AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65652

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65652.t1 MTR_1g040077 66.225 151 42 2 37 186 171 313 3.39e-58 195
MS.gene65652.t1 MTR_1g040077 88.095 42 5 0 205 246 493 534 4.95e-19 86.7
MS.gene65652.t1 MTR_1g040073 66.225 151 42 2 37 186 171 313 2.62e-57 195
MS.gene65652.t1 MTR_1g040073 92.982 57 4 0 205 261 493 549 1.24e-30 120
MS.gene65652.t1 MTR_2g080220 41.667 228 81 8 2 185 141 360 6.50e-39 144
MS.gene65652.t1 MTR_2g080220 73.684 57 15 0 205 261 562 618 4.70e-22 95.5
MS.gene65652.t1 MTR_8g467150 39.819 221 82 7 1 185 144 349 3.50e-33 127
MS.gene65652.t1 MTR_8g467150 87.719 57 7 0 205 261 509 565 5.53e-28 112
MS.gene65652.t1 MTR_2g072740 89.474 57 6 0 205 261 68 124 9.97e-30 112
MS.gene65652.t1 MTR_8g037700 32.735 223 102 6 1 180 171 388 6.03e-24 101
MS.gene65652.t1 MTR_8g037700 66.071 56 19 0 205 260 591 646 1.25e-18 85.5
MS.gene65652.t1 MTR_4g109010 31.507 219 99 6 7 180 169 381 9.16e-23 97.8
MS.gene65652.t1 MTR_4g109010 63.158 57 21 0 205 261 597 653 2.73e-19 87.4
MS.gene65652.t1 MTR_2g030310 68.421 57 18 0 205 261 581 637 1.80e-20 90.9
MS.gene65652.t1 MTR_2g030310 28.571 210 111 7 2 175 156 362 4.74e-18 84.0
MS.gene65652.t1 MTR_2g030310 68.421 57 18 0 205 261 581 637 1.86e-20 90.9
MS.gene65652.t1 MTR_2g030310 28.571 210 111 7 2 175 156 362 3.94e-18 84.0
MS.gene65652.t1 MTR_4g061833 30.986 213 95 8 9 177 1 205 5.64e-18 83.6
MS.gene65652.t1 MTR_4g061833 62.500 56 21 0 205 260 409 464 7.07e-18 83.2
MS.gene65652.t1 MTR_4g061833 62.500 56 21 0 205 260 560 615 6.71e-18 83.6
MS.gene65652.t1 MTR_4g061833 30.841 214 96 8 8 177 151 356 7.52e-18 83.2
MS.gene65652.t1 MTR_4g061930 62.500 56 21 0 205 260 560 615 6.71e-18 83.6
MS.gene65652.t1 MTR_4g061930 30.841 214 96 8 8 177 151 356 7.52e-18 83.2
MS.gene65652.t1 MTR_2g096160 30.415 217 96 8 8 177 149 357 1.19e-17 82.8
MS.gene65652.t1 MTR_2g096160 58.929 56 23 0 205 260 563 618 2.40e-16 79.0
MS.gene65652.t1 MTR_7g015510 64.912 57 20 0 205 261 593 649 5.82e-17 80.5
MS.gene65652.t1 MTR_7g015550 64.912 57 20 0 205 261 593 649 5.93e-17 80.5
MS.gene65652.t1 MTR_4g111925 63.158 57 21 0 205 261 602 658 6.31e-17 80.5
MS.gene65652.t1 MTR_7g015390 61.404 57 22 0 205 261 593 649 8.09e-17 80.1
MS.gene65652.t1 MTR_6g015805 61.404 57 22 0 205 261 596 652 8.51e-17 80.1
MS.gene65652.t1 MTR_7g073660 63.158 57 21 0 205 261 602 658 1.08e-16 79.7
MS.gene65652.t1 MTR_7g015420 63.158 57 21 0 205 261 584 640 1.20e-16 79.7
MS.gene65652.t1 MTR_6g048090 61.404 57 21 1 205 260 556 612 1.49e-16 79.3
MS.gene65652.t1 MTR_7g015760 64.912 57 20 0 205 261 101 157 1.62e-16 78.2
MS.gene65652.t1 MTR_7g015760 64.912 57 20 0 205 261 101 157 1.72e-16 78.2
MS.gene65652.t1 MTR_1g080740 62.712 59 20 1 205 261 582 640 4.97e-16 77.8
MS.gene65652.t1 MTR_1g080740 31.081 148 83 6 34 177 224 356 1.10e-12 68.2
MS.gene65652.t1 MTR_7g015750 61.404 57 22 0 205 261 105 161 5.20e-16 77.0
MS.gene65652.t1 MTR_7g015310 61.818 55 21 0 205 259 585 639 5.32e-16 77.8
MS.gene65652.t1 MTR_1g100110 59.649 57 22 1 205 260 560 616 6.36e-16 77.4
MS.gene65652.t1 MTR_7g015670 60.714 56 22 0 205 260 596 651 7.86e-16 77.4
MS.gene65652.t1 MTR_4g095032 58.929 56 23 0 205 260 499 554 2.85e-15 75.5
MS.gene65652.t1 MTR_5g047120 58.929 56 23 0 205 260 574 629 5.04e-15 75.1
MS.gene65652.t1 MTR_7g115300 61.667 60 19 2 205 261 587 645 7.06e-15 74.3
MS.gene65652.t1 MTR_7g115300 26.667 225 101 9 4 177 155 366 7.47e-15 74.3
MS.gene65652.t1 MTR_5g047070 58.929 56 23 0 205 260 333 388 7.65e-15 74.3
