AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68750.t1 MTR_3g116690 81.400 457 64 8 1 456 1 437 0.0 677
MS.gene68750.t1 MTR_3g117790 81.400 457 64 8 1 456 1 437 0.0 669
MS.gene68750.t1 MTR_3g117790 79.650 457 63 9 1 456 1 428 0.0 645
MS.gene68750.t1 MTR_3g014420 79.839 124 20 2 194 317 17 135 6.43e-60 192
MS.gene68750.t1 MTR_3g467090 32.443 262 120 5 220 452 387 620 2.81e-29 121
MS.gene68750.t1 MTR_3g467090 34.135 208 108 9 6 209 8 190 1.88e-17 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68750.t1 AT5G58340 25.984 381 247 13 89 450 81 445 1.43e-14 75.9
MS.gene68750.t1 AT5G58340 25.984 381 247 13 89 450 81 445 1.43e-14 75.9

Find 110 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATCATTGTTAACATTT+AGG 0.186976 3.2:-92969161 None:intergenic
AAAGAAATAACCAACCATTA+TGG 0.218651 3.2:-92968343 None:intergenic
CTGCTTGGAGGTAGTGTTTA+GGG 0.228026 3.2:-92968061 None:intergenic
CTCTTGAATGGCTTTCCTTC+TGG 0.254243 3.2:-92968228 None:intergenic
TCCAAGCAGTTGACAGAATA+TGG 0.254939 3.2:+92968075 MS.gene68750:CDS
TAAGTGAATCCCCGTTCTTT+TGG 0.273307 3.2:-92968975 None:intergenic
TAACTCCAAAATTGAGATTC+TGG 0.273343 3.2:+92968138 MS.gene68750:CDS
GTCTGGCATACAAATAGAAT+TGG 0.288931 3.2:-92969043 None:intergenic
GCATCGCTAATAGGTGGATT+AGG 0.298127 3.2:-92967957 None:intergenic
AAAGACAATGGAAGGCTTAA+AGG 0.299896 3.2:+92968865 MS.gene68750:CDS
TGAGTAAGAGCAGAACTATT+TGG 0.309160 3.2:-92969243 None:intergenic
ACTGCTTGGAGGTAGTGTTT+AGG 0.310482 3.2:-92968062 None:intergenic
TATAGAGAGTGAGCATATTA+AGG 0.313329 3.2:+92967758 MS.gene68750:CDS
TCAGAATTGGAGAAGGAAAC+TGG 0.354877 3.2:+92969951 MS.gene68750:intron
AAGAAATAACCAACCATTAT+GGG 0.366017 3.2:-92968342 None:intergenic
CATCTTCCGATTATCGATTT+CGG 0.378562 3.2:-92967808 None:intergenic
GATGATGAACATCACTTTGA+TGG 0.379549 3.2:-92969206 None:intergenic
CATCGCTAATAGGTGGATTA+GGG 0.381491 3.2:-92967956 None:intergenic
CCATTCATCGATAGATGCTT+TGG 0.387362 3.2:-92967871 None:intergenic
TGTTGTTGTTGTTGAGGAAA+TGG 0.391023 3.2:+92968903 MS.gene68750:CDS
GATTTCATGGCATCGCTAAT+AGG 0.391784 3.2:-92967966 None:intergenic
GTAGACATAAGCAGATTTCA+TGG 0.396640 3.2:-92967979 None:intergenic
GGAAAGGTTAGGGTGGTGTT+TGG 0.397081 3.2:+92967707 MS.gene68750:CDS
TTTGTATGCCAGACATTGTA+AGG 0.401380 3.2:+92969052 MS.gene68750:CDS
GAAGAAGCTTTCTCCGTTGA+AGG 0.405658 3.2:+92969496 MS.gene68750:CDS
CACCTGCACCTGCTGCTTCA+AGG 0.406135 3.2:-92968028 None:intergenic
GGAAGTTGCCTTCGTAAGAT+TGG 0.411891 3.2:-92969184 None:intergenic
TGGTGTTTGGAATTCGTTCT+TGG 0.411975 3.2:+92967720 MS.gene68750:CDS
GCTCAAAATCAATCCCATAA+TGG 0.413594 3.2:+92968329 MS.gene68750:CDS
TTAACGAGGCCCAAAAGAAC+GGG 0.420375 3.2:+92968965 MS.gene68750:CDS
TCCATATTCTGTCAACTGCT+TGG 0.421745 3.2:-92968076 None:intergenic
GGTGGATTAGGGTTTGAAGA+AGG 0.424767 3.2:-92967945 None:intergenic
TTTAGGCAAGCATGTGGGTT+GGG 0.424815 3.2:-92969144 None:intergenic
AAAACTACTTCTAATTGATT+GGG 0.433610 3.2:+92967920 MS.gene68750:CDS
GCATGTATCGTGAGTTGAAA+AGG 0.433870 3.2:-92968181 None:intergenic
GCAAATAACTTCTTCCCTCC+AGG 0.443932 3.2:-92968115 None:intergenic
TGGACAACAACTCATCTTCA+AGG 0.456297 3.2:-92967851 None:intergenic
