Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67559.t1 | AES95288.1 | 51.6 | 184 | 46 | 5 | 1 | 158 | 1 | 167 | 1.10E-32 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67559.t1 | G7K021 | 51.6 | 184 | 46 | 5 | 1 | 158 | 1 | 167 | 8.3e-33 | 149.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene67559.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67559.t1 | MTR_5g023880 | 57.609 | 184 | 35 | 5 | 1 | 158 | 1 | 167 | 3.30e-58 | 180 |
| MS.gene67559.t1 | MTR_5g023900 | 54.802 | 177 | 58 | 3 | 1 | 157 | 1 | 175 | 1.62e-57 | 179 |
| MS.gene67559.t1 | MTR_5g023870 | 67.164 | 134 | 25 | 4 | 1 | 130 | 1 | 119 | 3.12e-53 | 165 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 68 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCGGAAGGGTTAGGTTT+AGG | 0.237072 | 5.4:-15327578 | None:intergenic |
| TCTCGGAAGGGTTAGGTTTA+GGG | 0.267292 | 5.4:-15327577 | None:intergenic |
| TTGAGGGGACTTGTTGTTGG+TGG | 0.289994 | 5.4:-15327609 | None:intergenic |
| GGGCTTCTTGATCGTCCTCT+TGG | 0.324765 | 5.4:-15328512 | None:intergenic |
| TGCTTATCAGTTGTATCGGC+TGG | 0.335441 | 5.4:-15328533 | None:intergenic |
| GTCTTGAGGGGACTTGTTGT+TGG | 0.343036 | 5.4:-15327612 | None:intergenic |
| AGTTGGGTTGGTTTCTTTGA+GGG | 0.364234 | 5.4:-15327654 | None:intergenic |
| GTTGAATTCTCGGAAGGGTT+AGG | 0.378551 | 5.4:-15327584 | None:intergenic |
| GGCTTCTTGATCGTCCTCTT+GGG | 0.380085 | 5.4:-15328511 | None:intergenic |
| TAGTTGGGTTGGTTTCTTTG+AGG | 0.387471 | 5.4:-15327655 | None:intergenic |
| AAAGAACTCTGAAGGGTAGT+TGG | 0.402173 | 5.4:-15327671 | None:intergenic |
| TGAGGGGACTTGTTGTTGGT+GGG | 0.402381 | 5.4:-15327608 | None:intergenic |
| AGGGGACTTGTTGTTGGTGG+GGG | 0.408399 | 5.4:-15327606 | None:intergenic |
| TCAGAGTCCTCTATCTCTAT+TGG | 0.410192 | 5.4:-15328418 | None:intergenic |
| CTCTTGGGCCTTCCTTCCTC+TGG | 0.410975 | 5.4:-15328496 | None:intergenic |
| AAGAACTCTGAAGGGTAGTT+GGG | 0.428150 | 5.4:-15327670 | None:intergenic |
| CATGGTTATTCAGCACCTCC+TGG | 0.431962 | 5.4:+15327863 | MS.gene67559:CDS |
| TACAATGTCAAGTCAGGAAG+AGG | 0.434728 | 5.4:+15328441 | MS.gene67559:CDS |
| TCTTTAAGAGCTCGTGAGAA+TGG | 0.434908 | 5.4:+15327689 | MS.gene67559:CDS |
| CAGTGTTGTTGTTTGCGCCT+TGG | 0.435389 | 5.4:-15328471 | None:intergenic |
| ACCTCAACCTGATCAATATG+AGG | 0.437745 | 5.4:+15327922 | MS.gene67559:CDS |
| AGGTGGGGTGGCTGTTGATG+TGG | 0.445640 | 5.4:-15327903 | None:intergenic |
| CATCAGAGGGAGGGTCTTGA+GGG | 0.445658 | 5.4:-15327625 | None:intergenic |
| CCCTCATATTGATCAGGTTG+AGG | 0.453934 | 5.4:-15327923 | None:intergenic |
| GCAAACATGTGTTTCCCACT+TGG | 0.463748 | 5.4:+15327792 | MS.gene67559:CDS |
| CTTTAAGAGCTCGTGAGAAT+GGG | 0.465502 | 5.4:+15327690 | MS.gene67559:CDS |
| AAGTGATGGTCAAGCTATGA+AGG | 0.473835 | 5.4:+15327739 | MS.gene67559:CDS |
| AAGCCAGCCAATAGAGATAG+AGG | 0.474391 | 5.4:+15328411 | MS.gene67559:CDS |
