AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65624

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65624.t1 MTR_8g063870 66.667 333 44 6 1 327 1 272 3.29e-146 413
MS.gene65624.t1 MTR_8g063600 56.686 344 77 6 1 327 1 289 8.42e-115 334
MS.gene65624.t1 MTR_1g111830 51.412 177 61 3 10 169 23 191 4.07e-47 159
MS.gene65624.t1 MTR_4g111975 38.163 283 109 9 11 245 8 272 2.29e-46 159
MS.gene65624.t1 MTR_5g069710 42.347 196 96 5 11 192 29 221 1.36e-43 152
MS.gene65624.t1 MTR_3g462790 45.304 181 78 4 6 169 38 214 4.65e-43 151
MS.gene65624.t1 MTR_5g081860 46.746 169 81 2 10 169 26 194 1.04e-41 146
MS.gene65624.t1 MTR_7g067080 44.693 179 81 4 10 172 34 210 1.54e-40 144
MS.gene65624.t1 MTR_1g033600 51.471 136 43 4 1 114 3 137 1.49e-32 118
MS.gene65624.t1 MTR_5g075760 43.558 163 85 6 7 164 9 169 1.59e-30 115
MS.gene65624.t1 MTR_1g033620 40.654 214 59 6 115 321 20 172 5.56e-30 114
MS.gene65624.t1 MTR_5g075790 41.975 162 88 6 7 164 18 177 6.74e-29 111
MS.gene65624.t1 MTR_5g088010 44.737 114 50 3 99 211 59 160 1.33e-23 97.8
MS.gene65624.t1 MTR_5g088060 33.493 209 110 6 5 211 191 372 6.20e-22 96.3
MS.gene65624.t1 MTR_5g488160 33.493 209 110 6 5 211 191 372 6.20e-22 96.3
MS.gene65624.t1 MTR_5g027570 40.000 120 72 0 101 220 93 212 1.20e-21 92.0
MS.gene65624.t1 MTR_1g022290 41.818 110 46 2 6 115 2 93 2.23e-20 84.7
MS.gene65624.t1 MTR_4g100630 44.915 118 53 4 74 190 78 184 1.37e-19 88.2
MS.gene65624.t1 MTR_2g100930 44.554 101 53 2 67 167 77 174 1.89e-19 88.2
MS.gene65624.t1 MTR_6g092540 52.809 89 33 2 102 183 99 185 2.52e-19 86.7
MS.gene65624.t1 MTR_3g116720 53.846 65 30 0 11 75 16 80 6.23e-19 80.5
MS.gene65624.t1 MTR_8g101650 59.091 66 24 1 102 167 107 169 2.07e-18 84.0
MS.gene65624.t1 MTR_3g111880 56.061 66 29 0 8 73 4 69 3.06e-18 79.0
MS.gene65624.t1 MTR_5g020170 59.091 66 27 0 8 73 16 81 1.44e-17 77.0
MS.gene65624.t1 MTR_4g015130 59.649 57 21 1 110 166 3 57 1.46e-17 76.6
MS.gene65624.t1 MTR_0036s0260 54.930 71 30 1 96 166 24 92 1.56e-17 80.1
MS.gene65624.t1 MTR_2g090305 54.930 71 30 1 96 166 24 92 6.73e-17 77.8
MS.gene65624.t1 MTR_4g107230 75.556 45 11 0 102 146 86 130 1.20e-16 79.3
MS.gene65624.t1 MTR_1g083180 47.170 106 48 5 63 163 22 124 4.02e-16 75.9
MS.gene65624.t1 MTR_5g037080 50.000 84 40 1 102 185 104 185 4.32e-16 78.2
MS.gene65624.t1 MTR_6g004250 52.174 69 33 0 5 73 4 72 6.93e-16 72.0
MS.gene65624.t1 MTR_3g104370 51.389 72 32 2 102 171 93 163 1.74e-15 75.1
MS.gene65624.t1 MTR_5g027550 58.929 56 21 1 91 146 70 123 3.26e-15 74.3
MS.gene65624.t1 MTR_7g089210 51.429 70 34 0 14 83 15 84 6.28e-15 69.7
MS.gene65624.t1 MTR_8g077360 44.286 70 38 1 11 80 5 73 4.00e-14 67.0
MS.gene65624.t1 MTR_1g048660 54.386 57 26 0 102 158 14 70 5.15e-14 67.4
MS.gene65624.t1 MTR_8g077380 44.928 69 37 1 11 79 5 72 5.16e-14 66.6
MS.gene65624.t1 MTR_8g077390 44.928 69 37 1 11 79 5 72 5.16e-14 66.6
MS.gene65624.t1 MTR_3g013440 49.275 69 34 1 6 73 5 73 9.09e-13 63.9
MS.gene65624.t1 MTR_8g073050 37.500 96 47 1 102 197 105 187 1.07e-12 67.0
MS.gene65624.t1 MTR_5g020540 50.820 61 29 1 11 71 4 63 1.28e-12 62.4
MS.gene65624.t1 MTR_3g070110 36.364 143 77 4 50 179 38 179 1.67e-12 66.2
MS.gene65624.t1 MTR_3g111920 48.438 64 31 2 11 73 3 65 3.25e-12 62.0
