Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67992.t1 | XP_024632961.1 | 86.5 | 311 | 18 | 3 | 1 | 287 | 1 | 311 | 3.40E-130 | 474.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67992.t1 | Q9SZL8 | 27.1 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 3.2e-20 | 100.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67992.t1 | A0A072TE13 | 80.5 | 303 | 36 | 3 | 1 | 287 | 1 | 296 | 1.0e-128 | 469.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene67992.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67992.t1 | MTR_0558s0020 | 86.469 | 303 | 18 | 3 | 1 | 287 | 1 | 296 | 0.0 | 529 |
| MS.gene67992.t1 | MTR_1g083640 | 84.965 | 286 | 19 | 3 | 1 | 262 | 1 | 286 | 5.42e-176 | 487 |
| MS.gene67992.t1 | MTR_8g446020 | 73.597 | 303 | 17 | 4 | 1 | 287 | 1 | 256 | 6.55e-151 | 422 |
| MS.gene67992.t1 | MTR_2g014100 | 85.714 | 245 | 18 | 3 | 1 | 228 | 1 | 245 | 1.08e-146 | 412 |
| MS.gene67992.t1 | MTR_6g090505 | 86.087 | 230 | 16 | 2 | 73 | 286 | 1 | 230 | 3.86e-141 | 402 |
| MS.gene67992.t1 | MTR_0337s0040 | 69.030 | 268 | 21 | 4 | 1 | 252 | 1 | 222 | 1.99e-119 | 342 |
| MS.gene67992.t1 | MTR_3g008240 | 80.476 | 210 | 18 | 3 | 1 | 194 | 1 | 203 | 1.56e-114 | 328 |
| MS.gene67992.t1 | MTR_4g040430 | 79.524 | 210 | 15 | 4 | 1 | 194 | 1 | 198 | 4.60e-111 | 319 |
| MS.gene67992.t1 | MTR_5g071590 | 73.333 | 180 | 27 | 3 | 1 | 159 | 1 | 180 | 2.04e-88 | 261 |
| MS.gene67992.t1 | MTR_0019s0290 | 78.947 | 152 | 16 | 2 | 90 | 225 | 9 | 160 | 1.16e-78 | 236 |
| MS.gene67992.t1 | MTR_1g055240 | 82.517 | 143 | 9 | 2 | 98 | 224 | 2 | 144 | 4.02e-78 | 234 |
| MS.gene67992.t1 | MTR_1281s0010 | 85.294 | 102 | 7 | 1 | 27 | 120 | 1 | 102 | 1.15e-55 | 176 |
| MS.gene67992.t1 | MTR_8g046060 | 88.636 | 88 | 9 | 1 | 1 | 87 | 91 | 178 | 1.11e-42 | 146 |
| MS.gene67992.t1 | MTR_4g008820 | 82.759 | 87 | 8 | 1 | 1 | 87 | 125 | 204 | 1.78e-38 | 143 |
| MS.gene67992.t1 | MTR_6g051425 | 30.417 | 240 | 154 | 4 | 48 | 280 | 22 | 255 | 1.45e-24 | 101 |
| MS.gene67992.t1 | MTR_1g070355 | 27.572 | 243 | 157 | 7 | 49 | 283 | 80 | 311 | 6.19e-16 | 78.2 |
| MS.gene67992.t1 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 251 | 286 | 14 | 49 | 1.14e-14 | 68.2 |
| MS.gene67992.t1 | MTR_1g013060 | 28.241 | 216 | 120 | 9 | 48 | 245 | 29 | 227 | 6.24e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67992.t1 | AT4G38180 | 27.059 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 5.19e-21 | 93.6 |
| MS.gene67992.t1 | AT5G28530 | 25.101 | 247 | 165 | 7 | 48 | 279 | 60 | 301 | 5.00e-14 | 72.8 |
| MS.gene67992.t1 | AT5G28530 | 25.101 | 247 | 165 | 7 | 48 | 279 | 60 | 301 | 5.37e-14 | 72.4 |
| MS.gene67992.t1 | AT5G28530 | 25.101 | 247 | 165 | 7 | 48 | 279 | 60 | 301 | 5.37e-14 | 72.4 |
| MS.gene67992.t1 | AT1G10240 | 23.504 | 234 | 175 | 4 | 57 | 287 | 62 | 294 | 1.60e-11 | 65.1 |
| MS.gene67992.t1 | AT1G10240 | 23.504 | 234 | 175 | 4 | 57 | 287 | 62 | 294 | 1.60e-11 | 65.1 |
Find 38 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTAGATTATTCTCTATT+TGG | 0.147857 | 6.4:+11311444 | MS.gene67992:CDS |
| ATAACACTGGATTCTCTATT+AGG | 0.309117 | 6.4:+11310854 | MS.gene67992:CDS |
| ACAAACTCAATAAAGTTGTT+AGG | 0.316138 | 6.4:-11310985 | None:intergenic |
| GGCGATATTGATTGTTTGTT+CGG | 0.357702 | 6.4:-11311494 | None:intergenic |
| CAAGAATTCTTTAGAAATGA+AGG | 0.372556 | 6.4:+11311336 | MS.gene67992:CDS |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 6.4:+11311203 | MS.gene67992:CDS |
| GTACAAAATACTAACCTAAT+GGG | 0.422907 | 6.4:-11311515 | None:intergenic |
| ATGTCTATAAGATATATAGA+TGG | 0.423693 | 6.4:+11311058 | MS.gene67992:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 6.4:+11311216 | MS.gene67992:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.4:+11311141 | MS.gene67992:CDS |
