Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65911.t1 | RHN45637.1 | 82.7 | 81 | 13 | 1 | 2 | 82 | 186 | 265 | 5.70E-31 | 143.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65911.t1 | G7KVV4 | 82.7 | 81 | 13 | 1 | 2 | 82 | 213 | 292 | 4.1e-31 | 143.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene65911.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65911.t1 | MTR_7g050560 | 82.716 | 81 | 13 | 1 | 2 | 82 | 213 | 292 | 9.85e-44 | 144 |
| MS.gene65911.t1 | MTR_7g050580 | 76.543 | 81 | 19 | 0 | 2 | 82 | 212 | 292 | 5.92e-42 | 139 |
| MS.gene65911.t1 | MTR_7g050590 | 74.390 | 82 | 20 | 1 | 2 | 82 | 201 | 282 | 1.13e-38 | 130 |
| MS.gene65911.t1 | MTR_3g098790 | 65.854 | 82 | 28 | 0 | 1 | 82 | 220 | 301 | 7.32e-34 | 119 |
| MS.gene65911.t1 | MTR_7g050710 | 60.714 | 84 | 31 | 1 | 1 | 82 | 226 | 309 | 7.61e-31 | 110 |
| MS.gene65911.t1 | MTR_7g050720 | 57.647 | 85 | 32 | 4 | 2 | 82 | 233 | 317 | 8.63e-24 | 92.0 |
| MS.gene65911.t1 | MTR_1g035460 | 43.038 | 79 | 35 | 2 | 2 | 80 | 219 | 287 | 2.48e-13 | 63.9 |
| MS.gene65911.t1 | MTR_1g108780 | 42.466 | 73 | 33 | 2 | 9 | 80 | 226 | 290 | 1.55e-12 | 62.0 |
| MS.gene65911.t1 | MTR_8g103940 | 47.945 | 73 | 29 | 3 | 9 | 80 | 267 | 331 | 2.39e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTTGCAGGTGTTAAATGA+AGG | + | 50847:6328-6347 | MS.gene65911:CDS | 30.0% | |
| TGGTAATAGACACTTTGATT+TGG | + | 50847:6285-6304 | MS.gene65911:CDS | 30.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | + | 50847:6335-6354 | MS.gene65911:CDS | 35.0% | |
| GAGGACAATAACTTGAAAGT+TGG | + | 50847:6433-6452 | MS.gene65911:CDS | 35.0% | |
| !! | TTTGATTTGGATCAGAAGAG+AGG | + | 50847:6298-6317 | MS.gene65911:CDS | 35.0% |
| ATCCTGATTAGGAACTTTGC+AGG | - | 50847:6361-6380 | MS.gene65911:intergenic | 40.0% | |
| GAGAGGAGATGTTTATTTGC+AGG | + | 50847:6315-6334 | MS.gene65911:CDS | 40.0% | |
| TCTAACATGTGGTCCTGTAA+GGG | - | 50847:6386-6405 | MS.gene65911:intergenic | 40.0% | |
| ATGTTAGACTCTCAGGTGGA+TGG | + | 50847:6398-6417 | MS.gene65911:CDS | 45.0% | |
| CTAATCAGGATGACCCTTAC+AGG | + | 50847:6370-6389 | MS.gene65911:CDS | 45.0% | |
| CTGTAAGGGTCATCCTGATT+AGG | - | 50847:6372-6391 | MS.gene65911:intergenic | 45.0% | |
| GTCTAACATGTGGTCCTGTA+AGG | - | 50847:6387-6406 | MS.gene65911:intergenic | 45.0% | |
| CCACATGTTAGACTCTCAGG+TGG | + | 50847:6394-6413 | MS.gene65911:CDS | 50.0% | |
| CCACCTGAGAGTCTAACATG+TGG | - | 50847:6397-6416 | MS.gene65911:intergenic | 50.0% | |
| GGACCACATGTTAGACTCTC+AGG | + | 50847:6391-6410 | MS.gene65911:CDS | 50.0% | |
| GGCCTGCAAAGTTCCTAATC+AGG | + | 50847:6356-6375 | MS.gene65911:CDS | 50.0% | |
| TAGACTCTCAGGTGGATGGA+AGG | + | 50847:6402-6421 | MS.gene65911:CDS | 50.0% | |
| !! | TGGATGGAAGGCATTTGCAG+AGG | + | 50847:6414-6433 | MS.gene65911:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50847 | gene | 6280 | 6558 | 6280 | ID=MS.gene65911 |
| 50847 | mRNA | 6280 | 6558 | 6280 | ID=MS.gene65911.t1;Parent=MS.gene65911 |
| 50847 | exon | 6280 | 6558 | 6280 | ID=MS.gene65911.t1.exon1;Parent=MS.gene65911.t1 |
| 50847 | CDS | 6280 | 6558 | 6280 | ID=cds.MS.gene65911.t1;Parent=MS.gene65911.t1 |
| Gene Sequence |
| Protein sequence |
