AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68432

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68432.t1 MTR_1g090697 92.308 156 12 0 1 156 1 156 1.02e-103 293
MS.gene68432.t1 MTR_1g090710 71.338 157 44 1 1 156 1 157 5.39e-79 231
MS.gene68432.t1 MTR_5g047580 60.000 155 62 0 1 155 1 155 3.31e-63 191
MS.gene68432.t1 MTR_1g090783 61.039 154 60 0 3 156 2 155 4.58e-62 188
MS.gene68432.t1 MTR_1g084950 63.871 155 56 0 1 155 1 155 2.88e-61 186
MS.gene68432.t1 MTR_1g077320 61.290 155 60 0 1 155 1 155 2.00e-59 181
MS.gene68432.t1 MTR_1g077390 57.419 155 66 0 1 155 1 155 2.04e-58 179
MS.gene68432.t1 MTR_4g028720 60.000 155 62 0 1 155 1 155 5.02e-57 176
MS.gene68432.t1 MTR_7g011950 60.000 155 62 0 1 155 1 155 6.45e-57 175
MS.gene68432.t1 MTR_1g077300 56.129 155 66 1 1 155 1 153 5.96e-48 152
MS.gene68432.t1 MTR_5g047560 52.258 155 58 1 1 155 1 139 7.58e-48 152
MS.gene68432.t1 MTR_3g466830 43.226 155 87 1 3 156 2 156 3.99e-39 130
MS.gene68432.t1 MTR_3g466890 41.290 155 90 1 3 156 2 156 1.27e-36 124
MS.gene68432.t1 MTR_4g032620 37.342 158 97 2 1 156 1 158 4.35e-36 124
MS.gene68432.t1 MTR_3g065100 41.667 156 90 1 1 155 1 156 1.07e-34 121
MS.gene68432.t1 MTR_3g466980 35.714 154 98 1 1 153 1 154 5.38e-34 119
MS.gene68432.t1 MTR_4g031910 41.139 158 91 2 1 156 1 158 2.52e-32 115
MS.gene68432.t1 MTR_8g036130 36.875 160 97 3 1 156 1 160 4.40e-31 112
MS.gene68432.t1 MTR_2g016210 41.026 156 90 2 1 154 1 156 6.17e-31 112
MS.gene68432.t1 MTR_5g075380 35.669 157 100 1 1 156 1 157 1.26e-29 106
MS.gene68432.t1 MTR_4g032260 40.385 156 91 2 1 154 1 156 1.75e-27 100
MS.gene68432.t1 MTR_2g035610 42.149 121 69 1 1 120 1 121 2.23e-27 99.4
MS.gene68432.t1 MTR_4g063790 33.333 156 98 3 1 152 1 154 4.26e-27 100
MS.gene68432.t1 MTR_3g466900 40.476 126 74 1 32 156 2 127 5.82e-27 98.6
MS.gene68432.t1 MTR_3g031240 32.911 158 104 2 1 156 1 158 1.08e-26 99.4
MS.gene68432.t1 MTR_4g032290 39.873 158 93 2 1 156 1 158 3.32e-25 96.7
MS.gene68432.t1 MTR_3g466930 38.095 126 77 1 32 156 2 127 8.11e-25 93.2
MS.gene68432.t1 MTR_3g031100 32.484 157 104 2 1 155 1 157 3.08e-24 94.4
MS.gene68432.t1 MTR_4g028800 51.389 72 33 1 1 72 1 70 4.21e-23 87.0
MS.gene68432.t1 MTR_3g467080 28.662 157 110 2 1 155 1 157 5.50e-20 81.6
MS.gene68432.t1 MTR_4g019670 29.448 163 97 4 1 145 1 163 2.27e-17 78.2
MS.gene68432.t1 MTR_2g035580 28.387 155 110 1 1 154 1 155 4.28e-17 73.9
MS.gene68432.t1 MTR_1g033930 46.809 94 49 1 64 156 22 115 5.86e-17 72.4
MS.gene68432.t1 MTR_7g106510 30.488 164 94 6 1 145 1 163 5.96e-17 77.0
MS.gene68432.t1 MTR_3g093900 29.677 155 108 1 3 156 6 160 1.62e-16 75.9
MS.gene68432.t1 MTR_1g033370 38.776 98 59 1 60 156 24 121 2.89e-15 68.2
MS.gene68432.t1 MTR_3g465410 28.906 128 85 3 29 152 1 126 5.28e-14 65.5
MS.gene68432.t1 MTR_7g055800 29.661 118 56 2 1 118 1 91 7.35e-14 63.5
MS.gene68432.t1 MTR_1g033790 43.617 94 52 1 64 156 22 115 9.15e-14 64.3
MS.gene68432.t1 MTR_6g005450 29.688 128 77 4 1 115 1 128 4.48e-11 60.1
MS.gene68432.t1 MTR_7g075850 31.373 153 86 4 1 148 1 139 6.60e-11 58.9
MS.gene68432.t1 MTR_6g018920 24.224 161 109 6 1 155 1 154 7.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68432.t1 AT3G05860 41.611 149 86 1 1 148 1 149 1.96e-32 114
MS.gene68432.t1 AT3G05860 41.611 149 86 1 1 148 1 149 1.15e-31 114
MS.gene68432.t1 AT3G05860 41.611 149 86 1 1 148 1 149 1.49e-31 114
MS.gene68432.t1 AT5G48670 42.949 156 88 1 1 155 1 156 7.80e-31 113
MS.gene68432.t1 AT1G65300 35.714 154 98 1 3 155 2 155 9.52e-31 112
MS.gene68432.t1 AT5G26630 37.179 156 97 1 1 155 1 156 2.56e-28 104
MS.gene68432.t1 AT1G65330 35.714 154 98 1 3 155 2 155 3.04e-28 105
MS.gene68432.t1 AT1G22590 37.342 158 93 3 1 156 1 154 1.63e-26 98.2
MS.gene68432.t1 AT1G31640 36.000 150 94 2 3 151 2 150 1.90e-24 98.2
MS.gene68432.t1 AT5G26650 33.987 153 100 1 4 155 2 154 9.31e-24 95.5
MS.gene68432.t1 AT1G31630 30.464 151 103 2 3 152 2 151 3.31e-23 93.6
MS.gene68432.t1 AT5G27960 32.680 153 102 1 4 155 2 154 3.32e-22 90.5
MS.gene68432.t1 AT5G27810 40.625 96 56 1 29 123 1 96 7.31e-20 80.1
MS.gene68432.t1 AT2G28700 33.071 127 78 2 1 123 1 124 9.94e-20 84.0
MS.gene68432.t1 AT5G06500 35.526 152 84 5 1 145 1 145 6.30e-19 80.5
MS.gene68432.t1 AT5G26580 28.387 155 98 1 1 155 1 142 3.46e-15 71.6
MS.gene68432.t1 AT2G40210 32.857 140 88 3 1 137 1 137 5.15e-12 62.8

