AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66114

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66114.t1 MTR_7g057230 83.164 493 47 1 1 457 165 657 0.0 823
MS.gene66114.t1 MTR_6g047750 54.233 378 153 6 91 456 292 661 3.47e-132 396
MS.gene66114.t1 MTR_4g074310 63.043 230 51 3 96 325 1 196 2.03e-89 271
MS.gene66114.t1 MTR_3g089055 38.009 442 250 10 27 456 135 564 2.06e-81 262
MS.gene66114.t1 MTR_3g089055 38.009 442 250 10 27 456 140 569 3.37e-81 262
MS.gene66114.t1 MTR_4g074320 65.957 188 28 1 75 226 1 188 1.77e-77 239
MS.gene66114.t1 MTR_1g029420 37.674 430 250 8 38 456 169 591 2.85e-77 252
MS.gene66114.t1 MTR_2g082090 34.031 382 238 5 84 456 202 578 2.10e-63 215
MS.gene66114.t1 MTR_4g133660 33.766 385 238 7 83 456 164 542 2.24e-62 211
MS.gene66114.t1 MTR_3g056110 33.626 342 213 6 122 456 207 541 3.29e-57 197
MS.gene66114.t1 MTR_5g097480 31.616 427 268 9 48 456 123 543 7.99e-57 197
MS.gene66114.t1 MTR_5g094450 32.808 381 232 10 92 456 159 531 5.62e-55 191
MS.gene66114.t1 MTR_2g026250 30.513 390 250 8 80 456 214 595 2.65e-52 186
MS.gene66114.t1 MTR_8g442410 30.088 339 212 8 134 456 195 524 9.90e-35 136
MS.gene66114.t1 MTR_3g065980 31.392 309 183 7 167 456 241 539 9.18e-34 133
MS.gene66114.t1 MTR_7g027190 31.438 299 180 8 165 456 392 672 1.15e-32 131
MS.gene66114.t1 MTR_4g102790 33.585 265 159 9 202 456 404 661 3.69e-29 121
MS.gene66114.t1 MTR_4g104020 28.618 304 205 6 157 456 224 519 6.41e-29 119
MS.gene66114.t1 MTR_4g064160 26.741 359 244 7 110 456 332 683 8.52e-29 120
MS.gene66114.t1 MTR_4g064200 28.425 292 191 7 175 456 366 649 2.33e-27 115
MS.gene66114.t1 MTR_2g097350 27.526 287 197 5 175 456 359 639 7.90e-26 110
MS.gene66114.t1 MTR_4g077760 36.515 241 134 9 175 409 276 503 5.70e-25 108
MS.gene66114.t1 MTR_2g097310 26.389 288 201 5 175 457 357 638 8.93e-25 107
MS.gene66114.t1 MTR_4g097080 27.431 288 189 6 179 456 225 502 1.18e-24 106
MS.gene66114.t1 MTR_2g097410 26.531 294 194 7 175 456 457 740 1.36e-24 107
MS.gene66114.t1 MTR_4g064150 23.418 316 228 6 150 457 424 733 2.38e-24 106
MS.gene66114.t1 MTR_7g109580 27.799 259 179 3 202 456 299 553 7.39e-24 104
MS.gene66114.t1 MTR_2g097380 27.113 284 187 7 175 456 295 560 1.19e-23 104
MS.gene66114.t1 MTR_7g062120 24.407 295 198 6 175 456 457 739 2.01e-23 103
MS.gene66114.t1 MTR_4g064180 23.962 313 224 6 150 454 317 623 2.33e-23 103
MS.gene66114.t1 MTR_2g097390 25.436 287 203 5 175 456 360 640 2.96e-23 103
MS.gene66114.t1 MTR_2g097463 26.812 276 182 5 194 456 386 654 8.87e-23 102
MS.gene66114.t1 MTR_4g064120 23.003 313 227 6 150 454 317 623 2.95e-22 100
MS.gene66114.t1 MTR_2g097473 26.619 278 181 6 195 456 383 653 5.05e-22 99.8
MS.gene66114.t1 MTR_3g022830 26.608 342 190 9 168 456 102 435 8.74e-22 97.8
MS.gene66114.t1 MTR_2g034260 30.996 271 170 8 196 456 320 583 1.91e-21 97.4
