AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67838

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67838.t1 MTR_8g069935 51.575 254 109 5 6 255 220 463 8.09e-80 248
MS.gene67838.t1 MTR_2g024380 43.284 335 81 5 1 255 165 470 1.18e-78 245
MS.gene67838.t1 MTR_8g067635 42.434 304 123 4 1 254 234 535 2.00e-66 215
MS.gene67838.t1 MTR_8g067650 40.924 303 119 5 3 254 226 519 2.87e-65 211
MS.gene67838.t1 MTR_6g053200 36.991 319 118 5 5 255 233 536 1.98e-59 197
MS.gene67838.t1 MTR_2g024350 40.969 227 54 3 109 255 1 227 3.18e-49 162
MS.gene67838.t1 MTR_7g105000 50.735 136 63 3 2 136 208 340 2.94e-33 124
MS.gene67838.t1 MTR_1g099600 46.602 103 53 2 88 190 158 258 6.58e-26 103
MS.gene67838.t1 MTR_8g028565 42.857 112 57 3 93 204 134 238 1.08e-22 95.5
MS.gene67838.t1 MTR_7g079040 44.660 103 54 2 90 192 130 229 3.57e-22 93.6
MS.gene67838.t1 MTR_2g032030 38.017 121 63 3 81 190 221 340 4.04e-20 89.0
MS.gene67838.t1 MTR_2g032030 38.017 121 63 3 81 190 221 340 8.70e-20 88.6
MS.gene67838.t1 MTR_8g087000 44.048 84 40 1 116 199 77 153 3.72e-19 84.0
MS.gene67838.t1 MTR_4g127040 43.373 83 44 1 94 173 220 302 4.00e-19 85.1
MS.gene67838.t1 MTR_8g087000 44.048 84 40 1 116 199 76 152 4.54e-19 84.0
MS.gene67838.t1 MTR_5g016610 43.373 83 44 1 94 173 174 256 1.05e-18 83.2
MS.gene67838.t1 MTR_7g109600 38.462 117 59 2 67 176 3 113 1.22e-18 83.2
MS.gene67838.t1 MTR_8g098945 37.719 114 69 2 117 230 130 241 1.79e-18 82.8
MS.gene67838.t1 MTR_4g094908 40.741 108 57 1 73 173 216 323 1.93e-18 83.6
MS.gene67838.t1 MTR_2g016770 34.899 149 89 5 88 230 2 148 2.81e-18 81.3
MS.gene67838.t1 MTR_2g016770 38.793 116 68 3 116 230 27 140 6.01e-18 80.1
MS.gene67838.t1 MTR_2g083870 54.098 61 28 0 116 176 80 140 1.41e-17 80.1
MS.gene67838.t1 MTR_7g104980 34.010 197 51 3 138 255 19 215 1.93e-17 79.0
MS.gene67838.t1 MTR_1g070455 43.373 83 46 1 93 175 271 352 2.20e-17 80.9
MS.gene67838.t1 MTR_1g009613 40.741 81 47 1 93 173 240 319 7.36e-17 79.0
MS.gene67838.t1 MTR_4g130900 52.381 63 29 1 114 176 357 418 1.59e-16 79.0
MS.gene67838.t1 MTR_3g108960 41.250 80 46 1 95 173 292 371 3.24e-16 77.8
MS.gene67838.t1 MTR_0117s0040 47.692 65 34 0 110 174 68 132 7.74e-16 75.1
MS.gene67838.t1 MTR_7g011820 47.222 72 37 1 105 176 399 469 1.86e-15 75.9
MS.gene67838.t1 MTR_7g011820 46.970 66 33 2 112 177 224 287 1.68e-12 67.0
MS.gene67838.t1 MTR_5g094430 36.364 132 71 6 105 231 353 476 1.94e-15 75.9
MS.gene67838.t1 MTR_5g094430 44.444 72 38 2 115 186 199 268 1.35e-11 64.3
MS.gene67838.t1 MTR_5g094430 36.364 132 71 6 105 231 219 342 2.96e-15 75.1
MS.gene67838.t1 MTR_5g094430 44.444 72 38 2 115 186 65 134 1.71e-11 63.9
MS.gene67838.t1 MTR_5g094430 36.364 132 71 6 105 231 241 364 3.18e-15 75.1
MS.gene67838.t1 MTR_5g094430 44.444 72 38 2 115 186 87 156 1.76e-11 63.9
MS.gene67838.t1 MTR_7g100510 52.632 76 34 2 112 187 185 258 3.31e-15 75.1
MS.gene67838.t1 MTR_7g100510 42.424 66 37 1 111 176 356 420 7.04e-14 70.9
MS.gene67838.t1 MTR_3g056100 43.056 72 40 1 105 176 391 461 1.18e-14 73.6
MS.gene67838.t1 MTR_3g056100 37.391 115 64 4 112 225 239 346 6.70e-13 68.2
MS.gene67838.t1 MTR_3g056100 43.056 72 40 1 105 176 404 474 1.22e-14 73.6
MS.gene67838.t1 MTR_3g056100 39.000 100 54 3 112 211 239 331 8.00e-13 68.2
MS.gene67838.t1 MTR_3g056100 43.056 72 40 1 105 176 260 330 1.57e-14 73.2
MS.gene67838.t1 MTR_3g056100 39.000 100 54 3 112 211 95 187 8.40e-13 67.8
