AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66598

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66598.t1 MTR_4g049790 73.132 629 103 5 1 629 1 563 0.0 827
MS.gene66598.t1 MTR_4g047580 67.276 602 135 8 28 629 1 540 0.0 730
MS.gene66598.t1 MTR_4g049760 70.070 568 117 5 33 600 8 522 0.0 701
MS.gene66598.t1 MTR_2g088000 41.667 612 232 23 36 606 64 591 6.49e-109 341
MS.gene66598.t1 MTR_2g023930 36.746 547 235 15 60 578 67 530 1.03e-82 271
MS.gene66598.t1 MTR_4g132430 56.579 228 85 6 344 565 105 324 1.87e-72 238
MS.gene66598.t1 MTR_4g132430 56.579 228 85 6 344 565 239 458 1.31e-71 240
MS.gene66598.t1 MTR_3g053330 47.479 238 95 5 346 577 231 444 9.42e-63 216
MS.gene66598.t1 MTR_7g110720 45.736 258 96 7 351 602 174 393 1.47e-56 197
MS.gene66598.t1 MTR_7g110720 45.736 258 96 7 351 602 201 420 2.34e-56 197
MS.gene66598.t1 MTR_2g045360 48.039 204 91 5 351 542 196 396 9.25e-47 171
MS.gene66598.t1 MTR_7g009730 51.613 124 54 1 323 440 170 293 3.78e-37 147
MS.gene66598.t1 MTR_4g094412 37.079 267 136 6 351 592 228 487 2.16e-35 140
MS.gene66598.t1 MTR_4g094412 37.079 267 136 6 351 592 219 478 3.64e-35 139
MS.gene66598.t1 MTR_4g107970 45.550 191 73 5 327 486 121 311 8.39e-33 133
MS.gene66598.t1 MTR_4g107970 45.550 191 73 5 327 486 198 388 1.16e-32 134
MS.gene66598.t1 MTR_3g056100 44.037 109 57 2 339 444 231 338 7.79e-25 108
MS.gene66598.t1 MTR_3g056100 44.037 109 57 2 339 444 244 351 9.07e-25 108
MS.gene66598.t1 MTR_3g056100 44.037 109 57 2 339 444 375 482 1.24e-24 108
MS.gene66598.t1 MTR_3g056100 44.037 109 57 2 339 444 388 495 1.34e-24 108
MS.gene66598.t1 MTR_5g042920 50.515 97 43 2 336 428 286 381 2.05e-24 107
MS.gene66598.t1 MTR_5g042920 36.111 108 65 3 367 474 152 255 6.39e-11 65.5
MS.gene66598.t1 MTR_7g011820 48.454 97 49 1 342 438 386 481 3.90e-24 107
MS.gene66598.t1 MTR_5g094430 34.911 169 75 4 349 514 213 349 3.95e-23 102
MS.gene66598.t1 MTR_5g074400 48.387 93 47 1 336 428 390 481 4.47e-23 104
MS.gene66598.t1 MTR_4g007060 34.437 151 75 4 343 493 105 231 4.97e-23 100
MS.gene66598.t1 MTR_5g094430 34.911 169 75 4 349 514 235 371 5.16e-23 102
MS.gene66598.t1 MTR_4g082580 51.852 81 38 1 348 428 260 339 7.73e-23 101
MS.gene66598.t1 MTR_4g007060 34.437 151 75 4 343 493 139 265 8.52e-23 100
MS.gene66598.t1 MTR_3g009470 54.321 81 36 1 348 428 477 556 1.24e-22 103
MS.gene66598.t1 MTR_3g009470 43.750 64 34 2 367 430 281 342 4.29e-11 66.6
MS.gene66598.t1 MTR_3g031220 46.316 95 50 1 333 427 332 425 2.09e-22 101
MS.gene66598.t1 MTR_3g031220 40.230 87 46 3 344 430 180 260 4.29e-12 69.3
MS.gene66598.t1 MTR_5g094430 34.911 169 75 4 349 514 347 483 2.82e-22 101
MS.gene66598.t1 MTR_7g071120 54.321 81 36 1 348 428 306 385 5.41e-22 100
MS.gene66598.t1 MTR_4g013260 53.165 79 36 1 348 426 318 395 8.60e-22 99.8
MS.gene66598.t1 MTR_4g013260 40.698 86 44 3 350 433 170 250 2.37e-11 67.0
MS.gene66598.t1 MTR_7g080000 42.609 115 63 2 316 428 380 493 1.13e-21 100
MS.gene66598.t1 MTR_4g094588 41.667 108 62 1 353 460 165 271 3.48e-21 95.9
MS.gene66598.t1 MTR_5g044695 66.038 53 18 0 344 396 55 107 3.75e-21 89.4
MS.gene66598.t1 MTR_7g109800 45.745 94 46 2 351 444 130 218 4.20e-21 94.7
MS.gene66598.t1 MTR_7g080020 50.617 81 39 1 348 428 475 554 4.21e-21 98.6
MS.gene66598.t1 MTR_3g106060 41.837 98 56 1 327 423 98 195 6.67e-21 94.0
MS.gene66598.t1 MTR_5g097490 51.807 83 38 2 347 428 232 313 6.97e-21 95.9
MS.gene66598.t1 MTR_2g105060 50.000 78 37 1 352 427 153 230 1.06e-20 94.0
MS.gene66598.t1 MTR_2g105060 50.000 78 37 1 352 427 78 155 1.12e-20 92.0
MS.gene66598.t1 MTR_1g013790 50.000 66 33 0 361 426 96 161 1.56e-20 92.0
MS.gene66598.t1 MTR_1g013760 44.565 92 51 0 351 442 125 216 3.08e-20 92.0
MS.gene66598.t1 MTR_6g038890 66.038 53 18 0 344 396 166 218 4.63e-20 89.7
MS.gene66598.t1 MTR_1g015140 52.703 74 34 1 351 424 87 159 1.28e-19 86.7
MS.gene66598.t1 MTR_8g096730 37.736 106 65 1 324 429 28 132 1.41e-19 86.3
MS.gene66598.t1 MTR_1g009613 56.716 67 29 0 367 433 263 329 1.66e-19 90.9
MS.gene66598.t1 MTR_7g100510 42.857 98 45 2 329 426 334 420 2.14e-19 91.7
MS.gene66598.t1 MTR_7g100510 44.444 72 38 2 367 438 190 259 1.85e-11 67.0
MS.gene66598.t1 MTR_2g033820 47.500 80 41 1 351 430 188 266 2.45e-19 90.9
MS.gene66598.t1 MTR_7g079010 45.263 95 48 2 353 447 128 218 3.32e-19 87.4
MS.gene66598.t1 MTR_3g104750 40.187 107 56 2 318 424 63 161 5.09e-19 85.1
MS.gene66598.t1 MTR_4g094908 56.452 62 27 0 367 428 267 328 5.20e-19 89.4
MS.gene66598.t1 MTR_2g032030 31.792 173 98 5 283 436 166 337 5.21e-19 90.9
MS.gene66598.t1 MTR_2g032030 31.792 173 98 5 283 436 166 337 6.97e-19 90.1
MS.gene66598.t1 MTR_1g086790 44.444 90 45 2 353 442 145 229 1.03e-18 88.2
MS.gene66598.t1 MTR_7g028415 52.857 70 32 1 356 425 83 151 1.77e-18 84.0
MS.gene66598.t1 MTR_7g028710 52.857 70 32 1 356 425 83 151 1.77e-18 84.0
MS.gene66598.t1 MTR_8g087000 53.968 63 29 0 366 428 77 139 1.83e-18 85.9
MS.gene66598.t1 MTR_8g087000 53.968 63 29 0 366 428 76 138 1.85e-18 85.9
MS.gene66598.t1 MTR_5g016610 56.667 60 26 0 366 425 199 258 2.14e-18 86.3
MS.gene66598.t1 MTR_7g109600 42.857 91 49 1 362 452 49 136 3.01e-18 85.5
MS.gene66598.t1 MTR_2g024350 34.752 141 80 3 361 490 3 142 3.58e-18 84.3
MS.gene66598.t1 MTR_1g070455 57.895 57 24 0 367 423 294 350 6.81e-18 86.3
MS.gene66598.t1 MTR_2g083870 46.835 79 42 0 357 435 71 149 7.00e-18 84.3
MS.gene66598.t1 MTR_5g091390 47.436 78 40 1 353 430 144 220 1.13e-17 84.7
MS.gene66598.t1 MTR_4g130900 35.669 157 82 4 291 430 268 422 1.43e-17 86.7
MS.gene66598.t1 MTR_2g016770 51.562 64 31 0 363 426 32 95 2.49e-17 81.6
MS.gene66598.t1 MTR_4g122530 47.368 76 39 1 352 427 194 268 3.56e-17 84.7
