Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66029.t1 | PNX89523.1 | 40.8 | 338 | 184 | 5 | 17 | 352 | 11 | 334 | 6.70E-11 | 78.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66029.t1 | A0A2K3MFE8 | 40.8 | 338 | 184 | 5 | 17 | 352 | 11 | 334 | 4.8e-11 | 78.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene66029.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 161 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTTAAAAAAGAACAC+AGG | + | 37911:38530-38549 | MS.gene66029:intron | 15.0% |
| !!! | AAATGATAACAATTTAAAAC+AGG | - | 37911:39472-39491 | None:intergenic | 15.0% |
| !!! | AATTACTGTTATATAGTTTT+TGG | + | 37911:39431-39450 | MS.gene66029:intron | 15.0% |
| ! | AAATCAAAGAAATTATGTGG+AGG | - | 37911:39178-39197 | None:intergenic | 25.0% |
| ! | AGTACACAAAATCTTAGATA+CGG | - | 37911:38190-38209 | None:intergenic | 25.0% |
| ! | GCAAAATCAAAGAAATTATG+TGG | - | 37911:39181-39200 | None:intergenic | 25.0% |
| !!! | CATTTGTTGTTACATGTTTA+AGG | + | 37911:38077-38096 | MS.gene66029:intron | 25.0% |
| AAATATATTGCAGAGTGACA+AGG | - | 37911:38273-38292 | None:intergenic | 30.0% | |
| AGTACACATTATCGTAGATA+CGG | - | 37911:38377-38396 | None:intergenic | 30.0% | |
| ATTACAGAGTGACAAAAAAC+TGG | - | 37911:38421-38440 | None:intergenic | 30.0% | |
| TATAATTCATACTCCTCCAA+GGG | - | 37911:38950-38969 | None:intergenic | 30.0% | |
| TTACAGAGTGACAAAAAACT+GGG | - | 37911:38420-38439 | None:intergenic | 30.0% | |
| TTGTTACTAAACTTTGAGTG+TGG | + | 37911:37974-37993 | MS.gene66029:intron | 30.0% | |
| AAAATCTACCGGATACCAAA+AGG | - | 37911:38048-38067 | None:intergenic | 35.0% | |
| AAATCTACCGGATACCAAAA+GGG | - | 37911:38047-38066 | None:intergenic | 35.0% | |
| AACATCAATGCCAAGATTGA+TGG | - | 37911:39014-39033 | None:intergenic | 35.0% | |
| AAGTGGTTGTTGAAGTATGA+TGG | - | 37911:38650-38669 | None:intergenic | 35.0% | |
| ACCCATATGTTATGTATGCT+AGG | + | 37911:39720-39739 | MS.gene66029:CDS | 35.0% | |
| ACTCCTTAGCCTATAGAAAA+AGG | - | 37911:38759-38778 | None:intergenic | 35.0% | |
| ATACTGCGTCTAAGTTACAA+CGG | + | 37911:38812-38831 | MS.gene66029:CDS | 35.0% | |
| ATCTTCATCGTTGATATCCA+TGG | - | 37911:39209-39228 | None:intergenic | 35.0% | |
| ATTACAGAGTGACAACAAAC+AGG | - | 37911:38234-38253 | None:intergenic | 35.0% | |
| CCCATATGTTATGTATGCTA+GGG | + | 37911:39721-39740 | MS.gene66029:CDS | 35.0% | |
| CCCTAGCATACATAACATAT+GGG | - | 37911:39724-39743 | None:intergenic | 35.0% | |
