Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68593.t1 | XP_013465039.1 | 98.1 | 107 | 2 | 0 | 1 | 107 | 73 | 179 | 1.40E-57 | 231.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68593.t1 | Q8GZA8 | 70.1 | 107 | 32 | 0 | 1 | 107 | 74 | 180 | 3.8e-43 | 175.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68593.t1 | A0A072VAL1 | 98.1 | 107 | 2 | 0 | 1 | 107 | 73 | 179 | 1.0e-57 | 231.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene68593.t1 | TF | ULT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68593.t1 | MTR_2g090080 | 98.131 | 107 | 2 | 0 | 1 | 107 | 73 | 179 | 1.05e-75 | 223 |
| MS.gene68593.t1 | MTR_0072s0040 | 79.439 | 107 | 22 | 0 | 1 | 107 | 76 | 182 | 1.75e-60 | 185 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68593.t1 | AT4G28190 | 70.093 | 107 | 32 | 0 | 1 | 107 | 74 | 180 | 1.32e-54 | 170 |
| MS.gene68593.t1 | AT4G28190 | 70.093 | 107 | 32 | 0 | 1 | 107 | 105 | 211 | 1.43e-54 | 171 |
| MS.gene68593.t1 | AT2G20825 | 65.421 | 107 | 31 | 1 | 1 | 107 | 70 | 170 | 1.12e-47 | 152 |
Find 0 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACTTGTCATATGGAAGAT+CGG | - | 15407:19070-19089 | MS.gene68593:intergenic | 30.0% | |
| GAAATGGAAGAACAATATCT+GGG | + | 15407:18813-18832 | MS.gene68593:CDS | 30.0% | |
| ! | AGCAGAACTGTTTTATACAA+TGG | - | 15407:18862-18881 | MS.gene68593:intergenic | 30.0% |
| ! | GCAGAACTGTTTTATACAAT+GGG | - | 15407:18861-18880 | MS.gene68593:intergenic | 30.0% |
| AATATCTGGGTAACAGTTGA+TGG | + | 15407:18826-18845 | MS.gene68593:CDS | 35.0% | |
| AGATCGGAACATTTCCAATT+TGG | - | 15407:19054-19073 | MS.gene68593:intergenic | 35.0% | |
| ATGCTTTAGCTGATCCAAAT+TGG | + | 15407:19037-19056 | MS.gene68593:CDS | 35.0% | |
| CAAGCATTAAAAACAGCCAA+TGG | + | 15407:18895-18914 | MS.gene68593:CDS | 35.0% | |
| CTGTCCATTATTGGATTTGT+GGG | - | 15407:18923-18942 | MS.gene68593:intergenic | 35.0% | |
| GGAAATGGAAGAACAATATC+TGG | + | 15407:18812-18831 | MS.gene68593:CDS | 35.0% | |
| ATTGGATTTGTGGGAACCAT+TGG | - | 15407:18914-18933 | MS.gene68593:intergenic | 40.0% | |
| CCTGTCCATTATTGGATTTG+TGG | - | 15407:18924-18943 | MS.gene68593:intergenic | 40.0% | |
| GAAGAGAAACAGCTAGGAAA+TGG | + | 15407:18797-18816 | MS.gene68593:CDS | 40.0% | |
| GGTTCCCACAAATCCAATAA+TGG | + | 15407:18916-18935 | MS.gene68593:CDS | 40.0% | |
| TACAGCAGATAAACTCGTCA+CGG | - | 15407:18954-18973 | MS.gene68593:intergenic | 40.0% | |
| ! | ATTCTGGAAGAGAAACAGCT+AGG | + | 15407:18791-18810 | MS.gene68593:CDS | 40.0% |
| ! | TTGGATCAGCTAAAGCATCA+TGG | - | 15407:19035-19054 | MS.gene68593:intergenic | 40.0% |
| !! | ATTCCTCTTTAGTTCTCAGG+CGG | - | 15407:19002-19021 | MS.gene68593:intergenic | 40.0% |
| !! | CCACAAATCCAATAATGGAC+AGG | + | 15407:18921-18940 | MS.gene68593:CDS | 40.0% |
| !! | GACATTCCTCTTTAGTTCTC+AGG | - | 15407:19005-19024 | MS.gene68593:intergenic | 40.0% |
| ! | CAGCTAAAGCATCATGGTGA+CGG | - | 15407:19029-19048 | MS.gene68593:intergenic | 45.0% |
| ! | CTAGATGTAACAAAGAGCGC+AGG | + | 15407:18974-18993 | MS.gene68593:CDS | 45.0% |
| AGCAGATAAACTCGTCACGG+TGG | - | 15407:18951-18970 | MS.gene68593:intergenic | 50.0% | |
| GAATGTTTCTCGAACGCAGC+AGG | - | 15407:18775-18794 | MS.gene68593:intergenic | 50.0% | |
| GCTGCGTTCGAGAAACATTC+TGG | + | 15407:18775-18794 | MS.gene68593:CDS | 50.0% | |
| GTTCCGCCTGAGAACTAAAG+AGG | + | 15407:18996-19015 | MS.gene68593:CDS | 50.0% | |
| !! | GGTAACAGTTGATGGCGAGA+AGG | + | 15407:18834-18853 | MS.gene68593:CDS | 50.0% |
| GTGGCACGCCTGTCCATTAT+TGG | - | 15407:18932-18951 | MS.gene68593:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15407 | gene | 18766 | 19089 | 18766 | ID=MS.gene68593 |
| 15407 | mRNA | 18766 | 19089 | 18766 | ID=MS.gene68593.t1;Parent=MS.gene68593 |
| 15407 | exon | 18766 | 19089 | 18766 | ID=MS.gene68593.t1.exon1;Parent=MS.gene68593.t1 |
| 15407 | CDS | 18766 | 19089 | 18766 | ID=cds.MS.gene68593.t1;Parent=MS.gene68593.t1 |
| Gene Sequence |
| Protein sequence |