MS.gene65624.t1 MTR_6g043490 34.043 94 51 1 102 195 109 191 6.54e-12 64.7
MS.gene65624.t1 MTR_6g043480 34.043 94 51 1 102 195 109 191 3.73e-11 62.4
MS.gene65624.t1 MTR_8g077420 44.262 61 33 1 11 71 5 64 3.79e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65624.t1 AT5G08520 41.176 340 116 11 11 324 12 293 6.12e-63 202
MS.gene65624.t1 AT5G23650 36.232 345 155 11 11 314 13 333 1.26e-45 158
MS.gene65624.t1 AT5G04760 48.503 167 78 3 8 169 3 166 9.32e-43 147
MS.gene65624.t1 AT2G38090 43.005 193 84 5 6 174 24 214 2.70e-42 148
MS.gene65624.t1 AT1G49010 42.157 204 79 4 11 177 8 209 2.93e-42 149
MS.gene65624.t1 AT5G58900 41.808 177 83 3 11 169 34 208 3.88e-40 142
MS.gene65624.t1 AT3G11280 44.512 164 78 4 11 166 33 191 7.17e-39 138
MS.gene65624.t1 AT3G11280 44.512 164 78 4 11 166 33 191 7.17e-39 138
MS.gene65624.t1 AT5G05790 41.111 180 81 3 7 169 27 198 1.47e-37 135
MS.gene65624.t1 AT5G05790 41.111 180 81 3 7 169 27 198 1.47e-37 135
MS.gene65624.t1 AT5G01200 40.000 190 85 5 7 169 26 213 2.97e-34 126
MS.gene65624.t1 AT4G09450 40.506 158 87 3 10 167 6 156 1.40e-25 102
MS.gene65624.t1 AT3G10580 40.506 158 86 4 11 167 8 158 5.23e-24 99.0
MS.gene65624.t1 AT3G10580 40.506 158 86 4 11 167 8 158 7.85e-24 99.4
MS.gene65624.t1 AT1G75250 54.286 70 32 0 11 80 12 81 2.69e-20 84.7
MS.gene65624.t1 AT1G75250 54.286 70 32 0 11 80 12 81 1.10e-19 84.0
MS.gene65624.t1 AT4G39250 53.968 63 29 0 11 73 14 76 5.06e-19 81.3
MS.gene65624.t1 AT5G47390 48.958 96 34 3 72 167 81 161 9.91e-19 86.3
MS.gene65624.t1 AT3G10590 34.444 180 96 7 11 173 7 181 2.09e-18 82.4
MS.gene65624.t1 AT3G16350 55.844 77 25 2 70 146 116 183 2.84e-17 82.4
MS.gene65624.t1 AT5G56840 61.667 60 23 0 102 161 93 152 4.35e-17 79.7
MS.gene65624.t1 AT1G19510 47.887 71 37 0 14 84 15 85 7.05e-17 75.5
MS.gene65624.t1 AT5G61620 56.061 66 27 1 102 167 110 173 8.32e-17 80.1
MS.gene65624.t1 AT2G21650 48.611 72 37 0 11 82 14 85 1.00e-16 75.1
MS.gene65624.t1 AT1G70000 75.556 45 11 0 102 146 100 144 2.41e-16 78.2
MS.gene65624.t1 AT1G70000 75.556 45 11 0 102 146 100 144 2.41e-16 78.2
MS.gene65624.t1 AT1G74840 68.889 45 14 0 102 146 98 142 4.57e-16 76.6
MS.gene65624.t1 AT1G74840 68.889 45 14 0 102 146 98 142 5.47e-16 77.0
MS.gene65624.t1 AT1G19000 66.667 45 15 0 102 146 104 148 1.41e-14 73.2
MS.gene65624.t1 AT1G19000 66.667 45 15 0 102 146 104 148 1.41e-14 73.2
MS.gene65624.t1 AT2G18328 52.542 59 28 0 15 73 15 73 3.47e-14 67.4
MS.gene65624.t1 AT3G10595 24.859 177 118 4 11 187 6 167 7.18e-12 63.9

Find 97 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACATCACTATGAGATTT+TGG 0.187100 8.2:-38009843 MS.gene65624:CDS
TGGATGATCATCTAAATTTC+TGG 0.206174 8.2:+38008646 None:intergenic
TATGTTGCTGCCCCGCCTTC+TGG 0.226151 8.2:-38008671 MS.gene65624:CDS
GATGAGCAGGTTATTTCTTA+TGG 0.229350 8.2:-38009174 MS.gene65624:intron
TCGCTTGCTTGACTGGTAAT+TGG 0.244402 8.2:+38008969 None:intergenic
TAGAGGATTATTTAAAGTTC+TGG 0.291908 8.2:+38008896 None:intergenic
GTATCTGCTTTCGGCGCTGC+AGG 0.317922 8.2:-38008557 MS.gene65624:CDS
TCTGTTGGAACTAGAAATTT+AGG 0.321437 8.2:-38008840 MS.gene65624:intron
AGGCTTCCGGTTCTTATCAT+TGG 0.328156 8.2:-38009706 MS.gene65624:CDS
ATGGTATTGGACCAGTTTCT+TGG 0.328363 8.2:-38008505 MS.gene65624:CDS