MS.gene67838.t1 MTR_3g056100 43.056 72 40 1 105 176 247 317 1.57e-14 72.8
MS.gene67838.t1 MTR_3g056100 37.391 115 64 4 112 225 95 202 7.78e-13 67.8
MS.gene67838.t1 MTR_5g097490 45.833 72 38 1 105 176 241 311 2.49e-14 72.0
MS.gene67838.t1 MTR_3g009470 41.758 91 49 3 115 204 279 366 3.60e-14 72.0
MS.gene67838.t1 MTR_3g009470 35.354 99 52 2 89 176 457 554 2.45e-12 66.6
MS.gene67838.t1 MTR_3g009470 41.758 91 49 3 115 204 279 366 3.76e-14 72.0
MS.gene67838.t1 MTR_7g080020 31.148 122 69 3 74 181 437 557 3.99e-14 72.0
MS.gene67838.t1 MTR_5g042920 38.889 90 54 1 105 194 309 397 4.04e-14 71.6
MS.gene67838.t1 MTR_5g042920 49.231 65 31 2 112 176 147 209 2.33e-11 63.5
MS.gene67838.t1 MTR_3g053330 31.034 145 88 3 57 201 203 335 4.21e-14 71.6
MS.gene67838.t1 MTR_2g045360 43.077 65 37 0 116 180 211 275 9.88e-14 70.5
MS.gene67838.t1 MTR_2g088000 35.294 119 71 3 83 201 321 433 1.13e-13 70.5
MS.gene67838.t1 MTR_1g013790 27.778 162 100 4 22 178 14 163 2.26e-13 68.2
MS.gene67838.t1 MTR_5g074400 45.312 64 34 1 113 176 417 479 2.63e-13 69.7
MS.gene67838.t1 MTR_5g074400 52.381 63 28 2 115 177 242 302 2.71e-12 66.6
MS.gene67838.t1 MTR_1g086790 36.667 90 55 2 96 185 139 226 3.15e-13 68.6
MS.gene67838.t1 MTR_3g106060 45.614 57 31 0 117 173 139 195 3.22e-13 68.2
MS.gene67838.t1 MTR_4g132430 30.534 131 82 3 52 176 194 321 3.71e-13 68.9
MS.gene67838.t1 MTR_4g132430 33.333 105 67 2 72 176 86 187 4.34e-13 68.6
MS.gene67838.t1 MTR_4g007060 41.538 65 38 0 117 181 129 193 4.49e-13 67.8
MS.gene67838.t1 MTR_4g007060 41.538 65 38 0 117 181 163 227 5.93e-13 67.8
MS.gene67838.t1 MTR_3g031220 43.750 64 35 1 113 176 362 424 7.39e-13 68.2
MS.gene67838.t1 MTR_3g031220 51.613 62 28 2 115 176 197 256 7.67e-12 65.1
MS.gene67838.t1 MTR_4g049790 34.940 83 54 0 111 193 307 389 8.19e-13 68.2
MS.gene67838.t1 MTR_7g109800 37.805 82 49 2 95 176 125 204 1.04e-12 67.0
MS.gene67838.t1 MTR_4g082580 43.750 64 35 1 113 176 275 337 1.12e-12 67.4
MS.gene67838.t1 MTR_7g071120 34.507 142 78 5 108 240 158 293 1.18e-12 67.4
MS.gene67838.t1 MTR_7g110720 34.043 94 61 1 99 192 173 265 1.34e-12 67.0
MS.gene67838.t1 MTR_7g110720 37.179 78 48 1 99 176 200 276 1.60e-12 67.0
MS.gene67838.t1 MTR_4g049760 34.940 83 54 0 111 193 285 367 1.65e-12 67.0
MS.gene67838.t1 MTR_2g023930 34.000 100 62 3 78 176 269 365 1.75e-12 67.0
MS.gene67838.t1 MTR_7g080000 37.037 81 50 1 96 176 412 491 2.39e-12 66.6
MS.gene67838.t1 MTR_4g052030 52.727 55 14 1 119 173 8 50 3.03e-12 60.5
MS.gene67838.t1 MTR_5g043880 35.294 102 64 2 72 173 81 180 5.00e-12 64.7
MS.gene67838.t1 MTR_7g062220 42.667 75 39 2 114 188 122 192 5.06e-12 65.1
MS.gene67838.t1 MTR_3g031220 51.613 62 28 2 115 176 197 256 6.78e-12 65.1
MS.gene67838.t1 MTR_1g013760 43.860 57 32 0 117 173 141 197 6.80e-12 64.3
MS.gene67838.t1 MTR_4g013260 42.424 66 37 1 111 176 331 395 9.02e-12 64.7
MS.gene67838.t1 MTR_2g105060 37.805 82 47 1 101 178 75 156 1.36e-11 63.2
MS.gene67838.t1 MTR_4g107970 37.179 78 47 1 101 176 161 238 1.43e-11 64.3
MS.gene67838.t1 MTR_2g105060 37.805 82 47 1 101 178 150 231 1.77e-11 63.5
MS.gene67838.t1 MTR_4g094588 37.662 77 45 2 106 180 166 241 1.92e-11 63.5
MS.gene67838.t1 MTR_2g033820 45.000 60 32 1 117 176 204 262 1.99e-11 63.5
MS.gene67838.t1 MTR_4g107970 37.179 78 47 1 101 176 238 315 2.04e-11 63.9
MS.gene67838.t1 MTR_7g009730 30.973 113 73 3 61 173 169 276 2.53e-11 63.5
