AlfalfaGEDB | MS.gene68903

M. sativa cultivar XinJiangDaYe / MS.gene68903

KEGG: K15032
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02536.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68903.t1	KEH37235.1	77.6	152	8	1	1	126	228	379	6.80E-59	236.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68903.t1	A0A072VGE0	77.6	152	8	1	1	126	228	379	4.9e-59	236.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene68903.t1	TR	mTERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68903.t1	MTR_2g436460	77.632	152	8	1	1	126	228	379	9.18e-79	237
MS.gene68903.t1	MTR_2g437180	51.351	148	47	2	1	124	235	381	1.54e-42	144
MS.gene68903.t1	MTR_2g437200	51.007	149	46	2	1	123	251	398	2.20e-40	138
MS.gene68903.t1	MTR_2g437260	58.929	112	45	1	1	112	219	329	2.38e-40	138
MS.gene68903.t1	MTR_2g437040	48.026	152	52	2	1	126	223	373	1.40e-38	133
MS.gene68903.t1	MTR_2g437170	47.333	150	52	2	1	124	243	391	2.55e-38	133
MS.gene68903.t1	MTR_2g437150	46.053	152	55	2	1	126	243	393	9.07e-38	132
MS.gene68903.t1	MTR_2g437240	48.322	149	50	2	1	123	251	398	9.36e-37	129
MS.gene68903.t1	MTR_2g437130	45.395	152	56	2	1	126	224	374	1.17e-36	128
MS.gene68903.t1	MTR_2g437160	47.333	150	52	2	1	124	157	305	5.19e-36	125
MS.gene68903.t1	MTR_2g437160	47.333	150	52	2	1	124	248	396	5.40e-36	127
MS.gene68903.t1	MTR_2g437940	48.667	150	50	2	1	124	207	355	6.49e-36	126
MS.gene68903.t1	MTR_2g437080	49.587	121	60	1	1	121	223	342	7.50e-36	126
MS.gene68903.t1	MTR_2g076320	45.333	150	55	2	1	124	236	384	1.77e-35	125
MS.gene68903.t1	MTR_4g120380	52.679	112	52	1	1	112	225	335	2.35e-35	125
MS.gene68903.t1	MTR_4g120380	45.763	59	32	0	49	107	351	409	1.24e-12	63.5
MS.gene68903.t1	MTR_2g437090	45.802	131	63	2	1	124	11	140	4.65e-35	119
MS.gene68903.t1	MTR_4g120380	51.724	116	55	1	1	116	225	339	4.88e-35	124
MS.gene68903.t1	MTR_2g437990	46.000	150	54	2	1	124	235	383	9.49e-35	124
MS.gene68903.t1	MTR_2g436440	45.270	148	55	1	5	126	232	379	1.04e-34	123
MS.gene68903.t1	MTR_2g438020	46.667	150	53	2	1	124	290	438	1.37e-34	124
MS.gene68903.t1	MTR_2g437030	50.000	116	57	1	1	116	309	423	1.78e-34	124
MS.gene68903.t1	MTR_2g437020	51.724	116	55	1	1	116	224	338	1.86e-34	122
MS.gene68903.t1	MTR_2g437960	46.309	149	53	2	1	123	239	386	3.69e-34	122
MS.gene68903.t1	MTR_2g437060	47.934	121	62	1	1	121	238	357	3.87e-34	122
MS.gene68903.t1	MTR_2g437120	46.667	150	52	3	1	124	237	384	1.12e-33	121
MS.gene68903.t1	MTR_2g437960	46.309	149	53	2	1	123	239	386	1.50e-33	121
MS.gene68903.t1	MTR_2g438010	44.667	150	56	2	1	124	256	404	1.61e-33	121
MS.gene68903.t1	MTR_2g436400	49.138	116	58	1	1	116	230	344	4.21e-33	119
MS.gene68903.t1	MTR_4g119550	42.105	152	61	2	1	126	233	383	5.22e-33	119
MS.gene68903.t1	MTR_2g437100	45.638	149	54	2	1	123	248	395	5.62e-33	119
MS.gene68903.t1	MTR_2g019810	44.595	148	55	3	1	123	214	359	3.03e-30	111
MS.gene68903.t1	MTR_4g119580	48.598	107	54	1	1	107	225	330	3.42e-29	108
MS.gene68903.t1	MTR_2g019840	39.073	151	65	3	1	126	226	374	1.86e-22	90.9
MS.gene68903.t1	MTR_3g450500	41.509	106	61	1	1	106	225	329	5.25e-21	86.7
MS.gene68903.t1	MTR_4g007490	35.514	107	68	1	1	107	223	328	1.46e-15	71.6
MS.gene68903.t1	MTR_3g085240	45.333	75	31	1	51	125	176	240	1.51e-15	70.5
MS.gene68903.t1	MTR_4g119570	36.449	107	34	2	1	107	117	189	1.19e-13	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68903.t1	AT5G07900	37.778	135	69	4	1	124	254	384	4.98e-21	87.0
MS.gene68903.t1	AT1G21150	35.398	113	72	1	2	114	206	317	3.04e-16	73.6
MS.gene68903.t1	AT1G21150	35.398	113	72	1	2	114	306	417	4.18e-16	73.6
MS.gene68903.t1	AT1G21150	35.398	113	72	1	2	114	242	353	4.43e-16	73.2
MS.gene68903.t1	AT1G61990	30.986	142	62	2	1	107	226	366	3.54e-14	67.8
MS.gene68903.t1	AT1G61990	30.986	142	62	2	1	107	226	366	3.54e-14	67.8
MS.gene68903.t1	AT1G62010	28.235	170	74	4	1	123	217	385	6.52e-13	64.3
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT1G61970	29.577	142	64	2	1	107	233	373	9.56e-13	63.9
MS.gene68903.t1	AT5G64950	33.696	92	61	0	13	104	237	328	1.32e-12	63.5

