AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69436


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69436.t1 MTR_3g113070 88.235 238 13 5 1 237 1 224 5.68e-149 414
MS.gene69436.t1 MTR_1g023260 47.670 279 82 12 1 233 1 261 1.34e-65 204
MS.gene69436.t1 MTR_1g023260 56.354 181 45 5 1 150 1 178 8.10e-61 189
MS.gene69436.t1 MTR_3g117320 48.039 204 57 5 1 159 1 200 2.31e-56 179
MS.gene69436.t1 MTR_4g071200 41.176 204 83 4 1 170 1 201 1.11e-49 162
MS.gene69436.t1 MTR_2g073370 53.271 107 47 1 1 107 1 104 6.01e-35 126
MS.gene69436.t1 MTR_2g099010 33.621 232 124 5 1 216 1 218 6.19e-35 125
MS.gene69436.t1 MTR_2g099010 34.483 232 121 5 1 216 1 217 4.59e-34 122
MS.gene69436.t1 MTR_4g067320 51.402 107 49 1 1 107 1 104 1.75e-31 115
MS.gene69436.t1 MTR_4g067320 51.402 107 49 1 1 107 1 104 1.89e-31 115
MS.gene69436.t1 MTR_1g023260 39.149 235 74 13 45 233 42 253 4.05e-28 107
MS.gene69436.t1 MTR_5g021580 40.351 114 62 2 1 114 1 108 9.75e-22 89.0
MS.gene69436.t1 MTR_5g021580 40.351 114 62 2 1 114 1 108 1.32e-21 89.0
MS.gene69436.t1 MTR_1g013450 33.113 151 84 2 5 150 6 144 9.48e-16 75.5
MS.gene69436.t1 MTR_3g105710 37.895 95 47 1 5 99 6 88 2.75e-13 68.2
MS.gene69436.t1 MTR_4g128930 35.789 95 49 1 5 99 22 104 1.12e-12 67.0
MS.gene69436.t1 MTR_7g018170 30.000 120 72 1 5 124 26 133 1.62e-12 66.6
MS.gene69436.t1 MTR_5g069480 30.303 99 57 1 1 99 1 87 3.55e-11 62.0
MS.gene69436.t1 MTR_5g069480 30.303 99 57 1 1 99 1 87 6.00e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69436.t1 AT1G75540 38.272 324 108 9 1 236 1 320 3.41e-58 187
MS.gene69436.t1 AT4G39070 43.478 207 57 3 1 149 1 205 5.10e-47 156
MS.gene69436.t1 AT1G78600 52.336 107 48 1 1 107 1 104 7.41e-35 126
MS.gene69436.t1 AT1G06040 53.271 107 47 1 1 107 1 104 1.05e-34 122
MS.gene69436.t1 AT1G78600 52.336 107 48 1 1 107 1 104 1.42e-34 126
MS.gene69436.t1 AT2G31380 53.271 107 47 1 1 107 1 104 5.11e-34 122
MS.gene69436.t1 AT1G06040 53.271 107 47 1 1 107 1 104 1.02e-33 122
MS.gene69436.t1 AT4G10240 41.912 136 70 3 1 133 1 130 1.08e-29 109
MS.gene69436.t1 AT4G38960 42.105 114 60 2 1 114 1 108 3.15e-23 93.2
MS.gene69436.t1 AT4G38960 42.105 114 60 2 1 114 1 108 3.15e-23 93.2
MS.gene69436.t1 AT2G21320 42.105 114 60 2 1 114 1 108 6.17e-23 92.0
MS.gene69436.t1 AT2G24790 38.947 95 46 1 5 99 8 90 2.04e-15 73.2
MS.gene69436.t1 AT4G38960 30.573 157 60 3 1 114 1 151 2.78e-15 72.8
MS.gene69436.t1 AT2G24790 38.947 95 46 1 5 99 8 90 6.93e-15 72.8
MS.gene69436.t1 AT5G15850 34.862 109 57 2 5 112 12 107 6.33e-14 70.5
MS.gene69436.t1 AT3G02380 32.743 113 62 2 5 116 16 115 7.90e-14 70.1
MS.gene69436.t1 AT5G24930 36.842 95 48 1 5 99 6 88 1.16e-13 69.7
MS.gene69436.t1 AT5G15840 29.839 124 75 1 5 128 20 131 9.31e-13 66.6
MS.gene69436.t1 AT5G15840 29.839 124 75 1 5 128 20 131 1.72e-12 66.2