| GGTAACAATGGTGAAAACAT+GGG | + | 37911:39289-39308 | MS.gene66029:CDS | 35.0% | |
| GTATAATTCATACTCCTCCA+AGG | - | 37911:38951-38970 | None:intergenic | 35.0% | |
| TCAAAGAAATTATGTGGAGG+CGG | - | 37911:39175-39194 | None:intergenic | 35.0% | |
| TCATACTTCAACAACCACTT+TGG | + | 37911:38650-38669 | MS.gene66029:CDS | 35.0% | |
| TCCCTAGCATACATAACATA+TGG | - | 37911:39725-39744 | None:intergenic | 35.0% | |
| TTCTTGTGTGTGCATAATAG+TGG | - | 37911:38166-38185 | None:intergenic | 35.0% | |
| TTGATTGGCATCATGAACTT+CGG | - | 37911:39122-39141 | None:intergenic | 35.0% | |
| TTTACAGAGTGACATCAAAC+TGG | - | 37911:38459-38478 | None:intergenic | 35.0% | |
| ! | GAAGTTGTCACTTGTAATGT+AGG | + | 37911:38610-38629 | MS.gene66029:intron | 35.0% |
| ! | TTATTTTCCTGTCTCCCTTT+TGG | + | 37911:38030-38049 | MS.gene66029:intron | 35.0% |
| AACCAACAACAAGGCAAACA+TGG | - | 37911:38299-38318 | None:intergenic | 40.0% | |
| AACGATGAAGATGTGGAAGA+TGG | + | 37911:39217-39236 | MS.gene66029:CDS | 40.0% | |
| ACTCGTGCAACAAATCTTCT+TGG | - | 37911:39509-39528 | None:intergenic | 40.0% | |
| ATGTTTGCCTTCTTGTTGGT+TGG | + | 37911:38109-38128 | MS.gene66029:intron | 40.0% | |
| GACGATGATTCCATCAATCT+TGG | + | 37911:39001-39020 | MS.gene66029:CDS | 40.0% | |
| GACTATGTTTGCCTTCTTGT+TGG | + | 37911:38105-38124 | MS.gene66029:intron | 40.0% | |
| GCAGCATCAAAGAAGTTATG+CGG | - | 37911:39256-39275 | None:intergenic | 40.0% | |
| GGATATCAACGATGAAGATG+TGG | + | 37911:39210-39229 | MS.gene66029:CDS | 40.0% | |
| GGGTAACAATGGTGAAAACA+TGG | + | 37911:39288-39307 | MS.gene66029:CDS | 40.0% | |
| GTCACTTGTAATGTAGGATG+AGG | + | 37911:38616-38635 | MS.gene66029:intron | 40.0% | |
| GTGCAACAAATCTTCTTGGT+AGG | - | 37911:39505-39524 | None:intergenic | 40.0% | |
| GTGGGAATGTGGCTAATAAT+GGG | + | 37911:39317-39336 | MS.gene66029:CDS | 40.0% | |
| TAAGACACACTTACCTTGTC+TGG | - | 37911:37940-37959 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAACA+AGG | - | 37911:38308-38327 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAAGA+AGG | - | 37911:38119-38138 | None:intergenic | 40.0% | |
| TGAAGCCATGCAAAATCTAC+CGG | - | 37911:38059-38078 | None:intergenic | 40.0% | |
| TGATTGGCATCATGAACTTC+GGG | - | 37911:39121-39140 | None:intergenic | 40.0% | |
| TGTCAGACTTGCAGAATACA+TGG | + | 37911:39357-39376 | MS.gene66029:CDS | 40.0% | |
| TTATGTATGCTAGGGAAGCT+CGG | + | 37911:39729-39748 | MS.gene66029:CDS | 40.0% | |
