AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68828.t1 MTR_6g051425 33.333 273 173 4 4 267 19 291 7.43e-40 150
MS.gene68828.t1 MTR_7g446060 72.527 91 25 0 79 169 2 92 8.00e-40 143
MS.gene68828.t1 MTR_2g090105 47.283 184 81 6 580 761 16 185 1.44e-37 139
MS.gene68828.t1 MTR_7g031620 79.268 82 17 0 7 88 1 82 6.96e-35 127
MS.gene68828.t1 MTR_5g023310 41.401 157 25 2 133 289 16 105 9.86e-30 114
MS.gene68828.t1 MTR_0558s0020 29.457 258 154 7 7 238 41 296 1.67e-25 107
MS.gene68828.t1 MTR_4g035820 32.422 256 154 9 1 242 74 324 5.95e-25 111
MS.gene68828.t1 MTR_8g446020 29.834 181 121 3 62 238 78 256 2.88e-20 91.7
MS.gene68828.t1 MTR_0856s0010 36.220 127 80 1 2 127 34 160 6.90e-19 84.7
MS.gene68828.t1 MTR_6g090505 29.444 180 121 3 62 237 53 230 6.29e-17 83.6
MS.gene68828.t1 MTR_1g083640 27.074 229 139 7 7 209 56 282 1.14e-16 81.6
MS.gene68828.t1 MTR_1g008290 35.659 129 80 2 66 194 19 144 6.42e-16 75.9
MS.gene68828.t1 MTR_1g070355 28.692 237 150 9 8 232 80 309 2.07e-15 80.5
MS.gene68828.t1 MTR_6g022070 28.767 146 100 1 292 437 1 142 2.28e-15 78.6
MS.gene68828.t1 MTR_1g013060 29.665 209 132 6 2 198 24 229 2.18e-14 73.9
MS.gene68828.t1 MTR_7g446480 33.735 166 105 4 84 246 151 314 3.75e-14 74.7
MS.gene68828.t1 MTR_7g446510 33.133 166 106 4 84 246 166 329 7.81e-14 73.9
MS.gene68828.t1 MTR_8g465780 31.579 114 72 2 368 480 1 109 8.91e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68828.t1 AT4G15090 31.319 728 470 12 2 722 51 755 7.32e-123 388
MS.gene68828.t1 AT4G15090 31.319 728 470 12 2 722 51 755 7.32e-123 388
MS.gene68828.t1 AT4G15090 31.319 728 470 12 2 722 51 755 7.32e-123 388
MS.gene68828.t1 AT4G15090 31.319 728 470 12 2 722 51 755 7.32e-123 388
MS.gene68828.t1 AT4G15090 31.319 728 470 12 2 722 51 755 7.32e-123 388
MS.gene68828.t1 AT4G38180 35.951 573 343 10 2 561 73 634 1.57e-116 370
MS.gene68828.t1 AT1G76320 35.552 571 332 9 8 566 3 549 9.95e-113 357
MS.gene68828.t1 AT1G76320 35.552 571 332 9 8 566 3 549 2.73e-112 358
MS.gene68828.t1 AT1G76320 35.552 571 332 9 8 566 3 549 3.12e-112 357
MS.gene68828.t1 AT1G76320 35.552 571 332 9 8 566 3 549 3.12e-112 357
MS.gene68828.t1 AT1G52520 34.790 572 349 13 2 564 85 641 1.23e-110 352
MS.gene68828.t1 AT3G22170 34.423 581 334 9 2 564 70 621 3.42e-110 355
MS.gene68828.t1 AT3G22170 34.423 581 334 9 2 564 70 621 3.42e-110 355
MS.gene68828.t1 AT1G80010 33.275 574 364 11 2 562 95 662 1.08e-99 324
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 24 564 9.54e-94 309
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 24 564 9.54e-94 309
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 24 564 1.31e-93 309
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 38 578 1.50e-93 309
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 24 564 1.95e-93 309
MS.gene68828.t1 AT4G19990 33.505 582 326 13 5 566 24 564 1.95e-93 309
MS.gene68828.t1 AT2G27110 32.337 569 338 12 2 561 49 579 9.59e-89 298
MS.gene68828.t1 AT2G27110 32.337 569 338 12 2 561 49 579 9.59e-89 298
MS.gene68828.t1 AT4G19990 34.462 502 285 9 68 566 47 507 1.55e-88 294
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 135 561 1.16e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 135 561 1.20e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 1.21e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 2.11e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 2.11e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 2.80e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 2.80e-85 288
MS.gene68828.t1 AT2G32250 34.921 441 268 7 178 613 176 602 2.80e-85 288
