AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66369

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66369.t1 MTR_6g090380 95.526 380 14 2 1 380 1 377 0.0 749
MS.gene66369.t1 MTR_8g102100 58.191 409 107 10 1 379 1 375 2.66e-149 427
MS.gene66369.t1 MTR_3g103960 48.220 309 101 13 76 379 23 277 2.50e-81 250
MS.gene66369.t1 MTR_4g078660 69.355 124 36 1 86 207 40 163 1.22e-61 201
MS.gene66369.t1 MTR_2g090605 78.846 104 22 0 104 207 77 180 3.14e-61 202
MS.gene66369.t1 MTR_7g092070 55.556 180 64 3 85 252 245 420 3.33e-58 201
MS.gene66369.t1 MTR_3g029590 52.514 179 74 4 85 252 245 423 7.00e-56 195
MS.gene66369.t1 MTR_5g082830 45.333 225 98 5 85 287 198 419 1.34e-53 188
MS.gene66369.t1 MTR_5g071070 43.534 232 93 6 77 275 114 340 2.35e-53 187
MS.gene66369.t1 MTR_5g071070 43.534 232 93 6 77 275 196 422 8.05e-53 186
MS.gene66369.t1 MTR_1g075890 68.224 107 34 0 100 206 225 331 2.06e-50 179
MS.gene66369.t1 MTR_1g050438 64.486 107 38 0 100 206 224 330 3.15e-49 175
MS.gene66369.t1 MTR_0728s0020 64.486 107 38 0 100 206 240 346 6.57e-49 174
MS.gene66369.t1 MTR_3g435680 60.656 122 46 1 85 206 134 253 6.77e-49 170
MS.gene66369.t1 MTR_3g070880 50.345 145 63 2 99 243 158 293 3.68e-47 168
MS.gene66369.t1 MTR_3g435590 48.447 161 74 3 100 254 234 391 4.93e-46 164
MS.gene66369.t1 MTR_3g464270 47.468 158 70 6 103 251 8 161 1.13e-38 142
MS.gene66369.t1 MTR_3g464260 52.000 125 50 2 135 252 212 333 3.86e-34 132
MS.gene66369.t1 MTR_3g464290 56.790 81 34 1 98 177 94 174 1.39e-28 116
MS.gene66369.t1 MTR_3g464280 31.140 228 107 5 98 317 182 367 7.86e-23 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66369.t1 AT4G29190 51.629 399 119 11 4 379 8 355 2.25e-122 358
MS.gene66369.t1 AT2G19810 50.765 392 131 10 6 379 11 358 6.04e-115 339
MS.gene66369.t1 AT2G25900 45.763 354 139 8 4 352 9 314 2.90e-89 272
MS.gene66369.t1 AT2G25900 45.763 354 139 8 4 352 9 314 2.90e-89 272
MS.gene66369.t1 AT1G03790 73.874 111 28 1 98 207 76 186 1.10e-58 196
MS.gene66369.t1 AT2G41900 65.414 133 43 1 74 206 234 363 2.23e-58 202
MS.gene66369.t1 AT5G44260 72.973 111 29 1 98 207 54 164 2.57e-57 192
MS.gene66369.t1 AT5G12850 51.934 181 80 1 79 252 232 412 1.61e-56 196
MS.gene66369.t1 AT5G58620 51.955 179 71 3 85 251 190 365 4.90e-56 194
MS.gene66369.t1 AT2G40140 52.571 175 76 3 87 260 197 365 1.53e-52 184
MS.gene66369.t1 AT2G40140 52.571 175 76 3 87 260 197 365 1.53e-52 184
MS.gene66369.t1 AT3G55980 51.381 181 71 4 87 260 194 364 7.27e-52 182
MS.gene66369.t1 AT3G55980 51.381 181 71 4 87 260 261 431 1.70e-51 182
MS.gene66369.t1 AT5G07500 58.955 134 53 1 73 206 23 154 7.60e-50 168

Find 116 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTCTTTCTTCTTATTT+TGG 0.118905 6.1:+78854880 MS.gene66369:CDS
GGGTTGGTGGGTTTGATTTA+TGG 0.119649 6.1:+78855748 MS.gene66369:CDS
CTTTCAACTCTCTCCATTAT+AGG 0.177073 6.1:-78855792 None:intergenic