MS.gene67838.t1 MTR_4g094412 30.357 112 63 2 65 176 198 294 3.68e-11 63.2
MS.gene67838.t1 MTR_4g094412 30.357 112 63 2 65 176 207 303 4.31e-11 62.8
MS.gene67838.t1 MTR_1g015140 44.262 61 33 1 113 173 99 158 4.71e-11 60.1
MS.gene67838.t1 MTR_5g091390 41.538 65 37 1 117 181 158 221 5.93e-11 62.0
MS.gene67838.t1 MTR_4g122530 37.681 69 42 1 117 185 209 276 7.87e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67838.t1 AT5G52830 41.892 148 71 6 93 230 143 285 4.62e-26 104
MS.gene67838.t1 AT4G01250 31.401 207 129 6 56 254 72 273 4.34e-25 101
MS.gene67838.t1 AT5G45050 36.441 118 73 2 93 209 1017 1133 6.43e-19 86.3
MS.gene67838.t1 AT5G45050 36.441 118 73 2 93 209 1156 1272 6.98e-19 86.3
MS.gene67838.t1 AT5G45050 36.441 118 73 2 93 209 1128 1244 7.87e-19 86.3
MS.gene67838.t1 AT2G34830 38.333 120 61 4 75 185 168 283 9.93e-19 85.1
MS.gene67838.t1 AT2G34830 38.333 120 61 4 75 185 177 292 1.14e-18 85.1
MS.gene67838.t1 AT4G23550 36.434 129 69 4 100 216 124 251 2.94e-18 82.8
MS.gene67838.t1 AT2G24570 44.444 81 44 1 93 173 221 300 3.56e-18 82.8
MS.gene67838.t1 AT1G29280 42.553 94 51 1 86 176 41 134 3.86e-18 81.6
MS.gene67838.t1 AT5G28650 44.706 85 44 1 94 175 237 321 4.75e-18 82.4
MS.gene67838.t1 AT4G31550 43.210 81 45 1 93 173 224 303 5.51e-18 82.0
MS.gene67838.t1 AT4G31550 43.902 82 43 1 95 173 221 302 5.62e-18 82.0
MS.gene67838.t1 AT1G30650 40.385 104 51 3 91 185 184 285 5.64e-18 83.2
MS.gene67838.t1 AT3G04670 44.706 85 44 1 94 175 237 321 7.87e-18 81.6
MS.gene67838.t1 AT2G23320 54.386 57 26 0 117 173 241 297 1.20e-17 81.3
MS.gene67838.t1 AT4G24240 40.860 93 52 1 84 173 246 338 1.45e-17 81.3
MS.gene67838.t1 AT3G58710 48.485 66 34 0 116 181 70 135 9.70e-17 77.8
MS.gene67838.t1 AT3G58710 48.485 66 34 0 116 181 71 136 1.01e-16 77.8
MS.gene67838.t1 AT5G07100 35.294 136 80 5 44 176 70 200 2.06e-16 76.3
MS.gene67838.t1 AT5G07100 43.056 72 40 1 109 180 17 87 1.53e-11 62.8
MS.gene67838.t1 AT5G07100 35.294 136 80 5 44 176 70 200 2.06e-16 76.3
MS.gene67838.t1 AT5G07100 43.056 72 40 1 109 180 17 87 1.53e-11 62.8
MS.gene67838.t1 AT5G07100 35.294 136 80 5 44 176 70 200 2.06e-16 76.3
MS.gene67838.t1 AT5G07100 43.056 72 40 1 109 180 17 87 1.53e-11 62.8
MS.gene67838.t1 AT5G07100 35.294 136 80 5 44 176 70 200 2.06e-16 76.3
MS.gene67838.t1 AT5G07100 43.056 72 40 1 109 180 17 87 1.53e-11 62.8
MS.gene67838.t1 AT4G30935 41.837 98 52 3 80 176 297 390 4.29e-16 77.8
MS.gene67838.t1 AT5G07100 34.028 144 87 5 44 184 163 301 4.53e-16 76.6
MS.gene67838.t1 AT5G07100 43.056 72 40 1 109 180 110 180 1.13e-11 63.9
MS.gene67838.t1 AT2G30590 41.176 85 47 1 94 175 288 372 8.98e-16 76.3
MS.gene67838.t1 AT3G01080 48.485 66 33 1 111 176 339 403 2.04e-15 75.5
MS.gene67838.t1 AT3G01080 45.455 66 34 2 112 177 201 264 8.51e-13 67.8
MS.gene67838.t1 AT3G01080 48.485 66 33 1 111 176 301 365 2.49e-15 75.5
MS.gene67838.t1 AT3G01080 45.455 66 34 2 112 177 163 226 1.01e-12 67.8
MS.gene67838.t1 AT2G04880 36.434 129 62 5 78 192 231 353 3.29e-15 75.1
MS.gene67838.t1 AT2G04880 36.434 129 62 5 78 192 255 377 3.35e-15 75.1
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 194 264 1.01e-14 73.2
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 33 110 8.79e-11 61.6
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 194 264 1.01e-14 73.2
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 33 110 8.79e-11 61.6
