AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68486

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68486.t1 MTR_3g056110 96.828 536 17 0 2 537 7 542 0.0 1083
MS.gene68486.t1 MTR_5g094450 67.587 543 152 9 2 537 7 532 0.0 726
MS.gene68486.t1 MTR_5g097480 58.256 539 213 5 8 537 9 544 0.0 647
MS.gene68486.t1 MTR_4g133660 49.084 546 240 8 11 536 15 542 1.07e-177 512
MS.gene68486.t1 MTR_2g082090 58.871 372 153 0 165 536 207 578 8.82e-169 490
MS.gene68486.t1 MTR_2g026250 46.753 539 230 7 51 537 63 596 6.43e-166 484
MS.gene68486.t1 MTR_1g029420 50.000 370 185 0 168 537 223 592 4.82e-141 420
MS.gene68486.t1 MTR_3g089055 49.081 381 193 1 157 537 191 570 6.21e-138 411
MS.gene68486.t1 MTR_3g089055 49.081 381 193 1 157 537 186 565 1.06e-137 410
MS.gene68486.t1 MTR_7g027190 33.575 551 298 13 16 537 162 673 2.36e-78 259
MS.gene68486.t1 MTR_3g065980 35.476 389 216 11 167 537 169 540 8.85e-64 218
MS.gene68486.t1 MTR_7g062120 30.319 376 255 4 166 537 368 740 6.72e-62 216
MS.gene68486.t1 MTR_4g064160 32.000 375 248 4 166 536 312 683 6.76e-62 216
MS.gene68486.t1 MTR_4g064150 32.540 378 246 6 166 536 357 732 1.61e-61 215
MS.gene68486.t1 MTR_8g442410 32.552 384 238 7 166 537 151 525 2.51e-61 211
MS.gene68486.t1 MTR_2g097390 29.388 490 309 12 72 536 163 640 7.15e-61 212
MS.gene68486.t1 MTR_2g097350 29.191 507 310 14 56 536 156 639 2.43e-59 208
MS.gene68486.t1 MTR_2g097410 31.496 381 244 8 166 536 367 740 2.61e-59 209
MS.gene68486.t1 MTR_6g047750 37.464 347 188 8 205 537 331 662 3.35e-59 208
MS.gene68486.t1 MTR_7g057230 34.897 341 208 6 203 536 323 656 7.90e-59 207
MS.gene68486.t1 MTR_2g097310 29.613 439 284 7 117 536 205 637 1.18e-58 206
MS.gene68486.t1 MTR_4g064120 31.016 374 253 3 166 534 250 623 7.12e-58 203
MS.gene68486.t1 MTR_4g064180 31.635 373 250 3 166 533 250 622 2.84e-57 202
MS.gene68486.t1 MTR_7g074650 34.247 365 225 6 174 537 442 792 1.78e-56 202
MS.gene68486.t1 MTR_4g104020 29.688 384 248 5 167 537 146 520 1.77e-55 195
MS.gene68486.t1 MTR_4g064200 31.733 375 247 7 166 536 280 649 1.98e-55 197
MS.gene68486.t1 MTR_4g097080 29.114 395 253 9 158 537 121 503 2.26e-54 191
MS.gene68486.t1 MTR_2g097467 29.177 401 277 4 137 536 259 653 2.31e-53 192
MS.gene68486.t1 MTR_3g022830 27.578 417 246 5 171 537 26 436 5.11e-53 186
MS.gene68486.t1 MTR_4g077760 35.311 354 212 10 190 537 213 555 6.70e-51 183
MS.gene68486.t1 MTR_4g102790 31.122 392 236 13 164 536 285 661 5.71e-50 182
MS.gene68486.t1 MTR_2g097463 29.301 372 259 2 166 536 286 654 1.65e-49 181
MS.gene68486.t1 MTR_2g097473 29.759 373 256 5 166 536 285 653 3.47e-49 180
MS.gene68486.t1 MTR_2g097380 26.793 474 298 12 72 536 127 560 6.36e-48 175
MS.gene68486.t1 MTR_4g076020 32.432 370 216 10 174 537 88 429 1.60e-47 171
MS.gene68486.t1 MTR_5g015490 29.412 391 239 12 170 537 114 490 6.87e-47 171
MS.gene68486.t1 MTR_1g096030 28.993 407 229 12 167 537 77 459 1.94e-43 160
MS.gene68486.t1 MTR_1g069725 30.769 377 236 12 166 536 105 462 7.55e-43 159
MS.gene68486.t1 MTR_5g058860 31.694 366 223 11 189 536 140 496 2.95e-40 152
MS.gene68486.t1 MTR_3g072710 30.376 372 222 10 189 536 141 499 3.22e-40 152
MS.gene68486.t1 MTR_2g089100 25.118 422 272 9 149 536 31 442 8.52e-39 147
MS.gene68486.t1 MTR_2g099110 27.551 392 248 10 169 537 73 451 1.28e-35 139
MS.gene68486.t1 MTR_2g034250 27.506 389 254 9 167 537 207 585 6.20e-35 139
MS.gene68486.t1 MTR_5g015950 24.824 427 251 11 167 536 32 445 1.46e-32 130
MS.gene68486.t1 MTR_3g053270 24.824 427 251 11 167 536 32 445 1.46e-32 130