MS.gene68828.t1 AT2G27110 35.374 441 260 9 126 561 14 434 2.44e-83 280
MS.gene68828.t1 AT4G38170 34.466 412 252 8 167 573 13 411 1.31e-77 260
MS.gene68828.t1 AT3G06250 30.249 562 339 11 2 559 190 702 1.52e-75 260
MS.gene68828.t1 AT3G06250 30.249 562 339 11 2 559 190 702 1.52e-75 260
MS.gene68828.t1 AT3G06250 30.249 562 339 11 2 559 190 702 1.52e-75 260
MS.gene68828.t1 AT5G18960 30.864 567 332 14 2 559 211 726 3.39e-75 260
MS.gene68828.t1 AT5G18960 30.864 567 332 14 2 559 211 726 3.39e-75 260
MS.gene68828.t1 AT1G10240 27.973 597 352 21 1 558 47 604 1.40e-64 228
MS.gene68828.t1 AT1G10240 27.973 597 352 21 1 558 47 604 1.40e-64 228
MS.gene68828.t1 AT5G28530 26.667 585 378 17 2 559 55 615 1.07e-49 186
MS.gene68828.t1 AT5G28530 26.667 585 378 17 2 559 55 615 1.07e-49 186
MS.gene68828.t1 AT5G28530 26.655 589 377 18 2 559 55 619 7.72e-49 184
MS.gene68828.t1 AT5G28530 24.433 573 343 19 2 559 55 552 1.45e-32 134
MS.gene68828.t1 AT5G28530 24.437 577 342 20 2 559 55 556 9.43e-32 132

Find 106 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTGGTGAATGCTTTC+TGG 0.174694 6.1:+80152227 None:intergenic
CCATCTTCTTCTATAATTTC+AGG 0.175398 6.1:+80151713 None:intergenic
CATATGTGAGAGACACATTT+TGG 0.184902 6.1:-80152453 MS.gene68828:CDS
TATCAAAACCACATTTAATA+AGG 0.185554 6.1:+80151891 None:intergenic
TCACTCATAAGTATTGAATT+TGG 0.202186 6.1:+80151221 None:intergenic
CAACCTCCACTTGAAATTCT+TGG 0.215469 6.1:+80152200 None:intergenic
GTCGTTTAAGAAATGTATTT+TGG 0.215986 6.1:-80152990 MS.gene68828:CDS
AAGGAAAACATGGCTGATTT+TGG 0.228183 6.1:-80152304 MS.gene68828:CDS
CACCATCGATGGCGAGCTTT+TGG 0.238537 6.1:+80152685 None:intergenic
TTTGAGTAAAAGTGATTTCA+TGG 0.249102 6.1:-80152566 MS.gene68828:CDS
ATAGCTTGTGTAAGTCATTT+TGG 0.262515 6.1:-80152265 MS.gene68828:CDS
CGTAGAAGCAACTTCGTAAA+AGG 0.287052 6.1:+80151820 None:intergenic
TTCATGACTTGAGGATAAAA+TGG 0.287642 6.1:+80151103 None:intergenic
CTTTGTGTGCTTAAGCTAAC+AGG 0.298887 6.1:-80151983 MS.gene68828:CDS
TATGCCCGGTCTATAGGTTT+TGG 0.300318 6.1:-80153525 MS.gene68828:CDS
TGATCAGTTATTATGGCATT+TGG 0.323265 6.1:+80152745 None:intergenic
TGGTGAATGCTTTCTGGAAT+TGG 0.326923 6.1:+80152233 None:intergenic
CTCAAAGTCTTTCTCATTGA+AGG 0.328946 6.1:+80152060 None:intergenic
AGGGCTGCATATGAATATTT+TGG 0.330794 6.1:-80152952 MS.gene68828:CDS
AAGTGCGGAGACTGCGACTT+GGG 0.331491 6.1:-80153104 MS.gene68828:CDS
CGCAGTGGAGGAAGGATATA+AGG 0.337986 6.1:-80151925 MS.gene68828:CDS
TTTGTGGCATATAATGAAAA+AGG 0.344495 6.1:-80152662 MS.gene68828:CDS
AGTATGAACTCATTCTTTGA+TGG 0.348820 6.1:-80152400 MS.gene68828:CDS
ATTGTCTTGATCAGTTATTA+TGG 0.355900 6.1:+80152738 None:intergenic
AAGAGTTATGCCCGGTCTAT+AGG 0.361091 6.1:-80153531 MS.gene68828:CDS
ACTTATGAGTGAAGTATATC+AGG 0.377433 6.1:-80151210 MS.gene68828:intron
TCATGCTTGCTTTGATAGAT+TGG 0.388712 6.1:-80152161 MS.gene68828:CDS
ATCAAAACCACATTTAATAA+GGG 0.391774 6.1:+80151892 None:intergenic
CATCTTCTTCTATAATTTCA+GGG 0.405722 6.1:+80151714 None:intergenic
CCTTTACTTCGAGTCGGTAC+AGG 0.409568 6.1:+80151656 None:intergenic
AATTAAAGACATAGTGTTTG+AGG 0.424296 6.1:-80152086 MS.gene68828:CDS
AAAGTGATTTCATGGAAAAG+TGG 0.450883 6.1:-80152558 MS.gene68828:CDS
AAGTATTGAATTTGGATTGA+AGG 0.452798 6.1:+80151229 None:intergenic
CTCGCCATCGATGGTGTTTG+TGG 0.452930 6.1:-80152678 MS.gene68828:CDS
ATTTGATGAAAGAAATCGTT+GGG 0.454237 6.1:-80152482 MS.gene68828:CDS
GAATATGACATTTGGTGAAA+AGG 0.456326 6.1:-80153148 MS.gene68828:CDS
CCATGGTCAATCTGTGTTGT+TGG 0.460551 6.1:-80152851 MS.gene68828:CDS
CATGGTCAATCTGTGTTGTT+GGG 0.466450 6.1:-80152850 MS.gene68828:CDS