MS.gene66598.t1 MTR_2g016770 51.562 64 31 0 363 426 24 87 3.59e-17 80.9
MS.gene66598.t1 MTR_8g007270 48.101 79 40 1 345 423 133 210 4.58e-17 81.3
MS.gene66598.t1 MTR_7g079040 48.000 75 39 0 362 436 150 224 5.88e-17 82.8
MS.gene66598.t1 MTR_2g024380 44.186 86 46 1 352 437 239 322 1.22e-16 83.6
MS.gene66598.t1 MTR_3g095080 41.667 96 54 2 349 444 127 220 1.53e-16 79.7
MS.gene66598.t1 MTR_3g095080 43.478 92 50 2 353 444 155 244 2.18e-16 79.7
MS.gene66598.t1 MTR_8g028565 46.667 75 40 0 362 436 151 225 2.29e-16 81.3
MS.gene66598.t1 MTR_4g127040 53.448 58 27 0 366 423 245 302 3.99e-16 80.1
MS.gene66598.t1 MTR_8g092010 44.156 77 42 1 353 429 95 170 4.31e-16 77.4
MS.gene66598.t1 MTR_8g098945 44.444 72 40 0 365 436 128 199 4.42e-16 79.3
MS.gene66598.t1 MTR_5g043880 47.436 78 40 1 346 423 104 180 4.51e-16 79.3
MS.gene66598.t1 MTR_1g099600 40.860 93 53 1 364 456 183 273 2.08e-15 78.2
MS.gene66598.t1 MTR_1g077780 46.053 76 40 1 353 428 49 123 3.25e-15 73.6
MS.gene66598.t1 MTR_8g092140 39.000 100 59 2 325 423 118 216 3.75e-15 75.9
MS.gene66598.t1 MTR_3g095040 45.333 75 40 1 353 427 105 178 1.85e-14 73.2
MS.gene66598.t1 MTR_1g078260 47.222 72 36 2 353 423 234 304 1.90e-13 73.6
MS.gene66598.t1 MTR_6g086555 46.250 80 41 2 353 431 193 271 2.69e-13 72.8
MS.gene66598.t1 MTR_3g108960 42.029 69 39 1 355 423 304 371 9.28e-13 70.9
MS.gene66598.t1 MTR_3g031220 40.230 87 46 3 344 430 180 260 1.93e-12 69.7
MS.gene66598.t1 MTR_4g007060 40.984 61 36 0 343 403 139 199 2.97e-12 67.4
MS.gene66598.t1 MTR_0117s0040 42.029 69 39 1 355 423 64 131 3.83e-12 67.0
MS.gene66598.t1 MTR_3g009470 43.750 64 34 2 367 430 281 342 2.28e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66598.t1 AT4G04450 39.925 531 221 16 65 577 53 503 1.07e-84 276
MS.gene66598.t1 AT4G04450 41.079 482 193 15 114 577 1 409 1.53e-80 262
MS.gene66598.t1 AT1G62300 50.187 267 110 8 345 593 291 552 4.20e-66 227
MS.gene66598.t1 AT4G22070 54.000 250 87 6 345 577 276 514 1.18e-60 212
MS.gene66598.t1 AT4G22070 54.000 250 87 6 345 577 276 514 1.18e-60 212
MS.gene66598.t1 AT4G22070 54.000 250 87 6 345 577 276 514 1.18e-60 212
MS.gene66598.t1 AT4G22070 54.000 250 87 6 345 577 276 514 1.18e-60 212
MS.gene66598.t1 AT4G01720 42.712 295 128 8 300 574 170 443 2.02e-46 171
MS.gene66598.t1 AT1G68150 61.856 97 37 0 351 447 220 316 2.23e-38 146
MS.gene66598.t1 AT5G15130 35.793 271 123 9 346 578 165 422 6.18e-38 148
MS.gene66598.t1 AT5G15130 35.793 271 123 9 346 578 207 464 7.59e-38 148
MS.gene66598.t1 AT1G69810 45.455 176 85 5 320 494 103 268 4.85e-36 138
MS.gene66598.t1 AT1G69810 45.455 176 85 5 320 494 161 326 6.15e-36 139
MS.gene66598.t1 AT1G18860 30.311 386 202 12 139 504 48 386 3.27e-33 134
MS.gene66598.t1 AT2G03340 44.828 116 54 2 313 428 371 476 2.11e-24 107
MS.gene66598.t1 AT2G03340 51.667 60 27 2 367 426 251 308 1.25e-11 67.8
MS.gene66598.t1 AT1G13960 55.696 79 34 1 348 426 238 315 3.99e-24 105
MS.gene66598.t1 AT1G13960 47.222 72 36 2 367 438 77 146 7.01e-12 67.8
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 188 266 4.59e-24 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 188 266 4.59e-24 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 188 266 4.59e-24 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 188 266 4.59e-24 105
MS.gene66598.t1 AT1G13960 55.696 79 34 1 348 426 391 468 5.69e-24 106
MS.gene66598.t1 AT1G13960 41.111 90 46 3 354 438 212 299 5.81e-12 68.9
MS.gene66598.t1 AT2G38470 54.321 81 36 1 348 428 344 423 1.09e-23 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 292 370 1.35e-23 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 324 402 1.44e-23 105
MS.gene66598.t1 AT4G26640 52.500 80 37 1 349 428 364 442 1.84e-23 105
MS.gene66598.t1 AT4G31800 43.810 105 54 2 345 444 85 189 2.18e-22 97.4
MS.gene66598.t1 AT4G31800 43.810 105 54 2 345 444 150 254 2.74e-22 98.6
MS.gene66598.t1 AT5G56270 53.086 81 37 1 348 428 469 548 5.94e-22 101
MS.gene66598.t1 AT2G25000 32.584 178 103 4 266 429 14 188 8.28e-22 95.5
MS.gene66598.t1 AT3G01080 48.837 86 43 1 341 426 319 403 9.07e-22 99.4
MS.gene66598.t1 AT2G04880 45.055 91 49 1 336 426 253 342 9.56e-22 99.4
MS.gene66598.t1 AT2G04880 49.275 69 31 3 362 429 107 172 9.74e-12 68.2
MS.gene66598.t1 AT3G01080 48.837 86 43 1 341 426 281 365 1.08e-21 98.6
MS.gene66598.t1 AT2G04880 45.055 91 49 1 336 426 277 366 1.26e-21 99.0
MS.gene66598.t1 AT2G04880 49.275 69 31 3 362 429 107 172 1.05e-11 68.2
MS.gene66598.t1 AT1G80840 47.436 78 39 1 352 427 130 207 3.46e-21 95.1
MS.gene66598.t1 AT1G29860 46.809 94 45 2 351 444 121 209 3.54e-21 94.7
MS.gene66598.t1 AT5G07100 48.235 85 43 1 346 430 121 204 3.93e-21 92.8
MS.gene66598.t1 AT5G07100 48.235 85 43 1 346 430 121 204 3.93e-21 92.8
MS.gene66598.t1 AT5G07100 48.235 85 43 1 346 430 121 204 3.93e-21 92.8
MS.gene66598.t1 AT5G07100 48.235 85 43 1 346 430 121 204 3.93e-21 92.8
MS.gene66598.t1 AT2G25000 34.211 152 94 3 281 429 18 166 5.23e-21 92.8
MS.gene66598.t1 AT2G25000 34.211 152 94 3 281 429 18 166 5.23e-21 92.8
MS.gene66598.t1 AT5G07100 48.235 85 43 1 346 430 214 297 7.29e-21 94.4
MS.gene66598.t1 AT2G25000 34.211 152 94 3 281 429 61 209 1.02e-20 93.2
MS.gene66598.t1 AT4G18170 44.000 100 55 1 353 452 159 257 1.22e-20 94.0
MS.gene66598.t1 AT5G41570 45.455 99 49 2 353 451 85 178 1.64e-20 89.7
MS.gene66598.t1 AT1G64000 53.247 77 35 1 348 424 96 171 1.73e-20 90.5
MS.gene66598.t1 AT5G26170 54.167 72 32 1 352 423 99 169 1.78e-20 89.7
MS.gene66598.t1 AT5G46350 54.054 74 33 1 353 426 170 242 8.70e-20 91.3
MS.gene66598.t1 AT2G30250 46.512 86 45 1 345 430 307 391 1.41e-19 92.0