MS.gene66114.t1 MTR_7g074650 27.742 310 203 9 151 456 499 791 2.30e-21 97.8
MS.gene66114.t1 MTR_2g034250 28.966 290 192 6 176 456 300 584 2.66e-21 97.1
MS.gene66114.t1 MTR_2g097467 25.436 287 192 6 184 456 375 653 4.08e-21 96.7
MS.gene66114.t1 MTR_2g034280 27.336 289 199 5 175 456 290 574 7.83e-21 95.9
MS.gene66114.t1 MTR_5g015490 26.552 290 195 8 179 456 206 489 1.84e-18 88.2
MS.gene66114.t1 MTR_4g076020 27.682 289 170 12 175 456 172 428 2.38e-18 87.4
MS.gene66114.t1 MTR_1g069725 26.667 300 198 9 163 456 179 462 1.66e-17 85.1
MS.gene66114.t1 MTR_4g076140 34.667 150 93 3 311 456 320 468 4.87e-17 83.6
MS.gene66114.t1 MTR_5g009080 36.184 152 88 4 311 456 329 477 9.36e-17 82.8
MS.gene66114.t1 MTR_1g096030 24.810 395 247 12 95 456 81 458 2.78e-16 81.3
MS.gene66114.t1 MTR_5g015950 25.000 320 196 7 175 456 132 445 2.91e-16 81.3
MS.gene66114.t1 MTR_3g053270 25.000 320 196 7 175 456 132 445 2.91e-16 81.3
MS.gene66114.t1 MTR_2g089100 21.975 405 258 13 95 456 53 442 5.49e-15 77.4
MS.gene66114.t1 MTR_4g026485 24.800 375 248 12 95 456 270 623 6.89e-14 74.3
MS.gene66114.t1 MTR_1g086970 24.265 272 197 4 189 456 211 477 3.72e-13 71.6
MS.gene66114.t1 MTR_4g095500 23.265 245 158 3 242 456 223 467 2.27e-11 66.2
MS.gene66114.t1 MTR_2g099110 28.910 211 134 5 179 385 166 364 3.24e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66114.t1 AT5G52510 46.083 434 175 10 72 456 216 639 1.47e-116 355
MS.gene66114.t1 AT1G21450 35.154 421 251 7 47 456 183 592 2.11e-70 234
MS.gene66114.t1 AT5G48150 36.127 346 198 8 123 456 155 489 2.43e-60 205
MS.gene66114.t1 AT5G48150 36.127 346 198 8 123 456 155 489 2.43e-60 205
MS.gene66114.t1 AT5G48150 36.127 346 198 8 123 456 155 489 2.43e-60 205
MS.gene66114.t1 AT5G48150 36.127 346 198 8 123 456 155 489 2.43e-60 205
MS.gene66114.t1 AT2G04890 32.620 374 231 8 92 456 51 412 1.02e-57 196
MS.gene66114.t1 AT4G17230 31.604 424 266 9 48 456 110 524 5.62e-55 191
MS.gene66114.t1 AT4G17230 31.604 424 266 9 48 456 110 524 5.62e-55 191
MS.gene66114.t1 AT1G50600 33.913 345 206 7 123 456 192 525 5.76e-55 191
MS.gene66114.t1 AT1G50600 33.913 345 206 7 123 456 263 596 2.43e-54 191
MS.gene66114.t1 AT1G50600 33.913 345 206 7 123 456 267 600 2.46e-54 191
MS.gene66114.t1 AT3G03450 33.564 289 177 6 174 456 265 544 1.87e-36 141
MS.gene66114.t1 AT1G66350 31.788 302 185 9 161 456 219 505 8.23e-36 139
MS.gene66114.t1 AT3G50650 32.550 298 175 6 175 456 254 541 1.48e-32 130
MS.gene66114.t1 AT5G17490 31.864 295 186 7 167 456 231 515 1.51e-32 130
MS.gene66114.t1 AT2G01570 32.646 291 176 8 174 456 302 580 2.03e-30 124
MS.gene66114.t1 AT1G14920 32.302 291 177 9 174 456 250 528 2.18e-30 124
MS.gene66114.t1 AT5G66770 35.270 241 142 5 175 409 300 532 5.97e-30 123
MS.gene66114.t1 AT2G29065 25.132 378 266 8 95 457 253 628 4.86e-29 120
MS.gene66114.t1 AT2G29060 26.207 290 202 5 175 456 406 691 1.56e-27 116