Find 31 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATAAGGTGATGAGATTT+TGG	0.153456	2.4:+55015149	MS.gene68903:CDS
AAAAGTATGTGCAGTGTTTA+AGG	0.171840	2.4:+55015353	MS.gene68903:intron
TGATTTCGACATGGCCCTTA+TGG	0.304710	2.4:-55015017	None:intergenic
TTTCGTATAGTTTGGAGAAT+AGG	0.328948	2.4:+55015224	MS.gene68903:CDS
TTCGTATAGTTTGGAGAATA+GGG	0.359509	2.4:+55015225	MS.gene68903:CDS
AAGTTGTTGTCTTTAAGAAA+TGG	0.363837	2.4:+55015053	MS.gene68903:CDS
TCAAGCATTGTTAGCCATAA+GGG	0.366479	2.4:+55015003	MS.gene68903:CDS
ATCCACGGAGAAAATTAATA+AGG	0.367860	2.4:+55015135	MS.gene68903:CDS
TTGGGTCAATGAGTTGGGTT+GGG	0.371128	2.4:+55015168	MS.gene68903:CDS
AGTTGTTGTCTTTAAGAAAT+GGG	0.373623	2.4:+55015054	MS.gene68903:CDS
AAATGGTTCTTGAAGCGTTT+AGG	0.395855	2.4:+55015089	MS.gene68903:CDS
GTCAAGCATTGTTAGCCATA+AGG	0.402668	2.4:+55015002	MS.gene68903:CDS
TGAGTTGGGTTGGGACTCTT+CGG	0.410843	2.4:+55015177	MS.gene68903:CDS
ACTCTTCGGCTCTTGTCAAA+AGG	0.469912	2.4:+55015191	MS.gene68903:CDS
TTCTTGAAGCGTTTAGGAAG+CGG	0.487125	2.4:+55015095	MS.gene68903:CDS
TTTGGGTCAATGAGTTGGGT+TGG	0.489140	2.4:+55015167	MS.gene68903:CDS
ATGGGGTTGGTCTGATGAAA+TGG	0.499033	2.4:+55015072	MS.gene68903:CDS
GGCCATGTCGAAATCACGTT+GGG	0.500596	2.4:+55015024	MS.gene68903:CDS
GTTGTTGTCTTTAAGAAATG+GGG	0.513781	2.4:+55015055	MS.gene68903:CDS
TGCGAAACAACACAAGCCCT+TGG	0.528307	2.4:-55015253	None:intergenic
TTCGGCTCTTGTCAAAAGGC+CGG	0.528513	2.4:+55015195	MS.gene68903:CDS
TGGAGAATAGGGTCATTCCA+AGG	0.540810	2.4:+55015236	MS.gene68903:CDS
TTGTCTTTAAGAAATGGGGT+TGG	0.562623	2.4:+55015059	MS.gene68903:CDS
AGTGTGTATGTTAGCATCCA+CGG	0.577187	2.4:+55015120	MS.gene68903:CDS
GGAGAATAGGGTCATTCCAA+GGG	0.578298	2.4:+55015237	MS.gene68903:CDS
GGCTCTTGTCAAAAGGCCGG+AGG	0.586864	2.4:+55015198	MS.gene68903:CDS
GTTTCGCATTTGATCTCCAG+AGG	0.592319	2.4:+55015268	MS.gene68903:CDS
AGTATGTGCAGTGTTTAAGG+AGG	0.612400	2.4:+55015356	MS.gene68903:intron
GGGCCATGTCGAAATCACGT+TGG	0.657777	2.4:+55015023	MS.gene68903:CDS
ATCCCAACGTGATTTCGACA+TGG	0.666340	2.4:-55015026	None:intergenic
GTGGATGCTAACATACACAC+TGG	0.685589	2.4:-55015118	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGCTATACCTAGAAAAAA+TGG	+	chr2.4:55015395-55015414	MS.gene68903:CDS	25.0%
!	AAGCTATACCTAGAAAAAAT+GGG	+	chr2.4:55015396-55015415	MS.gene68903:CDS	25.0%
!!	AAGTTGTTGTCTTTAAGAAA+TGG	+	chr2.4:55015053-55015072	MS.gene68903:CDS	25.0%
!!	ACATTTTCATTAACACCAAA+TGG	-	chr2.4:55015325-55015344	None:intergenic	25.0%
!!	AGTTGTTGTCTTTAAGAAAT+GGG	+	chr2.4:55015054-55015073	MS.gene68903:CDS	25.0%
!!	TAATAAGGTGATGAGATTTT+GGG	+	chr2.4:55015150-55015169	MS.gene68903:CDS	25.0%
!!	TTAATAAGGTGATGAGATTT+TGG	+	chr2.4:55015149-55015168	MS.gene68903:CDS	25.0%
	AAACTATACGAAAAAACCTC+CGG	-	chr2.4:55015217-55015236	None:intergenic	30.0%
	ATCCACGGAGAAAATTAATA+AGG	+	chr2.4:55015135-55015154	MS.gene68903:CDS	30.0%
	ATTGAATTGTCTACTCCATT+TGG	+	chr2.4:55015307-55015326	MS.gene68903:intron	30.0%
	TGACAAAAATTCACATTGGT+AGG	-	chr2.4:55014986-55015005	None:intergenic	30.0%
	TGCTTGACAAAAATTCACAT+TGG	-	chr2.4:55014990-55015009	None:intergenic	30.0%