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACCTTCTTGGCTTCTTC+TGG 0.189716 1.1:+66198839 None:intergenic
ATACCTCTCAATTACCTTCT+TGG 0.215921 1.1:+66198829 None:intergenic
TGCCTTCCTTGCCGCGTTAT+CGG 0.274204 1.1:+66198985 None:intergenic
GACAACACTTTGTGATATTC+CGG 0.300516 1.1:-66198915 MS.gene68432:CDS
TTCTTGGCTTCTTCTGGATT+CGG 0.341741 1.1:+66198845 None:intergenic
TCTGCAGAATGTATCTTTCA+TGG 0.389169 1.1:+66198768 None:intergenic
TTGATTCTCAGACTGAGGTT+TGG 0.414602 1.1:-66198868 MS.gene68432:CDS
TGCCGCGTTATCGGAGATAA+AGG 0.416769 1.1:+66198994 None:intergenic
CTCCGATAACGCGGCAAGGA+AGG 0.449540 1.1:-66198987 MS.gene68432:CDS
TTTCACCTTCCTTCTCGTCA+TGG 0.513068 1.1:+66199021 None:intergenic
ACCTCAGTCTGAGAATCAAA+AGG 0.514840 1.1:+66198872 None:intergenic
GCATGTTGAGAAGAAGTTGA+AGG 0.520532 1.1:-66198603 MS.gene68432:CDS
AGTCTGAGAATCAAAAGGAC+TGG 0.521393 1.1:+66198877 None:intergenic
AAGCTACAATAAAAGAGAGA+AGG 0.529044 1.1:-66198963 MS.gene68432:CDS
AGAGAAGGGTCTCATAAAGA+AGG 0.536116 1.1:-66198948 MS.gene68432:CDS
TGCAGATGATTGCTAAAGCA+AGG 0.540172 1.1:-66198751 MS.gene68432:CDS
AGCTACAATAAAAGAGAGAA+GGG 0.565757 1.1:-66198962 MS.gene68432:CDS
TGTTGAGAAGAAGTTGAAGG+AGG 0.578028 1.1:-66198600 MS.gene68432:CDS
TGCAACCATGACGAGAAGGA+AGG 0.595441 1.1:-66199026 None:intergenic
GAATCCAGAAGAAGCCAAGA+AGG 0.598410 1.1:-66198843 MS.gene68432:CDS
TGCCTTTATCTCCGATAACG+CGG 0.615017 1.1:-66198996 MS.gene68432:CDS
TTATCTCCGATAACGCGGCA+AGG 0.616944 1.1:-66198991 MS.gene68432:CDS
CTGGAAATTATAGCACAAGC+CGG 0.619015 1.1:+66198896 None:intergenic
GCAGATGATTGCTAAAGCAA+GGG 0.625022 1.1:-66198750 MS.gene68432:CDS
AAGCCAAGAAGGTAATTGAG+AGG 0.625836 1.1:-66198832 MS.gene68432:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCTACAATAAAAGAGAGA+AGG - chr1.1:66198627-66198646 MS.gene68432:CDS 30.0%
AGCTACAATAAAAGAGAGAA+GGG - chr1.1:66198628-66198647 MS.gene68432:CDS 30.0%
! AGCTGAATCTTTTTATGCTA+CGG - chr1.1:66198902-66198921 MS.gene68432:CDS 30.0%
ATACCTCTCAATTACCTTCT+TGG + chr1.1:66198764-66198783 None:intergenic 35.0%
GACAACACTTTGTGATATTC+CGG - chr1.1:66198675-66198694 MS.gene68432:CDS 35.0%
TCTGCAGAATGTATCTTTCA+TGG + chr1.1:66198825-66198844 None:intergenic 35.0%
! TTTTCAGTTCTTCAGCAGTT+AGG + chr1.1:66198957-66198976 None:intergenic 35.0%
AAGCCAAGAAGGTAATTGAG+AGG - chr1.1:66198758-66198777 MS.gene68432:CDS 40.0%
ACCTCAGTCTGAGAATCAAA+AGG + chr1.1:66198721-66198740 None:intergenic 40.0%
AGAGAAGGGTCTCATAAAGA+AGG - chr1.1:66198642-66198661 MS.gene68432:CDS 40.0%
AGTCTGAGAATCAAAAGGAC+TGG + chr1.1:66198716-66198735 None:intergenic 40.0%
ATTACCTTCTTGGCTTCTTC+TGG + chr1.1:66198754-66198773 None:intergenic 40.0%
CTGGAAATTATAGCACAAGC+CGG + chr1.1:66198697-66198716 None:intergenic 40.0%
GCAGATGATTGCTAAAGCAA+GGG - chr1.1:66198840-66198859 MS.gene68432:CDS 40.0%
GCATGTTGAGAAGAAGTTGA+AGG - chr1.1:66198987-66199006 MS.gene68432:CDS 40.0%
TGCAGATGATTGCTAAAGCA+AGG - chr1.1:66198839-66198858 MS.gene68432:CDS 40.0%
TGTTGAGAAGAAGTTGAAGG+AGG - chr1.1:66198990-66199009 MS.gene68432:CDS 40.0%
TTCTTGGCTTCTTCTGGATT+CGG + chr1.1:66198748-66198767 None:intergenic 40.0%
TTGATTCTCAGACTGAGGTT+TGG - chr1.1:66198722-66198741 MS.gene68432:CDS 40.0%
!! TCCTTTTGATTCTCAGACTG+AGG - chr1.1:66198717-66198736 MS.gene68432:CDS 40.0%
GAATCCAGAAGAAGCCAAGA+AGG - chr1.1:66198747-66198766 MS.gene68432:CDS 45.0%
TGCCTTTATCTCCGATAACG+CGG - chr1.1:66198594-66198613 MS.gene68432:CDS 45.0%
TGCCGCGTTATCGGAGATAA+AGG + chr1.1:66198599-66198618 None:intergenic 50.0%
TTATCTCCGATAACGCGGCA+AGG - chr1.1:66198599-66198618 MS.gene68432:CDS 50.0%
TGCCTTCCTTGCCGCGTTAT+CGG + chr1.1:66198608-66198627 None:intergenic 55.0%
CTCCGATAACGCGGCAAGGA+AGG - chr1.1:66198603-66198622 MS.gene68432:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 66198571 66199041 66198571 ID=MS.gene68432
chr1.1 mRNA 66198571 66199041 66198571 ID=MS.gene68432.t1;Parent=MS.gene68432
chr1.1 exon 66198571 66199041 66198571 ID=MS.gene68432.t1.exon1;Parent=MS.gene68432.t1
chr1.1 CDS 66198571 66199041 66198571 ID=cds.MS.gene68432.t1;Parent=MS.gene68432.t1
Gene Sequence

