Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67165.t1 | XP_013467071.1 | 86.9 | 198 | 26 | 0 | 1 | 198 | 1 | 198 | 4.60E-54 | 221.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67165.t1 | Q9FJW5 | 70.8 | 65 | 19 | 0 | 1 | 65 | 1 | 65 | 1.7e-20 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67165.t1 | A0A072VHK1 | 86.9 | 198 | 26 | 0 | 1 | 198 | 1 | 198 | 3.3e-54 | 221.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene67165.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67165.t1 | MTR_1g041765 | 87.879 | 198 | 24 | 0 | 1 | 198 | 1 | 198 | 1.30e-109 | 312 |
| MS.gene67165.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 5.61e-35 | 125 |
| MS.gene67165.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 5.61e-35 | 125 |
| MS.gene67165.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 5.59e-34 | 124 |
| MS.gene67165.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.13e-33 | 121 |
| MS.gene67165.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.47e-33 | 121 |
| MS.gene67165.t1 | MTR_4g097130 | 65.169 | 89 | 30 | 1 | 1 | 88 | 1 | 89 | 2.71e-33 | 121 |
| MS.gene67165.t1 | MTR_0095s0040 | 58.511 | 94 | 35 | 2 | 1 | 90 | 1 | 94 | 2.91e-28 | 106 |
| MS.gene67165.t1 | MTR_8g494240 | 58.511 | 94 | 35 | 2 | 1 | 90 | 1 | 94 | 9.00e-28 | 106 |
| MS.gene67165.t1 | MTR_8g494240 | 58.511 | 94 | 35 | 2 | 1 | 90 | 1 | 94 | 9.68e-28 | 106 |
| MS.gene67165.t1 | MTR_8g494240 | 58.511 | 94 | 35 | 2 | 1 | 90 | 1 | 94 | 4.51e-27 | 106 |
| MS.gene67165.t1 | MTR_2g031870 | 70.588 | 68 | 19 | 1 | 1 | 67 | 1 | 68 | 5.79e-27 | 104 |
| MS.gene67165.t1 | MTR_8g092810 | 70.690 | 58 | 17 | 0 | 1 | 58 | 1 | 58 | 1.01e-23 | 97.8 |
| MS.gene67165.t1 | MTR_2g099610 | 65.517 | 58 | 20 | 0 | 1 | 58 | 1 | 58 | 7.69e-21 | 88.2 |
| MS.gene67165.t1 | MTR_8g018410 | 42.574 | 101 | 51 | 1 | 1 | 101 | 1 | 94 | 1.25e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67165.t1 | AT5G67580 | 63.529 | 85 | 29 | 1 | 1 | 83 | 1 | 85 | 3.91e-29 | 110 |
| MS.gene67165.t1 | AT5G67580 | 63.529 | 85 | 29 | 1 | 1 | 83 | 1 | 85 | 3.91e-29 | 110 |
| MS.gene67165.t1 | AT3G49850 | 54.348 | 92 | 42 | 0 | 1 | 92 | 1 | 92 | 2.96e-27 | 105 |
| MS.gene67165.t1 | AT3G49850 | 54.348 | 92 | 42 | 0 | 1 | 92 | 1 | 92 | 2.96e-27 | 105 |
| MS.gene67165.t1 | AT1G49950 | 68.116 | 69 | 21 | 1 | 1 | 68 | 1 | 69 | 3.20e-26 | 102 |
| MS.gene67165.t1 | AT1G49950 | 68.116 | 69 | 21 | 1 | 1 | 68 | 1 | 69 | 3.20e-26 | 102 |
| MS.gene67165.t1 | AT1G49950 | 68.116 | 69 | 21 | 1 | 1 | 68 | 1 | 69 | 3.20e-26 | 102 |
| MS.gene67165.t1 | AT1G72740 | 59.375 | 64 | 26 | 0 | 1 | 64 | 1 | 64 | 4.37e-20 | 85.9 |
| MS.gene67165.t1 | AT1G72740 | 63.793 | 58 | 21 | 0 | 1 | 58 | 1 | 58 | 5.06e-20 | 85.5 |
| MS.gene67165.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 7.32e-20 | 85.1 |
| MS.gene67165.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 7.40e-20 | 85.5 |
| MS.gene67165.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 7.42e-20 | 85.1 |
| MS.gene67165.t1 | AT1G17520 | 57.576 | 66 | 28 | 0 | 1 | 66 | 1 | 66 | 8.00e-20 | 85.1 |