| AACAATCAATATCGCCCATT+AGG | 0.430510 | 6.4:+11311501 | MS.gene67992:CDS |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 6.4:-11310774 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 6.4:-11310775 | None:intergenic |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 6.4:+11311284 | MS.gene67992:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.465202 | 6.4:+11311171 | MS.gene67992:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 6.4:+11311204 | MS.gene67992:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 6.4:+11310937 | MS.gene67992:CDS |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 6.4:+11310773 | MS.gene67992:CDS |
| TGTCTTGTGCAGCATCAACT+TGG | 0.502977 | 6.4:-11311389 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 6.4:+11310948 | MS.gene67992:CDS |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 6.4:+11310841 | MS.gene67992:CDS |
| TGTCTATAAGATATATAGAT+GGG | 0.523615 | 6.4:+11311059 | MS.gene67992:CDS |
| TTTAAGTCCGAAAACCGAAA+AGG | 0.524077 | 6.4:-11311255 | None:intergenic |
| AAATGCCCTGGTTTATGTAA+TGG | 0.529222 | 6.4:-11311099 | None:intergenic |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 6.4:+11310903 | MS.gene67992:CDS |
| TAGACACTAAGCAGTTCAAA+AGG | 0.536783 | 6.4:-11311615 | None:intergenic |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 6.4:-11310773 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 6.4:+11310949 | MS.gene67992:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 6.4:+11311297 | MS.gene67992:CDS |
| CTCAAGTAACACAAATGCCC+TGG | 0.558368 | 6.4:-11311111 | None:intergenic |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 6.4:+11311290 | MS.gene67992:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.567659 | 6.4:+11311065 | MS.gene67992:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 6.4:+11311219 | MS.gene67992:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 6.4:+11311210 | MS.gene67992:CDS |
| TGTCATCCATTACATAAACC+AGG | 0.618164 | 6.4:+11311093 | MS.gene67992:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 6.4:+11311293 | MS.gene67992:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 6.4:+11311224 | MS.gene67992:CDS |
| GTCATCCATTACATAAACCA+GGG | 0.682651 | 6.4:+11311094 | MS.gene67992:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr6.4:11310903-11310922 | MS.gene67992:CDS | 20.0% |
| !! | ATGTCTATAAGATATATAGA+TGG | + | chr6.4:11311058-11311077 | MS.gene67992:CDS | 20.0% |
| !! | TGTCTATAAGATATATAGAT+GGG | + | chr6.4:11311059-11311078 | MS.gene67992:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | + | chr6.4:11310820-11310839 | MS.gene67992:CDS | 20.0% |
| ! | ACAAACTCAATAAAGTTGTT+AGG | - | chr6.4:11310988-11311007 | None:intergenic | 25.0% |
| ! | AGTACAAAATACTAACCTAA+TGG | - | chr6.4:11311519-11311538 | None:intergenic | 25.0% |
| ! | CAACTAGATTATTCTCTATT+TGG | + | chr6.4:11311444-11311463 | MS.gene67992:CDS | 25.0% |
| ! | GTACAAAATACTAACCTAAT+GGG | - | chr6.4:11311518-11311537 | None:intergenic | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | - | chr6.4:11311372-11311391 | None:intergenic | 25.0% |
| !! | CAAGAATTCTTTAGAAATGA+AGG | + | chr6.4:11311336-11311355 | MS.gene67992:CDS | 25.0% |
| !! | CATTATGTTGTATTTTGTTC+TGG | + | chr6.4:11311569-11311588 | MS.gene67992:intron | 25.0% |
| !!! | ACTTGGAATAATTTTGAAGT+TGG | + | chr6.4:11311023-11311042 | MS.gene67992:CDS | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | + | chr6.4:11311171-11311190 | MS.gene67992:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr6.4:11310949-11310968 | MS.gene67992:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | + | chr6.4:11311204-11311223 | MS.gene67992:CDS | 30.0% | |
| ATAACACTGGATTCTCTATT+AGG | + | chr6.4:11310854-11310873 | MS.gene67992:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | + | chr6.4:11310937-11310956 | MS.gene67992:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | + | chr6.4:11310841-11310860 | MS.gene67992:CDS | 30.0% | |