ACTCCAACAAAAGGAGCAAA+TGG 0.472118 6.1:+80152880 None:intergenic
TGTTGTTTGTTTCAATTCAA+AGG 0.475247 6.1:-80152025 MS.gene68828:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.483477 6.1:-80152972 MS.gene68828:CDS
AAGGTCGTCCTCCTTCAAAA+AGG 0.483624 6.1:-80151637 MS.gene68828:CDS
AGTATTGAATTTGGATTGAA+GGG 0.484124 6.1:+80151230 None:intergenic
TTGGCTGTTCACAACATGGT+TGG 0.487761 6.1:-80152788 MS.gene68828:CDS
CCTGAAATTATAGAAGAAGA+TGG 0.488011 6.1:-80151713 MS.gene68828:CDS
CTAAATATAGTTCCAACCTT+AGG 0.488331 6.1:+80153582 None:intergenic
TATCCTCAAGTCATGAATCA+TGG 0.488967 6.1:-80151097 MS.gene68828:CDS
CGGAGACTGCGACTTGGGGT+TGG 0.494185 6.1:-80153099 MS.gene68828:CDS
GTTCAAGTAGTTGATAAAGT+TGG 0.495312 6.1:-80151418 MS.gene68828:CDS
CTATAGTTGTTGAAGCGAAT+GGG 0.503159 6.1:-80153176 MS.gene68828:CDS
AAGTGATTTCATGGAAAAGT+GGG 0.506412 6.1:-80152557 MS.gene68828:CDS
TCTATAGTTGTTGAAGCGAA+TGG 0.507984 6.1:-80153177 MS.gene68828:CDS
CCACTGCGAGAAAATATAAG+AGG 0.509446 6.1:+80151940 None:intergenic
AATGTGTCTCTCACATATGC+AGG 0.509862 6.1:+80152457 None:intergenic
CATTCAATCTAGCCTTGCAC+TGG 0.510411 6.1:+80153376 None:intergenic
AAACACTATGTCTTTAATTG+TGG 0.512674 6.1:+80152090 None:intergenic
AATGCGTGTATTTCTTTAGA+CGG 0.517437 6.1:-80153357 MS.gene68828:CDS
GCTTGCTTTGATAGATTGGA+AGG 0.517556 6.1:-80152157 MS.gene68828:CDS
ATTGCTCTTGAGCATAATCA+TGG 0.525914 6.1:-80153315 MS.gene68828:CDS
TGGTGATTTACTCCAACAAA+AGG 0.525957 6.1:+80152871 None:intergenic
TCAGGATAACGAATGGTTGA+AGG 0.526171 6.1:-80152509 MS.gene68828:CDS
TGGATCCTCGTTTGAAAAGG+AGG 0.528466 6.1:-80153248 MS.gene68828:CDS
GGGTATGAGAATATGACATT+TGG 0.528947 6.1:-80153156 MS.gene68828:CDS
AAAGATGAAATGCCTAAGGT+TGG 0.530444 6.1:-80153594 None:intergenic
TTCCAAAAGCTCGCCATCGA+TGG 0.531681 6.1:-80152687 MS.gene68828:CDS
TTGGGCAGATGCGAGATGTA+GGG 0.538615 6.1:-80152971 MS.gene68828:CDS
TGTTGTGAACAGCCAAGTAA+AGG 0.547394 6.1:+80152795 None:intergenic
TAACTCCAAAACCTATAGAC+CGG 0.548272 6.1:+80153520 None:intergenic
AGGATACTAAGACCTTTACT+TGG 0.548532 6.1:-80152807 MS.gene68828:CDS
CAGGATAACGAATGGTTGAA+GGG 0.548682 6.1:-80152508 MS.gene68828:CDS
CCTGTACCGACTCGAAGTAA+AGG 0.550769 6.1:-80151656 MS.gene68828:CDS
CTTTACTTCGAGTCGGTACA+GGG 0.551509 6.1:+80151657 None:intergenic
AGGCATACCCTTATTAAATG+TGG 0.553411 6.1:-80151899 MS.gene68828:CDS
TTACTTGGCTGTTCACAACA+TGG 0.555090 6.1:-80152792 MS.gene68828:CDS
ATTCCAAGAATTTCAAGTGG+AGG 0.555252 6.1:-80152203 MS.gene68828:CDS
TTCAGGGCATGTTTCTTGAG+AGG 0.556231 6.1:+80151730 None:intergenic
ATTGAAAGATAAAGTTGAGA+AGG 0.561801 6.1:-80152323 MS.gene68828:CDS
TTAGAACTCAATGATCAAGC+TGG 0.567016 6.1:-80153225 MS.gene68828:CDS
AAAGTGCGGAGACTGCGACT+TGG 0.575984 6.1:-80153105 MS.gene68828:CDS
TCGCTTCAACAACTATAGAT+CGG 0.588019 6.1:+80153181 None:intergenic
ACATGGTTGGAATGCATGCA+TGG 0.592737 6.1:-80152775 MS.gene68828:CDS
TTGAACTTCAGGATAACGAA+TGG 0.592982 6.1:-80152516 MS.gene68828:CDS
TCATAAAGATGAAATGCCTA+AGG 0.597014 6.1:-80153598 None:intergenic
ACACCATGATTCATGACTTG+AGG 0.597741 6.1:+80151094 None:intergenic
CATTTGGTGAAAAGGACTGT+AGG 0.603039 6.1:-80153140 MS.gene68828:CDS
TATTTGATGAAAGAAATCGT+TGG 0.605775 6.1:-80152483 MS.gene68828:CDS
AACTCCAAAACCTATAGACC+GGG 0.618933 6.1:+80153521 None:intergenic
CTGTTAGCTTAAGCACACAA+AGG 0.625605 6.1:+80151984 None:intergenic
TGCGAGAAAATATAAGAGGA+CGG 0.628214 6.1:+80151944 None:intergenic
AGTATTACAAGAGTTATGCC+CGG 0.630025 6.1:-80153539 MS.gene68828:CDS
AGTGCGGAGACTGCGACTTG+GGG 0.633117 6.1:-80153103 MS.gene68828:CDS
TAAAGTTGAGAAGGAAAACA+TGG 0.634633 6.1:-80152314 MS.gene68828:CDS