AATGGGAAGAGGATAATCTA+AGG 0.459156 3.2:+92969570 MS.gene68750:CDS
GGAGAAAGCTTCTTCCTCTC+TGG 0.461592 3.2:-92969488 None:intergenic
CACAAATCAGGAGCCACAAA+TGG 0.462731 3.2:+92969095 MS.gene68750:CDS
TGGAGAAATTTGAAGCGGTC+TGG 0.467731 3.2:+92970109 MS.gene68750:CDS
ATTTGAAAATGGAAAGGTTA+GGG 0.470688 3.2:+92967697 None:intergenic
ACATCAGACCTTACAATGTC+TGG 0.471043 3.2:-92969060 None:intergenic
GTACATTTGCATCTTTACTC+TGG 0.471288 3.2:-92969122 None:intergenic
AATTTGAAAATGGAAAGGTT+AGG 0.473595 3.2:+92967696 None:intergenic
TGATTCGAACGATGGATTGC+AGG 0.474019 3.2:+92969424 MS.gene68750:CDS
AAGAACATACAGTTTAATTC+CGG 0.477098 3.2:-92969594 None:intergenic
GAAGATGAAAATGCTTCTGT+TGG 0.479184 3.2:+92967824 MS.gene68750:CDS
TACCTTGAAGCAGCAGGTGC+AGG 0.480680 3.2:+92968026 MS.gene68750:CDS
GCAACAGAGTGAAGAAAGGA+AGG 0.481758 3.2:-92968293 None:intergenic
GGCAATTGAAGACACAAATC+AGG 0.483604 3.2:+92969083 MS.gene68750:CDS
CAGTCACCGAAATCGATAAT+CGG 0.485524 3.2:+92967802 MS.gene68750:CDS
GATGCTCGGAATAACCTTGT+AGG 0.496320 3.2:+92968269 MS.gene68750:CDS
TCTGGCATACAAATAGAATT+GGG 0.497185 3.2:-92969042 None:intergenic
TACTACTGTTGTTGTTGTTG+AGG 0.498274 3.2:+92968897 MS.gene68750:CDS
ATATTCTGTCAACTGCTTGG+AGG 0.502799 3.2:-92968073 None:intergenic
CTATTTGGTTTCATCAAGCT+AGG 0.506498 3.2:-92969228 None:intergenic
ATGAAAATGCTTCTGTTGGA+AGG 0.511456 3.2:+92967828 MS.gene68750:CDS
CCAAAGCATCTATCGATGAA+TGG 0.516478 3.2:+92967871 MS.gene68750:CDS
TGAAAAGGGAAGCATCGATT+CGG 0.518415 3.2:-92968166 None:intergenic
TTGTCTTTGTCGAGTTCCAT+CGG 0.525502 3.2:-92968850 None:intergenic
GTGGATTAGGGTTTGAAGAA+GGG 0.529616 3.2:-92967944 None:intergenic
AGCAGCAACAGAGTGAAGAA+AGG 0.532298 3.2:-92968297 None:intergenic
CATGTATCGTGAGTTGAAAA+GGG 0.536326 3.2:-92968180 None:intergenic
GATTGCAGGCAGGAACATCA+AGG 0.543132 3.2:+92969438 MS.gene68750:CDS
TTCACTTAAATGCAATTCTG+TGG 0.545122 3.2:+92968990 MS.gene68750:CDS
TTTGGTTCATACTCCTTCAA+CGG 0.551515 3.2:-92969509 None:intergenic
AAAATGGAGTCAATGGGAAG+AGG 0.556320 3.2:+92969559 MS.gene68750:CDS
ATTTAGGCAAGCATGTGGGT+TGG 0.556821 3.2:-92969145 None:intergenic
AAAGGAATTTGAAAATGGAA+AGG 0.559315 3.2:+92967691 None:intergenic
GAACCGTAGGTTTCAAAACC+TGG 0.560313 3.2:+92968097 MS.gene68750:CDS
ATGATGAACATCACTTTGAT+GGG 0.562151 3.2:-92969205 None:intergenic
TCGAACGATGGATTGCAGGC+AGG 0.564385 3.2:+92969428 MS.gene68750:CDS
GAAGAGGATAATCTAAGGAC+CGG 0.564580 3.2:+92969575 MS.gene68750:CDS
GTTAACGAGGCCCAAAAGAA+CGG 0.567267 3.2:+92968964 MS.gene68750:CDS
GAAGAAAGGAAGGGCCTACA+AGG 0.570430 3.2:-92968283 None:intergenic
CTTCATTGTCTGATACCAGA+AGG 0.585689 3.2:+92968213 MS.gene68750:CDS
AACTCGACAAAGACAATGGA+AGG 0.587275 3.2:+92968857 MS.gene68750:CDS
CAATGGAAGGCTTAAAGGCA+AGG 0.588132 3.2:+92968870 MS.gene68750:CDS
TTGCTGTGGAATGTACCGTT+AGG 0.589094 3.2:+92968003 MS.gene68750:CDS
TTCATGGCATCGCTAATAGG+TGG 0.591268 3.2:-92967963 None:intergenic
AGGTTGATTTGAAAGACAAG+TGG 0.593616 3.2:+92970089 MS.gene68750:intron
TAGTGGGATGATTCGAACGA+TGG 0.595110 3.2:+92969416 MS.gene68750:intron
TTGACAGAATATGGAACCGT+AGG 0.599653 3.2:+92968084 MS.gene68750:CDS
TGACTCTTTGCCAGTTAACG+AGG 0.601690 3.2:+92968951 MS.gene68750:CDS
