AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67274.t1 MTR_5g073430 86.047 86 12 0 91 176 1 86 4.80e-50 157
MS.gene67274.t1 MTR_7g083950 62.025 79 30 0 91 169 2 80 1.51e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCATCAGGGTTTATTATT+CGG 0.287310 5.1:+56644751 MS.gene67274:CDS
CGTTCTCTTTCTTGCCGGCT+TGG 0.354018 5.1:-56642649 None:intergenic
GCTGGAAGAATTGATATAAT+AGG 0.357954 5.1:+56644697 MS.gene67274:CDS
CGACTAAGGCGCCCGCAAAA+AGG 0.377083 5.1:+56642670 MS.gene67274:CDS
GCTTCTCGCTGGATCGGTTC+GGG 0.377332 5.1:-56642747 None:intergenic
TGCACTGGAGTATCTTCTTA+AGG 0.379025 5.1:+56644600 MS.gene67274:CDS
TGCTTCTCGCTGGATCGGTT+CGG 0.381666 5.1:-56642748 None:intergenic
TGGGATGCTTCAAAGGCATC+AGG 0.403391 5.1:+56644738 MS.gene67274:CDS
AAGGCAAGAGGAGATCAAAA+TGG 0.409826 5.1:+56642811 MS.gene67274:CDS
AAATGACATTCTTCTTCAAC+AGG 0.416029 5.1:-56642720 None:intergenic
TCAGCAGGTGTTTCCTTTGG+TGG 0.418829 5.1:-56642852 None:intergenic
GATACTCCAGTGCATGTTCA+TGG 0.422585 5.1:-56644591 None:intergenic
TTAGTCGTTCTCTTTCTTGC+CGG 0.427927 5.1:-56642654 None:intergenic
GCATCCCACTAACTCTTGAT+TGG 0.437559 5.1:-56644723 None:intergenic
GCACTGGAGTATCTTCTTAA+GGG 0.471379 5.1:+56644601 MS.gene67274:CDS
GGCAAGAAAGAGAACGACTA+AGG 0.474278 5.1:+56642656 MS.gene67274:CDS
AAAACTCACAAGAGATTACA+AGG 0.479131 5.1:+56644675 MS.gene67274:CDS
ACCTTCCTCATCATGCCAGC+TGG 0.479240 5.1:+56644781 MS.gene67274:CDS
AACAGGAGTAGTATGTTGTT+CGG 0.484971 5.1:-56642703 None:intergenic
CGATCAAGAGTGCTTCTCGC+TGG 0.496655 5.1:-56642758 None:intergenic
CTCACAAGAGATTACAAGGC+TGG 0.498781 5.1:+56644679 MS.gene67274:CDS
GCTGGTTTGAACTCTTTCCA+TGG 0.521060 5.1:+56644799 MS.gene67274:CDS
GAGTTAGTGGGATGCTTCAA+AGG 0.526784 5.1:+56644731 MS.gene67274:CDS
TCGATGTGCACGCTAGATGT+AGG 0.527235 5.1:+56642778 MS.gene67274:CDS
AAGAGTGCTTCTCGCTGGAT+CGG 0.534745 5.1:-56642753 None:intergenic
GGGAGGAGTATGAAGGCAAG+AGG 0.535714 5.1:+56642799 MS.gene67274:CDS
CGATGTGCACGCTAGATGTA+GGG 0.538824 5.1:+56642779 MS.gene67274:CDS
GGCATCCAATCAAGAGTTAG+TGG 0.541035 5.1:+56644718 MS.gene67274:CDS
AGAGGAGATCAAAATGGAAA+AGG 0.542153 5.1:+56642817 MS.gene67274:CDS
TTATTGCCATGAACATGCAC+TGG 0.547671 5.1:+56644585 MS.gene67274:CDS
GATGAGGCATGGAGCCAAGC+CGG 0.548007 5.1:+56642635 None:intergenic
TGGAAAGAGTTCAAACCAGC+TGG 0.553560 5.1:-56644796 None:intergenic
TGTTCATGGCAATAATTTCG+AGG 0.557832 5.1:-56644577 None:intergenic
AGATGTAGGGAGGAGTATGA+AGG 0.589462 5.1:+56642792 MS.gene67274:CDS