TATGCCACAAACACCATCGA+TGG 0.638359 6.1:+80152674 None:intergenic
TTTACTCTAGCATGTAGTCG+TGG 0.642195 6.1:-80153456 MS.gene68828:CDS
AAAATTCCAAGAATTTCAAG+TGG 0.644675 6.1:-80152206 MS.gene68828:CDS
ACTTGGGGTTGGAGATGCTG+AGG 0.644897 6.1:-80153088 MS.gene68828:CDS
GGAGGAAGGATATAAGGCGA+AGG 0.647816 6.1:-80151919 MS.gene68828:CDS
AGGATAACGAATGGTTGAAG+GGG 0.650586 6.1:-80152507 MS.gene68828:CDS
CTTGCAATTTAGATGCACAC+AGG 0.652836 6.1:+80151446 None:intergenic
TTTGTTGGAGTAAATCACCA+TGG 0.661689 6.1:-80152868 MS.gene68828:CDS
TTTAGGAGGAATGAACATGG+TGG 0.670141 6.1:-80151269 MS.gene68828:CDS
CGTGTTGTTGATGATGACGA+TGG 0.670761 6.1:+80150940 None:intergenic
ATGTGCTTTACTGTCAAGCG+AGG 0.675011 6.1:-80152827 MS.gene68828:CDS
CCAACAACACAGATTGACCA+TGG 0.675112 6.1:+80152851 None:intergenic
GGACGACCTTTACTTCGAGT+CGG 0.678639 6.1:+80151650 None:intergenic
GGAACTATATAGATAAAGTG+CGG 0.685509 6.1:-80153119 MS.gene68828:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAAAAGAAACATAAG+AGG + chr6.1:80153487-80153506 None:intergenic 15.0%
!!! TTATTACATGTATGTTTTTA+GGG - chr6.1:80153210-80153229 MS.gene68828:CDS 15.0%
!!! TTCATATTTTGAATTATTAC+AGG - chr6.1:80153444-80153463 MS.gene68828:CDS 15.0%
!!! TTTATTACATGTATGTTTTT+AGG - chr6.1:80153209-80153228 MS.gene68828:CDS 15.0%
!!! TTTTAGAAGTAATAAGATTT+TGG - chr6.1:80151247-80151266 MS.gene68828:CDS 15.0%
!! ATCAAAACCACATTTAATAA+GGG + chr6.1:80152626-80152645 None:intergenic 20.0%
!! TATCAAAACCACATTTAATA+AGG + chr6.1:80152627-80152646 None:intergenic 20.0%
!!! GTCTAATTTTCAATATTTGA+TGG - chr6.1:80153041-80153060 MS.gene68828:CDS 20.0%
!!! TATTACATGTATGTTTTTAG+GGG - chr6.1:80153211-80153230 MS.gene68828:CDS 20.0%
!!! TCGTTTAAGAAATGTATTTT+GGG - chr6.1:80151526-80151545 MS.gene68828:intron 20.0%
!!! TGATTTTGGTTCATTTAATA+CGG - chr6.1:80152225-80152244 MS.gene68828:CDS 20.0%
!!! TGTGGTTTTGATAATTTAAT+TGG - chr6.1:80152634-80152653 MS.gene68828:CDS 20.0%
! AAAATTCCAAGAATTTCAAG+TGG - chr6.1:80152309-80152328 MS.gene68828:CDS 25.0%
! AAACACTATGTCTTTAATTG+TGG + chr6.1:80152428-80152447 None:intergenic 25.0%
! AATTAAAGACATAGTGTTTG+AGG - chr6.1:80152429-80152448 MS.gene68828:CDS 25.0%
! ACTAAACTTAGTAGCAAAAA+TGG - chr6.1:80151017-80151036 MS.gene68828:intron 25.0%
! ATTGAAAGATAAAGTTGAGA+AGG - chr6.1:80152192-80152211 MS.gene68828:CDS 25.0%
! ATTGTCTTGATCAGTTATTA+TGG + chr6.1:80151780-80151799 None:intergenic 25.0%
! ATTTGATGAAAGAAATCGTT+GGG - chr6.1:80152033-80152052 MS.gene68828:CDS 25.0%
! CATCTTCTTCTATAATTTCA+GGG + chr6.1:80152804-80152823 None:intergenic 25.0%
! TAAAATGGAACCTAAATAAC+AGG + chr6.1:80153400-80153419 None:intergenic 25.0%
! TATTTGATGAAAGAAATCGT+TGG - chr6.1:80152032-80152051 MS.gene68828:CDS 25.0%
! TTTGAGTAAAAGTGATTTCA+TGG - chr6.1:80151949-80151968 MS.gene68828:CDS 25.0%
! TTTGTGGCATATAATGAAAA+AGG - chr6.1:80151853-80151872 MS.gene68828:CDS 25.0%
!! AAATTCTTGGAATTTTGCAT+TGG + chr6.1:80152305-80152324 None:intergenic 25.0%
!! AAGTATGTTTCCTGTTATTT+AGG - chr6.1:80153387-80153406 MS.gene68828:CDS 25.0%
!! AGTATTGAATTTGGATTGAA+GGG + chr6.1:80153288-80153307 None:intergenic 25.0%
!! GTCGTTTAAGAAATGTATTT+TGG - chr6.1:80151525-80151544 MS.gene68828:intron 25.0%
!! TGTTGTTTGTTTCAATTCAA+AGG - chr6.1:80152490-80152509 MS.gene68828:CDS 25.0%
!!! AAGTATTGAATTTGGATTGA+AGG + chr6.1:80153289-80153308 None:intergenic 25.0%
!!! AATTTTCAATATTTGATGGC+AGG - chr6.1:80153045-80153064 MS.gene68828:CDS 25.0%
!!! ATTACATGTATGTTTTTAGG+GGG - chr6.1:80153212-80153231 MS.gene68828:CDS 25.0%
!!! CTGTCATTTTTTCTTTTTGA+AGG - chr6.1:80153532-80153551 MS.gene68828:CDS 25.0%