TGCTTATGTCTACATTGCTG+TGG 0.602167 3.2:+92967989 MS.gene68750:CDS
TTAACATTTAGGCAAGCATG+TGG 0.604145 3.2:-92969150 None:intergenic
CTTGAAGCAGCAGGTGCAGG+TGG 0.604795 3.2:+92968029 MS.gene68750:CDS
TCTCATATTAGAGTCTCCGA+TGG 0.606409 3.2:+92968834 MS.gene68750:intron
ATTGCAGGCAGGAACATCAA+GGG 0.616311 3.2:+92969439 MS.gene68750:CDS
GTTAGGTACCTTGAAGCAGC+AGG 0.617125 3.2:+92968020 MS.gene68750:CDS
ATTATCGATTTCGGTGACTG+AGG 0.617150 3.2:-92967799 None:intergenic
ATGGAACTCGACAAAGACAA+TGG 0.617169 3.2:+92968853 MS.gene68750:CDS
TGAAAATGGAAAGGTTAGGG+TGG 0.628807 3.2:+92967700 None:intergenic
CCTAAATGTTAACAATGATG+AGG 0.630277 3.2:+92969161 MS.gene68750:CDS
TGCTGCTTCAAGGTACCTAA+CGG 0.634086 3.2:-92968018 None:intergenic
ATACAAATAGAATTGGGCAC+AGG 0.635848 3.2:-92969036 None:intergenic
ATGGATGCTTGAACTTCTCG+AGG 0.640307 3.2:+92967890 MS.gene68750:CDS
TTGCAGGCAGGAACATCAAG+GGG 0.642905 3.2:+92969440 MS.gene68750:CDS
TTCGTTTGCGTAGTCCAGAG+AGG 0.651598 3.2:+92969474 MS.gene68750:CDS
CAACAGAGTGAAGAAAGGAA+GGG 0.656227 3.2:-92968292 None:intergenic
CGTAGGTTTCAAAACCTGGA+GGG 0.661614 3.2:+92968101 MS.gene68750:CDS
CAGAAGGAAAGCCATTCAAG+AGG 0.668057 3.2:+92968229 MS.gene68750:CDS
TAACATTTAGGCAAGCATGT+GGG 0.675244 3.2:-92969149 None:intergenic
ACAAGTGGAGAAATTTGAAG+CGG 0.678726 3.2:+92970104 MS.gene68750:CDS
AGACATTGTAAGGTCTGATG+TGG 0.687030 3.2:+92969062 MS.gene68750:CDS
CCGTAGGTTTCAAAACCTGG+AGG 0.690526 3.2:+92968100 MS.gene68750:CDS
TGATGAGGCCAATCTTACGA+AGG 0.699645 3.2:+92969176 MS.gene68750:CDS
TAGTTCTGCTCTTACTCACG+AGG 0.704886 3.2:+92969248 MS.gene68750:CDS
TAACGAGGCCCAAAAGAACG+GGG 0.712659 3.2:+92968966 MS.gene68750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTTGAATAGTAAAA+TGG - chr3.2:92969828-92969847 None:intergenic 10.0%
!! AAATATTATAGTAACTTGAT+TGG - chr3.2:92969627-92969646 None:intergenic 15.0%
!! TAAAATAAGCAATAAAATCA+AGG - chr3.2:92969394-92969413 None:intergenic 15.0%
!!! TTTGTAATTTTTTGTATTTC+TGG + chr3.2:92969280-92969299 MS.gene68750:intron 15.0%
!! AAAAACTACTTCTAATTGAT+TGG + chr3.2:92967919-92967938 MS.gene68750:CDS 20.0%
!! AAAAATGTATGACAAAGTAA+AGG - chr3.2:92969717-92969736 None:intergenic 20.0%
!! AAAACTACTTCTAATTGATT+GGG + chr3.2:92967920-92967939 MS.gene68750:CDS 20.0%
!! AAAGGAGAAAGAAAAAAAAA+TGG + chr3.2:92969543-92969562 MS.gene68750:CDS 20.0%
!! AACCAAAAAATATTACGAAA+AGG + chr3.2:92969525-92969544 MS.gene68750:CDS 20.0%
!! AATTGAAGCATAAATAGTTA+GGG - chr3.2:92969853-92969872 None:intergenic 20.0%
!!! ATACAATGTTTTTTCGTTTT+TGG + chr3.2:92969664-92969683 MS.gene68750:intron 20.0%
!!! CTTTTTTCTTTTTTCAGAAT+TGG + chr3.2:92969938-92969957 MS.gene68750:intron 20.0%
!!! TATTGCTTATTTTATGTAGT+GGG + chr3.2:92969400-92969419 MS.gene68750:intron 20.0%
!!! TGAATCAAATTTTGTTTATC+AGG + chr3.2:92970069-92970088 MS.gene68750:intron 20.0%
!!! TTATTGCTTATTTTATGTAG+TGG + chr3.2:92969399-92969418 MS.gene68750:intron 20.0%
! AAAGAAATAACCAACCATTA+TGG - chr3.2:92968346-92968365 None:intergenic 25.0%
! AAAGTAAAGGTAAAAGCAAA+AGG - chr3.2:92969704-92969723 None:intergenic 25.0%
! AACAACAACAACAGAAAAAA+GGG - chr3.2:92968371-92968390 None:intergenic 25.0%