MS.gene68486.t1 MTR_4g095500 24.586 423 270 9 161 536 47 467 2.98e-32 129
MS.gene68486.t1 MTR_0092s0100 27.200 375 250 8 169 537 374 731 4.89e-32 131
MS.gene68486.t1 MTR_0092s0100 27.200 375 250 8 169 537 371 728 5.82e-32 131
MS.gene68486.t1 MTR_2g034280 26.706 337 232 6 167 494 200 530 4.86e-31 127
MS.gene68486.t1 MTR_4g026485 25.521 384 244 12 169 537 268 624 4.22e-30 125
MS.gene68486.t1 MTR_7g109580 24.672 381 267 9 166 537 185 554 6.07e-29 120
MS.gene68486.t1 MTR_2g034260 25.369 339 236 6 167 494 207 539 1.58e-28 120
MS.gene68486.t1 MTR_4g076140 30.045 223 148 3 155 375 32 248 3.77e-28 117
MS.gene68486.t1 MTR_4g076140 37.589 141 87 1 398 537 329 469 2.92e-26 112
MS.gene68486.t1 MTR_5g009080 28.774 212 144 2 167 377 53 258 6.55e-27 114
MS.gene68486.t1 MTR_5g009080 34.752 141 91 1 398 537 338 478 3.13e-23 103
MS.gene68486.t1 MTR_8g093070 28.212 397 246 17 155 536 123 495 9.52e-27 114
MS.gene68486.t1 MTR_1g086970 25.143 350 239 9 201 537 139 478 7.88e-26 110
MS.gene68486.t1 MTR_8g020840 25.310 403 245 15 168 537 169 548 7.56e-24 105
MS.gene68486.t1 MTR_8g077940 23.969 388 255 14 166 537 178 541 4.22e-17 84.7
MS.gene68486.t1 MTR_3g022580 30.894 123 84 1 415 537 62 183 1.28e-14 72.8
MS.gene68486.t1 MTR_3g022005 35.484 93 60 0 445 537 33 125 2.25e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68486.t1 AT1G50600 56.192 541 213 7 1 537 77 597 0.0 586
MS.gene68486.t1 AT1G50600 56.192 541 213 7 1 537 81 601 0.0 586
MS.gene68486.t1 AT1G50600 56.192 541 213 7 1 537 6 526 0.0 585
MS.gene68486.t1 AT5G48150 64.554 426 148 2 113 537 67 490 0.0 580
MS.gene68486.t1 AT5G48150 64.554 426 148 2 113 537 67 490 0.0 580
MS.gene68486.t1 AT5G48150 64.554 426 148 2 113 537 67 490 0.0 580
MS.gene68486.t1 AT5G48150 64.554 426 148 2 113 537 67 490 0.0 580
MS.gene68486.t1 AT2G04890 58.937 414 163 1 124 537 7 413 0.0 518
MS.gene68486.t1 AT4G17230 50.000 526 232 10 22 537 21 525 1.09e-167 486
MS.gene68486.t1 AT4G17230 50.000 526 232 10 22 537 21 525 1.09e-167 486
MS.gene68486.t1 AT1G21450 42.231 502 266 5 58 537 94 593 5.78e-136 407
MS.gene68486.t1 AT1G66350 36.533 375 214 10 167 537 152 506 1.13e-68 230
MS.gene68486.t1 AT5G17490 36.074 377 218 10 167 537 157 516 6.55e-68 228
MS.gene68486.t1 AT5G52510 38.218 348 190 7 205 537 303 640 2.38e-67 229
MS.gene68486.t1 AT1G07530 33.690 374 246 2 165 536 391 764 1.00e-66 230
MS.gene68486.t1 AT1G14920 35.433 381 216 10 167 537 169 529 2.53e-66 224
MS.gene68486.t1 AT3G03450 35.200 375 230 8 167 537 180 545 3.74e-65 221
MS.gene68486.t1 AT2G01570 35.904 376 221 9 167 537 221 581 2.44e-64 220
MS.gene68486.t1 AT2G29060 32.800 375 248 3 166 536 280 654 4.89e-63 218
MS.gene68486.t1 AT2G29060 32.800 375 248 3 166 536 317 691 1.10e-62 218
MS.gene68486.t1 AT5G59450 28.191 376 266 3 166 537 223 598 8.72e-55 195
MS.gene68486.t1 AT3G46600 30.108 372 258 2 167 537 1 371 9.21e-55 189
MS.gene68486.t1 AT3G46600 30.295 373 258 2 166 537 208 579 2.04e-54 193
MS.gene68486.t1 AT3G46600 30.295 373 258 2 166 537 176 547 2.50e-54 192
MS.gene68486.t1 AT5G66770 33.952 377 234 8 167 537 217 584 3.87e-54 192
MS.gene68486.t1 AT2G29065 31.332 383 248 8 166 536 248 627 1.65e-53 192
MS.gene68486.t1 AT3G54220 31.235 413 265 8 129 536 251 649 3.91e-53 191
MS.gene68486.t1 AT2G37650 28.989 376 258 5 166 537 331 701 1.09e-52 191
MS.gene68486.t1 AT2G37650 28.989 376 258 5 166 537 343 713 1.24e-52 191
MS.gene68486.t1 AT3G50650 34.986 363 218 7 184 537 189 542 2.95e-52 186
MS.gene68486.t1 AT1G50420 27.483 433 245 8 167 537 54 479 1.17e-51 184