TGAAAGAGGAGCTGTGAAAA+AGG 0.334187 8.2:-38009735 MS.gene65624:CDS
GATCATCCAGCCCCACTTGC+TGG 0.353540 8.2:-38008632 MS.gene65624:CDS
ATGAGCAGGTTATTTCTTAT+GGG 0.354456 8.2:-38009173 MS.gene65624:intron
TCTTTCTTTCTTCTCTTTCG+TGG 0.354521 8.2:+38009037 None:intergenic
GGCATATTTGTCCAAGAAAC+TGG 0.357689 8.2:+38008494 None:intergenic
GATATCCACAACATTGAATC+AGG 0.363868 8.2:-38009815 MS.gene65624:CDS
CTTATGGGCGTGGAGAAGTA+TGG 0.382631 8.2:-38009158 MS.gene65624:CDS
GCTCCCGGACACATGGCTTA+TGG 0.384505 8.2:-38008524 MS.gene65624:CDS
GGACACATGGCTTATGGTAT+TGG 0.393934 8.2:-38008518 MS.gene65624:CDS
TGCAGAATCGCTTGCTTGAC+TGG 0.396522 8.2:+38008962 None:intergenic
AGACGGGTTGTATCTGCTTT+CGG 0.398815 8.2:-38008566 MS.gene65624:CDS
GCAGGGTTTCCTTCTGCTCC+CGG 0.401027 8.2:-38008539 MS.gene65624:CDS
CCAGCCCCACTTGCTGGACT+CGG 0.416423 8.2:-38008626 MS.gene65624:CDS
ATAGGCGACATCGTGTATTC+TGG 0.417387 8.2:+38009007 None:intergenic
AACAGCATCCTCGGGATGAT+TGG 0.418808 8.2:+38009919 None:intergenic
TTCTGTCCAATGATAAGAAC+CGG 0.425400 8.2:+38009700 None:intergenic
AGAAGTATGGAAAGGGTAAA+TGG 0.436340 8.2:-38009145 MS.gene65624:CDS
CCTTGGATCGGGCAACCTAT+AGG 0.440600 8.2:-38008590 MS.gene65624:CDS
GCCAGGGGTACCAATGAACT+TGG 0.443338 8.2:-38008474 MS.gene65624:CDS
GTTTATGATGTGCCTTGGAT+CGG 0.445975 8.2:-38008602 MS.gene65624:CDS
CCGAGTCCAGCAAGTGGGGC+TGG 0.451356 8.2:+38008626 None:intergenic
GATACAACCCGTCTTCCTAT+AGG 0.454270 8.2:+38008575 None:intergenic
CCATCGATCAACAGCATCCT+CGG 0.458092 8.2:+38009910 None:intergenic
TCCTTCTGCTCCCGGACACA+TGG 0.459304 8.2:-38008531 MS.gene65624:CDS
AAAATTGCGGCTGATGTGCC+CGG 0.461117 8.2:-38009884 MS.gene65624:CDS
TTTATGATGTGCCTTGGATC+GGG 0.462141 8.2:-38008601 MS.gene65624:CDS
AATTTCTGGTTCCAGAAGGC+GGG 0.463924 8.2:+38008660 None:intergenic
GTCATAGGACCCAAGTTCAT+TGG 0.473573 8.2:+38008464 None:intergenic
CCCAAGTTCATTGGTACCCC+TGG 0.474063 8.2:+38008473 None:intergenic
TCGGAGTTTATGATGTGCCT+TGG 0.477536 8.2:-38008607 MS.gene65624:CDS
TATCTGCTTTCGGCGCTGCA+GGG 0.484566 8.2:-38008556 MS.gene65624:CDS
CCAGGAATTCTGACTGCTCC+CGG 0.491706 8.2:-38008728 MS.gene65624:intron
CAGGTTATTTCTTATGGGCG+TGG 0.501373 8.2:-38009168 MS.gene65624:CDS
TAAATTGTTCGAAAATGCAT+TGG 0.509004 8.2:-38009942 MS.gene65624:CDS
ACAAAGCGTGCTGGTGAAAG+AGG 0.509612 8.2:-38009749 MS.gene65624:CDS
AATCAAAATAATGGATGAAG+TGG 0.514101 8.2:-38009999 None:intergenic
AATACCATAAGCCATGTGTC+CGG 0.520964 8.2:+38008520 None:intergenic
TATGCTGCTTTGCCATCTGT+TGG 0.524271 8.2:-38008855 MS.gene65624:CDS
CGAGGATGCTGTTGATCGAT+GGG 0.525087 8.2:-38009909 MS.gene65624:CDS
CCGAGGATGCTGTTGATCGA+TGG 0.525902 8.2:-38009910 MS.gene65624:CDS
CGAAAGAGAAGAAAGAAAGA+AGG 0.527182 8.2:-38009034 MS.gene65624:CDS
GGCGTGGAGAAGTATGGAAA+GGG 0.531559 8.2:-38009152 MS.gene65624:CDS
TGGCAAAGCAGCATACATTC+CGG 0.533982 8.2:+38008863 None:intergenic
AAAGTTCTGGTACCAACAGA+TGG 0.539373 8.2:+38008909 None:intergenic
GCCATGTGTCCGGGAGCAGA+AGG 0.540860 8.2:+38008530 None:intergenic
GTTTCTTGGACAAATATGCC+AGG 0.542042 8.2:-38008491 MS.gene65624:CDS
GCTTGAGGAGCATAAACTCC+GGG 0.545991 8.2:+38008710 None:intergenic
CATCGATCAACAGCATCCTC+GGG 0.548607 8.2:+38009911 None:intergenic
CGGGCAACCTATAGGAAGAC+GGG 0.550451 8.2:-38008582 MS.gene65624:CDS
