Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68869.t1 | XP_003610485.1 | 97.1 | 140 | 4 | 0 | 1 | 140 | 1 | 140 | 2.00E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68869.t1 | P0DKG3 | 54.5 | 112 | 40 | 1 | 40 | 140 | 296 | 407 | 2.0e-23 | 110.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68869.t1 | G7JKF5 | 97.1 | 140 | 4 | 0 | 1 | 140 | 1 | 140 | 1.5e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene68869.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68869.t1 | MTR_4g132740 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 1.10e-99 | 282 |
| MS.gene68869.t1 | MTR_4g028930 | 50.000 | 62 | 31 | 0 | 79 | 140 | 312 | 373 | 6.22e-15 | 70.5 |
| MS.gene68869.t1 | MTR_7g072875 | 50.000 | 58 | 29 | 0 | 83 | 140 | 221 | 278 | 1.30e-13 | 66.2 |
| MS.gene68869.t1 | MTR_7g072630 | 50.000 | 58 | 29 | 0 | 83 | 140 | 283 | 340 | 2.48e-13 | 65.9 |
| MS.gene68869.t1 | MTR_5g075210 | 44.776 | 67 | 37 | 0 | 74 | 140 | 273 | 339 | 4.38e-13 | 65.1 |
| MS.gene68869.t1 | MTR_2g018030 | 41.379 | 58 | 34 | 0 | 83 | 140 | 279 | 336 | 7.94e-12 | 61.6 |
| MS.gene68869.t1 | MTR_0907s0020 | 46.552 | 58 | 31 | 0 | 83 | 140 | 104 | 161 | 5.21e-11 | 57.8 |
| MS.gene68869.t1 | MTR_8g087530 | 41.379 | 58 | 34 | 0 | 83 | 140 | 260 | 317 | 6.59e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68869.t1 | AT4G04025 | 54.305 | 151 | 55 | 3 | 4 | 140 | 22 | 172 | 2.60e-45 | 145 |
| MS.gene68869.t1 | AT1G06920 | 41.860 | 86 | 45 | 2 | 58 | 138 | 215 | 300 | 3.29e-13 | 65.5 |
| MS.gene68869.t1 | AT2G30400 | 48.387 | 62 | 28 | 1 | 79 | 140 | 254 | 311 | 8.47e-13 | 64.3 |
| MS.gene68869.t1 | AT5G58360 | 39.080 | 87 | 41 | 3 | 60 | 138 | 199 | 281 | 8.53e-13 | 63.9 |
| MS.gene68869.t1 | AT5G01840 | 48.148 | 54 | 28 | 0 | 87 | 140 | 204 | 257 | 1.59e-12 | 63.2 |
| MS.gene68869.t1 | AT4G18830 | 43.103 | 58 | 33 | 0 | 83 | 140 | 284 | 341 | 2.68e-12 | 63.2 |
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGACATGTAGAGAGAAAA+AGG | 0.217537 | 8.2:-1526729 | MS.gene68869:CDS |
| ATGATCCAAGAGAGGATTTC+AGG | 0.230726 | 8.2:-1526648 | MS.gene68869:CDS |
| GTTCTTCTTCTGTCTCTGAT+AGG | 0.352483 | 8.2:-1526828 | MS.gene68869:CDS |
| GAAGATCTTTCGCATCTTGA+AGG | 0.366336 | 8.2:+1526593 | None:intergenic |
| AAATCTCAAGTATAAGACTA+TGG | 0.438492 | 8.2:+1526527 | None:intergenic |
| AAAAGGGAGAAGAGGAGAGA+AGG | 0.442296 | 8.2:-1526712 | MS.gene68869:CDS |
| GACTCCCTGAAATCCTCTCT+TGG | 0.457320 | 8.2:+1526643 | None:intergenic |
| GGATTTCAGGGAGTCTATGA+TGG | 0.485788 | 8.2:-1526635 | MS.gene68869:CDS |
| CTATTATATGTCGATGAACT+CGG | 0.518436 | 8.2:-1526557 | MS.gene68869:CDS |
| TGATCCAAGAGAGGATTTCA+GGG | 0.524133 | 8.2:-1526647 | MS.gene68869:CDS |
| ATATAGTGTTTCTTCTTCAG+AGG | 0.569609 | 8.2:-1526860 | MS.gene68869:CDS |
| GTCAAGTCTCTCTTGCACCA+TGG | 0.576800 | 8.2:+1526777 | None:intergenic |