TTGAAAGTGGAAGAGATATT+AGG 0.228511 6.1:+78855808 MS.gene66369:CDS
ATAGTAACATTCCTATTAAT+TGG 0.241559 6.1:-78855621 None:intergenic
TGGGAATTCGGGTTTGGGTT+TGG 0.258433 6.1:+78855881 MS.gene66369:CDS
TGGGAATGGGAATTCGGGTT+TGG 0.271735 6.1:+78855875 MS.gene66369:CDS
GGGAATGGGAATTCGGGTTT+GGG 0.276394 6.1:+78855876 MS.gene66369:CDS
GGTTGGTGGGTTTGATTTAT+GGG 0.285810 6.1:+78855749 MS.gene66369:CDS
GAGAATGGGAATGGGAATTC+GGG 0.298840 6.1:+78855870 MS.gene66369:CDS
GGAGAATGGGAATGGGAATT+CGG 0.309885 6.1:+78855869 MS.gene66369:CDS
CCGGGTTTAGGCCGGGTTGG+TGG 0.326895 6.1:+78855735 MS.gene66369:CDS
TGAAAACTATGCCAATTAAT+AGG 0.328736 6.1:+78855610 MS.gene66369:CDS
TCCGGCATCGGCGGCGGCGG+CGG 0.332930 6.1:+78855071 MS.gene66369:CDS
TCCACCGGTGATTACTCTTC+CGG 0.334046 6.1:+78854967 MS.gene66369:CDS
ATTAATAGGAATGTTACTAT+TGG 0.347132 6.1:+78855624 MS.gene66369:CDS
GTCGTTTCGTAATTTACAAT+TGG 0.353057 6.1:+78855581 MS.gene66369:CDS
TGAAAGTGGAAGAGATATTA+GGG 0.354237 6.1:+78855809 MS.gene66369:CDS
CAGTTTCCTTTACGAAAATC+AGG 0.366299 6.1:-78855264 None:intergenic
CGGTTCTCCGTTACGACAAA+TGG 0.366550 6.1:+78855491 MS.gene66369:CDS
TCGGGTTTGGGTTTGGATGC+GGG 0.376728 6.1:+78855888 MS.gene66369:CDS
TTCGGGTTTGGGTTTGGATG+CGG 0.380726 6.1:+78855887 MS.gene66369:CDS
TCGTTTCGTAATTTACAATT+GGG 0.384486 6.1:+78855582 MS.gene66369:CDS
AAGAAAATTCATTGGAGAAT+GGG 0.400460 6.1:+78855856 MS.gene66369:CDS
ATTAAGCAAAGAAAATTCAT+TGG 0.406900 6.1:+78855848 MS.gene66369:CDS
GAAGCGCTGCAAGTGCTTCT+CGG 0.409877 6.1:-78855002 None:intergenic
ATCGGCGGCGAAGGAGATTC+TGG 0.412112 6.1:-78855531 None:intergenic
ATCTCCGGCATCGGCGGCGG+CGG 0.417722 6.1:+78855068 MS.gene66369:CDS
GTCAACCCGGTCCTGATGTT+GGG 0.417880 6.1:+78855910 MS.gene66369:CDS
TTGTGAAGAGGAGCCTATAA+TGG 0.419641 6.1:+78855779 MS.gene66369:CDS
CGGCGGCGTGCTTTCTCGCC+GGG 0.433922 6.1:-78855204 None:intergenic
GCCGTTTGATCATCATGATT+CGG 0.436898 6.1:-78854856 None:intergenic
CGATTCTGATTTAGAATCTC+CGG 0.438051 6.1:+78855053 MS.gene66369:CDS
GGTCAACCCGGTCCTGATGT+TGG 0.438292 6.1:+78855909 MS.gene66369:CDS
GAATCGTTGAACTCGTTTGA+CGG 0.440489 6.1:-78855036 None:intergenic
AAAGAAAATTCATTGGAGAA+TGG 0.444951 6.1:+78855855 MS.gene66369:CDS
CGTAAAGGAAACTGTAAGAA+AGG 0.457732 6.1:+78855273 MS.gene66369:CDS
GGAGAACCGTCATACGAATC+TGG 0.463298 6.1:-78855477 None:intergenic
CAACGGTTCATGTTCCTCCT+TGG 0.464865 6.1:+78854833 MS.gene66369:CDS
AGAATCTCCGGCATCGGCGG+CGG 0.471287 6.1:+78855065 MS.gene66369:CDS
CGGGTTTAGGCCGGGTTGGT+GGG 0.471920 6.1:+78855736 MS.gene66369:CDS
ACGGCGGCGTGCTTTCTCGC+CGG 0.477321 6.1:-78855205 None:intergenic
ATCGGTGACATCGGCGGCGA+AGG 0.483470 6.1:-78855540 None:intergenic
CGTCTTTACACGGCTGAGTA+CGG 0.485485 6.1:-78855353 None:intergenic
AATTCATTGGAGAATGGGAA+TGG 0.486755 6.1:+78855861 MS.gene66369:CDS
GGCGGCGGCGGCGGATTCTA+CGG 0.488265 6.1:+78855080 MS.gene66369:CDS
GCAAACACGGCGGCGACAGC+TGG 0.489963 6.1:-78855379 None:intergenic