MS.gene66598.t1 AT2G23320 59.322 59 24 0 367 425 241 299 1.69e-19 90.5
MS.gene66598.t1 AT5G49520 46.250 80 42 1 351 430 206 284 2.83e-19 90.9
MS.gene66598.t1 AT1G29280 50.000 70 35 0 357 426 65 134 3.58e-19 88.2
MS.gene66598.t1 AT4G30935 40.000 125 71 3 334 456 299 421 5.41e-19 90.9
MS.gene66598.t1 AT2G30590 57.812 64 26 1 367 429 314 377 5.84e-19 89.7
MS.gene66598.t1 AT5G13080 52.055 73 34 1 353 425 54 125 1.14e-18 83.6
MS.gene66598.t1 AT1G55600 48.684 76 38 1 353 428 294 368 1.30e-18 89.7
MS.gene66598.t1 AT4G26440 51.316 76 36 1 353 428 359 433 2.01e-18 89.7
MS.gene66598.t1 AT3G58710 47.945 73 38 0 357 429 62 134 2.20e-18 86.3
MS.gene66598.t1 AT3G58710 47.945 73 38 0 357 429 61 133 2.42e-18 85.9
MS.gene66598.t1 AT5G28650 60.377 53 21 0 371 423 267 319 5.35e-18 86.3
MS.gene66598.t1 AT4G24240 56.667 60 26 0 366 425 281 340 8.31e-18 85.9
MS.gene66598.t1 AT3G01970 51.389 72 34 1 352 423 51 121 1.03e-17 80.9
MS.gene66598.t1 AT3G62340 52.703 74 34 1 353 426 105 177 1.06e-17 84.3
MS.gene66598.t1 AT3G04670 60.377 53 21 0 371 423 267 319 1.40e-17 84.7
MS.gene66598.t1 AT2G24570 59.649 57 23 0 367 423 244 300 1.59e-17 84.7
MS.gene66598.t1 AT2G47260 45.783 83 44 1 351 433 159 240 1.60e-17 84.7
MS.gene66598.t1 AT2G44745 39.286 112 63 3 315 423 92 201 1.64e-17 82.4
MS.gene66598.t1 AT2G46130 46.429 84 43 2 342 424 5 87 1.84e-17 79.0
MS.gene66598.t1 AT4G01250 46.429 84 43 1 353 436 117 198 2.80e-17 83.6
MS.gene66598.t1 AT2G34830 43.011 93 46 1 348 433 190 282 3.02e-17 85.1
MS.gene66598.t1 AT5G52830 45.455 77 42 0 360 436 159 235 3.03e-17 84.3
MS.gene66598.t1 AT1G30650 43.011 93 46 1 348 433 192 284 3.22e-17 85.1
MS.gene66598.t1 AT2G34830 43.011 93 46 1 348 433 199 291 3.69e-17 84.7
MS.gene66598.t1 AT4G31550 57.895 57 24 0 367 423 247 303 4.03e-17 83.6
MS.gene66598.t1 AT4G31550 57.895 57 24 0 367 423 246 302 4.12e-17 83.6
MS.gene66598.t1 AT5G64810 45.745 94 47 2 333 423 74 166 6.04e-17 80.1
MS.gene66598.t1 AT5G45050 26.939 245 156 7 294 529 971 1201 1.09e-16 85.1
MS.gene66598.t1 AT5G45050 28.311 219 138 6 316 529 1108 1312 1.12e-16 85.1
MS.gene66598.t1 AT5G45050 28.311 219 138 6 316 529 1136 1340 1.13e-16 85.1
MS.gene66598.t1 AT4G23550 44.872 78 43 0 361 438 135 212 2.22e-16 80.9
MS.gene66598.t1 AT4G39410 52.113 71 33 1 353 423 210 279 5.38e-16 79.7
MS.gene66598.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.26e-15 78.2
MS.gene66598.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.26e-15 78.2
MS.gene66598.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.26e-15 78.2
MS.gene66598.t1 AT2G37260 53.846 65 29 1 361 425 264 327 2.51e-15 78.6
MS.gene66598.t1 AT2G37260 48.529 68 28 3 367 429 86 151 2.03e-11 66.6
MS.gene66598.t1 AT2G37260 53.846 65 29 1 361 425 262 325 2.55e-15 78.2
MS.gene66598.t1 AT2G37260 48.529 68 28 3 367 429 84 149 2.09e-11 66.2
MS.gene66598.t1 AT2G37260 53.846 65 29 1 361 425 344 407 3.71e-15 78.6
MS.gene66598.t1 AT2G37260 48.529 68 28 3 367 429 166 231 2.38e-11 66.6
MS.gene66598.t1 AT2G21900 42.029 69 39 1 361 429 104 171 2.67e-13 69.7
MS.gene66598.t1 AT2G46130 44.048 84 41 3 342 424 5 83 4.75e-13 66.2
MS.gene66598.t1 AT5G45260 31.847 157 87 4 296 440 1136 1284 8.43e-13 72.4
MS.gene66598.t1 AT4G31800 48.438 64 28 1 345 403 150 213 5.53e-12 66.6
MS.gene66598.t1 AT5G43290 33.824 136 81 7 292 423 40 170 5.67e-11 64.3

Find 113 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACTGATCTTGACTTCT+AGG 0.104418 4.3:-49883416 None:intergenic
TACTGATACACCATCTTTAT+TGG 0.104474 4.3:+49881398 MS.gene66598:CDS
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4.3:-49884750 None:intergenic
GACAAAGCTGCAAGAATATT+TGG 0.206223 4.3:-49884927 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4.3:-49881669 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4.3:+49884951 MS.gene66598:CDS
TAAAGTTTAGGTCTTGTAAT+TGG 0.259816 4.3:-49885142 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4.3:+49884750 MS.gene66598:CDS
ACTGGGGCCAAGGAAATTTA+TGG 0.276997 4.3:+49883085 MS.gene66598:CDS
ACTGTTTGAGCAGTAATATT+TGG 0.280261 4.3:-49884882 None:intergenic
GGGAAAGAGTTGTTATTAAC+TGG 0.286280 4.3:-49881153 None:intergenic
GCGTTCCTTTGTGGAGTATT+TGG 0.301041 4.3:-49884711 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4.3:+49883005 MS.gene66598:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4.3:+49883245 MS.gene66598:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4.3:+49882896 MS.gene66598:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.335137 4.3:+49883125 MS.gene66598:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4.3:+49884854 MS.gene66598:CDS
CCAAGGAAATTTATGGAACT+TGG 0.341755 4.3:+49883092 MS.gene66598:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4.3:-49883779 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4.3:+49883684 MS.gene66598:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4.3:-49881615 None:intergenic
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4.3:-49884516 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4.3:+49881680 MS.gene66598:CDS
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4.3:+49883067 MS.gene66598:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4.3:-49883735 None:intergenic
TCCCTTTCCTAGTCAATATA+AGG 0.384368 4.3:+49881244 MS.gene66598:CDS
AACTGATTTGCGTTCCTTTG+TGG 0.385254 4.3:-49884720 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4.3:+49882873 MS.gene66598:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4.3:+49883212 MS.gene66598:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4.3:+49885002 MS.gene66598:CDS
