AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68902

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68902.t1 MTR_2g436400 92.857 280 20 0 1 280 102 381 0.0 531
MS.gene68902.t1 MTR_2g437060 79.570 279 57 0 2 280 111 389 2.56e-161 454
MS.gene68902.t1 MTR_2g437080 70.357 280 83 0 1 280 95 374 1.19e-140 401
MS.gene68902.t1 MTR_2g437030 69.286 280 86 0 1 280 181 460 6.51e-134 387
MS.gene68902.t1 MTR_2g436440 67.143 280 92 0 1 280 101 380 3.66e-131 377
MS.gene68902.t1 MTR_2g437020 65.125 281 95 2 2 280 95 374 1.60e-126 365
MS.gene68902.t1 MTR_2g437040 63.571 280 102 0 1 280 95 374 2.27e-124 360
MS.gene68902.t1 MTR_4g119550 52.669 281 132 1 1 280 104 384 9.10e-105 310
MS.gene68902.t1 MTR_2g437960 51.237 283 135 2 1 280 108 390 2.43e-98 294
MS.gene68902.t1 MTR_2g437170 52.281 285 131 3 1 280 110 394 3.36e-98 294
MS.gene68902.t1 MTR_2g437960 51.237 283 135 2 1 280 108 390 6.11e-98 294
MS.gene68902.t1 MTR_2g438010 50.877 285 133 3 1 280 125 407 1.03e-96 291
MS.gene68902.t1 MTR_2g437150 52.143 280 129 3 1 275 110 389 3.15e-96 289
MS.gene68902.t1 MTR_2g437180 50.896 279 132 2 1 276 104 380 1.12e-95 287
MS.gene68902.t1 MTR_2g437120 52.688 279 128 2 1 276 106 383 6.45e-95 286
MS.gene68902.t1 MTR_2g437100 53.025 281 127 3 1 276 115 395 6.81e-95 286
MS.gene68902.t1 MTR_2g437160 51.064 282 136 1 1 280 118 399 1.16e-94 285
MS.gene68902.t1 MTR_2g437130 49.643 280 141 0 1 280 96 375 1.21e-92 279
MS.gene68902.t1 MTR_2g437990 48.582 282 143 1 1 280 105 386 1.73e-92 280
MS.gene68902.t1 MTR_2g437200 46.178 314 131 2 1 276 85 398 4.23e-92 278
MS.gene68902.t1 MTR_2g076320 48.780 287 134 2 2 280 106 387 1.72e-90 274
MS.gene68902.t1 MTR_2g437260 46.570 277 146 1 1 275 89 365 6.49e-89 270
MS.gene68902.t1 MTR_2g438020 45.541 314 133 2 1 276 124 437 8.12e-89 273
MS.gene68902.t1 MTR_2g437240 43.711 318 141 2 1 280 85 402 1.75e-88 270
MS.gene68902.t1 MTR_2g436460 46.570 277 147 1 1 276 100 376 9.82e-85 258
MS.gene68902.t1 MTR_2g019840 48.239 284 139 4 1 280 96 375 2.00e-83 255
MS.gene68902.t1 MTR_2g437940 47.857 280 121 3 1 280 104 358 2.88e-80 247
MS.gene68902.t1 MTR_2g019810 46.071 280 147 3 1 278 84 361 3.85e-79 244
MS.gene68902.t1 MTR_2g437090 69.753 162 49 0 119 280 1 162 1.87e-78 235
MS.gene68902.t1 MTR_4g120380 42.701 274 157 0 1 274 97 370 3.73e-72 226
MS.gene68902.t1 MTR_4g119580 41.606 274 160 0 1 274 97 370 5.68e-71 223
MS.gene68902.t1 MTR_4g120380 42.913 254 145 0 1 254 97 350 2.90e-66 213
MS.gene68902.t1 MTR_2g437160 54.082 196 87 1 88 280 113 308 8.15e-66 208
MS.gene68902.t1 MTR_4g119570 36.437 247 124 1 26 272 14 227 3.99e-47 157
MS.gene68902.t1 MTR_3g450500 32.509 283 181 4 2 276 92 372 5.67e-45 156
MS.gene68902.t1 MTR_2g436370 77.895 95 21 0 1 95 101 195 1.12e-41 142
MS.gene68902.t1 MTR_4g007490 30.070 286 189 4 1 278 90 372 2.74e-36 133
MS.gene68902.t1 MTR_2g436380 55.752 113 43 3 119 231 1 106 1.71e-30 115
MS.gene68902.t1 MTR_8g080990 28.889 270 188 3 4 270 100 368 3.99e-30 117