TCACAAACCCACCCAACATC+AGG 0.492003 6.1:-78855921 None:intergenic
ATTCATTGGAGAATGGGAAT+GGG 0.494528 6.1:+78855862 MS.gene66369:CDS
GAATGTTACTATTGGTTCAC+CGG 0.498343 6.1:+78855632 MS.gene66369:CDS
TTCTCGCCGGGATGTGCGTA+TGG 0.499904 6.1:-78855192 None:intergenic
TTTGGGTCACCGCTTCGGTC+CGG 0.502096 6.1:+78855678 MS.gene66369:CDS
AACCCACCCAACATCAGGAC+CGG 0.507115 6.1:-78855916 None:intergenic
GCCGAATCATGATGATCAAA+CGG 0.508151 6.1:+78854855 MS.gene66369:CDS
ACCCGGTCCTGATGTTGGGT+GGG 0.513166 6.1:+78855914 MS.gene66369:CDS
ACGTTAAGACGGAGCTGCTC+CGG 0.516425 6.1:-78855417 None:intergenic
CCACCAACCCGGCCTAAACC+CGG 0.518833 6.1:-78855735 None:intergenic
AGCAGAGTCCATCTTCCTCT+CGG 0.519878 6.1:+78855442 MS.gene66369:CDS
AACCCGGTCCTGATGTTGGG+TGG 0.522130 6.1:+78855913 MS.gene66369:CDS
GCCGGCGATCTCACCGGCGC+CGG 0.528291 6.1:+78854922 MS.gene66369:CDS
GGTGAACCAGAACCGAAAAC+CGG 0.530214 6.1:-78855651 None:intergenic
TTAACACTCCGAGAGGAAGA+TGG 0.530641 6.1:-78855450 None:intergenic
AAAATCAGGACAAGCCGTAC+CGG 0.530716 6.1:-78855250 None:intergenic
CTGCAAGTGCTTCTCGGAGA+TGG 0.532581 6.1:-78854996 None:intergenic
AACAATGCCGGCGATCTCAC+CGG 0.537849 6.1:+78854916 MS.gene66369:CDS
GGTTTGGATGCGGGTCAACC+CGG 0.549221 6.1:+78855897 MS.gene66369:CDS
TGATTTAGAATCTCCGGCAT+CGG 0.549413 6.1:+78855059 MS.gene66369:CDS
AATGAAACTTCCTCGGATCA+CGG 0.551799 6.1:-78855224 None:intergenic
AACTCTCCAGATTCGTATGA+CGG 0.556291 6.1:+78855471 MS.gene66369:CDS
CGTACTCAGCCGTGTAAAGA+CGG 0.559308 6.1:+78855354 MS.gene66369:CDS
TACGATAACAATAACAATGC+CGG 0.559578 6.1:+78854904 MS.gene66369:CDS
AAACCGGGTTTAGGCCGGGT+TGG 0.560051 6.1:+78855732 MS.gene66369:CDS
TCGTTCGCCATCGGTGACAT+CGG 0.561352 6.1:-78855549 None:intergenic
GAAGTTTCATTACTCCGGTA+CGG 0.561534 6.1:+78855236 MS.gene66369:CDS
CAGCTGGTTCCGTCTTTACA+CGG 0.564199 6.1:-78855363 None:intergenic
TCCGGCGCCGGTGAGATCGC+CGG 0.564683 6.1:-78854923 None:intergenic
GTCACCATCTCGTTCGCCAT+CGG 0.566053 6.1:-78855558 None:intergenic
TTCGTAATTTACAATTGGGG+AGG 0.566121 6.1:+78855586 MS.gene66369:CDS
GGCTGAGTACGGTAACGAGC+AGG 0.567252 6.1:-78855342 None:intergenic
CCGAGGAAGTTTCATTACTC+CGG 0.569117 6.1:+78855231 MS.gene66369:CDS
ATCTCCGGTAGTGTAATCTC+CGG 0.569727 6.1:-78854941 None:intergenic
CACGTGGAAGATCACATGAC+TGG 0.574790 6.1:+78855160 MS.gene66369:CDS
GGTAGTGTAATCTCCGGCGC+CGG 0.576624 6.1:-78854935 None:intergenic
TTCGCCATCGGTGACATCGG+CGG 0.580138 6.1:-78855546 None:intergenic
GACTCTGCTGAACGTTAAGA+CGG 0.581166 6.1:-78855428 None:intergenic
TGATCATCATGATTCGGCCA+AGG 0.582191 6.1:-78854850 None:intergenic
CGTTTCGTAATTTACAATTG+GGG 0.584271 6.1:+78855583 MS.gene66369:CDS
TTCGCCGCCGATGTCACCGA+TGG 0.587560 6.1:+78855542 MS.gene66369:CDS
GGCGCCGGAGATTACACTAC+CGG 0.590957 6.1:+78854937 MS.gene66369:CDS
TTCTCGGAGATGGTAATCAC+CGG 0.596124 6.1:-78854986 None:intergenic
AAGCACGCCGCCGTGATCCG+AGG 0.597183 6.1:+78855214 MS.gene66369:CDS
