AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68336

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68336.t1 MTR_5g035040 80.464 302 35 1 33 334 61 338 2.13e-176 494
MS.gene68336.t1 MTR_5g035060 60.153 261 55 6 123 334 1 261 3.39e-86 262
MS.gene68336.t1 MTR_7g033775 73.529 68 18 0 39 106 81 148 2.89e-25 100
MS.gene68336.t1 MTR_5g035050 88.000 50 6 0 285 334 29 78 3.58e-23 93.2
MS.gene68336.t1 MTR_4g105200 56.716 67 29 0 20 86 52 118 2.28e-18 82.4
MS.gene68336.t1 MTR_1g090250 36.697 109 67 1 2 110 28 134 2.21e-14 70.1
MS.gene68336.t1 MTR_3g109190 36.275 102 64 1 6 106 32 133 1.37e-13 68.2
MS.gene68336.t1 MTR_6g005040 41.026 78 46 0 26 103 80 157 1.18e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68336.t1 AT1G24040 57.653 196 77 1 145 334 80 275 1.25e-74 233
MS.gene68336.t1 AT1G24040 57.653 196 77 1 145 334 80 275 1.25e-74 233
MS.gene68336.t1 AT1G24040 57.653 196 77 1 145 334 80 275 1.25e-74 233
MS.gene68336.t1 AT3G01570 45.833 96 51 1 20 115 48 142 1.69e-19 85.1
MS.gene68336.t1 AT5G40420 53.521 71 33 0 36 106 81 151 1.15e-15 75.1
MS.gene68336.t1 AT3G27660 45.783 83 44 1 33 115 76 157 8.26e-15 72.4
MS.gene68336.t1 AT3G27660 45.783 83 44 1 33 115 76 157 8.26e-15 72.4
MS.gene68336.t1 AT5G51210 46.269 67 36 0 20 86 38 104 2.43e-14 69.7
MS.gene68336.t1 AT4G25140 44.444 54 30 0 32 85 66 119 8.07e-11 60.5

Find 81 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAAATCCGGTTCAATTT+TGG 0.113243 5.2:-25577109 MS.gene68336:CDS
CTTGCTGGACTCTCCCTTCT+TGG 0.217986 5.2:-25577540 MS.gene68336:CDS
CCTGTAGAGGCCCTTGATCC+TGG 0.232255 5.2:+25577352 None:intergenic
ACATGTTGATATAACATTTC+GGG 0.273357 5.2:+25577010 None:intergenic
AACCAAAATTGAACCGGATT+TGG 0.284192 5.2:+25577107 None:intergenic
TTGAACCGGATTTGGTGTTT+TGG 0.312879 5.2:+25577115 None:intergenic
AACATGTTGATATAACATTT+CGG 0.331289 5.2:+25577009 None:intergenic
GTTCCAGCCACCATTGTCAT+TGG 0.345015 5.2:-25577459 MS.gene68336:CDS
TAATCAAATATTGTTTCATA+AGG 0.361459 5.2:+25576980 None:intergenic
CCGCATGTGGCGACACTGAT+TGG 0.372849 5.2:-25576940 MS.gene68336:CDS
TCTTGGAACACTCACTGGAC+TGG 0.377988 5.2:-25577523 MS.gene68336:CDS
GAGGACTCGACGAGTGGTTT+TGG 0.383378 5.2:+25577188 None:intergenic
CTTGGAACACTCACTGGACT+GGG 0.386557 5.2:-25577522 MS.gene68336:CDS
TGGCACTGTCCTTGTTCTCA+TGG 0.386698 5.2:-25577392 MS.gene68336:CDS
GGAAGGATTCGAGGTGTTGC+AGG 0.387564 5.2:+25577145 None:intergenic
GGTGTACTGCTTGCACTTGC+TGG 0.398295 5.2:-25577555 MS.gene68336:CDS
GTGTTGCAGGTTGGTGAGTT+CGG 0.404578 5.2:+25577158 None:intergenic
ATGGTGGCTGGAACAAGAAC+AGG 0.405324 5.2:+25577468 None:intergenic
TACTCGAACCAAAATTGAAC+CGG 0.409292 5.2:+25577101 None:intergenic
TATGCGAGTAAACAGACTAT+TGG 0.414219 5.2:+25577057 None:intergenic