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4.3:-49884510 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4.3:-49884675 None:intergenic
GAAATTTATGGAACTTGGAT+TGG 0.404712 4.3:+49883097 MS.gene66598:CDS
ACTTGGATTGGCTACTGATA+AGG 0.406023 4.3:+49883109 MS.gene66598:CDS
TAACATCCTCATTCTTACTT+AGG 0.406214 4.3:-49883481 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.408470 4.3:-49884984 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4.3:-49883004 None:intergenic
GCTACCATTGCTATTTCCTT+CGG 0.410299 4.3:-49885036 None:intergenic
CTTGTTTCATGAGATGAGAT+TGG 0.411363 4.3:-49881291 None:intergenic
CTGTAATCAATTGTAATAGT+TGG 0.412579 4.3:-49884681 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4.3:-49881174 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4.3:-49884509 None:intergenic
CCAAGTTCCATAAATTTCCT+TGG 0.423079 4.3:-49883092 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4.3:-49883773 None:intergenic
TGATGGGAAGGAACAAAATC+AGG 0.431895 4.3:+49885115 MS.gene66598:CDS
AAAATGAGAATTAAGAATGA+TGG 0.434196 4.3:+49883179 MS.gene66598:CDS
TGGCTGAGTATCACCAATAA+TGG 0.440113 4.3:-49884964 None:intergenic
GTATGAATATGATCATTGTC+AGG 0.443290 4.3:-49881378 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.444001 4.3:+49883115 MS.gene66598:CDS
AACTTTAGTTCCAATAAAGA+TGG 0.454904 4.3:-49881408 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4.3:+49883235 MS.gene66598:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4.3:+49884515 MS.gene66598:CDS
GACCTTATATTGACTAGGAA+AGG 0.470626 4.3:-49881246 None:intergenic
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4.3:+49883068 MS.gene66598:CDS
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4.3:+49883416 MS.gene66598:CDS
ACATGTGGCATCAACAATGA+TGG 0.482281 4.3:+49885098 MS.gene66598:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4.3:+49883217 MS.gene66598:CDS
GCATCAACATCAAGCAATGA+TGG 0.494138 4.3:+49884576 MS.gene66598:CDS
GTTGGTGTCAAGGCAATGTA+AGG 0.496162 4.3:+49883325 MS.gene66598:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4.3:+49883229 MS.gene66598:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4.3:-49883764 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4.3:+49883195 MS.gene66598:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4.3:-49883774 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4.3:+49882989 MS.gene66598:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4.3:-49884861 None:intergenic
TCATGAAACAAGAACAGAGT+TGG 0.518840 4.3:+49881302 MS.gene66598:CDS
TAGTAGTGATCAATCTATGA+TGG 0.520634 4.3:+49881632 MS.gene66598:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4.3:-49883729 None:intergenic
ACCTTATATTGACTAGGAAA+GGG 0.537487 4.3:-49881245 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4.3:+49883663 MS.gene66598:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4.3:-49881191 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.543545 4.3:+49884474 MS.gene66598:CDS
ATGGTGGAGTACTGGGGCCA+AGG 0.545172 4.3:+49883075 MS.gene66598:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4.3:-49883763 None:intergenic
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4.3:-49884758 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4.3:+49883307 MS.gene66598:CDS
AGTATTGATAGTAATAGGAA+TGG 0.556477 4.3:-49881610 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4.3:+49883263 MS.gene66598:CDS
GTATTGATAGTAATAGGAAT+GGG 0.563883 4.3:-49881609 None:intergenic
TATCGCTGCAGCACGACCCA+TGG 0.564450 4.3:+49883747 MS.gene66598:CDS
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4.3:+49881683 MS.gene66598:CDS
GATAATAAGCTTTAGGACAC+GGG 0.575823 4.3:-49883728 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4.3:+49881639 MS.gene66598:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4.3:+49883673 MS.gene66598:intron
AGCAACAAAATTATCACATG+TGG 0.580366 4.3:+49885083 MS.gene66598:CDS
GTATTGCCAATATTTGTGAA+GGG 0.582929 4.3:-49884860 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4.3:+49885099 MS.gene66598:CDS
TACAAATGCTGATATTGTGA+TGG 0.589604 4.3:+49883361 MS.gene66598:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4.3:+49883475 MS.gene66598:CDS
ATGATCATTGTCAGGGACAG+AGG 0.594495 4.3:-49881370 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.594834 4.3:-49881377 None:intergenic
ATGGTGGAGAGTTGGTGTCA+AGG 0.595504 4.3:+49883315 MS.gene66598:CDS
TGGCATCAACAATGATGGGA+AGG 0.604261 4.3:+49885103 MS.gene66598:CDS
ATTAACTGGAACTGAAGCCA+TGG 0.605747 4.3:-49881139 None:intergenic
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4.3:-49884778 None:intergenic
AATATTTGGACTAGCAGCAA+TGG 0.610076 4.3:-49884913 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4.3:+49883685 MS.gene66598:CDS
TGGAGGACCTTATATTGACT+AGG 0.616940 4.3:-49881251 None:intergenic
TTAAAACTTAACAGGTACAA+AGG 0.618245 4.3:+49884433 MS.gene66598:intron
TGTTAATGTTGATAATGTTG+AGG 0.619880 4.3:+49883499 MS.gene66598:CDS
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4.3:-49884506 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4.3:-49881173 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.626521 4.3:+49885032 MS.gene66598:CDS
AATGGCAACAATGTCACCGA+AGG 0.626879 4.3:+49885020 MS.gene66598:CDS
AGCTAGATCAGAAGAAACCA+TGG 0.628836 4.3:+49883553 MS.gene66598:CDS
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4.3:-49884759 None:intergenic
TTCATGAGATGAGATTGGAG+AGG 0.650096 4.3:-49881286 None:intergenic
AGGCAGAGACAACTTCATGA+TGG 0.653739 4.3:-49881350 None:intergenic
GAATAGATTACAATTACATG+CGG 0.660089 4.3:+49882955 MS.gene66598:CDS