CATGCTCAGAAATACTTCAA+AGG 0.551854 8.2:-38009068 MS.gene65624:CDS
ATACCATAAGCCATGTGTCC+GGG 0.552377 8.2:+38008521 None:intergenic
CAAAACCTGATTCAATGTTG+TGG 0.563820 8.2:+38009810 None:intergenic
CCTATAGGTTGCCCGATCCA+AGG 0.564457 8.2:+38008590 None:intergenic
AATCCTCTAGCTCCACCTGC+CGG 0.568098 8.2:-38008882 MS.gene65624:CDS
AAATTTCTGGTTCCAGAAGG+CGG 0.568201 8.2:+38008659 None:intergenic
ATTTCAGTAGGTGGAGCTTG+AGG 0.570653 8.2:+38008695 None:intergenic
AAGAGCTCAACAAAGCGTGC+TGG 0.576719 8.2:-38009758 MS.gene65624:CDS
ATTCCGGCAGGTGGAGCTAG+AGG 0.580304 8.2:+38008879 None:intergenic
TGGTCCTTGAGGTTTGACAT+AGG 0.583815 8.2:+38008989 None:intergenic
AAAGAATCATAATCTGGCAG+AGG 0.587497 8.2:+38009785 None:intergenic
AAAGCAGCATACATTCCGGC+AGG 0.587858 8.2:+38008867 None:intergenic
AAATTTCTAGTTCCAACAGA+TGG 0.588745 8.2:+38008843 None:intergenic
ATTTCTGGTTCCAGAAGGCG+GGG 0.590827 8.2:+38008661 None:intergenic
TTTCTTGGACAAATATGCCA+GGG 0.593394 8.2:-38008490 MS.gene65624:CDS
GCAGCATACATTCCGGCAGG+TGG 0.596107 8.2:+38008870 None:intergenic
GTACCAACAGATGGAGCTTG+AGG 0.598203 8.2:+38008918 None:intergenic
AAATTGCGGCTGATGTGCCC+GGG 0.599032 8.2:-38009883 MS.gene65624:CDS
GCAACATACATTTCAGTAGG+TGG 0.602800 8.2:+38008686 None:intergenic
TCTAAATTTCTGGTTCCAGA+AGG 0.603646 8.2:+38008656 None:intergenic
GGGCGTGGAGAAGTATGGAA+AGG 0.603697 8.2:-38009153 MS.gene65624:CDS
TCGGGCAACCTATAGGAAGA+CGG 0.605588 8.2:-38008583 MS.gene65624:CDS
ATCAAAATAATGGATGAAGT+GGG 0.606623 8.2:-38009998 None:intergenic
ACTCCTCAAGCTCCATCTGT+TGG 0.611272 8.2:-38008921 MS.gene65624:CDS
AAACTCCGAGTCCAGCAAGT+GGG 0.613833 8.2:+38008621 None:intergenic
GTCGCCTATGTCAAACCTCA+AGG 0.619811 8.2:-38008993 MS.gene65624:CDS
TGACTGGTAATTGGTCCTTG+AGG 0.619996 8.2:+38008978 None:intergenic
GCAGCAACATACATTTCAGT+AGG 0.620747 8.2:+38008683 None:intergenic
AGCTTGAGGAGCATAAACTC+CGG 0.627574 8.2:+38008709 None:intergenic
AACTCCGAGTCCAGCAAGTG+GGG 0.637926 8.2:+38008622 None:intergenic
TGGAGCTTGAGGAGTTTGTG+TGG 0.647801 8.2:+38008929 None:intergenic
CCAGGGGTACCAATGAACTT+GGG 0.650736 8.2:-38008473 MS.gene65624:CDS
TGCATTGGCCAATCATCCCG+AGG 0.663855 8.2:-38009927 MS.gene65624:CDS
TGGGTAGTAGAAGCTATGAG+TGG 0.665232 8.2:-38009979 MS.gene65624:CDS
TTCTTGGACAAATATGCCAG+GGG 0.665492 8.2:-38008489 MS.gene65624:CDS
TAAACTCCGAGTCCAGCAAG+TGG 0.668326 8.2:+38008620 None:intergenic
TGATCGATGGGAGAAAATTG+CGG 0.690864 8.2:-38009897 MS.gene65624:CDS
ATTGGACAGAAGATGAACAC+AGG 0.692851 8.2:-38009688 MS.gene65624:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAACAATACTAATAATA+TGG + chr8.2:38009213-38009232 None:intergenic 10.0%
!! TAATGATATGATACTAATTA+AGG - chr8.2:38008841-38008860 MS.gene65624:CDS 15.0%
!! AAAAATATCAGAGAATTATG+TGG - chr8.2:38009302-38009321 MS.gene65624:intron 20.0%
!! ATACAATAATTCCTTATACA+TGG + chr8.2:38009072-38009091 None:intergenic 20.0%
!! GTTATTATTTACAACTTACA+TGG - chr8.2:38008803-38008822 MS.gene65624:intron 20.0%
!! TTTGAAAAAGAATCATAATC+TGG + chr8.2:38008646-38008665 None:intergenic 20.0%
!!! AATAGCTTAATTATTTCTAG+AGG - chr8.2:38009120-38009139 MS.gene65624:CDS 20.0%
!!! GTTTCTTTAATTAGTTTCAA+TGG - chr8.2:38008968-38008987 MS.gene65624:CDS 20.0%
! AAAATGCACACATATTGATA+CGG + chr8.2:38009178-38009197 None:intergenic 25.0%
