AlfalfaGEDB | MS.gene66327

M. sativa cultivar XinJiangDaYe / MS.gene66327

KEGG: -
GO: -
PFAM: PF19239.2

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66327.t1	XP_003620925.1	83.6	244	39	1	32	274	125	368	2.70E-113	418.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66327.t1	F4K933	38.2	246	114	7	42	271	148	371	1.3e-34	148.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66327.t1	G7KJT8	83.6	244	39	1	32	274	125	368	2.0e-113	418.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene66327.t1	TF	HRT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66327.t1	MTR_6g092520	83.607	244	39	1	32	274	125	368	1.50e-149	423
MS.gene66327.t1	MTR_7g090060	64.591	257	77	4	25	269	124	378	4.40e-109	320
MS.gene66327.t1	MTR_6g092480	28.947	152	76	3	22	172	3	123	7.94e-13	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66327.t1	AT5G56780	38.211	246	114	7	42	271	148	371	1.19e-39	144
MS.gene66327.t1	AT4G26170	34.270	178	95	5	97	270	234	393	6.08e-20	89.4
MS.gene66327.t1	AT4G26170	31.333	150	62	2	159	267	355	504	1.09e-12	68.2

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Find 40 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGTCATGAACATAA+AGG	0.370970	6.4:-63256974	MS.gene66327:CDS
AAAGATGTCATGAACATAAA+GGG	0.444185	6.4:-63256973	MS.gene66327:CDS
AACTGGTTGTCTTCTACAAG+TGG	0.517479	6.4:+63257006	None:intergenic
AATCTTCTCACCAAAAGGGA+CGG	0.621847	6.4:-63258326	MS.gene66327:CDS
AATGGAGCTCATTTGGGAAA+TGG	0.456379	6.4:-63258363	MS.gene66327:CDS
AATTCCACATATAATAGTTT+TGG	0.286040	6.4:+63257045	None:intergenic
ACACAATTAATAACGGTCCA+CGG	0.642408	6.4:-63257540	MS.gene66327:CDS
AGAATATCACTGGGACGCCG+TGG	0.641818	6.4:+63257523	None:intergenic
AGACTCTCAGCCATTGTTTG+TGG	0.408188	6.4:+63257085	None:intergenic
AGCATTTCAAGAATATCACT+GGG	0.647075	6.4:+63257514	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAACATATACAAGGTCA+AGG	+	chr6.4:63257164-63257183	None:intergenic	30.0%
AATGCATAGAATGTACTTAC+AGG	+	chr6.4:63257056-63257075	None:intergenic	30.0%
AGCATTTCAAGAATATCACT+GGG	+	chr6.4:63257791-63257810	None:intergenic	30.0%
ATTTGAATCTTCTCACCAAA+AGG	-	chr6.4:63256971-63256990	MS.gene66327:CDS	30.0%
GAGAAGAATGGTAAAATTTG+TGG	-	chr6.4:63258034-63258053	MS.gene66327:intron	30.0%
GATGTATCTTGAACTATCTT+AGG	+	chr6.4:63258001-63258020	None:intergenic	30.0%
GCTTGGAACACAATTAATAA+CGG	-	chr6.4:63257755-63257774	MS.gene66327:intron	30.0%
GTCACCAAAACTATTATATG+TGG	-	chr6.4:63258253-63258272	MS.gene66327:intron	30.0%
TAAGAGCTAAGTTATAGGTT+TGG	-	chr6.4:63257462-63257481	MS.gene66327:CDS	30.0%
TGAGTACATTTGATTATGCT+TGG	-	chr6.4:63257738-63257757	MS.gene66327:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	63256940	63258384	63256940	ID=MS.gene66327
chr6.4	mRNA	63256940	63258384	63256940	ID=MS.gene66327.t1;Parent=MS.gene66327
chr6.4	exon	63258269	63258384	63258269	ID=MS.gene66327.t1.exon1;Parent=MS.gene66327.t1
chr6.4	CDS	63258269	63258384	63258269	ID=cds.MS.gene66327.t1;Parent=MS.gene66327.t1
chr6.4	exon	63256940	63257648	63256940	ID=MS.gene66327.t1.exon2;Parent=MS.gene66327.t1
chr6.4	CDS	63256940	63257648	63256940	ID=cds.MS.gene66327.t1;Parent=MS.gene66327.t1