CTGTAGAGGCCCTTGATCCT+GGG 0.419299 5.2:+25577353 None:intergenic
CCTTAGGCCCTCAAAATCAT+TGG 0.420597 5.2:-25577221 MS.gene68336:CDS
TCCCTTCTTGGAACACTCAC+TGG 0.449866 5.2:-25577528 MS.gene68336:CDS
ATGCGAGTAAACAGACTATT+GGG 0.451833 5.2:+25577058 None:intergenic
ATTCTTTCACTCTCCTCCTT+AGG 0.453455 5.2:-25577237 MS.gene68336:CDS
GGAAGTAGAGGAAGGATTCG+AGG 0.455777 5.2:+25577136 None:intergenic
ATAAGTGTAAGGCGAATCCT+TGG 0.455782 5.2:+25576810 None:intergenic
CGCATGTGGCGACACTGATT+GGG 0.458513 5.2:-25576939 MS.gene68336:CDS
ATTCCCTTCAGGAGGGGCAT+TGG 0.469054 5.2:-25575914 MS.gene68336:intron
TACTTCCAAAACACCAAATC+CGG 0.469207 5.2:-25577120 MS.gene68336:CDS
CAAGTGAGGAACTGATTTCT+CGG 0.471299 5.2:-25575874 MS.gene68336:CDS
CCGTGGATAAATCTCTTAGA+AGG 0.481490 5.2:-25576780 MS.gene68336:intron
GGCACTGTCCTTGTTCTCAT+GGG 0.495063 5.2:-25577391 MS.gene68336:CDS
TTCGCCTTACACTTATAGCT+TGG 0.495890 5.2:-25576803 MS.gene68336:CDS
CCAGGATCAAGGGCCTCTAC+AGG 0.506135 5.2:-25577352 MS.gene68336:CDS
TCCAGTGAGTGTTCCAAGAA+GGG 0.516170 5.2:+25577527 None:intergenic
AATTACATACCCCAGGATCA+AGG 0.516525 5.2:-25577363 MS.gene68336:CDS
TTTCTTCAGTTCTATTACCA+TGG 0.524414 5.2:-25577265 MS.gene68336:CDS
GTCCAGTGAGTGTTCCAAGA+AGG 0.526230 5.2:+25577526 None:intergenic
TGCTTCACCCACTCCTCCCA+AGG 0.526357 5.2:-25576827 MS.gene68336:CDS
GGCTGAAGAGGATGAAAAGT+GGG 0.530470 5.2:+25577490 None:intergenic
AAGCCAATGACAATGGTGGC+TGG 0.533271 5.2:+25577456 None:intergenic
GAACAAGAACAGGGCTGAAG+AGG 0.534164 5.2:+25577478 None:intergenic
GGCGAATCCTTGGGAGGAGT+GGG 0.537228 5.2:+25576820 None:intergenic
CAACTTAATAACGATGTTGA+GGG 0.540781 5.2:+25577661 None:intergenic
ACGGCCAAGCTATAAGTGTA+AGG 0.541399 5.2:+25576799 None:intergenic
TTACACTTATAGCTTGGCCG+TGG 0.548191 5.2:-25576797 MS.gene68336:CDS
TCTACAGGAGCAACTCGAGT+TGG 0.549387 5.2:-25577337 MS.gene68336:CDS
AGGCGAATCCTTGGGAGGAG+TGG 0.550564 5.2:+25576819 None:intergenic
CCTTCAGGAGGGGCATTGGA+CGG 0.564318 5.2:-25575910 MS.gene68336:intron
AACTCGAGTTGCTCCTGTAG+AGG 0.565489 5.2:+25577339 None:intergenic
TAAGTGTAAGGCGAATCCTT+GGG 0.574212 5.2:+25576811 None:intergenic
TGGTGAGTTCGGAGTTTGTG+AGG 0.578381 5.2:+25577169 None:intergenic
GTTTGTGAGGACTCGACGAG+TGG 0.581088 5.2:+25577182 None:intergenic
TGTAGAGGCCCTTGATCCTG+GGG 0.582357 5.2:+25577354 None:intergenic
CCAACTTAATAACGATGTTG+AGG 0.588646 5.2:+25577660 None:intergenic
ATTACATACCCCAGGATCAA+GGG 0.590997 5.2:-25577362 MS.gene68336:CDS
GAGTGAAAGAATTGCTGCCA+TGG 0.598782 5.2:+25577248 None:intergenic
AACAATATTTGATTAAACGA+AGG 0.599669 5.2:-25576972 MS.gene68336:CDS
TGGAACACTCACTGGACTGG+GGG 0.600169 5.2:-25577520 MS.gene68336:CDS
GGTGAAGAAATGCAGTTGGC+TGG 0.602521 5.2:-25576895 MS.gene68336:CDS
GCTTATGGTGGAGTGGTGGT+CGG 0.603978 5.2:-25577307 MS.gene68336:CDS