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4.3:+49883069 MS.gene66598:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4.3:-49884636 None:intergenic
AATCTCCAAATACTCCACAA+AGG 0.705445 4.3:+49884706 MS.gene66598:CDS
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4.3:+49882990 MS.gene66598:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATCACATAAAAAA+CGG - chr4.3:49882837-49882856 None:intergenic 10.0%
!! ATATATATATATATGATTCT+TGG + chr4.3:49882009-49882028 MS.gene66598:intron 10.0%
!! GTAAAAAAAATAATAACAAT+GGG - chr4.3:49881460-49881479 None:intergenic 10.0%
!! TATAACATAATTATTATTTG+TGG - chr4.3:49882152-49882171 None:intergenic 10.0%
!!! ATTAATTCTTTTTAGTAAAA+AGG - chr4.3:49883641-49883660 None:intergenic 10.0%
!!! TTATTTTCTCAAATTATTAT+TGG + chr4.3:49882542-49882561 MS.gene66598:intron 10.0%
!! AGCAAAAAAAATTAAAGTTT+AGG - chr4.3:49885157-49885176 None:intergenic 15.0%
!! CGTAAAAAAAATAATAACAA+TGG - chr4.3:49881461-49881480 None:intergenic 15.0%
!! TAATACAATTAAAACTTAAC+AGG + chr4.3:49884425-49884444 MS.gene66598:intron 15.0%
!! TAATTAAATTACTTATGAAG+TGG + chr4.3:49884381-49884400 MS.gene66598:intron 15.0%
!! TGTATTATACTTATAAATGT+AGG - chr4.3:49882076-49882095 None:intergenic 15.0%
!! TTTAGTAAATACATGTAATT+TGG - chr4.3:49883813-49883832 None:intergenic 15.0%
!!! TTCTACTATGTTATTTTTTT+TGG + chr4.3:49881929-49881948 MS.gene66598:intron 15.0%
!!! TTTTGACTCAATAAAATTAA+AGG - chr4.3:49881773-49881792 None:intergenic 15.0%
!! AAAAAATTTAAAAGAAAGCG+AGG - chr4.3:49881911-49881930 None:intergenic 20.0%
!! AAAATGAGAATTAAGAATGA+TGG + chr4.3:49883179-49883198 MS.gene66598:CDS 20.0%
!! ATATATATATGATTCTTGGT+TGG + chr4.3:49882013-49882032 MS.gene66598:intron 20.0%
!! TAATTTCATTAAATTCTGCT+AGG - chr4.3:49881729-49881748 None:intergenic 20.0%
!!! AATTTTATTGAGTCAAAACT+GGG + chr4.3:49881775-49881794 MS.gene66598:intron 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG - chr4.3:49884807-49884826 None:intergenic 20.0%
!!! ACATGCATATTTTAAAACAT+TGG + chr4.3:49883988-49884007 MS.gene66598:intron 20.0%
!!! TAATTTTATTGAGTCAAAAC+TGG + chr4.3:49881774-49881793 MS.gene66598:intron 20.0%
! AAAATGTAACAGTTTATGTC+AGG - chr4.3:49882516-49882535 None:intergenic 25.0%
! AAAGGAAAAAAAGTCTTTGA+TGG + chr4.3:49883023-49883042 MS.gene66598:CDS 25.0%
! AACAATGGGATATTGAATTA+TGG - chr4.3:49881446-49881465 None:intergenic 25.0%
! ATTTAATTAGCGTACTATGT+CGG - chr4.3:49882340-49882359 None:intergenic 25.0%
! CAATAAACTCAAATCCAAAA+TGG + chr4.3:49884341-49884360 MS.gene66598:intron 25.0%
! CACTGACATACATATAAAAT+CGG - chr4.3:49882597-49882616 None:intergenic 25.0%
! CCAACAAAATCAAAATACAT+TGG - chr4.3:49882737-49882756 None:intergenic 25.0%
! CTGTAATCAATTGTAATAGT+TGG - chr4.3:49884684-49884703 None:intergenic 25.0%
! GAATAGATTACAATTACATG+CGG + chr4.3:49882955-49882974 MS.gene66598:CDS 25.0%
! GCTGCTGATAATAATAATAA+TGG + chr4.3:49885002-49885021 MS.gene66598:CDS 25.0%
! GTCATATTCATTAATTAACG+TGG - chr4.3:49882244-49882263 None:intergenic 25.0%
! TAAATACATAGATTGCTGAA+TGG - chr4.3:49884201-49884220 None:intergenic 25.0%
! TAGAACACTATTAACATGAT+GGG + chr4.3:49882709-49882728 MS.gene66598:intron 25.0%
! TATGAATATGATCATTGTCA+GGG - chr4.3:49881380-49881399 None:intergenic 25.0%
! TCAAAATATGCAGTTATGTT+TGG + chr4.3:49884251-49884270 MS.gene66598:intron 25.0%
! TCAATTGTAATAGTTGGATA+TGG - chr4.3:49884678-49884697 None:intergenic 25.0%
! TTAAAACTTAACAGGTACAA+AGG + chr4.3:49884433-49884452 MS.gene66598:intron 25.0%
! TTAGAACACTATTAACATGA+TGG + chr4.3:49882708-49882727 MS.gene66598:intron 25.0%
!! AACTTTAGTTCCAATAAAGA+TGG - chr4.3:49881411-49881430 None:intergenic 25.0%
!! AATCACATAAAAAACGGATT+TGG - chr4.3:49882831-49882850 None:intergenic 25.0%
!! ACTAAAAATGTTTCTGTATG+TGG - chr4.3:49884063-49884082 None:intergenic 25.0%
!! ATGATGGAGAATTAATGAAA+AGG + chr4.3:49883195-49883214 MS.gene66598:CDS 25.0%
!! CAAAACCAATCAAAATTTTC+TGG + chr4.3:49884810-49884829 MS.gene66598:CDS 25.0%
!! CATGTTAATAGTGTTCTAAA+AGG - chr4.3:49882707-49882726 None:intergenic 25.0%
!! GTATTGATAGTAATAGGAAT+GGG - chr4.3:49881612-49881631 None:intergenic 25.0%
!! TAAAGTTTAGGTCTTGTAAT+TGG - chr4.3:49885145-49885164 None:intergenic 25.0%
!! TATGTTTCTATGCTAGAAAA+TGG + chr4.3:49884162-49884181 MS.gene66598:intron 25.0%
!! TGTGATAATTTTGTTGCTAT+TGG - chr4.3:49885081-49885100 None:intergenic 25.0%
!! TTCATTGAACTATTTTCAGA+TGG + chr4.3:49882860-49882879 MS.gene66598:intron 25.0%
!!! AGTATTGATAGTAATAGGAA+TGG - chr4.3:49881613-49881632 None:intergenic 25.0%
!!! CCAATGTATTTTGATTTTGT+TGG + chr4.3:49882734-49882753 MS.gene66598:intron 25.0%
!!! CTACTAGTATTGATAGTAAT+AGG - chr4.3:49881618-49881637 None:intergenic 25.0%
!!! CTGCATATTTTGATTTACAA+TGG - chr4.3:49884244-49884263 None:intergenic 25.0%
!!! GTAATTTTTTCACAAAGAAG+AGG - chr4.3:49882647-49882666 None:intergenic 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG + chr4.3:49883499-49883518 MS.gene66598:CDS 25.0%
AAGAAAAAGAGACTTCAGAA+TGG + chr4.3:49883296-49883315 MS.gene66598:CDS 30.0%
ACGTTCGAATAAAACATGTT+AGG - chr4.3:49882800-49882819 None:intergenic 30.0%
ACTGTTTGAGCAGTAATATT+TGG - chr4.3:49884885-49884904 None:intergenic 30.0%
AGAACACTATTAACATGATG+GGG + chr4.3:49882710-49882729 MS.gene66598:intron 30.0%
AGAAGAAACTTACATGCTTT+CGG - chr4.3:49883782-49883801 None:intergenic 30.0%
AGCAACAAAATTATCACATG+TGG + chr4.3:49885083-49885102 MS.gene66598:CDS 30.0%
AGTTTATGTCAGGATCATAT+TGG - chr4.3:49882506-49882525 None:intergenic 30.0%
ATAATGCGAAAGCAATCAAT+AGG - chr4.3:49883854-49883873 None:intergenic 30.0%
CGTTCGAATAAAACATGTTA+GGG - chr4.3:49882799-49882818 None:intergenic 30.0%
