AlfalfaGEDB | MS.gene68427

M. sativa cultivar XinJiangDaYe / MS.gene68427

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	XP_013469276.1	75.2	157	15	2	1	133	1	157	1.30E-52	215.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	Q9FJK3	34.0	156	79	2	1	132	1	156	3.2e-15	82.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	A0A072VMS9	75.2	157	15	2	1	133	1	157	9.5e-53	215.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene68427.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	MTR_1g090710	75.159	157	15	2	1	133	1	157	1.73e-76	224
MS.gene68427.t1	MTR_1g090697	60.897	156	38	1	1	133	1	156	2.03e-60	183
MS.gene68427.t1	MTR_5g047580	45.161	155	62	1	1	132	1	155	2.46e-39	130
MS.gene68427.t1	MTR_1g090783	46.753	154	59	1	3	133	2	155	1.07e-38	128
MS.gene68427.t1	MTR_5g047560	46.043	139	68	1	1	132	1	139	1.80e-35	119
MS.gene68427.t1	MTR_1g077390	41.935	155	67	1	1	132	1	155	1.04e-34	118
MS.gene68427.t1	MTR_1g084950	43.226	155	65	2	1	132	1	155	1.24e-31	110
MS.gene68427.t1	MTR_1g077320	41.290	155	68	2	1	132	1	155	2.95e-30	107
MS.gene68427.t1	MTR_4g028720	40.000	155	70	2	1	132	1	155	6.93e-29	103
MS.gene68427.t1	MTR_7g011950	39.355	155	71	2	1	132	1	155	7.70e-28	100
MS.gene68427.t1	MTR_1g077300	38.710	155	70	3	1	132	1	153	2.97e-23	89.0
MS.gene68427.t1	MTR_3g466890	36.129	155	75	2	3	133	2	156	1.52e-22	87.4
MS.gene68427.t1	MTR_3g466830	32.903	155	80	2	3	133	2	156	9.77e-20	80.1
MS.gene68427.t1	MTR_4g031910	35.443	158	77	3	1	133	1	158	1.13e-18	79.0
MS.gene68427.t1	MTR_3g466980	28.571	154	86	2	1	130	1	154	1.60e-18	78.2
MS.gene68427.t1	MTR_4g032620	27.848	158	89	3	1	133	1	158	2.32e-17	75.5
MS.gene68427.t1	MTR_5g075380	31.847	157	83	2	1	133	1	157	3.54e-17	73.6
MS.gene68427.t1	MTR_3g065100	30.128	156	85	3	1	132	1	156	2.09e-16	73.2
MS.gene68427.t1	MTR_1g033930	40.179	112	58	2	23	133	12	115	4.86e-16	69.3
MS.gene68427.t1	MTR_8g036130	28.125	160	88	3	1	133	1	160	7.09e-15	68.9
MS.gene68427.t1	MTR_4g063790	29.487	156	81	4	1	129	1	154	2.97e-14	65.9
MS.gene68427.t1	MTR_2g016210	28.205	156	87	3	1	131	1	156	5.91e-13	63.9
MS.gene68427.t1	MTR_3g466900	35.294	102	65	1	33	133	26	127	6.40e-13	61.6
MS.gene68427.t1	MTR_4g032260	33.974	156	78	3	1	131	1	156	1.86e-12	61.2
MS.gene68427.t1	MTR_3g467080	27.673	159	86	4	1	132	1	157	2.60e-12	60.8
MS.gene68427.t1	MTR_1g033790	37.500	112	61	2	23	133	12	115	2.93e-12	59.7
MS.gene68427.t1	MTR_3g466930	32.353	102	68	1	33	133	26	127	3.30e-12	59.7
MS.gene68427.t1	MTR_3g031240	27.673	159	88	4	1	133	1	158	3.84e-12	60.8
MS.gene68427.t1	MTR_1g033370	32.990	97	64	1	38	133	25	121	2.99e-11	57.0
MS.gene68427.t1	MTR_3g031100	27.215	158	88	4	1	132	1	157	3.03e-11	59.3
MS.gene68427.t1	MTR_2g035610	28.926	121	62	3	1	97	1	121	4.82e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	AT5G26630	29.487	156	86	3	1	132	1	156	1.87e-17	75.5
MS.gene68427.t1	AT5G48670	32.051	156	82	3	1	132	1	156	2.00e-14	68.6
MS.gene68427.t1	AT1G65300	29.221	154	85	2	3	132	2	155	4.01e-14	67.4
MS.gene68427.t1	AT1G65330	29.870	154	84	2	3	132	2	155	2.02e-13	65.5
MS.gene68427.t1	AT1G31640	26.174	149	87	2	3	128	2	150	3.49e-13	65.5
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	5.38e-12	60.8
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	1.37e-11	60.1
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	1.44e-11	60.1

Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCTCAATTATCTTAT+TGG	0.120149	1.3:-63437716	None:intergenic
ATTATCTTATTGGCTCCTTC+TGG	0.257805	1.3:-63437706	None:intergenic
TGCCTTCCTTGCTGCGTCAT+CGG	0.331092	1.3:-63437560	None:intergenic
TGCTGCGTCATCGGGAATAA+AGG	0.359895	1.3:-63437551	None:intergenic
GCCTTCCTTGCTGCGTCATC+GGG	0.360745	1.3:-63437559	None:intergenic
TCTGCATGATAAACCTTTCA+TGG	0.377114	1.3:-63437777	None:intergenic
TTCACCTTTCTTCTCGCCAT+TGG	0.379933	1.3:-63437523	None:intergenic
GAGAAGATTGATACACTTGA+TGG	0.449535	1.3:+63437973	MS.gene68427:CDS
TTATTGGCTCCTTCTGGATG+TGG	0.451748	1.3:-63437700	None:intergenic
ACTGAAGAAGTGAAGGATCT+TGG	0.461931	1.3:+63437916	MS.gene68427:CDS
TCAAAGCAAGGGACAAACTA+AGG	0.480536	1.3:+63437806	MS.gene68427:CDS
AAGCTACAATAAAAGAAAGA+AGG	0.492405	1.3:+63437582	MS.gene68427:CDS
GCAACCAATGGCGAGAAGAA+AGG	0.507401	1.3:+63437519	None:intergenic
ATGAGAATCGTGAGCAAGAG+TGG	0.520504	1.3:+63437839	MS.gene68427:CDS
TCCCGATGACGCAGCAAGGA+AGG	0.534188	1.3:+63437558	MS.gene68427:CDS
GCGTGCTGAGAAGAAGTTGA+AGG	0.552409	1.3:+63437942	MS.gene68427:CDS
AAAGAAGGGTCTCATAAAGA+AGG	0.555375	1.3:+63437597	MS.gene68427:CDS
TGCAGACGATTGTCAAAGCA+AGG	0.562423	1.3:+63437794	MS.gene68427:CDS
AGCTACAATAAAAGAAAGAA+GGG	0.567899	1.3:+63437583	MS.gene68427:CDS
CTAGAAATGTGAGCCATGAA+AGG	0.580946	1.3:+63437764	MS.gene68427:CDS
ACTGACTACTGAAGAAGTGA+AGG	0.595360	1.3:+63437909	MS.gene68427:CDS
TGAGAATCGTGAGCAAGAGT+GGG	0.628956	1.3:+63437840	MS.gene68427:CDS
GAGGTTTCAAAGTTTAGCTG+TGG	0.630642	1.3:+63437732	MS.gene68427:CDS
TTATTCCCGATGACGCAGCA+AGG	0.647670	1.3:+63437554	MS.gene68427:CDS
GAGGTGTGGCCACATCCAGA+AGG	0.649175	1.3:+63437691	MS.gene68427:CDS
GAGCCAATAAGATAATTGAG+AGG	0.656204	1.3:+63437713	MS.gene68427:CDS
TTGATTCTCAGACAGAGGTG+TGG	0.667220	1.3:+63437677	MS.gene68427:intron
GCAGACGATTGTCAAAGCAA+GGG	0.675137	1.3:+63437795	MS.gene68427:CDS
TGCTGAGAAGAAGTTGAAGG+AGG	0.675328	1.3:+63437945	MS.gene68427:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACCTCTCAATTATCTTAT+TGG	-	chr1.3:63437719-63437738	None:intergenic	25.0%
!	AAGCTACAATAAAAGAAAGA+AGG	+	chr1.3:63437582-63437601	MS.gene68427:CDS	25.0%
!	AGCTACAATAAAAGAAAGAA+GGG	+	chr1.3:63437583-63437602	MS.gene68427:CDS	25.0%
	AAAGAAGGGTCTCATAAAGA+AGG	+	chr1.3:63437597-63437616	MS.gene68427:CDS	35.0%
	CTGAAAATTATAGCACAAGC+TGG	-	chr1.3:63437652-63437671	None:intergenic	35.0%
	GAGAAGATTGATACACTTGA+TGG	+	chr1.3:63437973-63437992	MS.gene68427:CDS	35.0%
	GAGCCAATAAGATAATTGAG+AGG	+	chr1.3:63437713-63437732	MS.gene68427:CDS	35.0%
	TCTGCATGATAAACCTTTCA+TGG	-	chr1.3:63437780-63437799	None:intergenic	35.0%
!!	ATTATCTTATTGGCTCCTTC+TGG	-	chr1.3:63437709-63437728	None:intergenic	35.0%
	ACCTCTGTCTGAGAATCAAA+TGG	-	chr1.3:63437676-63437695	None:intergenic	40.0%
	ACTGAAGAAGTGAAGGATCT+TGG	+	chr1.3:63437916-63437935	MS.gene68427:CDS	40.0%
	ACTGACTACTGAAGAAGTGA+AGG	+	chr1.3:63437909-63437928	MS.gene68427:CDS	40.0%
	AGCTGGAACATCACAAAGAA+TGG	-	chr1.3:63437635-63437654	None:intergenic	40.0%
	CTAGAAATGTGAGCCATGAA+AGG	+	chr1.3:63437764-63437783	MS.gene68427:CDS	40.0%
	GAGGTTTCAAAGTTTAGCTG+TGG	+	chr1.3:63437732-63437751	MS.gene68427:CDS	40.0%
	TCAAAGCAAGGGACAAACTA+AGG	+	chr1.3:63437806-63437825	MS.gene68427:CDS	40.0%
	TCCATTTGATTCTCAGACAG+AGG	+	chr1.3:63437672-63437691	MS.gene68427:intron	40.0%
!!!	AGTGGGATCTTTTTTTGCTC+AGG	+	chr1.3:63437857-63437876	MS.gene68427:CDS	40.0%
	ATGAGAATCGTGAGCAAGAG+TGG	+	chr1.3:63437839-63437858	MS.gene68427:CDS	45.0%
	GCAGACGATTGTCAAAGCAA+GGG	+	chr1.3:63437795-63437814	MS.gene68427:CDS	45.0%
	TGAGAATCGTGAGCAAGAGT+GGG	+	chr1.3:63437840-63437859	MS.gene68427:CDS	45.0%
	TGCAGACGATTGTCAAAGCA+AGG	+	chr1.3:63437794-63437813	MS.gene68427:CDS	45.0%
	TGCTGAGAAGAAGTTGAAGG+AGG	+	chr1.3:63437945-63437964	MS.gene68427:CDS	45.0%
	TTGATTCTCAGACAGAGGTG+TGG	+	chr1.3:63437677-63437696	MS.gene68427:intron	45.0%
!!	TTATTGGCTCCTTCTGGATG+TGG	-	chr1.3:63437703-63437722	None:intergenic	45.0%
	GCGTGCTGAGAAGAAGTTGA+AGG	+	chr1.3:63437942-63437961	MS.gene68427:CDS	50.0%
	TGCTGCGTCATCGGGAATAA+AGG	-	chr1.3:63437554-63437573	None:intergenic	50.0%
	TTATTCCCGATGACGCAGCA+AGG	+	chr1.3:63437554-63437573	MS.gene68427:CDS	50.0%
	TGCCTTCCTTGCTGCGTCAT+CGG	-	chr1.3:63437563-63437582	None:intergenic	55.0%
	GAGGTGTGGCCACATCCAGA+AGG	+	chr1.3:63437691-63437710	MS.gene68427:CDS	60.0%
	GCCTTCCTTGCTGCGTCATC+GGG	-	chr1.3:63437562-63437581	None:intergenic	60.0%
	TCCCGATGACGCAGCAAGGA+AGG	+	chr1.3:63437558-63437577	MS.gene68427:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	63437526	63437999	63437526	ID=MS.gene68427
chr1.3	mRNA	63437526	63437999	63437526	ID=MS.gene68427.t1;Parent=MS.gene68427
chr1.3	exon	63437526	63437618	63437526	ID=MS.gene68427.t1.exon1;Parent=MS.gene68427.t1
chr1.3	CDS	63437526	63437618	63437526	ID=cds.MS.gene68427.t1;Parent=MS.gene68427.t1
chr1.3	exon	63437688	63437999	63437688	ID=MS.gene68427.t1.exon2;Parent=MS.gene68427.t1
chr1.3	CDS	63437688	63437999	63437688	ID=cds.MS.gene68427.t1;Parent=MS.gene68427.t1