MS.gene65652.t1 MTR_7g018130 59.649 57 23 0 205 261 101 157 8.65e-15 73.2
MS.gene65652.t1 MTR_5g075650 59.649 57 23 0 205 261 676 732 1.24e-14 73.9
MS.gene65652.t1 MTR_7g015240 58.182 55 23 0 205 259 582 636 1.59e-14 73.6
MS.gene65652.t1 MTR_7g015250 57.407 54 23 0 205 258 579 632 2.05e-14 73.2
MS.gene65652.t1 MTR_7g015230 56.364 55 24 0 205 259 595 649 2.93e-14 72.8
MS.gene65652.t1 MTR_4g095042 57.143 56 24 0 205 260 558 613 3.41e-14 72.4
MS.gene65652.t1 MTR_5g047060 59.016 61 21 2 205 261 581 641 4.28e-14 72.0
MS.gene65652.t1 MTR_5g047110 57.377 61 22 1 205 261 565 625 4.54e-14 72.0
MS.gene65652.t1 MTR_7g015620 65.306 49 17 0 213 261 562 610 4.56e-14 72.0
MS.gene65652.t1 MTR_7g015280 55.556 54 24 0 205 258 575 628 5.39e-14 72.0
MS.gene65652.t1 MTR_7g015800 57.627 59 23 1 205 261 101 159 6.41e-14 70.9
MS.gene65652.t1 MTR_7g015800 57.627 59 23 1 205 261 101 159 6.69e-14 70.9
MS.gene65652.t1 MTR_4g052290 55.556 54 24 0 205 258 588 641 2.14e-13 70.1
MS.gene65652.t1 MTR_4g095045 57.895 57 23 1 205 260 366 422 2.19e-13 70.1
MS.gene65652.t1 MTR_8g016120 57.407 54 23 0 205 258 465 518 3.09e-13 69.3
MS.gene65652.t1 MTR_2g100290 55.556 54 24 0 205 258 467 520 3.37e-13 69.3
MS.gene65652.t1 MTR_8g016120 57.407 54 23 0 205 258 465 518 3.52e-13 69.3
MS.gene65652.t1 MTR_8g067735 59.259 54 21 1 205 258 421 473 5.40e-13 68.9
MS.gene65652.t1 MTR_7g074610 57.407 54 23 0 205 258 158 211 5.42e-13 68.6
MS.gene65652.t1 MTR_4g095022 27.434 226 103 7 8 180 146 363 9.81e-13 67.8
MS.gene65652.t1 MTR_8g067930 57.407 54 22 1 205 258 402 454 1.01e-12 68.2
MS.gene65652.t1 MTR_8g067690 57.407 54 22 1 205 258 418 470 1.73e-12 67.4
MS.gene65652.t1 MTR_8g067720 57.407 54 22 1 205 258 401 453 2.53e-12 66.6
MS.gene65652.t1 MTR_8g101670 57.407 54 23 0 205 258 452 505 3.37e-12 66.6
MS.gene65652.t1 MTR_8g101670 57.407 54 23 0 205 258 464 517 3.79e-12 66.2
MS.gene65652.t1 MTR_8g101670 57.407 54 23 0 205 258 464 517 3.87e-12 66.2
MS.gene65652.t1 MTR_3g064090 55.556 54 24 0 205 258 389 442 4.24e-12 66.2
MS.gene65652.t1 MTR_3g062590 54.386 57 26 0 205 261 682 738 5.33e-12 65.9
MS.gene65652.t1 MTR_8g008820 55.172 58 22 1 205 258 142 199 6.23e-12 65.5
MS.gene65652.t1 MTR_8g067630 55.556 54 23 1 205 258 350 402 6.32e-12 65.5
MS.gene65652.t1 MTR_4g115120 53.571 56 25 1 205 260 181 235 7.13e-12 65.1
MS.gene65652.t1 MTR_2g028580 54.386 57 26 0 205 261 699 755 9.05e-12 65.5
MS.gene65652.t1 MTR_2g028580 54.386 57 26 0 205 261 687 743 9.90e-12 65.1
MS.gene65652.t1 MTR_7g063010 53.571 56 26 0 205 260 400 455 1.06e-11 65.1
MS.gene65652.t1 MTR_3g462840 49.123 57 29 0 205 261 134 190 1.13e-11 64.7
MS.gene65652.t1 MTR_4g095012 25.714 245 121 6 8 199 145 381 1.19e-11 65.1
MS.gene65652.t1 MTR_3g011730 48.148 54 28 0 205 258 372 425 1.73e-11 64.3
MS.gene65652.t1 MTR_2g022810 55.357 56 23 1 205 258 140 195 1.77e-11 63.9
MS.gene65652.t1 MTR_6g011330 52.632 57 26 1 205 261 203 258 2.01e-11 63.9
MS.gene65652.t1 MTR_3g062570 52.632 57 27 0 205 261 429 485 2.07e-11 63.9
MS.gene65652.t1 MTR_3g062570 52.632 57 27 0 205 261 565 621 2.11e-11 64.3
MS.gene65652.t1 MTR_8g070880 51.852 54 26 0 205 258 689 742 2.83e-11 63.9
MS.gene65652.t1 MTR_5g035910 51.786 56 27 0 205 260 407 462 2.92e-11 63.5
MS.gene65652.t1 MTR_7g062950 53.704 54 25 0 205 258 402 455 3.18e-11 63.5
MS.gene65652.t1 MTR_3g062570 52.632 57 27 0 205 261 680 736 3.32e-11 63.5
MS.gene65652.t1 MTR_2g075250 50.909 55 25 1 206 258 727 781 4.29e-11 63.2
MS.gene65652.t1 MTR_3g115490 52.727 55 25 1 205 259 152 205 4.52e-11 62.8
MS.gene65652.t1 MTR_2g075250 50.909 55 25 1 206 258 632 686 4.53e-11 63.2
