AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67437

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67437.t1 MTR_3g071590 99.371 159 1 0 1 159 1 159 7.23e-118 329
MS.gene67437.t1 MTR_5g083230 75.155 161 31 4 1 156 1 157 4.86e-81 237
MS.gene67437.t1 MTR_3g031660 65.359 153 40 2 3 142 2 154 3.95e-69 207
MS.gene67437.t1 MTR_2g068760 75.455 110 27 0 4 113 9 118 8.49e-58 177
MS.gene67437.t1 MTR_4g083680 70.339 118 31 1 2 115 5 122 1.92e-56 175
MS.gene67437.t1 MTR_2g093310 63.063 111 41 0 7 117 32 142 1.05e-50 161
MS.gene67437.t1 MTR_4g060950 65.179 112 38 1 7 117 23 134 6.83e-50 159
MS.gene67437.t1 MTR_1g095850 71.287 101 29 0 9 109 73 173 1.11e-48 157
MS.gene67437.t1 MTR_5g083960 52.795 161 67 3 4 158 35 192 2.01e-48 155
MS.gene67437.t1 MTR_3g094690 63.393 112 41 0 4 115 43 154 3.79e-47 153
MS.gene67437.t1 MTR_6g005080 52.113 142 59 3 3 144 5 137 1.44e-46 149
MS.gene67437.t1 MTR_7g033800 59.829 117 43 1 5 117 8 124 2.41e-46 149
MS.gene67437.t1 MTR_6g005070 53.571 140 56 3 5 144 6 136 3.08e-46 148
MS.gene67437.t1 MTR_3g077240 66.346 104 35 0 6 109 8 111 5.43e-46 149
MS.gene67437.t1 MTR_4g105170 66.019 103 35 0 7 109 10 112 7.54e-46 148
MS.gene67437.t1 MTR_3g073690 61.111 108 42 0 1 108 2 109 1.61e-45 148
MS.gene67437.t1 MTR_3g094690 62.500 112 41 1 4 115 43 153 1.42e-44 146
MS.gene67437.t1 MTR_5g075020 66.038 106 36 0 4 109 39 144 1.55e-42 141
MS.gene67437.t1 MTR_3g071420 63.366 101 37 0 5 105 11 111 1.71e-42 139
MS.gene67437.t1 MTR_5g075020 66.038 106 36 0 4 109 43 148 2.10e-42 141
MS.gene67437.t1 MTR_3g452660 59.615 104 42 0 5 108 4 107 2.43e-42 142
MS.gene67437.t1 MTR_5g017950 58.333 108 45 0 1 108 1 108 7.86e-42 142
MS.gene67437.t1 MTR_5g083010 62.376 101 38 0 5 105 8 108 2.03e-41 137
MS.gene67437.t1 MTR_7g096530 57.143 105 44 1 5 108 11 115 1.93e-37 128
MS.gene67437.t1 MTR_8g040900 59.615 104 42 0 5 108 4 107 5.65e-37 127
MS.gene67437.t1 MTR_1g070220 51.695 118 56 1 8 124 15 132 1.08e-36 125
MS.gene67437.t1 MTR_5g093010 48.624 109 56 0 9 117 6 114 4.03e-36 122
MS.gene67437.t1 MTR_7g074990 56.863 102 43 1 8 108 20 121 5.88e-36 124
MS.gene67437.t1 MTR_7g028905 52.941 102 48 0 7 108 4 105 1.24e-35 120
MS.gene67437.t1 MTR_7g096610 43.919 148 80 2 1 145 1 148 3.47e-35 122
MS.gene67437.t1 MTR_8g036105 56.075 107 46 1 7 112 17 123 7.06e-35 121
MS.gene67437.t1 MTR_7g075230 48.092 131 65 2 2 129 6 136 7.19e-35 120
MS.gene67437.t1 MTR_8g036085 41.290 155 78 3 7 148 22 176 2.02e-34 120
MS.gene67437.t1 MTR_1g070205 45.139 144 70 5 8 146 12 151 3.49e-34 120
MS.gene67437.t1 MTR_6g027710 45.299 117 63 1 7 122 39 155 1.14e-33 118
MS.gene67437.t1 MTR_5g080470 54.082 98 44 1 7 103 6 103 2.31e-33 116