MS.gene68486.t1 AT1G07520 29.676 401 269 7 145 536 132 528 9.13e-51 182
MS.gene68486.t1 AT1G07520 29.676 401 269 7 145 536 132 528 9.13e-51 182
MS.gene68486.t1 AT1G07520 29.676 401 269 7 145 536 296 692 2.78e-50 184
MS.gene68486.t1 AT1G07520 29.676 401 269 7 145 536 297 693 3.11e-50 184
MS.gene68486.t1 AT5G41920 32.637 383 224 11 167 537 40 400 1.11e-49 176
MS.gene68486.t1 AT4G37650 27.525 396 247 11 170 537 146 529 1.64e-44 165
MS.gene68486.t1 AT1G55580 28.019 414 246 9 167 537 41 445 1.51e-42 158
MS.gene68486.t1 AT1G63100 30.591 389 244 7 166 536 273 653 2.52e-42 161
MS.gene68486.t1 AT1G63100 30.591 389 244 7 166 536 248 628 2.87e-42 160
MS.gene68486.t1 AT3G49950 25.253 396 255 9 167 536 27 407 4.68e-36 139
MS.gene68486.t1 AT4G08250 29.412 306 200 6 239 536 179 476 3.12e-34 135
MS.gene68486.t1 AT3G60630 28.808 302 188 9 249 537 335 622 5.29e-26 112
MS.gene68486.t1 AT2G45160 25.562 356 217 13 202 537 312 639 1.21e-24 108
MS.gene68486.t1 AT4G00150 25.064 391 232 16 167 537 205 554 1.22e-23 105
MS.gene68486.t1 AT3G13840 23.725 451 294 14 120 537 77 510 9.95e-22 99.0
MS.gene68486.t1 AT4G36710 24.557 395 245 14 166 537 121 485 7.09e-20 93.2
MS.gene68486.t1 AT5G67411 29.082 196 122 6 290 474 9 198 1.48e-14 73.2

Find 116 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACCTGTTATTCTAATCTT+TGG 0.235841 3.2:+48502438 None:intergenic
CGCAAAATAGTATCCGTTTC+AGG 0.240168 3.2:-48502765 MS.gene68486:CDS
CTGCAAGAACGGAGCGTTAT+TGG 0.252017 3.2:+48502110 None:intergenic
GTGCACCATCTCTCTCTTCT+AGG 0.256808 3.2:+48501806 None:intergenic
ATCTGCTTCGTTCTTATTAG+AGG 0.270897 3.2:-48502913 MS.gene68486:CDS
GTTTCAAAGATTGCCTCTTC+TGG 0.289813 3.2:-48502699 MS.gene68486:CDS
CAGGCTCTTGCTCGCCGGCC+TGG 0.315935 3.2:-48502468 MS.gene68486:CDS
ATCTTATTGTTTCTTGTGCT+TGG 0.342571 3.2:-48501759 MS.gene68486:CDS
AGCAGATTCTTCTTGAACTT+TGG 0.342587 3.2:+48502929 None:intergenic
CCTCCAAAGATTAGAATAAC+AGG 0.365174 3.2:-48502441 MS.gene68486:CDS
GATGAGAAGGATAGAATGTT+TGG 0.365693 3.2:+48503225 None:intergenic
ATATGTCCGTACTTTAAATT+CGG 0.373248 3.2:-48502597 MS.gene68486:CDS
AGAGATGGTGCACTGTTTCT+TGG 0.374714 3.2:-48501796 MS.gene68486:CDS
CAGGCCGGCGAGCAAGAGCC+TGG 0.383296 3.2:+48502469 None:intergenic
CATTCACTTGAATTTGTCAA+TGG 0.402839 3.2:-48503074 MS.gene68486:CDS
TTCACCGCCACAGCTTCATT+AGG 0.419761 3.2:+48502261 None:intergenic
TAATGTCGAACAGCACTGTT+TGG 0.423158 3.2:-48502004 MS.gene68486:CDS
GTAGAACCTGGACTATTGTT+AGG 0.425781 3.2:+48503110 None:intergenic
CTTGATTGAAGAATTGATGA+AGG 0.426007 3.2:+48501861 None:intergenic
ATCCTCTCTTTGTGATCCCT+TGG 0.427208 3.2:+48502027 None:intergenic
AACATCAATTGATTCATAAA+CGG 0.428207 3.2:+48502056 None:intergenic
CAGAAACATGAAATTTCTTA+CGG 0.431673 3.2:-48503347 MS.gene68486:CDS
CCTGTTATTCTAATCTTTGG+AGG 0.435815 3.2:+48502441 None:intergenic
GCTCACTCATCTTATGCCTT+AGG 0.446845 3.2:+48503003 None:intergenic
TCATCGTATGCCCGCGGTGG+GGG 0.447317 3.2:-48502402 MS.gene68486:CDS
CAATTGATGTTGTTCTTCCA+AGG 0.452748 3.2:-48502044 MS.gene68486:CDS
TTCGGATATATGTCTGCAAA+TGG 0.452855 3.2:-48502579 MS.gene68486:CDS
GGCGAGCAAGAGCCTGGATT+AGG 0.464253 3.2:+48502475 None:intergenic
ATGAAGGAGCTCAATGGATA+AGG 0.465572 3.2:+48501877 None:intergenic
AGGCAGTCCAATCGAACGAT+TGG 0.470118 3.2:-48502745 MS.gene68486:CDS
TTGTCTAGATTCTCAATTGA+GGG 0.471983 3.2:+48503272 None:intergenic
TGCTTGTTGAAGCAGATTCA+AGG 0.473275 3.2:+48503180 None:intergenic
CTAATCTTTGGAGGTCCTCC+AGG 0.475160 3.2:+48502450 None:intergenic
TGAATGGTCACACTTTCATC+TGG 0.476627 3.2:+48502213 None:intergenic
