Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66703.t1 | XP_003606099.1 | 95.5 | 177 | 8 | 0 | 1 | 177 | 1 | 177 | 2.70E-90 | 341.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66703.t1 | Q3EAL1 | 50.0 | 84 | 41 | 1 | 77 | 159 | 43 | 126 | 8.5e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66703.t1 | G7JHA0 | 95.5 | 177 | 8 | 0 | 1 | 177 | 1 | 177 | 2.0e-90 | 341.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene66703.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66703.t1 | MTR_4g052040 | 95.480 | 177 | 8 | 0 | 1 | 177 | 1 | 177 | 9.06e-117 | 328 |
| MS.gene66703.t1 | MTR_4g052060 | 70.330 | 182 | 42 | 5 | 1 | 177 | 1 | 175 | 5.20e-80 | 235 |
| MS.gene66703.t1 | MTR_5g079710 | 46.988 | 166 | 76 | 5 | 4 | 168 | 6 | 160 | 1.21e-41 | 140 |
| MS.gene66703.t1 | MTR_7g105790 | 35.294 | 187 | 103 | 4 | 1 | 176 | 1 | 180 | 1.34e-26 | 100 |
| MS.gene66703.t1 | MTR_0907s0020 | 62.319 | 69 | 26 | 0 | 101 | 169 | 104 | 172 | 3.56e-25 | 96.3 |
| MS.gene66703.t1 | MTR_5g075210 | 57.377 | 61 | 26 | 0 | 102 | 162 | 283 | 343 | 4.37e-15 | 72.0 |
| MS.gene66703.t1 | MTR_8g103520 | 44.156 | 77 | 43 | 0 | 92 | 168 | 217 | 293 | 5.53e-15 | 71.2 |
| MS.gene66703.t1 | MTR_4g028930 | 49.180 | 61 | 31 | 0 | 100 | 160 | 315 | 375 | 6.85e-14 | 68.9 |
| MS.gene66703.t1 | MTR_7g072875 | 45.070 | 71 | 39 | 0 | 90 | 160 | 210 | 280 | 7.26e-14 | 68.2 |
| MS.gene66703.t1 | MTR_7g072630 | 45.070 | 71 | 39 | 0 | 90 | 160 | 272 | 342 | 1.02e-13 | 68.2 |
| MS.gene66703.t1 | MTR_2g018030 | 40.000 | 65 | 39 | 0 | 96 | 160 | 274 | 338 | 1.40e-11 | 62.0 |
| MS.gene66703.t1 | MTR_3g111780 | 46.835 | 79 | 42 | 0 | 86 | 164 | 185 | 263 | 2.96e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66703.t1 | AT3G52525 | 63.333 | 60 | 22 | 0 | 100 | 159 | 67 | 126 | 4.35e-22 | 87.4 |
| MS.gene66703.t1 | AT2G36026 | 57.377 | 61 | 26 | 0 | 104 | 164 | 91 | 151 | 1.17e-18 | 79.3 |
| MS.gene66703.t1 | AT5G19650 | 50.000 | 66 | 33 | 0 | 99 | 164 | 154 | 219 | 1.75e-15 | 71.6 |
| MS.gene66703.t1 | AT5G22240 | 44.595 | 74 | 39 | 1 | 100 | 171 | 97 | 170 | 1.06e-14 | 68.9 |
| MS.gene66703.t1 | AT1G06920 | 39.759 | 83 | 50 | 0 | 82 | 164 | 226 | 308 | 4.42e-14 | 68.9 |
| MS.gene66703.t1 | AT2G18500 | 46.875 | 64 | 34 | 0 | 97 | 160 | 224 | 287 | 9.10e-14 | 68.2 |
| MS.gene66703.t1 | AT5G58360 | 44.118 | 68 | 38 | 0 | 93 | 160 | 218 | 285 | 1.01e-13 | 67.8 |
| MS.gene66703.t1 | AT2G30400 | 46.875 | 64 | 34 | 0 | 97 | 160 | 250 | 313 | 3.80e-13 | 66.6 |
| MS.gene66703.t1 | AT5G01840 | 51.724 | 58 | 28 | 0 | 103 | 160 | 202 | 259 | 9.25e-13 | 65.1 |
| MS.gene66703.t1 | AT4G18830 | 46.552 | 58 | 31 | 0 | 102 | 159 | 285 | 342 | 2.87e-11 | 61.2 |
Find 34 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCATTAGTGGGCTTTGTT+TGG | 0.342399 | 4.3:+47856395 | None:intergenic |
| GCCGTGCTACCAACCTTGTC+GGG | 0.384391 | 4.3:+47856177 | None:intergenic |
| GAATGTTTGAAATCATGATA+TGG | 0.387193 | 4.3:+47856342 | None:intergenic |
| CTCAATTCTAGTTGTTATCA+TGG | 0.388006 | 4.3:-47856243 | MS.gene66703:CDS |
| AGCACGGCTATCAAACCTTC+CGG | 0.397168 | 4.3:-47856162 | MS.gene66703:CDS |