| CTCTGATACAATGTCAAGTC+AGG | 0.478904 | 5.4:+15328435 | MS.gene67559:CDS |
| CATATTGATCAGGTTGAGGT+GGG | 0.500892 | 5.4:-15327919 | None:intergenic |
| AGTCCTCTATCTCTATTGGC+TGG | 0.503791 | 5.4:-15328414 | None:intergenic |
| TCATCAGAGGGAGGGTCTTG+AGG | 0.508193 | 5.4:-15327626 | None:intergenic |
| ACTCTGAAGGGTAGTTGGGT+TGG | 0.513668 | 5.4:-15327666 | None:intergenic |
| AGCTCGTGAGAATGGGAAGC+TGG | 0.517710 | 5.4:+15327697 | MS.gene67559:CDS |
| CAAACATGTGTTTCCCACTT+GGG | 0.531651 | 5.4:+15327793 | MS.gene67559:CDS |
| GCTTATCAGTTGTATCGGCT+GGG | 0.531837 | 5.4:-15328532 | None:intergenic |
| TATATTTGTTGTCATCCTCA+TGG | 0.532111 | 5.4:+15327845 | MS.gene67559:CDS |
| TTGATCAGGTTGAGGTGGGG+TGG | 0.544768 | 5.4:-15327915 | None:intergenic |
| GAGGGGACTTGTTGTTGGTG+GGG | 0.544991 | 5.4:-15327607 | None:intergenic |
| TCATATTGATCAGGTTGAGG+TGG | 0.558741 | 5.4:-15327920 | None:intergenic |
| ACACTGGACCAGAGGAAGGA+AGG | 0.561168 | 5.4:+15328488 | MS.gene67559:CDS |
| TTTGAGGGATTCATCATCAG+AGG | 0.561409 | 5.4:-15327639 | None:intergenic |
| GAGCTGCTTATCAGTTGTAT+CGG | 0.563493 | 5.4:-15328537 | None:intergenic |
| GAGCTCTTAAAGAACTCTGA+AGG | 0.564348 | 5.4:-15327679 | None:intergenic |
| TTGAGGGATTCATCATCAGA+GGG | 0.579951 | 5.4:-15327638 | None:intergenic |
| CAAGGCGCAAACAACAACAC+TGG | 0.583137 | 5.4:+15328472 | MS.gene67559:CDS |
| AAACAACAACACTGGACCAG+AGG | 0.583233 | 5.4:+15328480 | MS.gene67559:CDS |
| CAGGAAGAGGGAAGGAACCA+AGG | 0.586582 | 5.4:+15328454 | MS.gene67559:CDS |
| GGGGTGGCTGTTGATGTGGA+AGG | 0.586960 | 5.4:-15327899 | None:intergenic |
| ATATTGATCAGGTTGAGGTG+GGG | 0.595131 | 5.4:-15327918 | None:intergenic |
| AACAACACTGGACCAGAGGA+AGG | 0.595528 | 5.4:+15328484 | MS.gene67559:CDS |
| CAGAGGAAGGAAGGCCCAAG+AGG | 0.596615 | 5.4:+15328497 | MS.gene67559:CDS |
| AGTGATGGTCAAGCTATGAA+GGG | 0.603559 | 5.4:+15327740 | MS.gene67559:CDS |
| AATGGGAAGCTGGCAGCTGA+TGG | 0.610197 | 5.4:+15327707 | MS.gene67559:CDS |
| AAGCTGGCAGCTGATGGCGA+AGG | 0.613924 | 5.4:+15327713 | MS.gene67559:CDS |
| AGCTCTTAAAGAACTCTGAA+GGG | 0.626255 | 5.4:-15327678 | None:intergenic |
| AGGGATTCATCATCAGAGGG+AGG | 0.627786 | 5.4:-15327635 | None:intergenic |
| CTTCATCAAAGTAGCCCAAG+TGG | 0.635469 | 5.4:-15327807 | None:intergenic |
| ATGGTCAAGCTATGAAGGGG+AGG | 0.636182 | 5.4:+15327744 | MS.gene67559:CDS |
| ACAATGTCAAGTCAGGAAGA+GGG | 0.642713 | 5.4:+15328442 | MS.gene67559:CDS |
| GGGATTCATCATCAGAGGGA+GGG | 0.650027 | 5.4:-15327634 | None:intergenic |
| CCTCAACCTGATCAATATGA+GGG | 0.650750 | 5.4:+15327923 | MS.gene67559:CDS |
| ATCAGAGGGAGGGTCTTGAG+GGG | 0.650850 | 5.4:-15327624 | None:intergenic |
| TTCATCAAAGTAGCCCAAGT+GGG | 0.657037 | 5.4:-15327806 | None:intergenic |
| TGTCAAGTCAGGAAGAGGGA+AGG | 0.657401 | 5.4:+15328446 | MS.gene67559:CDS |