| GTGAAAGTGAAAGAAAAACT+TGG | + | chr6.4:11311006-11311025 | MS.gene67992:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr6.4:11310948-11310967 | MS.gene67992:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | chr6.4:11311065-11311084 | MS.gene67992:CDS | 30.0% | |
| TCCATGATTCTTTATTGCTA+GGG | - | chr6.4:11310777-11310796 | None:intergenic | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | + | chr6.4:11311284-11311303 | MS.gene67992:CDS | 30.0% | |
| TTCCATGATTCTTTATTGCT+AGG | - | chr6.4:11310778-11310797 | None:intergenic | 30.0% | |
| ! | TAGTATTTTGTACTTCTCTC+AGG | + | chr6.4:11311525-11311544 | MS.gene67992:intron | 30.0% |
| !!! | AAGACATAGCTAGCATTTTT+TGG | + | chr6.4:11311406-11311425 | MS.gene67992:CDS | 30.0% |
| !!! | AGACATAGCTAGCATTTTTT+GGG | + | chr6.4:11311407-11311426 | MS.gene67992:CDS | 30.0% |
| AAATGCCCTGGTTTATGTAA+TGG | - | chr6.4:11311102-11311121 | None:intergenic | 35.0% | |
| AACAATCAATATCGCCCATT+AGG | + | chr6.4:11311501-11311520 | MS.gene67992:CDS | 35.0% | |
| CCATGATTCTTTATTGCTAG+GGG | - | chr6.4:11310776-11310795 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr6.4:11311141-11311160 | MS.gene67992:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | chr6.4:11311203-11311222 | MS.gene67992:CDS | 35.0% | |
| GTCATCCATTACATAAACCA+GGG | + | chr6.4:11311094-11311113 | MS.gene67992:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | chr6.4:11311210-11311229 | MS.gene67992:CDS | 35.0% | |
| TGTCATCCATTACATAAACC+AGG | + | chr6.4:11311093-11311112 | MS.gene67992:CDS | 35.0% | |
| TTTAAGTCCGAAAACCGAAA+AGG | - | chr6.4:11311258-11311277 | None:intergenic | 35.0% | |
| ! | GAATCTTCCTTTTCGGTTTT+CGG | + | chr6.4:11311248-11311267 | MS.gene67992:CDS | 35.0% |
| ! | GGCGATATTGATTGTTTGTT+CGG | - | chr6.4:11311497-11311516 | None:intergenic | 35.0% |
| ! | TGTTGTATTTTGTTCTGGCA+TGG | + | chr6.4:11311574-11311593 | MS.gene67992:intron | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | + | chr6.4:11311293-11311312 | MS.gene67992:CDS | 40.0% | |
| CCCCTAGCAATAAAGAATCA+TGG | + | chr6.4:11310773-11310792 | MS.gene67992:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | + | chr6.4:11311290-11311309 | MS.gene67992:CDS | 40.0% | |
| TGTTCTGGCATGGATAGTAT+AGG | + | chr6.4:11311584-11311603 | MS.gene67992:intron | 40.0% | |
| !!! | GCTAGCATTTTTTGGGCATA+TGG | + | chr6.4:11311414-11311433 | MS.gene67992:CDS | 40.0% |
| CTCAAGTAACACAAATGCCC+TGG | - | chr6.4:11311114-11311133 | None:intergenic | 45.0% | |
| CTGGCATGGATAGTATAGGT+AGG | + | chr6.4:11311588-11311607 | MS.gene67992:intron | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr6.4:11311216-11311235 | MS.gene67992:CDS | 45.0% | |
| TGTCTTGTGCAGCATCAACT+TGG | - | chr6.4:11311392-11311411 | None:intergenic | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | + | chr6.4:11311241-11311260 | MS.gene67992:CDS | 45.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr6.4:11311219-11311238 | MS.gene67992:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | + | chr6.4:11311297-11311316 | MS.gene67992:CDS | 50.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr6.4:11311224-11311243 | MS.gene67992:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 11310664 | 11311622 | 11310664 | ID=MS.gene67992 |
| chr6.4 | mRNA | 11310664 | 11311622 | 11310664 | ID=MS.gene67992.t1;Parent=MS.gene67992 |
| chr6.4 | exon | 11310664 | 11311522 | 11310664 | ID=MS.gene67992.t1.exon1;Parent=MS.gene67992.t1 |
| chr6.4 | CDS | 11310664 | 11311522 | 11310664 | ID=cds.MS.gene67992.t1;Parent=MS.gene67992.t1 |
| chr6.4 | exon | 11311606 | 11311622 | 11311606 | ID=MS.gene67992.t1.exon2;Parent=MS.gene67992.t1 |
| chr6.4 | CDS | 11311606 | 11311622 | 11311606 | ID=cds.MS.gene67992.t1;Parent=MS.gene67992.t1 |
| Gene Sequence |
| Protein sequence |