TTCGATTGGACTGCCTGAAA+CGG 0.476643 3.2:+48502752 None:intergenic
TGGTACATGATGTAGCATAA+TGG 0.479324 3.2:+48502233 None:intergenic
AAATTTCTTACGGCTCTGAC+AGG 0.480661 3.2:-48503337 MS.gene68486:CDS
CAATTTGAAAATCAATTATG+TGG 0.486415 3.2:+48502520 None:intergenic
CTAACAGCATGGAACTCAAA+TGG 0.487453 3.2:+48502327 None:intergenic
TGTTCTAGAGTTTGATGAGA+AGG 0.490850 3.2:+48503212 None:intergenic
ATTCATCGTATGCCCGCGGT+GGG 0.494640 3.2:-48502404 MS.gene68486:CDS
GGTGAGAAACCGTGAGTAGA+GGG 0.496435 3.2:+48503131 None:intergenic
AAGTAAGAATCCTCCGCCTC+AGG 0.498080 3.2:+48503047 None:intergenic
ATCTCTAGCATCTCTACGTT+AGG 0.500658 3.2:+48502960 None:intergenic
ATGGTGCACTGTTTCTTGGT+TGG 0.503499 3.2:-48501792 MS.gene68486:CDS
CAATGGTTCACCTGAGGCGG+AGG 0.504414 3.2:-48503057 MS.gene68486:CDS
GTCACGAGCTTCTGAGTAAG+TGG 0.507829 3.2:-48501930 MS.gene68486:CDS
CAAAATTCTCCCTCTACTCA+CGG 0.508055 3.2:-48503140 MS.gene68486:CDS
TACGCTCCCAATCGTTCGAT+TGG 0.508907 3.2:+48502738 None:intergenic
TCCACTAGAGTCACCACCTT+AGG 0.511707 3.2:+48502147 None:intergenic
GGCAGTCCAATCGAACGATT+GGG 0.513943 3.2:-48502744 MS.gene68486:CDS
TCAGTGGGTGAGCCTAATCC+AGG 0.518047 3.2:-48502487 MS.gene68486:CDS
TGCACCATCTCTCTCTTCTA+GGG 0.519166 3.2:+48501807 None:intergenic
TCTTCGAGTCGCACCTCAGA+AGG 0.519582 3.2:+48502297 None:intergenic
TTCATCGTATGCCCGCGGTG+GGG 0.523230 3.2:-48502403 MS.gene68486:CDS
TTCTCACCTAACAATAGTCC+AGG 0.523328 3.2:-48503116 MS.gene68486:CDS
GTAGATTGTACTTCCAGAAG+AGG 0.526914 3.2:+48502686 None:intergenic
TTAAAATGCAGCGAGCCTAC+TGG 0.529023 3.2:-48502657 MS.gene68486:CDS
TCGACAAAACGCTGCAAGAA+CGG 0.530438 3.2:+48502099 None:intergenic
AATTGATGTTGTTCTTCCAA+GGG 0.531808 3.2:-48502043 MS.gene68486:CDS
ACATGGAGATGGTATCTAGA+GGG 0.537613 3.2:-48502872 MS.gene68486:CDS
TCTTATGCCTTAGGTCATCA+TGG 0.538147 3.2:+48503012 None:intergenic
ATTGTCTAGATTCTCAATTG+AGG 0.540884 3.2:+48503271 None:intergenic
TGTCAATGGTTCACCTGAGG+CGG 0.541775 3.2:-48503060 MS.gene68486:CDS
ATAGTCCAGGTTCTACGTTA+AGG 0.542222 3.2:-48503103 MS.gene68486:CDS
AGCCATCGCGGAAGCTATGA+AGG 0.542662 3.2:-48502556 MS.gene68486:CDS
TCGTGACAGGTTGCTGCGAT+TGG 0.544162 3.2:-48502184 MS.gene68486:CDS
GGCTAAGCACATGTCTCCTA+AGG 0.546864 3.2:-48502163 MS.gene68486:CDS
TAATCCAGGCTCTTGCTCGC+CGG 0.552248 3.2:-48502473 MS.gene68486:CDS
CTCCTTCATAGCTTCCGCGA+TGG 0.552554 3.2:+48502554 None:intergenic
CCATTCATAATCATCGTGAC+AGG 0.553760 3.2:-48502197 MS.gene68486:CDS
TGAACTTAGTAGCTTGCGAA+GGG 0.564647 3.2:-48501969 MS.gene68486:CDS
CCTGTCACGATGATTATGAA+TGG 0.565547 3.2:+48502197 None:intergenic
ATCATGACCATGATGACCTA+AGG 0.567502 3.2:-48503019 MS.gene68486:CDS
TTCATAAAGTACATGCATGT+AGG 0.567692 3.2:+48502620 None:intergenic
ACGATTGGGAGCGTACATGT+TGG 0.575556 3.2:-48502730 MS.gene68486:CDS
TCCTAAGGTGGTGACTCTAG+TGG 0.576537 3.2:-48502148 MS.gene68486:CDS
TATCGAGACAATTGAATGGA+TGG 0.577277 3.2:-48502799 MS.gene68486:CDS
AGGTGAGAAACCGTGAGTAG+AGG 0.577735 3.2:+48503130 None:intergenic
AAGGCATAAGATGAGTGAGC+TGG 0.578063 3.2:-48503000 MS.gene68486:CDS
ATGATATCGAGACAATTGAA+TGG 0.578703 3.2:-48502803 MS.gene68486:CDS
GTGAACTTAGTAGCTTGCGA+AGG 0.579196 3.2:-48501970 MS.gene68486:CDS
TCTTATTAGAGGCAGAGAAG+TGG 0.579922 3.2:-48502902 MS.gene68486:CDS
ATTGGGAGCGTACATGTTGG+AGG 0.587024 3.2:-48502727 MS.gene68486:CDS
TTATACTTGTGCAAAAGCTG+TGG 0.587474 3.2:-48502832 MS.gene68486:CDS
GCTCTTGCTCGCCGGCCTGG+AGG 0.588420 3.2:-48502465 MS.gene68486:CDS
TATTCATCGTATGCCCGCGG+TGG 0.589109 3.2:-48502405 MS.gene68486:CDS
TTCCAAGGGATCACAAAGAG+AGG 0.599204 3.2:-48502029 MS.gene68486:CDS