MS.gene66114.t1 AT2G29060 26.207 290 202 5 175 456 369 654 1.58e-27 116
MS.gene66114.t1 AT1G07530 25.510 294 209 4 171 457 475 765 1.94e-27 116
MS.gene66114.t1 AT2G37650 24.274 379 256 8 95 456 336 700 3.36e-25 109
MS.gene66114.t1 AT2G37650 24.274 379 256 8 95 456 348 712 3.51e-25 109
MS.gene66114.t1 AT5G59450 26.471 340 222 11 135 456 268 597 7.02e-24 105
MS.gene66114.t1 AT3G46600 26.552 290 196 6 176 456 89 370 2.67e-23 101
MS.gene66114.t1 AT4G37650 24.878 410 262 12 76 456 136 528 4.06e-23 102
MS.gene66114.t1 AT1G50420 25.170 441 231 14 95 456 58 478 4.59e-23 102
MS.gene66114.t1 AT1G07520 25.170 294 200 7 176 457 409 694 5.77e-23 102
MS.gene66114.t1 AT1G07520 25.170 294 200 7 176 457 408 693 5.81e-23 102
MS.gene66114.t1 AT1G07520 25.170 294 200 7 176 457 244 529 8.79e-23 101
MS.gene66114.t1 AT1G07520 25.170 294 200 7 176 457 244 529 8.79e-23 101
MS.gene66114.t1 AT3G46600 26.552 290 196 6 176 456 297 578 1.09e-22 101
MS.gene66114.t1 AT3G46600 26.552 290 196 6 176 456 265 546 1.65e-22 100
MS.gene66114.t1 AT3G54220 27.931 290 188 9 171 456 377 649 1.57e-20 95.1
MS.gene66114.t1 AT1G63100 31.905 210 137 5 202 410 388 592 4.71e-20 93.6
MS.gene66114.t1 AT1G63100 31.905 210 137 5 202 410 363 567 5.58e-20 93.2
MS.gene66114.t1 AT5G41920 27.551 294 183 10 171 456 128 399 5.65e-18 86.3
MS.gene66114.t1 AT3G49950 24.372 398 260 9 88 457 24 408 2.53e-17 84.3
MS.gene66114.t1 AT1G55580 26.014 296 197 7 175 456 157 444 1.24e-14 76.3
MS.gene66114.t1 AT5G67411 28.729 181 115 5 228 395 19 198 2.51e-11 63.5
MS.gene66114.t1 AT3G13840 24.497 298 202 12 167 456 227 509 9.35e-11 64.3

Find 77 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTCGATAGTTGGTTAT+TGG 0.173769 7.4:+45591021 MS.gene66114:CDS
CTCTCAATGTTGCATCTTCT+TGG 0.201520 7.4:+45591992 MS.gene66114:CDS
TTGCTTCAAGCTCTCTTTCA+TGG 0.207322 7.4:+45591162 MS.gene66114:CDS
GAATGGTCACTAGTTGTTTC+TGG 0.207414 7.4:+45590760 MS.gene66114:CDS
GAAATCCATCGGAGAACTTT+TGG 0.212473 7.4:+45591381 MS.gene66114:CDS
TCCAAGCATTGTCACAGTTT+TGG 0.258937 7.4:+45591609 MS.gene66114:CDS
GAGAGATTAAAGCTTGAAAA+AGG 0.266629 7.4:+45591745 MS.gene66114:CDS
TTCAATTGCTGAAGGCAAAA+TGG 0.270126 7.4:+45590946 MS.gene66114:CDS
GGCTTCAAGGATTGCGATAT+TGG 0.275042 7.4:-45591188 None:intergenic
GAGTTAGTTCGGTGAGATTT+AGG 0.277013 7.4:-45591453 None:intergenic
TGAGACTCGTATGAAGTTCT+TGG 0.280652 7.4:-45591297 None:intergenic
TCACACTCTTTGTTGTATCT+TGG 0.314011 7.4:-45591216 None:intergenic
CGTGCACGCATGAGCATGGC+TGG 0.338695 7.4:+45591847 MS.gene66114:CDS
TCATACATCATCTTCTTGTC+TGG 0.342721 7.4:-45590697 None:intergenic
GCATGAGCATGGCTGGGTTT+AGG 0.345416 7.4:+45591854 MS.gene66114:CDS
TTGCTGAACAATTCCGCAAC+TGG 0.346117 7.4:-45591097 None:intergenic
GGTCGAGAAGCTGAGAGTTT+AGG 0.349907 7.4:+45591403 MS.gene66114:CDS
TGAAGGATTGAATTGTTCAT+AGG 0.353656 7.4:-45590601 None:intergenic
