	AT2G31180	35.135	185	91	7	1	176	85	249	7.25e-18	78.6
MS.gene65492.t1	AT1G16490	93.103	29	2	0	4	32	21	49	4.75e-13	64.7
MS.gene65492.t1	AT4G28110	78.788	33	7	0	1	33	1	33	1.16e-12	63.5
MS.gene65492.t1	AT3G61250	45.205	73	34	1	1	67	85	157	2.44e-12	63.9
MS.gene65492.t1	AT1G16490	89.655	29	3	0	4	32	90	118	8.94e-12	62.4
MS.gene65492.t1	AT4G05100	75.758	33	8	0	1	33	86	118	1.89e-11	61.6
MS.gene65492.t1	AT1G79180	78.125	32	7	0	4	35	90	121	3.00e-11	60.8

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Find 19 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCCAATTCCAACTCAA+TGG	0.490366	1.3:-25754002	MS.gene65492:CDS
AATTCATTTGAAACTGTCAT+AGG	0.356516	1.3:+25753800	None:intergenic
AGCTTCCATTGAGTTGGAAT+TGG	0.389117	1.3:+25753997	None:intergenic
ATGCCATCGTCATTATCAAA+AGG	0.461830	1.3:+25753728	None:intergenic
ATTGTCACAAAAGAGTCTTC+AGG	0.488694	1.3:+25753896	None:intergenic
ATTTCATTGTCTGTTCTTCC+AGG	0.291634	1.3:+25754139	None:intergenic
CAAAAGCTTTCATCAATTTC+AGG	0.250594	1.3:+25753872	None:intergenic
CATAGGATTTGATGGCATGG+TGG	0.687440	1.3:+25753817	None:intergenic
CATGAAAATGTTTAGAACTC+TGG	0.425811	1.3:+25753656	None:intergenic
CGTCATTATCAAAAGGGTTT+TGG	0.211007	1.3:+25753735	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAAAGCTTTCATCAATTTC+AGG	+	chr1.3:25754155-25754174	None:intergenic	30.0%
TATAACGTGTTCATCAAATC+AGG	-	chr1.3:25754329-25754348	MS.gene65492:intron	30.0%
AAATGCCAATTCCAACTCAA+TGG	-	chr1.3:25754022-25754041	MS.gene65492:CDS	35.0%
ATGCCATCGTCATTATCAAA+AGG	+	chr1.3:25754299-25754318	None:intergenic	35.0%
ATGTTGTAATTGCATGTGGT+TGG	+	chr1.3:25753713-25753732	None:intergenic	35.0%
ATTGTCACAAAAGAGTCTTC+AGG	+	chr1.3:25754131-25754150	None:intergenic	35.0%
ATTTCATTGTCTGTTCTTCC+AGG	+	chr1.3:25753888-25753907	None:intergenic	35.0%
GAAACTGTCATAGGATTTGA+TGG	+	chr1.3:25754218-25754237	None:intergenic	35.0%
TGCCATCGTCATTATCAAAA+GGG	+	chr1.3:25754298-25754317	None:intergenic	35.0%
TTGTCACAAAAGAGTCTTCA+GGG	+	chr1.3:25754130-25754149	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	25753667	25754379	25753667	ID=MS.gene65492
chr1.3	mRNA	25753667	25754379	25753667	ID=MS.gene65492.t1;Parent=MS.gene65492
chr1.3	exon	25754369	25754379	25754369	ID=MS.gene65492.t1.exon1;Parent=MS.gene65492.t1
chr1.3	CDS	25754369	25754379	25754369	ID=cds.MS.gene65492.t1;Parent=MS.gene65492.t1
chr1.3	exon	25753667	25754186	25753667	ID=MS.gene65492.t1.exon2;Parent=MS.gene65492.t1
chr1.3	CDS	25753667	25754186	25753667	ID=cds.MS.gene65492.t1;Parent=MS.gene65492.t1

Gene Sequence

>MS.gene65492

ATGAATAAAAGGTAAGCCGACAACTAAGCATTAATTTGTAATACCAACCACATGCAATTACAACATATTTATATATATTATTGACATACATAGCACTATGCATGAATACAGTCAAAATTTGTTGTTTTCTCAATAACTTTATCCAAGAAGCAAAATTCGAATCTCATACATTAATTCTGATTTTTGTTTTCAGATGGTCAGCAATAGCAGCAAAATTACCTGGAAGAACAGACAATGAAATAAAAAATGTATGGCACACTCACTTGAAGAAAAGACTACTGAATACCAAAAATAACCAACCTCATTCAAATACCAAAAAAAGAGTGTCAAAACAAAAAATCAAAAGATATGATTCAAATGCCAATTCCAACTCAATGGAAGCTACATCATCAAATTGCACTTTTTCTAGTAATTTTTCATCACAAGGAAGAAATGTTGACAACATCATCAATTGTGAAGACCCTGAAGACTCTTTTGTGACAATGCCTGAAATTGATGAAAGCTTTTGGTCAGAGAAAATAAATGATGACGAAATTAGCTCCACCATGCCATCAAATCCTATGACAGTTTCAAATGAATTACCAGATCAACAATATATTTTTAATAATTCAGTAGAAAATTTCCAAAACCCTTTTGATAATGACGATGGCATGGATTTTTGGTATAACGTGTTCATCAAATCAGGAGAATCAACAGAATTGCCAGAGTTCTAA

Protein sequence

>MS.gene65492.t1

MNKRWSAIAAKLPGRTDNEIKNVWHTHLKKRLLNTKNNQPHSNTKKRVSKQKIKRYDSNANSNSMEATSSNCTFSSNFSSQGRNVDNIINCEDPEDSFVTMPEIDESFWSEKINDDEISSTMPSNPMTVSNELPDQQYIFNNSVENFQNPFDNDDGMDFWYNVFIKSGESTELPEF