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 194 264 1.01e-14 73.2
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 33 110 8.79e-11 61.6
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 194 264 1.01e-14 73.2
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 33 110 8.79e-11 61.6
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 330 400 1.07e-14 73.6
MS.gene67838.t1 AT4G26640 48.438 64 31 2 114 177 169 230 8.90e-11 62.0
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 370 440 1.18e-14 73.6
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 209 286 8.77e-11 62.0
MS.gene67838.t1 AT4G26640 43.056 72 40 1 105 176 298 368 1.29e-14 73.2
MS.gene67838.t1 AT4G26640 41.250 80 45 2 114 193 137 214 9.83e-11 62.0
MS.gene67838.t1 AT1G69810 42.308 78 44 1 111 187 140 217 3.02e-14 71.6
MS.gene67838.t1 AT2G37260 42.683 82 42 2 96 173 325 405 3.16e-14 72.0
MS.gene67838.t1 AT2G37260 50.769 65 30 2 112 176 161 223 1.78e-11 63.9
MS.gene67838.t1 AT2G37260 42.683 82 42 2 96 173 245 325 3.89e-14 71.6
MS.gene67838.t1 AT2G37260 50.769 65 30 2 112 176 81 143 2.51e-11 63.2
MS.gene67838.t1 AT1G69810 42.308 78 44 1 111 187 198 275 3.91e-14 71.6
MS.gene67838.t1 AT2G37260 42.683 82 42 2 96 173 243 323 4.04e-14 71.2
MS.gene67838.t1 AT2G37260 50.769 65 30 2 112 176 79 141 2.69e-11 63.2
MS.gene67838.t1 AT2G03340 41.667 72 41 1 105 176 404 474 5.31e-14 71.6
MS.gene67838.t1 AT2G03340 45.714 70 36 2 112 181 246 313 1.92e-11 63.9
MS.gene67838.t1 AT1G13960 41.667 72 41 1 105 176 398 468 5.57e-14 71.6
MS.gene67838.t1 AT1G13960 44.928 69 36 2 112 180 225 291 7.43e-12 65.1
MS.gene67838.t1 AT1G13960 41.667 72 41 1 105 176 245 315 1.04e-13 70.5
MS.gene67838.t1 AT1G13960 44.928 69 36 2 112 180 72 138 1.57e-11 63.9
MS.gene67838.t1 AT2G38470 51.471 68 31 2 110 177 178 243 2.09e-13 69.7
MS.gene67838.t1 AT2G38470 46.667 60 31 1 117 176 363 421 2.37e-13 69.7
MS.gene67838.t1 AT4G04450 34.940 83 54 0 111 193 193 275 2.35e-13 69.7
MS.gene67838.t1 AT4G04450 34.940 83 54 0 111 193 287 369 3.88e-13 68.9
MS.gene67838.t1 AT3G01080 45.455 66 34 2 112 177 201 264 6.33e-13 67.8
MS.gene67838.t1 AT2G30250 39.474 76 45 1 105 180 317 391 8.21e-13 67.8
MS.gene67838.t1 AT5G26170 47.619 63 32 1 111 173 108 169 1.00e-12 65.1
MS.gene67838.t1 AT1G80840 37.805 82 47 1 102 179 128 209 1.18e-12 67.0
MS.gene67838.t1 AT1G69310 34.000 100 64 2 75 174 107 204 1.35e-12 66.6
MS.gene67838.t1 AT1G69310 34.000 100 64 2 75 174 107 204 1.35e-12 66.6
MS.gene67838.t1 AT1G69310 34.000 100 64 2 75 174 107 204 1.35e-12 66.6
MS.gene67838.t1 AT2G47260 39.024 82 48 2 95 176 154 233 1.41e-12 67.0
MS.gene67838.t1 AT4G22070 37.879 66 41 0 111 176 292 357 1.69e-12 67.0
MS.gene67838.t1 AT4G22070 37.879 66 41 0 111 176 292 357 1.69e-12 67.0
MS.gene67838.t1 AT4G22070 37.879 66 41 0 111 176 292 357 1.69e-12 67.0
MS.gene67838.t1 AT4G22070 37.879 66 41 0 111 176 292 357 1.69e-12 67.0
MS.gene67838.t1 AT4G26440 40.964 83 47 2 117 199 179 259 2.03e-12 67.0
MS.gene67838.t1 AT1G62300 37.879 66 41 0 111 176 307 372 5.24e-12 65.9
MS.gene67838.t1 AT4G18170 36.047 86 53 2 96 181 153 236 8.99e-12 64.3
MS.gene67838.t1 AT1G29860 37.805 82 48 2 106 185 124 204 9.47e-12 63.9
MS.gene67838.t1 AT4G31800 40.000 60 36 0 117 176 112 171 1.66e-11 62.8
MS.gene67838.t1 AT5G56270 43.333 60 33 1 117 176 488 546 1.68e-11 64.3
MS.gene67838.t1 AT5G56270 50.000 60 28 2 117 176 274 331 3.44e-11 63.5
