AlfalfaGEDB | MS.gene69265

M. sativa cultivar XinJiangDaYe / MS.gene69265

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69265.t1	XP_003600845.2	80.3	314	38	2	1	299	1	305	8.00E-135	490

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69265.t1	Q9FLJ2	50.5	297	123	7	13	299	10	292	1.1e-63	245.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69265.t1	G7JAN2	80.3	314	38	2	1	299	1	305	5.8e-135	490.0

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene69265.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69265.t1	MTR_3g070030	80.255	314	38	2	1	299	1	305	0.0	512
MS.gene69265.t1	MTR_3g070040	77.926	299	38	4	12	294	1	287	6.65e-166	464
MS.gene69265.t1	MTR_7g011130	74.757	309	50	2	1	281	1	309	3.36e-164	460
MS.gene69265.t1	MTR_7g011120	74.368	277	54	4	12	281	1	267	3.58e-144	408
MS.gene69265.t1	MTR_6g012670	52.817	284	118	6	1	271	2	282	4.88e-90	271
MS.gene69265.t1	MTR_4g108760	51.333	300	125	9	13	297	11	304	1.88e-87	266
MS.gene69265.t1	MTR_3g435150	50.000	286	119	5	5	271	2	282	3.56e-81	248
MS.gene69265.t1	MTR_2g078700	70.886	158	43	2	19	174	23	179	4.05e-77	240
MS.gene69265.t1	MTR_6g032770	62.130	169	61	2	14	180	13	180	7.87e-73	229
MS.gene69265.t1	MTR_7g085260	62.500	176	61	3	5	176	3	177	1.00e-72	229

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69265.t1	AT5G61430	50.342	292	131	6	13	299	10	292	3.52e-86	261
MS.gene69265.t1	AT5G07680	50.000	280	125	4	10	286	8	275	3.22e-84	256
MS.gene69265.t1	AT5G07680	50.549	273	120	4	17	286	1	261	5.42e-83	253
MS.gene69265.t1	AT3G29035	48.110	291	122	6	3	287	8	275	2.35e-79	244
MS.gene69265.t1	AT5G39610	68.293	164	51	1	13	176	14	176	1.72e-76	235
MS.gene69265.t1	AT5G53950	72.078	154	40	2	19	170	17	169	1.20e-75	236
MS.gene69265.t1	AT5G18270	67.296	159	50	2	15	172	17	174	1.52e-73	229
MS.gene69265.t1	AT3G04060	65.455	165	55	2	7	170	8	171	1.88e-73	229
MS.gene69265.t1	AT5G18270	67.296	159	50	2	15	172	17	174	2.43e-73	229
MS.gene69265.t1	AT3G18400	64.557	158	52	1	19	176	5	158	1.08e-72	226