! CTAATTGTAATCCATGTATA+AGG - chr8.2:38009058-38009077 MS.gene65624:CDS 25.0%
! CTCCTTCAACATAAAAAAAA+AGG - chr8.2:38008992-38009011 MS.gene65624:CDS 25.0%
! TAAATTGTTCGAAAATGCAT+TGG - chr8.2:38008480-38008499 MS.gene65624:CDS 25.0%
!! TAAACATCACTATGAGATTT+TGG - chr8.2:38008579-38008598 MS.gene65624:CDS 25.0%
!! TAGAGGATTATTTAAAGTTC+TGG + chr8.2:38009529-38009548 None:intergenic 25.0%
!! TATTTTCAAATTTGATGAGC+AGG - chr8.2:38009235-38009254 MS.gene65624:intron 25.0%
ACTAATTAAGGTGAAGCTTT+GGG - chr8.2:38008853-38008872 MS.gene65624:CDS 30.0%
TACTAATTAAGGTGAAGCTT+TGG - chr8.2:38008852-38008871 MS.gene65624:CDS 30.0%
TCTGTTGGAACTAGAAATTT+AGG - chr8.2:38009582-38009601 MS.gene65624:intron 30.0%
TGGATGATCATCTAAATTTC+TGG + chr8.2:38009779-38009798 None:intergenic 30.0%
TGTTTATCTTTGCTTTGCTT+TGG - chr8.2:38008759-38008778 MS.gene65624:intron 30.0%
! TGAGCAAATGTATTTTGCAT+GGG + chr8.2:38009988-38010007 None:intergenic 30.0%
!! AAATTTCTAGTTCCAACAGA+TGG + chr8.2:38009582-38009601 None:intergenic 30.0%
!! ATGAGCAGGTTATTTCTTAT+GGG - chr8.2:38009249-38009268 MS.gene65624:intron 30.0%
!!! AATCTCTTCTAAAGTTTTCC+CGG + chr8.2:38008560-38008579 None:intergenic 30.0%
!!! CGCCTTTTTTTTTATGTTGA+AGG + chr8.2:38008997-38009016 None:intergenic 30.0%
AAAGAATCATAATCTGGCAG+AGG + chr8.2:38008640-38008659 None:intergenic 35.0%
CAAAACCTGATTCAATGTTG+TGG + chr8.2:38008615-38008634 None:intergenic 35.0%
CAAGCACTAATCGTATATAG+CGG + chr8.2:38009154-38009173 None:intergenic 35.0%
CATGCTCAGAAATACTTCAA+AGG - chr8.2:38009354-38009373 MS.gene65624:intron 35.0%
CGAAAGAGAAGAAAGAAAGA+AGG - chr8.2:38009388-38009407 MS.gene65624:intron 35.0%
GATATCCACAACATTGAATC+AGG - chr8.2:38008607-38008626 MS.gene65624:CDS 35.0%
TCTTTCTTTCTTCTCTTTCG+TGG + chr8.2:38009388-38009407 None:intergenic 35.0%
TTCTGTCCAATGATAAGAAC+CGG + chr8.2:38008725-38008744 None:intergenic 35.0%
TTTCTTGGACAAATATGCCA+GGG - chr8.2:38009932-38009951 MS.gene65624:CDS 35.0%
! AAAAAAAAGGCGTGAAGCTT+TGG - chr8.2:38009005-38009024 MS.gene65624:CDS 35.0%
! AGAAGTATGGAAAGGGTAAA+TGG - chr8.2:38009277-38009296 MS.gene65624:intron 35.0%
! ATTATCTCTGGTGCTAGTTT+AGG - chr8.2:38008931-38008950 MS.gene65624:CDS 35.0%
! GTGAGCAAATGTATTTTGCA+TGG + chr8.2:38009989-38010008 None:intergenic 35.0%
! TTATCTCTGGTGCTAGTTTA+GGG - chr8.2:38008932-38008951 MS.gene65624:CDS 35.0%
! TTTTGCATGGGAAATGTCAT+AGG + chr8.2:38009976-38009995 None:intergenic 35.0%
!! GATGAGCAGGTTATTTCTTA+TGG - chr8.2:38009248-38009267 MS.gene65624:intron 35.0%
!! TCTAAATTTCTGGTTCCAGA+AGG + chr8.2:38009769-38009788 None:intergenic 35.0%
!!! ATCTCTTCTAAAGTTTTCCC+GGG + chr8.2:38008559-38008578 None:intergenic 35.0%
AAAAAGGGAACTAAGGCTTC+CGG - chr8.2:38008703-38008722 MS.gene65624:CDS 40.0%
AATACCATAAGCCATGTGTC+CGG + chr8.2:38009905-38009924 None:intergenic 40.0%
AGCTGTGAAAAAGGGAACTA+AGG - chr8.2:38008696-38008715 MS.gene65624:CDS 40.0%
ATTGGACAGAAGATGAACAC+AGG - chr8.2:38008734-38008753 MS.gene65624:intron 40.0%
GAAAGAGGAGCTGTGAAAAA+GGG - chr8.2:38008688-38008707 MS.gene65624:CDS 40.0%
GATACAACCAGTCCTTCTAT+AGG + chr8.2:38009661-38009680 None:intergenic 40.0%
GCAACATACATTTCAGTAGG+TGG + chr8.2:38009739-38009758 None:intergenic 40.0%