! AAGAAATAACCAACCATTAT+GGG - chr3.2:92968345-92968364 None:intergenic 25.0%
! AAGAACATACAGTTTAATTC+CGG - chr3.2:92969597-92969616 None:intergenic 25.0%
! AATTTCATTGCAAAATGCAT+AGG + chr3.2:92968665-92968684 MS.gene68750:intron 25.0%
! AGAAAAAAAAATGGAGTCAA+TGG + chr3.2:92969552-92969571 MS.gene68750:CDS 25.0%
! AGAACAAAAAATTACAAGCA+TGG - chr3.2:92969364-92969383 None:intergenic 25.0%
! AGAGAGAGATATATATAAAG+CGG - chr3.2:92968405-92968424 None:intergenic 25.0%
! ATTTCATTGCAAAATGCATA+GGG + chr3.2:92968666-92968685 MS.gene68750:intron 25.0%
! CAATTGAAGCATAAATAGTT+AGG - chr3.2:92969854-92969873 None:intergenic 25.0%
! GAAAAAAAAATGGAGTCAAT+GGG + chr3.2:92969553-92969572 MS.gene68750:CDS 25.0%
!! CTCCTTTTCGTAATATTTTT+TGG - chr3.2:92969530-92969549 None:intergenic 25.0%
!!! AAAAGTTCTAAAAAGCAAAC+AGG - chr3.2:92968792-92968811 None:intergenic 25.0%
!!! CTAGTGTTTTTAGAGTAAAA+AGG - chr3.2:92969312-92969331 None:intergenic 25.0%
AAAAAGTTGAACCTCTTGAA+TGG - chr3.2:92968243-92968262 None:intergenic 30.0%
AAAATCAATCCCATAATGGT+TGG + chr3.2:92968333-92968352 MS.gene68750:intron 30.0%
AACTGAAGTAAGTCTAGTAA+AGG + chr3.2:92970009-92970028 MS.gene68750:intron 30.0%
AAGCAATAAAATCAAGGCAT+CGG - chr3.2:92969388-92969407 None:intergenic 30.0%
ACAACAACAACAGAAAAAAG+GGG - chr3.2:92968370-92968389 None:intergenic 30.0%
ATGATGAACATCACTTTGAT+GGG - chr3.2:92969208-92969227 None:intergenic 30.0%
CAACAACAACAACAGAAAAA+AGG - chr3.2:92968372-92968391 None:intergenic 30.0%
CCTAAATGTTAACAATGATG+AGG + chr3.2:92969161-92969180 MS.gene68750:CDS 30.0%
CCTCATCATTGTTAACATTT+AGG - chr3.2:92969164-92969183 None:intergenic 30.0%
TAACTCCAAAATTGAGATTC+TGG + chr3.2:92968138-92968157 MS.gene68750:CDS 30.0%
TATAGAGAGTGAGCATATTA+AGG + chr3.2:92967758-92967777 MS.gene68750:CDS 30.0%
TCTGGCATACAAATAGAATT+GGG - chr3.2:92969045-92969064 None:intergenic 30.0%
TTCACTTAAATGCAATTCTG+TGG + chr3.2:92968990-92969009 MS.gene68750:CDS 30.0%
! AGTAAGTCTAGTAAAGGTAT+TGG + chr3.2:92970015-92970034 MS.gene68750:intron 30.0%
! CTTTTCCAGAATCTCAATTT+TGG - chr3.2:92968146-92968165 None:intergenic 30.0%
!!! TCAAGAGGTTCAACTTTTTT+TGG + chr3.2:92968244-92968263 MS.gene68750:CDS 30.0%
!!! TCTTTTTTCAGAATTGGAGA+AGG + chr3.2:92969944-92969963 MS.gene68750:intron 30.0%
AAAGACAATGGAAGGCTTAA+AGG + chr3.2:92968865-92968884 MS.gene68750:CDS 35.0%
AATGGGAAGAGGATAATCTA+AGG + chr3.2:92969570-92969589 MS.gene68750:CDS 35.0%
ACAAGTGGAGAAATTTGAAG+CGG + chr3.2:92970104-92970123 MS.gene68750:CDS 35.0%
ATACAAATAGAATTGGGCAC+AGG - chr3.2:92969039-92969058 None:intergenic 35.0%
ATGAAAATGCTTCTGTTGGA+AGG + chr3.2:92967828-92967847 MS.gene68750:CDS 35.0%
ATGAAGCACAAGCACATATA+CGG - chr3.2:92968564-92968583 None:intergenic 35.0%
CCTAACCAATGAATGATTCA+TGG - chr3.2:92968695-92968714 None:intergenic 35.0%
GAAGATGAAAATGCTTCTGT+TGG + chr3.2:92967824-92967843 MS.gene68750:CDS 35.0%
GATGATGAACATCACTTTGA+TGG - chr3.2:92969209-92969228 None:intergenic 35.0%
GCTCAAAATCAATCCCATAA+TGG + chr3.2:92968329-92968348 MS.gene68750:CDS 35.0%
GTACATTTGCATCTTTACTC+TGG - chr3.2:92969125-92969144 None:intergenic 35.0%