GGGATGCTTCAAAGGCATCA+GGG 0.590682 5.1:+56644739 MS.gene67274:CDS
TCAAACCAGCTGGCATGATG+AGG 0.594872 5.1:-56644786 None:intergenic
ATTATGTGAATTACGTACCA+TGG 0.602512 5.1:-56644816 None:intergenic
GAGTGTTAATGAACCACCAA+AGG 0.606018 5.1:+56642839 MS.gene67274:CDS
GTTCATGGCAATAATTTCGA+GGG 0.608298 5.1:-56644576 None:intergenic
ACCAGCTGGCATGATGAGGA+AGG 0.612834 5.1:-56644782 None:intergenic
AGGGAGTGAAACTTGCATCT+CGG 0.621205 5.1:+56644620 MS.gene67274:CDS
GCATCCAATCAAGAGTTAGT+GGG 0.623914 5.1:+56644719 MS.gene67274:CDS
CCAGCTCTAAGGATGAGGCA+TGG 0.647183 5.1:+56642624 None:intergenic
TGTGCACGCTAGATGTAGGG+AGG 0.691146 5.1:+56642782 MS.gene67274:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTGTTTAATAGATTA+AGG + chr5.1:56643569-56643588 MS.gene67274:intron 10.0%
!!! TTTTTAATTATAGTAGAAAT+AGG - chr5.1:56643406-56643425 None:intergenic 10.0%
!! AATAAAATAAAAAAACGTTC+TGG - chr5.1:56643290-56643309 None:intergenic 15.0%
!! TCTATAATAAATATTGTTGT+TGG - chr5.1:56644113-56644132 None:intergenic 15.0%
!!! ACTACTACTAAATTTTTTTT+TGG - chr5.1:56643615-56643634 None:intergenic 15.0%
!!! TATAGATTTTAAAAGATTGT+CGG + chr5.1:56643513-56643532 MS.gene67274:intron 15.0%
!!! TTACACTTTTTAAATGATAA+AGG + chr5.1:56644203-56644222 MS.gene67274:intron 15.0%
!!! TTGGTTTTATTATTTTGAAT+TGG + chr5.1:56644503-56644522 MS.gene67274:intron 15.0%
!! AAAGATTAAACTCAATTTAC+TGG - chr5.1:56643317-56643336 None:intergenic 20.0%
!! CATTGAAGATATATATTCAA+AGG - chr5.1:56642935-56642954 None:intergenic 20.0%
!! TATATAAGTAGCAAAAAGAT+GGG + chr5.1:56643854-56643873 MS.gene67274:intron 20.0%
!! TTATATAAGTAGCAAAAAGA+TGG + chr5.1:56643853-56643872 MS.gene67274:intron 20.0%
!! TTATTTGTATTGAAAGTTGA+GGG + chr5.1:56644301-56644320 MS.gene67274:intron 20.0%
!!! AGTAGTAGTTTTTTTTTTGA+AGG + chr5.1:56643626-56643645 MS.gene67274:intron 20.0%
!!! TGTTGTTTAAGAGTATAATT+GGG + chr5.1:56644141-56644160 MS.gene67274:intron 20.0%
!!! TTGTTGTTTAAGAGTATAAT+TGG + chr5.1:56644140-56644159 MS.gene67274:intron 20.0%
!!! TTTTTTGTTTTTACGTGAAA+CGG + chr5.1:56643067-56643086 MS.gene67274:intron 20.0%
! ATAATCCCTCAAATCTATTA+TGG + chr5.1:56643116-56643135 MS.gene67274:intron 25.0%
! ATCATCTGTAGTAATAAGTA+CGG + chr5.1:56643019-56643038 MS.gene67274:intron 25.0%
! GTTATTTGTATTGAAAGTTG+AGG + chr5.1:56644300-56644319 MS.gene67274:intron 25.0%
! TAATCCCTCAAATCTATTAT+GGG + chr5.1:56643117-56643136 MS.gene67274:intron 25.0%
!! AAAGTGATAAGTTTGAGATT+TGG - chr5.1:56643761-56643780 None:intergenic 25.0%