ATAGTAACCATTTGTCGTAA+CGG 0.598472 6.1:-78855498 None:intergenic
CCGGAGTAATGAAACTTCCT+CGG 0.604275 6.1:-78855231 None:intergenic
ATGGGATCAGAGTTGTGAAG+AGG 0.606158 6.1:+78855767 MS.gene66369:CDS
TTGCTTCTTCGCTCACACGC+CGG 0.608383 6.1:+78855398 MS.gene66369:CDS
GTCACCGATGGCGAACGAGA+TGG 0.608528 6.1:+78855554 MS.gene66369:CDS
GATTGTCCATACGCACATCC+CGG 0.608961 6.1:+78855186 MS.gene66369:CDS
TTTAGAATCTCCGGCATCGG+CGG 0.621860 6.1:+78855062 MS.gene66369:CDS
TTATCTTGAACTCGTACATG+CGG 0.628647 6.1:-78855128 None:intergenic
TGGAAGATCACATGACTGGA+CGG 0.631350 6.1:+78855164 MS.gene66369:CDS
ATAATGGAGAGAGTTGAAAG+TGG 0.632760 6.1:+78855795 MS.gene66369:CDS
CAAGGAGGAACATGAACCGT+TGG 0.633061 6.1:-78854832 None:intergenic
ATAAATCAAACCCACCAACC+CGG 0.643239 6.1:-78855746 None:intergenic
GACGCGTGTGAACACGCGCA+TGG 0.649749 6.1:+78855297 MS.gene66369:CDS
ACCGGAAGAGTAATCACCGG+TGG 0.651463 6.1:-78854968 None:intergenic
ACCCACCCAACATCAGGACC+GGG 0.651526 6.1:-78855915 None:intergenic
AAGATAAGAAGATGCGCACG+TGG 0.652747 6.1:+78855144 MS.gene66369:CDS
ATCACCGGAAGAGTAATCAC+CGG 0.655530 6.1:-78854971 None:intergenic
TGTGAGCGAAGAAGCAAACA+CGG 0.674890 6.1:-78855392 None:intergenic
GGCGGCGAAGGAGATTCTGG+TGG 0.678659 6.1:-78855528 None:intergenic
TCATCATGATTCGGCCAAGG+AGG 0.681712 6.1:-78854847 None:intergenic
GGCGGCGGCGGATTCTACGG+TGG 0.691096 6.1:+78855083 MS.gene66369:CDS
TGGAGAGTTAACACTCCGAG+AGG 0.702069 6.1:-78855457 None:intergenic
GATGCTGAGAGCTAATCCAA+CGG 0.723355 6.1:+78854816 MS.gene66369:CDS
GAAACTTCCTCGGATCACGG+CGG 0.765354 6.1:-78855221 None:intergenic
GAGCGAAGAAGCAAACACGG+CGG 0.843910 6.1:-78855389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAACATTCCTATTAAT+TGG - chr6.1:78855624-78855643 None:intergenic 20.0%
!! ATTAAGCAAAGAAAATTCAT+TGG + chr6.1:78855848-78855867 MS.gene66369:CDS 20.0%
!! ATTAATAGGAATGTTACTAT+TGG + chr6.1:78855624-78855643 MS.gene66369:CDS 20.0%
! AAAGAAAATTCATTGGAGAA+TGG + chr6.1:78855855-78855874 MS.gene66369:CDS 25.0%
! AAGAAAATTCATTGGAGAAT+GGG + chr6.1:78855856-78855875 MS.gene66369:CDS 25.0%
! TCGTTTCGTAATTTACAATT+GGG + chr6.1:78855582-78855601 MS.gene66369:CDS 25.0%
! TGAAAACTATGCCAATTAAT+AGG + chr6.1:78855610-78855629 MS.gene66369:CDS 25.0%
!!! GAGATTCTTTCTTCTTATTT+TGG + chr6.1:78854880-78854899 MS.gene66369:CDS 25.0%
ATAGTAACCATTTGTCGTAA+CGG - chr6.1:78855501-78855520 None:intergenic 30.0%
ATTGGGCAAACTTAAAAAAC+CGG - chr6.1:78855700-78855719 None:intergenic 30.0%
CGTTTCGTAATTTACAATTG+GGG + chr6.1:78855583-78855602 MS.gene66369:CDS 30.0%
GTCGTTTCGTAATTTACAAT+TGG + chr6.1:78855581-78855600 MS.gene66369:CDS 30.0%
TACGATAACAATAACAATGC+CGG + chr6.1:78854904-78854923 MS.gene66369:CDS 30.0%
TGAAAGTGGAAGAGATATTA+GGG + chr6.1:78855809-78855828 MS.gene66369:CDS 30.0%
TTGAAAGTGGAAGAGATATT+AGG + chr6.1:78855808-78855827 MS.gene66369:CDS 30.0%