TGCGGCCTTAACGTAGAACC+TGG 0.605445 3.2:+48503098 None:intergenic
TACGACTCGAGATTCTGTAC+AGG 0.608834 3.2:+48503302 None:intergenic
TGTTAGAGTGTATCCTTCTG+AGG 0.611616 3.2:-48502310 MS.gene68486:CDS
AACATGGAGATGGTATCTAG+AGG 0.612916 3.2:-48502873 MS.gene68486:CDS
TCTTTGGAGGTCCTCCAGGC+CGG 0.613618 3.2:+48502454 None:intergenic
CTTCTAGGGTGTAATGTCCA+CGG 0.620198 3.2:+48501821 None:intergenic
AAGGATACACTCTAACAGCA+TGG 0.621994 3.2:+48502316 None:intergenic
GAATTGATGAAGGAGCTCAA+TGG 0.624450 3.2:+48501871 None:intergenic
AATCTTCTCGAGAGCTACCG+TGG 0.627998 3.2:-48501838 MS.gene68486:CDS
TCGTCCTAATGAAGCTGTGG+CGG 0.629870 3.2:-48502265 MS.gene68486:CDS
ATTTGTCAATGGTTCACCTG+AGG 0.630937 3.2:-48503063 MS.gene68486:CDS
ATATATCCGAATTTAAAGTA+CGG 0.633125 3.2:+48502591 None:intergenic
ATGACATGACTGCGCAAGCG+CGG 0.633313 3.2:+48502353 None:intergenic
ACAAATTCAAGTGAATGTTG+CGG 0.633662 3.2:+48503080 None:intergenic
CATGGAGATGGTATCTAGAG+GGG 0.636479 3.2:-48502871 MS.gene68486:CDS
TGTTTGGCAGCTGTTATCAG+AGG 0.643018 3.2:+48503241 None:intergenic
GAAAGCAGTTCATTACCAGT+AGG 0.643103 3.2:+48502642 None:intergenic
AATTGGACAAGGAACTCAGT+GGG 0.647751 3.2:-48502502 MS.gene68486:CDS
TAAGCACATGTCTCCTAAGG+TGG 0.649063 3.2:-48502160 MS.gene68486:CDS
ACTTCGTCCTAATGAAGCTG+TGG 0.652136 3.2:-48502268 MS.gene68486:CDS
TTGCGAAGGGGAAGAAAGAG+TGG 0.653404 3.2:-48501956 MS.gene68486:CDS
GTCTGCAAATGGAGCCATCG+CGG 0.659747 3.2:-48502568 MS.gene68486:CDS
GAGCTGGAATCTGTTATGCG+AGG 0.671920 3.2:-48502984 MS.gene68486:CDS
GAACTTAGTAGCTTGCGAAG+GGG 0.697810 3.2:-48501968 MS.gene68486:CDS
CATCGCGGAAGCTATGAAGG+AGG 0.707945 3.2:-48502553 MS.gene68486:CDS
TCTTATTCATCGTATGCCCG+CGG 0.710163 3.2:-48502408 MS.gene68486:CDS
TACACCCTAGAAGAGAGAGA+TGG 0.711736 3.2:-48501811 MS.gene68486:CDS
AAATTGGACAAGGAACTCAG+TGG 0.723091 3.2:-48502503 MS.gene68486:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTGAAAATCAATTATG+TGG + chr3.2:48502580-48502599 None:intergenic 20.0%
!!! AACATCAATTGATTCATAAA+CGG + chr3.2:48503044-48503063 None:intergenic 20.0%
!!! ATATATCCGAATTTAAAGTA+CGG + chr3.2:48502509-48502528 None:intergenic 20.0%
!!! CACATAATTGATTTTCAAAT+TGG - chr3.2:48502578-48502597 MS.gene68486:CDS 20.0%
! ATACCTGTTATTCTAATCTT+TGG + chr3.2:48502662-48502681 None:intergenic 25.0%
! ATATGTCCGTACTTTAAATT+CGG - chr3.2:48502500-48502519 MS.gene68486:CDS 25.0%
!!! ATTGATTTTCAAATTGGACA+AGG - chr3.2:48502584-48502603 MS.gene68486:CDS 25.0%
AATTGATGTTGTTCTTCCAA+GGG - chr3.2:48503054-48503073 MS.gene68486:CDS 30.0%
ACAAATTCAAGTGAATGTTG+CGG + chr3.2:48502020-48502039 None:intergenic 30.0%
ATGATATCGAGACAATTGAA+TGG - chr3.2:48502294-48502313 MS.gene68486:CDS 30.0%
ATTGTCTAGATTCTCAATTG+AGG + chr3.2:48501829-48501848 None:intergenic 30.0%
CATTCACTTGAATTTGTCAA+TGG - chr3.2:48502023-48502042 MS.gene68486:CDS 30.0%
CTTGATTGAAGAATTGATGA+AGG + chr3.2:48503239-48503258 None:intergenic 30.0%
TTCATAAAGTACATGCATGT+AGG + chr3.2:48502480-48502499 None:intergenic 30.0%
TTGTCTAGATTCTCAATTGA+GGG + chr3.2:48501828-48501847 None:intergenic 30.0%
! ATCTTATTGTTTCTTGTGCT+TGG - chr3.2:48503338-48503357 MS.gene68486:CDS 30.0%
AGAGAAGTGGAAGAAAAACA+TGG - chr3.2:48502208-48502227 MS.gene68486:CDS 35.0%
AGCAGATTCTTCTTGAACTT+TGG + chr3.2:48502171-48502190 None:intergenic 35.0%
ATCTGCTTCGTTCTTATTAG+AGG - chr3.2:48502184-48502203 MS.gene68486:CDS 35.0%
CAATTGATGTTGTTCTTCCA+AGG - chr3.2:48503053-48503072 MS.gene68486:CDS 35.0%
CCTCCAAAGATTAGAATAAC+AGG - chr3.2:48502656-48502675 MS.gene68486:CDS 35.0%
CCTGTTATTCTAATCTTTGG+AGG + chr3.2:48502659-48502678 None:intergenic 35.0%
