AlfalfaGEDB | MS.gene65988

M. sativa cultivar XinJiangDaYe / MS.gene65988

KEGG: K13422
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29 PF14215.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65988.t1	XP_003628820.2	92.4	649	48	1	1	649	1	648	7.00E-301	1042.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65988.t1	A0A3Q7HRZ6	52.6	699	251	16	2	645	14	687	2.8e-157	557.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65988.t1	G7LHR2	92.4	649	48	1	1	649	1	648	5.0e-301	1042.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene65988.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65988.t1	MTR_8g067280	96.148	649	24	1	1	649	1	648	0.0	1197
MS.gene65988.t1	MTR_5g030430	59.885	698	205	19	3	647	2	677	0.0	712
MS.gene65988.t1	MTR_5g030420	61.905	210	73	4	34	238	5	212	1.16e-84	265
MS.gene65988.t1	MTR_5g030420	61.905	210	73	4	34	238	5	212	1.49e-84	266
MS.gene65988.t1	MTR_8g024790	47.264	201	95	3	445	644	273	463	3.33e-52	187
MS.gene65988.t1	MTR_8g024790	30.244	205	106	5	43	243	19	190	3.94e-24	106
MS.gene65988.t1	MTR_8g024770	49.223	193	90	3	445	636	261	446	3.16e-51	184
MS.gene65988.t1	MTR_8g024770	28.177	181	87	5	67	243	36	177	7.20e-18	87.0
MS.gene65988.t1	MTR_8g027495	44.444	189	85	5	453	640	448	617	6.24e-37	146
MS.gene65988.t1	MTR_8g027495	34.694	196	110	8	52	234	51	241	2.49e-21	99.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65988.t1	AT1G32640	50.898	668	247	19	3	644	11	623	0.0	575
MS.gene65988.t1	AT4G17880	47.344	659	240	18	3	646	22	588	0.0	532
MS.gene65988.t1	AT5G46760	47.239	652	255	16	6	645	16	590	8.62e-169	495
MS.gene65988.t1	AT5G46830	56.545	191	68	4	454	643	334	510	1.19e-55	197
MS.gene65988.t1	AT5G46830	32.225	391	166	10	6	377	5	315	8.31e-43	162
MS.gene65988.t1	AT1G01260	38.202	267	112	6	374	640	362	575	2.68e-40	156
MS.gene65988.t1	AT1G01260	38.144	194	106	8	52	234	46	236	2.78e-25	111
MS.gene65988.t1	AT1G01260	38.202	267	112	6	374	640	362	575	2.68e-40	156
MS.gene65988.t1	AT1G01260	38.144	194	106	8	52	234	46	236	2.78e-25	111
MS.gene65988.t1	AT1G01260	38.202	267	112	6	374	640	362	575	2.68e-40	156

