Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66176.t1 | XP_003600264.2 | 95.4 | 283 | 11 | 1 | 1 | 283 | 1 | 281 | 2.90E-150 | 541.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66176.t1 | Q9SJ41 | 41.0 | 295 | 149 | 11 | 1 | 283 | 1 | 282 | 1.6e-45 | 184.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66176.t1 | G7J214 | 95.4 | 283 | 11 | 1 | 1 | 283 | 1 | 281 | 2.1e-150 | 541.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene66176.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66176.t1 | MTR_3g056160 | 95.406 | 283 | 11 | 1 | 1 | 283 | 1 | 281 | 0.0 | 564 |
| MS.gene66176.t1 | MTR_5g094700 | 56.678 | 307 | 105 | 9 | 1 | 283 | 1 | 303 | 1.88e-100 | 304 |
| MS.gene66176.t1 | MTR_0005s0130 | 26.512 | 215 | 120 | 4 | 1 | 184 | 1 | 208 | 3.15e-13 | 70.1 |
| MS.gene66176.t1 | MTR_4g043660 | 26.512 | 215 | 120 | 4 | 1 | 184 | 1 | 208 | 3.15e-13 | 70.1 |
| MS.gene66176.t1 | MTR_3g436060 | 33.333 | 138 | 85 | 1 | 1 | 131 | 1 | 138 | 3.59e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66176.t1 | AT3G52980 | 38.127 | 299 | 140 | 7 | 1 | 283 | 1 | 270 | 9.20e-59 | 192 |
| MS.gene66176.t1 | AT2G05160 | 40.000 | 295 | 152 | 10 | 1 | 283 | 1 | 282 | 1.39e-58 | 194 |
| MS.gene66176.t1 | AT2G05160 | 40.000 | 295 | 152 | 10 | 1 | 283 | 1 | 282 | 2.90e-58 | 195 |
| MS.gene66176.t1 | AT2G05160 | 40.000 | 295 | 152 | 10 | 1 | 283 | 1 | 282 | 2.90e-58 | 195 |
| MS.gene66176.t1 | AT3G51950 | 34.010 | 197 | 92 | 9 | 1 | 184 | 1 | 172 | 2.47e-19 | 88.2 |
| MS.gene66176.t1 | AT3G51950 | 34.010 | 197 | 92 | 9 | 1 | 184 | 1 | 172 | 2.47e-19 | 88.2 |
| MS.gene66176.t1 | AT3G51950 | 34.010 | 197 | 92 | 9 | 1 | 184 | 1 | 172 | 2.47e-19 | 88.2 |
| MS.gene66176.t1 | AT3G51950 | 34.010 | 197 | 92 | 9 | 1 | 184 | 1 | 172 | 2.47e-19 | 88.2 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.33e-13 | 70.1 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.45e-13 | 70.1 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.45e-13 | 70.1 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.51e-13 | 70.1 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.51e-13 | 70.1 |
| MS.gene66176.t1 | AT3G63450 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 2.51e-13 | 70.1 |
| MS.gene66176.t1 | AT3G21100 | 47.541 | 61 | 32 | 0 | 1 | 61 | 1 | 61 | 3.65e-11 | 63.9 |
| MS.gene66176.t1 | AT3G21100 | 47.541 | 61 | 32 | 0 | 1 | 61 | 1 | 61 | 3.65e-11 | 63.9 |
| MS.gene66176.t1 | AT3G21100 | 47.541 | 61 | 32 | 0 | 1 | 61 | 1 | 61 | 3.72e-11 | 63.9 |
| MS.gene66176.t1 | AT3G21100 | 47.541 | 61 | 32 | 0 | 1 | 61 | 1 | 61 | 3.72e-11 | 63.9 |
Find 50 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCCGGCGAAGAATTTC+CGG | 0.212833 | 3.3:+45826029 | None:intergenic |
| TGCTAAATTAGCTTCGTTTC+CGG | 0.258908 | 3.3:-45826127 | MS.gene66176:CDS |
| GGAAGTGAAGCAATTGAAAT+AGG | 0.268074 | 3.3:+45825553 | None:intergenic |
| TTCTTCGCCGGCAAGTATTC+AGG | 0.286968 | 3.3:-45826022 | MS.gene66176:CDS |