| GATGGCGAAGGAGTAAGTGA+TGG | 0.658846 | 5.4:+15327725 | MS.gene67559:CDS |
| GTGATGGTCAAGCTATGAAG+GGG | 0.659294 | 5.4:+15327741 | MS.gene67559:CDS |
| GGAGGTGCTGAATAACCATG+AGG | 0.763451 | 5.4:-15327860 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATTAAATAATTGAATCT+TGG | - | chr5.4:15328069-15328088 | None:intergenic | 15.0% |
| !!! | TTTTTCACACTATAATTTTT+AGG | + | chr5.4:15327999-15328018 | MS.gene67559:intron | 15.0% |
| !!! | AATGTTTTTCTTTTGTTACT+AGG | + | chr5.4:15328379-15328398 | MS.gene67559:intron | 20.0% |
| !!! | ACAATAAATAGTTTTGAGAT+AGG | - | chr5.4:15327968-15327987 | None:intergenic | 20.0% |
| ! | CATATATATGTTTGTGTCTA+TGG | - | chr5.4:15328194-15328213 | None:intergenic | 25.0% |
| ! | TTATGAGATCTAATTCATGT+AGG | + | chr5.4:15328237-15328256 | MS.gene67559:intron | 25.0% |
| !!! | TAGTTTTGAGATAGGAAATT+AGG | - | chr5.4:15327960-15327979 | None:intergenic | 25.0% |
| GAATCTTGGACATGATATAT+AGG | - | chr5.4:15328055-15328074 | None:intergenic | 30.0% | |
| TATATAGGCAATAAAACCCT+AGG | - | chr5.4:15328040-15328059 | None:intergenic | 30.0% | |
| !! | TATATTTGTTGTCATCCTCA+TGG | + | chr5.4:15327845-15327864 | MS.gene67559:CDS | 30.0% |
| AGCTCTTAAAGAACTCTGAA+GGG | - | chr5.4:15327681-15327700 | None:intergenic | 35.0% | |
| ! | GTTTTACCCTCATATTGATC+AGG | - | chr5.4:15327932-15327951 | None:intergenic | 35.0% |
| AAGTGATGGTCAAGCTATGA+AGG | + | chr5.4:15327739-15327758 | MS.gene67559:CDS | 40.0% | |
| ACAATGTCAAGTCAGGAAGA+GGG | + | chr5.4:15328442-15328461 | MS.gene67559:CDS | 40.0% | |
| ACCTCAACCTGATCAATATG+AGG | + | chr5.4:15327922-15327941 | MS.gene67559:CDS | 40.0% | |
| AGTGATGGTCAAGCTATGAA+GGG | + | chr5.4:15327740-15327759 | MS.gene67559:CDS | 40.0% | |
| CAAACATGTGTTTCCCACTT+GGG | + | chr5.4:15327793-15327812 | MS.gene67559:CDS | 40.0% | |
| CCTCAACCTGATCAATATGA+GGG | + | chr5.4:15327923-15327942 | MS.gene67559:CDS | 40.0% | |
| CTCTGATACAATGTCAAGTC+AGG | + | chr5.4:15328435-15328454 | MS.gene67559:CDS | 40.0% | |
| GAGCTCTTAAAGAACTCTGA+AGG | - | chr5.4:15327682-15327701 | None:intergenic | 40.0% | |
| TACAATGTCAAGTCAGGAAG+AGG | + | chr5.4:15328441-15328460 | MS.gene67559:CDS | 40.0% | |
| TCAGAGTCCTCTATCTCTAT+TGG | - | chr5.4:15328421-15328440 | None:intergenic | 40.0% | |
| TTCATCAAAGTAGCCCAAGT+GGG | - | chr5.4:15327809-15327828 | None:intergenic | 40.0% | |
| TTGAGGGATTCATCATCAGA+GGG | - | chr5.4:15327641-15327660 | None:intergenic | 40.0% | |
| TTTGAGGGATTCATCATCAG+AGG | - | chr5.4:15327642-15327661 | None:intergenic | 40.0% | |
| ! | AAAGAACTCTGAAGGGTAGT+TGG | - | chr5.4:15327674-15327693 | None:intergenic | 40.0% |
| ! | AAGAACTCTGAAGGGTAGTT+GGG | - | chr5.4:15327673-15327692 | None:intergenic | 40.0% |
| ! | CTTTAAGAGCTCGTGAGAAT+GGG | + | chr5.4:15327690-15327709 | MS.gene67559:CDS | 40.0% |