GGATTCGAGGTGTTGCAGGT+TGG 0.605073 5.2:+25577149 None:intergenic
ATTCATGACCCATGAGAACA+AGG 0.605807 5.2:+25577383 None:intergenic
GGTCATGAATTACATACCCC+AGG 0.611661 5.2:-25577370 MS.gene68336:CDS
GGGCTGAAGAGGATGAAAAG+TGG 0.618573 5.2:+25577489 None:intergenic
AACAGCTTATGGTGGAGTGG+TGG 0.622508 5.2:-25577311 MS.gene68336:CDS
GACAGCAAAGCCAATGACAA+TGG 0.626525 5.2:+25577449 None:intergenic
CCTTCTAAGAGATTTATCCA+CGG 0.626878 5.2:+25576780 None:intergenic
CCAATCAGTGTCGCCACATG+CGG 0.627529 5.2:+25576940 None:intergenic
AAGGACGTTGATGCCGCATG+TGG 0.632685 5.2:-25576953 MS.gene68336:CDS
TGGTGGAGTGGTGGTCGGAG+TGG 0.638660 5.2:-25577302 MS.gene68336:CDS
TTGGAACACTCACTGGACTG+GGG 0.646592 5.2:-25577521 MS.gene68336:CDS
ACGATGTTGAGGGGTAACAC+TGG 0.650096 5.2:+25577671 None:intergenic
TGATGGTGAAGAAATGCAGT+TGG 0.661675 5.2:-25576899 MS.gene68336:CDS
GTGTAAGGCGAATCCTTGGG+AGG 0.670081 5.2:+25576814 None:intergenic
AACTTAATAACGATGTTGAG+GGG 0.672830 5.2:+25577662 None:intergenic
AGCAAAGCCAATGACAATGG+TGG 0.674104 5.2:+25577452 None:intergenic
TGGTGGCTGGAACAAGAACA+GGG 0.708517 5.2:+25577469 None:intergenic
CTGGTATCGTAACAGCGTGG+TGG 0.712087 5.2:+25577690 None:intergenic
ACACTGGTATCGTAACAGCG+TGG 0.804350 5.2:+25577687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATATTGTTTCATA+AGG + chr5.2:25576494-25576513 None:intergenic 15.0%
!! AACAATATTTGATTAAACGA+AGG - chr5.2:25576499-25576518 MS.gene68336:intron 20.0%
!! AACATGTTGATATAACATTT+CGG + chr5.2:25576465-25576484 None:intergenic 20.0%
!! AGCTTAATTATTTAAATTGC+AGG + chr5.2:25577384-25577403 None:intergenic 20.0%
!! TTTCGATTATTCAAAGATTA+AGG - chr5.2:25576872-25576891 MS.gene68336:CDS 20.0%
!!! GAGATTTGTTTTGATAAAAA+TGG - chr5.2:25576606-25576625 MS.gene68336:intron 20.0%
! AATAAGCTAAAATCAGCTTA+TGG + chr5.2:25577001-25577020 None:intergenic 25.0%
! ACATGTTGATATAACATTTC+GGG + chr5.2:25576464-25576483 None:intergenic 25.0%
! AGATTATGTAGGATATTTCT+TGG - chr5.2:25577249-25577268 MS.gene68336:CDS 25.0%
! ATAAAGAACTAATTTGACCA+AGG - chr5.2:25576788-25576807 MS.gene68336:CDS 25.0%
! GATTATGTAGGATATTTCTT+GGG - chr5.2:25577250-25577269 MS.gene68336:CDS 25.0%
! GGTTACTTAAATCTTAAACT+TGG + chr5.2:25577408-25577427 None:intergenic 25.0%
! TAAAGAACTAATTTGACCAA+GGG - chr5.2:25576789-25576808 MS.gene68336:CDS 25.0%
! TATTCTTGGTCTAATTTGAT+TGG - chr5.2:25576818-25576837 MS.gene68336:CDS 25.0%
! TCAAAAATGTTTCAGTTTGT+AGG + chr5.2:25576938-25576957 None:intergenic 25.0%
! TGTTTGAAAATGTTAGCTAT+GGG + chr5.2:25577456-25577475 None:intergenic 25.0%
! TTCACATATAGATACACTTT+GGG + chr5.2:25577155-25577174 None:intergenic 25.0%
! TTTCACATATAGATACACTT+TGG + chr5.2:25577156-25577175 None:intergenic 25.0%
!! TTGGTCAAATTAGTTCTTTA+TGG + chr5.2:25576789-25576808 None:intergenic 25.0%