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Find 60 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGATGGTTCAAAACA+TGG	0.533594	3.2:-63780646	MS.gene69265:CDS
AAATCATTACCTTGTCAAAA+AGG	0.228485	3.2:-63782414	MS.gene69265:CDS
AAATGAAAAGGTCGAATTTC+CGG	0.216715	3.2:-63782474	MS.gene69265:CDS
AATGAAAAGGTCGAATTTCC+GGG	0.351715	3.2:-63782473	MS.gene69265:CDS
AATTCCCGACCATTGAGGTT+TGG	0.446812	3.2:+63780392	None:intergenic
AATTGGGATGAAGAAAACAT+TGG	0.537739	3.2:-63781280	MS.gene69265:CDS
AATTGTGGAAAGAAAAGGGA+TGG	0.607650	3.2:-63780658	MS.gene69265:CDS
AATTTACCTGGCAAATCCCA+TGG	0.582769	3.2:+63782332	None:intergenic
ACCAATAGAGCTACTAATGC+TGG	0.453301	3.2:-63781354	MS.gene69265:CDS
AGAATGTGTTACCCTCCAAT+CGG	0.588761	3.2:+63781199	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGAAAAGGTCGAATTTC+CGG	-	chr3.2:63780332-63780351	MS.gene69265:CDS	30.0%
AATTGGGATGAAGAAAACAT+TGG	-	chr3.2:63781526-63781545	MS.gene69265:intron	30.0%
ACACATGAGTATAGTCATAT+TGG	+	chr3.2:63780724-63780743	None:intergenic	30.0%
ACAGATGAATTTGCGAAAAA+AGG	-	chr3.2:63780679-63780698	MS.gene69265:CDS	30.0%
AGCATAATTGTGGAAAGAAA+AGG	-	chr3.2:63782143-63782162	MS.gene69265:intron	30.0%
ATAAATCAGACATAGTGGTT+TGG	+	chr3.2:63782231-63782250	None:intergenic	30.0%
ATAACAATGTCCTACTAAGA+CGG	-	chr3.2:63780895-63780914	MS.gene69265:intron	30.0%
ATAGATCGTCAATGAATGTT+TGG	+	chr3.2:63781857-63781876	None:intergenic	30.0%
ATATGAATAAGTGTGAACCA+TGG	-	chr3.2:63780457-63780476	MS.gene69265:CDS	30.0%
ATGAGATAAATCAGACATAG+TGG	+	chr3.2:63782236-63782255	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	63780294	63782534	63780294	ID=MS.gene69265
chr3.2	mRNA	63780294	63782534	63780294	ID=MS.gene69265.t1;Parent=MS.gene69265
chr3.2	exon	63782339	63782534	63782339	ID=MS.gene69265.t1.exon1;Parent=MS.gene69265.t1
chr3.2	CDS	63782339	63782534	63782339	ID=cds.MS.gene69265.t1;Parent=MS.gene69265.t1
chr3.2	exon	63781170	63781450	63781170	ID=MS.gene69265.t1.exon2;Parent=MS.gene69265.t1
chr3.2	CDS	63781170	63781450	63781170	ID=cds.MS.gene69265.t1;Parent=MS.gene69265.t1
chr3.2	exon	63780294	63780716	63780294	ID=MS.gene69265.t1.exon3;Parent=MS.gene69265.t1
chr3.2	CDS	63780294	63780716	63780294	ID=cds.MS.gene69265.t1;Parent=MS.gene69265.t1