	TTCGTATAGTTTGGAGAATA+GGG	+	chr2.4:55015225-55015244	MS.gene68903:CDS	30.0%
	TTTCGTATAGTTTGGAGAAT+AGG	+	chr2.4:55015224-55015243	MS.gene68903:CDS	30.0%
!	AAAAGTATGTGCAGTGTTTA+AGG	+	chr2.4:55015353-55015372	MS.gene68903:intron	30.0%
!	ATTCTTTTCTATCAAACCTC+TGG	-	chr2.4:55015287-55015306	None:intergenic	30.0%
!	GTTGTTGTCTTTAAGAAATG+GGG	+	chr2.4:55015055-55015074	MS.gene68903:CDS	30.0%
!	AAATGGTTCTTGAAGCGTTT+AGG	+	chr2.4:55015089-55015108	MS.gene68903:CDS	35.0%
!	CACCTTATTAATTTTCTCCG+TGG	-	chr2.4:55015140-55015159	None:intergenic	35.0%
!	TCAAGCATTGTTAGCCATAA+GGG	+	chr2.4:55015003-55015022	MS.gene68903:CDS	35.0%
!	TTGTCTTTAAGAAATGGGGT+TGG	+	chr2.4:55015059-55015078	MS.gene68903:CDS	35.0%
!!	AGATTTTGGGTCAATGAGTT+GGG	+	chr2.4:55015163-55015182	MS.gene68903:CDS	35.0%
!!!	GGAGGTTTTTTCGTATAGTT+TGG	+	chr2.4:55015216-55015235	MS.gene68903:CDS	35.0%
	AGTATGTGCAGTGTTTAAGG+AGG	+	chr2.4:55015356-55015375	MS.gene68903:intron	40.0%
	AGTGTGTATGTTAGCATCCA+CGG	+	chr2.4:55015120-55015139	MS.gene68903:CDS	40.0%
!	GTCAAGCATTGTTAGCCATA+AGG	+	chr2.4:55015002-55015021	MS.gene68903:CDS	40.0%
!	TTCTTGAAGCGTTTAGGAAG+CGG	+	chr2.4:55015095-55015114	MS.gene68903:CDS	40.0%
!!	GAGATTTTGGGTCAATGAGT+TGG	+	chr2.4:55015162-55015181	MS.gene68903:CDS	40.0%
	ACTCTTCGGCTCTTGTCAAA+AGG	+	chr2.4:55015191-55015210	MS.gene68903:CDS	45.0%
	ATCCCAACGTGATTTCGACA+TGG	-	chr2.4:55015029-55015048	None:intergenic	45.0%
	GGAGAATAGGGTCATTCCAA+GGG	+	chr2.4:55015237-55015256	MS.gene68903:CDS	45.0%
	GTGGATGCTAACATACACAC+TGG	-	chr2.4:55015121-55015140	None:intergenic	45.0%
	GTTTCGCATTTGATCTCCAG+AGG	+	chr2.4:55015268-55015287	MS.gene68903:CDS	45.0%
	TGATTTCGACATGGCCCTTA+TGG	-	chr2.4:55015020-55015039	None:intergenic	45.0%
	TGGAGAATAGGGTCATTCCA+AGG	+	chr2.4:55015236-55015255	MS.gene68903:CDS	45.0%
!	TTGGGTCAATGAGTTGGGTT+GGG	+	chr2.4:55015168-55015187	MS.gene68903:CDS	45.0%
!	TTTGGGTCAATGAGTTGGGT+TGG	+	chr2.4:55015167-55015186	MS.gene68903:CDS	45.0%
!!	ATGGGGTTGGTCTGATGAAA+TGG	+	chr2.4:55015072-55015091	MS.gene68903:CDS	45.0%
	GGCCATGTCGAAATCACGTT+GGG	+	chr2.4:55015024-55015043	MS.gene68903:CDS	50.0%
	TGCGAAACAACACAAGCCCT+TGG	-	chr2.4:55015256-55015275	None:intergenic	50.0%
	TTCGGCTCTTGTCAAAAGGC+CGG	+	chr2.4:55015195-55015214	MS.gene68903:CDS	50.0%
!!	TGAGTTGGGTTGGGACTCTT+CGG	+	chr2.4:55015177-55015196	MS.gene68903:CDS	50.0%
	GGGCCATGTCGAAATCACGT+TGG	+	chr2.4:55015023-55015042	MS.gene68903:CDS	55.0%
	GGCTCTTGTCAAAAGGCCGG+AGG	+	chr2.4:55015198-55015217	MS.gene68903:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	55014968	55015425	55014968	ID=MS.gene68903
chr2.4	mRNA	55014968	55015425	55014968	ID=MS.gene68903.t1;Parent=MS.gene68903
chr2.4	exon	55014968	55015289	55014968	ID=MS.gene68903.t1.exon1;Parent=MS.gene68903.t1
chr2.4	CDS	55014968	55015289	55014968	ID=cds.MS.gene68903.t1;Parent=MS.gene68903.t1
chr2.4	exon	55015367	55015425	55015367	ID=MS.gene68903.t1.exon2;Parent=MS.gene68903.t1
chr2.4	CDS	55015367	55015425	55015367	ID=cds.MS.gene68903.t1;Parent=MS.gene68903.t1