MS.gene67838.t1 AT5G43290 48.333 60 30 1 114 173 112 170 1.90e-11 63.2
MS.gene67838.t1 AT4G31800 40.000 60 36 0 117 176 177 236 2.12e-11 63.2
MS.gene67838.t1 AT1G55600 31.461 89 60 1 88 176 279 366 2.25e-11 63.9
MS.gene67838.t1 AT1G18860 41.667 60 35 0 117 176 240 299 3.97e-11 63.2
MS.gene67838.t1 AT5G49520 37.179 78 47 2 99 176 205 280 4.22e-11 62.8
MS.gene67838.t1 AT5G15130 36.364 77 47 1 102 176 169 245 7.12e-11 62.4
MS.gene67838.t1 AT5G45260 45.161 62 32 2 117 176 1209 1270 7.46e-11 62.4
MS.gene67838.t1 AT5G15130 36.364 77 47 1 102 176 211 287 8.64e-11 62.0

Find 38 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAACAGACTTTAT+TGG 0.122401 2.4:+57382727 MS.gene67838:CDS
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2.4:+57381323 MS.gene67838:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2.4:-57382613 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2.4:-57381330 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2.4:-57381316 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2.4:+57381374 MS.gene67838:CDS
GACTTTATTGGAAACTCTAA+TGG 0.367637 2.4:+57382739 MS.gene67838:CDS
TCTGTCAAAAGATTTATTGA+TGG 0.375523 2.4:+57382795 MS.gene67838:CDS
GCTTATATTGTGACGTATAA+AGG 0.379319 2.4:+57382571 MS.gene67838:CDS
TTTGACAGAGTTGTCATGTT+TGG 0.387367 2.4:-57382781 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2.4:-57381329 None:intergenic
ACAAACACTAGGATATTGAT+TGG 0.431548 2.4:-57381131 None:intergenic
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2.4:+57382619 MS.gene67838:CDS
GATTGTTGCGGATTTGTGTT+TGG 0.435515 2.4:-57381352 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2.4:-57381277 None:intergenic
GACTTTAACCACCTCAATAG+TGG 0.457259 2.4:+57382817 MS.gene67838:CDS
ACCTGAGCATTGCTAGTCTC+TGG 0.478042 2.4:-57382679 None:intergenic
TGAATCTGTTGTTGTTCAAC+GGG 0.491849 2.4:-57381166 None:intergenic
ATAACACACCAAGGACATCT+TGG 0.504754 2.4:-57381539 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2.4:-57381286 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2.4:+57381324 MS.gene67838:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.509380 2.4:+57381545 MS.gene67838:CDS
GAAATAAAGCACCACTATTG+AGG 0.510102 2.4:-57382828 None:intergenic
ATGAATCTGTTGTTGTTCAA+CGG 0.520706 2.4:-57381167 None:intergenic
GCCAGAGACTAGCAATGCTC+AGG 0.528932 2.4:+57382678 MS.gene67838:CDS
AAAGAATGCCAAGATGTCCT+TGG 0.553216 2.4:+57381531 MS.gene67838:CDS
TTGAAGGGTCTCCACATCCA+AGG 0.557586 2.4:+57381623 MS.gene67838:CDS
TATGTTGTGGACTAGATGGA+TGG 0.570891 2.4:-57381273 None:intergenic
TGAACTTTCAACAGACTCAT+GGG 0.572196 2.4:+57381573 MS.gene67838:CDS
ATGTATGAAAACCTTGGATG+TGG 0.583670 2.4:-57381634 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2.4:+57381572 MS.gene67838:CDS
TCATGGGCATGGAGTAAACA+TGG 0.611520 2.4:+57381589 MS.gene67838:CDS
TTTCAACAGACTCATGGGCA+TGG 0.620196 2.4:+57381578 MS.gene67838:CDS
GATCTGAGGTTTGATTGTTG+CGG 0.622260 2.4:-57381364 None:intergenic
ATAAAGCACCACTATTGAGG+TGG 0.656429 2.4:-57382825 None:intergenic
TTCCCTCATATAACACACCA+AGG 0.662868 2.4:-57381548 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.673519 2.4:+57381546 MS.gene67838:CDS
GTATGCACGATCAAAATGAG+CGG 0.723045 2.4:-57381248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAACAATTGATA+CGG + chr2.4:57382249-57382268 MS.gene67838:intron 10.0%