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Find 166 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATATGAATAACATTT+GGG	0.401501	8.1:-37376296	None:intergenic
AAATCAACGTATTAAGCTCA+CGG	0.614664	8.1:+37375930	None:intergenic
AAATCAAGCAGCCGAGGTGG+AGG	0.534413	8.1:-37375056	MS.gene65988:CDS
AACATAGACTGTTGTGATGG+AGG	0.624047	8.1:+37376166	None:intergenic
AACCCCGGTGTGGATCACCG+GGG	0.772910	8.1:-37375777	MS.gene65988:CDS
AACGGCCACGAACCTGTTTC+AGG	0.325408	8.1:+37375575	None:intergenic
AAGAAAAGTTCTTATATCAC+CGG	0.523073	8.1:-37375221	MS.gene65988:CDS
AAGATGTCACCGACACTGAG+TGG	0.679436	8.1:-37375871	MS.gene65988:CDS
AAGCCAGAAATTAGAGAGGT+CGG	0.590490	8.1:+37376234	None:intergenic
AATATGAAATCAAGCAGCCG+AGG	0.686490	8.1:-37375062	MS.gene65988:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCAACGTATTAAGCTCA+CGG	+	chr8.1:37374724-37374743	None:intergenic	30.0%
AATTGAAATCGAAGCTACAA+GGG	-	chr8.1:37375881-37375900	MS.gene65988:CDS	30.0%
AATTTCAACAATCCTGAAAC+AGG	-	chr8.1:37375064-37375083	MS.gene65988:CDS	30.0%
AGAAAAGAAAGTTCCATTTC+CGG	+	chr8.1:37375452-37375471	None:intergenic	30.0%
ATTTCTGCAAATGCAACAAT+CGG	-	chr8.1:37375226-37375245	MS.gene65988:CDS	30.0%
CTCTTCTAAGTTAATTGACT+TGG	-	chr8.1:37376041-37376060	MS.gene65988:CDS	30.0%
GGATATTGAAGTGAAGATTA+TGG	-	chr8.1:37376062-37376081	MS.gene65988:CDS	30.0%
GTTGAACCATTTGTTTCAAA+CGG	+	chr8.1:37375262-37375281	None:intergenic	30.0%
TCTCTTCTTTCTTCCAAAAT+TGG	-	chr8.1:37376270-37376289	MS.gene65988:CDS	30.0%
TTACTAATTCTGTCTGTGAA+CGG	-	chr8.1:37374906-37374925	MS.gene65988:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	37374362	37376311	37374362	ID=MS.gene65988
chr8.1	mRNA	37374362	37376311	37374362	ID=MS.gene65988.t1;Parent=MS.gene65988
chr8.1	exon	37374362	37376311	37374362	ID=MS.gene65988.t1.exon1;Parent=MS.gene65988.t1
chr8.1	CDS	37374362	37376311	37374362	ID=cds.MS.gene65988.t1;Parent=MS.gene65988.t1