MS.gene69436.t1 AT1G28050 38.462 104 52 3 2 105 6 97 1.91e-12 66.6
MS.gene69436.t1 AT2G33500 34.951 103 55 1 3 105 10 100 3.94e-12 65.5
MS.gene69436.t1 AT2G33500 34.951 103 55 1 3 105 10 100 3.98e-12 65.5

Find 64 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGATGTGGCAATGTTAAT+TGG 0.169105 3.3:-92894578 None:intergenic
AAATGGTTGGGAGAATTATT+AGG 0.172908 3.3:-92893853 None:intergenic
AAGAAACCTATTATGCTTCT+TGG 0.240931 3.3:-92894152 None:intergenic
ACCAATTTCAGCAGCAGTTT+GGG 0.246710 3.3:+92894523 MS.gene69436:CDS
CCCCAAACTGCTGCTGAAAT+TGG 0.248979 3.3:-92894524 None:intergenic
CACCAATTTCAGCAGCAGTT+TGG 0.303898 3.3:+92894522 MS.gene69436:CDS
TATTCATGAGGAAAACATTC+TGG 0.307233 3.3:+92894399 MS.gene69436:CDS
AACACACCAAGAAGCATAAT+AGG 0.390006 3.3:+92894146 MS.gene69436:CDS
CATAATAGGTTTCTTCTAAG+TGG 0.399657 3.3:+92894160 MS.gene69436:CDS
GAGAGAGAAGCGCCGGTGTT+TGG 0.406481 3.3:-92893824 None:intergenic
AAGATGTGGCAATGTTAATT+GGG 0.407815 3.3:-92894577 None:intergenic
TCAGAGAGAGGGAAATGGTT+GGG 0.423810 3.3:-92893865 None:intergenic
GAGTGTGATTTGAAGATTCA+TGG 0.427331 3.3:+92894115 MS.gene69436:CDS
CTACAACAGCAGAATCATAT+TGG 0.434122 3.3:+92894331 MS.gene69436:CDS
AATGGTTGGGAGAATTATTA+GGG 0.446787 3.3:-92893852 None:intergenic
TCATGAGAGGGAACAGAAGA+AGG 0.454520 3.3:-92894307 None:intergenic
GTGGTCGCAGGTGGAGCATA+AGG 0.456556 3.3:-92893773 None:intergenic
ACAGTTGGAGGAGGAGAAGC+AGG 0.467510 3.3:-92894199 None:intergenic
ATAATAGGTTTCTTCTAAGT+GGG 0.482873 3.3:+92894161 MS.gene69436:CDS
TTCTCTAGCGAGAGCATGTG+TGG 0.496184 3.3:+92894427 MS.gene69436:CDS
GGAGTTTCTTCATGAACAGT+TGG 0.498814 3.3:-92894214 None:intergenic
AGAGAAGCGCCGGTGTTTGG+AGG 0.498896 3.3:-92893821 None:intergenic
GAACAGAAGAAGGGGAGTCA+AGG 0.507606 3.3:-92894297 None:intergenic
ATCAGAGAGAGGGAAATGGT+TGG 0.514446 3.3:-92893866 None:intergenic
ATGGTGGACACGGTGGTCGC+AGG 0.523492 3.3:-92893785 None:intergenic
CAAACTGCTGCTGAAATTGG+TGG 0.525028 3.3:-92894521 None:intergenic
AAATATCAGAGAGAGGGAAA+TGG 0.531067 3.3:-92893870 None:intergenic
GTGGACACGGTGGTCGCAGG+TGG 0.531543 3.3:-92893782 None:intergenic
CAGCAGAATCATATTGGTAA+AGG 0.531622 3.3:+92894337 MS.gene69436:CDS
AAATCTTCAAACTTCCAACC+TGG 0.533797 3.3:-92894274 None:intergenic
GGTTGGGAGAATTATTAGGG+TGG 0.538692 3.3:-92893849 None:intergenic
TGGAGGCGAGTTTGTTAGCA+TGG 0.539793 3.3:-92893804 None:intergenic
GTTTCTTCATGAACAGTTGG+AGG 0.542162 3.3:-92894211 None:intergenic
TGTGGCAATGTTAATTGGGA+GGG 0.543395 3.3:-92894573 None:intergenic
CATGAGAGGGAACAGAAGAA+GGG 0.544618 3.3:-92894306 None:intergenic
TCTTCATGAACAGTTGGAGG+AGG 0.551533 3.3:-92894208 None:intergenic
TGCTGTTGTAGTTCATGAGA+GGG 0.552947 3.3:-92894319 None:intergenic
GTTTGTTAGCATGGTGGACA+CGG 0.562165 3.3:-92893795 None:intergenic