!! ATTAAATATTCTACATATTG+AGG + chr2.4:57381964-57381983 MS.gene67838:intron 15.0%
!! GGATTAAAATGAATATTATA+TGG - chr2.4:57381926-57381945 None:intergenic 15.0%
!! TAATATGAATAATATTTGCA+GGG + chr2.4:57381996-57382015 MS.gene67838:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG + chr2.4:57381995-57382014 MS.gene67838:intron 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG - chr2.4:57381827-57381846 None:intergenic 15.0%
!!! ATAGAAAAAGTAATTTTACA+GGG - chr2.4:57381826-57381845 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG + chr2.4:57381936-57381955 MS.gene67838:intron 15.0%
!!! CAATTTCAATATTTTTTCTT+AGG - chr2.4:57381901-57381920 None:intergenic 15.0%
!!! CTATATATATATTTTGTCTT+TGG - chr2.4:57382067-57382086 None:intergenic 15.0%
!! AAAGTGAAAGATATAAACTT+AGG - chr2.4:57382186-57382205 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG + chr2.4:57382220-57382239 MS.gene67838:intron 20.0%
!!! ATAGGACATGTTTTATATAA+AGG - chr2.4:57382376-57382395 None:intergenic 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG + chr2.4:57381935-57381954 MS.gene67838:intron 20.0%
!!! TTTATATAAAGGTATGCAAT+CGG - chr2.4:57382365-57382384 None:intergenic 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG - chr2.4:57381514-57381533 None:intergenic 20.0%
! ACAAAGTTCTTAATTTCTTC+TGG - chr2.4:57382616-57382635 None:intergenic 25.0%
! CACACATTACACATATATAT+AGG - chr2.4:57382394-57382413 None:intergenic 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG - chr2.4:57381506-57381525 None:intergenic 25.0%
! TCTGTCAAAAGATTTATTGA+TGG + chr2.4:57382795-57382814 MS.gene67838:CDS 25.0%
!! AAATAGTGACAGTAAACTTT+TGG + chr2.4:57381748-57381767 MS.gene67838:intron 25.0%
!! AATTTTTGCCCATCAAAATA+AGG - chr2.4:57381947-57381966 None:intergenic 25.0%
!! GAAATCTATGAATGATTTGT+AGG + chr2.4:57382473-57382492 MS.gene67838:intron 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG + chr2.4:57382619-57382638 MS.gene67838:CDS 25.0%
!! TTTTGAATGTATGAAAACCT+TGG - chr2.4:57381643-57381662 None:intergenic 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG - chr2.4:57382710-57382729 None:intergenic 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG - chr2.4:57382609-57382628 None:intergenic 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG - chr2.4:57382532-57382551 None:intergenic 25.0%
ACAAACACTAGGATATTGAT+TGG - chr2.4:57381134-57381153 None:intergenic 30.0%
ACACACATATATGTCATGAT+GGG + chr2.4:57382089-57382108 MS.gene67838:intron 30.0%
ATCCTTGCAATTTCATAGAA+TGG + chr2.4:57381771-57381790 MS.gene67838:intron 30.0%
ATGGACAAAAATTCGTTGAA+GGG + chr2.4:57381608-57381627 MS.gene67838:CDS 30.0%
GACTTTATTGGAAACTCTAA+TGG + chr2.4:57382739-57382758 MS.gene67838:CDS 30.0%
GCTTATATTGTGACGTATAA+AGG + chr2.4:57382571-57382590 MS.gene67838:CDS 30.0%
GTTGAAAAATCACAAACACT+AGG - chr2.4:57381145-57381164 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG + chr2.4:57382034-57382053 MS.gene67838:intron 30.0%
TACCATTCTATGAAATTGCA+AGG - chr2.4:57381776-57381795 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG - chr2.4:57381735-57381754 None:intergenic 30.0%
! ATGAATCTGTTGTTGTTCAA+CGG - chr2.4:57381170-57381189 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG - chr2.4:57381381-57381400 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG - chr2.4:57381734-57381753 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG - chr2.4:57381740-57381759 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG - chr2.4:57382050-57382069 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG - chr2.4:57381478-57381497 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG - chr2.4:57381332-57381351 None:intergenic 30.0%