GAAATTTATGGAACTTGGAT+TGG + chr4.3:49883097-49883116 MS.gene66598:CDS 30.0%
GATAAGGTTAAGGAAGTTAA+AGG + chr4.3:49883125-49883144 MS.gene66598:CDS 30.0%
GTATGAATATGATCATTGTC+AGG - chr4.3:49881381-49881400 None:intergenic 30.0%
GTATTGCCAATATTTGTGAA+GGG - chr4.3:49884863-49884882 None:intergenic 30.0%
GTTGTAATCACAACTTATGA+AGG + chr4.3:49884474-49884493 MS.gene66598:CDS 30.0%
TAACATCCTCATTCTTACTT+AGG - chr4.3:49883484-49883503 None:intergenic 30.0%
TAATGCGAAAGCAATCAATA+GGG - chr4.3:49883853-49883872 None:intergenic 30.0%
TACAAATGCTGATATTGTGA+TGG + chr4.3:49883361-49883380 MS.gene66598:CDS 30.0%
TACTGATACACCATCTTTAT+TGG + chr4.3:49881398-49881417 MS.gene66598:CDS 30.0%
TAGTAGTGATCAATCTATGA+TGG + chr4.3:49881632-49881651 MS.gene66598:CDS 30.0%
TTATTGAGTTAGATCACTGA+TGG + chr4.3:49883663-49883682 MS.gene66598:intron 30.0%
! ACACTTTTACATACTTACCA+TGG - chr4.3:49883573-49883592 None:intergenic 30.0%
! ACCTTATATTGACTAGGAAA+GGG - chr4.3:49881248-49881267 None:intergenic 30.0%
! CCTCAATACTCAAATTTTCA+GGG + chr4.3:49882468-49882487 MS.gene66598:intron 30.0%
! CTTTAACTTTCTTACCCTTA+CGG - chr4.3:49883007-49883026 None:intergenic 30.0%
! TATATGATTCTTGGTTGGTT+TGG + chr4.3:49882018-49882037 MS.gene66598:intron 30.0%
!! AAAAGGAAATTTGATGATGC+TGG + chr4.3:49883212-49883231 MS.gene66598:CDS 30.0%
!! AGAAACATAAGCTTTGAAAG+TGG - chr4.3:49884151-49884170 None:intergenic 30.0%
!! CCTGAAAATTTGAGTATTGA+GGG - chr4.3:49882470-49882489 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG - chr4.3:49884818-49884837 None:intergenic 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG - chr4.3:49884529-49884548 None:intergenic 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG - chr4.3:49884797-49884816 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG - chr4.3:49884798-49884817 None:intergenic 30.0%
!!! TTATTTTTTCGCATACAGCT+TGG + chr4.3:49881585-49881604 MS.gene66598:intron 30.0%
AAAAAGAGAACTGAGAATGG+TGG + chr4.3:49883059-49883078 MS.gene66598:CDS 35.0%
AAAAAGAGACTTCAGAATGG+TGG + chr4.3:49883299-49883318 MS.gene66598:CDS 35.0%
AAAATGGTAGCTACTTAGCT+AGG + chr4.3:49884357-49884376 MS.gene66598:intron 35.0%
AATCTCCAAATACTCCACAA+AGG + chr4.3:49884706-49884725 MS.gene66598:CDS 35.0%
ACTTAGTCCCAACAAAAACA+TGG + chr4.3:49881203-49881222 MS.gene66598:CDS 35.0%
AGAGAAGAGAACTAAGAGTA+TGG + chr4.3:49881680-49881699 MS.gene66598:CDS 35.0%
AGAGCTTGAACAAACGAAAA+TGG + chr4.3:49882896-49882915 MS.gene66598:CDS 35.0%
AGGAATTTCCAAAACTCTGA+GGG - chr4.3:49884761-49884780 None:intergenic 35.0%
ATGGTCGTTGTATCAAAGAT+TGG - chr4.3:49884282-49884301 None:intergenic 35.0%
ATTGGCTACTGATAAGGTTA+AGG + chr4.3:49883115-49883134 MS.gene66598:CDS 35.0%
CAAGGTTGTTAGTATCATCA+GGG - chr4.3:49881176-49881195 None:intergenic 35.0%
CATGTTTGTGACTATGATCT+AGG - chr4.3:49883973-49883992 None:intergenic 35.0%
CCAAGGAAATTTATGGAACT+TGG + chr4.3:49883092-49883111 MS.gene66598:CDS 35.0%
CCAAGTTCCATAAATTTCCT+TGG - chr4.3:49883095-49883114 None:intergenic 35.0%
CGTAAGGGTAAGAAAGTTAA+AGG + chr4.3:49883005-49883024 MS.gene66598:CDS 35.0%
CTTGTTTCATGAGATGAGAT+TGG - chr4.3:49881294-49881313 None:intergenic 35.0%
GACAAAGCTGCAAGAATATT+TGG - chr4.3:49884930-49884949 None:intergenic 35.0%
GAGAAAAAGAGAACTGAGAA+TGG + chr4.3:49883056-49883075 MS.gene66598:CDS 35.0%
GATAGTCCTAAGTAAGAATG+AGG + chr4.3:49883475-49883494 MS.gene66598:CDS 35.0%
GATCTCACTATTACAATGCA+TGG + chr4.3:49882681-49882700 MS.gene66598:intron 35.0%
GCTGCTATTACATCCATTAT+TGG + chr4.3:49884951-49884970 MS.gene66598:CDS 35.0%
GGTATTGCCAATATTTGTGA+AGG - chr4.3:49884864-49884883 None:intergenic 35.0%
GTTCTCTTCTCTTCTGAATT+AGG - chr4.3:49881672-49881691 None:intergenic 35.0%
GTTGCAATACTTGAAGAACA+AGG - chr4.3:49884639-49884658 None:intergenic 35.0%
TATGGGCAAAAACTATCGAA+AGG + chr4.3:49883702-49883721 MS.gene66598:CDS 35.0%
TCAAGGTTGTTAGTATCATC+AGG - chr4.3:49881177-49881196 None:intergenic 35.0%
TCACGTAATAAGCACATTGA+TGG - chr4.3:49884301-49884320 None:intergenic 35.0%
TCATGAAACAAGAACAGAGT+TGG + chr4.3:49881302-49881321 MS.gene66598:CDS 35.0%
TCCCTTTCCTAGTCAATATA+AGG + chr4.3:49881244-49881263 MS.gene66598:CDS 35.0%
TCGACACTCTTTAGATTTGA+AGG + chr4.3:49881815-49881834 MS.gene66598:intron 35.0%
TTATTGCATTCAGCAAATCC+AGG - chr4.3:49884327-49884346 None:intergenic 35.0%
TTTCAGATGGCTATTCTTCA+AGG + chr4.3:49882873-49882892 MS.gene66598:intron 35.0%
! CCCTCAATACTCAAATTTTC+AGG + chr4.3:49882467-49882486 MS.gene66598:intron 35.0%
! CCCTGAAAATTTGAGTATTG+AGG - chr4.3:49882471-49882490 None:intergenic 35.0%
! GACCTTATATTGACTAGGAA+AGG - chr4.3:49881249-49881268 None:intergenic 35.0%
! GATGTCAATGGAGAAAGTAT+GGG + chr4.3:49883685-49883704 MS.gene66598:CDS 35.0%
! GGGAAAGAGTTGTTATTAAC+TGG - chr4.3:49881156-49881175 None:intergenic 35.0%
! TAGCTAAGTAGCTACCATTT+TGG - chr4.3:49884358-49884377 None:intergenic 35.0%
!! AAAGGAAAAGAAGTGTTCAC+TGG + chr4.3:49883263-49883282 MS.gene66598:CDS 35.0%
!! AATATTTGGACTAGCAGCAA+TGG - chr4.3:49884916-49884935 None:intergenic 35.0%
!! AGAGGTTTGATTTTCATTGG+AGG - chr4.3:49881271-49881290 None:intergenic 35.0%
!! ATGTTTTATTCGAACGTGAG+AGG + chr4.3:49882803-49882822 MS.gene66598:intron 35.0%
!! CGATAATAAGCTTTAGGACA+CGG - chr4.3:49883732-49883751 None:intergenic 35.0%
!! GAAATTTGATGATGCTGGAT+TGG + chr4.3:49883217-49883236 MS.gene66598:CDS 35.0%
!! GATAATAAGCTTTAGGACAC+GGG - chr4.3:49883731-49883750 None:intergenic 35.0%
!! GATCAATCTATGATGGATGA+TGG + chr4.3:49881639-49881658 MS.gene66598:CDS 35.0%
!! GTAGCATCCATGTTTTTGTT+GGG - chr4.3:49881213-49881232 None:intergenic 35.0%
!! TCTTTAGATTTGAAGGTGTC+TGG + chr4.3:49881822-49881841 MS.gene66598:intron 35.0%