AATACCCCAACTAAAAAACC+GGG + chr6.1:78855717-78855736 MS.gene66369:CDS 35.0%
AATTCATTGGAGAATGGGAA+TGG + chr6.1:78855861-78855880 MS.gene66369:CDS 35.0%
ATTCATTGGAGAATGGGAAT+GGG + chr6.1:78855862-78855881 MS.gene66369:CDS 35.0%
CAATACCCCAACTAAAAAAC+CGG + chr6.1:78855716-78855735 MS.gene66369:CDS 35.0%
CAGTTTCCTTTACGAAAATC+AGG - chr6.1:78855267-78855286 None:intergenic 35.0%
CGTAAAGGAAACTGTAAGAA+AGG + chr6.1:78855273-78855292 MS.gene66369:CDS 35.0%
CTTTCAACTCTCTCCATTAT+AGG - chr6.1:78855795-78855814 None:intergenic 35.0%
TTATCTTGAACTCGTACATG+CGG - chr6.1:78855131-78855150 None:intergenic 35.0%
TTCGTAATTTACAATTGGGG+AGG + chr6.1:78855586-78855605 MS.gene66369:CDS 35.0%
! ATAATGGAGAGAGTTGAAAG+TGG + chr6.1:78855795-78855814 MS.gene66369:CDS 35.0%
! CGATTCTGATTTAGAATCTC+CGG + chr6.1:78855053-78855072 MS.gene66369:CDS 35.0%
!! GAATGTTACTATTGGTTCAC+CGG + chr6.1:78855632-78855651 MS.gene66369:CDS 35.0%
!!! CTAAACCCGGTTTTTTAGTT+GGG - chr6.1:78855725-78855744 None:intergenic 35.0%
!!! GGTTTTTTAGTTGGGGTATT+GGG - chr6.1:78855717-78855736 None:intergenic 35.0%
!!! TAAACCCGGTTTTTTAGTTG+GGG - chr6.1:78855724-78855743 None:intergenic 35.0%
AACTCTCCAGATTCGTATGA+CGG + chr6.1:78855471-78855490 MS.gene66369:CDS 40.0%
AATGAAACTTCCTCGGATCA+CGG - chr6.1:78855227-78855246 None:intergenic 40.0%
ATAAATCAAACCCACCAACC+CGG - chr6.1:78855749-78855768 None:intergenic 40.0%
CCAACTAAAAAACCGGGTTT+AGG + chr6.1:78855723-78855742 MS.gene66369:CDS 40.0%
GAAGTTTCATTACTCCGGTA+CGG + chr6.1:78855236-78855255 MS.gene66369:CDS 40.0%
GAATCGTTGAACTCGTTTGA+CGG - chr6.1:78855039-78855058 None:intergenic 40.0%
GCCGAATCATGATGATCAAA+CGG + chr6.1:78854855-78854874 MS.gene66369:CDS 40.0%
GCCGTTTGATCATCATGATT+CGG - chr6.1:78854859-78854878 None:intergenic 40.0%
TTGTGAAGAGGAGCCTATAA+TGG + chr6.1:78855779-78855798 MS.gene66369:CDS 40.0%
! GCTTGTCCTGATTTTCGTAA+AGG + chr6.1:78855258-78855277 MS.gene66369:CDS 40.0%
! TGATTTAGAATCTCCGGCAT+CGG + chr6.1:78855059-78855078 MS.gene66369:CDS 40.0%
!! GGTTGGTGGGTTTGATTTAT+GGG + chr6.1:78855749-78855768 MS.gene66369:CDS 40.0%
!!! ACTATTGGTTCACCGGTTTT+CGG + chr6.1:78855639-78855658 MS.gene66369:CDS 40.0%
!!! CCTAAACCCGGTTTTTTAGT+TGG - chr6.1:78855726-78855745 None:intergenic 40.0%
!!! CGGTTTTTTAGTTGGGGTAT+TGG - chr6.1:78855718-78855737 None:intergenic 40.0%
AAAATCAGGACAAGCCGTAC+CGG - chr6.1:78855253-78855272 None:intergenic 45.0%
AAGATAAGAAGATGCGCACG+TGG + chr6.1:78855144-78855163 MS.gene66369:CDS 45.0%
ATCACCGGAAGAGTAATCAC+CGG - chr6.1:78854974-78854993 None:intergenic 45.0%
ATCACCGGTGGAAAAATCTC+CGG - chr6.1:78854959-78854978 None:intergenic 45.0%
ATCTCCGGTAGTGTAATCTC+CGG - chr6.1:78854944-78854963 None:intergenic 45.0%
ATGGGATCAGAGTTGTGAAG+AGG + chr6.1:78855767-78855786 MS.gene66369:CDS 45.0%
CCGAGGAAGTTTCATTACTC+CGG + chr6.1:78855231-78855250 MS.gene66369:CDS 45.0%
CCGGAGTAATGAAACTTCCT+CGG - chr6.1:78855234-78855253 None:intergenic 45.0%
