Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66327.t1 | XP_003620925.1 | 83.6 | 244 | 39 | 1 | 32 | 274 | 125 | 368 | 2.70E-113 | 418.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66327.t1 | F4K933 | 38.2 | 246 | 114 | 7 | 42 | 271 | 148 | 371 | 1.3e-34 | 148.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66327.t1 | G7KJT8 | 83.6 | 244 | 39 | 1 | 32 | 274 | 125 | 368 | 2.0e-113 | 418.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene66327.t1 | TF | HRT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66327.t1 | MTR_6g092520 | 83.607 | 244 | 39 | 1 | 32 | 274 | 125 | 368 | 1.50e-149 | 423 |
| MS.gene66327.t1 | MTR_7g090060 | 64.591 | 257 | 77 | 4 | 25 | 269 | 124 | 378 | 4.40e-109 | 320 |
| MS.gene66327.t1 | MTR_6g092480 | 28.947 | 152 | 76 | 3 | 22 | 172 | 3 | 123 | 7.94e-13 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66327.t1 | AT5G56780 | 38.211 | 246 | 114 | 7 | 42 | 271 | 148 | 371 | 1.19e-39 | 144 |
| MS.gene66327.t1 | AT4G26170 | 34.270 | 178 | 95 | 5 | 97 | 270 | 234 | 393 | 6.08e-20 | 89.4 |
| MS.gene66327.t1 | AT4G26170 | 31.333 | 150 | 62 | 2 | 159 | 267 | 355 | 504 | 1.09e-12 | 68.2 |
Find 40 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACATCATGTTTAATATTT+TGG | 0.103204 | 6.4:+63257116 | None:intergenic |
| GGCAACTTGTTTGATTTAAT+TGG | 0.184982 | 6.4:+63257409 | None:intergenic |
| TAAAATTTGTGGAGTGATTT+TGG | 0.186678 | 6.4:-63257257 | MS.gene66327:CDS |
| AATTCCACATATAATAGTTT+TGG | 0.286040 | 6.4:+63257045 | None:intergenic |
| TGTAGAAGACAACCAGTTAA+AGG | 0.297867 | 6.4:-63257001 | MS.gene66327:CDS |
| TGGTAGAAATGGAGCTCATT+TGG | 0.329770 | 6.4:-63258370 | None:intergenic |
| AGTGAAGGGTAGAAGTGATT+TGG | 0.349328 | 6.4:+63257450 | None:intergenic |
| GTTTGCAAGGCATCTCCCTT+TGG | 0.363238 | 6.4:+63257601 | None:intergenic |
| GAAACAATGACCGTCCCTTT+TGG | 0.364941 | 6.4:+63258316 | None:intergenic |
| TGAATTTGAACACGCGAGAT+AGG | 0.369579 | 6.4:+63257338 | None:intergenic |
| AAAAGATGTCATGAACATAA+AGG | 0.370970 | 6.4:-63256974 | MS.gene66327:CDS |
| GGTAGAAATGGAGCTCATTT+GGG | 0.380104 | 6.4:-63258369 | None:intergenic |
| ATTTGAATCTTCTCACCAAA+AGG | 0.382515 | 6.4:-63258331 | MS.gene66327:CDS |
| GATGTATCTTGAACTATCTT+AGG | 0.388616 | 6.4:+63257304 | None:intergenic |
| TTTCATGAGATCTTTCTTCA+AGG | 0.405599 | 6.4:-63258297 | MS.gene66327:CDS |
| AGACTCTCAGCCATTGTTTG+TGG | 0.408188 | 6.4:+63257085 | None:intergenic |
| ATGCTCAACAAAATCTCTTC+CGG | 0.410898 | 6.4:-63257496 | MS.gene66327:CDS |