Gene Sequence

>MS.gene69265

ATGGGATCAAATGAGAATGTTTCCAATCAAAAAATGGAAAATGAAAAGGTCGAATTTCCGGGTTGTTTTCGATTCTATCCGACCGATGAAGAGCTTATAAATCATTACCTTGTCAAAAAGGTTGATGACAATAGCTTCTCTGCTATAGCTATTGCTGAAGTTGATATGAATAAGTGTGAACCATGGGATTTGCCAGGTAAATTTATTGTTTTTTTATAAGTAAATACTAGTTGTTATTATATTATATTAGTATGTCTAACCAAAAAAAAAAATAGTAATATGGATATGAAAAACTATGCGTTGAAGTATTTATTTTTTTTTGAAAAATAAACGATGTTGATAGAGTGCATCGATAACACATACATTTAAAGTCATTAAAAACCAAACAGATGAATTTGCGAAAAAAGGAGCAAAATACACAAGACTACCAATATGACTATACTCATGTGTCAAAAATATTGATGCCTACATATCTGTAACATTGATGACACCAAAGTCATTGATTAGATCTGGACTAGATCAAAGCTAATCTTTCAACCTGAGTCAAATAAACACTGCATTAAGATGAAAAATCAAGAAAACACTACAACAACGAGAAATCATAACAATGTCCTACTAAGACGGTGAAATCATAAAAACGTACCAAAGAAGACTTAATTTATGTGGAAATCACTTATTTAACTAAAAAAAGAAAGAAAAACAATTTGGAATGGTGATTACGGGACAAAATTAACCCTAAAACATCCTTCATTGGTAGATCTGGATGAAAAAGAAGAAGAAAAATGGTGACCACTAGGGTTTGGCAAGGAGAAAAGCGAGTGGTTTAATTACGCCTTAGAAATATTGTCGTTATCATCTTACTTTGATGGTTATATATTTTCTCTTAATTACTATGTATCACCGACACATCATAATGTGTAAGGTATGCACACCAACATGACACTATCAGATGTGGTTAAATTTATTATTTTATTTTTTAAAGTTATTACGAATGTGGATTTGTCAGTGTCATGTCTAATGTATGTACTATGTCTTTCGGTGTTTCATAACTTAATTATGCTTTCTTACTTTTTGGTTTATTCAGAGATGGCTAAAATGGGAGAAGCAGAATGGTATTTTTTCTATGTGAGGGAGAGAAAATACCCAACTGGTCAAAGGACCAATAGAGCTACTAATGCTGGATATTGGAAAGCAACAGGCAAAGACAAAGAGATTTTCAAGGGAAATGCATTAATTGGGATGAAGAAAACATTGGTTTTCTACATAGGAAGAGCTCCGAGTGGTGAAAAGACCAATTGGGTCATGCATGAATACCGATTGGAGGGTAACACATTCTCTGAGCACAACCTATCCACACATGGAATGGTAATATTGCTATAATTTTTTTATTTGTTTTTGTCTATGATTTCTACTCTTACTACATGTGAAAATTTGTTAGTGTATGTTCACATTGACCATGTGTTTAATAGAATTACCTTATGTTACCGTAATTTTGGATAAAACTTTACTTTTGAATTTTAACAAAATTATTGTGTCATTATTGTCAAACTTATCGTCGATCCAAACATTCATTGACGATCTATTTGTAATAAATAAAGTAAACTATTGTATGTTTGATTACCATCATAAAAATAAGTTTTATAAAAAATCAATTAAAAAACCTAACTTTTAGAGAAACTACTGGAAACAACTTTTTGTTTGAATTCTTTCAAATACTAGTATTCTAAAAAATATGTAGAAATGAATAAAAAGAAGAAGATTGTTTTGTTAAAAATAATGTTTTTTCATCTAATATTAATGTGTTAACCTTTTTTTTAGAGTGAATGGGTTCTCAGTAGAGTCTTTGAGAAGCATAATTGTGGAAAGAAAAGGGATGGTTCAAAACATGGAAGGTTAAACACCTCTAGAGAAGGACCATCAAATACTAATGAAAACCAAACCACTATGTCTGATTTATCTCATGTGTTCAATTCATTCTCCAATCCAAATCAAACAGAGAAACAAAAAACACAAAATGACAACATTGTTCATAACAATGAAACTAGTATCTTGAACATTTCATCCTCTTCAAAACAAATGGATGTGTATCCTTTAGCTGAAGTAATAACTGCTGGCTTGAACCAAACCTCAATGGTCGGGAATTCGTCAAACTTTTTCTTCTCTCAAGAACAATCACTGTTGAGGATGCAGTTGGAGAATGAGAATCTTGGAACAACCTCAAGACAAAGCCTAAACCAATAG

Protein sequence

>MS.gene69265.t1

MGSNENVSNQKMENEKVEFPGCFRFYPTDEELINHYLVKKVDDNSFSAIAIAEVDMNKCEPWDLPEMAKMGEAEWYFFYVRERKYPTGQRTNRATNAGYWKATGKDKEIFKGNALIGMKKTLVFYIGRAPSGEKTNWVMHEYRLEGNTFSEHNLSTHGMSEWVLSRVFEKHNCGKKRDGSKHGRLNTSREGPSNTNENQTTMSDLSHVFNSFSNPNQTEKQKTQNDNIVHNNETSILNISSSSKQMDVYPLAEVITAGLNQTSMVGNSSNFFFSQEQSLLRMQLENENLGTTSRQSLNQ