| ! | TCTTTAAGAGCTCGTGAGAA+TGG | + | chr5.4:15327689-15327708 | MS.gene67559:CDS | 40.0% |
| !! | AGTTGGGTTGGTTTCTTTGA+GGG | - | chr5.4:15327657-15327676 | None:intergenic | 40.0% |
| !! | ATATTGATCAGGTTGAGGTG+GGG | - | chr5.4:15327921-15327940 | None:intergenic | 40.0% |
| !! | CATATTGATCAGGTTGAGGT+GGG | - | chr5.4:15327922-15327941 | None:intergenic | 40.0% |
| !! | TAGTTGGGTTGGTTTCTTTG+AGG | - | chr5.4:15327658-15327677 | None:intergenic | 40.0% |
| !! | TCATATTGATCAGGTTGAGG+TGG | - | chr5.4:15327923-15327942 | None:intergenic | 40.0% |
| AAACAACAACACTGGACCAG+AGG | + | chr5.4:15328480-15328499 | MS.gene67559:CDS | 45.0% | |
| AAGCCAGCCAATAGAGATAG+AGG | + | chr5.4:15328411-15328430 | MS.gene67559:CDS | 45.0% | |
| AGTCCTCTATCTCTATTGGC+TGG | - | chr5.4:15328417-15328436 | None:intergenic | 45.0% | |
| CTTCATCAAAGTAGCCCAAG+TGG | - | chr5.4:15327810-15327829 | None:intergenic | 45.0% | |
| GCAAACATGTGTTTCCCACT+TGG | + | chr5.4:15327792-15327811 | MS.gene67559:CDS | 45.0% | |
| GCAATAAAACCCTAGGTCAC+TGG | - | chr5.4:15328033-15328052 | None:intergenic | 45.0% | |
| GTGATGGTCAAGCTATGAAG+GGG | + | chr5.4:15327741-15327760 | MS.gene67559:CDS | 45.0% | |
| GTTGAATTCTCGGAAGGGTT+AGG | - | chr5.4:15327587-15327606 | None:intergenic | 45.0% | |
| TCTCGGAAGGGTTAGGTTTA+GGG | - | chr5.4:15327580-15327599 | None:intergenic | 45.0% | |
| TTCTCGGAAGGGTTAGGTTT+AGG | - | chr5.4:15327581-15327600 | None:intergenic | 45.0% | |
| ! | CCCTCATATTGATCAGGTTG+AGG | - | chr5.4:15327926-15327945 | None:intergenic | 45.0% |
| ! | GAAGGAAGTGCAGAAAAACC+AGG | - | chr5.4:15327884-15327903 | None:intergenic | 45.0% |
| !!! | AATTTTTTTTTATAACTTAT+TGG | + | chr5.4:15328279-15328298 | MS.gene67559:intron | 5.0% |
| AACAACACTGGACCAGAGGA+AGG | + | chr5.4:15328484-15328503 | MS.gene67559:CDS | 50.0% | |
| AGGGATTCATCATCAGAGGG+AGG | - | chr5.4:15327638-15327657 | None:intergenic | 50.0% | |
| ATGGTCAAGCTATGAAGGGG+AGG | + | chr5.4:15327744-15327763 | MS.gene67559:CDS | 50.0% | |
| CAAGGCGCAAACAACAACAC+TGG | + | chr5.4:15328472-15328491 | MS.gene67559:CDS | 50.0% | |
| CATGGTTATTCAGCACCTCC+TGG | + | chr5.4:15327863-15327882 | MS.gene67559:CDS | 50.0% | |
| GATGGCGAAGGAGTAAGTGA+TGG | + | chr5.4:15327725-15327744 | MS.gene67559:CDS | 50.0% | |
| GGCTTCTTGATCGTCCTCTT+GGG | - | chr5.4:15328514-15328533 | None:intergenic | 50.0% | |
| GGGATTCATCATCAGAGGGA+GGG | - | chr5.4:15327637-15327656 | None:intergenic | 50.0% | |
| GTCAACACTCCAGTGACCTA+GGG | + | chr5.4:15328021-15328040 | MS.gene67559:intron | 50.0% | |
| TGGGGGTTGAATTCTCGGAA+GGG | - | chr5.4:15327592-15327611 | None:intergenic | 50.0% | |
| TGTCAAGTCAGGAAGAGGGA+AGG | + | chr5.4:15328446-15328465 | MS.gene67559:CDS | 50.0% | |
| ! | ACTCTGAAGGGTAGTTGGGT+TGG | - | chr5.4:15327669-15327688 | None:intergenic | 50.0% |
| ! | GGAAGTGCAGAAAAACCAGG+AGG | - | chr5.4:15327881-15327900 | None:intergenic | 50.0% |