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAAACTAGTGTTGAATTC+TGG | 0.175108 | 1.2:+26160228 | None:intergenic |
| TTATTTCTCTCGGTTGCTTT+TGG | 0.246977 | 1.2:+26159997 | None:intergenic |
| GCAAACTAGTGTTGAATTCT+GGG | 0.405933 | 1.2:+26160229 | None:intergenic |
| CAGAGCTGCCTCTTCTTCTC+CGG | 0.417460 | 1.2:+26160305 | None:intergenic |
| GAGGGGTCTCACACCTCAAC+AGG | 0.429564 | 1.2:-26159917 | MS.gene67165:CDS |
| GAATATCAATATTCGATCAT+AGG | 0.430998 | 1.2:-26160068 | MS.gene67165:CDS |
| ATGTAGACCTCAAGGACAAA+TGG | 0.452440 | 1.2:-26160194 | MS.gene67165:CDS |
| GAAGAGGCAGCTCTGAAAGC+GGG | 0.486977 | 1.2:-26160297 | MS.gene67165:CDS |
| GTTGACATCATTATTTCTCT+CGG | 0.490773 | 1.2:+26159987 | None:intergenic |
| GGCAGAAGCTGCCATGACTA+AGG | 0.496803 | 1.2:-26159863 | MS.gene67165:CDS |
| GGAGTAGTCAAACACGGAGC+TGG | 0.501232 | 1.2:-26160276 | MS.gene67165:CDS |
| AGTTCCTCCATTTGTCCTTG+AGG | 0.505844 | 1.2:+26160187 | None:intergenic |
| TCAAACACGGAGCTGGAAGA+TGG | 0.524401 | 1.2:-26160269 | MS.gene67165:CDS |
| TGCTTCCTCAGCCTTAGTCA+TGG | 0.542408 | 1.2:+26159852 | None:intergenic |
| TGACGACAACATTTAGATCA+AGG | 0.547610 | 1.2:-26160158 | MS.gene67165:CDS |
| AATAATAATCAATATTCAAG+TGG | 0.553519 | 1.2:-26160090 | MS.gene67165:CDS |
| AAAATGGACCGGAGAAGAAG+AGG | 0.564638 | 1.2:-26160313 | MS.gene67165:CDS |
| AGAAGAAGTTGCAAAAGCAA+TGG | 0.568319 | 1.2:-26159890 | MS.gene67165:CDS |
| AGTTGCAAAAGCAATGGAGA+AGG | 0.571478 | 1.2:-26159884 | MS.gene67165:CDS |
| AGCTGCCATGACTAAGGCTG+AGG | 0.573794 | 1.2:-26159857 | MS.gene67165:CDS |
| ACTTCTTCTATCGCCTGTTG+AGG | 0.575155 | 1.2:+26159904 | None:intergenic |
| TGCAAAAGCAGAAATGAAGA+AGG | 0.586897 | 1.2:-26159779 | MS.gene67165:CDS |
| GGAAGCAACTAATGAGGCAG+AGG | 0.590847 | 1.2:-26159836 | MS.gene67165:CDS |
| TCGTTCAAATGTAGACCTCA+AGG | 0.605301 | 1.2:-26160202 | MS.gene67165:CDS |
| GGCTGAGGAAGCAACTAATG+AGG | 0.610331 | 1.2:-26159842 | MS.gene67165:CDS |
| AGAAGAGGCAGCTCTGAAAG+CGG | 0.618500 | 1.2:-26160298 | MS.gene67165:CDS |
| TAGACCTCAAGGACAAATGG+AGG | 0.657376 | 1.2:-26160191 | MS.gene67165:CDS |
| AAAGCGGGAGTAGTCAAACA+CGG | 0.721145 | 1.2:-26160282 | MS.gene67165:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATCAATATTCAAG+TGG | - | chr1.2:26159996-26160015 | MS.gene67165:CDS | 15.0% |
| !!! | TGATTATTATTTTCAACTTT+AGG | + | chr1.2:26159987-26160006 | None:intergenic | 15.0% |
| !!! | AAGAGATTTTTAAAATGATG+AGG | - | chr1.2:26160150-26160169 | MS.gene67165:CDS | 20.0% |
| !!! | AGAGATTTTTAAAATGATGA+GGG | - | chr1.2:26160151-26160170 | MS.gene67165:CDS | 20.0% |
| !!! | TAATTTCTTCGTATTTTGAA+AGG | + | chr1.2:26160127-26160146 | None:intergenic | 20.0% |
| !!! | TTATTATTTTCAACTTTAGG+TGG | + | chr1.2:26159984-26160003 | None:intergenic | 20.0% |
| ! | GAATATCAATATTCGATCAT+AGG | - | chr1.2:26160018-26160037 | MS.gene67165:CDS | 25.0% |
| !!! | GAGATTTTTAAAATGATGAG+GGG | - | chr1.2:26160152-26160171 | MS.gene67165:CDS | 25.0% |
| ! | GTTGACATCATTATTTCTCT+CGG | + | chr1.2:26160102-26160121 | None:intergenic | 30.0% |
| ! | TTATTTTCAACTTTAGGTGG+AGG | + | chr1.2:26159981-26160000 | None:intergenic | 30.0% |