MS.gene65652.t1 MTR_5g092120 36.029 136 75 4 131 261 62 190 4.65e-11 62.8
MS.gene65652.t1 MTR_3g064080 53.704 54 25 0 205 258 386 439 4.90e-11 62.8
MS.gene65652.t1 MTR_7g063030 48.214 56 29 0 205 260 404 459 4.90e-11 63.2
MS.gene65652.t1 MTR_5g013610 52.542 59 25 1 205 260 130 188 5.51e-11 62.4
MS.gene65652.t1 MTR_4g133920 50.847 59 24 1 205 258 93 151 5.78e-11 62.0
MS.gene65652.t1 MTR_7g063030 48.214 56 29 0 205 260 398 453 6.05e-11 62.8
MS.gene65652.t1 MTR_8g073560 47.458 59 29 1 205 261 148 206 6.43e-11 62.4
MS.gene65652.t1 MTR_8g073560 47.458 59 29 1 205 261 162 220 6.61e-11 61.6
MS.gene65652.t1 MTR_8g068050 57.407 54 22 1 205 258 417 469 6.71e-11 62.4
MS.gene65652.t1 MTR_5g099130 52.632 57 25 1 205 259 159 215 7.43e-11 62.0
MS.gene65652.t1 MTR_5g099130 52.632 57 25 1 205 259 170 226 8.66e-11 62.0
MS.gene65652.t1 MTR_8g070910 50.000 54 27 0 205 258 336 389 8.70e-11 62.0
MS.gene65652.t1 MTR_5g099130 52.632 57 25 1 205 259 166 222 8.75e-11 62.0
MS.gene65652.t1 MTR_8g068050 57.407 54 22 1 205 258 307 359 9.20e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65652.t1 AT5G54380 41.935 217 73 8 7 179 146 353 1.71e-37 140
MS.gene65652.t1 AT5G54380 73.684 57 15 0 205 261 570 626 5.43e-22 95.5
MS.gene65652.t1 AT2G21480 31.839 223 99 7 2 180 169 382 1.89e-22 96.7
MS.gene65652.t1 AT2G21480 66.071 56 19 0 205 260 585 640 4.18e-20 90.1
MS.gene65652.t1 AT5G59700 31.429 210 106 6 8 180 138 346 3.35e-20 90.1
MS.gene65652.t1 AT5G59700 66.071 56 19 0 205 260 542 597 3.95e-19 87.0
MS.gene65652.t1 AT4G39110 30.942 223 101 7 2 180 170 383 4.39e-20 89.7
MS.gene65652.t1 AT4G39110 62.500 56 21 0 205 260 586 641 1.71e-19 88.2
MS.gene65652.t1 AT1G30570 64.912 57 20 0 205 261 580 636 2.62e-19 87.8
MS.gene65652.t1 AT1G30570 28.829 222 101 8 2 177 158 368 9.80e-16 77.0
MS.gene65652.t1 AT5G61350 31.818 220 99 7 4 180 149 360 1.79e-18 85.1
MS.gene65652.t1 AT5G61350 61.905 63 18 2 205 261 585 647 8.76e-17 80.1
MS.gene65652.t1 AT5G24010 63.158 57 21 0 205 261 549 605 2.60e-18 84.7
MS.gene65652.t1 AT3G46290 62.500 56 21 0 205 260 545 600 2.76e-18 84.7
MS.gene65652.t1 AT3G46290 29.902 204 90 6 4 163 135 329 6.51e-16 77.8
MS.gene65652.t1 AT5G39000 60.000 60 21 1 205 261 579 638 6.78e-18 83.6
MS.gene65652.t1 AT5G38990 58.333 60 22 1 205 261 586 645 1.95e-17 82.0
MS.gene65652.t1 AT2G39360 60.345 58 22 1 205 261 547 604 2.53e-16 79.0
MS.gene65652.t1 AT3G51550 61.404 57 22 0 205 261 597 653 3.96e-16 78.2
MS.gene65652.t1 AT3G51550 24.779 226 108 6 7 179 156 372 2.24e-14 73.2
MS.gene65652.t1 AT2G23200 55.357 56 25 0 206 261 549 604 1.19e-15 77.0
MS.gene65652.t1 AT3G04690 59.649 57 23 0 205 261 577 633 8.54e-15 74.3
MS.gene65652.t1 AT5G28680 56.140 57 25 0 205 261 581 637 3.44e-14 72.4
MS.gene65652.t1 AT5G28680 32.867 143 84 4 38 176 224 358 5.57e-11 62.8
MS.gene65652.t1 AT5G28680 56.140 57 25 0 205 261 581 637 3.44e-14 72.4
MS.gene65652.t1 AT5G28680 32.867 143 84 4 38 176 224 358 5.57e-11 62.8
MS.gene65652.t1 AT5G56790 59.259 54 22 0 205 258 450 503 9.90e-14 71.2
MS.gene65652.t1 AT4G29990 57.143 56 24 0 205 260 633 688 1.81e-13 70.5
MS.gene65652.t1 AT4G29990 57.143 56 24 0 205 260 633 688 1.98e-13 70.1
MS.gene65652.t1 AT5G01020 57.407 54 23 0 205 258 136 189 2.37e-13 69.7
MS.gene65652.t1 AT1G79620 55.556 54 24 0 205 258 698 751 3.79e-13 69.3
MS.gene65652.t1 AT1G79620 55.556 54 24 0 205 258 698 751 3.87e-13 69.3
MS.gene65652.t1 AT1G79620 55.556 54 24 0 205 258 724 777 4.14e-13 69.3
MS.gene65652.t1 AT1G67720 59.649 57 22 1 205 260 666 722 9.17e-13 68.2