MS.gene67437.t1 MTR_8g079660 47.525 101 53 0 8 108 8 108 1.26e-29 108
MS.gene67437.t1 MTR_8g079620 44.554 101 56 0 8 108 8 108 1.49e-28 105
MS.gene67437.t1 MTR_6g027700 46.535 101 53 1 8 107 7 107 2.46e-28 104
MS.gene67437.t1 MTR_8g079580 44.545 110 60 1 1 109 1 110 1.19e-27 103
MS.gene67437.t1 MTR_4g107450 38.776 147 81 3 1 140 3 147 5.83e-27 102
MS.gene67437.t1 MTR_6g011230 40.404 99 59 0 3 101 6 104 6.07e-26 98.2
MS.gene67437.t1 MTR_6g011250 38.971 136 77 3 9 143 10 140 6.39e-26 97.4
MS.gene67437.t1 MTR_2g100020 38.462 104 64 0 5 108 16 119 1.63e-22 90.9
MS.gene67437.t1 MTR_8g017090 37.500 104 65 0 5 108 9 112 5.13e-22 89.7
MS.gene67437.t1 MTR_6g011200 43.750 80 44 1 9 87 10 89 5.15e-22 89.0
MS.gene67437.t1 MTR_4g088035 38.202 89 55 0 7 95 5 93 2.75e-19 82.0
MS.gene67437.t1 MTR_5g085390 33.333 147 89 4 9 146 10 156 5.03e-19 80.9
MS.gene67437.t1 MTR_4g099230 39.362 94 52 2 9 97 3 96 1.27e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67437.t1 AT2G30130 77.698 139 25 2 1 135 1 137 4.95e-73 218
MS.gene67437.t1 AT1G16530 67.544 114 36 1 3 115 9 122 2.59e-52 164
MS.gene67437.t1 AT1G07900 64.865 111 39 0 7 117 32 142 1.57e-50 160
MS.gene67437.t1 AT2G28500 65.138 109 38 0 7 115 54 162 6.40e-50 160
MS.gene67437.t1 AT1G31320 71.171 111 32 0 4 114 9 119 9.28e-50 157
MS.gene67437.t1 AT2G30340 64.151 106 38 0 4 109 48 153 2.25e-46 152
MS.gene67437.t1 AT2G30340 64.151 106 38 0 4 109 49 154 2.49e-46 152
MS.gene67437.t1 AT3G27650 62.385 109 41 0 1 109 32 140 3.89e-46 148
MS.gene67437.t1 AT5G63090 66.990 103 34 0 6 108 9 111 5.67e-46 149
MS.gene67437.t1 AT5G63090 66.990 103 34 0 6 108 9 111 5.67e-46 149
MS.gene67437.t1 AT5G63090 66.990 103 34 0 6 108 9 111 5.67e-46 149
MS.gene67437.t1 AT5G63090 66.990 103 34 0 6 108 9 111 5.67e-46 149
MS.gene67437.t1 AT2G40470 59.542 131 51 1 4 132 16 146 1.72e-45 148
MS.gene67437.t1 AT2G40470 59.542 131 51 1 4 132 41 171 3.49e-45 148
MS.gene67437.t1 AT3G26660 62.264 106 40 0 9 114 6 111 4.22e-45 144
MS.gene67437.t1 AT3G26620 62.264 106 40 0 9 114 6 111 5.37e-45 144
MS.gene67437.t1 AT2G23660 56.731 104 45 0 6 109 3 106 1.91e-42 143
MS.gene67437.t1 AT2G23660 56.731 104 45 0 6 109 3 106 1.91e-42 143
MS.gene67437.t1 AT2G23660 56.731 104 45 0 6 109 3 106 1.91e-42 143
MS.gene67437.t1 AT3G11090 56.000 125 53 1 5 127 8 132 2.95e-42 138
MS.gene67437.t1 AT5G66870 52.000 125 60 0 4 128 3 127 1.62e-41 141
MS.gene67437.t1 AT1G65620 61.905 105 40 0 4 108 5 109 5.70e-39 131
MS.gene67437.t1 AT1G65620 61.905 105 40 0 4 108 5 109 5.70e-39 131
MS.gene67437.t1 AT1G65620 61.905 105 40 0 4 108 5 109 5.70e-39 131
MS.gene67437.t1 AT1G65620 61.905 105 40 0 4 108 5 109 5.70e-39 131