| GTGCCTATTGTCATAAATTC+TGG | 0.297480 | 3.3:+45825958 | None:intergenic |
| ATGATCTTTGTAGCATGTTC+TGG | 0.302836 | 3.3:+45826189 | None:intergenic |
| ACCATGATGCTGATTGTAAT+AGG | 0.338016 | 3.3:+45825990 | None:intergenic |
| ATACTATGATAAGTATAAAA+AGG | 0.338782 | 3.3:-45825524 | MS.gene66176:CDS |
| GATCCGTGAAGTTGCCCTTA+AGG | 0.356297 | 3.3:-45826097 | MS.gene66176:CDS |
| TCCGATTGCGAAATAGTATT+CGG | 0.386740 | 3.3:-45825426 | MS.gene66176:CDS |
| GACATAGTCTCTCATATGTA+TGG | 0.411648 | 3.3:-45825673 | MS.gene66176:CDS |
| ATTTCAGCAAAGGATATTGC+AGG | 0.419277 | 3.3:-45825741 | MS.gene66176:CDS |
| AGCCAACGACATGGCAAGTC+CGG | 0.430366 | 3.3:-45825472 | MS.gene66176:CDS |
| CCATGATGCTGATTGTAATA+GGG | 0.442845 | 3.3:+45825991 | None:intergenic |
| AGTCCAGAATTTATGACAAT+AGG | 0.443283 | 3.3:-45825961 | MS.gene66176:CDS |
| GCAGGCATGGAAATAGTTGT+AGG | 0.444946 | 3.3:-45825723 | MS.gene66176:CDS |
| TCTAGTTGCGCCAGTGATCC+AGG | 0.446585 | 3.3:+45825613 | None:intergenic |
| GCCGAATACTATTTCGCAAT+CGG | 0.448326 | 3.3:+45825425 | None:intergenic |
| GAACATGCTACAAAGATCAT+TGG | 0.453611 | 3.3:-45826186 | MS.gene66176:CDS |
| GGGTGAACCTGAATACTTGC+CGG | 0.454616 | 3.3:+45826015 | None:intergenic |
| AACGATGTCACCTGGATCAC+TGG | 0.458759 | 3.3:-45825623 | MS.gene66176:CDS |
| TTCAATTGCTTCACTTCCAA+TGG | 0.459864 | 3.3:-45825548 | MS.gene66176:CDS |
| ACAATGCAGTACGGGTTTGA+TGG | 0.469436 | 3.3:+45825929 | None:intergenic |
| TGGCCTTAAGGGCAACTTCA+CGG | 0.473570 | 3.3:+45826094 | None:intergenic |
| TCAGCTGTAGCCAATTTGAA+TGG | 0.476164 | 3.3:-45825820 | MS.gene66176:CDS |
| CAAAACTGAGCTTCAAAAGA+TGG | 0.480853 | 3.3:-45826073 | MS.gene66176:CDS |
| CAAACCCGTACTGCATTGTT+CGG | 0.489462 | 3.3:-45825925 | MS.gene66176:CDS |
| TACTTATCATAGTATGCCAT+TGG | 0.489665 | 3.3:+45825532 | None:intergenic |
| GCTCGCCGCTATCTTGCATA+AGG | 0.491407 | 3.3:+45826157 | None:intergenic |
| AGCAAAGGATATTGCAGGCA+TGG | 0.511519 | 3.3:-45825736 | MS.gene66176:CDS |
| AGCCGGACTTGCCATGTCGT+TGG | 0.516837 | 3.3:+45825470 | None:intergenic |
| CCCTATTACAATCAGCATCA+TGG | 0.517223 | 3.3:-45825991 | MS.gene66176:CDS |
| CCGTACTGCATTGTTCGGTT+CGG | 0.518157 | 3.3:-45825920 | MS.gene66176:CDS |
| CATGTCGTTGGCTCTCAGCT+AGG | 0.521057 | 3.3:+45825482 | None:intergenic |
| GTTGAGCTCTTAAAACAAAG+AGG | 0.521311 | 3.3:-45825580 | MS.gene66176:CDS |
| ACTTCACGGATCAAATTATC+CGG | 0.521367 | 3.3:+45826108 | None:intergenic |
| ACTAGATCAGTAATCTCGTC+CGG | 0.524588 | 3.3:-45826048 | MS.gene66176:CDS |
| ATAGTATTCGGCTAATTGAC+TGG | 0.532556 | 3.3:-45825414 | None:intergenic |
| AGTTGTAGGTTCTATCATCA+TGG | 0.533176 | 3.3:-45825709 | MS.gene66176:CDS |
| AAGCTTTGTCAAATTGTAGC+CGG | 0.544615 | 3.3:+45825453 | None:intergenic |
| CGAACCGAACAATGCAGTAC+GGG | 0.548958 | 3.3:+45825921 | None:intergenic |
| ATCACTGGCGCAACTAGAGT+CGG | 0.573776 | 3.3:-45825608 | MS.gene66176:CDS |
| CTCGTCCGGAAATTCTTCGC+CGG | 0.593097 | 3.3:-45826034 | MS.gene66176:CDS |
| ACATGTCACTATTTCAGCAA+AGG | 0.593665 | 3.3:-45825751 | MS.gene66176:CDS |