ATTGAATTGTTCATAGGTAA+TGG 0.359237 7.4:-45590595 None:intergenic
TTGAATTGTTCATAGGTAAT+GGG 0.362850 7.4:-45590594 None:intergenic
TGGAGTTTGCTTTGGTTGGA+TGG 0.363970 7.4:+45591963 MS.gene66114:CDS
AGCTTCCATTAACGATTGTT+TGG 0.384672 7.4:-45590918 None:intergenic
AATGGTCACTAGTTGTTTCT+GGG 0.387462 7.4:+45590761 MS.gene66114:CDS
CAATGACTCGCGAGTTAGTT+CGG 0.393659 7.4:-45591464 None:intergenic
ATCGTGATCTTCAACATTGC+TGG 0.402461 7.4:-45591328 None:intergenic
TTGTCTGGGTCATAACTTGT+TGG 0.402653 7.4:-45590682 None:intergenic
ATGGTGGAGTTTGCTTTGGT+TGG 0.406060 7.4:+45591959 MS.gene66114:CDS
GGAGTTTGCTTTGGTTGGAT+GGG 0.411286 7.4:+45591964 MS.gene66114:CDS
GTTACTGACTCAGTAACTTT+CGG 0.416851 7.4:-45591892 None:intergenic
TATCTTGGAATGCGGCTTCA+AGG 0.418126 7.4:-45591201 None:intergenic
CAAAACTAAAATTCGATAGT+TGG 0.427464 7.4:+45591014 MS.gene66114:CDS
AACAATTCCGCAACTGGAGG+TGG 0.434762 7.4:-45591091 None:intergenic
GCACAGTAATTCTGCTACCT+TGG 0.435103 7.4:-45591927 None:intergenic
TGTTTCTGACCATTCGTTGT+TGG 0.451392 7.4:-45590794 None:intergenic
AAGCTGAGAGTTTAGGAATC+GGG 0.451634 7.4:+45591410 MS.gene66114:CDS
CAGTAACTTTCGGACTCATC+GGG 0.453689 7.4:-45591882 None:intergenic
GAGAATGGTGGAGTTTGCTT+TGG 0.469489 7.4:+45591955 MS.gene66114:CDS
TCCAAAACTGTGACAATGCT+TGG 0.490294 7.4:-45591610 None:intergenic
GTGTATGTGCGAAACTAAAG+AGG 0.494918 7.4:-45590985 None:intergenic
TCAGTAACTTTCGGACTCAT+CGG 0.497001 7.4:-45591883 None:intergenic
TCAGCCACACGTGAAACTAA+TGG 0.502475 7.4:-45591658 None:intergenic
GAAGCTGAGAGTTTAGGAAT+CGG 0.518502 7.4:+45591409 MS.gene66114:CDS
GAACTTCATACGAGTCTCAA+TGG 0.519117 7.4:+45591301 MS.gene66114:CDS
GAGAGAGTGATTTCACTTGA+CGG 0.522514 7.4:-45591588 None:intergenic
TGCAGTTGCTTGTGAAGGGA+GGG 0.531185 7.4:+45591789 MS.gene66114:CDS
TCGACCCAAAAGTTCTCCGA+TGG 0.533012 7.4:-45591386 None:intergenic
CTTTGTTGTATCTTGGAATG+CGG 0.536014 7.4:-45591209 None:intergenic
AATCGGGTTTGAATTCAAAC+CGG 0.540939 7.4:+45591426 MS.gene66114:CDS
CATACATCATCTTCTTGTCT+GGG 0.542385 7.4:-45590696 None:intergenic
TCGGTGAGATTTAGGTTCAC+CGG 0.544255 7.4:-45591445 None:intergenic
AGAATCTATTGAATTTGCGA+TGG 0.545507 7.4:+45591708 MS.gene66114:CDS
ACATTGATGATGAAGAAGAA+TGG 0.574603 7.4:+45590743 MS.gene66114:CDS
ATGGCGTGCACGCATGAGCA+TGG 0.579124 7.4:+45591843 MS.gene66114:CDS
AAGGGAGGGATCGTGTGGAA+AGG 0.587033 7.4:+45591803 MS.gene66114:CDS
GTGCACGCATGAGCATGGCT+GGG 0.597641 7.4:+45591848 MS.gene66114:CDS
GCTGCCACTTCAATTGCTGA+AGG 0.603088 7.4:+45590938 MS.gene66114:CDS
ATGCAGTTGCTTGTGAAGGG+AGG 0.607004 7.4:+45591788 MS.gene66114:CDS
CTGAACAATTCCGCAACTGG+AGG 0.608240 7.4:-45591094 None:intergenic
ATTACTGTGCATGAAGAGAA+TGG 0.610201 7.4:+45591940 MS.gene66114:CDS
TGAAAATCCACCTCCAGTTG+CGG 0.610781 7.4:+45591084 MS.gene66114:CDS
TTTGCCTTCAGCAATTGAAG+TGG 0.611526 7.4:-45590942 None:intergenic