| AACTTCAACAAAAATACGCG+AGG | - | chr1.2:26160049-26160068 | MS.gene67165:CDS | 35.0% | |
| AGAAGAAGTTGCAAAAGCAA+TGG | - | chr1.2:26160196-26160215 | MS.gene67165:CDS | 35.0% | |
| TGACGACAACATTTAGATCA+AGG | - | chr1.2:26159928-26159947 | MS.gene67165:CDS | 35.0% | |
| TGCAAAAGCAGAAATGAAGA+AGG | - | chr1.2:26160307-26160326 | MS.gene67165:CDS | 35.0% | |
| ! | GCAAACTAGTGTTGAATTCT+GGG | + | chr1.2:26159860-26159879 | None:intergenic | 35.0% |
| !! | GCGTATTTTTGTTGAAGTTG+AGG | + | chr1.2:26160048-26160067 | None:intergenic | 35.0% |
| !! | TTATTTCTCTCGGTTGCTTT+TGG | + | chr1.2:26160092-26160111 | None:intergenic | 35.0% |
| AGTTGCAAAAGCAATGGAGA+AGG | - | chr1.2:26160202-26160221 | MS.gene67165:CDS | 40.0% | |
| ATGTAGACCTCAAGGACAAA+TGG | - | chr1.2:26159892-26159911 | MS.gene67165:CDS | 40.0% | |
| TCGTTCAAATGTAGACCTCA+AGG | - | chr1.2:26159884-26159903 | MS.gene67165:CDS | 40.0% | |
| ! | CGCAAACTAGTGTTGAATTC+TGG | + | chr1.2:26159861-26159880 | None:intergenic | 40.0% |
| AAAATGGACCGGAGAAGAAG+AGG | - | chr1.2:26159773-26159792 | MS.gene67165:CDS | 45.0% | |
| AAAGCGGGAGTAGTCAAACA+CGG | - | chr1.2:26159804-26159823 | MS.gene67165:CDS | 45.0% | |
| ACTTCTTCTATCGCCTGTTG+AGG | + | chr1.2:26160185-26160204 | None:intergenic | 45.0% | |
| AGTTCCTCCATTTGTCCTTG+AGG | + | chr1.2:26159902-26159921 | None:intergenic | 45.0% | |
| GCTCCTAAGCAAAAATGGAC+CGG | - | chr1.2:26159762-26159781 | MS.gene67165:CDS | 45.0% | |
| TAGACCTCAAGGACAAATGG+AGG | - | chr1.2:26159895-26159914 | MS.gene67165:CDS | 45.0% | |
| TGGGTGCTCCTAAGCAAAAA+TGG | - | chr1.2:26159757-26159776 | MS.gene67165:CDS | 45.0% | |
| !! | TCTCCGGTCCATTTTTGCTT+AGG | + | chr1.2:26159768-26159787 | None:intergenic | 45.0% |
| GGAAGCAACTAATGAGGCAG+AGG | - | chr1.2:26160250-26160269 | MS.gene67165:CDS | 50.0% | |
| GGCTGAGGAAGCAACTAATG+AGG | - | chr1.2:26160244-26160263 | MS.gene67165:CDS | 50.0% | |
| TCAAACACGGAGCTGGAAGA+TGG | - | chr1.2:26159817-26159836 | MS.gene67165:CDS | 50.0% | |
| TGCTTCCTCAGCCTTAGTCA+TGG | + | chr1.2:26160237-26160256 | None:intergenic | 50.0% | |
| !! | AGAAGAGGCAGCTCTGAAAG+CGG | - | chr1.2:26159788-26159807 | MS.gene67165:CDS | 50.0% |
| AGCTGCCATGACTAAGGCTG+AGG | - | chr1.2:26160229-26160248 | MS.gene67165:CDS | 55.0% | |
| CAGAGCTGCCTCTTCTTCTC+CGG | + | chr1.2:26159784-26159803 | None:intergenic | 55.0% | |
| GGAGTAGTCAAACACGGAGC+TGG | - | chr1.2:26159810-26159829 | MS.gene67165:CDS | 55.0% | |
| GGCAGAAGCTGCCATGACTA+AGG | - | chr1.2:26160223-26160242 | MS.gene67165:CDS | 55.0% | |
| !! | GAAGAGGCAGCTCTGAAAGC+GGG | - | chr1.2:26159789-26159808 | MS.gene67165:CDS | 55.0% |
| GAGGGGTCTCACACCTCAAC+AGG | - | chr1.2:26160169-26160188 | MS.gene67165:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 26159756 | 26160352 | 26159756 | ID=MS.gene67165 |
| chr1.2 | mRNA | 26159756 | 26160352 | 26159756 | ID=MS.gene67165.t1;Parent=MS.gene67165 |
| chr1.2 | exon | 26159756 | 26160352 | 26159756 | ID=MS.gene67165.t1.exon1;Parent=MS.gene67165.t1 |
| chr1.2 | CDS | 26159756 | 26160352 | 26159756 | ID=cds.MS.gene67165.t1;Parent=MS.gene67165.t1 |
| Gene Sequence |
| Protein sequence |