!! TGTTCTGAAACAGACTTTAT+TGG + chr2.4:57382727-57382746 MS.gene67838:CDS 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG - chr2.4:57382043-57382062 None:intergenic 30.0%
AAGAAATATGCCACTCATTG+TGG + chr2.4:57382332-57382351 MS.gene67838:intron 35.0%
AGAAATATGCCACTCATTGT+GGG + chr2.4:57382333-57382352 MS.gene67838:intron 35.0%
AGGAACTATAACAAACACCA+GGG - chr2.4:57381881-57381900 None:intergenic 35.0%
ATGAACTTTCAACAGACTCA+TGG + chr2.4:57381572-57381591 MS.gene67838:CDS 35.0%
ATGTATGAAAACCTTGGATG+TGG - chr2.4:57381637-57381656 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG + chr2.4:57381374-57381393 MS.gene67838:CDS 35.0%
CACACACATATATGTCATGA+TGG + chr2.4:57382088-57382107 MS.gene67838:intron 35.0%
CATGGACAAAAATTCGTTGA+AGG + chr2.4:57381607-57381626 MS.gene67838:CDS 35.0%
CATTGCAAATATTCCACCTT+TGG + chr2.4:57381847-57381866 MS.gene67838:intron 35.0%
GTAAAACCCTAGAACTAGTT+TGG - chr2.4:57381333-57381352 None:intergenic 35.0%
TAGGAACTATAACAAACACC+AGG - chr2.4:57381882-57381901 None:intergenic 35.0%
TGAACTTTCAACAGACTCAT+GGG + chr2.4:57381573-57381592 MS.gene67838:CDS 35.0%
TTGCTTCTTTGTGTATGTTG+TGG - chr2.4:57381289-57381308 None:intergenic 35.0%
TTTGACAGAGTTGTCATGTT+TGG - chr2.4:57382784-57382803 None:intergenic 35.0%
! GAAATAAAGCACCACTATTG+AGG - chr2.4:57382831-57382850 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG + chr2.4:57381323-57381342 MS.gene67838:CDS 35.0%
! TGAATCTGTTGTTGTTCAAC+GGG - chr2.4:57381169-57381188 None:intergenic 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG + chr2.4:57381324-57381343 MS.gene67838:CDS 35.0%
!! CAGCATCATTTTGTTATGTG+AGG - chr2.4:57382647-57382666 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG - chr2.4:57381319-57381338 None:intergenic 35.0%
AAAGAATGCCAAGATGTCCT+TGG + chr2.4:57381531-57381550 MS.gene67838:CDS 40.0%
ATAACACACCAAGGACATCT+TGG - chr2.4:57381542-57381561 None:intergenic 40.0%
GACTTTAACCACCTCAATAG+TGG + chr2.4:57382817-57382836 MS.gene67838:CDS 40.0%
GATTGTTGCGGATTTGTGTT+TGG - chr2.4:57381355-57381374 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG - chr2.4:57381251-57381270 None:intergenic 40.0%
TTCCCTCATATAACACACCA+AGG - chr2.4:57381551-57381570 None:intergenic 40.0%
! ATAAAGCACCACTATTGAGG+TGG - chr2.4:57382828-57382847 None:intergenic 40.0%
! GATCTGAGGTTTGATTGTTG+CGG - chr2.4:57381367-57381386 None:intergenic 40.0%
! GTCCTTGGTGTGTTATATGA+GGG + chr2.4:57381546-57381565 MS.gene67838:CDS 40.0%
! TGTCCTTGGTGTGTTATATG+AGG + chr2.4:57381545-57381564 MS.gene67838:CDS 40.0%
!! TATGTTGTGGACTAGATGGA+TGG - chr2.4:57381276-57381295 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG - chr2.4:57381280-57381299 None:intergenic 40.0%
ATATGCCACTCATTGTGGGA+CGG + chr2.4:57382337-57382356 MS.gene67838:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG - chr2.4:57381866-57381885 None:intergenic 45.0%
CAGGGACAAAAAACCAAAGG+TGG - chr2.4:57381863-57381882 None:intergenic 45.0%
TCATGGGCATGGAGTAAACA+TGG + chr2.4:57381589-57381608 MS.gene67838:CDS 45.0%
TTTCAACAGACTCATGGGCA+TGG + chr2.4:57381578-57381597 MS.gene67838:CDS 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG + chr2.4:57381861-57381880 MS.gene67838:intron 45.0%