MS.gene65652.t1 AT5G54590 54.386 57 26 0 205 261 173 229 1.02e-12 67.8
MS.gene65652.t1 AT5G54590 54.386 57 26 0 205 261 173 229 1.02e-12 67.8
MS.gene65652.t1 AT5G54590 54.386 57 26 0 205 261 173 229 1.02e-12 67.8
MS.gene65652.t1 AT2G37710 44.737 76 40 1 185 258 386 461 1.51e-12 67.4
MS.gene65652.t1 AT4G17660 57.407 54 23 0 205 258 160 213 2.46e-12 66.6
MS.gene65652.t1 AT1G07570 54.386 57 24 1 205 259 22 78 3.30e-12 65.5
MS.gene65652.t1 AT3G13690 55.556 54 24 0 205 258 471 524 4.46e-12 66.2
MS.gene65652.t1 AT3G13690 55.556 54 24 0 205 258 471 524 4.46e-12 66.2
MS.gene65652.t1 AT3G13690 55.556 54 24 0 205 258 471 524 4.46e-12 66.2
MS.gene65652.t1 AT5G54590 53.571 56 26 0 205 260 173 228 4.65e-12 64.7
MS.gene65652.t1 AT1G07570 54.386 57 24 1 205 259 149 205 6.43e-12 65.5
MS.gene65652.t1 AT1G07570 54.386 57 24 1 205 259 138 194 6.91e-12 65.1
MS.gene65652.t1 AT1G07570 54.386 57 24 1 205 259 138 194 6.91e-12 65.1
MS.gene65652.t1 AT1G07570 54.386 57 24 1 205 259 138 194 6.91e-12 65.1
MS.gene65652.t1 AT5G47070 55.172 58 22 1 205 258 153 210 8.17e-12 65.1
MS.gene65652.t1 AT5G47850 63.043 46 17 0 213 258 523 568 9.98e-12 65.1
MS.gene65652.t1 AT1G55200 55.556 54 24 0 205 258 439 492 1.16e-11 65.1
MS.gene65652.t1 AT1G55200 55.556 54 24 0 205 258 439 492 1.16e-11 65.1
MS.gene65652.t1 AT1G55200 55.556 54 24 0 205 258 439 492 1.16e-11 65.1
MS.gene65652.t1 AT1G55200 55.556 54 24 0 205 258 439 492 1.16e-11 65.1
MS.gene65652.t1 AT1G06840 52.632 57 27 0 205 261 685 741 1.61e-11 64.7
MS.gene65652.t1 AT2G19190 51.786 56 27 0 205 260 633 688 1.93e-11 64.3
MS.gene65652.t1 AT2G19230 50.000 56 28 0 205 260 629 684 2.09e-11 64.3
MS.gene65652.t1 AT1G29720 48.214 56 29 0 206 261 734 789 2.21e-11 64.3
MS.gene65652.t1 AT3G45390 52.632 57 27 0 205 261 411 467 2.34e-11 63.9
MS.gene65652.t1 AT2G17220 54.386 57 24 1 205 259 154 210 2.35e-11 63.5
MS.gene65652.t1 AT2G19230 50.000 56 28 0 205 260 628 683 2.39e-11 63.9
MS.gene65652.t1 AT2G17220 54.386 57 24 1 205 259 155 211 2.56e-11 63.5
MS.gene65652.t1 AT5G37450 52.632 57 27 0 205 261 488 544 2.80e-11 63.9
MS.gene65652.t1 AT5G56460 50.000 54 27 0 205 258 146 199 3.00e-11 63.5
MS.gene65652.t1 AT2G28930 55.357 56 23 1 205 258 139 194 3.14e-11 63.2
MS.gene65652.t1 AT2G28930 55.357 56 23 1 205 258 142 197 3.15e-11 63.2
MS.gene65652.t1 AT2G28930 55.357 56 23 1 205 258 150 205 3.35e-11 63.2
MS.gene65652.t1 AT1G72540 39.216 102 60 1 159 258 103 204 3.59e-11 63.2
MS.gene65652.t1 AT2G19210 42.857 77 42 1 186 260 614 690 3.60e-11 63.5
MS.gene65652.t1 AT5G37450 52.632 57 27 0 205 261 667 723 3.65e-11 63.5
MS.gene65652.t1 AT5G37450 52.632 57 27 0 205 261 715 771 3.97e-11 63.5
MS.gene65652.t1 AT5G37450 52.632 57 27 0 205 261 691 747 4.06e-11 63.5
MS.gene65652.t1 AT3G55450 51.724 58 25 1 205 259 131 188 4.81e-11 62.8
MS.gene65652.t1 AT5G49780 51.852 54 26 0 205 258 594 647 5.37e-11 63.2
MS.gene65652.t1 AT1G56140 50.000 56 28 0 206 261 754 809 5.59e-11 63.2
MS.gene65652.t1 AT3G55450 51.724 58 25 1 205 259 168 225 5.99e-11 62.4
MS.gene65652.t1 AT4G02410 40.476 84 48 1 177 258 386 469 6.15e-11 62.8
MS.gene65652.t1 AT1G05700 51.786 56 27 0 205 260 629 684 6.21e-11 62.8
MS.gene65652.t1 AT5G49780 51.852 54 26 0 205 258 715 768 6.23e-11 62.8
MS.gene65652.t1 AT5G49780 51.852 54 26 0 205 258 693 746 6.38e-11 62.8
MS.gene65652.t1 AT3G53810 43.421 76 41 1 185 258 386 461 6.57e-11 62.8
MS.gene65652.t1 AT1G05700 51.786 56 27 0 205 260 660 715 6.62e-11 62.8
MS.gene65652.t1 AT1G56145 49.057 53 27 0 206 258 748 800 8.20e-11 62.4
MS.gene65652.t1 AT1G56145 49.057 53 27 0 206 258 645 697 8.40e-11 62.4