!!! AAAATAACGTACTCTGAAAT+AGG + chr5.1:56644411-56644430 MS.gene67274:intron 25.0%
!!! AAATAACGTACTCTGAAATA+GGG + chr5.1:56644412-56644431 MS.gene67274:intron 25.0%
!!! AGAGTACGTTATTTTTTTAG+GGG - chr5.1:56644406-56644425 None:intergenic 25.0%
!!! CAGAGTACGTTATTTTTTTA+GGG - chr5.1:56644407-56644426 None:intergenic 25.0%
!!! CTAGAATCGTTAGTTTTTTT+TGG + chr5.1:56644484-56644503 MS.gene67274:intron 25.0%
!!! TCAGAGTACGTTATTTTTTT+AGG - chr5.1:56644408-56644427 None:intergenic 25.0%
!!! TTGTTTTCGACTTTTTTTGA+AGG + chr5.1:56643429-56643448 MS.gene67274:intron 25.0%
!!! TTTTTGTTTTTACGTGAAAC+GGG + chr5.1:56643068-56643087 MS.gene67274:intron 25.0%
AAAACTCACAAGAGATTACA+AGG + chr5.1:56644675-56644694 MS.gene67274:CDS 30.0%
AAACAAATCAAGGAGGAATA+TGG + chr5.1:56643221-56643240 MS.gene67274:intron 30.0%
AAATGACATTCTTCTTCAAC+AGG - chr5.1:56642723-56642742 None:intergenic 30.0%
GCTGGAAGAATTGATATAAT+AGG + chr5.1:56644697-56644716 MS.gene67274:CDS 30.0%
GTTAAACCCAAAACAAATCA+AGG + chr5.1:56643211-56643230 MS.gene67274:intron 30.0%
TAGATTTGAGGGATTATTCT+AGG - chr5.1:56643113-56643132 None:intergenic 30.0%
! AGGAACGATTTTACAATAAG+AGG - chr5.1:56643386-56643405 None:intergenic 30.0%
! AGTTTGAGATTTGGATTTGA+AGG - chr5.1:56643752-56643771 None:intergenic 30.0%
! ATTGGAATTTGTAGTCTTAC+TGG + chr5.1:56644521-56644540 MS.gene67274:intron 30.0%
! TTCTAGGAAGTTGAACATAA+AGG - chr5.1:56643097-56643116 None:intergenic 30.0%
!! GATTTTAAAAGATTGTCGGA+AGG + chr5.1:56643517-56643536 MS.gene67274:intron 30.0%
!!! ATTCCTCCTTGATTTGTTTT+GGG - chr5.1:56643220-56643239 None:intergenic 30.0%
!!! TATTCCTCCTTGATTTGTTT+TGG - chr5.1:56643221-56643240 None:intergenic 30.0%
!!! TGTTTTGGGTTTAACGATTT+CGG - chr5.1:56643206-56643225 None:intergenic 30.0%
!!! TTGAAAGTTGAGGGTATTTT+TGG + chr5.1:56644310-56644329 MS.gene67274:intron 30.0%
AAACCCAAAACAAATCAAGG+AGG + chr5.1:56643214-56643233 MS.gene67274:intron 35.0%
AACAGGAGTAGTATGTTGTT+CGG - chr5.1:56642706-56642725 None:intergenic 35.0%
AATCAAGGAGGAATATGGTA+TGG + chr5.1:56643226-56643245 MS.gene67274:intron 35.0%
ACTAACGATTCTAGTGAAAG+AGG - chr5.1:56644478-56644497 None:intergenic 35.0%
AGAGGAGATCAAAATGGAAA+AGG + chr5.1:56642817-56642836 MS.gene67274:CDS 35.0%
AGGCATCAGGGTTTATTATT+CGG + chr5.1:56644751-56644770 MS.gene67274:CDS 35.0%
ATGTGTGACATGATCTGATA+TGG + chr5.1:56642952-56642971 MS.gene67274:intron 35.0%
GACAAGTAGTAGTAGTTGTA+TGG + chr5.1:56643674-56643693 MS.gene67274:intron 35.0%
GGATTTGAAGGAGTGAAATT+CGG - chr5.1:56643740-56643759 None:intergenic 35.0%