GCAGCAACATACATTTCAGT+AGG + chr8.2:38009742-38009761 None:intergenic 40.0%
GGCATATTTGTCCAAGAAAC+TGG + chr8.2:38009931-38009950 None:intergenic 40.0%
GTTTATGATGTGCCTTGGAT+CGG - chr8.2:38009820-38009839 MS.gene65624:CDS 40.0%
GTTTCTTGGACAAATATGCC+AGG - chr8.2:38009931-38009950 MS.gene65624:CDS 40.0%
TGAAAGAGGAGCTGTGAAAA+AGG - chr8.2:38008687-38008706 MS.gene65624:CDS 40.0%
TGATCGATGGGAGAAAATTG+CGG - chr8.2:38008525-38008544 MS.gene65624:CDS 40.0%
TTCTTGGACAAATATGCCAG+GGG - chr8.2:38009933-38009952 MS.gene65624:CDS 40.0%
TTTATGATGTGCCTTGGATC+GGG - chr8.2:38009821-38009840 MS.gene65624:CDS 40.0%
! AAAGTTCTGGTACCAACAGA+TGG + chr8.2:38009516-38009535 None:intergenic 40.0%
! ATGGTATTGGACCAGTTTCT+TGG - chr8.2:38009917-38009936 MS.gene65624:CDS 40.0%
! TGGTGCTAGTTTAGGGTTTA+GGG - chr8.2:38008939-38008958 MS.gene65624:CDS 40.0%
!! AAATTTCTGGTTCCAGAAGG+CGG + chr8.2:38009766-38009785 None:intergenic 40.0%
AGAGAGGACCAACCTATAGA+AGG - chr8.2:38009646-38009665 MS.gene65624:intron 45.0%
AGGCTTCCGGTTCTTATCAT+TGG - chr8.2:38008716-38008735 MS.gene65624:CDS 45.0%
ATACCATAAGCCATGTGTCC+GGG + chr8.2:38009904-38009923 None:intergenic 45.0%
ATAGGCGACATCGTGTATTC+TGG + chr8.2:38009418-38009437 None:intergenic 45.0%
ATTTCAGTAGGTGGAGCTTG+AGG + chr8.2:38009730-38009749 None:intergenic 45.0%
CAACCAGTCCTTCTATAGGT+TGG + chr8.2:38009657-38009676 None:intergenic 45.0%
CACTCAGACTCGATTATCTC+TGG - chr8.2:38008919-38008938 MS.gene65624:CDS 45.0%
GATACAACCCGTCTTCCTAT+AGG + chr8.2:38009850-38009869 None:intergenic 45.0%
GGACACATGGCTTATGGTAT+TGG - chr8.2:38009904-38009923 MS.gene65624:CDS 45.0%
GTCATAGGACCCAAGTTCAT+TGG + chr8.2:38009961-38009980 None:intergenic 45.0%
TCGCTTGCTTGACTGGTAAT+TGG + chr8.2:38009456-38009475 None:intergenic 45.0%
TCGGAGTTTATGATGTGCCT+TGG - chr8.2:38009815-38009834 MS.gene65624:CDS 45.0%
TGACTGGTAATTGGTCCTTG+AGG + chr8.2:38009447-38009466 None:intergenic 45.0%
TGGCAAAGCAGCATACATTC+CGG + chr8.2:38009562-38009581 None:intergenic 45.0%
TGGGTAGTAGAAGCTATGAG+TGG - chr8.2:38008443-38008462 MS.gene65624:CDS 45.0%
TGGTCCTTGAGGTTTGACAT+AGG + chr8.2:38009436-38009455 None:intergenic 45.0%
! AGCTTGAGGAGCATAAACTC+CGG + chr8.2:38009716-38009735 None:intergenic 45.0%
! CTGGTGCTAGTTTAGGGTTT+AGG - chr8.2:38008938-38008957 MS.gene65624:CDS 45.0%
!! AATTTCTGGTTCCAGAAGGC+GGG + chr8.2:38009765-38009784 None:intergenic 45.0%
!! AGACGGGTTGTATCTGCTTT+CGG - chr8.2:38009856-38009875 MS.gene65624:CDS 45.0%
!! CAGGTTATTTCTTATGGGCG+TGG - chr8.2:38009254-38009273 MS.gene65624:intron 45.0%
!! GGACTGGTTGTATCTGAAGT+TGG - chr8.2:38009667-38009686 MS.gene65624:intron 45.0%
!! TATGCTGCTTTGCCATCTGT+TGG - chr8.2:38009567-38009586 MS.gene65624:intron 45.0%
!! TCTATAGGTTGGTCCTCTCT+AGG + chr8.2:38009646-38009665 None:intergenic 45.0%
AAAACTCCAGCACGTGGGAT+TGG + chr8.2:38009615-38009634 None:intergenic 50.0%
AAAATTGCGGCTGATGTGCC+CGG - chr8.2:38008538-38008557 MS.gene65624:CDS 50.0%
AAACTCCGAGTCCAGCAAGT+GGG + chr8.2:38009804-38009823 None:intergenic 50.0%
AAAGCAGCATACATTCCGGC+AGG + chr8.2:38009558-38009577 None:intergenic 50.0%
AACAGCATCCTCGGGATGAT+TGG + chr8.2:38008506-38008525 None:intergenic 50.0%
AAGAGCTCAACAAAGCGTGC+TGG - chr8.2:38008664-38008683 MS.gene65624:CDS 50.0%
