AlfalfaGEDB | MS.gene68427

M. sativa cultivar XinJiangDaYe / MS.gene68427

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	XP_013469276.1	75.2	157	15	2	1	133	1	157	1.30E-52	215.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	Q9FJK3	34.0	156	79	2	1	132	1	156	3.2e-15	82.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	A0A072VMS9	75.2	157	15	2	1	133	1	157	9.5e-53	215.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene68427.t1	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	MTR_1g090710	75.159	157	15	2	1	133	1	157	1.73e-76	224
MS.gene68427.t1	MTR_1g090697	60.897	156	38	1	1	133	1	156	2.03e-60	183
MS.gene68427.t1	MTR_5g047580	45.161	155	62	1	1	132	1	155	2.46e-39	130
MS.gene68427.t1	MTR_1g090783	46.753	154	59	1	3	133	2	155	1.07e-38	128
MS.gene68427.t1	MTR_5g047560	46.043	139	68	1	1	132	1	139	1.80e-35	119
MS.gene68427.t1	MTR_1g077390	41.935	155	67	1	1	132	1	155	1.04e-34	118
MS.gene68427.t1	MTR_1g084950	43.226	155	65	2	1	132	1	155	1.24e-31	110
MS.gene68427.t1	MTR_1g077320	41.290	155	68	2	1	132	1	155	2.95e-30	107
MS.gene68427.t1	MTR_4g028720	40.000	155	70	2	1	132	1	155	6.93e-29	103
MS.gene68427.t1	MTR_7g011950	39.355	155	71	2	1	132	1	155	7.70e-28	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68427.t1	AT5G26630	29.487	156	86	3	1	132	1	156	1.87e-17	75.5
MS.gene68427.t1	AT5G48670	32.051	156	82	3	1	132	1	156	2.00e-14	68.6
MS.gene68427.t1	AT1G65300	29.221	154	85	2	3	132	2	155	4.01e-14	67.4
MS.gene68427.t1	AT1G65330	29.870	154	84	2	3	132	2	155	2.02e-13	65.5
MS.gene68427.t1	AT1G31640	26.174	149	87	2	3	128	2	150	3.49e-13	65.5
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	5.38e-12	60.8
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	1.37e-11	60.1
MS.gene68427.t1	AT3G05860	30.872	149	79	2	1	125	1	149	1.44e-11	60.1

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Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCTCAATTATCTTAT+TGG	0.120149	1.3:-63437716	None:intergenic
AAAGAAGGGTCTCATAAAGA+AGG	0.555375	1.3:+63437597	MS.gene68427:CDS
AAGCTACAATAAAAGAAAGA+AGG	0.492405	1.3:+63437582	MS.gene68427:CDS
ACTGAAGAAGTGAAGGATCT+TGG	0.461931	1.3:+63437916	MS.gene68427:CDS
ACTGACTACTGAAGAAGTGA+AGG	0.595360	1.3:+63437909	MS.gene68427:CDS
AGCTACAATAAAAGAAAGAA+GGG	0.567899	1.3:+63437583	MS.gene68427:CDS
ATGAGAATCGTGAGCAAGAG+TGG	0.520504	1.3:+63437839	MS.gene68427:CDS
ATTATCTTATTGGCTCCTTC+TGG	0.257805	1.3:-63437706	None:intergenic
CTAGAAATGTGAGCCATGAA+AGG	0.580946	1.3:+63437764	MS.gene68427:CDS
GAGAAGATTGATACACTTGA+TGG	0.449535	1.3:+63437973	MS.gene68427:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAGGGTCTCATAAAGA+AGG	+	chr1.3:63437597-63437616	MS.gene68427:CDS	35.0%
CTGAAAATTATAGCACAAGC+TGG	-	chr1.3:63437652-63437671	None:intergenic	35.0%
GAGAAGATTGATACACTTGA+TGG	+	chr1.3:63437973-63437992	MS.gene68427:CDS	35.0%
GAGCCAATAAGATAATTGAG+AGG	+	chr1.3:63437713-63437732	MS.gene68427:CDS	35.0%
TCTGCATGATAAACCTTTCA+TGG	-	chr1.3:63437780-63437799	None:intergenic	35.0%
ACCTCTGTCTGAGAATCAAA+TGG	-	chr1.3:63437676-63437695	None:intergenic	40.0%
ACTGAAGAAGTGAAGGATCT+TGG	+	chr1.3:63437916-63437935	MS.gene68427:CDS	40.0%
ACTGACTACTGAAGAAGTGA+AGG	+	chr1.3:63437909-63437928	MS.gene68427:CDS	40.0%
AGCTGGAACATCACAAAGAA+TGG	-	chr1.3:63437635-63437654	None:intergenic	40.0%
CTAGAAATGTGAGCCATGAA+AGG	+	chr1.3:63437764-63437783	MS.gene68427:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	63437526	63437999	63437526	ID=MS.gene68427
chr1.3	mRNA	63437526	63437999	63437526	ID=MS.gene68427.t1;Parent=MS.gene68427
chr1.3	exon	63437526	63437618	63437526	ID=MS.gene68427.t1.exon1;Parent=MS.gene68427.t1
chr1.3	CDS	63437526	63437618	63437526	ID=cds.MS.gene68427.t1;Parent=MS.gene68427.t1
chr1.3	exon	63437688	63437999	63437688	ID=MS.gene68427.t1.exon2;Parent=MS.gene68427.t1
chr1.3	CDS	63437688	63437999	63437688	ID=cds.MS.gene68427.t1;Parent=MS.gene68427.t1

Gene Sequence

>MS.gene68427

ATGGCGAGAAGAAAGGTGAAACTCGCCTTTATTCCCGATGACGCAGCAAGGAAGGCAAGCTACAATAAAAGAAAGAAGGGTCTCATAAAGAAGGTAAGTGAACTCACCATTCTTTGTGATGTTCCAGCTTGTGCTATAATTTTCAGTCCATTTGATTCTCAGACAGAGGTGTGGCCACATCCAGAAGGAGCCAATAAGATAATTGAGAGGTTTCAAAGTTTAGCTGTGGTAGATGAAACTAGAAATGTGAGCCATGAAAGGTTTATCATGCAGACGATTGTCAAAGCAAGGGACAAACTAAGGAAGTTGAAATATGAGAATCGTGAGCAAGAGTGGGATCTTTTTTTGCTCAGGAACTTGAAAAACAACAATGTGCTTGATGAACTGACTACTGAAGAAGTGAAGGATCTTGGTAAGCGTGCTGAGAAGAAGTTGAAGGAGGTTAATGAGAAGATTGATACACTTGATGGATGA

Protein sequence

>MS.gene68427.t1

MARRKVKLAFIPDDAARKASYNKRKKGLIKKTEVWPHPEGANKIIERFQSLAVVDETRNVSHERFIMQTIVKARDKLRKLKYENREQEWDLFLLRNLKNNNVLDELTTEEVKDLGKRAEKKLKEVNEKIDTLDG