GTAGACATAAGCAGATTTCA+TGG - chr3.2:92967982-92968001 None:intergenic 35.0%
GTCTGGCATACAAATAGAAT+TGG - chr3.2:92969046-92969065 None:intergenic 35.0%
TAACATTTAGGCAAGCATGT+GGG - chr3.2:92969152-92969171 None:intergenic 35.0%
TATATGTGCTTGTGCTTCAT+AGG + chr3.2:92968564-92968583 MS.gene68750:intron 35.0%
TCTCTCTCCCAAAGTATATA+AGG - chr3.2:92968646-92968665 None:intergenic 35.0%
TGAGTAAGAGCAGAACTATT+TGG - chr3.2:92969246-92969265 None:intergenic 35.0%
TTAACATTTAGGCAAGCATG+TGG - chr3.2:92969153-92969172 None:intergenic 35.0%
TTTGTATGCCAGACATTGTA+AGG + chr3.2:92969052-92969071 MS.gene68750:CDS 35.0%
! AGTGAGAGCCTTATATACTT+TGG + chr3.2:92968635-92968654 MS.gene68750:intron 35.0%
! ATAACTCAGAAAGCGTGAAT+GGG - chr3.2:92968761-92968780 None:intergenic 35.0%
! CATGGTTCGATAAGAAGTAT+AGG - chr3.2:92968470-92968489 None:intergenic 35.0%
! CATGTATCGTGAGTTGAAAA+GGG - chr3.2:92968183-92968202 None:intergenic 35.0%
! CCATGAATCATTCATTGGTT+AGG + chr3.2:92968692-92968711 MS.gene68750:intron 35.0%
! CTATTTGGTTTCATCAAGCT+AGG - chr3.2:92969231-92969250 None:intergenic 35.0%
! GTGAGAGCCTTATATACTTT+GGG + chr3.2:92968636-92968655 MS.gene68750:intron 35.0%
! TACTACTGTTGTTGTTGTTG+AGG + chr3.2:92968897-92968916 MS.gene68750:CDS 35.0%
! TGTTGTTGTTGTTGAGGAAA+TGG + chr3.2:92968903-92968922 MS.gene68750:CDS 35.0%
! TTTGGTTCATACTCCTTCAA+CGG - chr3.2:92969512-92969531 None:intergenic 35.0%
!! AGGTTGATTTGAAAGACAAG+TGG + chr3.2:92970089-92970108 MS.gene68750:intron 35.0%
!! CATCTTCCGATTATCGATTT+CGG - chr3.2:92967811-92967830 None:intergenic 35.0%
!!! AACTTTTTTTGGAGGATGCT+CGG + chr3.2:92968255-92968274 MS.gene68750:CDS 35.0%
!!! AGAGGTTCAACTTTTTTTGG+AGG + chr3.2:92968247-92968266 MS.gene68750:CDS 35.0%
!!! TTACTCTGGTTTTCCATTTG+TGG - chr3.2:92969111-92969130 None:intergenic 35.0%
AAAATGGAGTCAATGGGAAG+AGG + chr3.2:92969559-92969578 MS.gene68750:CDS 40.0%
AACTCGACAAAGACAATGGA+AGG + chr3.2:92968857-92968876 MS.gene68750:CDS 40.0%
ACATCAGACCTTACAATGTC+TGG - chr3.2:92969063-92969082 None:intergenic 40.0%
AGACATTGTAAGGTCTGATG+TGG + chr3.2:92969062-92969081 MS.gene68750:CDS 40.0%
ATATTCTGTCAACTGCTTGG+AGG - chr3.2:92968076-92968095 None:intergenic 40.0%
ATGGAACTCGACAAAGACAA+TGG + chr3.2:92968853-92968872 MS.gene68750:CDS 40.0%
CAACAGAGTGAAGAAAGGAA+GGG - chr3.2:92968295-92968314 None:intergenic 40.0%
CAGTCACCGAAATCGATAAT+CGG + chr3.2:92967802-92967821 MS.gene68750:CDS 40.0%
CCATTCATCGATAGATGCTT+TGG - chr3.2:92967874-92967893 None:intergenic 40.0%
CTTCATTGTCTGATACCAGA+AGG + chr3.2:92968213-92968232 MS.gene68750:CDS 40.0%
GAAGAGGATAATCTAAGGAC+CGG + chr3.2:92969575-92969594 MS.gene68750:CDS 40.0%
GATTTCATGGCATCGCTAAT+AGG - chr3.2:92967969-92967988 None:intergenic 40.0%
GGCAATTGAAGACACAAATC+AGG + chr3.2:92969083-92969102 MS.gene68750:CDS 40.0%
GGTAAAAGCAAAAGGAGATG+AGG - chr3.2:92969696-92969715 None:intergenic 40.0%
GGTCTCCATGAATCATTCAT+TGG + chr3.2:92968687-92968706 MS.gene68750:intron 40.0%
GTGTCAAGTCAACATTGTGT+CGG - chr3.2:92968500-92968519 None:intergenic 40.0%
TCAGAATTGGAGAAGGAAAC+TGG + chr3.2:92969951-92969970 MS.gene68750:intron 40.0%
TCCAAGCAGTTGACAGAATA+TGG + chr3.2:92968075-92968094 MS.gene68750:CDS 40.0%