GTTCATGGCAATAATTTCGA+GGG - chr5.1:56644579-56644598 None:intergenic 35.0%
TGGTCCCATAATAGATTTGA+GGG - chr5.1:56643124-56643143 None:intergenic 35.0%
TGTTCATGGCAATAATTTCG+AGG - chr5.1:56644580-56644599 None:intergenic 35.0%
! ACTATCCATTTCAGCGTTTT+TGG - chr5.1:56644352-56644371 None:intergenic 35.0%
! CTATCCATTTCAGCGTTTTT+GGG - chr5.1:56644351-56644370 None:intergenic 35.0%
!! GAGAGTTTTGTCAATCATTG+TGG - chr5.1:56643144-56643163 None:intergenic 35.0%
AAAGAGGATTCAGAAGCTAC+GGG - chr5.1:56644462-56644481 None:intergenic 40.0%
AAGGCAAGAGGAGATCAAAA+TGG + chr5.1:56642811-56642830 MS.gene67274:CDS 40.0%
AGAAGCTACGGGAATAGAAA+CGG - chr5.1:56644451-56644470 None:intergenic 40.0%
CGTTCTGGCGATGAAAAAAA+AGG - chr5.1:56643275-56643294 None:intergenic 40.0%
GAAAGAGGATTCAGAAGCTA+CGG - chr5.1:56644463-56644482 None:intergenic 40.0%
GAGTGTTAATGAACCACCAA+AGG + chr5.1:56642839-56642858 MS.gene67274:CDS 40.0%
GCATCCAATCAAGAGTTAGT+GGG + chr5.1:56644719-56644738 MS.gene67274:CDS 40.0%
GTGGTCCCATAATAGATTTG+AGG - chr5.1:56643125-56643144 None:intergenic 40.0%
TCAACCCAAAAACGCTGAAA+TGG + chr5.1:56644344-56644363 MS.gene67274:intron 40.0%
TTAACGATTTCGGTCTAGTG+CGG - chr5.1:56643196-56643215 None:intergenic 40.0%
TTAGTCGTTCTCTTTCTTGC+CGG - chr5.1:56642657-56642676 None:intergenic 40.0%
TTATTGCCATGAACATGCAC+TGG + chr5.1:56644585-56644604 MS.gene67274:CDS 40.0%
! GCACTGGAGTATCTTCTTAA+GGG + chr5.1:56644601-56644620 MS.gene67274:CDS 40.0%
! TGCACTGGAGTATCTTCTTA+AGG + chr5.1:56644600-56644619 MS.gene67274:CDS 40.0%
! TTTTCAGCAGGTGTTTCCTT+TGG - chr5.1:56642858-56642877 None:intergenic 40.0%
AGGGAGTGAAACTTGCATCT+CGG + chr5.1:56644620-56644639 MS.gene67274:CDS 45.0%
CTCACAAGAGATTACAAGGC+TGG + chr5.1:56644679-56644698 MS.gene67274:CDS 45.0%
GAAGCTACGGGAATAGAAAC+GGG - chr5.1:56644450-56644469 None:intergenic 45.0%
GAGTTAGTGGGATGCTTCAA+AGG + chr5.1:56644731-56644750 MS.gene67274:CDS 45.0%
GATACTCCAGTGCATGTTCA+TGG - chr5.1:56644594-56644613 None:intergenic 45.0%
GCATCCCACTAACTCTTGAT+TGG - chr5.1:56644726-56644745 None:intergenic 45.0%
GGCAAGAAAGAGAACGACTA+AGG + chr5.1:56642656-56642675 MS.gene67274:CDS 45.0%
GGCATCCAATCAAGAGTTAG+TGG + chr5.1:56644718-56644737 MS.gene67274:CDS 45.0%
TGGAAAGAGTTCAAACCAGC+TGG - chr5.1:56644799-56644818 None:intergenic 45.0%
TTGCAAAGCAGAATCGAACC+AGG + chr5.1:56643165-56643184 MS.gene67274:intron 45.0%
! AGATGTAGGGAGGAGTATGA+AGG + chr5.1:56642792-56642811 MS.gene67274:CDS 45.0%