MS.gene68902.t1 MTR_8g081000 25.899 278 201 3 1 274 109 385 6.49e-27 108
MS.gene68902.t1 MTR_8g081000 25.899 278 201 3 1 274 109 385 6.60e-27 108
MS.gene68902.t1 MTR_8g081000 25.899 278 201 3 1 274 109 385 8.42e-27 108
MS.gene68902.t1 MTR_3g085240 60.377 53 21 0 178 230 176 228 1.14e-15 75.1
MS.gene68902.t1 MTR_4g119615 60.000 50 20 0 218 267 26 75 3.15e-14 67.4
MS.gene68902.t1 MTR_2g019830 53.846 65 29 1 212 276 136 199 3.56e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68902.t1 AT5G07900 35.842 279 173 3 1 273 121 399 6.71e-55 182
MS.gene68902.t1 AT1G21150 29.930 284 191 4 1 277 72 354 8.29e-36 131
MS.gene68902.t1 AT1G21150 29.930 284 191 4 1 277 108 390 1.52e-35 131
MS.gene68902.t1 AT1G21150 29.930 284 191 4 1 277 172 454 3.06e-35 132
MS.gene68902.t1 AT1G61980 26.861 309 175 6 1 265 104 405 2.14e-26 107
MS.gene68902.t1 AT1G61980 26.861 309 175 6 1 265 104 405 2.14e-26 107
MS.gene68902.t1 AT1G61980 26.861 309 175 6 1 265 104 405 2.14e-26 107
MS.gene68902.t1 AT5G64950 30.175 285 179 6 6 274 96 376 2.13e-24 101
MS.gene68902.t1 AT1G62010 25.442 283 172 5 20 265 109 389 2.59e-24 101
MS.gene68902.t1 AT3G46950 24.928 349 186 8 1 277 104 448 2.28e-23 99.4
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.28e-22 95.5
MS.gene68902.t1 AT1G62120 24.510 306 188 6 1 265 108 411 1.08e-21 94.4
MS.gene68902.t1 AT1G62085 25.507 345 171 10 1 265 107 445 1.59e-20 91.3
MS.gene68902.t1 AT1G62085 25.507 345 171 10 1 265 107 445 1.59e-20 91.3
MS.gene68902.t1 AT1G61990 25.321 312 187 5 1 273 104 408 1.67e-20 90.9
MS.gene68902.t1 AT1G61990 25.321 312 187 5 1 273 104 408 1.67e-20 90.9
MS.gene68902.t1 AT1G61960 24.069 349 188 8 1 273 104 451 4.48e-17 81.3
MS.gene68902.t1 AT5G23930 23.284 335 181 8 1 264 107 436 1.67e-15 76.6
MS.gene68902.t1 AT1G62110 27.835 194 133 3 1 189 104 295 5.70e-15 75.1

Find 48 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTGACAAGGAAATTT+TGG 0.119034 2.4:-55019273 MS.gene68902:CDS
TAAATTTAGTGATGAACTTT+TGG 0.199642 2.4:-55018902 MS.gene68902:CDS
GTGAAGGAATTAAAGGATTT+GGG 0.230293 2.4:-55019098 MS.gene68902:CDS
AGACCAACCCCATTTCTTAA+AGG 0.251996 2.4:+55018989 None:intergenic
GGTATTGATTACTGTTGTTT+TGG 0.274832 2.4:+55019034 None:intergenic
TGACAAAATATAAGGTGTTT+TGG 0.274912 2.4:+55018845 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.282534 2.4:-55019099 MS.gene68902:CDS
ATGGATGTTAACATACAATA+AGG 0.292032 2.4:+55018933 None:intergenic
AGGTTGGGACTATTTGATTC+TGG 0.292547 2.4:+55019318 None:intergenic
AAACTTTGGCAACACCGTTT+TGG 0.327649 2.4:+55019424 None:intergenic
AAACTTACTGACAAAATATA+AGG 0.343939 2.4:+55018837 None:intergenic
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2.4:-55018998 MS.gene68902:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.365271 2.4:-55018997 MS.gene68902:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.4:-55018883 MS.gene68902:CDS
CAAGTCGGTGAAGGAATTAA+AGG 0.378802 2.4:-55019105 MS.gene68902:CDS