| ! | GTTGGTGGGGGTTGAATTCT+CGG | - | chr5.4:15327597-15327616 | None:intergenic | 50.0% |
| !! | CAGTGTTGTTGTTTGCGCCT+TGG | - | chr5.4:15328474-15328493 | None:intergenic | 50.0% |
| !! | GGAGGTGCTGAATAACCATG+AGG | - | chr5.4:15327863-15327882 | None:intergenic | 50.0% |
| !! | GTCTTGAGGGGACTTGTTGT+TGG | - | chr5.4:15327615-15327634 | None:intergenic | 50.0% |
| !! | TGAGGGGACTTGTTGTTGGT+GGG | - | chr5.4:15327611-15327630 | None:intergenic | 50.0% |
| !! | TTGAGGGGACTTGTTGTTGG+TGG | - | chr5.4:15327612-15327631 | None:intergenic | 50.0% |
| AATGGGAAGCTGGCAGCTGA+TGG | + | chr5.4:15327707-15327726 | MS.gene67559:CDS | 55.0% | |
| ACACTGGACCAGAGGAAGGA+AGG | + | chr5.4:15328488-15328507 | MS.gene67559:CDS | 55.0% | |
| AGCTCGTGAGAATGGGAAGC+TGG | + | chr5.4:15327697-15327716 | MS.gene67559:CDS | 55.0% | |
| ATCAGAGGGAGGGTCTTGAG+GGG | - | chr5.4:15327627-15327646 | None:intergenic | 55.0% | |
| CAGGAAGAGGGAAGGAACCA+AGG | + | chr5.4:15328454-15328473 | MS.gene67559:CDS | 55.0% | |
| CATCAGAGGGAGGGTCTTGA+GGG | - | chr5.4:15327628-15327647 | None:intergenic | 55.0% | |
| GGGCTTCTTGATCGTCCTCT+TGG | - | chr5.4:15328515-15328534 | None:intergenic | 55.0% | |
| GGTCAACACTCCAGTGACCT+AGG | + | chr5.4:15328020-15328039 | MS.gene67559:intron | 55.0% | |
| GTGGGGGTTGAATTCTCGGA+AGG | - | chr5.4:15327593-15327612 | None:intergenic | 55.0% | |
| TCATCAGAGGGAGGGTCTTG+AGG | - | chr5.4:15327629-15327648 | None:intergenic | 55.0% | |
| !! | AGGGGACTTGTTGTTGGTGG+GGG | - | chr5.4:15327609-15327628 | None:intergenic | 55.0% |
| !! | GAGGGGACTTGTTGTTGGTG+GGG | - | chr5.4:15327610-15327629 | None:intergenic | 55.0% |
| !! | TTGATCAGGTTGAGGTGGGG+TGG | - | chr5.4:15327918-15327937 | None:intergenic | 55.0% |
| AGGTGGGGTGGCTGTTGATG+TGG | - | chr5.4:15327906-15327925 | None:intergenic | 60.0% | |
| CAGAGGAAGGAAGGCCCAAG+AGG | + | chr5.4:15328497-15328516 | MS.gene67559:CDS | 60.0% | |
| CTCTTGGGCCTTCCTTCCTC+TGG | - | chr5.4:15328499-15328518 | None:intergenic | 60.0% | |
| GGGGTGGCTGTTGATGTGGA+AGG | - | chr5.4:15327902-15327921 | None:intergenic | 60.0% | |
| ! | AAGCTGGCAGCTGATGGCGA+AGG | + | chr5.4:15327713-15327732 | MS.gene67559:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 15327569 | 15328549 | 15327569 | ID=MS.gene67559 |
| chr5.4 | mRNA | 15327569 | 15328549 | 15327569 | ID=MS.gene67559.t1;Parent=MS.gene67559 |
| chr5.4 | exon | 15327569 | 15327944 | 15327569 | ID=MS.gene67559.t1.exon1;Parent=MS.gene67559.t1 |
| chr5.4 | CDS | 15327569 | 15327944 | 15327569 | ID=cds.MS.gene67559.t1;Parent=MS.gene67559.t1 |
| chr5.4 | exon | 15328401 | 15328549 | 15328401 | ID=MS.gene67559.t1.exon2;Parent=MS.gene67559.t1 |
| chr5.4 | CDS | 15328401 | 15328549 | 15328401 | ID=cds.MS.gene67559.t1;Parent=MS.gene67559.t1 |
| Gene Sequence |
| Protein sequence |