CTAAAAAACCGGGTTTAGGC+CGG + chr6.1:78855727-78855746 MS.gene66369:CDS 45.0%
CTTAAAAAACCGGACCGAAG+CGG - chr6.1:78855690-78855709 None:intergenic 45.0%
GACTCTGCTGAACGTTAAGA+CGG - chr6.1:78855431-78855450 None:intergenic 45.0%
GAGAATGGGAATGGGAATTC+GGG + chr6.1:78855870-78855889 MS.gene66369:CDS 45.0%
GATGCTGAGAGCTAATCCAA+CGG + chr6.1:78854816-78854835 MS.gene66369:CDS 45.0%
GGAGAATGGGAATGGGAATT+CGG + chr6.1:78855869-78855888 MS.gene66369:CDS 45.0%
TAAAAAACCGGGTTTAGGCC+GGG + chr6.1:78855728-78855747 MS.gene66369:CDS 45.0%
TGGAAGATCACATGACTGGA+CGG + chr6.1:78855164-78855183 MS.gene66369:CDS 45.0%
TGTGAGCGAAGAAGCAAACA+CGG - chr6.1:78855395-78855414 None:intergenic 45.0%
TTAACACTCCGAGAGGAAGA+TGG - chr6.1:78855453-78855472 None:intergenic 45.0%
TTCTCGGAGATGGTAATCAC+CGG - chr6.1:78854989-78855008 None:intergenic 45.0%
! ACTACCGGAGATTTTTCCAC+CGG + chr6.1:78854952-78854971 MS.gene66369:CDS 45.0%
! TGATCATCATGATTCGGCCA+AGG - chr6.1:78854853-78854872 None:intergenic 45.0%
!! GGGTTGGTGGGTTTGATTTA+TGG + chr6.1:78855748-78855767 MS.gene66369:CDS 45.0%
!!! GTTCTGGTTCACCGGTTTTT+GGG + chr6.1:78855661-78855680 MS.gene66369:CDS 45.0%
AGCAGAGTCCATCTTCCTCT+CGG + chr6.1:78855442-78855461 MS.gene66369:CDS 50.0%
CAACGGTTCATGTTCCTCCT+TGG + chr6.1:78854833-78854852 MS.gene66369:CDS 50.0%
CAAGGAGGAACATGAACCGT+TGG - chr6.1:78854835-78854854 None:intergenic 50.0%
CACGTGGAAGATCACATGAC+TGG + chr6.1:78855160-78855179 MS.gene66369:CDS 50.0%
CGGTTCTCCGTTACGACAAA+TGG + chr6.1:78855491-78855510 MS.gene66369:CDS 50.0%
CGTACTCAGCCGTGTAAAGA+CGG + chr6.1:78855354-78855373 MS.gene66369:CDS 50.0%
CGTCTTTACACGGCTGAGTA+CGG - chr6.1:78855356-78855375 None:intergenic 50.0%
GATTGTCCATACGCACATCC+CGG + chr6.1:78855186-78855205 MS.gene66369:CDS 50.0%
GGAGAACCGTCATACGAATC+TGG - chr6.1:78855480-78855499 None:intergenic 50.0%
GGTGAACCAGAACCGAAAAC+CGG - chr6.1:78855654-78855673 None:intergenic 50.0%
TCACAAACCCACCCAACATC+AGG - chr6.1:78855924-78855943 None:intergenic 50.0%
TCCACCGGTGATTACTCTTC+CGG + chr6.1:78854967-78854986 MS.gene66369:CDS 50.0%
TGGAGAGTTAACACTCCGAG+AGG - chr6.1:78855460-78855479 None:intergenic 50.0%
! CAGCTGGTTCCGTCTTTACA+CGG - chr6.1:78855366-78855385 None:intergenic 50.0%
! GGGAATGGGAATTCGGGTTT+GGG + chr6.1:78855876-78855895 MS.gene66369:CDS 50.0%
! TCATCATGATTCGGCCAAGG+AGG - chr6.1:78854850-78854869 None:intergenic 50.0%
! TGGGAATGGGAATTCGGGTT+TGG + chr6.1:78855875-78855894 MS.gene66369:CDS 50.0%
! TGGGAATTCGGGTTTGGGTT+TGG + chr6.1:78855881-78855900 MS.gene66369:CDS 50.0%
! TTCGGGTTTGGGTTTGGATG+CGG + chr6.1:78855887-78855906 MS.gene66369:CDS 50.0%
! TTTAGAATCTCCGGCATCGG+CGG + chr6.1:78855062-78855081 MS.gene66369:CDS 50.0%
!!! CGCATGGTGTTTTCGAGTGT+TGG + chr6.1:78855313-78855332 MS.gene66369:CDS 50.0%
!!! GGTTCTGGTTCACCGGTTTT+TGG + chr6.1:78855660-78855679 MS.gene66369:CDS 50.0%
