AlfalfaGEDB | MS.gene68190

M. sativa cultivar XinJiangDaYe / MS.gene68190

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68190.t1	XP_003603989.1	68	194	15	2	1	147	1	194	1.50E-57	232.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68190.t1	Q9LQZ7	33.0	206	79	4	1	147	1	206	7.1e-16	85.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68190.t1	G7J7R4	68.0	194	15	2	1	147	1	194	1.1e-57	232.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene68190.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68190.t1	MTR_3g117320	72.165	194	7	2	1	147	1	194	4.78e-89	259
MS.gene68190.t1	MTR_4g071200	43.005	193	56	5	1	146	1	186	1.53e-36	125
MS.gene68190.t1	MTR_1g023260	37.629	194	65	4	1	148	1	184	3.52e-26	98.2
MS.gene68190.t1	MTR_3g113070	40.260	154	65	4	1	138	1	143	6.64e-26	98.2
MS.gene68190.t1	MTR_1g023260	37.306	193	65	4	1	147	1	183	4.97e-25	97.1
MS.gene68190.t1	MTR_2g099010	66.667	48	16	0	1	48	1	48	6.98e-15	69.3
MS.gene68190.t1	MTR_2g099010	66.667	48	16	0	1	48	1	48	7.29e-15	69.3
MS.gene68190.t1	MTR_4g067320	64.583	48	17	0	1	48	1	48	1.94e-14	68.2
MS.gene68190.t1	MTR_4g067320	64.583	48	17	0	1	48	1	48	2.07e-14	68.2
MS.gene68190.t1	MTR_2g073370	34.711	121	34	1	1	76	1	121	2.31e-14	68.6
MS.gene68190.t1	MTR_1g023260	37.226	137	69	4	11	147	56	175	9.46e-14	66.6
MS.gene68190.t1	MTR_5g021580	53.191	47	22	0	1	47	1	47	1.90e-11	59.3
MS.gene68190.t1	MTR_5g021580	53.191	47	22	0	1	47	1	47	2.09e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68190.t1	AT1G75540	37.864	206	69	4	1	147	1	206	4.40e-30	111
MS.gene68190.t1	AT4G39070	38.000	200	61	6	1	137	1	200	1.26e-21	87.4
MS.gene68190.t1	AT4G10240	37.097	124	30	2	1	76	1	124	2.90e-15	68.9
MS.gene68190.t1	AT1G78600	35.897	117	30	1	1	72	1	117	9.47e-15	69.7
MS.gene68190.t1	AT1G06040	68.085	47	15	0	1	47	1	47	1.19e-14	67.8
MS.gene68190.t1	AT1G78600	35.897	117	30	1	1	72	1	117	1.20e-14	69.7
MS.gene68190.t1	AT2G31380	65.957	47	16	0	1	47	1	47	3.76e-14	67.4
MS.gene68190.t1	AT1G06040	68.085	47	15	0	1	47	1	47	4.15e-14	67.4
MS.gene68190.t1	AT4G38960	55.319	47	21	0	1	47	1	47	3.41e-12	61.2
MS.gene68190.t1	AT4G38960	55.319	47	21	0	1	47	1	47	3.41e-12	61.2
MS.gene68190.t1	AT2G21320	55.319	47	21	0	1	47	1	47	9.54e-12	60.1