AAATGCAAATTTCAGCAACT+TGG + chr5.2:25576736-25576755 None:intergenic 30.0%
AACTTAATAACGATGTTGAG+GGG + chr5.2:25575812-25575831 None:intergenic 30.0%
ATAATAGCAACGAGAAAATG+TGG + chr5.2:25577085-25577104 None:intergenic 30.0%
ATATTTAATTCCCTTCAGGA+GGG - chr5.2:25577550-25577569 MS.gene68336:CDS 30.0%
CAACTTAATAACGATGTTGA+GGG + chr5.2:25575813-25575832 None:intergenic 30.0%
GATTAAGGCTGTATTTAGAT+TGG - chr5.2:25576887-25576906 MS.gene68336:CDS 30.0%
GTGTTTGAAAATGTTAGCTA+TGG + chr5.2:25577457-25577476 None:intergenic 30.0%
TCTGATATTTAATTCCCTTC+AGG - chr5.2:25577546-25577565 MS.gene68336:CDS 30.0%
TGCTGAAATTTGCATTTCTT+TGG - chr5.2:25576739-25576758 MS.gene68336:intron 30.0%
TTTCTTCAGTTCTATTACCA+TGG - chr5.2:25576206-25576225 MS.gene68336:intron 30.0%
! AGCTAACATTTTCAAACACA+TGG - chr5.2:25577458-25577477 MS.gene68336:CDS 30.0%
! ATGTTGAACCAATGATTTTG+AGG + chr5.2:25576261-25576280 None:intergenic 30.0%
! GATATTTCTTGGGTGAATTT+TGG - chr5.2:25577260-25577279 MS.gene68336:CDS 30.0%
! GCTTTTGTATACATGTTAAG+TGG + chr5.2:25577674-25577693 None:intergenic 30.0%
! TGTTGAACCAATGATTTTGA+GGG + chr5.2:25576260-25576279 None:intergenic 30.0%
!! TCATCAGTAGCAATTTTAGT+TGG - chr5.2:25577353-25577372 MS.gene68336:CDS 30.0%
!!! CATCAGTAGCAATTTTAGTT+GGG - chr5.2:25577354-25577373 MS.gene68336:CDS 30.0%
AAAGCTAGAACTACTAGATG+CGG - chr5.2:25577173-25577192 MS.gene68336:CDS 35.0%
AACCAAAATTGAACCGGATT+TGG + chr5.2:25576367-25576386 None:intergenic 35.0%
ACCAAGAATATGTATTCCCT+TGG + chr5.2:25576808-25576827 None:intergenic 35.0%
ACCAAGGGAATACATATTCT+TGG - chr5.2:25576804-25576823 MS.gene68336:CDS 35.0%
ACTCTTCTGCTAGATTATGT+AGG - chr5.2:25577238-25577257 MS.gene68336:CDS 35.0%
ATGCGAGTAAACAGACTATT+GGG + chr5.2:25576416-25576435 None:intergenic 35.0%
ATTGGCTATATGCATATCCA+GGG - chr5.2:25576836-25576855 MS.gene68336:CDS 35.0%
CCAACTTAATAACGATGTTG+AGG + chr5.2:25575814-25575833 None:intergenic 35.0%
CCTCAACATCGTTATTAAGT+TGG - chr5.2:25575811-25575830 MS.gene68336:CDS 35.0%
GATATTTAATTCCCTTCAGG+AGG - chr5.2:25577549-25577568 MS.gene68336:CDS 35.0%
TACTCGAACCAAAATTGAAC+CGG + chr5.2:25576373-25576392 None:intergenic 35.0%
TACTTCCAAAACACCAAATC+CGG - chr5.2:25576351-25576370 MS.gene68336:intron 35.0%
TATGCGAGTAAACAGACTAT+TGG + chr5.2:25576417-25576436 None:intergenic 35.0%
TATTTAATTCCCTTCAGGAG+GGG - chr5.2:25577551-25577570 MS.gene68336:CDS 35.0%
TGTGTTCATAAACTATCCCT+GGG + chr5.2:25577034-25577053 None:intergenic 35.0%
TTGTGTTCATAAACTATCCC+TGG + chr5.2:25577035-25577054 None:intergenic 35.0%
! CCTTCTAAGAGATTTATCCA+CGG + chr5.2:25576694-25576713 None:intergenic 35.0%
! TCGCGACGAGAATATATTTT+CGG + chr5.2:25577429-25577448 None:intergenic 35.0%
! TTTTATAGAGGAGTTGCTGA+TGG - chr5.2:25576555-25576574 MS.gene68336:intron 35.0%