| GAGAAGAATGGTAAAATTTG+TGG | 0.419383 | 6.4:-63257268 | MS.gene66327:CDS |
| AAAGATGTCATGAACATAAA+GGG | 0.444185 | 6.4:-63256973 | MS.gene66327:CDS |
| GAGCATTTCAAGAATATCAC+TGG | 0.446085 | 6.4:+63257513 | None:intergenic |
| AATGGAGCTCATTTGGGAAA+TGG | 0.456379 | 6.4:-63258363 | MS.gene66327:CDS |
| GTTAGGTGTCATGAACATAA+AGG | 0.469142 | 6.4:-63257193 | MS.gene66327:CDS |
| TGAGTACATTTGATTATGCT+TGG | 0.471865 | 6.4:-63257564 | MS.gene66327:CDS |
| CAGTTGAGATTCTGTTTGCA+AGG | 0.488213 | 6.4:+63257588 | None:intergenic |
| GCTTGGAACACAATTAATAA+CGG | 0.491690 | 6.4:-63257547 | MS.gene66327:CDS |
| ATCTGAAAATGTTCTTGTGC+CGG | 0.498102 | 6.4:+63257477 | None:intergenic |
| CCTTCACTCAAAAGAAAGTA+GGG | 0.503569 | 6.4:-63257435 | MS.gene66327:CDS |
| TTTGAATCTTCTCACCAAAA+GGG | 0.504759 | 6.4:-63258330 | MS.gene66327:CDS |
| CCCTTCACTCAAAAGAAAGT+AGG | 0.510670 | 6.4:-63257436 | MS.gene66327:CDS |
| AACTGGTTGTCTTCTACAAG+TGG | 0.517479 | 6.4:+63257006 | None:intergenic |
| GTCACCAAAACTATTATATG+TGG | 0.524845 | 6.4:-63257049 | MS.gene66327:CDS |
| TTGAACAGATGCAAACCAAA+GGG | 0.551932 | 6.4:-63257616 | MS.gene66327:CDS |
| GTTGAACAGATGCAAACCAA+AGG | 0.617085 | 6.4:-63257617 | MS.gene66327:CDS |
| TGAAGATCTTCCACAAACAA+TGG | 0.618416 | 6.4:-63257095 | MS.gene66327:CDS |
| AATCTTCTCACCAAAAGGGA+CGG | 0.621847 | 6.4:-63258326 | MS.gene66327:CDS |
| TTCTATTTGTACAAATACGC+CGG | 0.636888 | 6.4:-63257227 | MS.gene66327:CDS |
| AGAATATCACTGGGACGCCG+TGG | 0.641818 | 6.4:+63257523 | None:intergenic |
| ACACAATTAATAACGGTCCA+CGG | 0.642408 | 6.4:-63257540 | MS.gene66327:CDS |
| AGCATTTCAAGAATATCACT+GGG | 0.647075 | 6.4:+63257514 | None:intergenic |
| GAACTATCTTAGGACGCGAG+CGG | 0.662033 | 6.4:+63257314 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTTTGACTTTTTTTA+TGG | - | chr6.4:63257369-63257388 | MS.gene66327:CDS | 15.0% |
| !!! | GCATTTATATTTAATCTTTT+GGG | + | chr6.4:63257635-63257654 | None:intergenic | 15.0% |
| !!! | TAAAAATGATGATTAGTTTA+AGG | - | chr6.4:63257258-63257277 | MS.gene66327:CDS | 15.0% |
| !!! | TGCATTTATATTTAATCTTT+TGG | + | chr6.4:63257636-63257655 | None:intergenic | 15.0% |
| !!! | TTTTTCAATTGTTTATAGAT+GGG | - | chr6.4:63257028-63257047 | MS.gene66327:CDS | 15.0% |
| !! | ATAATATGTATCTCAAATCA+TGG | + | chr6.4:63257196-63257215 | None:intergenic | 20.0% |
| !! | TCAATTTATTAAGATCAAGA+TGG | - | chr6.4:63257090-63257109 | MS.gene66327:CDS | 20.0% |
| !! | TTAATTAAGAGCTAAGTTAT+AGG | - | chr6.4:63257457-63257476 | MS.gene66327:CDS | 20.0% |