!!! GGTTTTCGGTTCTGGTTCAC+CGG + chr6.1:78855653-78855672 MS.gene66369:CDS 50.0%
AACAATGCCGGCGATCTCAC+CGG + chr6.1:78854916-78854935 MS.gene66369:CDS 55.0%
AACCCACCCAACATCAGGAC+CGG - chr6.1:78855919-78855938 None:intergenic 55.0%
ACCGGAAGAGTAATCACCGG+TGG - chr6.1:78854971-78854990 None:intergenic 55.0%
ACGTTAAGACGGAGCTGCTC+CGG - chr6.1:78855420-78855439 None:intergenic 55.0%
CGAAGCGGTGACCCAAAAAC+CGG - chr6.1:78855675-78855694 None:intergenic 55.0%
CTGCAAGTGCTTCTCGGAGA+TGG - chr6.1:78854999-78855018 None:intergenic 55.0%
GAAACTTCCTCGGATCACGG+CGG - chr6.1:78855224-78855243 None:intergenic 55.0%
GAGCGAAGAAGCAAACACGG+CGG - chr6.1:78855392-78855411 None:intergenic 55.0%
GTCAACCCGGTCCTGATGTT+GGG + chr6.1:78855910-78855929 MS.gene66369:CDS 55.0%
GTCACCATCTCGTTCGCCAT+CGG - chr6.1:78855561-78855580 None:intergenic 55.0%
TCGTTCGCCATCGGTGACAT+CGG - chr6.1:78855552-78855571 None:intergenic 55.0%
TTGCTTCTTCGCTCACACGC+CGG + chr6.1:78855398-78855417 MS.gene66369:CDS 55.0%
! TCGGGTTTGGGTTTGGATGC+GGG + chr6.1:78855888-78855907 MS.gene66369:CDS 55.0%
!! GAAGCGCTGCAAGTGCTTCT+CGG - chr6.1:78855005-78855024 None:intergenic 55.0%
!!! CGGTTTTTGGGTCACCGCTT+CGG + chr6.1:78855673-78855692 MS.gene66369:CDS 55.0%
!!! GGTTCACCGGTTTTCGGTTC+TGG + chr6.1:78855645-78855664 MS.gene66369:CDS 55.0%
AAACCGGGTTTAGGCCGGGT+TGG + chr6.1:78855732-78855751 MS.gene66369:CDS 60.0%
AACCCGGTCCTGATGTTGGG+TGG + chr6.1:78855913-78855932 MS.gene66369:CDS 60.0%
ACCCACCCAACATCAGGACC+GGG - chr6.1:78855918-78855937 None:intergenic 60.0%
ACCCGGTCCTGATGTTGGGT+GGG + chr6.1:78855914-78855933 MS.gene66369:CDS 60.0%
ATCGGCGGCGAAGGAGATTC+TGG - chr6.1:78855534-78855553 None:intergenic 60.0%
GGCTGAGTACGGTAACGAGC+AGG - chr6.1:78855345-78855364 None:intergenic 60.0%
GGTAGTGTAATCTCCGGCGC+CGG - chr6.1:78854938-78854957 None:intergenic 60.0%
GGTCAACCCGGTCCTGATGT+TGG + chr6.1:78855909-78855928 MS.gene66369:CDS 60.0%
GGTTTGGATGCGGGTCAACC+CGG + chr6.1:78855897-78855916 MS.gene66369:CDS 60.0%
GTCACCGATGGCGAACGAGA+TGG + chr6.1:78855554-78855573 MS.gene66369:CDS 60.0%
TTCGCCATCGGTGACATCGG+CGG - chr6.1:78855549-78855568 None:intergenic 60.0%
TTCTCGCCGGGATGTGCGTA+TGG - chr6.1:78855195-78855214 None:intergenic 60.0%
TTTGGGTCACCGCTTCGGTC+CGG + chr6.1:78855678-78855697 MS.gene66369:CDS 60.0%
! GGCGCCGGAGATTACACTAC+CGG + chr6.1:78854937-78854956 MS.gene66369:CDS 60.0%
ATCGGTGACATCGGCGGCGA+AGG - chr6.1:78855543-78855562 None:intergenic 65.0%
CCACCAACCCGGCCTAAACC+CGG - chr6.1:78855738-78855757 None:intergenic 65.0%
GACGCGTGTGAACACGCGCA+TGG + chr6.1:78855297-78855316 MS.gene66369:CDS 65.0%
GGCGGCGAAGGAGATTCTGG+TGG - chr6.1:78855531-78855550 None:intergenic 65.0%
TTCGCCGCCGATGTCACCGA+TGG + chr6.1:78855542-78855561 MS.gene66369:CDS 65.0%
!! AGAATCTCCGGCATCGGCGG+CGG + chr6.1:78855065-78855084 MS.gene66369:CDS 65.0%
!! CGGGTTTAGGCCGGGTTGGT+GGG + chr6.1:78855736-78855755 MS.gene66369:CDS 65.0%