CTGCTGTTGTAGTTCATGAG+AGG 0.569968 3.3:-92894320 None:intergenic
GCATAAGGCGGCTTCATCCG+CGG 0.570214 3.3:-92893758 None:intergenic
CTATGTTTGTAGGAAAGAAG+AGG 0.575915 3.3:+92894052 MS.gene69436:intron
AGTGCGACATCTGTAACAAA+CGG 0.576581 3.3:+92893716 MS.gene69436:CDS
TACTTGATAAACACTATCCC+AGG 0.579028 3.3:+92894256 MS.gene69436:CDS
CCAATTTCAGCAGCAGTTTG+GGG 0.580053 3.3:+92894524 MS.gene69436:CDS
AATCTTCAAACTTCCAACCT+GGG 0.582408 3.3:-92894273 None:intergenic
GAATAATACAGTGGTGCTTG+AGG 0.582637 3.3:-92894460 None:intergenic
TTGGGAGGGATTGTTATCTG+TGG 0.583370 3.3:-92894559 None:intergenic
ATGAGAGGGAACAGAAGAAG+GGG 0.584490 3.3:-92894305 None:intergenic
CGACATCTGTAACAAACGGC+AGG 0.587499 3.3:+92893720 MS.gene69436:CDS
TACTCAGATATAGTAAAACT+AGG 0.594122 3.3:-92894235 None:intergenic
GTTTGTTACAGATGTCGCAC+TGG 0.600679 3.3:-92893714 None:intergenic
TCTCTAGCGAGAGCATGTGT+GGG 0.602430 3.3:+92894428 MS.gene69436:CDS
ATGTGGCAATGTTAATTGGG+AGG 0.602797 3.3:-92894574 None:intergenic
GGTGGTTGAGAGAGAAGCGC+CGG 0.603996 3.3:-92893831 None:intergenic
CGAGGCAAATATCAGAGAGA+GGG 0.604337 3.3:-92893876 None:intergenic
GTCGCAGGTGGAGCATAAGG+CGG 0.608572 3.3:-92893770 None:intergenic
ACGAGGCAAATATCAGAGAG+AGG 0.619991 3.3:-92893877 None:intergenic
CAATTTCAGCAGCAGTTTGG+GGG 0.628510 3.3:+92894525 MS.gene69436:CDS
TGATAAACACTATCCCAGGT+TGG 0.630031 3.3:+92894260 MS.gene69436:CDS
ACAAACTCGCCTCCAAACAC+CGG 0.644751 3.3:+92893812 MS.gene69436:CDS
TGTTAATTATCATATTCATG+AGG 0.653612 3.3:+92894387 MS.gene69436:CDS
AGGCGAGTTTGTTAGCATGG+TGG 0.655308 3.3:-92893801 None:intergenic
TGTTAGCATGGTGGACACGG+TGG 0.691209 3.3:-92893792 None:intergenic
GGCTTCTTTATTCTGCACCG+CGG 0.708284 3.3:+92893741 MS.gene69436:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAAAAGAATGAATAT+TGG - chr3.3:92893911-92893930 None:intergenic 15.0%
!! TGTTAATTATCATATTCATG+AGG + chr3.3:92894387-92894406 MS.gene69436:CDS 20.0%
!!! ACTGTATTATTCTGAAAAAA+TGG + chr3.3:92894471-92894490 MS.gene69436:CDS 20.0%
!!! ATTTTTTCAGAATAATACAG+TGG - chr3.3:92894472-92894491 None:intergenic 20.0%
! ATAATAGGTTTCTTCTAAGT+GGG + chr3.3:92894161-92894180 MS.gene69436:CDS 25.0%
! TACTCAGATATAGTAAAACT+AGG - chr3.3:92894238-92894257 None:intergenic 25.0%
AAATGGTTGGGAGAATTATT+AGG - chr3.3:92893856-92893875 None:intergenic 30.0%
AAGAAACCTATTATGCTTCT+TGG - chr3.3:92894155-92894174 None:intergenic 30.0%
AATGGTTGGGAGAATTATTA+GGG - chr3.3:92893855-92893874 None:intergenic 30.0%
CATAATAGGTTTCTTCTAAG+TGG + chr3.3:92894160-92894179 MS.gene69436:CDS 30.0%
TATTCATGAGGAAAACATTC+TGG + chr3.3:92894399-92894418 MS.gene69436:CDS 30.0%
!! GAGTTTGTTTCTATGTTTGT+AGG + chr3.3:92894042-92894061 MS.gene69436:intron 30.0%
!!! AAGAGGTTTTGTTTTTTGTC+AGG + chr3.3:92894069-92894088 MS.gene69436:CDS 30.0%
AAATATCAGAGAGAGGGAAA+TGG - chr3.3:92893873-92893892 None:intergenic 35.0%