MS.gene65652.t1 AT1G56120 50.000 56 28 0 206 261 753 808 9.19e-11 62.4
MS.gene65652.t1 AT4G20450 49.123 57 28 1 205 260 651 707 9.27e-11 62.4
MS.gene65652.t1 AT1G56130 50.000 56 28 0 206 261 556 611 9.28e-11 62.4
MS.gene65652.t1 AT4G20450 49.123 57 28 1 205 260 651 707 9.59e-11 62.4
MS.gene65652.t1 AT5G60320 50.877 57 28 0 205 261 408 464 9.68e-11 62.0
MS.gene65652.t1 AT1G56130 50.000 56 28 0 206 261 607 662 9.77e-11 62.4
MS.gene65652.t1 AT1G56120 50.000 56 28 0 206 261 605 660 9.85e-11 62.4

Find 50 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGACGGAGAACTGATTT+TGG 0.264143 5.2:+47556369 MS.gene65652:CDS
TGATGCAATTCTCAAGGTTC+TGG 0.288990 5.2:-47555544 None:intergenic
CAATTCAATGGACAACAATA+TGG 0.289373 5.2:+47555656 MS.gene65652:CDS
CGAACAAAATACACAAAGTT+TGG 0.308692 5.2:-47555609 None:intergenic
AAGATGAAATCCTTGTGATA+TGG 0.378531 5.2:-47555756 None:intergenic
GATTTCATCTTCAACTCCTT+AGG 0.380589 5.2:+47555768 MS.gene65652:CDS
TCTCTCAGCAGTCATCTCTA+TGG 0.399364 5.2:+47556415 MS.gene65652:CDS
CAACGCCTTATGATATGCTT+AGG 0.400527 5.2:+47556466 MS.gene65652:CDS
TTCAAGTAACAAAATGATAT+AGG 0.402951 5.2:-47556064 None:intergenic
CTTGATCTTTCATCCAAAAC+CGG 0.406783 5.2:+47555720 MS.gene65652:CDS
AAGACTTTGTTCCTTTGTCT+TGG 0.410629 5.2:+47556440 MS.gene65652:CDS
ATTTCATCTTCAACTCCTTA+GGG 0.419565 5.2:+47555769 MS.gene65652:CDS
AACGCCTTATGATATGCTTA+GGG 0.449154 5.2:+47556467 MS.gene65652:CDS
GTTACTTGAAAAGTCGTATG+AGG 0.459296 5.2:+47556077 MS.gene65652:CDS
CACATGGATCCGATCTAAGT+TGG 0.461507 5.2:+47555571 MS.gene65652:CDS
CAACAAGAGTATATTGCAGC+AGG 0.464779 5.2:-47555471 None:intergenic
TGACATCATTAGCAATTCAA+TGG 0.488938 5.2:+47555644 MS.gene65652:CDS
TCGTATGAGGAAGAAGAAAT+CGG 0.504343 5.2:+47556090 MS.gene65652:CDS
AGATGAAATCCTTGTGATAT+GGG 0.510559 5.2:-47555755 None:intergenic
AGCTCCACCAGCTCCACCAT+TGG 0.515890 5.2:+47555509 MS.gene65652:CDS
GTTTACAAGTACATGGCCAA+TGG 0.518041 5.2:+47556391 MS.gene65652:CDS
CTTTGAGTGACACGCTTGGA+AGG 0.525215 5.2:+47555370 MS.gene65652:CDS
ATGACTGCTGAGAGATCCAT+TGG 0.529561 5.2:-47556407 None:intergenic
ACATGGATCCGATCTAAGTT+GGG 0.535311 5.2:+47555572 MS.gene65652:CDS
ATATGCTTAGGGGCAGCAAA+GGG 0.535890 5.2:+47556478 MS.gene65652:CDS
TGACTTTGATGCAATTCTCA+AGG 0.538969 5.2:-47555550 None:intergenic
TTGATCTTTCATCCAAAACC+GGG 0.545049 5.2:+47555721 MS.gene65652:CDS
CTGCCCCTAAGCATATCATA+AGG 0.547715 5.2:-47556471 None:intergenic
GCATAAACCAATGGTGGAGC+TGG 0.549729 5.2:-47555516 None:intergenic
GATATGCTTAGGGGCAGCAA+AGG 0.551967 5.2:+47556477 MS.gene65652:CDS
ATTGAAGACCCAACTTAGAT+CGG 0.560273 5.2:-47555580 None:intergenic
CACTTTCAAAGTGTTCCCTA+AGG 0.561402 5.2:-47555784 None:intergenic
TCAACTTTGAGTGACACGCT+TGG 0.561942 5.2:+47555366 MS.gene65652:CDS
TAAACCAATGGTGGAGCTGG+TGG 0.567667 5.2:-47555513 None:intergenic
AATTATGCTCTGTTAAGCTC+CGG 0.572418 5.2:-47556518 None:intergenic
GTGACACGCTTGGAAGGACA+TGG 0.582175 5.2:+47555376 MS.gene65652:CDS
TACAACAAGATCAAAACTAG+TGG 0.586227 5.2:-47555439 None:intergenic
GTAGGTTGTTGTAATGAAGA+CGG 0.592581 5.2:+47556355 MS.gene65652:intron
TGGTATAGAGATAACTTCGA+TGG 0.607100 5.2:-47555236 None:intergenic
ATATGGGACATCAAGTCCCC+CGG 0.610327 5.2:-47555739 None:intergenic
GCTGTAGCATAAACCAATGG+TGG 0.616913 5.2:-47555522 None:intergenic
CTGGCTGTAGCATAAACCAA+TGG 0.639190 5.2:-47555525 None:intergenic
AGAATTGCATCAAAGTCACA+TGG 0.644938 5.2:+47555555 MS.gene65652:CDS
AGGCGTTGCTTCCAAGACAA+AGG 0.649937 5.2:-47556451 None:intergenic
GATCTTTCATCCAAAACCGG+GGG 0.651729 5.2:+47555723 MS.gene65652:CDS
AACAAGAGTATATTGCAGCA+GGG 0.655743 5.2:-47555470 None:intergenic