!! TGGAGAGGTTTGATTTTCAT+TGG - chr4.3:49881274-49881293 None:intergenic 35.0%
!! TGTAGCATCCATGTTTTTGT+TGG - chr4.3:49881214-49881233 None:intergenic 35.0%
AAACTTACATGCTTTCGGAC+TGG - chr4.3:49883777-49883796 None:intergenic 40.0%
AACTTACATGCTTTCGGACT+GGG - chr4.3:49883776-49883795 None:intergenic 40.0%
ACATGTGGCATCAACAATGA+TGG + chr4.3:49885098-49885117 MS.gene66598:CDS 40.0%
AGCTAGATCAGAAGAAACCA+TGG + chr4.3:49883553-49883572 MS.gene66598:CDS 40.0%
AGCTGACCCTTCACAAATAT+TGG + chr4.3:49884854-49884873 MS.gene66598:CDS 40.0%
CATGTGGCATCAACAATGAT+GGG + chr4.3:49885099-49885118 MS.gene66598:CDS 40.0%
CCTAACTGATCTTGACTTCT+AGG - chr4.3:49883419-49883438 None:intergenic 40.0%
CCTAGAAGTCAAGATCAGTT+AGG + chr4.3:49883416-49883435 MS.gene66598:CDS 40.0%
CTGCAGCGATAATAAGCTTT+AGG - chr4.3:49883738-49883757 None:intergenic 40.0%
GAGGAATTTCCAAAACTCTG+AGG - chr4.3:49884762-49884781 None:intergenic 40.0%
GATTTGATATGCCTTGCACA+TGG + chr4.3:49884091-49884110 MS.gene66598:intron 40.0%
GCATCAACATCAAGCAATGA+TGG + chr4.3:49884576-49884595 MS.gene66598:CDS 40.0%
GCCCACGTACACAAAAATAT+GGG + chr4.3:49881878-49881897 MS.gene66598:intron 40.0%
GGATCATATTGGTGCAATGA+CGG - chr4.3:49882495-49882514 None:intergenic 40.0%
GGATGTCAATGGAGAAAGTA+TGG + chr4.3:49883684-49883703 MS.gene66598:CDS 40.0%
GTACACAAAAATATGGGCCA+AGG + chr4.3:49881884-49881903 MS.gene66598:intron 40.0%
TGATGGGAAGGAACAAAATC+AGG + chr4.3:49885115-49885134 MS.gene66598:CDS 40.0%
TGGCTGAGTATCACCAATAA+TGG - chr4.3:49884967-49884986 None:intergenic 40.0%
TGGGACTAAGTTTCAACTCA+AGG - chr4.3:49881194-49881213 None:intergenic 40.0%
TTAAAAGAAAGCGAGGACCT+TGG - chr4.3:49881904-49881923 None:intergenic 40.0%
TTAACGTGGCAGCATATCAT+TGG - chr4.3:49882230-49882249 None:intergenic 40.0%
TTCATGAGATGAGATTGGAG+AGG - chr4.3:49881289-49881308 None:intergenic 40.0%
TTGTTATCCAACCATGTGCA+AGG - chr4.3:49884105-49884124 None:intergenic 40.0%
! AGATCACTGATGGATGTCAA+TGG + chr4.3:49883673-49883692 MS.gene66598:intron 40.0%
! CCAAAACTCTGAGGGATTAA+AGG - chr4.3:49884753-49884772 None:intergenic 40.0%
! CCTTTAATCCCTCAGAGTTT+TGG + chr4.3:49884750-49884769 MS.gene66598:CDS 40.0%
! GAAGAGAACTAAGAGTATGG+AGG + chr4.3:49881683-49881702 MS.gene66598:CDS 40.0%
! TCTAGGTTTTCTCATTGACG+AGG - chr4.3:49883402-49883421 None:intergenic 40.0%
! TGGAGGACCTTATATTGACT+AGG - chr4.3:49881254-49881273 None:intergenic 40.0%
!! AACTGATTTGCGTTCCTTTG+TGG - chr4.3:49884723-49884742 None:intergenic 40.0%
!! ACTTGGATTGGCTACTGATA+AGG + chr4.3:49883109-49883128 MS.gene66598:CDS 40.0%
!! GCCCATATTTTTGTGTACGT+GGG - chr4.3:49881882-49881901 None:intergenic 40.0%
!! GCTACCATTGCTATTTCCTT+CGG - chr4.3:49885039-49885058 None:intergenic 40.0%
AATGGCAACAATGTCACCGA+AGG + chr4.3:49885020-49885039 MS.gene66598:CDS 45.0%
ACAATGGCCATCGATGTGTT+AGG - chr4.3:49884228-49884247 None:intergenic 45.0%
ACTGGGGCCAAGGAAATTTA+TGG + chr4.3:49883085-49883104 MS.gene66598:CDS 45.0%
AGAGTGATGCAAGACCGTAA+GGG + chr4.3:49882990-49883009 MS.gene66598:CDS 45.0%
AGGCAGAGACAACTTCATGA+TGG - chr4.3:49881353-49881372 None:intergenic 45.0%
ATGATCATTGTCAGGGACAG+AGG - chr4.3:49881373-49881392 None:intergenic 45.0%
CAATGTGTGACACCCATACA+GGG + chr4.3:49881851-49881870 MS.gene66598:intron 45.0%
GCAGAAATGCATGACTCTCA+TGG - chr4.3:49882117-49882136 None:intergenic 45.0%
GGCCCACGTACACAAAAATA+TGG + chr4.3:49881877-49881896 MS.gene66598:intron 45.0%
GTCACCGAAGGAAATAGCAA+TGG + chr4.3:49885032-49885051 MS.gene66598:CDS 45.0%
GTGCTTATTACGTGAATGCC+TGG + chr4.3:49884306-49884325 MS.gene66598:intron 45.0%
TCAATGTGTGACACCCATAC+AGG + chr4.3:49881850-49881869 MS.gene66598:intron 45.0%
TGATATGCCTTGCACATGGT+TGG + chr4.3:49884095-49884114 MS.gene66598:intron 45.0%
TTACGCACCTAACACATCGA+TGG + chr4.3:49884218-49884237 MS.gene66598:intron 45.0%
! AACTGAGAATGGTGGAGTAC+TGG + chr4.3:49883067-49883086 MS.gene66598:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG + chr4.3:49883068-49883087 MS.gene66598:CDS 45.0%
! ACTTCAGAATGGTGGAGAGT+TGG + chr4.3:49883307-49883326 MS.gene66598:CDS 45.0%
! GCGTTCCTTTGTGGAGTATT+TGG - chr4.3:49884714-49884733 None:intergenic 45.0%
! GTATGGGTGTCACACATTGA+CGG - chr4.3:49881850-49881869 None:intergenic 45.0%
! TATGGGTGTCACACATTGAC+GGG - chr4.3:49881849-49881868 None:intergenic 45.0%
! TGGCATCAACAATGATGGGA+AGG + chr4.3:49885103-49885122 MS.gene66598:CDS 45.0%
!! GGCCCATATTTTTGTGTACG+TGG - chr4.3:49881883-49881902 None:intergenic 45.0%
!! GTTGGTGTCAAGGCAATGTA+AGG + chr4.3:49883325-49883344 MS.gene66598:CDS 45.0%
!! TGCTGGATTGGCTACTGATA+AGG + chr4.3:49883229-49883248 MS.gene66598:CDS 45.0%
!!! TTTTATATTTTTCTTTTATT+TGG + chr4.3:49882369-49882388 MS.gene66598:intron 5.0%
ACCTGCAGCAGCCAAAGAAA+TGG + chr4.3:49884515-49884534 MS.gene66598:CDS 50.0%
CTTTGGCTGCTGCAGGTAAA+GGG - chr4.3:49884512-49884531 None:intergenic 50.0%
GAGAGTGATGCAAGACCGTA+AGG + chr4.3:49882989-49883008 MS.gene66598:CDS 50.0%
TCTTTGGCTGCTGCAGGTAA+AGG - chr4.3:49884513-49884532 None:intergenic 50.0%
TTGAGGGATCAGAGAAGCTG+AGG - chr4.3:49884781-49884800 None:intergenic 50.0%
! GCAGCAGCAGTGTTGTTGTT+TGG - chr4.3:49884987-49885006 None:intergenic 50.0%
!! ATGGTGGAGAGTTGGTGTCA+AGG + chr4.3:49883315-49883334 MS.gene66598:CDS 50.0%
!! CTGAGAATGGTGGAGTACTG+GGG + chr4.3:49883069-49883088 MS.gene66598:CDS 50.0%
GCCATTTCTTTGGCTGCTGC+AGG - chr4.3:49884519-49884538 None:intergenic 55.0%
TGTGACACCCATACAGGGAC+AGG + chr4.3:49881856-49881875 MS.gene66598:intron 55.0%
! CTTTCGGACTGGGCAACCAT+GGG - chr4.3:49883766-49883785 None:intergenic 55.0%
ACGTGGGCCTGTCCCTGTAT+GGG - chr4.3:49881866-49881885 None:intergenic 60.0%