AACTTATCAGTCACTACAAA+TGG 0.416436 2.4:+55018729 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.4:+55019388 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2.4:-55019309 MS.gene68902:CDS
TATCAACTTTCTCTTTCCAT+AGG 0.439630 2.4:+55019012 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2.4:+55019449 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.457142 2.4:-55019197 MS.gene68902:CDS
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2.4:+55019457 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.478001 2.4:+55019298 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.4:-55018884 MS.gene68902:CDS
CTGTACACCAATTCATAGGT+TGG 0.484595 2.4:+55019302 None:intergenic
AGGCTTGCATACAAAACTCT+AGG 0.506576 2.4:+55019341 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.4:-55018874 MS.gene68902:CDS
TGTACACCAATTCATAGGTT+GGG 0.507173 2.4:+55019303 None:intergenic
TCACAATGCTGATTGAGAAT+GGG 0.508211 2.4:-55019196 MS.gene68902:CDS
CAACAGTAATCAATACCCTA+TGG 0.513109 2.4:-55019028 MS.gene68902:CDS
ATCAACTTTCTCTTTCCATA+GGG 0.529759 2.4:+55019013 None:intergenic
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2.4:-55018783 MS.gene68902:CDS
TTCCTGCAAACCCTCCAAAA+CGG 0.545667 2.4:-55019438 MS.gene68902:CDS
GTTGATACCTTTAAGAAATG+GGG 0.553181 2.4:-55018996 MS.gene68902:CDS
TTGGGACTATTTGATTCTGG+AGG 0.560239 2.4:+55019321 None:intergenic
AATTGCACAACAGCGGCCCT+CGG 0.562081 2.4:+55018798 None:intergenic
CAGATTCTTGCAATCTGACA+AGG 0.575407 2.4:-55019282 MS.gene68902:CDS
CATTGCTGAGGGAACAGACT+CGG 0.581919 2.4:-55019151 MS.gene68902:CDS
ATACCTTTAAGAAATGGGGT+TGG 0.587688 2.4:-55018992 MS.gene68902:CDS
TGAAGGAATTAAAGGATTTG+GGG 0.597102 2.4:-55019097 MS.gene68902:CDS
TGACATGCTCAAGTCGGTGA+AGG 0.598383 2.4:-55019114 MS.gene68902:CDS
AAACATTAAAACATTGCTGA+GGG 0.598387 2.4:-55019162 MS.gene68902:CDS
GGCTTGCATACAAAACTCTA+GGG 0.606205 2.4:+55019342 None:intergenic
GACAATGTTAACAATGTCAG+AGG 0.618058 2.4:+55019373 None:intergenic
CACAATGCTGATTGAGAATG+GGG 0.632348 2.4:-55019195 MS.gene68902:CDS
GAGAAGAAATTGCACAACAG+CGG 0.635644 2.4:+55018791 None:intergenic
CAAACATTAAAACATTGCTG+AGG 0.635961 2.4:-55019163 MS.gene68902:CDS
GAGACGTGACATGCTCAAGT+CGG 0.666473 2.4:-55019120 MS.gene68902:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTTATTTTTTCAA+TGG + chr2.4:55019197-55019216 None:intergenic 15.0%
!! AAACTTACTGACAAAATATA+AGG + chr2.4:55019274-55019293 None:intergenic 20.0%
!!! AAATTTAGTGATGAACTTTT+GGG - chr2.4:55019207-55019226 MS.gene68902:CDS 20.0%
!!! TAAATTTAGTGATGAACTTT+TGG - chr2.4:55019206-55019225 MS.gene68902:CDS 20.0%
!!! TTATATTTTGTCAGTAAGTT+TGG - chr2.4:55019273-55019292 MS.gene68902:CDS 20.0%
!!! TTATCTTTTAAAGTTGTATG+AGG - chr2.4:55019444-55019463 MS.gene68902:CDS 20.0%
! AAACATTAAAACATTGCTGA+GGG - chr2.4:55018946-55018965 MS.gene68902:CDS 25.0%
! ATGGATGTTAACATACAATA+AGG + chr2.4:55019178-55019197 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.4:55018701-55018720 None:intergenic 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.4:55019037-55019056 MS.gene68902:CDS 25.0%