!!! TAAAATAGCGTGTTTTGTTA+GGG + chr6.1:80151232-80151251 None:intergenic 25.0%
!!! TCACTCATAAGTATTGAATT+TGG + chr6.1:80153297-80153316 None:intergenic 25.0%
AAAAAAAGAAGCCAAGTAAG+TGG - chr6.1:80152952-80152971 MS.gene68828:CDS 30.0%
AAAGTGATTTCATGGAAAAG+TGG - chr6.1:80151957-80151976 MS.gene68828:CDS 30.0%
AAATCATCAAAGTCAACAAG+AGG - chr6.1:80152975-80152994 MS.gene68828:CDS 30.0%
AAGTGATTTCATGGAAAAGT+GGG - chr6.1:80151958-80151977 MS.gene68828:CDS 30.0%
AATGCGTGTATTTCTTTAGA+CGG - chr6.1:80151158-80151177 MS.gene68828:intron 30.0%
ACTTATGAGTGAAGTATATC+AGG - chr6.1:80153305-80153324 MS.gene68828:CDS 30.0%
AGTATGAACTCATTCTTTGA+TGG - chr6.1:80152115-80152134 MS.gene68828:CDS 30.0%
ATCCACAATAATAATGTTCC+AGG + chr6.1:80153361-80153380 None:intergenic 30.0%
CCATCTTCTTCTATAATTTC+AGG + chr6.1:80152805-80152824 None:intergenic 30.0%
CCTGAAATTATAGAAGAAGA+TGG - chr6.1:80152802-80152821 MS.gene68828:CDS 30.0%
CTAAATATAGTTCCAACCTT+AGG + chr6.1:80150936-80150955 None:intergenic 30.0%
GGAACTATATAGATAAAGTG+CGG - chr6.1:80151396-80151415 MS.gene68828:intron 30.0%
TAAAGTTGAGAAGGAAAACA+TGG - chr6.1:80152201-80152220 MS.gene68828:CDS 30.0%
TATAACAAAAATCCAGTGCA+AGG - chr6.1:80151127-80151146 MS.gene68828:intron 30.0%
TGAATAAACTTGCGAAAAAG+AGG - chr6.1:80152926-80152945 MS.gene68828:CDS 30.0%
TGATCAGTTATTATGGCATT+TGG + chr6.1:80151773-80151792 None:intergenic 30.0%
TTCATGACTTGAGGATAAAA+TGG + chr6.1:80153415-80153434 None:intergenic 30.0%
TTCCTGGAACATTATTATTG+TGG - chr6.1:80153356-80153375 MS.gene68828:CDS 30.0%
! ATATGTGAGAGACACATTTT+GGG - chr6.1:80152063-80152082 MS.gene68828:CDS 30.0%
! GAATATGACATTTGGTGAAA+AGG - chr6.1:80151367-80151386 MS.gene68828:intron 30.0%
! GATTGAAGATTTTGAACTTC+AGG - chr6.1:80151988-80152007 MS.gene68828:CDS 30.0%
! TTTGATGATTTCCACTTACT+TGG + chr6.1:80152966-80152985 None:intergenic 30.0%
!! ATAGCTTGTGTAAGTCATTT+TGG - chr6.1:80152250-80152269 MS.gene68828:CDS 30.0%
!! CTAAAATAGCGTGTTTTGTT+AGG + chr6.1:80151233-80151252 None:intergenic 30.0%
!! GTTCAAGTAGTTGATAAAGT+TGG - chr6.1:80153097-80153116 MS.gene68828:CDS 30.0%
!! TTGCACTGGATTTTTGTTAT+AGG + chr6.1:80151128-80151147 None:intergenic 30.0%
!!! ACTTACTTGGCTTCTTTTTT+TGG + chr6.1:80152953-80152972 None:intergenic 30.0%
!!! TTACATGTATGTTTTTAGGG+GGG - chr6.1:80153213-80153232 MS.gene68828:CDS 30.0%
AAAAAGGTTCCAGAAAAGCT+AGG - chr6.1:80151869-80151888 MS.gene68828:CDS 35.0%
AGGATACTAAGACCTTTACT+TGG - chr6.1:80151708-80151727 MS.gene68828:CDS 35.0%
AGGCATACCCTTATTAAATG+TGG - chr6.1:80152616-80152635 MS.gene68828:CDS 35.0%
AGTAGCAAAAATGGAGATGA+TGG - chr6.1:80151026-80151045 MS.gene68828:intron 35.0%
AGTATTACAAGAGTTATGCC+CGG - chr6.1:80150976-80150995 MS.gene68828:intron 35.0%
ATTGCTCTTGAGCATAATCA+TGG - chr6.1:80151200-80151219 MS.gene68828:intron 35.0%
CTATAGTTGTTGAAGCGAAT+GGG - chr6.1:80151339-80151358 MS.gene68828:intron 35.0%
CTCAAAGTCTTTCTCATTGA+AGG + chr6.1:80152458-80152477 None:intergenic 35.0%
GGGTATGAGAATATGACATT+TGG - chr6.1:80151359-80151378 MS.gene68828:intron 35.0%
TAACTCCAAAACCTATAGAC+CGG + chr6.1:80150998-80151017 None:intergenic 35.0%
TATCCTCAAGTCATGAATCA+TGG - chr6.1:80153418-80153437 MS.gene68828:CDS 35.0%
TCATGCTTGCTTTGATAGAT+TGG - chr6.1:80152354-80152373 MS.gene68828:CDS 35.0%
TCGCTTCAACAACTATAGAT+CGG + chr6.1:80151337-80151356 None:intergenic 35.0%
TCTATAGTTGTTGAAGCGAA+TGG - chr6.1:80151338-80151357 MS.gene68828:intron 35.0%
TGCCATGAAAAATGCAATTG+CGG - chr6.1:80151799-80151818 MS.gene68828:CDS 35.0%
TGCGAGAAAATATAAGAGGA+CGG + chr6.1:80152574-80152593 None:intergenic 35.0%