| TTCATTAGTGGGCTTTGTTT+GGG | 0.402789 | 4.3:+47856396 | None:intergenic |
| GATCACAAACCCAAGTAGTA+AGG | 0.415466 | 4.3:-47856535 | MS.gene66703:CDS |
| TTTCTCCACCACAACGTTCT+CGG | 0.421743 | 4.3:-47856448 | MS.gene66703:CDS |
| GGTTCTTCAACTACAACTTC+AGG | 0.425708 | 4.3:-47856513 | MS.gene66703:CDS |
| GTTGAAGAACCCTTACTACT+TGG | 0.435315 | 4.3:+47856525 | None:intergenic |
| ACAACTTCAGGTGAAGGATA+TGG | 0.438174 | 4.3:-47856501 | MS.gene66703:CDS |
| ACCGCCACCGTGTTCATTAG+TGG | 0.446501 | 4.3:+47856384 | None:intergenic |
| CGAGCCAAAGCCAACTCAAA+AGG | 0.483200 | 4.3:-47856580 | MS.gene66703:CDS |
| GCTGTCATCGATGCTACAAA+TGG | 0.491533 | 4.3:+47856476 | None:intergenic |
| TTCACATCAAATGGAAGTTG+TGG | 0.499142 | 4.3:-47856633 | MS.gene66703:CDS |
| GCACGGCTATCAAACCTTCC+GGG | 0.499919 | 4.3:-47856161 | MS.gene66703:CDS |
| ATGATCTTCAAGATCTTCTC+CGG | 0.511298 | 4.3:-47856278 | MS.gene66703:CDS |
| CCCCGACAAGGTTGGTAGCA+CGG | 0.514178 | 4.3:-47856178 | MS.gene66703:CDS |
| CCGCCACCGTGTTCATTAGT+GGG | 0.525048 | 4.3:+47856385 | None:intergenic |
| AGCCGTGCTACCAACCTTGT+CGG | 0.531475 | 4.3:+47856176 | None:intergenic |
| TTGAAGAACCCTTACTACTT+GGG | 0.531511 | 4.3:+47856526 | None:intergenic |
| GAACACGGTGGCGGTAGAGA+AGG | 0.563314 | 4.3:-47856376 | MS.gene66703:CDS |
| TCAACTACAACTTCAGGTGA+AGG | 0.579124 | 4.3:-47856507 | MS.gene66703:CDS |
| TCATGAAGCTTTCCCCGACA+AGG | 0.580331 | 4.3:-47856190 | MS.gene66703:CDS |
| GAAGCTTTCCCCGACAAGGT+TGG | 0.580453 | 4.3:-47856186 | MS.gene66703:CDS |
| ATCACAAACCCAAGTAGTAA+GGG | 0.581401 | 4.3:-47856534 | MS.gene66703:CDS |
| AATTGAGATGAAGAAAACAC+CGG | 0.628303 | 4.3:+47856259 | None:intergenic |
| AACATCCGAGAACGTTGTGG+TGG | 0.642922 | 4.3:+47856443 | None:intergenic |
| CCCACTAATGAACACGGTGG+CGG | 0.643057 | 4.3:-47856385 | MS.gene66703:CDS |
| CCGTGCTACCAACCTTGTCG+GGG | 0.653062 | 4.3:+47856178 | None:intergenic |
| GGCGGTAGAGAAGGACTCTG+AGG | 0.686815 | 4.3:-47856367 | MS.gene66703:CDS |
| ACAAAGCCCACTAATGAACA+CGG | 0.697529 | 4.3:-47856391 | MS.gene66703:CDS |
| GCTAACATCCGAGAACGTTG+TGG | 0.720216 | 4.3:+47856440 | None:intergenic |
| AAGCCCACTAATGAACACGG+TGG | 0.804493 | 4.3:-47856388 | MS.gene66703:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTGAAAAATGTTCTTATA+AGG | + | chr4.3:47856181-47856200 | None:intergenic | 20.0% |
| !! | AGAACATTTTTCACATCAAA+TGG | - | chr4.3:47856185-47856204 | MS.gene66703:CDS | 25.0% |
| !! | GAATGTTTGAAATCATGATA+TGG | + | chr4.3:47856488-47856507 | None:intergenic | 25.0% |
| !! | TCTTATAAGGTTTTTCTTGT+TGG | + | chr4.3:47856168-47856187 | None:intergenic | 25.0% |
| !!! | GTTTGTGATCTTTTGATTAA+TGG | + | chr4.3:47856282-47856301 | None:intergenic | 25.0% |
| AATTGAGATGAAGAAAACAC+CGG | + | chr4.3:47856571-47856590 | None:intergenic | 30.0% | |
| ! | CTCAATTCTAGTTGTTATCA+TGG | - | chr4.3:47856584-47856603 | MS.gene66703:CDS | 30.0% |
| !! | GAAAATTTTGCCTTTTGAGT+TGG | + | chr4.3:47856260-47856279 | None:intergenic | 30.0% |
| ATCACAAACCCAAGTAGTAA+GGG | - | chr4.3:47856293-47856312 | MS.gene66703:CDS | 35.0% | |
| ATGATCTTCAAGATCTTCTC+CGG | - | chr4.3:47856549-47856568 | MS.gene66703:CDS | 35.0% | |