Find 0 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTCATTTTTTTTTATAAAAA+CGG	-	57668:59679-59698	MS.gene68190:CDS	10.0%
!!!	ATGTGACTAATGTTTTAAAA+TGG	-	57668:59761-59780	MS.gene68190:CDS	20.0%
!!!	TTTTTTTATAAAAACGGTCG+CGG	-	57668:59685-59704	MS.gene68190:CDS	25.0%
	AACATACACAGAAACATAAC+AGG	-	57668:59911-59930	MS.gene68190:intron	30.0%
	ATTAAAGATCCAGATGCAAA+CGG	+	57668:59720-59739	None:intergenic	30.0%
	TAAACCTTACAGAAACCATT+AGG	+	57668:60207-60226	None:intergenic	30.0%
!	AGAAGAACTTGCATTTTCAT+TGG	+	57668:60061-60080	None:intergenic	30.0%
!	TTTTCATTGGAAACTGATCT+TGG	+	57668:60048-60067	None:intergenic	30.0%
	AAATGCAAGTTCTTCTTGCA+TGG	-	57668:60065-60084	MS.gene68190:intron	35.0%
	AAGTCTTCAAAACAGTAACC+AGG	+	57668:60165-60184	None:intergenic	35.0%
	ACTTGACAGATATCACAAAG+AGG	+	57668:59648-59667	None:intergenic	35.0%
	ATAGAATCGCTCTATCTTCT+TGG	+	57668:59855-59874	None:intergenic	35.0%
	CTTGACAGATATCACAAAGA+GGG	+	57668:59647-59666	None:intergenic	35.0%
	GAAGCTTTCAAGCAATTCTT+TGG	-	57668:59951-59970	MS.gene68190:intron	35.0%
	TACCTGATTGAAACAATACC+TGG	-	57668:60144-60163	MS.gene68190:CDS	35.0%
!	AACCAGGTATTGTTTCAATC+AGG	+	57668:60149-60168	None:intergenic	35.0%
!	TTTGATCTAGAGTGGTTTCT+TGG	+	57668:60012-60031	None:intergenic	35.0%
!!	TGTTTGCTTTTGATCTAGAG+TGG	+	57668:60020-60039	None:intergenic	35.0%
	ACCTTACAGAAACCATTAGG+TGG	+	57668:60204-60223	None:intergenic	40.0%
	ATCACAAAGAGGGTAGTCTT+TGG	+	57668:59637-59656	None:intergenic	40.0%
	CATAACAGGTTTCTTCTCTC+TGG	-	57668:59925-59944	MS.gene68190:intron	40.0%
	CCTTACAGAAACCATTAGGT+GGG	+	57668:60203-60222	None:intergenic	40.0%
	CTTTCTCACACACATCACAT+TGG	+	57668:59491-59510	None:intergenic	40.0%
	GAGAGAAAAACGAGTGTGTT+TGG	+	57668:59601-59620	None:intergenic	40.0%
	GATTCATCATCCACCAGTAT+TGG	-	57668:59979-59998	MS.gene68190:intron	40.0%
	GTGTATGTTCATTTGCTCCA+TGG	+	57668:59900-59919	None:intergenic	40.0%
	TATAAAAACGGTCGCGGTAA+AGG	-	57668:59691-59710	MS.gene68190:CDS	40.0%
	TGTTCATTTGCTCCATGGAT+TGG	+	57668:59895-59914	None:intergenic	40.0%
	TTCATCTGAAGGACAGAACA+TGG	+	57668:59523-59542	None:intergenic	40.0%
!	AATATCGTGCGCTTTTGTAC+AGG	-	57668:59807-59826	MS.gene68190:intron	40.0%
!	ATACTGGTGGATGATGAATC+TGG	+	57668:59979-59998	None:intergenic	40.0%
!	TACTGGTGGATGATGAATCT+GGG	+	57668:59978-59997	None:intergenic	40.0%
	CAAAGAGCAGCTTCATCTGA+AGG	+	57668:59534-59553	None:intergenic	45.0%
	CCCACCTAATGGTTTCTGTA+AGG	-	57668:60200-60219	MS.gene68190:CDS	45.0%
	GAATGTGACCTTCCAATCCA+TGG	-	57668:59880-59899	MS.gene68190:intron	45.0%
	GATGCTTCTTTCCCACCTAA+TGG	-	57668:60189-60208	MS.gene68190:CDS	45.0%
	GGATGGTATGATCACATCCA+TGG	+	57668:59557-59576	None:intergenic	45.0%
	TCTTGGTTCAGAGCCAATAC+TGG	+	57668:59995-60014	None:intergenic	45.0%
	TGATGTGTGTGAGAAAGCTG+AGG	-	57668:59494-59513	MS.gene68190:CDS	45.0%
	TTGCATGGCTGAAGACAACA+TGG	-	57668:60080-60099	MS.gene68190:intron	45.0%
!	TAAAGGTTGCCGTTTGCATC+TGG	-	57668:59708-59727	MS.gene68190:CDS	45.0%
!!	CATTTGCTCCATGGATTGGA+AGG	+	57668:59891-59910	None:intergenic	45.0%
!!	TGGTTGCAAGCTTGTTAGCA+CGG	+	57668:59581-59600	None:intergenic	45.0%
	GACAACATGGCTAGTGACAC+TGG	-	57668:60093-60112	MS.gene68190:CDS	50.0%
	GATGAAGCTGCTCTTTGCCA+TGG	-	57668:59537-59556	MS.gene68190:CDS	50.0%
	TGGTTCAGAGCCAATACTGG+TGG	+	57668:59992-60011	None:intergenic	50.0%
!!	AAGCTTGTTAGCACGGTGGA+TGG	+	57668:59574-59593	None:intergenic	50.0%
!!	GAGGGTAGTCTTTGGAGTTG+AGG	+	57668:59629-59648	None:intergenic	50.0%
!!	GGTAGTCTTTGGAGTTGAGG+TGG	+	57668:59626-59645	None:intergenic	50.0%
!!	TTGCAAGCTTGTTAGCACGG+TGG	+	57668:59578-59597	None:intergenic	50.0%
!	CGTGCGCTTTTGTACAGGAG+AGG	-	57668:59812-59831	MS.gene68190:intron	55.0%
!	GCTTTTGTACAGGAGAGGCG+TGG	-	57668:59817-59836	MS.gene68190:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
57668	gene	59480	60227	59480	ID=MS.gene68190
57668	mRNA	59480	60227	59480	ID=MS.gene68190.t1;Parent=MS.gene68190
57668	exon	60087	60227	60087	ID=MS.gene68190.t1.exon1;Parent=MS.gene68190.t1
57668	CDS	60087	60227	60087	ID=cds.MS.gene68190.t1;Parent=MS.gene68190.t1
57668	exon	59480	59785	59480	ID=MS.gene68190.t1.exon2;Parent=MS.gene68190.t1
57668	CDS	59480	59785	59480	ID=cds.MS.gene68190.t1;Parent=MS.gene68190.t1

Gene Sequence

>MS.gene68190

ATGAAGATCCAATGTGATGTGTGTGAGAAAGCTGAGGCTTCCATGTTCTGTCCTTCAGATGAAGCTGCTCTTTGCCATGGATGTGATCATACCATCCACCGTGCTAACAAGCTTGCAACCAAACACACTCGTTTTTCTCTCGTCCACCTCAACTCCAAAGACTACCCTCTTTGTGATATCTGTCAAGTATGAATACACTGTCATTTTTTTTTATAAAAACGGTCGCGGTAAAGGTTGCCGTTTGCATCTGGATCTTTAATATTTTAAAGAATCTCATTCAGATGTGACTAATGTTTTAAAATGGTCATTAATGTCACTTTAATTAATAATATCGTGCGCTTTTGTACAGGAGAGGCGTGGATATCTATTTTGCCAAGAAGATAGAGCGATTCTATGCAGAGAATGTGACCTTCCAATCCATGGAGCAAATGAACATACACAGAAACATAACAGGTTTCTTCTCTCTGGTGTGAAGCTTTCAAGCAATTCTTTGGACCCAGATTCATCATCCACCAGTATTGGCTCTGAACCAAGAAACCACTCTAGATCAAAAGCAAACATAATACCAAGATCAGTTTCCAATGAAAATGCAAGTTCTTCTTGCATGGCTGAAGACAACATGGCTAGTGACACTGGTTCTGTTTCAACAAGCAGCATTTCTGAATACCTGATTGAAACAATACCTGGTTACTGTTTTGAAGACTTTCTTGATGCTTCTTTCCCACCTAATGGTTTCTGTAAGGTTTAA

Protein sequence

>MS.gene68190.t1

MKIQCDVCEKAEASMFCPSDEAALCHGCDHTIHRANKLATKHTRFSLKHNRFLLSGVKLSSNSLDPDSSSTSIGSEPRNHSRSKANIIPRSVSNENASSSCMAEDNMASDTGSVSTSSISEYLIETIPGYCFEDFLDASFPPNGFCKV