TGTGAAGAATCTCTGTGTTG+TGG 0.612679 7.4:+45591234 MS.gene66114:CDS
ATAAATGCAGTTGCTTGTGA+AGG 0.616404 7.4:+45591784 MS.gene66114:CDS
AAAGAGAAGTTGAGTTGACT+CGG 0.617772 7.4:-45591128 None:intergenic
TTATGAACACCAACAACGAA+TGG 0.618414 7.4:+45590785 MS.gene66114:CDS
ACTAGATGAGAGAGTGTCAA+CGG 0.620039 7.4:+45591543 MS.gene66114:CDS
GATGACAATCTGAAATCCAT+CGG 0.638005 7.4:+45591370 MS.gene66114:CDS
GTCATTGAATTGTGACTCAG+AGG 0.645367 7.4:+45591480 MS.gene66114:CDS
AGGCAAAATGGATTATGCGA+AGG 0.646229 7.4:+45590958 MS.gene66114:CDS
AACACCATTAGTTTCACGTG+TGG 0.647563 7.4:+45591654 MS.gene66114:CDS
GTAACTTCAAGATTCATCCA+AGG 0.655646 7.4:+45591910 MS.gene66114:CDS
TGGATTTCAGATTGTCATCG+TGG 0.656125 7.4:-45591366 None:intergenic
TAAATGCAGTTGCTTGTGAA+GGG 0.661760 7.4:+45591785 MS.gene66114:CDS
TGACGGAGAAGTGTGTCACG+TGG 0.698381 7.4:-45591571 None:intergenic
TTGTGAAGGGAGGGATCGTG+TGG 0.715844 7.4:+45591798 MS.gene66114:CDS
GTTGAAGATCACGATTGTGA+CGG 0.729260 7.4:+45591336 MS.gene66114:CDS
ACTGTGCATGAAGAGAATGG+TGG 0.760237 7.4:+45591943 MS.gene66114:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATCGAATTTTAGTTTT+GGG - chr7.4:45591015-45591034 None:intergenic 20.0%
! AAAATTCGATAGTTGGTTAT+TGG + chr7.4:45591021-45591040 MS.gene66114:CDS 25.0%
! ATTGAATTGTTCATAGGTAA+TGG - chr7.4:45590598-45590617 None:intergenic 25.0%
! CAAAACTAAAATTCGATAGT+TGG + chr7.4:45591014-45591033 MS.gene66114:CDS 25.0%
! TTGAATTGTTCATAGGTAAT+GGG - chr7.4:45590597-45590616 None:intergenic 25.0%
!! AGCAAAATCAGAATTTTCAT+AGG - chr7.4:45590641-45590660 None:intergenic 25.0%
!!! AATGAAATAGGTTTTTGGAT+GGG - chr7.4:45590850-45590869 None:intergenic 25.0%
!!! CAACTATCGAATTTTAGTTT+TGG - chr7.4:45591016-45591035 None:intergenic 25.0%
!!! GTAATGGGTTTTGAATATTA+GGG - chr7.4:45590582-45590601 None:intergenic 25.0%
AAAGAAGGTGAGAATGAAAT+AGG - chr7.4:45590862-45590881 None:intergenic 30.0%
AGAATCTATTGAATTTGCGA+TGG + chr7.4:45591708-45591727 MS.gene66114:CDS 30.0%
GAGAGATTAAAGCTTGAAAA+AGG + chr7.4:45591745-45591764 MS.gene66114:CDS 30.0%
TGAAGGATTGAATTGTTCAT+AGG - chr7.4:45590604-45590623 None:intergenic 30.0%
! AGGAAAAATTCGCATTTTGA+AGG - chr7.4:45590621-45590640 None:intergenic 30.0%
! GTGAGAATGAAATAGGTTTT+TGG - chr7.4:45590855-45590874 None:intergenic 30.0%
! GTTTTCCAAACAATCGTTAA+TGG + chr7.4:45590913-45590932 MS.gene66114:CDS 30.0%
!! ACATTGATGATGAAGAAGAA+TGG + chr7.4:45590743-45590762 MS.gene66114:CDS 30.0%
!! ATGAAATAGGTTTTTGGATG+GGG - chr7.4:45590849-45590868 None:intergenic 30.0%
!! GAATGAAATAGGTTTTTGGA+TGG - chr7.4:45590851-45590870 None:intergenic 30.0%
!! GGTGGATTTTCAATGTTATT+CGG - chr7.4:45591076-45591095 None:intergenic 30.0%
!! GTGGATTTTGATATTGGTAA+TGG + chr7.4:45591253-45591272 MS.gene66114:CDS 30.0%
!! TGTGTTGTGGATTTTGATAT+TGG + chr7.4:45591247-45591266 MS.gene66114:CDS 30.0%
!!! GGTAATGGGTTTTGAATATT+AGG - chr7.4:45590583-45590602 None:intergenic 30.0%