!! AATATTATTCATATTAAAAT+TGG - chr2.4:57381992-57382011 None:intergenic 5.0%
!! ATTAAAATATTACAATTTAA+TGG + chr2.4:57382435-57382454 MS.gene67838:intron 5.0%
GTGGTGTGGGTGTGTGTATT+AGG - chr2.4:57381721-57381740 None:intergenic 50.0%
TTGAAGGGTCTCCACATCCA+AGG + chr2.4:57381623-57381642 MS.gene67838:CDS 50.0%
! ACCTGAGCATTGCTAGTCTC+TGG - chr2.4:57382682-57382701 None:intergenic 50.0%
GCCAGAGACTAGCAATGCTC+AGG + chr2.4:57382678-57382697 MS.gene67838:CDS 55.0%
GTAATGTGTGCGCGCGCAAA+TGG + chr2.4:57382404-57382423 MS.gene67838:intron 55.0%
CGGTTCCGTCCCACAATGAG+TGG - chr2.4:57382345-57382364 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 57381106 57382855 57381106 ID=MS.gene67838
chr2.4 mRNA 57381106 57382855 57381106 ID=MS.gene67838.t1;Parent=MS.gene67838
chr2.4 exon 57381106 57381395 57381106 ID=MS.gene67838.t1.exon1;Parent=MS.gene67838.t1
chr2.4 CDS 57381106 57381395 57381106 ID=cds.MS.gene67838.t1;Parent=MS.gene67838.t1
chr2.4 exon 57381528 57381644 57381528 ID=MS.gene67838.t1.exon2;Parent=MS.gene67838.t1
chr2.4 CDS 57381528 57381644 57381528 ID=cds.MS.gene67838.t1;Parent=MS.gene67838.t1
chr2.4 exon 57382495 57382855 57382495 ID=MS.gene67838.t1.exon3;Parent=MS.gene67838.t1
chr2.4 CDS 57382495 57382855 57382495 ID=cds.MS.gene67838.t1;Parent=MS.gene67838.t1
Gene Sequence

>MS.gene67838

ATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTTTGTGATTTTTCAACACCCGTTGAACAACAACAGATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCTTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAACCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCCGCAACAATCAAACCTCAGATCAAGAAAAAGGTAATATATTTCTTTCTCTTATTTCATATATTTGAATTATAAATCAATTTTACGATGTCAAAATAATTGAAACACTTTTCCATAACTTAAATCTCATCGAACTAAAACCATTTTACCTTTCTTTTCTTACAGAAAAAAGAATGCCAAGATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCGTTGAAGGGTCTCCACATCCAAGGTTTTCATACATTCAAAACTATAACTTTAACATATGTTTCTTGTTTTTCACATTAAAAACAATAGTTTTCTTACCTAATACACACACCCACACCACATGTTTTAAATAGTGACAGTAAACTTTTGGATCCTTGCAATTTCATAGAATGGTAATTAAATTCAACATATGTGATCTTAGTCCCTGTAAAATTACTTTTTCTATTCATTGCAAATATTCCACCTTTGGTTTTTTGTCCCTGGTGTTTGTTATAGTTCCTAAGAAAAAATATTGAAATTGATCCATATAATATTCATTTTAATCCTTATTTTGATGGGCAAAAATTAAATATTCTACATATTGAGGATCCAATTTTAATATGAATAATATTTGCAGGGACTAAAGCAAAACAATAAAAACCAAAAGCCAAAGATGGTTTATTTCCAAAGACAAAATATATATATAGACACACACATATATGTCATGATGGGATCAAAAACATATGTAAATCTTAATTGTTTTTCAATTTTATCATTTAATTAACAATATTATTTTCATCATTCCTAAGTTTATATCTTTCACTTTAGATTTATGATAATTGATAAACAAACAATTGATACGGTCTTTCAAAAAAAAAAACAATTGATACGGTTAATTTGATATAGTTTTTTTTTTCTTCTTTTTTCGTTATAGTTTTTTTATTATCTATAAAAGAAATATGCCACTCATTGTGGGACGGAACCGATTGCATACCTTTATATAAAACATGTCCTATATATATGTGTAATGTGTGCGCGCGCAAATGGTTTTAGCTATTAAAATATTACAATTTAATGGTGCTTGATATAGAAAGAAATCTATGAATGATTTGTAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAGAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATGCTCAGGTTTTTTCCGATGAATCAAAAATCTCTTGTTCTGAAACAGACTTTATTGGAAACTCTAATGGTGATGATGACATTTTGATACCAAACATGACAACTCTGTCAAAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTTCCTTAG
Protein sequence

>MS.gene67838.t1

MTNTSVHETNQYPSVCDFSTPVEQQQIHQSNNNQVFVSKHVACIEITTAHFDRAYNHPSSPQHTQRSNQLPILETQTSSRVLPNTNPQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFVEGSPHPRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNAQVFSDESKISCSETDFIGNSNGDDDILIPNMTTLSKDLLMDFNHLNSGALFP