| CCGAACCGAACAATGCAGTA+CGG | 0.598627 | 3.3:+45825920 | None:intergenic |
| GATGAACAAACGATGTCACC+TGG | 0.614780 | 3.3:-45825631 | MS.gene66176:CDS |
| CTAGCTGAGAGCCAACGACA+TGG | 0.659391 | 3.3:-45825481 | MS.gene66176:CDS |
| TGATGCTGATTGTAATAGGG+AGG | 0.675488 | 3.3:+45825994 | None:intergenic |
| TATCTCCTTATGCAAGATAG+CGG | 0.677531 | 3.3:-45826162 | MS.gene66176:CDS |
| GATGCTGATTGTAATAGGGA+GGG | 0.712891 | 3.3:+45825995 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTATGATAAGTATAAAA+AGG | - | chr3.3:45826134-45826153 | MS.gene66176:CDS | 15.0% |
| !!! | AAATAGTGACATGTTTTGAA+TGG | + | chr3.3:45825901-45825920 | None:intergenic | 25.0% |
| AGTCCAGAATTTATGACAAT+AGG | - | chr3.3:45825697-45825716 | MS.gene66176:CDS | 30.0% | |
| GCCAATTTGAATGGAAAAAT+CGG | - | chr3.3:45825847-45825866 | MS.gene66176:CDS | 30.0% | |
| TACTTATCATAGTATGCCAT+TGG | + | chr3.3:45826129-45826148 | None:intergenic | 30.0% | |
| TGAATGGAAAAATCGGTAAA+AGG | - | chr3.3:45825854-45825873 | MS.gene66176:CDS | 30.0% | |
| !! | ACCGATTTTTCCATTCAAAT+TGG | + | chr3.3:45825851-45825870 | None:intergenic | 30.0% |
| ACATGTCACTATTTCAGCAA+AGG | - | chr3.3:45825907-45825926 | MS.gene66176:CDS | 35.0% | |
| ACTTCACGGATCAAATTATC+CGG | + | chr3.3:45825553-45825572 | None:intergenic | 35.0% | |
| AGTTGTAGGTTCTATCATCA+TGG | - | chr3.3:45825949-45825968 | MS.gene66176:CDS | 35.0% | |
| ATGATCTTTGTAGCATGTTC+TGG | + | chr3.3:45825472-45825491 | None:intergenic | 35.0% | |
| ATTTCAGCAAAGGATATTGC+AGG | - | chr3.3:45825917-45825936 | MS.gene66176:CDS | 35.0% | |
| CAAAACTGAGCTTCAAAAGA+TGG | - | chr3.3:45825585-45825604 | MS.gene66176:CDS | 35.0% | |
| GAACATGCTACAAAGATCAT+TGG | - | chr3.3:45825472-45825491 | MS.gene66176:CDS | 35.0% | |
| GACATAGTCTCTCATATGTA+TGG | - | chr3.3:45825985-45826004 | MS.gene66176:CDS | 35.0% | |
| GGAAGTGAAGCAATTGAAAT+AGG | + | chr3.3:45826108-45826127 | None:intergenic | 35.0% | |
| GTGCCTATTGTCATAAATTC+TGG | + | chr3.3:45825703-45825722 | None:intergenic | 35.0% | |
| GTTGAGCTCTTAAAACAAAG+AGG | - | chr3.3:45826078-45826097 | MS.gene66176:CDS | 35.0% | |
| TATCTCCTTATGCAAGATAG+CGG | - | chr3.3:45825496-45825515 | MS.gene66176:CDS | 35.0% | |
| TCCGATTGCGAAATAGTATT+CGG | - | chr3.3:45826232-45826251 | MS.gene66176:CDS | 35.0% | |
| TTCAATTGCTTCACTTCCAA+TGG | - | chr3.3:45826110-45826129 | MS.gene66176:CDS | 35.0% | |
| ! | AAGCTTTGTCAAATTGTAGC+CGG | + | chr3.3:45826208-45826227 | None:intergenic | 35.0% |
| ! | ACCATGATGCTGATTGTAAT+AGG | + | chr3.3:45825671-45825690 | None:intergenic | 35.0% |
| ! | CCATGATGCTGATTGTAATA+GGG | + | chr3.3:45825670-45825689 | None:intergenic | 35.0% |
| ! | TGCTAAATTAGCTTCGTTTC+CGG | - | chr3.3:45825531-45825550 | MS.gene66176:CDS | 35.0% |
| !! | AAAAAGGTGTTACAAGCTGA+AGG | - | chr3.3:45826150-45826169 | MS.gene66176:CDS | 35.0% |
| !!! | CATCTTTTGAAGCTCAGTTT+TGG | + | chr3.3:45825587-45825606 | None:intergenic | 35.0% |
| ACTAGATCAGTAATCTCGTC+CGG | - | chr3.3:45825610-45825629 | MS.gene66176:CDS | 40.0% | |