MS.gene67437.t1 AT1G65620 61.905 105 40 0 4 108 5 109 5.70e-39 131
MS.gene67437.t1 AT4G00210 54.310 116 49 2 1 112 1 116 2.25e-37 127
MS.gene67437.t1 AT3G03760 55.769 104 45 1 7 109 50 153 7.75e-37 128
MS.gene67437.t1 AT2G42430 57.547 106 44 1 4 108 11 116 8.91e-37 127
MS.gene67437.t1 AT2G45410 57.282 103 43 1 11 112 19 121 2.11e-34 119
MS.gene67437.t1 AT2G42440 46.269 134 66 3 7 139 6 134 3.23e-34 120
MS.gene67437.t1 AT3G58190 54.639 97 43 1 8 103 11 107 3.54e-33 117
MS.gene67437.t1 AT2G31310 50.926 108 51 2 1 107 1 107 1.64e-32 114
MS.gene67437.t1 AT2G45420 51.923 104 49 1 11 113 40 143 1.73e-32 116
MS.gene67437.t1 AT4G00220 52.885 104 48 1 11 113 20 123 4.21e-32 114
MS.gene67437.t1 AT3G50510 43.902 123 67 1 6 126 10 132 2.36e-30 109
MS.gene67437.t1 AT3G50510 43.902 123 67 1 6 126 10 132 2.36e-30 109
MS.gene67437.t1 AT5G06080 51.376 109 47 2 7 114 6 109 7.38e-30 107
MS.gene67437.t1 AT5G35900 36.429 140 71 2 9 148 6 127 6.27e-26 98.2
MS.gene67437.t1 AT1G72980 38.525 122 74 1 3 123 8 129 4.93e-25 95.9
MS.gene67437.t1 AT3G47870 37.037 108 68 0 1 108 29 136 2.63e-24 96.3
MS.gene67437.t1 AT3G13850 35.537 121 74 1 9 125 37 157 2.42e-23 92.8
MS.gene67437.t1 AT1G06280 49.351 77 39 0 9 85 25 101 8.38e-21 84.7
MS.gene67437.t1 AT4G22700 35.821 134 72 4 9 141 6 126 3.45e-18 77.8
MS.gene67437.t1 AT2G19820 38.542 96 58 1 7 101 11 106 7.45e-17 72.4
MS.gene67437.t1 AT5G15060 40.000 80 44 2 3 79 12 90 4.95e-14 65.5
MS.gene67437.t1 AT1G36000 29.885 87 61 0 8 94 9 95 1.80e-12 61.2
MS.gene67437.t1 AT3G27940 36.364 66 42 0 7 72 4 69 3.06e-11 58.5
MS.gene67437.t1 AT3G27940 36.364 66 42 0 7 72 4 69 3.06e-11 58.5
MS.gene67437.t1 AT3G27940 36.364 66 42 0 7 72 4 69 3.06e-11 58.5

Find 20 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAGCACAACGGCGTCTA+AGG 0.285930 3.1:-58564001 None:intergenic
GCATCAGCTCTTTGTTGAAT+AGG 0.314081 3.1:-58564488 None:intergenic
GGATCATGTGAAGGGAAATA+AGG 0.350330 3.1:-58564038 None:intergenic
AGAGTGAAAGACCCTGTTTA+TGG 0.387679 3.1:+58564542 MS.gene67437:CDS
GTTGATCATCATGTTTCATC+TGG 0.388561 3.1:-58564652 None:intergenic
GCTCCAACACAACCATAAAC+AGG 0.400328 3.1:-58564554 None:intergenic
GCATTTGAAGCTCAGAAACT+TGG 0.418459 3.1:-58564595 None:intergenic
TGAATTACATTGCTTGAAGA+AGG 0.450118 3.1:-58564743 None:intergenic
GACCCTGTTTATGGTTGTGT+TGG 0.486001 3.1:+58564551 MS.gene67437:CDS
GATCATAAGATTTATGATCA+AGG 0.495019 3.1:-58564700 None:intergenic
CCTTAGACGCCGTTGTGCTA+AGG 0.517412 3.1:+58564001 MS.gene67437:CDS
GAAGGAAAGTTGAAATACTG+AGG 0.552181 3.1:-58564725 None:intergenic
ATCATAAGATTTATGATCAA+GGG 0.554510 3.1:-58564699 None:intergenic
CAACGTTAGCAAAATGCTAC+AGG 0.557355 3.1:+58564100 MS.gene67437:CDS