GATGAGAAGGATAGAATGTT+TGG + chr3.2:48501875-48501894 None:intergenic 35.0%
TATCGAGACAATTGAATGGA+TGG - chr3.2:48502298-48502317 MS.gene68486:CDS 35.0%
TGGTACATGATGTAGCATAA+TGG + chr3.2:48502867-48502886 None:intergenic 35.0%
TTATACTTGTGCAAAAGCTG+TGG - chr3.2:48502265-48502284 MS.gene68486:CDS 35.0%
TTCGGATATATGTCTGCAAA+TGG - chr3.2:48502518-48502537 MS.gene68486:CDS 35.0%
!! GTAGAGGGAGAATTTTGATA+AGG + chr3.2:48501954-48501973 None:intergenic 35.0%
!! TGTTCTAGAGTTTGATGAGA+AGG + chr3.2:48501888-48501907 None:intergenic 35.0%
AAATTGGACAAGGAACTCAG+TGG - chr3.2:48502594-48502613 MS.gene68486:CDS 40.0%
AACATGGAGATGGTATCTAG+AGG - chr3.2:48502224-48502243 MS.gene68486:CDS 40.0%
AAGGATACACTCTAACAGCA+TGG + chr3.2:48502784-48502803 None:intergenic 40.0%
AATTGGACAAGGAACTCAGT+GGG - chr3.2:48502595-48502614 MS.gene68486:CDS 40.0%
ACATGGAGATGGTATCTAGA+GGG - chr3.2:48502225-48502244 MS.gene68486:CDS 40.0%
ATAGTCCAGGTTCTACGTTA+AGG - chr3.2:48501994-48502013 MS.gene68486:CDS 40.0%
ATCATGACCATGATGACCTA+AGG - chr3.2:48502078-48502097 MS.gene68486:CDS 40.0%
ATCTCTAGCATCTCTACGTT+AGG + chr3.2:48502140-48502159 None:intergenic 40.0%
ATGAAGGAGCTCAATGGATA+AGG + chr3.2:48503223-48503242 None:intergenic 40.0%
ATTTGTCAATGGTTCACCTG+AGG - chr3.2:48502034-48502053 MS.gene68486:CDS 40.0%
CAAAATTCTCCCTCTACTCA+CGG - chr3.2:48501957-48501976 MS.gene68486:CDS 40.0%
CCATTCATAATCATCGTGAC+AGG - chr3.2:48502900-48502919 MS.gene68486:CDS 40.0%
CCTGTCACGATGATTATGAA+TGG + chr3.2:48502903-48502922 None:intergenic 40.0%
CGCAAAATAGTATCCGTTTC+AGG - chr3.2:48502332-48502351 MS.gene68486:CDS 40.0%
CTAACAGCATGGAACTCAAA+TGG + chr3.2:48502773-48502792 None:intergenic 40.0%
GAAAGCAGTTCATTACCAGT+AGG + chr3.2:48502458-48502477 None:intergenic 40.0%
GAATTGATGAAGGAGCTCAA+TGG + chr3.2:48503229-48503248 None:intergenic 40.0%
GTAGAACCTGGACTATTGTT+AGG + chr3.2:48501990-48502009 None:intergenic 40.0%
GTAGATTGTACTTCCAGAAG+AGG + chr3.2:48502414-48502433 None:intergenic 40.0%
GTGGAAGAAAAACATGGAGA+TGG - chr3.2:48502214-48502233 MS.gene68486:CDS 40.0%
GTTTCAAAGATTGCCTCTTC+TGG - chr3.2:48502398-48502417 MS.gene68486:CDS 40.0%
TAATGTCGAACAGCACTGTT+TGG - chr3.2:48503093-48503112 MS.gene68486:CDS 40.0%
TCTTATGCCTTAGGTCATCA+TGG + chr3.2:48502088-48502107 None:intergenic 40.0%
TCTTATTAGAGGCAGAGAAG+TGG - chr3.2:48502195-48502214 MS.gene68486:CDS 40.0%
TGAATGGTCACACTTTCATC+TGG + chr3.2:48502887-48502906 None:intergenic 40.0%
TGTTAGAGTGTATCCTTCTG+AGG - chr3.2:48502787-48502806 MS.gene68486:CDS 40.0%
TTCTCACCTAACAATAGTCC+AGG - chr3.2:48501981-48502000 MS.gene68486:CDS 40.0%
! AAATTTCTTACGGCTCTGAC+AGG - chr3.2:48501760-48501779 MS.gene68486:CDS 40.0%
! TGAACTTAGTAGCTTGCGAA+GGG - chr3.2:48503128-48503147 MS.gene68486:CDS 40.0%
! TGCTTGTTGAAGCAGATTCA+AGG + chr3.2:48501920-48501939 None:intergenic 40.0%
AAGGCATAAGATGAGTGAGC+TGG - chr3.2:48502097-48502116 MS.gene68486:CDS 45.0%
ACTTCGTCCTAATGAAGCTG+TGG - chr3.2:48502829-48502848 MS.gene68486:CDS 45.0%
ATCCTCTCTTTGTGATCCCT+TGG + chr3.2:48503073-48503092 None:intergenic 45.0%
CATGGAGATGGTATCTAGAG+GGG - chr3.2:48502226-48502245 MS.gene68486:CDS 45.0%
GCTCACTCATCTTATGCCTT+AGG + chr3.2:48502097-48502116 None:intergenic 45.0%
TAAGCACATGTCTCCTAAGG+TGG - chr3.2:48502937-48502956 MS.gene68486:CDS 45.0%
TACACCCTAGAAGAGAGAGA+TGG - chr3.2:48503286-48503305 MS.gene68486:CDS 45.0%
TACGACTCGAGATTCTGTAC+AGG + chr3.2:48501798-48501817 None:intergenic 45.0%
TCGACAAAACGCTGCAAGAA+CGG + chr3.2:48503001-48503020 None:intergenic 45.0%