| !!! | AATTCCACATATAATAGTTT+TGG | + | chr6.4:63258260-63258279 | None:intergenic | 20.0% |
| !!! | CAAAAAAAGTTACCTTTTAA+AGG | + | chr6.4:63257320-63257339 | None:intergenic | 20.0% |
| !!! | CAACATCATGTTTAATATTT+TGG | + | chr6.4:63258189-63258208 | None:intergenic | 20.0% |
| !!! | GTTTTTCAATTGTTTATAGA+TGG | - | chr6.4:63257027-63257046 | MS.gene66327:CDS | 20.0% |
| !!! | TATGAAGTAATTATTTTCTC+TGG | - | chr6.4:63257512-63257531 | MS.gene66327:CDS | 20.0% |
| !!! | TATGGAATAATTTCTTCTTT+TGG | - | chr6.4:63257387-63257406 | MS.gene66327:CDS | 20.0% |
| !!! | TTAGATTTTTTATCTTTCAC+AGG | + | chr6.4:63257917-63257936 | None:intergenic | 20.0% |
| !!! | TTAGCTCTTAATTAAACTAA+GGG | + | chr6.4:63257452-63257471 | None:intergenic | 20.0% |
| ! | AAAAGATGTCATGAACATAA+AGG | - | chr6.4:63258328-63258347 | MS.gene66327:CDS | 25.0% |
| ! | AAAGATGTCATGAACATAAA+GGG | - | chr6.4:63258329-63258348 | MS.gene66327:CDS | 25.0% |
| ! | CATATTTGCATCTTACATAA+AGG | - | chr6.4:63257654-63257673 | MS.gene66327:intron | 25.0% |
| ! | TTGCAAAATGAACATATACA+AGG | + | chr6.4:63257170-63257189 | None:intergenic | 25.0% |
| !! | AATTTTACCATTCTTCTCAA+TGG | + | chr6.4:63258032-63258051 | None:intergenic | 25.0% |
| !! | CTTAGCTCTTAATTAAACTA+AGG | + | chr6.4:63257453-63257472 | None:intergenic | 25.0% |
| !! | TAAAATTTGTGGAGTGATTT+TGG | - | chr6.4:63258045-63258064 | MS.gene66327:intron | 25.0% |
| !!! | TGTGGAATTATTTTGAAAGA+TGG | - | chr6.4:63258271-63258290 | MS.gene66327:CDS | 25.0% |
| AATGAACATATACAAGGTCA+AGG | + | chr6.4:63257164-63257183 | None:intergenic | 30.0% | |
| AATGCATAGAATGTACTTAC+AGG | + | chr6.4:63257056-63257075 | None:intergenic | 30.0% | |
| AGCATTTCAAGAATATCACT+GGG | + | chr6.4:63257791-63257810 | None:intergenic | 30.0% | |
| ATTTGAATCTTCTCACCAAA+AGG | - | chr6.4:63256971-63256990 | MS.gene66327:CDS | 30.0% | |
| GAGAAGAATGGTAAAATTTG+TGG | - | chr6.4:63258034-63258053 | MS.gene66327:intron | 30.0% | |
| GATGTATCTTGAACTATCTT+AGG | + | chr6.4:63258001-63258020 | None:intergenic | 30.0% | |
| GCTTGGAACACAATTAATAA+CGG | - | chr6.4:63257755-63257774 | MS.gene66327:intron | 30.0% | |
| GTCACCAAAACTATTATATG+TGG | - | chr6.4:63258253-63258272 | MS.gene66327:intron | 30.0% | |
| TAAGAGCTAAGTTATAGGTT+TGG | - | chr6.4:63257462-63257481 | MS.gene66327:CDS | 30.0% | |
| TGAGTACATTTGATTATGCT+TGG | - | chr6.4:63257738-63257757 | MS.gene66327:intron | 30.0% | |
| TTCTATTTGTACAAATACGC+CGG | - | chr6.4:63258075-63258094 | MS.gene66327:intron | 30.0% | |
| TTTCATGAGATCTTTCTTCA+AGG | - | chr6.4:63257005-63257024 | MS.gene66327:CDS | 30.0% | |