| CCCTATTACAATCAGCATCA+TGG | - | chr3.3:45825667-45825686 | MS.gene66176:CDS | 40.0% | |
| GCCGAATACTATTTCGCAAT+CGG | + | chr3.3:45826236-45826255 | None:intergenic | 40.0% | |
| TCAGCTGTAGCCAATTTGAA+TGG | - | chr3.3:45825838-45825857 | MS.gene66176:CDS | 40.0% | |
| ! | GATGCTGATTGTAATAGGGA+GGG | + | chr3.3:45825666-45825685 | None:intergenic | 40.0% |
| ! | TGATGCTGATTGTAATAGGG+AGG | + | chr3.3:45825667-45825686 | None:intergenic | 40.0% |
| !!! | AAGCTCAGTTTTGGCCTTAA+GGG | + | chr3.3:45825578-45825597 | None:intergenic | 40.0% |
| ACAATGCAGTACGGGTTTGA+TGG | + | chr3.3:45825732-45825751 | None:intergenic | 45.0% | |
| AGCAAAGGATATTGCAGGCA+TGG | - | chr3.3:45825922-45825941 | MS.gene66176:CDS | 45.0% | |
| CAAACCCGTACTGCATTGTT+CGG | - | chr3.3:45825733-45825752 | MS.gene66176:CDS | 45.0% | |
| GATGAACAAACGATGTCACC+TGG | - | chr3.3:45826027-45826046 | MS.gene66176:CDS | 45.0% | |
| GCAGGCATGGAAATAGTTGT+AGG | - | chr3.3:45825935-45825954 | MS.gene66176:CDS | 45.0% | |
| !!! | GAAGCTCAGTTTTGGCCTTA+AGG | + | chr3.3:45825579-45825598 | None:intergenic | 45.0% |
| AACGATGTCACCTGGATCAC+TGG | - | chr3.3:45826035-45826054 | MS.gene66176:CDS | 50.0% | |
| ACTTGCCGGCGAAGAATTTC+CGG | + | chr3.3:45825632-45825651 | None:intergenic | 50.0% | |
| ATCACTGGCGCAACTAGAGT+CGG | - | chr3.3:45826050-45826069 | MS.gene66176:CDS | 50.0% | |
| CCGAACCGAACAATGCAGTA+CGG | + | chr3.3:45825741-45825760 | None:intergenic | 50.0% | |
| CCGTACTGCATTGTTCGGTT+CGG | - | chr3.3:45825738-45825757 | MS.gene66176:CDS | 50.0% | |
| CGAACCGAACAATGCAGTAC+GGG | + | chr3.3:45825740-45825759 | None:intergenic | 50.0% | |
| GATCCGTGAAGTTGCCCTTA+AGG | - | chr3.3:45825561-45825580 | MS.gene66176:CDS | 50.0% | |
| GGGTGAACCTGAATACTTGC+CGG | + | chr3.3:45825646-45825665 | None:intergenic | 50.0% | |
| TTCTTCGCCGGCAAGTATTC+AGG | - | chr3.3:45825636-45825655 | MS.gene66176:CDS | 50.0% | |
| ! | TGGCCTTAAGGGCAACTTCA+CGG | + | chr3.3:45825567-45825586 | None:intergenic | 50.0% |
| AGCCAACGACATGGCAAGTC+CGG | - | chr3.3:45826186-45826205 | MS.gene66176:CDS | 55.0% | |
| CTAGCTGAGAGCCAACGACA+TGG | - | chr3.3:45826177-45826196 | MS.gene66176:CDS | 55.0% | |
| CTCGTCCGGAAATTCTTCGC+CGG | - | chr3.3:45825624-45825643 | MS.gene66176:CDS | 55.0% | |
| GCTCGCCGCTATCTTGCATA+AGG | + | chr3.3:45825504-45825523 | None:intergenic | 55.0% | |
| TCTAGTTGCGCCAGTGATCC+AGG | + | chr3.3:45826048-45826067 | None:intergenic | 55.0% | |
| !! | CATGTCGTTGGCTCTCAGCT+AGG | + | chr3.3:45826179-45826198 | None:intergenic | 55.0% |
| AGCCGGACTTGCCATGTCGT+TGG | + | chr3.3:45826191-45826210 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 45825415 | 45826265 | 45825415 | ID=MS.gene66176 |
| chr3.3 | mRNA | 45825415 | 45826265 | 45825415 | ID=MS.gene66176.t1;Parent=MS.gene66176 |
| chr3.3 | exon | 45825415 | 45826265 | 45825415 | ID=MS.gene66176.t1.exon1;Parent=MS.gene66176.t1 |
| chr3.3 | CDS | 45825415 | 45826265 | 45825415 | ID=cds.MS.gene66176.t1;Parent=MS.gene66176.t1 |
| Gene Sequence |
| Protein sequence |