AAAATTTGCAATAGTTCACA+AGG 0.586403 3.1:+58564064 MS.gene67437:CDS
AGGAGTTTGCATGAAGCACA+AGG 0.592427 3.1:-58563981 None:intergenic
GAGTTTGCATGAAGCACAAG+GGG 0.629954 3.1:-58563979 None:intergenic
CTCCAACACAACCATAAACA+GGG 0.640494 3.1:-58564553 None:intergenic
GGAGTTTGCATGAAGCACAA+GGG 0.671143 3.1:-58563980 None:intergenic
AGATACAATCCTTAGCACAA+CGG 0.718268 3.1:-58564010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTCAAAATCGAATTT+TGG - chr3.1:58564344-58564363 None:intergenic 15.0%
!! ATCATAAGATTTATGATCAA+GGG - chr3.1:58564702-58564721 None:intergenic 20.0%
!! ATGTTACATCGTTTAAAAAT+TGG + chr3.1:58564282-58564301 MS.gene67437:intron 20.0%
!!! AAAAGTGCAATTTTCTTATT+AGG + chr3.1:58564400-58564419 MS.gene67437:intron 20.0%
!!! AAGTTTTTCTTTTTCTTTCA+AGG - chr3.1:58564151-58564170 None:intergenic 20.0%
!!! TGAACTATTGCAAATTTTTT+AGG - chr3.1:58564062-58564081 None:intergenic 20.0%
! AAAATTTGCAATAGTTCACA+AGG + chr3.1:58564064-58564083 MS.gene67437:CDS 25.0%
! AACGATGTAACATTAAAATG+TGG - chr3.1:58564275-58564294 None:intergenic 25.0%
! ACGATGTAACATTAAAATGT+GGG - chr3.1:58564274-58564293 None:intergenic 25.0%
! GACTTATTGTACAATGTTAT+AGG + chr3.1:58564460-58564479 MS.gene67437:intron 25.0%
! GATCATAAGATTTATGATCA+AGG - chr3.1:58564703-58564722 None:intergenic 25.0%
!! AGAACAAAAGTACAATTAAG+CGG - chr3.1:58564223-58564242 None:intergenic 25.0%
!! ATTTCTTGATTTTGCATTAG+AGG - chr3.1:58564677-58564696 None:intergenic 25.0%
!!! AATTTTTTAGGATCATGTGA+AGG - chr3.1:58564050-58564069 None:intergenic 25.0%
!!! ATTTTTTAGGATCATGTGAA+GGG - chr3.1:58564049-58564068 None:intergenic 25.0%
CGGATGTTTGTTTCAAAAAA+TGG - chr3.1:58564313-58564332 None:intergenic 30.0%
TGAATTACATTGCTTGAAGA+AGG - chr3.1:58564746-58564765 None:intergenic 30.0%
! AATAACCATTTTCAGCCAAA+AGG - chr3.1:58564440-58564459 None:intergenic 30.0%
! ATAACCATTTTCAGCCAAAA+GGG - chr3.1:58564439-58564458 None:intergenic 30.0%
!!! GTATAAGAGAGCGTTATTTT+AGG + chr3.1:58564179-58564198 MS.gene67437:intron 30.0%
AGATACAATCCTTAGCACAA+CGG - chr3.1:58564013-58564032 None:intergenic 35.0%
GAAGGAAAGTTGAAATACTG+AGG - chr3.1:58564728-58564747 None:intergenic 35.0%
GTTGATCATCATGTTTCATC+TGG - chr3.1:58564655-58564674 None:intergenic 35.0%
! GCAATAGTTCACAAGGTTTT+TGG + chr3.1:58564071-58564090 MS.gene67437:CDS 35.0%
!!! GTTTTGAGTTTTGACCCTTT+TGG + chr3.1:58564422-58564441 MS.gene67437:intron 35.0%
AGAGTGAAAGACCCTGTTTA+TGG + chr3.1:58564542-58564561 MS.gene67437:CDS 40.0%
CAACGTTAGCAAAATGCTAC+AGG + chr3.1:58564100-58564119 MS.gene67437:CDS 40.0%
CTCCAACACAACCATAAACA+GGG - chr3.1:58564556-58564575 None:intergenic 40.0%
GCATCAGCTCTTTGTTGAAT+AGG - chr3.1:58564491-58564510 None:intergenic 40.0%