TCCATATTCTGTCAACTGCT+TGG - chr3.2:92968079-92968098 None:intergenic 40.0%
TCTCATATTAGAGTCTCCGA+TGG + chr3.2:92968834-92968853 MS.gene68750:intron 40.0%
TGCTTATGTCTACATTGCTG+TGG + chr3.2:92967989-92968008 MS.gene68750:CDS 40.0%
TGGACAACAACTCATCTTCA+AGG - chr3.2:92967854-92967873 None:intergenic 40.0%
TTGACAGAATATGGAACCGT+AGG + chr3.2:92968084-92968103 MS.gene68750:CDS 40.0%
TTGTCTTTGTCGAGTTCCAT+CGG - chr3.2:92968853-92968872 None:intergenic 40.0%
! AAGTGAATCCCCGTTCTTTT+GGG - chr3.2:92968977-92968996 None:intergenic 40.0%
! AGAGTCAATTTTGCTACAGC+TGG - chr3.2:92968938-92968957 None:intergenic 40.0%
! CATAACTCAGAAAGCGTGAA+TGG - chr3.2:92968762-92968781 None:intergenic 40.0%
! CCAAAGCATCTATCGATGAA+TGG + chr3.2:92967871-92967890 MS.gene68750:CDS 40.0%
! GCATGTATCGTGAGTTGAAA+AGG - chr3.2:92968184-92968203 None:intergenic 40.0%
! TAACTCAGAAAGCGTGAATG+GGG - chr3.2:92968760-92968779 None:intergenic 40.0%
! TAAGTGAATCCCCGTTCTTT+TGG - chr3.2:92968978-92968997 None:intergenic 40.0%
! TGGTGTTTGGAATTCGTTCT+TGG + chr3.2:92967720-92967739 MS.gene68750:CDS 40.0%
!! ATTATCGATTTCGGTGACTG+AGG - chr3.2:92967802-92967821 None:intergenic 40.0%
!! CATCGCTAATAGGTGGATTA+GGG - chr3.2:92967959-92967978 None:intergenic 40.0%
!! GATTGATTTTGAGCAGCAGA+AGG - chr3.2:92968323-92968342 None:intergenic 40.0%
!! GTGGATTAGGGTTTGAAGAA+GGG - chr3.2:92967947-92967966 None:intergenic 40.0%
!! TGAAAAGGGAAGCATCGATT+CGG - chr3.2:92968169-92968188 None:intergenic 40.0%
ACTGCTTGGAGGTAGTGTTT+AGG - chr3.2:92968065-92968084 None:intergenic 45.0%
AGCAGCAACAGAGTGAAGAA+AGG - chr3.2:92968300-92968319 None:intergenic 45.0%
ATGGATGCTTGAACTTCTCG+AGG + chr3.2:92967890-92967909 MS.gene68750:CDS 45.0%
ATTGCAGGCAGGAACATCAA+GGG + chr3.2:92969439-92969458 MS.gene68750:CDS 45.0%
ATTTAGGCAAGCATGTGGGT+TGG - chr3.2:92969148-92969167 None:intergenic 45.0%
CAATGGAAGGCTTAAAGGCA+AGG + chr3.2:92968870-92968889 MS.gene68750:CDS 45.0%
CACAAATCAGGAGCCACAAA+TGG + chr3.2:92969095-92969114 MS.gene68750:CDS 45.0%
CAGAAGGAAAGCCATTCAAG+AGG + chr3.2:92968229-92968248 MS.gene68750:CDS 45.0%
CGTAGGTTTCAAAACCTGGA+GGG + chr3.2:92968101-92968120 MS.gene68750:CDS 45.0%
CTCTTGAATGGCTTTCCTTC+TGG - chr3.2:92968231-92968250 None:intergenic 45.0%
CTGCTTGGAGGTAGTGTTTA+GGG - chr3.2:92968064-92968083 None:intergenic 45.0%
GAACCGTAGGTTTCAAAACC+TGG + chr3.2:92968097-92968116 MS.gene68750:CDS 45.0%
GAAGAAGCTTTCTCCGTTGA+AGG + chr3.2:92969496-92969515 MS.gene68750:CDS 45.0%
GATGCTCGGAATAACCTTGT+AGG + chr3.2:92968269-92968288 MS.gene68750:CDS 45.0%
GCAAATAACTTCTTCCCTCC+AGG - chr3.2:92968118-92968137 None:intergenic 45.0%
GCAACAGAGTGAAGAAAGGA+AGG - chr3.2:92968296-92968315 None:intergenic 45.0%
GGAAGTTGCCTTCGTAAGAT+TGG - chr3.2:92969187-92969206 None:intergenic 45.0%
GTTAACGAGGCCCAAAAGAA+CGG + chr3.2:92968964-92968983 MS.gene68750:CDS 45.0%
TAGTGGGATGATTCGAACGA+TGG + chr3.2:92969416-92969435 MS.gene68750:intron 45.0%
TGACTCTTTGCCAGTTAACG+AGG + chr3.2:92968951-92968970 MS.gene68750:CDS 45.0%
TGATGAGGCCAATCTTACGA+AGG + chr3.2:92969176-92969195 MS.gene68750:CDS 45.0%
TTAACGAGGCCCAAAAGAAC+GGG + chr3.2:92968965-92968984 MS.gene68750:CDS 45.0%