TGGTGATTTACTCCAACAAA+AGG + chr6.1:80151647-80151666 None:intergenic 35.0%
TTAGAACTCAATGATCAAGC+TGG - chr6.1:80151290-80151309 MS.gene68828:intron 35.0%
TTGAACTTCAGGATAACGAA+TGG - chr6.1:80151999-80152018 MS.gene68828:CDS 35.0%
TTTGTTGGAGTAAATCACCA+TGG - chr6.1:80151647-80151666 MS.gene68828:CDS 35.0%
! AGGGCTGCATATGAATATTT+TGG - chr6.1:80151563-80151582 MS.gene68828:intron 35.0%
! ATCCGCAATTGCATTTTTCA+TGG + chr6.1:80151804-80151823 None:intergenic 35.0%
! ATTCCAAGAATTTCAAGTGG+AGG - chr6.1:80152312-80152331 MS.gene68828:CDS 35.0%
! CACAATTTGAGCAACTTTTG+AGG + chr6.1:80152908-80152927 None:intergenic 35.0%
! CATATGTGAGAGACACATTT+TGG - chr6.1:80152062-80152081 MS.gene68828:CDS 35.0%
! CTTTTACGAAGTTGCTTCTA+CGG - chr6.1:80152696-80152715 MS.gene68828:CDS 35.0%
! TTTGAGGACTTCCTTTTTGA+AGG + chr6.1:80152892-80152911 None:intergenic 35.0%
! TTTTGGATCCTCGTTTGAAA+AGG - chr6.1:80151264-80151283 MS.gene68828:CDS 35.0%
!! AAGGAAAACATGGCTGATTT+TGG - chr6.1:80152211-80152230 MS.gene68828:CDS 35.0%
!!! AGTTTTAGGAGGAATGAACA+TGG - chr6.1:80153243-80153262 MS.gene68828:CDS 35.0%
AACTCCAAAACCTATAGACC+GGG + chr6.1:80150997-80151016 None:intergenic 40.0%
AATGTGTCTCTCACATATGC+AGG + chr6.1:80152061-80152080 None:intergenic 40.0%
ACACCATGATTCATGACTTG+AGG + chr6.1:80153424-80153443 None:intergenic 40.0%
ACTCCAACAAAAGGAGCAAA+TGG + chr6.1:80151638-80151657 None:intergenic 40.0%
AGGATAACGAATGGTTGAAG+GGG - chr6.1:80152008-80152027 MS.gene68828:CDS 40.0%
CAACCTCCACTTGAAATTCT+TGG + chr6.1:80152318-80152337 None:intergenic 40.0%
CAGGATAACGAATGGTTGAA+GGG - chr6.1:80152007-80152026 MS.gene68828:CDS 40.0%
CCACTGCGAGAAAATATAAG+AGG + chr6.1:80152578-80152597 None:intergenic 40.0%
CGTAGAAGCAACTTCGTAAA+AGG + chr6.1:80152698-80152717 None:intergenic 40.0%
CTTGCAATTTAGATGCACAC+AGG + chr6.1:80153072-80153091 None:intergenic 40.0%
CTTTGTGTGCTTAAGCTAAC+AGG - chr6.1:80152532-80152551 MS.gene68828:CDS 40.0%
GCTTGCTTTGATAGATTGGA+AGG - chr6.1:80152358-80152377 MS.gene68828:CDS 40.0%
TCAGGATAACGAATGGTTGA+AGG - chr6.1:80152006-80152025 MS.gene68828:CDS 40.0%
TGTTGTGAACAGCCAAGTAA+AGG + chr6.1:80151723-80151742 None:intergenic 40.0%
TTACTTGGCTGTTCACAACA+TGG - chr6.1:80151723-80151742 MS.gene68828:CDS 40.0%
TTTACTCTAGCATGTAGTCG+TGG - chr6.1:80151059-80151078 MS.gene68828:intron 40.0%
TTTAGGAGGAATGAACATGG+TGG - chr6.1:80153246-80153265 MS.gene68828:CDS 40.0%
! CATGGTCAATCTGTGTTGTT+GGG - chr6.1:80151665-80151684 MS.gene68828:CDS 40.0%
! CATTTGGTGAAAAGGACTGT+AGG - chr6.1:80151375-80151394 MS.gene68828:intron 40.0%
! CCTCTTATATTTTCTCGCAG+TGG - chr6.1:80152575-80152594 MS.gene68828:CDS 40.0%
! CTGTTAGCTTAAGCACACAA+AGG + chr6.1:80152534-80152553 None:intergenic 40.0%
! CTTATATTTTCTCGCAGTGG+AGG - chr6.1:80152578-80152597 MS.gene68828:CDS 40.0%
! TCTAACCTCCTTTTCAAACG+AGG + chr6.1:80151275-80151294 None:intergenic 40.0%
! TTGCATTGGTGAATGCTTTC+TGG + chr6.1:80152291-80152310 None:intergenic 40.0%
!! ATGCCATTTGCTCCTTTTGT+TGG - chr6.1:80151632-80151651 MS.gene68828:CDS 40.0%
!! TGGTGAATGCTTTCTGGAAT+TGG + chr6.1:80152285-80152304 None:intergenic 40.0%
AAGAGTTATGCCCGGTCTAT+AGG - chr6.1:80150984-80151003 MS.gene68828:intron 45.0%
AAGGTCGTCCTCCTTCAAAA+AGG - chr6.1:80152878-80152897 MS.gene68828:CDS 45.0%
ACATGGTTGGAATGCATGCA+TGG - chr6.1:80151740-80151759 MS.gene68828:CDS 45.0%
ATGTGCTTTACTGTCAAGCG+AGG - chr6.1:80151688-80151707 MS.gene68828:CDS 45.0%
CCAACAACACAGATTGACCA+TGG + chr6.1:80151667-80151686 None:intergenic 45.0%
CCATGGTCAATCTGTGTTGT+TGG - chr6.1:80151664-80151683 MS.gene68828:CDS 45.0%