TACGTGGGCCTGTCCCTGTA+TGG - chr4.3:49881867-49881886 None:intergenic 60.0%
TATCGCTGCAGCACGACCCA+TGG + chr4.3:49883747-49883766 MS.gene66598:CDS 60.0%
TGGCTGCTGCAGGTAAAGGG+TGG - chr4.3:49884509-49884528 None:intergenic 60.0%
! GCTTTCGGACTGGGCAACCA+TGG - chr4.3:49883767-49883786 None:intergenic 60.0%
!! ATGGTGGAGTACTGGGGCCA+AGG + chr4.3:49883075-49883094 MS.gene66598:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 49881141 49885187 49881141 ID=MS.gene66598
chr4.3 mRNA 49881141 49885187 49881141 ID=MS.gene66598.t1;Parent=MS.gene66598
chr4.3 exon 49881141 49881434 49881141 ID=MS.gene66598.t1.exon1;Parent=MS.gene66598.t1
chr4.3 CDS 49881141 49881434 49881141 ID=cds.MS.gene66598.t1;Parent=MS.gene66598.t1
chr4.3 exon 49881603 49881704 49881603 ID=MS.gene66598.t1.exon2;Parent=MS.gene66598.t1
chr4.3 CDS 49881603 49881704 49881603 ID=cds.MS.gene66598.t1;Parent=MS.gene66598.t1
chr4.3 exon 49882879 49883574 49882879 ID=MS.gene66598.t1.exon3;Parent=MS.gene66598.t1
chr4.3 CDS 49882879 49883574 49882879 ID=cds.MS.gene66598.t1;Parent=MS.gene66598.t1
chr4.3 exon 49883675 49883788 49883675 ID=MS.gene66598.t1.exon4;Parent=MS.gene66598.t1
chr4.3 CDS 49883675 49883788 49883675 ID=cds.MS.gene66598.t1;Parent=MS.gene66598.t1
chr4.3 exon 49884447 49885187 49884447 ID=MS.gene66598.t1.exon5;Parent=MS.gene66598.t1
chr4.3 CDS 49884447 49885187 49884447 ID=cds.MS.gene66598.t1;Parent=MS.gene66598.t1
Gene Sequence

>MS.gene66598

ATGGCTTCAGTTCCAGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATAAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCTCATGAAACAAGAACAGAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGCCTCTGTCCCTGACAATGATCATATTCATACTGATACACCATCTTTATTGGAACTAAAGTTAAGCGTGAGTGACCATAATTCAATATCCCATTGTTATTATTTTTTTTACGTATTATATTATATATATTGTTTTTAAATTTCATAGCTTTTGAATCTGTAATCGCAAAAAAAAAAACATTTATTTTTTATGATTATCTTTTTAATTTGATGAATATTATTTTTTCGCATACAGCTTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGGTAATGTTATTTTTATTAAGACCTAGCAGAATTTAATGAAATTATATATGATGAACATTTTTTTTCCTTTAATTTTATTGAGTCAAAACTGGGTGATTTTATATATTGCATCGACACTCTTTAGATTTGAAGGTGTCTGGACCCGTCAATGTGTGACACCCATACAGGGACAGGCCCACGTACACAAAAATATGGGCCAAGGTCCTCGCTTTCTTTTAAATTTTTTCTACTATGTTATTTTTTTTGGAAAGAAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGATTCTTGGTTGGTTTGGCTTGTTAGTTTTTGTCTCTTACTCTTATATGACCTACATTTATAAGTATAATACAATATAATGTTATATGCAACCATGAGAGTCATGCATTTCTGCTCTTTATTTCAACCACAAATAATAATTATGTTATATTGAGTTAATTTAACTAGTATATTTTGTCAACGTACAATTATTTTATACATGCATCCAATGATATGCTGCCACGTTAATTAATGAATATGACAACTCATGTGTTTTCATATTTATTAATTGATGTAGCAGCACATCATTGAATGAATGTGTAAAATAATTGTACACCGACATAGTACGCTAATTAAATTCTTTCATATTTTATATTTTTCTTTTATTTGGTTTCATTTTTTAAAAGATACTAGTTTAATATTTTAAATGTAAATTGAATTTATTTACTCATTATTTTAAATGCTGCCCTCAATACTCAAATTTTCAGGGTCCGTCATTGCACCAATATGATCCTGACATAAACTGTTACATTTTGTTGTTTTATTTTCTCAAATTATTATTGGTTGTTGACGTGTCAGTTTTAATGTTTTGTCCGATTTTATATGTATGTCAGTGTTTATAGAATATTTTTGTGATTTGAATCCTCTTCTTTGTGAAAAAATTACTGAGTTATGTTAATGATCTCACTATTACAATGCATGGCCTTTTAGAACACTATTAACATGATGGGGTCCAATGTATTTTGATTTTGTTGGATAATCAAATGCAATTTAATAGTGAAATGATTATTATAACCCTAACATGTTTTATTCGAACGTGAGAGGATCCAAATCCGTTTTTTATGTGATTATATTTGAGTTCATTGAACTATTTTCAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCGGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAAGGAAATTTATGGAACTTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGACAAAAAGTTTCTGATCAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGTCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTCGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCAGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAAAGCTAGATCAGAAGAAACCATGGTAAGTATGTAAAAGTGTACATTAACTTTCAACTTTGCAAGTTTTTATATCATAATTTATTGTCCTTTTTACTAAAAAGAATTAATTTTTATTGAGTTAGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTAAGTTTCTTCTGTAAATATCCAAATTACATGTATTTACTAAAGTTTTAAATTTATGTCGCCCTATTGATTGCTTTCGCATTATTATATTTAATAGTATTATAGAGAATTATAGTCAAATATGATTAATGCAACGTCAATAACATTAAATTCGCAACGACATTAAACTCTTTGATTGTTGCCTAGATCATAGTCACAAACATGCATATTTTAAAACATTGGTCTTTAAATCATTATTTTATGTATGTTTTCAATTAATTAAAAATTAACTCCACATACAGAAACATTTTTAGTAATTGATTGATTTGATATGCCTTGCACATGGTTGGATAACAATATAATTTAATTGAAATTTGTTTCCACTTTCAAAGCTTATGTTTCTATGCTAGAAAATGGATTTGTATTTGCTCCATTCAGCAATCTATGTATTTACGCACCTAACACATCGATGGCCATTGTAAATCAAAATATGCAGTTATGTTTGGATCATCCAATCTTTGATACAACGACCATCAATGTGCTTATTACGTGAATGCCTGGATTTGCTGAATGCAATAAACTCAAATCCAAAATGGTAGCTACTTAGCTAGGTTAATTAAATTACTTATGAAGTGGTCTGTAATGTAATGACATATTTAATACAATTAAAACTTAACAGGTACAAAGGTGTGCTTTTGACAAAACAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGAAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACAGTCAATCTCCAAATACTCCACAAAGGAACGCAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAGTTAATGCTGCCATTGCTGCTAGTCCAAATATTCTTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCAGCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAACAAAATCAGGAATACCAATTACAAGACCTAAACTTTAATTTTTTTTGCTCAAAGATTTAG
Protein sequence

>MS.gene66598.t1

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHSTIPFLVNIRSSNENQTSPISSHETRTELDFFDDNNNDHHEVVSASVPDNDHIHTDTPSLLELKLSLGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMRKRFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVLGPRKFMELGLATDKVKEVKGQKVSDQKFVEKMRIKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELVSRQCKDLVLITNADIVMDHEASSSSMRKPRSQDQLGSAMKNIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIKARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCAFDKTVVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSASTSSNDGQLNENLLTRTLLPCSSSIATISASAPYPTITIDYSQSPNTPQRNANQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTVNAAIAASPNILAALSAAITSIIGDTQPNNNTAAADNNNNGNNVTEGNSNGSNNNVTEANSNKIITCGINNDGKEQNQEYQLQDLNFNFFCSKI