Gene Sequence

>MS.gene68903

ATGGGTTTTGATGATCCTACCAATGTGAATTTTTGTCAAGCATTGTTAGCCATAAGGGCCATGTCGAAATCACGTTGGGATGCGAAAGTTGTTGTCTTTAAGAAATGGGGTTGGTCTGATGAAATGGTTCTTGAAGCGTTTAGGAAGCGGCCAGTGTGTATGTTAGCATCCACGGAGAAAATTAATAAGGTGATGAGATTTTGGGTCAATGAGTTGGGTTGGGACTCTTCGGCTCTTGTCAAAAGGCCGGAGGTTTTTTCGTATAGTTTGGAGAATAGGGTCATTCCAAGGGCTTGTGTTGTTTCGCATTTGATCTCCAGAGGTTTGATAGAAAAGAATATTGAATTGTCTACTCCATTTGGTGTTAATGAAAATGTGTTTCTTGAAAAGTATGTGCAGTGTTTAAGGAGGAAAGAGATGATTTGTTAAAGCTATACCTAGAAAAAATGGGTGTCTGA

Protein sequence

>MS.gene68903.t1

MGFDDPTNVNFCQALLAIRAMSKSRWDAKVVVFKKWGWSDEMVLEAFRKRPVCMLASTEKINKVMRFWVNELGWDSSALVKRPEVFSYSLENRVIPRACVVSHLISRVFKEERDDLLKLYLEKMGV