ACTCCTCAAGCTCCATCTGT+TGG - chr8.2:38009501-38009520 MS.gene65624:intron 50.0%
AGCGAAAAACTCCAGCACGT+GGG + chr8.2:38009620-38009639 None:intergenic 50.0%
CATCGATCAACAGCATCCTC+GGG + chr8.2:38008514-38008533 None:intergenic 50.0%
CCATCGATCAACAGCATCCT+CGG + chr8.2:38008515-38008534 None:intergenic 50.0%
CGAGGATGCTGTTGATCGAT+GGG - chr8.2:38008513-38008532 MS.gene65624:CDS 50.0%
CTTATGGGCGTGGAGAAGTA+TGG - chr8.2:38009264-38009283 MS.gene65624:intron 50.0%
GGACCAACCTATAGAAGGAC+TGG - chr8.2:38009651-38009670 MS.gene65624:intron 50.0%
GTACCAACAGATGGAGCTTG+AGG + chr8.2:38009507-38009526 None:intergenic 50.0%
GTCGCCTATGTCAAACCTCA+AGG - chr8.2:38009429-38009448 MS.gene65624:intron 50.0%
TAAACTCCGAGTCCAGCAAG+TGG + chr8.2:38009805-38009824 None:intergenic 50.0%
TCGGGCAACCTATAGGAAGA+CGG - chr8.2:38009839-38009858 MS.gene65624:CDS 50.0%
TGCAGAATCGCTTGCTTGAC+TGG + chr8.2:38009463-38009482 None:intergenic 50.0%
TGGAGCTTGAGGAGTTTGTG+TGG + chr8.2:38009496-38009515 None:intergenic 50.0%
! ACAAAGCGTGCTGGTGAAAG+AGG - chr8.2:38008673-38008692 MS.gene65624:CDS 50.0%
! GCTTGAGGAGCATAAACTCC+GGG + chr8.2:38009715-38009734 None:intergenic 50.0%
! GGCGTGGAGAAGTATGGAAA+GGG - chr8.2:38009270-38009289 MS.gene65624:intron 50.0%
! GTATCTGAAGTTGGCACTCC+AGG - chr8.2:38009676-38009695 MS.gene65624:intron 50.0%
! GTTTTTCGCTCCTCCTAGAG+AGG - chr8.2:38009630-38009649 MS.gene65624:intron 50.0%
!! ATAGGTTGGTCCTCTCTAGG+AGG + chr8.2:38009643-38009662 None:intergenic 50.0%
!! ATTTCTGGTTCCAGAAGGCG+GGG + chr8.2:38009764-38009783 None:intergenic 50.0%
!! CCAGGGGTACCAATGAACTT+GGG - chr8.2:38009949-38009968 MS.gene65624:CDS 50.0%
AAATTGCGGCTGATGTGCCC+GGG - chr8.2:38008539-38008558 MS.gene65624:CDS 55.0%
AACTCCGAGTCCAGCAAGTG+GGG + chr8.2:38009803-38009822 None:intergenic 55.0%
AATCCTCCAATCCCACGTGC+TGG - chr8.2:38009606-38009625 MS.gene65624:intron 55.0%
CCAGGAATTCTGACTGCTCC+CGG - chr8.2:38009694-38009713 MS.gene65624:CDS 55.0%
CCCAAGTTCATTGGTACCCC+TGG + chr8.2:38009952-38009971 None:intergenic 55.0%
CCGAGGATGCTGTTGATCGA+TGG - chr8.2:38008512-38008531 MS.gene65624:CDS 55.0%
CCTATAGGTTGCCCGATCCA+AGG + chr8.2:38009835-38009854 None:intergenic 55.0%
CCTTGGATCGGGCAACCTAT+AGG - chr8.2:38009832-38009851 MS.gene65624:CDS 55.0%
CGGGCAACCTATAGGAAGAC+GGG - chr8.2:38009840-38009859 MS.gene65624:CDS 55.0%
GAGCGAAAAACTCCAGCACG+TGG + chr8.2:38009621-38009640 None:intergenic 55.0%
TATCTGCTTTCGGCGCTGCA+GGG - chr8.2:38009866-38009885 MS.gene65624:CDS 55.0%
! AATCCTCTAGCTCCACCTGC+CGG - chr8.2:38009540-38009559 MS.gene65624:intron 55.0%
! GGGCGTGGAGAAGTATGGAA+AGG - chr8.2:38009269-38009288 MS.gene65624:intron 55.0%
! TGCATTGGCCAATCATCCCG+AGG - chr8.2:38008495-38008514 MS.gene65624:CDS 55.0%
!! GCCAGGGGTACCAATGAACT+TGG - chr8.2:38009948-38009967 MS.gene65624:CDS 55.0%
ACTCCAGCACGTGGGATTGG+AGG + chr8.2:38009612-38009631 None:intergenic 60.0%
ATTCCGGCAGGTGGAGCTAG+AGG + chr8.2:38009546-38009565 None:intergenic 60.0%
CCGGGAGCAGTCAGAATTCC+TGG + chr8.2:38009697-38009716 None:intergenic 60.0%
GATCATCCAGCCCCACTTGC+TGG - chr8.2:38009790-38009809 MS.gene65624:CDS 60.0%
GCAGCATACATTCCGGCAGG+TGG + chr8.2:38009555-38009574 None:intergenic 60.0%
GCAGGGTTTCCTTCTGCTCC+CGG - chr8.2:38009883-38009902 MS.gene65624:CDS 60.0%