CTTTACTTCGAGTCGGTACA+GGG + chr6.1:80152861-80152880 None:intergenic 45.0%
TATGCCACAAACACCATCGA+TGG + chr6.1:80151844-80151863 None:intergenic 45.0%
TGGATCCTCGTTTGAAAAGG+AGG - chr6.1:80151267-80151286 MS.gene68828:CDS 45.0%
TTCAGGGCATGTTTCTTGAG+AGG + chr6.1:80152788-80152807 None:intergenic 45.0%
TTGGCTGTTCACAACATGGT+TGG - chr6.1:80151727-80151746 MS.gene68828:CDS 45.0%
! TATGCCCGGTCTATAGGTTT+TGG - chr6.1:80150990-80151009 MS.gene68828:intron 45.0%
! TATTTTCTCGCAGTGGAGGA+AGG - chr6.1:80152582-80152601 MS.gene68828:CDS 45.0%
! TCCAGGAAACTAAAGAGCAC+TGG + chr6.1:80153344-80153363 None:intergenic 45.0%
! TCCAGTGCTCTTTAGTTTCC+TGG - chr6.1:80153340-80153359 MS.gene68828:CDS 45.0%
! TGTGAGAGACACATTTTGGG+CGG - chr6.1:80152066-80152085 MS.gene68828:CDS 45.0%
!! CATTCAATCTAGCCTTGCAC+TGG + chr6.1:80151142-80151161 None:intergenic 45.0%
!! CGTGTTGTTGATGATGACGA+TGG + chr6.1:80153578-80153597 None:intergenic 45.0%
!! GAGGACTTCCTTTTTGAAGG+AGG + chr6.1:80152889-80152908 None:intergenic 45.0%
!! GAGTGTCTACCTAGCTTTTC+TGG + chr6.1:80151881-80151900 None:intergenic 45.0%
!!! AGGGGGGACACATTAGTTTT+AGG - chr6.1:80153229-80153248 MS.gene68828:CDS 45.0%
!!! GGGGACACATTAGTTTTAGG+AGG - chr6.1:80153232-80153251 MS.gene68828:CDS 45.0%
CCTTTACTTCGAGTCGGTAC+AGG + chr6.1:80152862-80152881 None:intergenic 50.0%
CGCAGTGGAGGAAGGATATA+AGG - chr6.1:80152590-80152609 MS.gene68828:CDS 50.0%
GGACGACCTTTACTTCGAGT+CGG + chr6.1:80152868-80152887 None:intergenic 50.0%
GGAGGAAGGATATAAGGCGA+AGG - chr6.1:80152596-80152615 MS.gene68828:CDS 50.0%
TTCCAAAAGCTCGCCATCGA+TGG - chr6.1:80151828-80151847 MS.gene68828:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG - chr6.1:80151544-80151563 MS.gene68828:intron 50.0%
TTTGGGCAGATGCGAGATGT+AGG - chr6.1:80151543-80151562 MS.gene68828:intron 50.0%
! GTGAGAGACACATTTTGGGC+GGG - chr6.1:80152067-80152086 MS.gene68828:CDS 50.0%
!! CCTGTACCGACTCGAAGTAA+AGG - chr6.1:80152859-80152878 MS.gene68828:CDS 50.0%
AAAGTGCGGAGACTGCGACT+TGG - chr6.1:80151410-80151429 MS.gene68828:CDS 55.0%
AAGTGCGGAGACTGCGACTT+GGG - chr6.1:80151411-80151430 MS.gene68828:CDS 55.0%
ACTTGGGGTTGGAGATGCTG+AGG - chr6.1:80151427-80151446 MS.gene68828:CDS 55.0%
CTCGCCATCGATGGTGTTTG+TGG - chr6.1:80151837-80151856 MS.gene68828:CDS 55.0%
! CACCATCGATGGCGAGCTTT+TGG + chr6.1:80151833-80151852 None:intergenic 55.0%
AGTGCGGAGACTGCGACTTG+GGG - chr6.1:80151412-80151431 MS.gene68828:CDS 60.0%
! CGGAGACTGCGACTTGGGGT+TGG - chr6.1:80151416-80151435 MS.gene68828:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 80150930 80153607 80150930 ID=MS.gene68828
chr6.1 mRNA 80150930 80153607 80150930 ID=MS.gene68828.t1;Parent=MS.gene68828
chr6.1 exon 80151571 80153607 80151571 ID=MS.gene68828.t1.exon1;Parent=MS.gene68828.t1
chr6.1 CDS 80151571 80153607 80151571 ID=cds.MS.gene68828.t1;Parent=MS.gene68828.t1
chr6.1 exon 80151399 80151470 80151399 ID=MS.gene68828.t1.exon2;Parent=MS.gene68828.t1
chr6.1 CDS 80151399 80151470 80151399 ID=cds.MS.gene68828.t1;Parent=MS.gene68828.t1
chr6.1 exon 80151211 80151306 80151211 ID=MS.gene68828.t1.exon3;Parent=MS.gene68828.t1
chr6.1 CDS 80151211 80151306 80151211 ID=cds.MS.gene68828.t1;Parent=MS.gene68828.t1
chr6.1 exon 80151072 80151128 80151072 ID=MS.gene68828.t1.exon4;Parent=MS.gene68828.t1
chr6.1 CDS 80151072 80151128 80151072 ID=cds.MS.gene68828.t1;Parent=MS.gene68828.t1
chr6.1 exon 80150930 80150983 80150930 ID=MS.gene68828.t1.exon5;Parent=MS.gene68828.t1
chr6.1 CDS 80150930 80150983 80150930 ID=cds.MS.gene68828.t1;Parent=MS.gene68828.t1
Gene Sequence