!! GCTGGTTTGAACTCTTTCCA+TGG + chr5.1:56644799-56644818 MS.gene67274:CDS 45.0%
!!! AAAATATAATTTTTTTTTTG+AGG + chr5.1:56643957-56643976 MS.gene67274:intron 5.0%
!!! TTTTAAAATCTATAATTTAA+TGG - chr5.1:56643507-56643526 None:intergenic 5.0%
!!! TTTTATTTATAGTATAAATA+AGG + chr5.1:56644376-56644395 MS.gene67274:intron 5.0%
CGATGTGCACGCTAGATGTA+GGG + chr5.1:56642779-56642798 MS.gene67274:CDS 50.0%
GGGAATAGAAACGGGAGAGA+AGG - chr5.1:56644442-56644461 None:intergenic 50.0%
TCAAACCAGCTGGCATGATG+AGG - chr5.1:56644789-56644808 None:intergenic 50.0%
TCAGCAGGTGTTTCCTTTGG+TGG - chr5.1:56642855-56642874 None:intergenic 50.0%
TCGATGTGCACGCTAGATGT+AGG + chr5.1:56642778-56642797 MS.gene67274:CDS 50.0%
! GCTGACAACCACCTTTTTGC+GGG - chr5.1:56642684-56642703 None:intergenic 50.0%
! GGCTGACAACCACCTTTTTG+CGG - chr5.1:56642685-56642704 None:intergenic 50.0%
!! AAGAGTGCTTCTCGCTGGAT+CGG - chr5.1:56642756-56642775 None:intergenic 50.0%
!! GGGATGCTTCAAAGGCATCA+GGG + chr5.1:56644739-56644758 MS.gene67274:CDS 50.0%
!! TGGGATGCTTCAAAGGCATC+AGG + chr5.1:56644738-56644757 MS.gene67274:CDS 50.0%
!!! GCACGCTTGTCATTTTCAGC+AGG - chr5.1:56642870-56642889 None:intergenic 50.0%
ACCAGCTGGCATGATGAGGA+AGG - chr5.1:56644785-56644804 None:intergenic 55.0%
ACCTTCCTCATCATGCCAGC+TGG + chr5.1:56644781-56644800 MS.gene67274:CDS 55.0%
TGCTTCTCGCTGGATCGGTT+CGG - chr5.1:56642751-56642770 None:intergenic 55.0%
TGTGCACGCTAGATGTAGGG+AGG + chr5.1:56642782-56642801 MS.gene67274:CDS 55.0%
! CGTTCTCTTTCTTGCCGGCT+TGG - chr5.1:56642652-56642671 None:intergenic 55.0%
! GGGAGGAGTATGAAGGCAAG+AGG + chr5.1:56642799-56642818 MS.gene67274:CDS 55.0%
!! CGATCAAGAGTGCTTCTCGC+TGG - chr5.1:56642761-56642780 None:intergenic 55.0%
CGGTCTAGTGCGGTTTCACC+TGG - chr5.1:56643186-56643205 None:intergenic 60.0%
! CGACTAAGGCGCCCGCAAAA+AGG + chr5.1:56642670-56642689 MS.gene67274:CDS 60.0%
! CTAAGGCGCCCGCAAAAAGG+TGG + chr5.1:56642673-56642692 MS.gene67274:CDS 60.0%
! GCTTCTCGCTGGATCGGTTC+GGG - chr5.1:56642750-56642769 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 56642636 56644828 56642636 ID=MS.gene67274
chr5.1 mRNA 56642636 56644828 56642636 ID=MS.gene67274.t1;Parent=MS.gene67274
chr5.1 exon 56642636 56642905 56642636 ID=MS.gene67274.t1.exon1;Parent=MS.gene67274.t1
chr5.1 CDS 56642636 56642905 56642636 ID=cds.MS.gene67274.t1;Parent=MS.gene67274.t1
chr5.1 exon 56644568 56644828 56644568 ID=MS.gene67274.t1.exon2;Parent=MS.gene67274.t1
chr5.1 CDS 56644568 56644828 56644568 ID=cds.MS.gene67274.t1;Parent=MS.gene67274.t1