TGATCTTTCATCCAAAACCG+GGG 0.664718 5.2:+47555722 MS.gene65652:CDS
ACTTGATGTCCCATATCACA+AGG 0.689755 5.2:+47555746 MS.gene65652:CDS
ACGCCTTATGATATGCTTAG+GGG 0.696060 5.2:+47556468 MS.gene65652:CDS
TGACACGCTTGGAAGGACAT+GGG 0.697683 5.2:+47555377 MS.gene65652:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGTTCATAAAAACTTGA+TGG - chr5.2:47555301-47555320 None:intergenic 20.0%
!! ATAAAGGAAAACTAGAAAAT+GGG + chr5.2:47556221-47556240 MS.gene65652:intron 20.0%
!! TATAAAGGAAAACTAGAAAA+TGG + chr5.2:47556220-47556239 MS.gene65652:intron 20.0%
!! TTCAAGTAACAAAATGATAT+AGG - chr5.2:47556067-47556086 None:intergenic 20.0%
!!! AAGACAAATGAATTATTTTG+AGG - chr5.2:47555208-47555227 None:intergenic 20.0%
!!! ACTCATCTTTTTTTTTTGAA+TGG - chr5.2:47555923-47555942 None:intergenic 20.0%
!!! AGACAAATGAATTATTTTGA+GGG - chr5.2:47555207-47555226 None:intergenic 20.0%
!!! TAGTAGCTATTTTTTGATAA+AGG + chr5.2:47555135-47555154 MS.gene65652:CDS 20.0%
! AGATTAAATGCAATCATGAA+TGG + chr5.2:47555840-47555859 MS.gene65652:intron 25.0%
! GATTAAGAGTGACTATTATT+AGG - chr5.2:47555279-47555298 None:intergenic 25.0%
! TAAAGGAAAACTAGAAAATG+GGG + chr5.2:47556222-47556241 MS.gene65652:intron 25.0%
! TAATGCAAAGTAGAAAACTA+GGG + chr5.2:47555976-47555995 MS.gene65652:intron 25.0%
! TTAATGCAAAGTAGAAAACT+AGG + chr5.2:47555975-47555994 MS.gene65652:intron 25.0%
!! AAACTTTGTGTATTTTGTTC+GGG + chr5.2:47555611-47555630 MS.gene65652:CDS 25.0%
!! CAAACTTTGTGTATTTTGTT+CGG + chr5.2:47555610-47555629 MS.gene65652:CDS 25.0%
!! CTACTTTGCATTAATTTTTC+TGG - chr5.2:47555969-47555988 None:intergenic 25.0%
!!! AAACTAGTGGAGTTTTTTAT+CGG - chr5.2:47555429-47555448 None:intergenic 25.0%
AAAACTAGAAAATGGGGAAA+TGG + chr5.2:47556228-47556247 MS.gene65652:intron 30.0%
AAGATGAAATCCTTGTGATA+TGG - chr5.2:47555759-47555778 None:intergenic 30.0%
ACAATAACTCAATCTCGTTA+TGG - chr5.2:47556303-47556322 None:intergenic 30.0%
AGATGAAATCCTTGTGATAT+GGG - chr5.2:47555758-47555777 None:intergenic 30.0%
ATTTCATCTTCAACTCCTTA+GGG + chr5.2:47555769-47555788 MS.gene65652:CDS 30.0%
CAATTCAATGGACAACAATA+TGG + chr5.2:47555656-47555675 MS.gene65652:CDS 30.0%
CGAACAAAATACACAAAGTT+TGG - chr5.2:47555612-47555631 None:intergenic 30.0%
TACAACAAGATCAAAACTAG+TGG - chr5.2:47555442-47555461 None:intergenic 30.0%
TGACATCATTAGCAATTCAA+TGG + chr5.2:47555644-47555663 MS.gene65652:CDS 30.0%
!! TTCATGATTTGCTACTTTGA+TGG - chr5.2:47556257-47556276 None:intergenic 30.0%
!!! AACTAGTGGAGTTTTTTATC+GGG - chr5.2:47555428-47555447 None:intergenic 30.0%
!!! GATTTTGGTTTACAAGTACA+TGG + chr5.2:47556384-47556403 MS.gene65652:CDS 30.0%
!!! GGTAACTAGTTCTAAGTTTT+TGG + chr5.2:47556111-47556130 MS.gene65652:intron 30.0%
!!! GTTTTTGCATTTCTAGCATT+TGG + chr5.2:47556038-47556057 MS.gene65652:intron 30.0%
AAAGTAGAAAACTAGGGATG+CGG + chr5.2:47555982-47556001 MS.gene65652:intron 35.0%
AACGAGATTGAGTTATTGTC+CGG + chr5.2:47556304-47556323 MS.gene65652:intron 35.0%
AACGCCTTATGATATGCTTA+GGG + chr5.2:47556467-47556486 MS.gene65652:CDS 35.0%
AAGACTTTGTTCCTTTGTCT+TGG + chr5.2:47556440-47556459 MS.gene65652:CDS 35.0%
ACTGCAACCAGTTAATATGA+TGG - chr5.2:47556012-47556031 None:intergenic 35.0%
AGAATTGCATCAAAGTCACA+TGG + chr5.2:47555555-47555574 MS.gene65652:CDS 35.0%
AGAGTGACTATTATTAGGTC+AGG - chr5.2:47555274-47555293 None:intergenic 35.0%
ATTGAAGACCCAACTTAGAT+CGG - chr5.2:47555583-47555602 None:intergenic 35.0%
CAAATCCTTATGCTCCAATA+AGG - chr5.2:47556192-47556211 None:intergenic 35.0%
CTTATTGGAGCATAAGGATT+TGG + chr5.2:47556190-47556209 MS.gene65652:intron 35.0%