!! ACACTGATTGGGTTTTATAG+AGG - chr5.2:25576543-25576562 MS.gene68336:intron 35.0%
!! CAACATCGTTATTAAGTTGG+TGG - chr5.2:25575814-25575833 MS.gene68336:CDS 35.0%
!! CATTGGACGGCATATTTTAA+AGG - chr5.2:25577574-25577593 MS.gene68336:intron 35.0%
!! GCATATTTTAAAGGCAAGTG+AGG - chr5.2:25577583-25577602 MS.gene68336:intron 35.0%
!!! ATTTGGTGTTTTGGAAGTAG+AGG + chr5.2:25576350-25576369 None:intergenic 35.0%
AAGCTAGAACTACTAGATGC+GGG - chr5.2:25577174-25577193 MS.gene68336:CDS 40.0%
AATTACATACCCCAGGATCA+AGG - chr5.2:25576108-25576127 MS.gene68336:intron 40.0%
ACTACTAGATGCGGGTAAAA+TGG - chr5.2:25577182-25577201 MS.gene68336:CDS 40.0%
ATAAGTGTAAGGCGAATCCT+TGG + chr5.2:25576664-25576683 None:intergenic 40.0%
ATTACATACCCCAGGATCAA+GGG - chr5.2:25576109-25576128 MS.gene68336:intron 40.0%
ATTCATGACCCATGAGAACA+AGG + chr5.2:25576091-25576110 None:intergenic 40.0%
ATTCTTTCACTCTCCTCCTT+AGG - chr5.2:25576234-25576253 MS.gene68336:intron 40.0%
CCGTGGATAAATCTCTTAGA+AGG - chr5.2:25576691-25576710 MS.gene68336:intron 40.0%
CTTATTCCAACAAACTCCCA+GGG - chr5.2:25577015-25577034 MS.gene68336:CDS 40.0%
GTTGGAAAAACAGCTTATGG+TGG - chr5.2:25576152-25576171 MS.gene68336:intron 40.0%
TAAGTGTAAGGCGAATCCTT+GGG + chr5.2:25576663-25576682 None:intergenic 40.0%
TTCGCCTTACACTTATAGCT+TGG - chr5.2:25576668-25576687 MS.gene68336:intron 40.0%
TTGGCTATATGCATATCCAG+GGG - chr5.2:25576837-25576856 MS.gene68336:CDS 40.0%
! AAAAACAGCTTATGGTGGAG+TGG - chr5.2:25576157-25576176 MS.gene68336:intron 40.0%
! CAAGTGAGGAACTGATTTCT+CGG - chr5.2:25577597-25577616 MS.gene68336:intron 40.0%
! CACCAAATCCGGTTCAATTT+TGG - chr5.2:25576362-25576381 MS.gene68336:intron 40.0%
! CGAGTTGGAAAAACAGCTTA+TGG - chr5.2:25576149-25576168 MS.gene68336:intron 40.0%
! GATTGGCTATATGCATATCC+AGG - chr5.2:25576835-25576854 MS.gene68336:CDS 40.0%
! TGATGGTGAAGAAATGCAGT+TGG - chr5.2:25576572-25576591 MS.gene68336:intron 40.0%
!!! TTGAACCGGATTTGGTGTTT+TGG + chr5.2:25576359-25576378 None:intergenic 40.0%
AACTATCCCTGGGAGTTTGT+TGG + chr5.2:25577024-25577043 None:intergenic 45.0%
ACGGCCAAGCTATAAGTGTA+AGG + chr5.2:25576675-25576694 None:intergenic 45.0%
AGCAAAGCCAATGACAATGG+TGG + chr5.2:25576022-25576041 None:intergenic 45.0%
CAAGTGCTTGCCGTAATTAC+CGG - chr5.2:25575880-25575899 MS.gene68336:CDS 45.0%
CCTTAGGCCCTCAAAATCAT+TGG - chr5.2:25576250-25576269 MS.gene68336:intron 45.0%
GACAGCAAAGCCAATGACAA+TGG + chr5.2:25576025-25576044 None:intergenic 45.0%
GAGTGAAAGAATTGCTGCCA+TGG + chr5.2:25576226-25576245 None:intergenic 45.0%
GCAAGCACTTGAGAAGTTGA+TGG + chr5.2:25575871-25575890 None:intergenic 45.0%
GCTTATTCCAACAAACTCCC+AGG - chr5.2:25577014-25577033 MS.gene68336:CDS 45.0%
GGCTGAAGAGGATGAAAAGT+GGG + chr5.2:25575984-25576003 None:intergenic 45.0%