AATCGGGTTTGAATTCAAAC+CGG + chr7.4:45591426-45591445 MS.gene66114:CDS 35.0%
AGCTTCCATTAACGATTGTT+TGG - chr7.4:45590921-45590940 None:intergenic 35.0%
ATAAATGCAGTTGCTTGTGA+AGG + chr7.4:45591784-45591803 MS.gene66114:CDS 35.0%
ATTACTGTGCATGAAGAGAA+TGG + chr7.4:45591940-45591959 MS.gene66114:CDS 35.0%
CATACATCATCTTCTTGTCT+GGG - chr7.4:45590699-45590718 None:intergenic 35.0%
GTAACTTCAAGATTCATCCA+AGG + chr7.4:45591910-45591929 MS.gene66114:CDS 35.0%
TAAATGCAGTTGCTTGTGAA+GGG + chr7.4:45591785-45591804 MS.gene66114:CDS 35.0%
TCATACATCATCTTCTTGTC+TGG - chr7.4:45590700-45590719 None:intergenic 35.0%
TTATGAACACCAACAACGAA+TGG + chr7.4:45590785-45590804 MS.gene66114:CDS 35.0%
TTCAATTGCTGAAGGCAAAA+TGG + chr7.4:45590946-45590965 MS.gene66114:CDS 35.0%
! AAATCCATCGGAGAACTTTT+GGG + chr7.4:45591382-45591401 MS.gene66114:CDS 35.0%
! CTTTGTTGTATCTTGGAATG+CGG - chr7.4:45591212-45591231 None:intergenic 35.0%
! GATGACAATCTGAAATCCAT+CGG + chr7.4:45591370-45591389 MS.gene66114:CDS 35.0%
! TCACACTCTTTGTTGTATCT+TGG - chr7.4:45591219-45591238 None:intergenic 35.0%
! TTTTGCTGAGATTCAAAGCA+CGG + chr7.4:45590654-45590673 MS.gene66114:CDS 35.0%
!! AAAGAGAAGTTGAGTTGACT+CGG - chr7.4:45591131-45591150 None:intergenic 35.0%
!! AATGGTCACTAGTTGTTTCT+GGG + chr7.4:45590761-45590780 MS.gene66114:CDS 35.0%
!! CAATAGATGGTGGAAAAAGA+AGG - chr7.4:45590877-45590896 None:intergenic 35.0%
!! GTTACTGACTCAGTAACTTT+CGG - chr7.4:45591895-45591914 None:intergenic 35.0%
!! TGGAAAGGTGTGAAGTTTTT+GGG + chr7.4:45591818-45591837 MS.gene66114:CDS 35.0%
AAGCTGAGAGTTTAGGAATC+GGG + chr7.4:45591410-45591429 MS.gene66114:CDS 40.0%
ATCGTGATCTTCAACATTGC+TGG - chr7.4:45591331-45591350 None:intergenic 40.0%
CTCTCAATGTTGCATCTTCT+TGG + chr7.4:45591992-45592011 MS.gene66114:CDS 40.0%
GAACTTCATACGAGTCTCAA+TGG + chr7.4:45591301-45591320 MS.gene66114:CDS 40.0%
GAAGCTGAGAGTTTAGGAAT+CGG + chr7.4:45591409-45591428 MS.gene66114:CDS 40.0%
GAAGCTGGTGAAACAATAGA+TGG - chr7.4:45590890-45590909 None:intergenic 40.0%
GAGAGAGTGATTTCACTTGA+CGG - chr7.4:45591591-45591610 None:intergenic 40.0%
GTCATTGAATTGTGACTCAG+AGG + chr7.4:45591480-45591499 MS.gene66114:CDS 40.0%
GTGTATGTGCGAAACTAAAG+AGG - chr7.4:45590988-45591007 None:intergenic 40.0%
GTTGAAGATCACGATTGTGA+CGG + chr7.4:45591336-45591355 MS.gene66114:CDS 40.0%
TCAGTAACTTTCGGACTCAT+CGG - chr7.4:45591886-45591905 None:intergenic 40.0%
TCCAAAACTGTGACAATGCT+TGG - chr7.4:45591613-45591632 None:intergenic 40.0%
TGAGACTCGTATGAAGTTCT+TGG - chr7.4:45591300-45591319 None:intergenic 40.0%
TGGATTTCAGATTGTCATCG+TGG - chr7.4:45591369-45591388 None:intergenic 40.0%
TGTGAAGAATCTCTGTGTTG+TGG + chr7.4:45591234-45591253 MS.gene66114:CDS 40.0%
TGTTTGGAAAACTCAGAAGC+TGG - chr7.4:45590905-45590924 None:intergenic 40.0%
TTGCTTCAAGCTCTCTTTCA+TGG + chr7.4:45591162-45591181 MS.gene66114:CDS 40.0%