GCTCCCGGACACATGGCTTA+TGG - chr8.2:38009898-38009917 MS.gene65624:CDS 60.0%
GTATCTGCTTTCGGCGCTGC+AGG - chr8.2:38009865-38009884 MS.gene65624:CDS 60.0%
TATGTTGCTGCCCCGCCTTC+TGG - chr8.2:38009751-38009770 MS.gene65624:CDS 60.0%
TCCTTCTGCTCCCGGACACA+TGG - chr8.2:38009891-38009910 MS.gene65624:CDS 60.0%
GCCATGTGTCCGGGAGCAGA+AGG + chr8.2:38009895-38009914 None:intergenic 65.0%
! CCAGCCCCACTTGCTGGACT+CGG - chr8.2:38009796-38009815 MS.gene65624:CDS 65.0%
CCGAGTCCAGCAAGTGGGGC+TGG + chr8.2:38009799-38009818 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38008433 38010011 38008433 ID=MS.gene65624
chr8.2 mRNA 38008433 38010011 38008433 ID=MS.gene65624.t1;Parent=MS.gene65624
chr8.2 exon 38009689 38010011 38009689 ID=MS.gene65624.t1.exon1;Parent=MS.gene65624.t1
chr8.2 CDS 38009689 38010011 38009689 ID=cds.MS.gene65624.t1;Parent=MS.gene65624.t1
chr8.2 exon 38008841 38009187 38008841 ID=MS.gene65624.t1.exon2;Parent=MS.gene65624.t1
chr8.2 CDS 38008841 38009187 38008841 ID=cds.MS.gene65624.t1;Parent=MS.gene65624.t1
chr8.2 exon 38008433 38008746 38008433 ID=MS.gene65624.t1.exon3;Parent=MS.gene65624.t1
chr8.2 CDS 38008433 38008746 38008433 ID=cds.MS.gene65624.t1;Parent=MS.gene65624.t1
Gene Sequence

>MS.gene65624

ATGGATGAAGTGGGTAGTAGAAGCTATGAGTGGAGCAGCGAGCAGAATAAATTGTTCGAAAATGCATTGGCCAATCATCCCGAGGATGCTGTTGATCGATGGGAGAAAATTGCGGCTGATGTGCCCGGGAAAACTTTAGAAGAGATTAAACATCACTATGAGATTTTGGTGTATGATATCCACAACATTGAATCAGGTTTTGTGCCTCTGCCAGATTATGATTCTTTTTCAAAGAGCTCAACAAAGCGTGCTGGTGAAAGAGGAGCTGTGAAAAAGGGAACTAAGGCTTCCGGTTCTTATCATTGGACAGAAGATGAACACAGGTTTGTTTATCTTTGCTTTGCTTTGGTAACTTAATTGTTTTTAGATTGTTATTATTTACAACTTACATGGCTGTTAAAAACATAATAATGATATGATACTAATTAAGGTGAAGCTTTGGGTCTACTAGCGTTGACTTGAGACATTAGAGTGTGCTCTATCAAGCACTCAGACTCGATTATCTCTGGTGCTAGTTTAGGGTTTAGGGTTTAGAGTTTCTTTAATTAGTTTCAATGGACTCCTTCAACATAAAAAAAAAGGCGTGAAGCTTTGGTACAACTTACTTTTCATAATTGAAAAAGTGCTAATTGTAATCCATGTATAAGGAATTATTGTATCTTGAATTATCATGTACATTATACATCAAATAGCTTAATTATTTCTAGAGGTTTATAGTCCGCTATATACGATTAGTGCTTGACCGTATCAATATGTGTGCATTTTACATGCATGTTTCCATATTATTAGTATTGTTTTTAAGTATTTTCAAATTTGATGAGCAGGTTATTTCTTATGGGCGTGGAGAAGTATGGAAAGGGTAAATGGCGAAAAATATCAGAGAATTATGTGGTGACAAGAACACATACACAAGTAGCAAGTCATGCTCAGAAATACTTCAAAGGTCTGAATTCCACGAAAGAGAAGAAAGAAAGAAGGCGATCCAGAATACACGATGTCGCCTATGTCAAACCTCAAGGACCAATTACCAGTCAAGCAAGCGATTCTGCAAGACAATCAGCCACACAAACTCCTCAAGCTCCATCTGTTGGTACCAGAACTTTAAATAATCCTCTAGCTCCACCTGCCGGAATGTATGCTGCTTTGCCATCTGTTGGAACTAGAAATTTAGGTAATCCTCCAATCCCACGTGCTGGAGTTTTTCGCTCCTCCTAGAGAGGACCAACCTATAGAAGGACTGGTTGTATCTGAAGTTGGCACTCCAGGAATTCTGACTGCTCCCGGAGTTTATGCTCCTCAAGCTCCACCTACTGAAATGTATGTTGCTGCCCCGCCTTCTGGAACCAGAAATTTAGATGATCATCCAGCCCCACTTGCTGGACTCGGAGTTTATGATGTGCCTTGGATCGGGCAACCTATAGGAAGACGGGTTGTATCTGCTTTCGGCGCTGCAGGGTTTCCTTCTGCTCCCGGACACATGGCTTATGGTATTGGACCAGTTTCTTGGACAAATATGCCAGGGGTACCAATGAACTTGGGTCCTATGACATTTCCCATGCAAAATACATTTGCTCACTAA
Protein sequence

>MS.gene65624.t1

MDEVGSRSYEWSSEQNKLFENALANHPEDAVDRWEKIAADVPGKTLEEIKHHYEILVYDIHNIESGFVPLPDYDSFSKSSTKRAGERGAVKKGTKASGSYHWTEDEHRLFLMGVEKYGKGKWRKISENYVVTRTHTQVASHAQKYFKGLNSTKEKKERRRSRIHDVAYVKPQGPITSQASDSARQSATQTPQAPSVGTRTLNNPLAPPAGMYAALPSVGTRNLGILTAPGVYAPQAPPTEMYVAAPPSGTRNLDDHPAPLAGLGVYDVPWIGQPIGRRVVSAFGAAGFPSAPGHMAYGIGPVSWTNMPGVPMNLGPMTFPMQNTFAH