CTTGATCTTTCATCCAAAAC+CGG + chr5.2:47555720-47555739 MS.gene65652:CDS 35.0%
GAGTGACTATTATTAGGTCA+GGG - chr5.2:47555273-47555292 None:intergenic 35.0%
GATTTCATCTTCAACTCCTT+AGG + chr5.2:47555768-47555787 MS.gene65652:CDS 35.0%
GCAAATCATGAATCTAGACA+AGG + chr5.2:47556265-47556284 MS.gene65652:intron 35.0%
GTAGGTTGTTGTAATGAAGA+CGG + chr5.2:47556355-47556374 MS.gene65652:intron 35.0%
GTTACTTGAAAAGTCGTATG+AGG + chr5.2:47556077-47556096 MS.gene65652:CDS 35.0%
TCGTATGAGGAAGAAGAAAT+CGG + chr5.2:47556090-47556109 MS.gene65652:CDS 35.0%
TGACTTTGATGCAATTCTCA+AGG - chr5.2:47555553-47555572 None:intergenic 35.0%
TGGTATAGAGATAACTTCGA+TGG - chr5.2:47555239-47555258 None:intergenic 35.0%
TTGATCTTTCATCCAAAACC+GGG + chr5.2:47555721-47555740 MS.gene65652:CDS 35.0%
! AACAAGAGTATATTGCAGCA+GGG - chr5.2:47555473-47555492 None:intergenic 35.0%
! GGACTTTTATATCTTCACAC+CGG + chr5.2:47556499-47556518 MS.gene65652:CDS 35.0%
! GGATTTGGAAAGGTGTATAA+AGG + chr5.2:47556205-47556224 MS.gene65652:intron 35.0%
! TTTGGAACACTTTACTTTGC+AGG + chr5.2:47556129-47556148 MS.gene65652:intron 35.0%
ACATGGATCCGATCTAAGTT+GGG + chr5.2:47555572-47555591 MS.gene65652:CDS 40.0%
ACGCCTTATGATATGCTTAG+GGG + chr5.2:47556468-47556487 MS.gene65652:CDS 40.0%
ACTTGATGTCCCATATCACA+AGG + chr5.2:47555746-47555765 MS.gene65652:CDS 40.0%
CAACGCCTTATGATATGCTT+AGG + chr5.2:47556466-47556485 MS.gene65652:CDS 40.0%
CACTCTCTACACAGTTTCAA+GGG - chr5.2:47555335-47555354 None:intergenic 40.0%
GTTTACAAGTACATGGCCAA+TGG + chr5.2:47556391-47556410 MS.gene65652:CDS 40.0%
TCACTCTCTACACAGTTTCA+AGG - chr5.2:47555336-47555355 None:intergenic 40.0%
TGATCTTTCATCCAAAACCG+GGG + chr5.2:47555722-47555741 MS.gene65652:CDS 40.0%
TGATGCAATTCTCAAGGTTC+TGG - chr5.2:47555547-47555566 None:intergenic 40.0%
TGGAGCATAAGGATTTGGAA+AGG + chr5.2:47556195-47556214 MS.gene65652:intron 40.0%
TGTGTAGAGAGTGAATGTCA+GGG + chr5.2:47555342-47555361 MS.gene65652:intron 40.0%
TTTGATCCTGAGCTCCTTAT+TGG + chr5.2:47556175-47556194 MS.gene65652:intron 40.0%
! CAACAAGAGTATATTGCAGC+AGG - chr5.2:47555474-47555493 None:intergenic 40.0%
! CACTTTCAAAGTGTTCCCTA+AGG - chr5.2:47555787-47555806 None:intergenic 40.0%
!! TGAAGACGGAGAACTGATTT+TGG + chr5.2:47556369-47556388 MS.gene65652:CDS 40.0%
!!! TAGTGGAGTTTTTTATCGGG+TGG - chr5.2:47555425-47555444 None:intergenic 40.0%
AACAACCTACAAGAGAGACG+AGG - chr5.2:47556345-47556364 None:intergenic 45.0%
AGGTCAGGGAATAGTTCATC+TGG - chr5.2:47555259-47555278 None:intergenic 45.0%
ATATGCTTAGGGGCAGCAAA+GGG + chr5.2:47556478-47556497 MS.gene65652:CDS 45.0%
ATGACTGCTGAGAGATCCAT+TGG - chr5.2:47556410-47556429 None:intergenic 45.0%
CACATGGATCCGATCTAAGT+TGG + chr5.2:47555571-47555590 MS.gene65652:CDS 45.0%
CGGTGGACCATCATATTAAC+TGG + chr5.2:47556002-47556021 MS.gene65652:intron 45.0%
CTGCCCCTAAGCATATCATA+AGG - chr5.2:47556474-47556493 None:intergenic 45.0%
CTGGCTGTAGCATAAACCAA+TGG - chr5.2:47555528-47555547 None:intergenic 45.0%
CTGTGTAGAGAGTGAATGTC+AGG + chr5.2:47555341-47555360 MS.gene65652:intron 45.0%
GAGCTCCTTATTGGAGCATA+AGG + chr5.2:47556184-47556203 MS.gene65652:intron 45.0%
GAGGTTTGAATGACGAAGAC+CGG - chr5.2:47556326-47556345 None:intergenic 45.0%
GATCTTTCATCCAAAACCGG+GGG + chr5.2:47555723-47555742 MS.gene65652:CDS 45.0%
GCTGTAGCATAAACCAATGG+TGG - chr5.2:47555525-47555544 None:intergenic 45.0%
TATGCTCCAATAAGGAGCTC+AGG - chr5.2:47556184-47556203 None:intergenic 45.0%
TCAAACCTCGTCTCTCTTGT+AGG + chr5.2:47556337-47556356 MS.gene65652:intron 45.0%
TCAACTTTGAGTGACACGCT+TGG + chr5.2:47555366-47555385 MS.gene65652:CDS 45.0%
TCTCTCAGCAGTCATCTCTA+TGG + chr5.2:47556415-47556434 MS.gene65652:CDS 45.0%