GGTCATGAATTACATACCCC+AGG - chr5.2:25576101-25576120 MS.gene68336:intron 45.0%
TACAGGAAGGCCGGTAATTA+CGG + chr5.2:25575893-25575912 None:intergenic 45.0%
TCCAGTGAGTGTTCCAAGAA+GGG + chr5.2:25575947-25575966 None:intergenic 45.0%
TTACACTTATAGCTTGGCCG+TGG - chr5.2:25576674-25576693 MS.gene68336:intron 45.0%
!! ATGATTTTGAGGGCCTAAGG+AGG + chr5.2:25576250-25576269 None:intergenic 45.0%
!! CCAATGATTTTGAGGGCCTA+AGG + chr5.2:25576253-25576272 None:intergenic 45.0%
!! CTTTGCTGTCGCTGTCTTTT+TGG - chr5.2:25576035-25576054 MS.gene68336:intron 45.0%
!!! GGTGTTTTGGAAGTAGAGGA+AGG + chr5.2:25576346-25576365 None:intergenic 45.0%
AAGCCAATGACAATGGTGGC+TGG + chr5.2:25576018-25576037 None:intergenic 50.0%
ACACTGGTATCGTAACAGCG+TGG + chr5.2:25575787-25575806 None:intergenic 50.0%
ACGATGTTGAGGGGTAACAC+TGG + chr5.2:25575803-25575822 None:intergenic 50.0%
ATGGTGGCTGGAACAAGAAC+AGG + chr5.2:25576006-25576025 None:intergenic 50.0%
CTTGGAACACTCACTGGACT+GGG - chr5.2:25575949-25575968 MS.gene68336:intron 50.0%
GAAACTACACATTCAGCCCC+TGG + chr5.2:25576856-25576875 None:intergenic 50.0%
GAACAAGAACAGGGCTGAAG+AGG + chr5.2:25575996-25576015 None:intergenic 50.0%
GGAAGTAGAGGAAGGATTCG+AGG + chr5.2:25576338-25576357 None:intergenic 50.0%
GGCACTGTCCTTGTTCTCAT+GGG - chr5.2:25576080-25576099 MS.gene68336:intron 50.0%
GGGCTGAAGAGGATGAAAAG+TGG + chr5.2:25575985-25576004 None:intergenic 50.0%
GGTGAAGAAATGCAGTTGGC+TGG - chr5.2:25576576-25576595 MS.gene68336:intron 50.0%
GTAATTACCGGCCTTCCTGT+AGG - chr5.2:25575892-25575911 MS.gene68336:CDS 50.0%
GTCCAGTGAGTGTTCCAAGA+AGG + chr5.2:25575948-25575967 None:intergenic 50.0%
GTTCCAGCCACCATTGTCAT+TGG - chr5.2:25576012-25576031 MS.gene68336:intron 50.0%
TCCCTTCTTGGAACACTCAC+TGG - chr5.2:25575943-25575962 MS.gene68336:intron 50.0%
TCTACAGGAGCAACTCGAGT+TGG - chr5.2:25576134-25576153 MS.gene68336:intron 50.0%
TCTTGGAACACTCACTGGAC+TGG - chr5.2:25575948-25575967 MS.gene68336:intron 50.0%
TGGCACTGTCCTTGTTCTCA+TGG - chr5.2:25576079-25576098 MS.gene68336:intron 50.0%
TGGTGAGTTCGGAGTTTGTG+AGG + chr5.2:25576305-25576324 None:intergenic 50.0%
TGGTGGCTGGAACAAGAACA+GGG + chr5.2:25576005-25576024 None:intergenic 50.0%
TTGGAACACTCACTGGACTG+GGG - chr5.2:25575950-25575969 MS.gene68336:intron 50.0%
! AACAGCTTATGGTGGAGTGG+TGG - chr5.2:25576160-25576179 MS.gene68336:intron 50.0%
! AACTCGAGTTGCTCCTGTAG+AGG + chr5.2:25576135-25576154 None:intergenic 50.0%
!! GTGTTGCAGGTTGGTGAGTT+CGG + chr5.2:25576316-25576335 None:intergenic 50.0%
!! TGTCGCTGTCTTTTTGGCGT+CGG - chr5.2:25576041-25576060 MS.gene68336:intron 50.0%
!!! TTTTGGCGTCGGCAGCTTTT+GGG - chr5.2:25576052-25576071 MS.gene68336:intron 50.0%
!!! TTTTTGGCGTCGGCAGCTTT+TGG - chr5.2:25576051-25576070 MS.gene68336:intron 50.0%