| TTTGAATCTTCTCACCAAAA+GGG | - | chr6.4:63256972-63256991 | MS.gene66327:CDS | 30.0% | |
| ! | CATCTTTTTCTTCCTTTAAC+TGG | + | chr6.4:63258316-63258335 | None:intergenic | 30.0% |
| ! | GATTTTTCCATTGAGAAGAA+TGG | - | chr6.4:63258022-63258041 | MS.gene66327:intron | 30.0% |
| ! | GGCAACTTGTTTGATTTAAT+TGG | + | chr6.4:63257896-63257915 | None:intergenic | 30.0% |
| !! | CACTCTTTAAGACCTTTAAA+AGG | - | chr6.4:63257305-63257324 | MS.gene66327:CDS | 30.0% |
| ACACAATTAATAACGGTCCA+CGG | - | chr6.4:63257762-63257781 | MS.gene66327:intron | 35.0% | |
| ATCTGAAAATGTTCTTGTGC+CGG | + | chr6.4:63257828-63257847 | None:intergenic | 35.0% | |
| ATGCTCAACAAAATCTCTTC+CGG | - | chr6.4:63257806-63257825 | MS.gene66327:intron | 35.0% | |
| CAAAAAGAGGAAATGCTTGT+AGG | - | chr6.4:63258161-63258180 | MS.gene66327:intron | 35.0% | |
| CCTTCACTCAAAAGAAAGTA+GGG | - | chr6.4:63257867-63257886 | MS.gene66327:intron | 35.0% | |
| GAGCATTTCAAGAATATCAC+TGG | + | chr6.4:63257792-63257811 | None:intergenic | 35.0% | |
| TCCAAGTCAGAATCAAAAAG+AGG | - | chr6.4:63258148-63258167 | MS.gene66327:intron | 35.0% | |
| TGAAGATCTTCCACAAACAA+TGG | - | chr6.4:63258207-63258226 | MS.gene66327:intron | 35.0% | |
| TGTAGAAGACAACCAGTTAA+AGG | - | chr6.4:63258301-63258320 | MS.gene66327:CDS | 35.0% | |
| TTGAACAGATGCAAACCAAA+GGG | - | chr6.4:63257686-63257705 | MS.gene66327:intron | 35.0% | |
| ! | GCAATGGTAATCTAGCATTT+TGG | + | chr6.4:63257142-63257161 | None:intergenic | 35.0% |
| ! | GTTAGGTGTCATGAACATAA+AGG | - | chr6.4:63258109-63258128 | MS.gene66327:intron | 35.0% |
| !! | CCTACTTTCTTTTGAGTGAA+GGG | + | chr6.4:63257869-63257888 | None:intergenic | 35.0% |
| !! | TCCTCTTTTTGATTCTGACT+TGG | + | chr6.4:63258152-63258171 | None:intergenic | 35.0% |
| AACTGGTTGTCTTCTACAAG+TGG | + | chr6.4:63258299-63258318 | None:intergenic | 40.0% | |
| AATCTTCTCACCAAAAGGGA+CGG | - | chr6.4:63256976-63256995 | MS.gene66327:CDS | 40.0% | |
| AGTGAAGGGTAGAAGTGATT+TGG | + | chr6.4:63257855-63257874 | None:intergenic | 40.0% | |
| CAGTTGAGATTCTGTTTGCA+AGG | + | chr6.4:63257717-63257736 | None:intergenic | 40.0% | |
| CATATACAAGGTCAAGGCAA+TGG | + | chr6.4:63257158-63257177 | None:intergenic | 40.0% | |
| CCCTTCACTCAAAAGAAAGT+AGG | - | chr6.4:63257866-63257885 | MS.gene66327:intron | 40.0% | |
| GTTGAACAGATGCAAACCAA+AGG | - | chr6.4:63257685-63257704 | MS.gene66327:intron | 40.0% | |
| TGAATTTGAACACGCGAGAT+AGG | + | chr6.4:63257967-63257986 | None:intergenic | 40.0% | |