!! AGTTGATACCTTTAAGAAAT+GGG - chr2.4:55019111-55019130 MS.gene68902:CDS 25.0%
!!! AAGTTGATACCTTTAAGAAA+TGG - chr2.4:55019110-55019129 MS.gene68902:CDS 25.0%
!!! TATAAAACGTTTTGAGAATG+AGG - chr2.4:55019417-55019436 MS.gene68902:CDS 25.0%
!!! TGACAAAATATAAGGTGTTT+TGG + chr2.4:55019266-55019285 None:intergenic 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.4:55018704-55018723 MS.gene68902:CDS 25.0%
AACTTATCAGTCACTACAAA+TGG + chr2.4:55019382-55019401 None:intergenic 30.0%
ATCAACTTTCTCTTTCCATA+GGG + chr2.4:55019098-55019117 None:intergenic 30.0%
CAAACATTAAAACATTGCTG+AGG - chr2.4:55018945-55018964 MS.gene68902:CDS 30.0%
GTGAAGGAATTAAAGGATTT+GGG - chr2.4:55019010-55019029 MS.gene68902:CDS 30.0%
TATCAACTTTCTCTTTCCAT+AGG + chr2.4:55019099-55019118 None:intergenic 30.0%
TGAAGGAATTAAAGGATTTG+GGG - chr2.4:55019011-55019030 MS.gene68902:CDS 30.0%
TGTCAGTAAGTTTGGAAAAA+AGG - chr2.4:55019281-55019300 MS.gene68902:CDS 30.0%
! GTTGATACCTTTAAGAAATG+GGG - chr2.4:55019112-55019131 MS.gene68902:CDS 30.0%
!!! AATATAAGGTGTTTTGGCAA+TGG + chr2.4:55019260-55019279 None:intergenic 30.0%
!!! GGTATTGATTACTGTTGTTT+TGG + chr2.4:55019077-55019096 None:intergenic 30.0%
AATAGTCCCAACCTATGAAT+TGG - chr2.4:55018799-55018818 MS.gene68902:CDS 35.0%
CAACAGTAATCAATACCCTA+TGG - chr2.4:55019080-55019099 MS.gene68902:CDS 35.0%
GAATCTGTACACCAATTCAT+AGG + chr2.4:55018813-55018832 None:intergenic 35.0%
GACAATGTTAACAATGTCAG+AGG + chr2.4:55018738-55018757 None:intergenic 35.0%
GGTGAAGGAATTAAAGGATT+TGG - chr2.4:55019009-55019028 MS.gene68902:CDS 35.0%
GTGCAATTTCTTCTCAACAA+AGG - chr2.4:55019325-55019344 MS.gene68902:CDS 35.0%
TATGAGGAAAAACGCAATCT+AGG - chr2.4:55019460-55019479 MS.gene68902:CDS 35.0%
TGTACACCAATTCATAGGTT+GGG + chr2.4:55018808-55018827 None:intergenic 35.0%
! ATACCTTTAAGAAATGGGGT+TGG - chr2.4:55019116-55019135 MS.gene68902:CDS 35.0%
! ATCACAATGCTGATTGAGAA+TGG - chr2.4:55018911-55018930 MS.gene68902:CDS 35.0%
! GCAATCTGACAAGGAAATTT+TGG - chr2.4:55018835-55018854 MS.gene68902:CDS 35.0%
! TCACAATGCTGATTGAGAAT+GGG - chr2.4:55018912-55018931 MS.gene68902:CDS 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG - chr2.4:55019220-55019239 MS.gene68902:CDS 35.0%
!!! GCACCAAAAGTTGTTTTTGA+AGG + chr2.4:55019043-55019062 None:intergenic 35.0%
AGACCAACCCCATTTCTTAA+AGG + chr2.4:55019122-55019141 None:intergenic 40.0%
AGGCTTGCATACAAAACTCT+AGG + chr2.4:55018770-55018789 None:intergenic 40.0%
AGGTTGGGACTATTTGATTC+TGG + chr2.4:55018793-55018812 None:intergenic 40.0%
CAAGTCGGTGAAGGAATTAA+AGG - chr2.4:55019003-55019022 MS.gene68902:CDS 40.0%
CAGATTCTTGCAATCTGACA+AGG - chr2.4:55018826-55018845 MS.gene68902:CDS 40.0%
CTGTACACCAATTCATAGGT+TGG + chr2.4:55018809-55018828 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+CGG + chr2.4:55019320-55019339 None:intergenic 40.0%
GGAAAAAAGGATCATTCCGA+GGG - chr2.4:55019294-55019313 MS.gene68902:CDS 40.0%
GGCTTGCATACAAAACTCTA+GGG + chr2.4:55018769-55018788 None:intergenic 40.0%
TGGAAAAAAGGATCATTCCG+AGG - chr2.4:55019293-55019312 MS.gene68902:CDS 40.0%