!!! TGGAGTTTTTTATCGGGTGG+TGG - chr5.2:47555422-47555441 None:intergenic 45.0%
ATATGGGACATCAAGTCCCC+CGG - chr5.2:47555742-47555761 None:intergenic 50.0%
CTTTGAGTGACACGCTTGGA+AGG + chr5.2:47555370-47555389 MS.gene65652:CDS 50.0%
GATATGCTTAGGGGCAGCAA+AGG + chr5.2:47556477-47556496 MS.gene65652:CDS 50.0%
GTAGAAAACTAGGGATGCGG+TGG + chr5.2:47555985-47556004 MS.gene65652:intron 50.0%
TGACACGCTTGGAAGGACAT+GGG + chr5.2:47555377-47555396 MS.gene65652:CDS 50.0%
! AGGCGTTGCTTCCAAGACAA+AGG - chr5.2:47556454-47556473 None:intergenic 50.0%
! GCATAAACCAATGGTGGAGC+TGG - chr5.2:47555519-47555538 None:intergenic 50.0%
! TAAACCAATGGTGGAGCTGG+TGG - chr5.2:47555516-47555535 None:intergenic 50.0%
GTGACACGCTTGGAAGGACA+TGG + chr5.2:47555376-47555395 MS.gene65652:CDS 55.0%
! GACATCAAGTCCCCCGGTTT+TGG - chr5.2:47555736-47555755 None:intergenic 55.0%
AGCTCCACCAGCTCCACCAT+TGG + chr5.2:47555509-47555528 MS.gene65652:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 47555127 47556528 47555127 ID=MS.gene65652
chr5.2 mRNA 47555127 47556528 47555127 ID=MS.gene65652.t1;Parent=MS.gene65652
chr5.2 exon 47555127 47555243 47555127 ID=MS.gene65652.t1.exon1;Parent=MS.gene65652.t1
chr5.2 CDS 47555127 47555243 47555127 ID=cds.MS.gene65652.t1;Parent=MS.gene65652.t1
chr5.2 exon 47555363 47555803 47555363 ID=MS.gene65652.t1.exon2;Parent=MS.gene65652.t1
chr5.2 CDS 47555363 47555803 47555363 ID=cds.MS.gene65652.t1;Parent=MS.gene65652.t1
chr5.2 exon 47556054 47556111 47556054 ID=MS.gene65652.t1.exon3;Parent=MS.gene65652.t1
chr5.2 CDS 47556054 47556111 47556054 ID=cds.MS.gene65652.t1;Parent=MS.gene65652.t1
chr5.2 exon 47556359 47556528 47556359 ID=MS.gene65652.t1.exon4;Parent=MS.gene65652.t1
chr5.2 CDS 47556359 47556528 47556359 ID=cds.MS.gene65652.t1;Parent=MS.gene65652.t1
Gene Sequence

>MS.gene65652

ATGAATTATAGTAGCTATTTTTTGATAAAGGAGTATGCAATCAACATTACAACAGATGAGTTATCACTTACTTTCATCCCTCAAAATAATTCATTTGTCTTTGTTAATGCCATCGAAGTTATCTCTATACCAGATGAACTATTCCCTGACCTAATAATAGTCACTCTTAATCCATCAAGTTTTTATGAACAATTCTCAAAATCTTCCCTTGAAACTGTGTAGAGAGTGAATGTCAGGGTTCAACTTTGAGTGACACGCTTGGAAGGACATGGGAGAATGATGAATATTACTTCCACCACCCGATAAAAAACTCCACTAGTTTTGATCTTGTTGTATCTACTAACCCTGCTGCAATATACTCTTGTTGCGCTACGCTTATTACAGCTCCACCAGCTCCACCATTGGTTTATGCTACAGCCAGAACCTTGAGAATTGCATCAAAGTCACATGGATCCGATCTAAGTTGGGTCTTCAATGTAGATCCAAACTTTGTGTATTTTGTTCGGGTTCATTTATGTGACATCATTAGCAATTCAATGGACAACAATATGGTTTTCAATTTATTTATAAATGAAGCTTATGTTTTTAGAAGTCTTGATCTTTCATCCAAAACCGGGGGACTTGATGTCCCATATCACAAGGATTTCATCTTCAACTCCTTAGGGAACACTTTGAAAGTGAGTGTGAGTGTTAATCGTGATACAATTAAAGAAAGATTAAATGCAATCATGAATGGTATCGAGATACTAAAGATTAGCAACAACGTAGATAGATTAGATGAGTTTTCTTTTAGCCATTCAAAAAAAAAAGATGAGTTTTCTTCTAGTGTTTTGCATTCACCAGAAAAATTAATGCAAAGTAGAAAACTAGGGATGCGGTGGACCATCATATTAACTGGTTGCAGTGTTTGTGTTTTTGCATTTCTAGCATTTGGCAGCCTATATCATTTTGTTACTTGAAAAGTCGTATGAGGAAGAAGAAATCGGTAACTAGTTCTAAGTTTTTGGAACACTTTACTTTGCAGGAGATGCAACAAGCAACAAATAATTTTGATCCTGAGCTCCTTATTGGAGCATAAGGATTTGGAAAGGTGTATAAAGGAAAACTAGAAAATGGGGAAATGGTAGCCATCAAAGTAGCAAATCATGAATCTAGACAAGGTCTCAGTGAATTCCATAACGAGATTGAGTTATTGTCCGGTCTTCGTCATTCAAACCTCGTCTCTCTTGTAGGTTGTTGTAATGAAGACGGAGAACTGATTTTGGTTTACAAGTACATGGCCAATGGATCTCTCAGCAGTCATCTCTATGGAAAAGACTTTGTTCCTTTGTCTTGGAAGCAACGCCTTATGATATGCTTAGGGGCAGCAAAGGGACTTTTATATCTTCACACCGGAGCTTAA
Protein sequence

>MS.gene65652.t1

MNYSSYFLIKEYAINITTDELSLTFIPQNNSFVFVNAIEGSTLSDTLGRTWENDEYYFHHPIKNSTSFDLVVSTNPAAIYSCCATLITAPPAPPLVYATARTLRIASKSHGSDLSWVFNVDPNFVYFVRVHLCDIISNSMDNNMVFNLFINEAYVFRSLDLSSKTGGLDVPYHKDFIFNSLGNTLKHLAAYIILLLEKSYEEEEIGCCNEDGELILVYKYMANGSLSSHLYGKDFVPLSWKQRLMICLGAAKGLLYLHTGA