AAGGACGTTGATGCCGCATG+TGG - chr5.2:25576518-25576537 MS.gene68336:intron 55.0%
ATTACCGGCCTTCCTGTAGG+CGG - chr5.2:25575895-25575914 MS.gene68336:CDS 55.0%
CCAATCAGTGTCGCCACATG+CGG + chr5.2:25576534-25576553 None:intergenic 55.0%
CTGGTATCGTAACAGCGTGG+TGG + chr5.2:25575784-25575803 None:intergenic 55.0%
CTGTAGAGGCCCTTGATCCT+GGG + chr5.2:25576121-25576140 None:intergenic 55.0%
CTTGCTGGACTCTCCCTTCT+TGG - chr5.2:25575931-25575950 MS.gene68336:intron 55.0%
GGTGTACTGCTTGCACTTGC+TGG - chr5.2:25575916-25575935 MS.gene68336:intron 55.0%
GTGTAAGGCGAATCCTTGGG+AGG + chr5.2:25576660-25576679 None:intergenic 55.0%
GTTTGTGAGGACTCGACGAG+TGG + chr5.2:25576292-25576311 None:intergenic 55.0%
TGTAGAGGCCCTTGATCCTG+GGG + chr5.2:25576120-25576139 None:intergenic 55.0%
! ATTCCCTTCAGGAGGGGCAT+TGG - chr5.2:25577557-25577576 MS.gene68336:CDS 55.0%
! CGCATGTGGCGACACTGATT+GGG - chr5.2:25576532-25576551 MS.gene68336:intron 55.0%
! GAGGACTCGACGAGTGGTTT+TGG + chr5.2:25576286-25576305 None:intergenic 55.0%
! GCTTATGGTGGAGTGGTGGT+CGG - chr5.2:25576164-25576183 MS.gene68336:intron 55.0%
! TGGAACACTCACTGGACTGG+GGG - chr5.2:25575951-25575970 MS.gene68336:intron 55.0%
!! GGAAGGATTCGAGGTGTTGC+AGG + chr5.2:25576329-25576348 None:intergenic 55.0%
!! GGATTCGAGGTGTTGCAGGT+TGG + chr5.2:25576325-25576344 None:intergenic 55.0%
AGGCGAATCCTTGGGAGGAG+TGG + chr5.2:25576655-25576674 None:intergenic 60.0%
CCAGGATCAAGGGCCTCTAC+AGG - chr5.2:25576119-25576138 MS.gene68336:intron 60.0%
CCGCATGTGGCGACACTGAT+TGG - chr5.2:25576531-25576550 MS.gene68336:intron 60.0%
CCTGTAGAGGCCCTTGATCC+TGG + chr5.2:25576122-25576141 None:intergenic 60.0%
CGTCCAATGCCCCTCCTGAA+GGG + chr5.2:25577563-25577582 None:intergenic 60.0%
GCAAGCAGTACACCGCCTAC+AGG + chr5.2:25575910-25575929 None:intergenic 60.0%
GCAGTACACCGCCTACAGGA+AGG + chr5.2:25575906-25575925 None:intergenic 60.0%
GGCGAATCCTTGGGAGGAGT+GGG + chr5.2:25576654-25576673 None:intergenic 60.0%
TACACCGCCTACAGGAAGGC+CGG + chr5.2:25575902-25575921 None:intergenic 60.0%
TGCTTCACCCACTCCTCCCA+AGG - chr5.2:25576644-25576663 MS.gene68336:intron 60.0%
! GTCGGCAGCTTTTGGGCTGA+TGG - chr5.2:25576059-25576078 MS.gene68336:intron 60.0%
!! CCTTCAGGAGGGGCATTGGA+CGG - chr5.2:25577561-25577580 MS.gene68336:intron 60.0%
CCGTCCAATGCCCCTCCTGA+AGG + chr5.2:25577564-25577583 None:intergenic 65.0%
TGGTGGAGTGGTGGTCGGAG+TGG - chr5.2:25576169-25576188 MS.gene68336:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 25575766 25577727 25575766 ID=MS.gene68336
chr5.2 mRNA 25575766 25577727 25575766 ID=MS.gene68336.t1;Parent=MS.gene68336
chr5.2 exon 25577673 25577727 25577673 ID=MS.gene68336.t1.exon1;Parent=MS.gene68336.t1
chr5.2 CDS 25577673 25577727 25577673 ID=cds.MS.gene68336.t1;Parent=MS.gene68336.t1