| AAAACAACAAAGAGGATGCA+AGG | 0.595456 | 8.2:-1526905 | MS.gene68869:CDS |
| AAAACCTCAAAACAACAAAG+AGG | 0.609556 | 8.2:-1526913 | MS.gene68869:CDS |
| TGTGCTAGTGTAGAAACAGA+AGG | 0.614020 | 8.2:+1526802 | None:intergenic |
| TTCTACACTAGCACATGCCA+TGG | 0.653332 | 8.2:-1526794 | MS.gene68869:CDS |
| GTGTTCTTATGATCCAAGAG+AGG | 0.658417 | 8.2:-1526656 | MS.gene68869:CDS |
| GTGCTAGTGTAGAAACAGAA+GGG | 0.663929 | 8.2:+1526803 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCTCAAGTATAAGACTA+TGG | + | chr8.2:1526911-1526930 | None:intergenic | 25.0% |
| !! | CAAGTTTTTCTCTTATCATT+TGG | + | chr8.2:1526683-1526702 | None:intergenic | 25.0% |
| AAAACCTCAAAACAACAAAG+AGG | - | chr8.2:1526522-1526541 | MS.gene68869:CDS | 30.0% | |
| ATATAGTGTTTCTTCTTCAG+AGG | - | chr8.2:1526575-1526594 | MS.gene68869:CDS | 30.0% | |
| CTATTATATGTCGATGAACT+CGG | - | chr8.2:1526878-1526897 | MS.gene68869:CDS | 30.0% | |
| TAGACATGTAGAGAGAAAAA+GGG | - | chr8.2:1526707-1526726 | MS.gene68869:CDS | 30.0% | |
| AAAACAACAAAGAGGATGCA+AGG | - | chr8.2:1526530-1526549 | MS.gene68869:CDS | 35.0% | |
| CTAGACATGTAGAGAGAAAA+AGG | - | chr8.2:1526706-1526725 | MS.gene68869:CDS | 35.0% | |
| ATGATCCAAGAGAGGATTTC+AGG | - | chr8.2:1526787-1526806 | MS.gene68869:CDS | 40.0% | |
| GAAGATCTTTCGCATCTTGA+AGG | + | chr8.2:1526845-1526864 | None:intergenic | 40.0% | |
| GTGCTAGTGTAGAAACAGAA+GGG | + | chr8.2:1526635-1526654 | None:intergenic | 40.0% | |
| GTGTTCTTATGATCCAAGAG+AGG | - | chr8.2:1526779-1526798 | MS.gene68869:CDS | 40.0% | |
| GTTCTTCTTCTGTCTCTGAT+AGG | - | chr8.2:1526607-1526626 | MS.gene68869:CDS | 40.0% | |
| TAGAGAGAAAAAGGGAGAAG+AGG | - | chr8.2:1526715-1526734 | MS.gene68869:CDS | 40.0% | |
| TGATCCAAGAGAGGATTTCA+GGG | - | chr8.2:1526788-1526807 | MS.gene68869:CDS | 40.0% | |
| TGTGCTAGTGTAGAAACAGA+AGG | + | chr8.2:1526636-1526655 | None:intergenic | 40.0% | |
| !!! | GCATCCTCTTTGTTGTTTTG+AGG | + | chr8.2:1526529-1526548 | None:intergenic | 40.0% |
| AAAAGGGAGAAGAGGAGAGA+AGG | - | chr8.2:1526723-1526742 | MS.gene68869:CDS | 45.0% | |
| GGATTTCAGGGAGTCTATGA+TGG | - | chr8.2:1526800-1526819 | MS.gene68869:CDS | 45.0% | |
| TTCTACACTAGCACATGCCA+TGG | - | chr8.2:1526641-1526660 | MS.gene68869:CDS | 45.0% | |
| GACTCCCTGAAATCCTCTCT+TGG | + | chr8.2:1526795-1526814 | None:intergenic | 50.0% | |
| GTCAAGTCTCTCTTGCACCA+TGG | + | chr8.2:1526661-1526680 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 1526519 | 1526938 | 1526519 | ID=MS.gene68869 |
| chr8.2 | mRNA | 1526519 | 1526938 | 1526519 | ID=MS.gene68869.t1;Parent=MS.gene68869 |
| chr8.2 | exon | 1526519 | 1526938 | 1526519 | ID=MS.gene68869.t1.exon1;Parent=MS.gene68869.t1 |
| chr8.2 | CDS | 1526519 | 1526938 | 1526519 | ID=cds.MS.gene68869.t1;Parent=MS.gene68869.t1 |
| Gene Sequence |
| Protein sequence |