AAGCACGCCGCCGTGATCCG+AGG + chr6.1:78855214-78855233 MS.gene66369:CDS 70.0%
ACGGCGGCGTGCTTTCTCGC+CGG - chr6.1:78855208-78855227 None:intergenic 70.0%
GCAAACACGGCGGCGACAGC+TGG - chr6.1:78855382-78855401 None:intergenic 70.0%
! CCGGGTTTAGGCCGGGTTGG+TGG + chr6.1:78855735-78855754 MS.gene66369:CDS 70.0%
CGGCGGCGTGCTTTCTCGCC+GGG - chr6.1:78855207-78855226 None:intergenic 75.0%
! GGCGGCGGCGGATTCTACGG+TGG + chr6.1:78855083-78855102 MS.gene66369:CDS 75.0%
! GGCGGCGGCGGCGGATTCTA+CGG + chr6.1:78855080-78855099 MS.gene66369:CDS 75.0%
!! ATCTCCGGCATCGGCGGCGG+CGG + chr6.1:78855068-78855087 MS.gene66369:CDS 75.0%
!! TCCGGCGCCGGTGAGATCGC+CGG - chr6.1:78854926-78854945 None:intergenic 75.0%
! GCCGGCGATCTCACCGGCGC+CGG + chr6.1:78854922-78854941 MS.gene66369:CDS 80.0%
! TCCGCCGCCGCCGCCGATGC+CGG - chr6.1:78855075-78855094 None:intergenic 85.0%
!!! TCCGGCATCGGCGGCGGCGG+CGG + chr6.1:78855071-78855090 MS.gene66369:CDS 85.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 78854814 78855956 78854814 ID=MS.gene66369
chr6.1 mRNA 78854814 78855956 78854814 ID=MS.gene66369.t1;Parent=MS.gene66369
chr6.1 exon 78854814 78855956 78854814 ID=MS.gene66369.t1.exon1;Parent=MS.gene66369.t1
chr6.1 CDS 78854814 78855956 78854814 ID=cds.MS.gene66369.t1;Parent=MS.gene66369.t1
Gene Sequence

>MS.gene66369

ATGATGCTGAGAGCTAATCCAACGGTTCATGTTCCTCCTTGGCCGAATCATGATGATCAAACGGCTGAGATTCTTTCTTCTTATTTTGGTTACGATAACAATAACAATGCCGGCGATCTCACCGGCGCCGGAGATTACACTACCGGAGATTTTTCCACCGGTGATTACTCTTCCGGTGATTACCATCTCCGAGAAGCACTTGCAGCGCTTCAACGTTATCTTCCGTCAAACGAGTTCAACGATTCTGATTTAGAATCTCCGGCATCGGCGGCGGCGGCGGATTCTACGGTGGATGCATATTCATGCGATCATTTCCGCATGTACGAGTTCAAGATAAGAAGATGCGCACGTGGAAGATCACATGACTGGACGGATTGTCCATACGCACATCCCGGCGAGAAAGCACGCCGCCGTGATCCGAGGAAGTTTCATTACTCCGGTACGGCTTGTCCTGATTTTCGTAAAGGAAACTGTAAGAAAGGTGACGCGTGTGAACACGCGCATGGTGTTTTCGAGTGTTGGCTTCATCCTGCTCGTTACCGTACTCAGCCGTGTAAAGACGGAACCAGCTGTCGCCGCCGTGTTTGCTTCTTCGCTCACACGCCGGAGCAGCTCCGTCTTAACGTTCAGCAGAGTCCATCTTCCTCTCGGAGTGTTAACTCTCCAGATTCGTATGACGGTTCTCCGTTACGACAAATGGTTACTATAACGACGCCACCAGAATCTCCTTCGCCGCCGATGTCACCGATGGCGAACGAGATGGTGACGTCGTTTCGTAATTTACAATTGGGGAGGATGAAAACTATGCCAATTAATAGGAATGTTACTATTGGTTCACCGGTTTTCGGTTCTGGTTCACCGGTTTTTGGGTCACCGCTTCGGTCCGGTTTTTTAAGTTTGCCCAATACCCCAACTAAAAAACCGGGTTTAGGCCGGGTTGGTGGGTTTGATTTATGGGATCAGAGTTGTGAAGAGGAGCCTATAATGGAGAGAGTTGAAAGTGGAAGAGATATTAGGGCTAAGATGTTTGAGAAATTAAGCAAAGAAAATTCATTGGAGAATGGGAATGGGAATTCGGGTTTGGGTTTGGATGCGGGTCAACCCGGTCCTGATGTTGGGTGGGTTTGTGATTTGTTGAAGTGA
Protein sequence

>MS.gene66369.t1

MMLRANPTVHVPPWPNHDDQTAEILSSYFGYDNNNNAGDLTGAGDYTTGDFSTGDYSSGDYHLREALAALQRYLPSNEFNDSDLESPASAAAADSTVDAYSCDHFRMYEFKIRRCARGRSHDWTDCPYAHPGEKARRRDPRKFHYSGTACPDFRKGNCKKGDACEHAHGVFECWLHPARYRTQPCKDGTSCRRRVCFFAHTPEQLRLNVQQSPSSSRSVNSPDSYDGSPLRQMVTITTPPESPSPPMSPMANEMVTSFRNLQLGRMKTMPINRNVTIGSPVFGSGSPVFGSPLRSGFLSLPNTPTKKPGLGRVGGFDLWDQSCEEEPIMERVESGRDIRAKMFEKLSKENSLENGNGNSGLGLDAGQPGPDVGWVCDLLK