chr5.2 exon 25576781 25577579 25576781 ID=MS.gene68336.t1.exon2;Parent=MS.gene68336.t1
chr5.2 CDS 25576781 25577579 25576781 ID=cds.MS.gene68336.t1;Parent=MS.gene68336.t1
chr5.2 exon 25575766 25575925 25575766 ID=MS.gene68336.t1.exon3;Parent=MS.gene68336.t1
chr5.2 CDS 25575766 25575925 25575766 ID=cds.MS.gene68336.t1;Parent=MS.gene68336.t1
Gene Sequence

>MS.gene68336

ATGTCAGAACTTACACCACCACGCTGTTACGATACCAGTGTTACCCCTCAACATCGTTATTAAGTTGGTGGAGTTACTACTCATTACTCAGAGAAAAGAAGTCCATCAACTTCTCAAGTGCTTGCCGTAATTACCGGCCTTCCTGTAGGCGGTGTACTGCTTGCACTTGCTGGACTCTCCCTTCTTGGAACACTCACTGGACTGGGGGTAACAAGCCCACTTTTCATCCTCTTCAGCCCTGTTCTTGTTCCAGCCACCATTGTCATTGGCTTTGCTGTCGCTGTCTTTTTGGCGTCGGCAGCTTTTGGGCTGATGGCACTGTCCTTGTTCTCATGGGTCATGAATTACATACCCCAGGATCAAGGGCCTCTACAGGAGCAACTCGAGTTGGAAAAACAGCTTATGGTGGAGTGGTGGTCGGAGTGGTGTAACATGAAAGTTTTCTTCAGTTCTATTACCATGGCAGCAATTCTTTCACTCTCCTCCTTAGGCCCTCAAAATCATTGGTTCAACATCACCAAAACCACTCGTCGAGTCCTCACAAACTCCGAACTCACCAACCTGCAACACCTCGAATCCTTCCTCTACTTCCAAAACACCAAATCCGGTTCAATTTTGGTTCGAGTAATGAGAGACAACGAAATTGACCCAATAGTCTGTTTACTCGCATATGCGTTCGCTGAATTGATGTTGTTCCCGAAATGTTATATCAACATGTTGAGATTCCTTATGAAACAATATTTGATTAAACGAAGGACGTTGATGCCGCATGTGGCGACACTGATTGGGTTTTATAGAGGAGTTGCTGATGGTGAAGAAATGCAGTTGGCTGGCACTGTTGAGATTTGTTTTGATAAAAATGGTGTTAATTCTTCTCGTGCTTCACCCACTCCTCCCAAGGATTCGCCTTACACTTATAGCTTGGCCGTGGATAAATCTCTTAGAAGGTAAGCATTATTTATTGCGTCCAAGTTGCTGAAATTTGCATTTCTTTGGTAAAATTAGCTCAAGTGTCATGAACCATAAAGAACTAATTTGACCAAGGGAATACATATTCTTGGTCTAATTTGATTGGCTATATGCATATCCAGGGGCTGAATGTGTAGTTTCGATTATTCAAAGATTAAGGCTGTATTTAGATTGGTTTATGAATAACTTATTTGAGCTTACCTACAAACTGAAACATTTTTGAGTATGTTTGTTCGAGCTTATGAAAATAGTTCAAAACATGTCCATAAGCTGATTTTAGCTTATTCCAACAAACTCCCAGGGATAGTTTATGAACACAATTTGCAAATTATATGAAAACAGCTTAGCCACATTTTCTCGTTGCTATTATAAGTAGCTTATACATAAGCGCTAAATTAAGTTGTTTATTCAAACATACCCAAAGTGTATCTATATGTGAAAGCTAGAACTACTAGATGCGGGTAAAATGGATATTCAGAAACTAGTTTGATTATTAATTGTTAACTCTTCTGCTAGATTATGTAGGATATTTCTTGGGTGAATTTTGGAGACAGCTTGTCAGTATTATATACTTACTACAATTTTAACTTTTCTGATCTTACAGCTAGATAAATATTTTCATCAGTAGCAATTTTAGTTGGGAAAACCTGCAATTTAAATAATTAAGCTACCAAGTTTAAGATTTAAGTAACCGAAAATATATTCTCGTCGCGAAATTCCCATAGCTAACATTTTCAAACACATGGTATGTCTTCTCATTAAGCAAAAGAAAAAAAATCGATTGTCTGTTTTAACTTTCTAAAAACGTGTTTCTGATATTTAATTCCCTTCAGGAGGGGCATTGGACGGCATATTTTAAAGGCAAGTGAGGAACTGATTTCTCGGATGAGTTCATCAAGAGAAGTTTACTTGCATTGCAGAATGATTGATGAAGCTCCACTTAACATGTATACAAAAGCTGATTACAAAGTTGTTACAACAGCAGTATTTTAG
Protein sequence

>MS.gene68336.t1

MSELTPPRCYDTSVTPQHRGVLLALAGLSLLGTLTGLGVTSPLFILFSPVLVPATIVIGFAVAVFLASAAFGLMALSLFSWVMNYIPQDQGPLQEQLELEKQLMVEWWSEWCNMKVFFSSITMAAILSLSSLGPQNHWFNITKTTRRVLTNSELTNLQHLESFLYFQNTKSGSILVRVMRDNEIDPIVCLLAYAFAELMLFPKCYINMLRFLMKQYLIKRRTLMPHVATLIGFYRGVADGEEMQLAGTVEICFDKNGVNSSRASPTPPKDSPYTYSLAVDKSLRRRGIGRHILKASEELISRMSSSREVYLHCRMIDEAPLNMYTKADYKVVTTAVF