| ! | TGTGGAGTGATTTTGGATGA+TGG | - | chr6.4:63258052-63258071 | MS.gene66327:intron | 40.0% |
| !! | AAAGGTCTTAAAGAGTGTCC+CGG | + | chr6.4:63257302-63257321 | None:intergenic | 40.0% |
| !! | CCCTACTTTCTTTTGAGTGA+AGG | + | chr6.4:63257870-63257889 | None:intergenic | 40.0% |
| !!! | CACCTAACTCTTTTTTCGAC+CGG | + | chr6.4:63258097-63258116 | None:intergenic | 40.0% |
| AAAATGTTAACGAGTGCCCC+GGG | - | chr6.4:63257281-63257300 | MS.gene66327:CDS | 45.0% | |
| AGACTCTCAGCCATTGTTTG+TGG | + | chr6.4:63258220-63258239 | None:intergenic | 45.0% | |
| GAAAATGTTAACGAGTGCCC+CGG | - | chr6.4:63257280-63257299 | MS.gene66327:CDS | 45.0% | |
| ! | GAAACAATGACCGTCCCTTT+TGG | + | chr6.4:63256989-63257008 | None:intergenic | 45.0% |
| !! | AAGGTCTTAAAGAGTGTCCC+GGG | + | chr6.4:63257301-63257320 | None:intergenic | 45.0% |
| CGCCGGTCGAAAAAAGAGTT+AGG | - | chr6.4:63258092-63258111 | MS.gene66327:intron | 50.0% | |
| GAACTATCTTAGGACGCGAG+CGG | + | chr6.4:63257991-63258010 | None:intergenic | 50.0% | |
| ! | GTTTGCAAGGCATCTCCCTT+TGG | + | chr6.4:63257704-63257723 | None:intergenic | 50.0% |
| !! | AATGCTTAAAGAGTGCCCCG+GGG | - | chr6.4:63257410-63257429 | MS.gene66327:CDS | 50.0% |
| !! | AGGTCTTAAAGAGTGTCCCG+GGG | + | chr6.4:63257300-63257319 | None:intergenic | 50.0% |
| !! | GAATGCTTAAAGAGTGCCCC+GGG | - | chr6.4:63257409-63257428 | MS.gene66327:CDS | 50.0% |
| !! | GGAATGCTTAAAGAGTGCCC+CGG | - | chr6.4:63257408-63257427 | MS.gene66327:CDS | 50.0% |
| AGAATATCACTGGGACGCCG+TGG | + | chr6.4:63257782-63257801 | None:intergenic | 55.0% | |
| TCTTGTTAACGAGTGCCCCC+GGG | + | chr6.4:63257429-63257448 | None:intergenic | 55.0% | |
| ! | GTCTTGTTAACGAGTGCCCC+CGG | + | chr6.4:63257430-63257449 | None:intergenic | 55.0% |
| !! | ATGCTTAAAGAGTGCCCCGG+GGG | - | chr6.4:63257411-63257430 | MS.gene66327:CDS | 55.0% |
| CTTGTTAACGAGTGCCCCCG+GGG | + | chr6.4:63257428-63257447 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 63256940 | 63258384 | 63256940 | ID=MS.gene66327 |
| chr6.4 | mRNA | 63256940 | 63258384 | 63256940 | ID=MS.gene66327.t1;Parent=MS.gene66327 |
| chr6.4 | exon | 63258269 | 63258384 | 63258269 | ID=MS.gene66327.t1.exon1;Parent=MS.gene66327.t1 |
| chr6.4 | CDS | 63258269 | 63258384 | 63258269 | ID=cds.MS.gene66327.t1;Parent=MS.gene66327.t1 |
| chr6.4 | exon | 63256940 | 63257648 | 63256940 | ID=MS.gene66327.t1.exon2;Parent=MS.gene66327.t1 |
| chr6.4 | CDS | 63256940 | 63257648 | 63256940 | ID=cds.MS.gene66327.t1;Parent=MS.gene66327.t1 |
| Gene Sequence |
| Protein sequence |
