AlfalfaGEDB | MS.gene68270

M. sativa cultivar XinJiangDaYe / MS.gene68270

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68270.t1	AES87959.1	83.8	117	18	1	1	116	1	117	3.00E-13	84.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68270.t1	G7JTJ2	83.8	117	18	1	1	116	1	117	2.2e-13	84.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene68270.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68270.t1	MTR_4g039380	83.761	117	18	1	1	116	1	117	1.64e-61	184
MS.gene68270.t1	MTR_4g039340	74.809	131	18	2	1	116	1	131	8.09e-57	172
MS.gene68270.t1	MTR_4g039640	77.778	117	25	1	1	116	1	117	4.28e-48	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTCAAATGGTTGCACATG+TGG	0.522527	4.3:+61431017	None:intergenic
AATGGTTGCACATGTGGAGG+TGG	0.555990	4.3:+61431023	None:intergenic
ACTCGATTCTAATATAAAAT+CGG	0.323743	4.3:+61431196	None:intergenic
ACTGCTAAAGCTTCACGAGA+TGG	0.614779	4.3:+61431242	None:intergenic
AGGTCGAAAGCTCGAACAGG+AGG	0.636300	4.3:+61430987	None:intergenic
ATATTAGAATCGAGTAAACA+AGG	0.624706	4.3:-61431188	MS.gene68270:CDS
CGAAAGCTCGAACAGGAGGT+TGG	0.564077	4.3:+61430991	None:intergenic
CTCATTCATCTGGTGTATAA+GGG	0.375685	4.3:+61430958	None:intergenic
CTCGATTCTAATATAAAATC+GGG	0.421980	4.3:+61431197	None:intergenic
CTGGTGTATAAGGGAGATTG+AGG	0.611261	4.3:+61430967	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCAAATGGTTGCACATG+TGG	+	chr4.3:61431232-61431251	None:intergenic	40.0%
	ACTGCTAAAGCTTCACGAGA+TGG	+	chr4.3:61431007-61431026	None:intergenic	45.0%
	CTGGTGTATAAGGGAGATTG+AGG	+	chr4.3:61431282-61431301	None:intergenic	45.0%
	TCAAATGGTTGCACATGTGG+AGG	+	chr4.3:61431229-61431248	None:intergenic	45.0%
	AATGGTTGCACATGTGGAGG+TGG	+	chr4.3:61431226-61431245	None:intergenic	50.0%
	TTGAGGTCGAAAGCTCGAAC+AGG	+	chr4.3:61431265-61431284	None:intergenic	50.0%
	AGGTCGAAAGCTCGAACAGG+AGG	+	chr4.3:61431262-61431281	None:intergenic	55.0%
	CGAAAGCTCGAACAGGAGGT+TGG	+	chr4.3:61431258-61431277	None:intergenic	55.0%
!	ATATTAGAATCGAGTAAACA+AGG	-	chr4.3:61431058-61431077	MS.gene68270:CDS	25.0%
!	CTCGATTCTAATATAAAATC+GGG	+	chr4.3:61431052-61431071	None:intergenic	25.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	61430959	61431309	61430959	ID=MS.gene68270
chr4.3	mRNA	61430959	61431309	61430959	ID=MS.gene68270.t1;Parent=MS.gene68270
chr4.3	exon	61430959	61431309	61430959	ID=MS.gene68270.t1.exon1;Parent=MS.gene68270.t1
chr4.3	CDS	61430959	61431309	61430959	ID=cds.MS.gene68270.t1;Parent=MS.gene68270.t1

Gene Sequence	>MS.gene68270 ATGTCAAAGCTAGAATTCTTATGCAGTGAATGTGACACAATGTTTCCATCTCGTGAAGCTTTAGCAGTGCATGTTATCGAAGAACATCATCCCGATTTTATATTAGAATCGAGTAAACAAGGTCCACCACCAAATAACCAGCCCGAACCAGAAAATGCTCAGCCTCAACCACAAAATGTTGAACATCAACCACAAAATGCACTAGCTCCACCACAAAATGTTGAACAACAACCACAAAATGCACCAGCTCCACCACAAAATGCACCACCTCCACATGTGCAACCATTTGACTTGAACCAACCTCCTGTTCGAGCTTTCGACCTCAATCTCCCTTATACACCAGATGAATGA
Protein sequence	>MS.gene68270.t1 MSKLEFLCSECDTMFPSREALAVHVIEEHHPDFILESSKQGPPPNNQPEPENAQPQPQNVEHQPQNALAPPQNVEQQPQNAPAPPQNAPPPHVQPFDLNQPPVRAFDLNLPYTPDE