TCTTATTCATCGTATGCCCG+CGG - chr3.2:48502689-48502708 MS.gene68486:CDS 45.0%
TGCACCATCTCTCTCTTCTA+GGG + chr3.2:48503293-48503312 None:intergenic 45.0%
TGTTTGGCAGCTGTTATCAG+AGG + chr3.2:48501859-48501878 None:intergenic 45.0%
TTAAAATGCAGCGAGCCTAC+TGG - chr3.2:48502440-48502459 MS.gene68486:CDS 45.0%
TTCCAAGGGATCACAAAGAG+AGG - chr3.2:48503068-48503087 MS.gene68486:CDS 45.0%
TTCGATTGGACTGCCTGAAA+CGG + chr3.2:48502348-48502367 None:intergenic 45.0%
! AGAGATGGTGCACTGTTTCT+TGG - chr3.2:48503301-48503320 MS.gene68486:CDS 45.0%
! ATGGTGCACTGTTTCTTGGT+TGG - chr3.2:48503305-48503324 MS.gene68486:CDS 45.0%
! GAACTTAGTAGCTTGCGAAG+GGG - chr3.2:48503129-48503148 MS.gene68486:CDS 45.0%
! GTGAACTTAGTAGCTTGCGA+AGG - chr3.2:48503127-48503146 MS.gene68486:CDS 45.0%
!! CTTCTAGGGTGTAATGTCCA+CGG + chr3.2:48503279-48503298 None:intergenic 45.0%
!!! AAAAAGCGTTTTGCGATGGC+TGG - chr3.2:48503190-48503209 MS.gene68486:CDS 45.0%
!!! GTGGAAAAAGCGTTTTGCGA+TGG - chr3.2:48503186-48503205 MS.gene68486:CDS 45.0%
AAGTAAGAATCCTCCGCCTC+AGG + chr3.2:48502053-48502072 None:intergenic 50.0%
AATCTTCTCGAGAGCTACCG+TGG - chr3.2:48503259-48503278 MS.gene68486:CDS 50.0%
AGGCAGTCCAATCGAACGAT+TGG - chr3.2:48502352-48502371 MS.gene68486:CDS 50.0%
AGGTGAGAAACCGTGAGTAG+AGG + chr3.2:48501970-48501989 None:intergenic 50.0%
GAGCTGGAATCTGTTATGCG+AGG - chr3.2:48502113-48502132 MS.gene68486:CDS 50.0%
GGCAGTCCAATCGAACGATT+GGG - chr3.2:48502353-48502372 MS.gene68486:CDS 50.0%
GGCTAAGCACATGTCTCCTA+AGG - chr3.2:48502934-48502953 MS.gene68486:CDS 50.0%
GGTGAGAAACCGTGAGTAGA+GGG + chr3.2:48501969-48501988 None:intergenic 50.0%
GTCACGAGCTTCTGAGTAAG+TGG - chr3.2:48503167-48503186 MS.gene68486:CDS 50.0%
GTGCACCATCTCTCTCTTCT+AGG + chr3.2:48503294-48503313 None:intergenic 50.0%
TACGCTCCCAATCGTTCGAT+TGG + chr3.2:48502362-48502381 None:intergenic 50.0%
TCCACTAGAGTCACCACCTT+AGG + chr3.2:48502953-48502972 None:intergenic 50.0%
TCCTAAGGTGGTGACTCTAG+TGG - chr3.2:48502949-48502968 MS.gene68486:CDS 50.0%
TCGTCCTAATGAAGCTGTGG+CGG - chr3.2:48502832-48502851 MS.gene68486:CDS 50.0%
TGTCAATGGTTCACCTGAGG+CGG - chr3.2:48502037-48502056 MS.gene68486:CDS 50.0%
TTCACCGCCACAGCTTCATT+AGG + chr3.2:48502839-48502858 None:intergenic 50.0%
TTGCGAAGGGGAAGAAAGAG+TGG - chr3.2:48503141-48503160 MS.gene68486:CDS 50.0%
! ACGATTGGGAGCGTACATGT+TGG - chr3.2:48502367-48502386 MS.gene68486:CDS 50.0%
! ATTGGGAGCGTACATGTTGG+AGG - chr3.2:48502370-48502389 MS.gene68486:CDS 50.0%
! CTAATCTTTGGAGGTCCTCC+AGG + chr3.2:48502650-48502669 None:intergenic 50.0%
! CTGCAAGAACGGAGCGTTAT+TGG + chr3.2:48502990-48503009 None:intergenic 50.0%
!! GTGGGGGCGTTGATATAGTA+GGG - chr3.2:48502711-48502730 MS.gene68486:CDS 50.0%
!! TGGGGGCGTTGATATAGTAG+GGG - chr3.2:48502712-48502731 MS.gene68486:CDS 50.0%
AGCCATCGCGGAAGCTATGA+AGG - chr3.2:48502541-48502560 MS.gene68486:CDS 55.0%
ATGACATGACTGCGCAAGCG+CGG + chr3.2:48502747-48502766 None:intergenic 55.0%
ATTCATCGTATGCCCGCGGT+GGG - chr3.2:48502693-48502712 MS.gene68486:CDS 55.0%
CATCGCGGAAGCTATGAAGG+AGG - chr3.2:48502544-48502563 MS.gene68486:CDS 55.0%
CTCCTTCATAGCTTCCGCGA+TGG + chr3.2:48502546-48502565 None:intergenic 55.0%
GTCTGCAAATGGAGCCATCG+CGG - chr3.2:48502529-48502548 MS.gene68486:CDS 55.0%
TAATCCAGGCTCTTGCTCGC+CGG - chr3.2:48502624-48502643 MS.gene68486:CDS 55.0%
TATTCATCGTATGCCCGCGG+TGG - chr3.2:48502692-48502711 MS.gene68486:CDS 55.0%
TCAGTGGGTGAGCCTAATCC+AGG - chr3.2:48502610-48502629 MS.gene68486:CDS 55.0%
TCGTGACAGGTTGCTGCGAT+TGG - chr3.2:48502913-48502932 MS.gene68486:CDS 55.0%
TCTTCGAGTCGCACCTCAGA+AGG + chr3.2:48502803-48502822 None:intergenic 55.0%