TTCATGGCATCGCTAATAGG+TGG - chr3.2:92967966-92967985 None:intergenic 45.0%
TTGCTGTGGAATGTACCGTT+AGG + chr3.2:92968003-92968022 MS.gene68750:CDS 45.0%
TTTAGGCAAGCATGTGGGTT+GGG - chr3.2:92969147-92969166 None:intergenic 45.0%
! AACTCAGAAAGCGTGAATGG+GGG - chr3.2:92968759-92968778 None:intergenic 45.0%
! GCATCGCTAATAGGTGGATT+AGG - chr3.2:92967960-92967979 None:intergenic 45.0%
! TAGTTCTGCTCTTACTCACG+AGG + chr3.2:92969248-92969267 MS.gene68750:CDS 45.0%
! TGCTGCTTCAAGGTACCTAA+CGG - chr3.2:92968021-92968040 None:intergenic 45.0%
! TGGAGAAATTTGAAGCGGTC+TGG + chr3.2:92970109-92970128 MS.gene68750:CDS 45.0%
! TTCTTTTGGGCCTCGTTAAC+TGG - chr3.2:92968964-92968983 None:intergenic 45.0%
!! GGTGGATTAGGGTTTGAAGA+AGG - chr3.2:92967948-92967967 None:intergenic 45.0%
!! TGATTCGAACGATGGATTGC+AGG + chr3.2:92969424-92969443 MS.gene68750:CDS 45.0%
!!! CCTCCAGGTTTTGAAACCTA+CGG - chr3.2:92968103-92968122 None:intergenic 45.0%
AAAGCGTGAATGGGGGATTC+AGG - chr3.2:92968752-92968771 None:intergenic 50.0%
AAGCGTGAATGGGGGATTCA+GGG - chr3.2:92968751-92968770 None:intergenic 50.0%
CCGTAGGTTTCAAAACCTGG+AGG + chr3.2:92968100-92968119 MS.gene68750:CDS 50.0%
GAAGAAAGGAAGGGCCTACA+AGG - chr3.2:92968286-92968305 None:intergenic 50.0%
GATTGCAGGCAGGAACATCA+AGG + chr3.2:92969438-92969457 MS.gene68750:CDS 50.0%
GGAAAGGTTAGGGTGGTGTT+TGG + chr3.2:92967707-92967726 MS.gene68750:CDS 50.0%
GGAGAAAGCTTCTTCCTCTC+TGG - chr3.2:92969491-92969510 None:intergenic 50.0%
GTTAGGTACCTTGAAGCAGC+AGG + chr3.2:92968020-92968039 MS.gene68750:CDS 50.0%
TAACGAGGCCCAAAAGAACG+GGG + chr3.2:92968966-92968985 MS.gene68750:CDS 50.0%
TTCGTTTGCGTAGTCCAGAG+AGG + chr3.2:92969474-92969493 MS.gene68750:CDS 50.0%
TTGCAGGCAGGAACATCAAG+GGG + chr3.2:92969440-92969459 MS.gene68750:CDS 50.0%
!! CATTGTGTCGGTGTCAGACA+TGG - chr3.2:92968488-92968507 None:intergenic 50.0%
TACCTTGAAGCAGCAGGTGC+AGG + chr3.2:92968026-92968045 MS.gene68750:CDS 55.0%
!! TCGAACGATGGATTGCAGGC+AGG + chr3.2:92969428-92969447 MS.gene68750:CDS 55.0%
CGTGAATGGGGGATTCAGGG+AGG - chr3.2:92968748-92968767 None:intergenic 60.0%
CTTGAAGCAGCAGGTGCAGG+TGG + chr3.2:92968029-92968048 MS.gene68750:CDS 60.0%
! CACCTGCACCTGCTGCTTCA+AGG - chr3.2:92968031-92968050 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 92967705 92970141 92967705 ID=MS.gene68750
chr3.2 mRNA 92967705 92970141 92967705 ID=MS.gene68750.t1;Parent=MS.gene68750
chr3.2 exon 92967705 92968350 92967705 ID=MS.gene68750.t1.exon1;Parent=MS.gene68750.t1
chr3.2 CDS 92967705 92968350 92967705 ID=cds.MS.gene68750.t1;Parent=MS.gene68750.t1
chr3.2 exon 92968845 92969269 92968845 ID=MS.gene68750.t1.exon2;Parent=MS.gene68750.t1
chr3.2 CDS 92968845 92969269 92968845 ID=cds.MS.gene68750.t1;Parent=MS.gene68750.t1
chr3.2 exon 92969419 92969606 92969419 ID=MS.gene68750.t1.exon3;Parent=MS.gene68750.t1
chr3.2 CDS 92969419 92969606 92969419 ID=cds.MS.gene68750.t1;Parent=MS.gene68750.t1
chr3.2 exon 92969955 92970015 92969955 ID=MS.gene68750.t1.exon4;Parent=MS.gene68750.t1
chr3.2 CDS 92969955 92970015 92969955 ID=cds.MS.gene68750.t1;Parent=MS.gene68750.t1
chr3.2 exon 92970091 92970141 92970091 ID=MS.gene68750.t1.exon5;Parent=MS.gene68750.t1