Gene Sequence

>MS.gene67274

ATGAGGCATGGAGCCAAGCCGGCAAGAAAGAGAACGACTAAGGCGCCCGCAAAAAGGTGGTTGTCAGCCGAACAACATACTACTCCTGTTGAAGAAGAATGTCATTTCGATCCCGAACCGATCCAGCGAGAAGCACTCTTGATCGATGTGCACGCTAGATGTAGGGAGGAGTATGAAGGCAAGAGGAGATCAAAATGGAAAAGGAGTGTTAATGAACCACCAAAGGAAACACCTGCTGAAAATGACAAGCGTGCTCAACAAGAAATGCACGTATAATAACATACTACTTTATTGTGCCTTTGAATATATATCTTCAATGTGTGACATGATCTGATATGGTGTGAATATATCTTTATGTGTCACTCAAAGATCTTTTTAATCTCATCATCTGTAGTAATAAGTACGGTTACAACTACTGTTAAGTTTTTTAATTTTTTGTTTTTACGTGAAACGGGAATCCTTTATGTTCAACTTCCTAGAATAATCCCTCAAATCTATTATGGGACCACAATGATTGACAAAACTCTCTTTGCAAAGCAGAATCGAACCAGGTGAAACCGCACTAGACCGAAATCGTTAAACCCAAAACAAATCAAGGAGGAATATGGTATGGTCGATTTTTGATTTATTGTTCATCCTTTTTTTTCATCGCCAGAACGTTTTTTTATTTTATTTTTTCCAGTAAATTGAGTTTAATCTTTTTCAAGAAGTTTGTTGTTGATTTTTATAATAATATATATAATTGCTCCTCTTATTGTAAAATCGTTCCTATTTCTACTATAATTAAAAAAGATTGTTTTCGACTTTTTTTGAAGGAAACGTTTGTTTTCGATTATTGATAACTAAATATCACAAATGATCATGTAATCCATTAAATTATAGATTTTAAAAGATTGTCGGAAGGCAAATTAAGTAAGTTTTAATCAAAAAAGATTAATTTGTTTAATAGATTAAGGATTAATTTAGTAGTATGTAACCAAAAAAAAATTTAGTAGTAGTTTTTTTTTTGAAGGAAAATTTAGTAGTAGTTTTTTTTTTGACAAGTAGTAGTAGTTGTATGGTAGTAGATTAATTTGTTTAATTAAATCATAATTGCTTTATCCGAATTTCACTCCTTCAAATCCAAATCTCAAACTTATCACTTTGTTTTTTCTTCTAGAAAAAAGATACGAAACAAAATTAGTTTAGATTACACTTAATTATATTTAACTTTACTATTATATAAGTAGCAAAAAGATGGGTTTTTCGCTATTTTTATTGCGCTAACGTTTGAGAATTATGAACGATGTTTTTTATGAAATTTTAATGTTGATTAAAAGTAAAAATATAATTTTTTTTTTGAGGAAAAAAGTAAAAATATAAATGTTTTTTGCTTTCAAATTATAACAAATCAAACTAATCATGATTATCTCTTTCAATGAAATAAAAAATATAACAAAATAATATAATCTATATTCTTATTTTTTTAATGACACCAACAACAATATTTATTATAGAAAGATCATTGTTGTTTAAGAGTATAATTGGGATAATGAAAAAGTAAGTATTGATACACTCATATATTTTGTTACACTTTTTAAATGATAAAGGAAAAAACTATACATTTGTGTTTGTTACATTTTTATTTTAATATTTAAATTTATTCTTATCTATTTGTTATATGTGTTATTTGTATTGAAAGTTGAGGGTATTTTTGGAAGAAAAAAATTCAACCCAAAAACGCTGAAATGGATAGTTTTCTTTTATTTATAGTATAAATAAGGTACTCCCCTAAAAAAATAACGTACTCTGAAATAGGGTTAACCTTCTCTCCCGTTTCTATTCCCGTAGCTTCTGAATCCTCTTTCACTAGAATCGTTAGTTTTTTTTGGTTTTATTATTTTGAATTGGAATTTGTAGTCTTACTGGATATTACTATTCTGAATAATAAAGATGAACATCCCTCGAAATTATTGCCATGAACATGCACTGGAGTATCTTCTTAAGGGAGTGAAACTTGCATCTCGGTACGATAACAGCATAAAGTACGATGAGTTTTTAAAACTCACAAGAGATTACAAGGCTGGAAGAATTGATATAATAGGCATCCAATCAAGAGTTAGTGGGATGCTTCAAAGGCATCAGGGTTTATTATTCGGATTCAACACCTTCCTCATCATGCCAGCTGGTTTGAACTCTTTCCATGGTACGTAA
Protein sequence

>MS.gene67274.t1

MRHGAKPARKRTTKAPAKRWLSAEQHTTPVEEECHFDPEPIQREALLIDVHARCREEYEGKRRSKWKRSVNEPPKETPAENDKRAQQEMHMNIPRNYCHEHALEYLLKGVKLASRYDNSIKYDEFLKLTRDYKAGRIDIIGIQSRVSGMLQRHQGLLFGFNTFLIMPAGLNSFHGT