Gene Sequence

>MS.gene68427

ATGGCGAGAAGAAAGGTGAAACTCGCCTTTATTCCCGATGACGCAGCAAGGAAGGCAAGCTACAATAAAAGAAAGAAGGGTCTCATAAAGAAGGTAAGTGAACTCACCATTCTTTGTGATGTTCCAGCTTGTGCTATAATTTTCAGTCCATTTGATTCTCAGACAGAGGTGTGGCCACATCCAGAAGGAGCCAATAAGATAATTGAGAGGTTTCAAAGTTTAGCTGTGGTAGATGAAACTAGAAATGTGAGCCATGAAAGGTTTATCATGCAGACGATTGTCAAAGCAAGGGACAAACTAAGGAAGTTGAAATATGAGAATCGTGAGCAAGAGTGGGATCTTTTTTTGCTCAGGAACTTGAAAAACAACAATGTGCTTGATGAACTGACTACTGAAGAAGTGAAGGATCTTGGTAAGCGTGCTGAGAAGAAGTTGAAGGAGGTTAATGAGAAGATTGATACACTTGATGGATGA

Protein sequence

>MS.gene68427.t1

MARRKVKLAFIPDDAARKASYNKRKKGLIKKTEVWPHPEGANKIIERFQSLAVVDETRNVSHERFIMQTIVKARDKLRKLKYENREQEWDLFLLRNLKNNNVLDELTTEEVKDLGKRAEKKLKEVNEKIDTLDG