Gene Sequence

>MS.gene66327

ATGGAGCTCATTTGGGAAATGGCTGTTCTTCATTTGAATCTTCTCACCAAAAGGGACGGTCATTGTTTCATGAGATCTTTCTTCAAGGTTTTTCAATTGTTTATAGATGGGCTCCTGTAAGTACATTCTATGCATTTACATTATTTTTCTTCAATTTATTAAGATCAAGATGGTTTCAATTGAACATGTATTGATTGATCCAAAATGCTAGATTACCATTGCCTTGACCTTGTATATGTTCATTTTGCAAAATCCATGATTTGAGATACATATTATTCTATGATAGTATAATTGAAGATGATTAGTGAATACTTGATTTAAAAATGATGATTAGTTTAAGGAAAATGTTAACGAGTGCCCCGGGACACTCTTTAAGACCTTTAAAAGGTAACTTTTTTTGAAAAGTAATGTAATCAATGCATTGAAATTGAAATTTTTGACTTTTTTTATGGAATAATTTCTTCTTTTGGAATGCTTAAAGAGTGCCCCGGGGGCACTCGTTAACAAGACCCTTAGTTTAATTAAGAGCTAAGTTATAGGTTTGGAAGTCTAGAATGAATTAATTGAGTGATTATGAAGTAATTATTTTCTCTGGTTGTGAGTTAATCAATTAAGTCTTTGTAATCAAATATTTACTATGATGATTGTGAGAATAATTAGTAATCTACACAATTTGTGTGAAGCGTTGTTGACCCAAAAGATTAAATATAAATGCATATTTGCATCTTACATAAAGGATTCATTTGTTGAACAGATGCAAACCAAAGGGAGATGCCTTGCAAACAGAATCTCAACTGTTGAGTACATTTGATTATGCTTGGAACACAATTAATAACGGTCCACGGCGTCCCAGTGATATTCTTGAAATGCTCAACAAAATCTCTTCCGGCACAAGAACATTTTCAGATGTAGCCAAATCACTTCTACCCTTCACTCAAAAGAAAGTAGGGATACCAATTAAATCAAACAAGTTGCCTGTGAAAGATAAAAAATCTAATGAAGCAGATAATGACAGCTACAATTTCCTATCTCGCGTGTTCAAATTCAACCGCTCGCGTCCTAAGATAGTTCAAGATACATCTGATTTTTCCATTGAGAAGAATGGTAAAATTTGTGGAGTGATTTTGGATGATGGTTCTATTTGTACAAATACGCCGGTCGAAAAAAGAGTTAGGTGTCATGAACATAAAGGAATGAGAATAAGAACATCCAAGTCAGAATCAAAAAGAGGAAATGCTTGTAGGTACCAAAATATTAAACATGATGTTGAAGATCTTCCACAAACAATGGCTGAGAGTCTTGTTGTTGAGAGTGTCACCAAAACTATTATATGTGGAATTATTTTGAAAGATGGATCCACTTGTAGAAGACAACCAGTTAAAGGAAGAAAAAGATGTCATGAACATAAAGGGAAGAGAATTCGTGCATTTGTTCCTATAAATTAG

Protein sequence

>MS.gene66327.t1

MELIWEMAVLHLNLLTKRDGHCFMRSFFKVFQLFIDGLLIHLLNRCKPKGDALQTESQLLSTFDYAWNTINNGPRRPSDILEMLNKISSGTRTFSDVAKSLLPFTQKKVGIPIKSNKLPVKDKKSNEADNDSYNFLSRVFKFNRSRPKIVQDTSDFSIEKNGKICGVILDDGSICTNTPVEKRVRCHEHKGMRIRTSKSESKRGNACRYQNIKHDVEDLPQTMAESLVVESVTKTIICGIILKDGSTCRRQPVKGRKRCHEHKGKRIRAFVPIN