>MS.gene68828

ATGCCTAAGGTTGGAACTATATTTAGCTCTAAAGAAGAACTTGAAGAGTATTACAAGAGTTATGCCCGGTCTATAGGTTTTGGAGTTACTAAACTTAGTAGCAAAAATGGAGATGATGGAAAAAAGTATTTTACTCTAGCATGTAGTCGTGGAACAAAGTATGTGAGTAAATCAAAGAAGCTTTTGAAGTCAAATCCTATAACAAAAATCCAGTGCAAGGCTAGATTGAATGCGTGTATTTCTTTAGACGGAACAGTTACTATTTCAAAAATTGCTCTTGAGCATAATCATGGTCTAAGCCCTAACAAAACACGCTATTTTAGAAGTAATAAGATTTTGGATCCTCGTTTGAAAAGGAGGTTAGAACTCAATGATCAAGCTGGAATAAATGTGAGCAGAAATTTCCGATCTATAGTTGTTGAAGCGAATGGGTATGAGAATATGACATTTGGTGAAAAGGACTGTAGGAACTATATAGATAAAGTGCGGAGACTGCGACTTGGGGTTGGAGATGCTGAGGCGATTCAAAATTATTTTGTTAAAATGCAAAAGCAAAATAGTCAATTCTATTATATAATAGACATTGATGATAAAAGTCGTTTAAGAAATGTATTTTGGGCAGATGCGAGATGTAGGGCTGCATATGAATATTTTGGTGATGTCGTAACATTTGACACTACTTACTTAACAAATAAATATGACATGCCATTTGCTCCTTTTGTTGGAGTAAATCACCATGGTCAATCTGTGTTGTTGGGATGTGCTTTACTGTCAAGCGAGGATACTAAGACCTTTACTTGGCTGTTCACAACATGGTTGGAATGCATGCATGGACGTGCTCCAAATGCCATAATAACTGATCAAGACAATGCCATGAAAAATGCAATTGCGGATGTTTTTCCAAAAGCTCGCCATCGATGGTGTTTGTGGCATATAATGAAAAAGGTTCCAGAAAAGCTAGGTAGACACTCTCATTATGAGTCTATCAAAGAACTTTTGCATGATGTTGTATACGATTCTTTGAGTAAAAGTGATTTCATGGAAAAGTGGGAAAGAATGATTGAAGATTTTGAACTTCAGGATAACGAATGGTTGAAGGGGCTATTTGATGAAAGAAATCGTTGGGTTCCTGCATATGTGAGAGACACATTTTGGGCGGGCATGTCAACGACTCAAAGAAGTGAAAGTATGAACTCATTCTTTGATGGTTATGTAAATTTAAAGACAACATTGAAGCAATTTGTTGAACAATATGACAATGCATTGAAAGATAAAGTTGAGAAGGAAAACATGGCTGATTTTGGTTCATTTAATACGGTGATAGCTTGTGTAAGTCATTTTGGCTTTGAGTTCCAATTCCAGAAAGCATTCACCAATGCAAAATTCCAAGAATTTCAAGTGGAGGTTGCTTCTATGATTTATTGTCATGCTTGCTTTGATAGATTGGAAGGATCGAATTCTATATATTCTGTTATAGAAAGCAAGAAAAAATTTGCCACAATTAAAGACATAGTGTTTGAGGTTTCCTTCAATGAGAAAGACTTTGAGTTACAATGTATGTGTTGTTTGTTTCAATTCAAAGGTATTTTATGCAGACACATCCTTTGTGTGCTTAAGCTAACAGGAAAAACAGAGTCATTGCCGTCCTCTTATATTTTCTCGCAGTGGAGGAAGGATATAAGGCGAAGGCATACCCTTATTAAATGTGGTTTTGATAATTTAATTGGAAATGCTGAATTGCAAAGAGTTGATAAAGCTTGTTCTGCCTTTTACGAAGTTGCTTCTACGGTGATAAATAACGATAATGATTTGTCGAAAATGATGAATCTAATCAAGAAAATAAAAAGTGAGTTTACCTCTCAAGAAACATGCCCTGAAATTATAGAAGAAGATGGTTTAGTTCATAATCAAGTGACTAAAATTCTTGACCCTGTACCGACTCGAAGTAAAGGTCGTCCTCCTTCAAAAAGGAAGTCCTCAAAAGTTGCTCAAATTGTGAATAAACTTGCGAAAAAGAGGACCAAAAAAAGAAGCCAAGTAAGTGGAAATCATCAAAGTCAACAAGAGGTAATTTAAGATACGTGTACATCTAAATTAATAATTTTGAATATGTCTAATTTTCAATATTTGATGGCAGGACCTGTGTGCATCTAAATTGCAAGATAGTGTTCAAGTAGTTGATAAAGTTGGCACACAAGAGAGCATTCAAGTAAGTAATTTTATTTATTCTCAGCTTTAGTTTTAAACAATCAATGAGAACTACATACTGATTGTCTATGTTTATTACATGTATGTTTTTAGGGGGGACACATTAGTTTTAGGAGGAATGAACATGGTGGAGTAAATAATTTAGCTCCCTTCAATCCAAATTCAATACTTATGAGTGAAGTATATCAGGTGCAAGCATAAATCCAGTGCTCTTTAGTTTCCTGGAACATTATTATTGTGGATTCTAACAAGTATGTTTCCTGTTATTTAGGTTCCATTTTATCCTCAAGTCATGAATCATGGTGTTTCATATTTTGAATTATTACAGGTACTTGATCTTTATCGTCCTCTTATGTTTCTTTTAATTAAATATTTTGATATAAATTCATCAACTCTGTCATTTTTTCTTTTTGAAGGCACAACATCATGTGAATGATCCATCGTCATCATCAACAACACGTGAATCATAA
Protein sequence

>MS.gene68828.t1

MPKVGTIFSSKEELEEYYKSYARSIGFGVTKLSSKNGDDGKKYFTLACSRGTKYVSKSKKLLKSNPITKIQCKARLNACISLDGTVTISKIALEHNHGLSPNKTRYFRSNKILDPRLKRRLELNDQAGINVSRNFRSIVVEANGYENMTFGEKDCRNYIDKVRRLRLGVGDAEAIQNYFVKMQKQNSQFYYIIDIDDKSRLRNVFWADARCRAAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSSEDTKTFTWLFTTWLECMHGRAPNAIITDQDNAMKNAIADVFPKARHRWCLWHIMKKVPEKLGRHSHYESIKELLHDVVYDSLSKSDFMEKWERMIEDFELQDNEWLKGLFDERNRWVPAYVRDTFWAGMSTTQRSESMNSFFDGYVNLKTTLKQFVEQYDNALKDKVEKENMADFGSFNTVIACVSHFGFEFQFQKAFTNAKFQEFQVEVASMIYCHACFDRLEGSNSIYSVIESKKKFATIKDIVFEVSFNEKDFELQCMCCLFQFKGILCRHILCVLKLTGKTESLPSSYIFSQWRKDIRRRHTLIKCGFDNLIGNAELQRVDKACSAFYEVASTVINNDNDLSKMMNLIKKIKSEFTSQETCPEIIEEDGLVHNQVTKILDPVPTRSKGRPPSKRKSSKVAQIVNKLAKKRTKKRSQDLCASKLQDSVQVVDKVGTQESIQGGHISFRRNEHGGVNNLAPFNPNSILMSEVYQVPFYPQVMNHGVSYFELLQAQHHVNDPSSSSTTRES