Gene Sequence

>MS.gene65988

ATGAATAACATTTGGGACGACAACTCCTCCGTCATGGAAGCTTTCATGACCACCTCCGACCTCTCTAATTTCTGGCTTCCAACACCACATTCCGCAACTTCAACAACTGCACCACCAGTGCCTCCTCCATCACAACAGTCTATGTTCAATCAAGAGACTCTCCAACACCGTCTTCAAGCTCTGATTGAAGGCGCGAAAGAAAGCTGGACCTACGCGATTTTCTGGCAATCCTCTTATGATTACACCATGGCTACACCGCTGCTCGGCTGGGGTGATGGATATTACAAAGGGGAAGATGACAAAGGAAAACTGAAACGAGTCACGCCTCCAGAAGAACAGGCTCATCGTAGAAAGATTCTCCGTGAGCTTAATACGTTGATTTCAGGTGGTTCGTCTGTCTCAGATGATGCCGTTGAGGAAGATGTCACCGACACTGAGTGGTTTTTTCTCACGTCGATGACTCAATCCTTTGTCAACGGCACCGGATCATTGAGCCAGGCTTATTTCAATTCAACCCCGGTGTGGATCACCGGGGCTGAAAGGCTTACTAATTCTGTCTGTGAACGGGCCCGTGAAGCACGGGTCCATGGATTTCAGACCTTGGTATGTATACCAACGTCTTCAAGCGGGGTGGTTGAGCTTGCTTCTACGGAAATGATTCCATACAACGCTGATCTAATGGAGAAGATTCGTGTTTTGTTTAATTTCAACAATCCTGAAACAGGTTCGTGGCCGTTGAATTCCATTACAACGAGCGAGAACGATCCTTCATCTGTCTGGCTCAATGATCTCTCCGCCTCTGCAGCTATGGAAATTCGAGATACCACCGTTAACACCGCCACCGTACCTGCTATGAACGCATCCATTTCTGCAAATGCAACAATCGGTAAATCATTACCGTTTGAAACAAATGGTTCAACCAGTACTCTAACGGAAACACCAACTGCAGTTAACTTTGCTCAGCGTCAGAATCAGAACAACCAGAACCAGAGCTTTTTTCTGAAGGAACTGAATTTTTCTGGTTCAATGAAACCAGAATCCGGGGAGATTCTGAGTTTCGGTGAAAGTAAGAAAAGTTCTTATATCACCGGAAATGGAACTTTCTTTTCTGGTCAGTCACAGTTCGTTGCCAGTGAAGAAAACAGAAAAAGAAAGTCTCCTGTATCCAGAAGCAGCATTGACGATGGAATGCTTTCGTTTACTTCCGGTGTAGTCTTACCGTCTTCAAATATGAAATCAAGCAGCCGAGGTGGAGGCGGAGATTCTGATCATTCAGATCTAGATGTTTCTGCTGTGAAGGAAGGAGAGAGTAGCAGGGTGGTTGAGCCAGGGAAGCGACCGAAGAAACGAGGTAGAAAACCGGCTAATGGAAGAGAAGAACCGTTGAATCATGTAGAAGCAGAGAGACAGAGAAGAGAAAAGCTTAACCAGAAATTCTACGCTCTACGCGCTGTGGTTCCAAATGTTTCCAAAATGGACAAAGCATCGCTGCTAGGCGATGCTATTTCTTATATAAACGAATTGAAATCGAAGCTACAAGGGCTGGAATCGTCTAAAGGTGAATTGGAGAAACAATTGGGTGCAACAAAGAAGGAACTGGAACTTGTTACTAGTAAAAATCAAAATCAAAATCCAATTCCATTAGATAAAGAAAAGGAGAAAACAACAAGCTCAACAAGCTCTTCTAAGTTAATTGACTTGGATATTGAAGTGAAGATTATGGGTTGGGATGCTATGATTAGGATTCAATGCAGCAAGAAGAATCATCCTGCGGCGAAGTTGATGGCGGCGTTGAAAGACTTAGACTTGGATGTTAATCATGCTAGTGTGTCTGTTGTGAATGATTTGATGATTCAACAAGCTTCTGTTAACATGGGAAGTCGATTTTACACACAAGAACAACTTTTGTCTCTTCTTTCTTCCAAAATTGGTGATGCACAAGGAGATTGA

Protein sequence

>MS.gene65988.t1

MNNIWDDNSSVMEAFMTTSDLSNFWLPTPHSATSTTAPPVPPPSQQSMFNQETLQHRLQALIEGAKESWTYAIFWQSSYDYTMATPLLGWGDGYYKGEDDKGKLKRVTPPEEQAHRRKILRELNTLISGGSSVSDDAVEEDVTDTEWFFLTSMTQSFVNGTGSLSQAYFNSTPVWITGAERLTNSVCERAREARVHGFQTLVCIPTSSSGVVELASTEMIPYNADLMEKIRVLFNFNNPETGSWPLNSITTSENDPSSVWLNDLSASAAMEIRDTTVNTATVPAMNASISANATIGKSLPFETNGSTSTLTETPTAVNFAQRQNQNNQNQSFFLKELNFSGSMKPESGEILSFGESKKSSYITGNGTFFSGQSQFVASEENRKRKSPVSRSSIDDGMLSFTSGVVLPSSNMKSSSRGGGGDSDHSDLDVSAVKEGESSRVVEPGKRPKKRGRKPANGREEPLNHVEAERQRREKLNQKFYALRAVVPNVSKMDKASLLGDAISYINELKSKLQGLESSKGELEKQLGATKKELELVTSKNQNQNPIPLDKEKEKTTSSTSSSKLIDLDIEVKIMGWDAMIRIQCSKKNHPAAKLMAALKDLDLDVNHASVSVVNDLMIQQASVNMGSRFYTQEQLLSLLSSKIGDAQGD