| TTCACATCAAATGGAAGTTG+TGG | - | chr4.3:47856194-47856213 | MS.gene66703:CDS | 35.0% | |
| TTGAAGAACCCTTACTACTT+GGG | + | chr4.3:47856304-47856323 | None:intergenic | 35.0% | |
| !! | TTCATTAGTGGGCTTTGTTT+GGG | + | chr4.3:47856434-47856453 | None:intergenic | 35.0% |
| ACAAAGCCCACTAATGAACA+CGG | - | chr4.3:47856436-47856455 | MS.gene66703:CDS | 40.0% | |
| ACAACTTCAGGTGAAGGATA+TGG | - | chr4.3:47856326-47856345 | MS.gene66703:CDS | 40.0% | |
| GATCACAAACCCAAGTAGTA+AGG | - | chr4.3:47856292-47856311 | MS.gene66703:CDS | 40.0% | |
| GGTTCTTCAACTACAACTTC+AGG | - | chr4.3:47856314-47856333 | MS.gene66703:CDS | 40.0% | |
| GTTGAAGAACCCTTACTACT+TGG | + | chr4.3:47856305-47856324 | None:intergenic | 40.0% | |
| TCAACTACAACTTCAGGTGA+AGG | - | chr4.3:47856320-47856339 | MS.gene66703:CDS | 40.0% | |
| !! | GTTCATTAGTGGGCTTTGTT+TGG | + | chr4.3:47856435-47856454 | None:intergenic | 40.0% |
| !!! | TTTGCCTTTTGAGTTGGCTT+TGG | + | chr4.3:47856254-47856273 | None:intergenic | 40.0% |
| GCTGTCATCGATGCTACAAA+TGG | + | chr4.3:47856354-47856373 | None:intergenic | 45.0% | |
| TTTCTCCACCACAACGTTCT+CGG | - | chr4.3:47856379-47856398 | MS.gene66703:CDS | 45.0% | |
| AACATCCGAGAACGTTGTGG+TGG | + | chr4.3:47856387-47856406 | None:intergenic | 50.0% | |
| AAGCCCACTAATGAACACGG+TGG | - | chr4.3:47856439-47856458 | MS.gene66703:CDS | 50.0% | |
| AGCACGGCTATCAAACCTTC+CGG | - | chr4.3:47856665-47856684 | MS.gene66703:CDS | 50.0% | |
| CGAGCCAAAGCCAACTCAAA+AGG | - | chr4.3:47856247-47856266 | MS.gene66703:CDS | 50.0% | |
| GCTAACATCCGAGAACGTTG+TGG | + | chr4.3:47856390-47856409 | None:intergenic | 50.0% | |
| TCATGAAGCTTTCCCCGACA+AGG | - | chr4.3:47856637-47856656 | MS.gene66703:CDS | 50.0% | |
| ACCGCCACCGTGTTCATTAG+TGG | + | chr4.3:47856446-47856465 | None:intergenic | 55.0% | |
| AGCCGTGCTACCAACCTTGT+CGG | + | chr4.3:47856654-47856673 | None:intergenic | 55.0% | |
| CCCACTAATGAACACGGTGG+CGG | - | chr4.3:47856442-47856461 | MS.gene66703:CDS | 55.0% | |
| CCGCCACCGTGTTCATTAGT+GGG | + | chr4.3:47856445-47856464 | None:intergenic | 55.0% | |
| GAAGCTTTCCCCGACAAGGT+TGG | - | chr4.3:47856641-47856660 | MS.gene66703:CDS | 55.0% | |
| GCACGGCTATCAAACCTTCC+GGG | - | chr4.3:47856666-47856685 | MS.gene66703:CDS | 55.0% | |
| CCGTGCTACCAACCTTGTCG+GGG | + | chr4.3:47856652-47856671 | None:intergenic | 60.0% | |
| GCCGTGCTACCAACCTTGTC+GGG | + | chr4.3:47856653-47856672 | None:intergenic | 60.0% | |
| GGCGGTAGAGAAGGACTCTG+AGG | - | chr4.3:47856460-47856479 | MS.gene66703:CDS | 60.0% | |
| ! | GAACACGGTGGCGGTAGAGA+AGG | - | chr4.3:47856451-47856470 | MS.gene66703:CDS | 60.0% |
| !! | CCCCGACAAGGTTGGTAGCA+CGG | - | chr4.3:47856649-47856668 | MS.gene66703:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 47856158 | 47856691 | 47856158 | ID=MS.gene66703 |
| chr4.3 | mRNA | 47856158 | 47856691 | 47856158 | ID=MS.gene66703.t1;Parent=MS.gene66703 |
| chr4.3 | exon | 47856158 | 47856691 | 47856158 | ID=MS.gene66703.t1.exon1;Parent=MS.gene66703.t1 |
| chr4.3 | CDS | 47856158 | 47856691 | 47856158 | ID=cds.MS.gene66703.t1;Parent=MS.gene66703.t1 |
| Gene Sequence |
| Protein sequence |