>MS.gene68432

ATGACGAGAAGGAAGGTGAAACTTGCCTTTATCTCCGATAACGCGGCAAGGAAGGCAAGCTACAATAAAAGAGAGAAGGGTCTCATAAAGAAGGTAACTGAACTGACAACACTTTGTGATATTCCGGCTTGTGCTATAATTTCCAGTCCTTTTGATTCTCAGACTGAGGTTTGGCCGAATCCAGAAGAAGCCAAGAAGGTAATTGAGAGGTATCAAAGTTATTCTTTGATAGATGAAAACAAGAATGTGAGCCATGAAAGATACATTCTGCAGATGATTGCTAAAGCAAGGGACAAACTAAGAAAGTTGAAGCATGACAATCGTGAGCAAGAGCTGAATCTTTTTATGCTACGGAACTTGAAAAATAACAATTTGTATGAAGACCTAACTGCTGAAGAACTGAAAAATCTTGCTAAGCATGTTGAGAAGAAGTTGAAGGAGGTTGATGACAAGATTGATACACTTGATTGA
Protein sequence

>MS.gene68432.t1

MTRRKVKLAFISDNAARKASYNKREKGLIKKVTELTTLCDIPACAIISSPFDSQTEVWPNPEEAKKVIERYQSYSLIDENKNVSHERYILQMIAKARDKLRKLKHDNREQELNLFMLRNLKNNNLYEDLTAEELKNLAKHVEKKLKEVDDKIDTLD