GGATCATGTGAAGGGAAATA+AGG - chr3.1:58564041-58564060 None:intergenic 40.0%
! GCATTTGAAGCTCAGAAACT+TGG - chr3.1:58564598-58564617 None:intergenic 40.0%
! TAGCATTTTGCTAACGTTGC+TGG - chr3.1:58564099-58564118 None:intergenic 40.0%
!! ATCGAATTTTGGTCGAAACG+CGG - chr3.1:58564333-58564352 None:intergenic 40.0%
!! TTGACCCTTTTGGCTGAAAA+TGG + chr3.1:58564432-58564451 MS.gene67437:intron 40.0%
!!! GCTCAGAAACTTGGTTTTGA+AGG - chr3.1:58564589-58564608 None:intergenic 40.0%
AGGAGTTTGCATGAAGCACA+AGG - chr3.1:58563984-58564003 None:intergenic 45.0%
GACCCTGTTTATGGTTGTGT+TGG + chr3.1:58564551-58564570 MS.gene67437:CDS 45.0%
GAGTTTGCATGAAGCACAAG+GGG - chr3.1:58563982-58564001 None:intergenic 45.0%
GCTCCAACACAACCATAAAC+AGG - chr3.1:58564557-58564576 None:intergenic 45.0%
GGAGTTTGCATGAAGCACAA+GGG - chr3.1:58563983-58564002 None:intergenic 45.0%
! TCAAGGAAGCATAGAAAGCG+TGG - chr3.1:58564134-58564153 None:intergenic 45.0%
CCTTAGACGCCGTTGTGCTA+AGG + chr3.1:58564001-58564020 MS.gene67437:CDS 55.0%
CCTTAGCACAACGGCGTCTA+AGG - chr3.1:58564004-58564023 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 58563960 58564799 58563960 ID=MS.gene67437
chr3.1 mRNA 58563960 58564799 58563960 ID=MS.gene67437.t1;Parent=MS.gene67437
chr3.1 exon 58563960 58564121 58563960 ID=MS.gene67437.t1.exon1;Parent=MS.gene67437.t1
chr3.1 CDS 58563960 58564121 58563960 ID=cds.MS.gene67437.t1;Parent=MS.gene67437.t1
chr3.1 exon 58564482 58564799 58564482 ID=MS.gene67437.t1.exon2;Parent=MS.gene67437.t1
chr3.1 CDS 58564482 58564799 58564482 ID=cds.MS.gene67437.t1;Parent=MS.gene67437.t1
Gene Sequence

>MS.gene67437

ATGGGTGGTAATACTACTTCCCCTTGTGCTTCATGCAAACTCCTTAGACGCCGTTGTGCTAAGGATTGTATCTTTGCTCCTTATTTCCCTTCACATGATCCTAAAAAATTTGCAATAGTTCACAAGGTTTTTGGTGCCAGCAACGTTAGCAAAATGCTACAGGTACTTCATCCACGCTTTCTATGCTTCCTTGAAAGAAAAAGAAAAACTTCATAGTGTGTATAAGAGAGCGTTATTTTAGGTTTAGTTTTATTATCTTTCCGCTTAATTGTACTTTTGTTCTTCTTATTTTATCATGTTCACAAAAATAGCCCACATTTTAATGTTACATCGTTTAAAAATTGGTCACACCATTTTTTGAAACAAACATCCGCGTTTCGACCAAAATTCGATTTTGAAAAATATGACTATTTTTGTGAACGTGATAAAATAGAAAGATCAAAAGTGCAATTTTCTTATTAGGTTTTGAGTTTTGACCCTTTTGGCTGAAAATGGTTATTGACTTATTGTACAATGTTATAGGAACTTCCTATTCAACAAAGAGCTGATGCAGTAAGCAGTTTAGTATATGAAGCAAATGCAAGAGTGAAAGACCCTGTTTATGGTTGTGTTGGAGCAATATCATACCTTCAAAACCAAGTTTCTGAGCTTCAAATGCAGCTTGCAGTAGCACAAGCAGAAATACTTTGCATCCAGATGAAACATGATGATCAACCTCTAATGCAAAATCAAGAAATAGCCCTTGATCATAAATCTTATGATCTTCCTCAGTATTTCAACTTTCCTTCTTCAAGCAATGTAATTCATCATGATTCTTTCTCAAGAGAGAGAATTTTTTGA
Protein sequence

>MS.gene67437.t1

MGGNTTSPCASCKLLRRRCAKDCIFAPYFPSHDPKKFAIVHKVFGASNVSKMLQELPIQQRADAVSSLVYEANARVKDPVYGCVGAISYLQNQVSELQMQLAVAQAEILCIQMKHDDQPLMQNQEIALDHKSYDLPQYFNFPSSSNVIHHDSFSRERIF