TTTGCCTTCAGCAATTGAAG+TGG - chr7.4:45590945-45590964 None:intergenic 40.0%
! AACACCATTAGTTTCACGTG+TGG + chr7.4:45591654-45591673 MS.gene66114:CDS 40.0%
! GAAATCCATCGGAGAACTTT+TGG + chr7.4:45591381-45591400 MS.gene66114:CDS 40.0%
! GAATGGTCACTAGTTGTTTC+TGG + chr7.4:45590760-45590779 MS.gene66114:CDS 40.0%
! GAGTTAGTTCGGTGAGATTT+AGG - chr7.4:45591456-45591475 None:intergenic 40.0%
! TGTTTCTGACCATTCGTTGT+TGG - chr7.4:45590797-45590816 None:intergenic 40.0%
! TTGTCTGGGTCATAACTTGT+TGG - chr7.4:45590685-45590704 None:intergenic 40.0%
!! ACTAGATGAGAGAGTGTCAA+CGG + chr7.4:45591543-45591562 MS.gene66114:CDS 40.0%
!! AGGCAAAATGGATTATGCGA+AGG + chr7.4:45590958-45590977 MS.gene66114:CDS 40.0%
!! GGTGTGAAGTTTTTGGGAAA+TGG + chr7.4:45591824-45591843 MS.gene66114:CDS 40.0%
!! GTGGAAAGGTGTGAAGTTTT+TGG + chr7.4:45591817-45591836 MS.gene66114:CDS 40.0%
!! TCCAAGCATTGTCACAGTTT+TGG + chr7.4:45591609-45591628 MS.gene66114:CDS 40.0%
ACTGTGCATGAAGAGAATGG+TGG + chr7.4:45591943-45591962 MS.gene66114:CDS 45.0%
CAATGACTCGCGAGTTAGTT+CGG - chr7.4:45591467-45591486 None:intergenic 45.0%
CAGTAACTTTCGGACTCATC+GGG - chr7.4:45591885-45591904 None:intergenic 45.0%
GCACAGTAATTCTGCTACCT+TGG - chr7.4:45591930-45591949 None:intergenic 45.0%
GCTGGTGAAACAATAGATGG+TGG - chr7.4:45590887-45590906 None:intergenic 45.0%
GGCTTCAAGGATTGCGATAT+TGG - chr7.4:45591191-45591210 None:intergenic 45.0%
TATCTTGGAATGCGGCTTCA+AGG - chr7.4:45591204-45591223 None:intergenic 45.0%
TCAGCCACACGTGAAACTAA+TGG - chr7.4:45591661-45591680 None:intergenic 45.0%
TGAAAATCCACCTCCAGTTG+CGG + chr7.4:45591084-45591103 MS.gene66114:CDS 45.0%
TTGCTGAACAATTCCGCAAC+TGG - chr7.4:45591100-45591119 None:intergenic 45.0%
! ATGGTGGAGTTTGCTTTGGT+TGG + chr7.4:45591959-45591978 MS.gene66114:CDS 45.0%
! GAGAATGGTGGAGTTTGCTT+TGG + chr7.4:45591955-45591974 MS.gene66114:CDS 45.0%
! GGAGTTTGCTTTGGTTGGAT+GGG + chr7.4:45591964-45591983 MS.gene66114:CDS 45.0%
! TCGGTGAGATTTAGGTTCAC+CGG - chr7.4:45591448-45591467 None:intergenic 45.0%
! TGGAGTTTGCTTTGGTTGGA+TGG + chr7.4:45591963-45591982 MS.gene66114:CDS 45.0%
AACAATTCCGCAACTGGAGG+TGG - chr7.4:45591094-45591113 None:intergenic 50.0%
ATGCAGTTGCTTGTGAAGGG+AGG + chr7.4:45591788-45591807 MS.gene66114:CDS 50.0%
CTGAACAATTCCGCAACTGG+AGG - chr7.4:45591097-45591116 None:intergenic 50.0%
GGTCGAGAAGCTGAGAGTTT+AGG + chr7.4:45591403-45591422 MS.gene66114:CDS 50.0%
TCGACCCAAAAGTTCTCCGA+TGG - chr7.4:45591389-45591408 None:intergenic 50.0%
TGCAGTTGCTTGTGAAGGGA+GGG + chr7.4:45591789-45591808 MS.gene66114:CDS 50.0%
! GCTGCCACTTCAATTGCTGA+AGG + chr7.4:45590938-45590957 MS.gene66114:CDS 50.0%
AAGGGAGGGATCGTGTGGAA+AGG + chr7.4:45591803-45591822 MS.gene66114:CDS 55.0%
GCATGAGCATGGCTGGGTTT+AGG + chr7.4:45591854-45591873 MS.gene66114:CDS 55.0%
TTGTGAAGGGAGGGATCGTG+TGG + chr7.4:45591798-45591817 MS.gene66114:CDS 55.0%