| ! | GGACCTTTTTCTATAGGCTA+AGG | + | 37911:38753-38772 | MS.gene66029:CDS | 40.0% |
| ! | GGTGTTTTCATAGGAAGTGA+GGG | + | 37911:38725-38744 | MS.gene66029:CDS | 40.0% |
| ! | GTGTTTTCATAGGAAGTGAG+GGG | + | 37911:38726-38745 | MS.gene66029:CDS | 40.0% |
| ! | GTTTTCACCATTGTTACCCA+TGG | - | 37911:39287-39306 | None:intergenic | 40.0% |
| ! | TGGTGTTTTCATAGGAAGTG+AGG | + | 37911:38724-38743 | MS.gene66029:CDS | 40.0% |
| !! | AATTATGTGGAGGCGGATTT+TGG | - | 37911:39168-39187 | None:intergenic | 40.0% |
| !! | AGAGATTTGTTACGGAGAAC+CGG | + | 37911:39654-39673 | MS.gene66029:CDS | 40.0% |
| !! | AGATTTGTTGCACGAGTGTT+TGG | + | 37911:39512-39531 | MS.gene66029:CDS | 40.0% |
| !! | ATGTTTGCCTTGTTGTTGGT+TGG | + | 37911:38298-38317 | MS.gene66029:intron | 40.0% |
| !! | ATTATGTGGAGGCGGATTTT+GGG | - | 37911:39167-39186 | None:intergenic | 40.0% |
| !! | GATTTGTTGCACGAGTGTTT+GGG | + | 37911:39513-39532 | MS.gene66029:CDS | 40.0% |
| !!! | TCGAGGTTTGGTGTTTTCAT+AGG | + | 37911:38716-38735 | MS.gene66029:CDS | 40.0% |
| AATGGTGAAAACATGGGTGC+AGG | + | 37911:39295-39314 | MS.gene66029:CDS | 45.0% | |
| AGACGCAGTATGAGACCTAA+CGG | - | 37911:38803-38822 | None:intergenic | 45.0% | |
| ATCGTCGTCGTTGTCTTCAT+CGG | - | 37911:38987-39006 | None:intergenic | 45.0% | |
| ATGAACTACGACACAGAGTC+TGG | + | 37911:38675-38694 | MS.gene66029:CDS | 45.0% | |
| CACAACAATCGATCCAGACA+AGG | + | 37911:37924-37943 | MS.gene66029:CDS | 45.0% | |
| CATGGCATCTGTAAGTAGGA+CGG | - | 37911:39555-39574 | None:intergenic | 45.0% | |
| CATGGTGAAGACTAGCAATC+TGG | + | 37911:39375-39394 | MS.gene66029:CDS | 45.0% | |
| CGTCTAAGTTACAACGGACA+GGG | + | 37911:38818-38837 | MS.gene66029:CDS | 45.0% | |
| CTATAGGCTAAGGAGTTTGC+TGG | + | 37911:38763-38782 | MS.gene66029:CDS | 45.0% | |
| GAACTTCCTGAATGTAAGCG+CGG | - | 37911:37902-37921 | None:intergenic | 45.0% | |
| GCATCAAAGAAGTTATGCGG+AGG | - | 37911:39253-39272 | None:intergenic | 45.0% | |
| GGCATTGTGTGGTTGTTGAT+TGG | - | 37911:39137-39156 | None:intergenic | 45.0% | |
| GGTGGGAATGTGGCTAATAA+TGG | + | 37911:39316-39335 | MS.gene66029:CDS | 45.0% | |
| GTCCTACTTACAGATGCCAT+GGG | + | 37911:39554-39573 | MS.gene66029:CDS | 45.0% | |
| GTCGTAGTTCATCACCAAAG+TGG | - | 37911:38667-38686 | None:intergenic | 45.0% | |
| GTCTAAGTTACAACGGACAG+GGG | + | 37911:38819-38838 | MS.gene66029:CDS | 45.0% | |
| GTCTGGTTAGAAGACACAAG+AGG | + | 37911:38692-38711 | MS.gene66029:CDS | 45.0% | |
| GTGAAGACTAGCAATCTGGT+AGG | + | 37911:39379-39398 | MS.gene66029:intron | 45.0% | |
| TAGAAGACACAAGAGGTTCG+AGG | + | 37911:38699-38718 | MS.gene66029:CDS | 45.0% | |
| ! | GACCATGTTTGCCTTGTTGT+TGG | + | 37911:38294-38313 | MS.gene66029:intron | 45.0% |
| ! | GGTATCCGGTAGATTTTGCA+TGG | + | 37911:38051-38070 | MS.gene66029:intron | 45.0% |
| ! | TGTGGCTAATAATGGGAGAG+TGG | + | 37911:39324-39343 | MS.gene66029:CDS | 45.0% |
| !! | CGGGTGGAAGAGATTTGTTA+CGG | + | 37911:39646-39665 | MS.gene66029:CDS | 45.0% |
| !! | CTTTGATTTTGCTGCTGCCA+TGG | + | 37911:39189-39208 | MS.gene66029:CDS | 45.0% |
| !! | TGGCAGTTGGGAAAAGTGTT+AGG | + | 37911:39678-39697 | MS.gene66029:CDS | 45.0% |
| ACTCGACATGACCGAGCTAA+CGG | + | 37911:38865-38884 | MS.gene66029:CDS | 50.0% | |
| ACTTTCCGTGCAGGATCAAG+TGG | + | 37911:39582-39601 | MS.gene66029:CDS | 50.0% | |
| AGCCCATGGCATCTGTAAGT+AGG | - | 37911:39559-39578 | None:intergenic | 50.0% | |
| AGGATCAAGTGGAAGGACAG+AGG | + | 37911:39593-39612 | MS.gene66029:CDS | 50.0% | |
| ATCTCGGCAGCAATCAGGAA+AGG | - | 37911:38920-38939 | None:intergenic | 50.0% | |
| CGTCCTACTTACAGATGCCA+TGG | + | 37911:39553-39572 | MS.gene66029:CDS | 50.0% | |
| CTCCAAGGGATCCAGTATCT+CGG | - | 37911:38936-38955 | None:intergenic | 50.0% | |
| GACACAAGAGGTTCGAGGTT+TGG | + | 37911:38704-38723 | MS.gene66029:CDS | 50.0% | |
| GCATCATGAACTTCGGGATG+AGG | - | 37911:39115-39134 | None:intergenic | 50.0% | |
| GCATCTGTAAGTAGGACGGT+GGG | - | 37911:39551-39570 | None:intergenic | 50.0% | |
| GCGTCTAAGTTACAACGGAC+AGG | + | 37911:38817-38836 | MS.gene66029:CDS | 50.0% | |
| GCTGTTGCCATGGGTAACAA+TGG | + | 37911:39277-39296 | MS.gene66029:CDS | 50.0% | |
| GTGAAAACATGGGTGCAGGT+GGG | + | 37911:39299-39318 | MS.gene66029:CDS | 50.0% | |
| GTTGTCTTCATCGGATGACG+AGG | - | 37911:38978-38997 | None:intergenic | 50.0% | |
| TCATGAACTTCGGGATGAGG+AGG | - | 37911:39112-39131 | None:intergenic | 50.0% | |
| TCCTTCCACTTGATCCTGCA+CGG | - | 37911:39590-39609 | None:intergenic | 50.0% | |
| TCGGGATGAGGAGGAGAATT+GGG | - | 37911:39103-39122 | None:intergenic | 50.0% | |
| TTCAACTCCTGTGGCAGAAC+CGG | + | 37911:39054-39073 | MS.gene66029:CDS | 50.0% | |
| TTCGGGATGAGGAGGAGAAT+TGG | - | 37911:39104-39123 | None:intergenic | 50.0% | |
| ! | ATTTGTTACGGAGAACCGGC+TGG | + | 37911:39658-39677 | MS.gene66029:CDS | 50.0% |
| ! | CCTGTCTCCCTTTTGGTATC+CGG | + | 37911:38037-38056 | MS.gene66029:intron | 50.0% |
| ! | GGACGCGGACCTTTTTCTAT+AGG | + | 37911:38747-38766 | MS.gene66029:CDS | 50.0% |
| !! | AGGCGGATTTTGGGCATCAT+CGG | - | 37911:39158-39177 | None:intergenic | 50.0% |
| ACATCAGCATCCGCTGGATC+CGG | - | 37911:39076-39095 | None:intergenic | 55.0% | |
| CCAGTATCTCGGCAGCAATC+AGG | - | 37911:38925-38944 | None:intergenic | 55.0% | |
| CCGGATACCAAAAGGGAGAC+AGG | - | 37911:38040-38059 | None:intergenic | 55.0% | |
| CCTCTCACGAACCGTTAGCT+CGG | - | 37911:38879-38898 | None:intergenic | 55.0% | |
| CCTGATTGCTGCCGAGATAC+TGG | + | 37911:38922-38941 | MS.gene66029:CDS | 55.0% | |
| CGAGATACTGGATCCCTTGG+AGG | + | 37911:38934-38953 | MS.gene66029:CDS | 55.0% | |
| CGGCAGCAATCAGGAAAGGA+GGG | - | 37911:38916-38935 | None:intergenic | 55.0% | |
| GAAATGCCGCTGGAGGAAGA+TGG | - | 37911:39619-39638 | None:intergenic | 55.0% | |
| GATGCTGCTGCTGTTGCCAT+GGG | + | 37911:39268-39287 | MS.gene66029:CDS | 55.0% | |
| GCACGGAAAGTCAAAGCCCA+TGG | - | 37911:39573-39592 | None:intergenic | 55.0% | |
| GCAGTATGAGACCTAACGGC+TGG | - | 37911:38799-38818 | None:intergenic | 55.0% | |
| GGACAGGGGCTGTTCTACAT+GGG | + | 37911:38833-38852 | MS.gene66029:CDS | 55.0% | |
| GGCATCTGTAAGTAGGACGG+TGG | - | 37911:39552-39571 | None:intergenic | 55.0% | |
| GGTGAAAACATGGGTGCAGG+TGG | + | 37911:39298-39317 | MS.gene66029:CDS | 55.0% | |
| GGTTCTGCCACAGGAGTTGA+AGG | - | 37911:39055-39074 | None:intergenic | 55.0% | |
| GTATGAGACCTAACGGCTGG+TGG | - | 37911:38796-38815 | None:intergenic | 55.0% | |
| GTATGCTAGGGAAGCTCGGA+TGG | + | 37911:39733-39752 | MS.gene66029:CDS | 55.0% | |
| TCATAGGAAGTGAGGGGACG+CGG | + | 37911:38732-38751 | MS.gene66029:CDS | 55.0% | |
| TCCGTGCAGGATCAAGTGGA+AGG | + | 37911:39586-39605 | MS.gene66029:CDS | 55.0% | |
| TCCTCCAGCGGCATTTCTCA+GGG | + | 37911:39622-39641 | MS.gene66029:CDS | 55.0% | |
| TCGGCAGCAATCAGGAAAGG+AGG | - | 37911:38917-38936 | None:intergenic | 55.0% | |
| TCTGCCACAGGAGTTGAAGG+AGG | - | 37911:39052-39071 | None:intergenic | 55.0% | |
| TCTGTAAGTAGGACGGTGGG+CGG | - | 37911:39548-39567 | None:intergenic | 55.0% | |
| TGATGCTGCTGCTGTTGCCA+TGG | + | 37911:39267-39286 | MS.gene66029:CDS | 55.0% | |
| TGCACCTCCTTCAACTCCTG+TGG | + | 37911:39045-39064 | MS.gene66029:CDS | 55.0% | |
| TGCCGAGATACTGGATCCCT+TGG | + | 37911:38931-38950 | MS.gene66029:CDS | 55.0% | |
| TGGGCATCATCGGCATTGTG+TGG | - | 37911:39148-39167 | None:intergenic | 55.0% | |
| TTCCTCCAGCGGCATTTCTC+AGG | + | 37911:39621-39640 | MS.gene66029:CDS | 55.0% | |