TGCGGCCTTAACGTAGAACC+TGG + chr3.2:48502002-48502021 None:intergenic 55.0%
!! GGTGGGGGCGTTGATATAGT+AGG - chr3.2:48502710-48502729 MS.gene68486:CDS 55.0%
CAATGGTTCACCTGAGGCGG+AGG - chr3.2:48502040-48502059 MS.gene68486:CDS 60.0%
CTATATCAACGCCCCCACCG+CGG + chr3.2:48502709-48502728 None:intergenic 60.0%
GGCGAGCAAGAGCCTGGATT+AGG + chr3.2:48502625-48502644 None:intergenic 60.0%
TATATCAACGCCCCCACCGC+GGG + chr3.2:48502708-48502727 None:intergenic 60.0%
TTCATCGTATGCCCGCGGTG+GGG - chr3.2:48502694-48502713 MS.gene68486:CDS 60.0%
! TCTTTGGAGGTCCTCCAGGC+CGG + chr3.2:48502646-48502665 None:intergenic 60.0%
TCATCGTATGCCCGCGGTGG+GGG - chr3.2:48502695-48502714 MS.gene68486:CDS 65.0%
CAGGCCGGCGAGCAAGAGCC+TGG + chr3.2:48502631-48502650 None:intergenic 75.0%
CAGGCTCTTGCTCGCCGGCC+TGG - chr3.2:48502629-48502648 MS.gene68486:CDS 75.0%
GCTCTTGCTCGCCGGCCTGG+AGG - chr3.2:48502632-48502651 MS.gene68486:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 48501753 48503366 48501753 ID=MS.gene68486
chr3.2 mRNA 48501753 48503366 48501753 ID=MS.gene68486.t1;Parent=MS.gene68486
chr3.2 exon 48501753 48503366 48501753 ID=MS.gene68486.t1.exon1;Parent=MS.gene68486.t1
chr3.2 CDS 48501753 48503366 48501753 ID=cds.MS.gene68486.t1;Parent=MS.gene68486.t1
Gene Sequence

>MS.gene68486

AAACATGAAATTTCTTACGGCTCTGACAGGTTCTACGTTGAGCCTGTACAGAATCTCGAGTCGTATTGCTTACCCTCAATTGAGAATCTAGACAATTATTCTTCCTCTGATAACAGCTGCCAAACATTCTATCCTTCTCATCAAACTCTAGAACAATACAACAACCTTGAATCTGCTTCAACAAGCAACAACAGTTTACCTTATCAAAATTCTCCCTCTACTCACGGTTTCTCACCTAACAATAGTCCAGGTTCTACGTTAAGGCCGCAACATTCACTTGAATTTGTCAATGGTTCACCTGAGGCGGAGGATTCTTACTTGACATATCATGACCATGATGACCTAAGGCATAAGATGAGTGAGCTGGAATCTGTTATGCGAGGACCTAACGTAGAGATGCTAGAGATGTATGATACCAAAGTTCAAGAAGAATCTGCTTCGTTCTTATTAGAGGCAGAGAAGTGGAAGAAAAACATGGAGATGGTATCTAGAGGGGATTTGAAAGAGATGCTTTATACTTGTGCAAAAGCTGTGGATGTGAATGATATCGAGACAATTGAATGGATGGTAACTGAGTTACGCAAAATAGTATCCGTTTCAGGCAGTCCAATCGAACGATTGGGAGCGTACATGTTGGAGGCACTTGTTTCAAAGATTGCCTCTTCTGGAAGTACAATCTACAAATCGTTAAAATGCAGCGAGCCTACTGGTAATGAACTGCTTTCCTACATGCATGTACTTTATGAAATATGTCCGTACTTTAAATTCGGATATATGTCTGCAAATGGAGCCATCGCGGAAGCTATGAAGGAGGAAAATGAAGTCCACATAATTGATTTTCAAATTGGACAAGGAACTCAGTGGGTGAGCCTAATCCAGGCTCTTGCTCGCCGGCCTGGAGGACCTCCAAAGATTAGAATAACAGGTATTGATGACTCTTATTCATCGTATGCCCGCGGTGGGGGCGTTGATATAGTAGGGGAGAAGTTATCCGCGCTTGCGCAGTCATGTCATGTACCATTTGAGTTCCATGCTGTTAGAGTGTATCCTTCTGAGGTGCGACTCGAAGACTTTGAACTTCGTCCTAATGAAGCTGTGGCGGTGAACTTTGCCATTATGCTACATCATGTACCAGATGAAAGTGTGACCATTCATAATCATCGTGACAGGTTGCTGCGATTGGCTAAGCACATGTCTCCTAAGGTGGTGACTCTAGTGGAGCAAGAATTCAACACCAATAACGCTCCGTTCTTGCAGCGTTTTGTCGAGACAATGAACTACTACTCAGCCGTTTATGAATCAATTGATGTTGTTCTTCCAAGGGATCACAAAGAGAGGATTAATGTCGAACAGCACTGTTTGGCTCGTGAAGTTGTGAACTTAGTAGCTTGCGAAGGGGAAGAAAGAGTGGAACGTCACGAGCTTCTGAGTAAGTGGAAAAAGCGTTTTGCGATGGCTGGATTTACGCCTTATCCATTGAGCTCCTTCATCAATTCTTCAATCAAGAATCTTCTCGAGAGCTACCGTGGACATTACACCCTAGAAGAGAGAGATGGTGCACTGTTTCTTGGTTGGATGAATCAAGATCTTATTGTTTCTTGTGCTTGGAGATGA
Protein sequence

>MS.gene68486.t1

KHEISYGSDRFYVEPVQNLESYCLPSIENLDNYSSSDNSCQTFYPSHQTLEQYNNLESASTSNNSLPYQNSPSTHGFSPNNSPGSTLRPQHSLEFVNGSPEAEDSYLTYHDHDDLRHKMSELESVMRGPNVEMLEMYDTKVQEESASFLLEAEKWKKNMEMVSRGDLKEMLYTCAKAVDVNDIETIEWMVTELRKIVSVSGSPIERLGAYMLEALVSKIASSGSTIYKSLKCSEPTGNELLSYMHVLYEICPYFKFGYMSANGAIAEAMKEENEVHIIDFQIGQGTQWVSLIQALARRPGGPPKIRITGIDDSYSSYARGGGVDIVGEKLSALAQSCHVPFEFHAVRVYPSEVRLEDFELRPNEAVAVNFAIMLHHVPDESVTIHNHRDRLLRLAKHMSPKVVTLVEQEFNTNNAPFLQRFVETMNYYSAVYESIDVVLPRDHKERINVEQHCLAREVVNLVACEGEERVERHELLSKWKKRFAMAGFTPYPLSSFINSSIKNLLESYRGHYTLEERDGALFLGWMNQDLIVSCAWR