! TGACGGAGAAGTGTGTCACG+TGG - chr7.4:45591574-45591593 None:intergenic 55.0%
ATGGCGTGCACGCATGAGCA+TGG + chr7.4:45591843-45591862 MS.gene66114:CDS 60.0%
GTGCACGCATGAGCATGGCT+GGG + chr7.4:45591848-45591867 MS.gene66114:CDS 60.0%
CGTGCACGCATGAGCATGGC+TGG + chr7.4:45591847-45591866 MS.gene66114:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 45590562 45592020 45590562 ID=MS.gene66114
chr7.4 mRNA 45590562 45592020 45590562 ID=MS.gene66114.t1;Parent=MS.gene66114
chr7.4 exon 45590562 45590820 45590562 ID=MS.gene66114.t1.exon1;Parent=MS.gene66114.t1
chr7.4 CDS 45590562 45590820 45590562 ID=cds.MS.gene66114.t1;Parent=MS.gene66114.t1
chr7.4 exon 45590906 45592020 45590906 ID=MS.gene66114.t1.exon2;Parent=MS.gene66114.t1
chr7.4 CDS 45590906 45592020 45590906 ID=cds.MS.gene66114.t1;Parent=MS.gene66114.t1
Gene Sequence

>MS.gene66114

ATGTCTCTTCGCTCAAACCCTAATATTCAAAACCCATTACCTATGAACAATTCAATCCTTCAAAATGCGAATTTTTCCTATGAAAATTCTGATTTTGCTGAGATTCAAAGCACGGTAGTTCCAACAAGTTATGACCCAGACAAGAAGATGATGTATGAGTTAGAGAAAAAGCTTCATGAAGACATTGATGATGAAGAAGAATGGTCACTAGTTGTTTCTGGGATTATGAACACCAACAACGAATGGTCAGAAACAATTCGTAATTTGAATAGTTTCGAGCAAAACCCCATCCAAAAACCTATTTCATTCTCACCTTCTTTTTCCACCATCTATTGTTTCACCAGCTTCTGAGTTTTCCAAACAATCGTTAATGGAAGCTGCCACTTCAATTGCTGAAGGCAAAATGGATTATGCGAAGGAGATCCTCTTTAGTTTCGCACATACACAAAACCCAAAACTAAAATTCGATAGTTGGTTATTGGATTGCATTGCTTCAGCGCTGAAATTGCGTCCGAATAACATTGAAAATCCACCTCCAGTTGCGGAATTGTTCAGCAAAGAACATGCCGAGTCAACTCAACTTCTCTTTGATCACTCTCTTTGCTTCAAGCTCTCTTTCATGGCTGCCAATATCGCAATCCTTGAAGCCGCATTCCAAGATACAACAAAGAGTGTGAAGAATCTCTGTGTTGTGGATTTTGATATTGGTAATGGAAAACAATACATCAATCTGCTCCAAGAACTTCATACGAGTCTCAATGGTTCACCAGCAATGTTGAAGATCACGATTGTGACGGTGAACATCCACGATGACAATCTGAAATCCATCGGAGAACTTTTGGGTCGAGAAGCTGAGAGTTTAGGAATCGGGTTTGAATTCAAACCGGTGAACCTAAATCTCACCGAACTAACTCGCGAGTCATTGAATTGTGACTCAGAGGATATTCTTGCTGTGAATTTCGCGTTTAATCTATACAAAATACTAGATGAGAGAGTGTCAACGGAGAATCCACGTGACACACTTCTCCGTCAAGTGAAATCACTCTCTCCAAGCATTGTCACAGTTTTGGAGCAAGAGATGAATACAAACACAACACCATTAGTTTCACGTGTGGCTGAAACTTGTTCTTATTACAGCACATTGCTAGAATCTATTGAATTTGCGATGGATCGTGATAGTTATGAGAGATTAAAGCTTGAAAAAGGACTGAGCATGAAGATGATAAATGCAGTTGCTTGTGAAGGGAGGGATCGTGTGGAAAGGTGTGAAGTTTTTGGGAAATGGCGTGCACGCATGAGCATGGCTGGGTTTAGGTTAAACCCGATGAGTCCGAAAGTTACTGAGTCAGTAACTTCAAGATTCATCCAAGGTAGCAGAATTACTGTGCATGAAGAGAATGGTGGAGTTTGCTTTGGTTGGATGGGAAAAACTCTCAATGTTGCATCTTCTTGGTGTTAA
Protein sequence

>MS.gene66114.t1

MSLRSNPNIQNPLPMNNSILQNANFSYENSDFAEIQSTVVPTSYDPDKKMMYELEKKLHEDIDDEEEWSLVVSGIMNTNNEWSETIPSEFSKQSLMEAATSIAEGKMDYAKEILFSFAHTQNPKLKFDSWLLDCIASALKLRPNNIENPPPVAELFSKEHAESTQLLFDHSLCFKLSFMAANIAILEAAFQDTTKSVKNLCVVDFDIGNGKQYINLLQELHTSLNGSPAMLKITIVTVNIHDDNLKSIGELLGREAESLGIGFEFKPVNLNLTELTRESLNCDSEDILAVNFAFNLYKILDERVSTENPRDTLLRQVKSLSPSIVTVLEQEMNTNTTPLVSRVAETCSYYSTLLESIEFAMDRDSYERLKLEKGLSMKMINAVACEGRDRVERCEVFGKWRARMSMAGFRLNPMSPKVTESVTSRFIQGSRITVHEENGGVCFGWMGKTLNVASSWC