| ! | ACAGGAGTTGAAGGAGGTGC+AGG | - | 37911:39046-39065 | None:intergenic | 55.0% |
| ! | CACTTTTCCCAACTGCCAGC+CGG | - | 37911:39676-39695 | None:intergenic | 55.0% |
| ! | TGGGCTTTGACTTTCCGTGC+AGG | + | 37911:39573-39592 | MS.gene66029:CDS | 55.0% |
| CAGAGGCCATCTTCCTCCAG+CGG | + | 37911:39610-39629 | MS.gene66029:CDS | 60.0% | |
| CATGGGTGCAGGTGGGAATG+TGG | + | 37911:39306-39325 | MS.gene66029:CDS | 60.0% | |
| CCGAGCTAACGGTTCGTGAG+AGG | + | 37911:38876-38895 | MS.gene66029:CDS | 60.0% | |
| CCTCCAGCGGCATTTCTCAG+GGG | + | 37911:39623-39642 | MS.gene66029:CDS | 60.0% | |
| CGGACAGGGGCTGTTCTACA+TGG | + | 37911:38832-38851 | MS.gene66029:CDS | 60.0% | |
| GAGCACTTCCACCAGCCGTT+AGG | + | 37911:38785-38804 | MS.gene66029:CDS | 60.0% | |
| GGTGCAACATCAGCATCCGC+TGG | - | 37911:39082-39101 | None:intergenic | 60.0% | |
| TGTGGCAGAACCGGATCCAG+CGG | + | 37911:39063-39082 | MS.gene66029:CDS | 60.0% | |
| ! | GGAGTTGAAGGAGGTGCAGG+AGG | - | 37911:39043-39062 | None:intergenic | 60.0% |
| ACGGAGAACCGGCTGGCAGT+TGG | + | 37911:39665-39684 | MS.gene66029:CDS | 65.0% | |
| CAGCGGCATTTCTCAGGGGC+GGG | + | 37911:39627-39646 | MS.gene66029:CDS | 65.0% | |
| CCAGCGGCATTTCTCAGGGG+CGG | + | 37911:39626-39645 | MS.gene66029:CDS | 65.0% | |
| CCCCTGAGAAATGCCGCTGG+AGG | - | 37911:39626-39645 | None:intergenic | 65.0% | |
| CGGAGAACCGGCTGGCAGTT+GGG | + | 37911:39666-39685 | MS.gene66029:CDS | 65.0% | |
| GCTGGATCCGGTTCTGCCAC+AGG | - | 37911:39064-39083 | None:intergenic | 65.0% | |
| CCGCCCCTGAGAAATGCCGC+TGG | - | 37911:39629-39648 | None:intergenic | 70.0% | |
| CGGCATTTCTCAGGGGCGGG+TGG | + | 37911:39630-39649 | MS.gene66029:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37911 | gene | 37895 | 39763 | 37895 | ID=MS.gene66029 |
| 37911 | mRNA | 37895 | 39763 | 37895 | ID=MS.gene66029.t1;Parent=MS.gene66029 |
| 37911 | exon | 37895 | 37945 | 37895 | ID=MS.gene66029.t1.exon1;Parent=MS.gene66029.t1 |
| 37911 | CDS | 37895 | 37945 | 37895 | ID=cds.MS.gene66029.t1;Parent=MS.gene66029.t1 |
| 37911 | exon | 38632 | 39396 | 38632 | ID=MS.gene66029.t1.exon2;Parent=MS.gene66029.t1 |
| 37911 | CDS | 38632 | 39396 | 38632 | ID=cds.MS.gene66029.t1;Parent=MS.gene66029.t1 |
| 37911 | exon | 39500 | 39763 | 39500 | ID=MS.gene66029.t1.exon3;Parent=MS.gene66029.t1 |
| 37911 | CDS | 39500 | 39763 | 39500 | ID=cds.MS.gene66029.t1;Parent=MS.gene66029.t1 |
| Gene Sequence |
| Protein sequence |