TTGGGACTATTTGATTCTGG+AGG + chr2.4:55018790-55018809 None:intergenic 40.0%
! AAACTTTGGCAACACCGTTT+TGG + chr2.4:55018687-55018706 None:intergenic 40.0%
! CACAATGCTGATTGAGAATG+GGG - chr2.4:55018913-55018932 MS.gene68902:CDS 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG - chr2.4:55019219-55019238 MS.gene68902:CDS 40.0%
TTCCTGCAAACCCTCCAAAA+CGG - chr2.4:55018670-55018689 MS.gene68902:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.4:55019225-55019244 MS.gene68902:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.4:55019224-55019243 MS.gene68902:CDS 45.0%
!! TTTGGCAACACCGTTTTGGA+GGG + chr2.4:55018683-55018702 None:intergenic 45.0%
CATTGCTGAGGGAACAGACT+CGG - chr2.4:55018957-55018976 MS.gene68902:CDS 50.0%
GAGACGTGACATGCTCAAGT+CGG - chr2.4:55018988-55019007 MS.gene68902:CDS 50.0%
TGACATGCTCAAGTCGGTGA+AGG - chr2.4:55018994-55019013 MS.gene68902:CDS 50.0%
! CTTTGGCAACACCGTTTTGG+AGG + chr2.4:55018684-55018703 None:intergenic 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.4:55018723-55018742 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.4:55019234-55019253 MS.gene68902:CDS 50.0%
AATTGCACAACAGCGGCCCT+CGG + chr2.4:55019313-55019332 None:intergenic 55.0%
GGTTTGCAGGAAAGCAGCTC+TGG + chr2.4:55018662-55018681 None:intergenic 55.0%
! GGAAAGCAGCTCTGGCATCT+TGG + chr2.4:55018654-55018673 None:intergenic 55.0%
!!! CACCGTTTTGGAGGGTTTGC+AGG + chr2.4:55018675-55018694 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 55018644 55019486 55018644 ID=MS.gene68902
chr2.4 mRNA 55018644 55019486 55018644 ID=MS.gene68902.t1;Parent=MS.gene68902
chr2.4 exon 55018644 55019486 55018644 ID=MS.gene68902.t1.exon1;Parent=MS.gene68902.t1
chr2.4 CDS 55018644 55019486 55018644 ID=cds.MS.gene68902.t1;Parent=MS.gene68902.t1
Gene Sequence

>MS.gene68902

ATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACATTGTCTCTACGAACCCTAGAGTTTTGTATGCAAGCCTCCAGAATCAAATAGTCCCAACCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGCAAAATTGAGTTACAATGCAAATTTACTTTGTGATCGTACTGTGTATAATAACATCACAATGCTGATTGAGAATGGGGTGTCTGACTCAAACATTAAAACATTGCTGAGGGAACAGACTCGGATATTCAAGAGACGTGACATGCTCAAGTCGGTGAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGCAGCATTGCATGCCAAAACAACAGTAATCAATACCCTATGGAAAGAGAAAGTTGATACCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTTGAAGCATTTAGAAAGAAACCTTATTGTATGTTAACATCCATTGAAAAAATAAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAACACCTTATATTTTGTCAGTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAATGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTCTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG
Protein sequence

>MS.gene68902.t1

MIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVSTNPRVLYASLQNQIVPTYELVYRFLQSDKEILAKLSYNANLLCDRTVYNNITMLIENGVSDSNIKTLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGAALHAKTTVINTLWKEKVDTFKKWGWSEEDSLEAFRKKPYCMLTSIEKINLVMNFWVNQLGWDAMAIAKTPYILSVSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEASYLLKLYEEKRNLG