AlfalfaGEDB | MS.gene66130

M. sativa cultivar XinJiangDaYe / MS.gene66130

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66130.t1	KEH15972.1	56.6	83	16	1	40	102	73	155	6.10E-10	73.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66130.t1	Q6S4P4	76.6	47	11	0	78	124	139	185	5.8e-09	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66130.t1	A0A072TF27	56.6	83	16	1	40	102	73	155	4.4e-10	73.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene66130.t1	TF	bZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66130.t1	MTR_0405s0060	55.952	84	17	1	39	102	72	155	2.53e-19	80.5
MS.gene66130.t1	MTR_4g072090	86.364	44	6	0	78	121	367	410	1.30e-16	75.1
MS.gene66130.t1	MTR_4g072090	86.364	44	6	0	78	121	367	410	1.44e-16	75.1
MS.gene66130.t1	MTR_2g099050	76.596	47	11	0	78	124	228	274	4.04e-15	70.9
MS.gene66130.t1	MTR_5g075390	69.811	53	16	0	78	130	166	218	3.14e-14	68.2
MS.gene66130.t1	MTR_3g467120	76.596	47	11	0	78	124	184	230	6.03e-14	67.4
MS.gene66130.t1	MTR_6g034945	74.468	47	12	0	78	124	158	204	1.22e-13	66.2
MS.gene66130.t1	MTR_5g024180	73.684	38	8	1	31	68	74	109	4.48e-12	59.7
MS.gene66130.t1	MTR_5g024180	73.684	38	8	1	31	68	37	72	4.51e-12	58.5
MS.gene66130.t1	MTR_3g094020	68.085	47	15	0	78	124	115	161	1.85e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66130.t1	AT4G38900	80.851	47	9	0	75	121	404	450	4.68e-16	73.6
MS.gene66130.t1	AT4G38900	84.091	44	7	0	78	121	401	444	9.95e-16	72.8
MS.gene66130.t1	AT4G38900	84.091	44	7	0	78	121	401	444	9.95e-16	72.8
MS.gene66130.t1	AT1G06850	75.000	48	12	0	78	125	155	202	1.59e-15	70.5
MS.gene66130.t1	AT1G43700	57.971	69	21	1	64	124	179	247	3.99e-15	70.5
MS.gene66130.t1	AT1G06070	76.596	47	11	0	78	124	217	263	8.15e-15	70.1
MS.gene66130.t1	AT1G06850	74.468	47	12	0	78	124	155	201	1.04e-13	66.6
MS.gene66130.t1	AT2G31370	69.811	53	16	0	78	130	208	260	1.83e-13	66.2
MS.gene66130.t1	AT2G31370	69.811	53	16	0	78	130	208	260	1.83e-13	66.2
MS.gene66130.t1	AT2G31370	69.811	53	16	0	78	130	208	260	1.83e-13	66.2

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Find 16 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCGATTATGTTTGCAG+GGG	0.668815	3.2:+47317563	None:intergenic
AAGTTGTGCAGAAAGTGTAG+TGG	0.665732	3.2:+47316621	None:intergenic
AATACCCCTGCAAACATAAT+CGG	0.426347	3.2:-47317567	MS.gene66130:CDS
ACAACTTACTTTGTTGCAGG+TGG	0.538284	3.2:-47316605	MS.gene66130:CDS
ACAATGCGTGCTTACAATAT+GGG	0.459061	3.2:-47317094	MS.gene66130:CDS
AGAAATCCGATTATGTTTGC+AGG	0.399917	3.2:+47317561	None:intergenic
ATAATCGGATTTCTGATGAA+TGG	0.444208	3.2:-47317552	MS.gene66130:CDS
CAACTTACTTTGTTGCAGGT+GGG	0.580671	3.2:-47316604	MS.gene66130:CDS
CATTTCGGAGCTAGAACAGA+AGG	0.562989	3.2:-47316665	MS.gene66130:CDS
GAAATCCGATTATGTTTGCA+GGG	0.552774	3.2:+47317562	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGAATGGAATGGATATAA+AGG	-	chr3.2:47317541-47317560	MS.gene66130:CDS	30.0%
ATAATCGGATTTCTGATGAA+TGG	-	chr3.2:47317277-47317296	MS.gene66130:intron	30.0%
ATTGAAAGAAGAACATGTCT+CGG	+	chr3.2:47316828-47316847	None:intergenic	30.0%
ATTTACGGTGAATTTGAACT+AGG	-	chr3.2:47317501-47317520	MS.gene66130:intron	30.0%
GTTAGTAATGTCAATATGGA+TGG	-	chr3.2:47318049-47318068	MS.gene66130:intron	30.0%
TACCTGAAAAACGTGATTAT+AGG	+	chr3.2:47317307-47317326	None:intergenic	30.0%
TTCAGGTAAGCAATTGTAAA+CGG	-	chr3.2:47317319-47317338	MS.gene66130:intron	30.0%
AAATCCGATTATGTTTGCAG+GGG	+	chr3.2:47317269-47317288	None:intergenic	35.0%
AATACCCCTGCAAACATAAT+CGG	-	chr3.2:47317262-47317281	MS.gene66130:intron	35.0%
AATTGGAACAAAGAGGAAAG+AGG	+	chr3.2:47316872-47316891	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	47316570	47318281	47316570	ID=MS.gene66130
chr3.2	mRNA	47316570	47318281	47316570	ID=MS.gene66130.t1;Parent=MS.gene66130
chr3.2	exon	47318253	47318281	47318253	ID=MS.gene66130.t1.exon1;Parent=MS.gene66130.t1
chr3.2	CDS	47318253	47318281	47318253	ID=cds.MS.gene66130.t1;Parent=MS.gene66130.t1
chr3.2	exon	47317528	47317618	47317528	ID=MS.gene66130.t1.exon2;Parent=MS.gene66130.t1
chr3.2	CDS	47317528	47317618	47317528	ID=cds.MS.gene66130.t1;Parent=MS.gene66130.t1
chr3.2	exon	47317061	47317170	47317061	ID=MS.gene66130.t1.exon3;Parent=MS.gene66130.t1
chr3.2	CDS	47317061	47317170	47317061	ID=cds.MS.gene66130.t1;Parent=MS.gene66130.t1
chr3.2	exon	47316570	47316741	47316570	ID=MS.gene66130.t1.exon4;Parent=MS.gene66130.t1
chr3.2	CDS	47316570	47316741	47316570	ID=cds.MS.gene66130.t1;Parent=MS.gene66130.t1

Gene Sequence

>MS.gene66130

ATGATGATTAACAATTTAGAGTGCCACATGTACTTTTACAATTTTTCAAGGCAAATCCTTATTATCTAATCCTAAGCTACAATGTAAACAAAACTTGCAAACCCTAATCCTAGCATTTGAGCGGGAAAAATGTGTTTACTAACTCATTTAATGTCAAATTCAAAATTCAAATCCCCTCCCACAATTTCTCACTAACTCTGCATTAATTGCACACGCGGTTTCATAAAGAATACAGATACAACTAATTGGTAATTACCGAGACATGTTCTTCTTTCAATATGCAACTTTTTGATTTCCAACCTCTTTCCTCTTTGTTCCAATTTTATTACCTATAATCTCTCTTCTTCCCACTCTCTCTCTCTTTTCTCTCTATATCTCTCTTACGAATCAGAAATCAACTGCACCATCACCCTATCGGAGCTCCGCCGTATCACCACCATCATATGTGGCAGCACCAGCTCTGACTATTTCCTCACAACCAAGCATTTCAGCTCCGCAGCTGCTGTCTTTGATTCCAACACAACACTTTCTTCTTGCTCAAATCAGAATCGCTGATCTTCACTCTCTTTTCTCTTCATCGAAGAGTCTTCCAATTCTGGGTTTTCACTTTTCAGCAGAAATCATCTTGAATCTTAATTCCTATCAATTGATTATTCAAATCAGATCTCAAATCGTTTCTGAGAAGAAACTAGAATACCCCTGCAAACATAATCGGATTTCTGATGAATGGCTTTCCTATAATCACGTTTTTCAGGTAAGCAATTGTAAACGGTTAATTTCTTGCTCACGCTTTCAACTTCTTCACTCTATTCTCACACCTCCATTATATGAAAATCGATTTACCGTTTTTGTGGTGAGTTTTGTGAAGCGATATAGTTTTCGTTGAATTTATTTTTAGGAAGTTTTGAATGTTATTGAGTTTTCTAACGTAATTTACGGTGAATTTGAACTAGGTTAGGAAAGTTGGTGGAAAGGAATGGAATGGATATAAAGGTTGAATTAGTTTAGGTTGCTGTGAAAATTTCACCTCTGTTTCATTGAAGAAAATTCATATAAATCTTAATTCGTGTTTTGATATTAAAATCGTTTTTGTGATTTATGTGTATTTTAGGATAAGGCTCCATTCATTGCTAAGGCTGCGAAGTTGAAAGAGGAGTATGAGAAGACAATGCGTGCTTACAATATGGGAATTGTAATTGCTTCTCTAACTTTTCTGTTACTGTGAATTTTTACTTCTCTTATGTTACTTGGATTTTTATTAACTTTTTGCTTATTTGAACTAATTTTGATGTCTTAGACTGAGAAGAATGCTTCGGAGGAAGAAGGATCTGATAAGTCTAAGTCTGAATTTGATGTCTTAGACTGAGAAGAATGCTTCGGAATTGAAGAAAATTATGGCTAATGAGAAAGTTGCTGAGATTGTTATGGCTGATCCGAAGCGGGCAAAGAGGTGTTGTAATTAACTTATTCAACGTTTTAGTTAGTAATGTCAATATGGATGGTTAATTTGACTTGGATATTTTTTTTTGGGTTATTTCAGGATTTTGAGAAACAGGCAATCGGCTGCGCTTTTCAATGAGCGCAAGATGCAATACATTTCGGAGCTAGAACAGAAGGTTCAGACTCTGCAGACTGAAGCCACTACACTTTCTGCACAACTTACTTTGTTGCAGGTGGGAACATTTATTTATGTTTGTGAAATCATGTTTTGA

Protein sequence

>MS.gene66130.t1

MMINNLECHISQIVSEKKLEYPCKHNRISDEWLSYNHVFQDKAPFIAKAAKLKEEYEKTMRAYNMGIVIASLTFLLLILRNRQSAALFNERKMQYISELEQKVQTLQTEATTLSAQLTLLQVGTFIYVCEIMF