chr3.2 CDS 92970091 92970141 92970091 ID=cds.MS.gene68750.t1;Parent=MS.gene68750.t1
Gene Sequence

>MS.gene68750

ATGGAAAGGTTAGGGTGGTGTTTGGAATTCGTTCTTGGACATTCTTCAACAAGTATAGAGAGTGAGCATATTAAGGAAATCTTGAAACTAAGCTCCTCAGTCACCGAAATCGATAATCGGAAGATGAAAATGCTTCTGTTGGAAGGCCTTGAAGATGAGTTGTTGTCCAAAGCATCTATCGATGAATGGATGCTTGAACTTCTCGAGGTATTCGAAAAACTACTTCTAATTGATTGGGACCCTTCTTCAAACCCTAATCCACCTATTAGCGATGCCATGAAATCTGCTTATGTCTACATTGCTGTGGAATGTACCGTTAGGTACCTTGAAGCAGCAGGTGCAGGTGGTATATCTAACCCTAAACACTACCTCCAAGCAGTTGACAGAATATGGAACCGTAGGTTTCAAAACCTGGAGGGAAGAAGTTATTTGCTAACTCCAAAATTGAGATTCTGGAAAAGCCGAATCGATGCTTCCCTTTTCAACTCACGATACATGCTCAAACTTGCTTCATTGTCTGATACCAGAAGGAAAGCCATTCAAGAGGTTCAACTTTTTTTGGAGGATGCTCGGAATAACCTTGTAGGCCCTTCCTTTCTTCACTCTGTTGCTGCTCCTTCTGCTGCTCAAAATCAATCCCATAATGGTTGGTTATTTCTTTTCCCCTTTTTTCTGTTGTTGTTGTTGTTGTTTATTACCGCTTTATATATATCTCTCTCTCTCTCTCATACACTAGTTACTTACTTGTCTTTGAAGCAAGCACCTATACTTCTTATCGAACCATGTCTGACACCGACACAATGTTGACTTGACACATGTAATTACAATCAATTATGTCATTTTCTCAAATTATTATCCGTATATGTGCTTGTGCTTCATAGGTAGTAGTTAATTGACTTTTTAAGAAGAGTAAATAATTGCAATTAAGTGAGTGAGAGCCTTATATACTTTGGGAGAGAGAATTTCATTGCAAAATGCATAGGGTCTCCATGAATCATTCATTGGTTAGGACTGACAAATCTAAATCATTGATTTTCATTCCTCCCTGAATCCCCCATTCACGCTTTCTGAGTTATGAAAAATACCTGTTTGCTTTTTAGAACTTTTAATCACATTTTGTTTTTCTTCTTCTCATATTAGAGTCTCCGATGGAACTCGACAAAGACAATGGAAGGCTTAAAGGCAAGGCTTCTACTACTGTTGTTGTTGTTGAGGAAATGGCTACATCAACCAGCTGTAGCAAAATTGACTCTTTGCCAGTTAACGAGGCCCAAAAGAACGGGGATTCACTTAAATGCAATTCTGTGGAGTTCAAAACATTTGCGAACGATCCTGTGCCCAATTCTATTTGTATGCCAGACATTGTAAGGTCTGATGTGGCAATTGAAGACACAAATCAGGAGCCACAAATGGAAAACCAGAGTAAAGATGCAAATGTACCCAACCCACATGCTTGCCTAAATGTTAACAATGATGAGGCCAATCTTACGAAGGCAACTTCCCATCAAAGTGATGTTCATCATCCTAGCTTGATGAAACCAAATAGTTCTGCTCTTACTCACGAGGTAAATGTGTTTTGTAATTTTTTGTATTTCTGGTTGATTCCTTTTTACTCTAAAAACACTAGTTTGTTGAAACAAAATATCTCATGAACAACCATGCTTGTAATTTTTTGTTCTTCCGATGCCTTGATTTTATTGCTTATTTTATGTAGTGGGATGATTCGAACGATGGATTGCAGGCAGGAACATCAAGGGGCACAAGTAGAATTCGTTTGCGTAGTCCAGAGAGGAAGAAGCTTTCTCCGTTGAAGGAGTATGAACCAAAAAATATTACGAAAAGGAGAAAGAAAAAAAAATGGAGTCAATGGGAAGAGGATAATCTAAGGACCGGAATTAAACTGTATGTTCTTGTTTTAACCAATCAAGTTACTATAATATTTTGTTCTGTATTTCATTGATACAATGTTTTTTCGTTTTTGGTCAGTTCCTCATCTCCTTTTGCTTTTACCTTTACTTTGTCATACATTTTTTAAATATTTGATAGTGTGTGAAATTCTGATATTTTTAGTGTCTATGCATAAGAGAATATGTTTCATCTCTCAACAGTCACATATCTCACCATTTTACTATTCAAATTATTTTACCCTAACTATTTATGCTTCAATTGAGAATATAGATAGAATTAAATTCACTTTCACTACAAATTCATATTCTCATTTATCTTTCTTTTTCTTTTTTCTTTTTTCAGAATTGGAGAAGGAAACTGGAAATCAATTCTTCGTTCTTATGAATTTGACGAAAGAACTGAAGTAAGTCTAGTAAAGGTATTGGTATTTTCTTTATTCTGCTTATGCTTCTAACGTGAATCAAATTTTGTTTATCAGGTTGATTTGAAAGACAAGTGGAGAAATTTGAAGCGGTCTGGATGCCAATGA
Protein sequence

>MS.gene68750.t1

MERLGWCLEFVLGHSSTSIESEHIKEILKLSSSVTEIDNRKMKMLLLEGLEDELLSKASIDEWMLELLEVFEKLLLIDWDPSSNPNPPISDAMKSAYVYIAVECTVRYLEAAGAGGISNPKHYLQAVDRIWNRRFQNLEGRSYLLTPKLRFWKSRIDASLFNSRYMLKLASLSDTRRKAIQEVQLFLEDARNNLVGPSFLHSVAAPSAAQNQSHNESPMELDKDNGRLKGKASTTVVVVEEMATSTSCSKIDSLPVNEAQKNGDSLKCNSVEFKTFANDPVPNSICMPDIVRSDVAIEDTNQEPQMENQSKDANVPNPHACLNVNNDEANLTKATSHQSDVHHPSLMKPNSSALTHEWDDSNDGLQAGTSRGTSRIRLRSPERKKLSPLKEYEPKNITKRRKKKKWSQWEEDNLRTGIKLIGEGNWKSILRSYEFDERTEVDLKDKWRNLKRSGCQ