Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67953.t1 | RHN52993.1 | 56.8 | 88 | 36 | 2 | 7 | 93 | 13 | 99 | 1.90E-17 | 98.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67953.t1 | Q9SRH9 | 39.5 | 76 | 37 | 3 | 10 | 84 | 59 | 126 | 6.3e-06 | 51.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67953.t1 | G7KPU6 | 56.8 | 88 | 36 | 2 | 7 | 93 | 13 | 99 | 1.3e-17 | 98.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene67953.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67953.t1 | MTR_6g088340 | 56.818 | 88 | 36 | 2 | 7 | 93 | 13 | 99 | 1.83e-24 | 95.9 |
| MS.gene67953.t1 | MTR_6g092140 | 56.977 | 86 | 36 | 1 | 1 | 86 | 1 | 85 | 3.55e-24 | 95.1 |
| MS.gene67953.t1 | MTR_6g091830 | 56.977 | 86 | 36 | 1 | 1 | 86 | 1 | 85 | 3.81e-24 | 95.1 |
| MS.gene67953.t1 | MTR_3g006125 | 40.964 | 83 | 41 | 3 | 7 | 88 | 179 | 254 | 7.40e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGCATAAACATTTAATCTT+AGG | 0.203041 | 7.2:+64772137 | MS.gene67953:CDS |
| AATTTGTGTTGTGGTTAGTT+AGG | 0.304034 | 7.2:-64772333 | None:intergenic |
| ATTTGTGTTGTGGTTAGTTA+GGG | 0.399863 | 7.2:-64772332 | None:intergenic |
| TGTTCATCATACATTGGAAC+TGG | 0.420774 | 7.2:-64772197 | None:intergenic |
| TGTTCAAGAATTTAAAGAAA+AGG | 0.442218 | 7.2:+64772097 | MS.gene67953:CDS |
| AAGGTTTGTTTCGTGATGAT+AGG | 0.491333 | 7.2:+64771994 | MS.gene67953:CDS |
| GTTTGTTTCGTGATGATAGG+AGG | 0.521444 | 7.2:+64771997 | MS.gene67953:CDS |
| TTTGTTTCGTGATGATAGGA+GGG | 0.535881 | 7.2:+64771998 | MS.gene67953:CDS |
| CATGAACGATGTCGTGAGAC+TGG | 0.538407 | 7.2:+64772227 | MS.gene67953:CDS |
| CAACGTTGTTCATCATACAT+TGG | 0.551065 | 7.2:-64772203 | None:intergenic |
| ATTTAAAGAAAAGGTGAATG+AGG | 0.556980 | 7.2:+64772106 | MS.gene67953:CDS |
| AAGGTGAATGAGGTATTCAA+AGG | 0.561357 | 7.2:+64772116 | MS.gene67953:CDS |
| AGGTGAATGAGGTATTCAAA+GGG | 0.571151 | 7.2:+64772117 | MS.gene67953:CDS |
| CAAATCAACCTCAATGGCAA+AGG | 0.579217 | 7.2:+64771932 | None:intergenic |
| GATTCGATAATGCTTCATGT+AGG | 0.600602 | 7.2:-64772168 | None:intergenic |
| GATTCGACGATCATTCATGC+AGG | 0.620486 | 7.2:-64772063 | None:intergenic |
| TTGTTTCGTGATGATAGGAG+GGG | 0.624645 | 7.2:+64771999 | MS.gene67953:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTCAAGAATTTAAAGAAA+AGG | + | chr7.2:64772097-64772116 | MS.gene67953:CDS | 20.0% |
| !!! | TTTTCTTTAAATTCTTGAAC+AGG | - | chr7.2:64772098-64772117 | None:intergenic | 20.0% |
| ! | ATTTAAAGAAAAGGTGAATG+AGG | + | chr7.2:64772106-64772125 | MS.gene67953:CDS | 25.0% |
| GAATATGAAAAACCTGGAAT+TGG | + | chr7.2:64771954-64771973 | MS.gene67953:CDS | 30.0% | |
| GGGCATAAACATTTAATCTT+AGG | + | chr7.2:64772137-64772156 | MS.gene67953:CDS | 30.0% | |
| ! | AAAACCTGGAATTGGATTTT+TGG | + | chr7.2:64771962-64771981 | MS.gene67953:CDS | 30.0% |
| CAACGTTGTTCATCATACAT+TGG | - | chr7.2:64772206-64772225 | None:intergenic | 35.0% | |
| GATTCGATAATGCTTCATGT+AGG | - | chr7.2:64772171-64772190 | None:intergenic | 35.0% | |
| GCAAAGGAATATGAAAAACC+TGG | + | chr7.2:64771948-64771967 | MS.gene67953:CDS | 35.0% | |
| TGTTCATCATACATTGGAAC+TGG | - | chr7.2:64772200-64772219 | None:intergenic | 35.0% | |
| TTTGTTTCGTGATGATAGGA+GGG | + | chr7.2:64771998-64772017 | MS.gene67953:CDS | 35.0% | |
| ! | AAGGTGAATGAGGTATTCAA+AGG | + | chr7.2:64772116-64772135 | MS.gene67953:CDS | 35.0% |
| ! | AAGGTTTGTTTCGTGATGAT+AGG | + | chr7.2:64771994-64772013 | MS.gene67953:CDS | 35.0% |
| ! | AGGTGAATGAGGTATTCAAA+GGG | + | chr7.2:64772117-64772136 | MS.gene67953:CDS | 35.0% |
| !! | GGATTTTTGGAGGAAGTAAA+AGG | + | chr7.2:64771975-64771994 | MS.gene67953:CDS | 35.0% |
| GTTTGTTTCGTGATGATAGG+AGG | + | chr7.2:64771997-64772016 | MS.gene67953:CDS | 40.0% | |
| TCCTCCAAAAATCCAATTCC+AGG | - | chr7.2:64771969-64771988 | None:intergenic | 40.0% | |
| TTGTTTCGTGATGATAGGAG+GGG | + | chr7.2:64771999-64772018 | MS.gene67953:CDS | 40.0% | |
| !! | ACCTGGAATTGGATTTTTGG+AGG | + | chr7.2:64771965-64771984 | MS.gene67953:CDS | 40.0% |
| GATTCGACGATCATTCATGC+AGG | - | chr7.2:64772066-64772085 | None:intergenic | 45.0% | |
| CATGAACGATGTCGTGAGAC+TGG | + | chr7.2:64772227-64772246 | MS.gene67953:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 64771945 | 64772341 | 64771945 | ID=MS.gene67953 |
| chr7.2 | mRNA | 64771945 | 64772341 | 64771945 | ID=MS.gene67953.t1;Parent=MS.gene67953 |
| chr7.2 | exon | 64771945 | 64772248 | 64771945 | ID=MS.gene67953.t1.exon1;Parent=MS.gene67953.t1 |
| chr7.2 | CDS | 64771945 | 64772248 | 64771945 | ID=cds.MS.gene67953.t1;Parent=MS.gene67953.t1 |
| chr7.2 | exon | 64772322 | 64772341 | 64772322 | ID=MS.gene67953.t1.exon2;Parent=MS.gene67953.t1 |
| chr7.2 | CDS | 64772322 | 64772341 | 64772322 | ID=cds.MS.gene67953.t1;Parent=MS.gene67953.t1 |
| Gene Sequence |
| Protein sequence |