AAATCTTCAAACTTCCAACC+TGG - chr3.3:92894277-92894296 None:intergenic 35.0%
AATCTTCAAACTTCCAACCT+GGG - chr3.3:92894276-92894295 None:intergenic 35.0%
CAGCAGAATCATATTGGTAA+AGG + chr3.3:92894337-92894356 MS.gene69436:CDS 35.0%
CTACAACAGCAGAATCATAT+TGG + chr3.3:92894331-92894350 MS.gene69436:CDS 35.0%
CTATGTTTGTAGGAAAGAAG+AGG + chr3.3:92894052-92894071 MS.gene69436:intron 35.0%
GAATATTGGAGAGAATTACG+AGG - chr3.3:92893897-92893916 None:intergenic 35.0%
GAGTGTGATTTGAAGATTCA+TGG + chr3.3:92894115-92894134 MS.gene69436:CDS 35.0%
TACTTGATAAACACTATCCC+AGG + chr3.3:92894256-92894275 MS.gene69436:CDS 35.0%
! AACACACCAAGAAGCATAAT+AGG + chr3.3:92894146-92894165 MS.gene69436:CDS 35.0%
ACCAATTTCAGCAGCAGTTT+GGG + chr3.3:92894523-92894542 MS.gene69436:CDS 40.0%
AGTGCGACATCTGTAACAAA+CGG + chr3.3:92893716-92893735 MS.gene69436:CDS 40.0%
GAATAATACAGTGGTGCTTG+AGG - chr3.3:92894463-92894482 None:intergenic 40.0%
GGAGTTTCTTCATGAACAGT+TGG - chr3.3:92894217-92894236 None:intergenic 40.0%
GTTTCTTCATGAACAGTTGG+AGG - chr3.3:92894214-92894233 None:intergenic 40.0%
TGATAAACACTATCCCAGGT+TGG + chr3.3:92894260-92894279 MS.gene69436:CDS 40.0%
TGCTGTTGTAGTTCATGAGA+GGG - chr3.3:92894322-92894341 None:intergenic 40.0%
ACGAGGCAAATATCAGAGAG+AGG - chr3.3:92893880-92893899 None:intergenic 45.0%
ATCAGAGAGAGGGAAATGGT+TGG - chr3.3:92893869-92893888 None:intergenic 45.0%
ATGAGAGGGAACAGAAGAAG+GGG - chr3.3:92894308-92894327 None:intergenic 45.0%
CAAACTGCTGCTGAAATTGG+TGG - chr3.3:92894524-92894543 None:intergenic 45.0%
CAATTTCAGCAGCAGTTTGG+GGG + chr3.3:92894525-92894544 MS.gene69436:CDS 45.0%
CACCAATTTCAGCAGCAGTT+TGG + chr3.3:92894522-92894541 MS.gene69436:CDS 45.0%
CATGAGAGGGAACAGAAGAA+GGG - chr3.3:92894309-92894328 None:intergenic 45.0%
CCAATTTCAGCAGCAGTTTG+GGG + chr3.3:92894524-92894543 MS.gene69436:CDS 45.0%
CGAGGCAAATATCAGAGAGA+GGG - chr3.3:92893879-92893898 None:intergenic 45.0%
CTGCTGTTGTAGTTCATGAG+AGG - chr3.3:92894323-92894342 None:intergenic 45.0%
GGTTGGGAGAATTATTAGGG+TGG - chr3.3:92893852-92893871 None:intergenic 45.0%
TCAGAGAGAGGGAAATGGTT+GGG - chr3.3:92893868-92893887 None:intergenic 45.0%
TCATGAGAGGGAACAGAAGA+AGG - chr3.3:92894310-92894329 None:intergenic 45.0%
TCTTCATGAACAGTTGGAGG+AGG - chr3.3:92894211-92894230 None:intergenic 45.0%
TTGGGAGGGATTGTTATCTG+TGG - chr3.3:92894562-92894581 None:intergenic 45.0%
! GTTTGTTACAGATGTCGCAC+TGG - chr3.3:92893717-92893736 None:intergenic 45.0%
!! GTTTGTTAGCATGGTGGACA+CGG - chr3.3:92893798-92893817 None:intergenic 45.0%
ACAAACTCGCCTCCAAACAC+CGG + chr3.3:92893812-92893831 MS.gene69436:CDS 50.0%
CCCCAAACTGCTGCTGAAAT+TGG - chr3.3:92894527-92894546 None:intergenic 50.0%
CGACATCTGTAACAAACGGC+AGG + chr3.3:92893720-92893739 MS.gene69436:CDS 50.0%
GAACAGAAGAAGGGGAGTCA+AGG - chr3.3:92894300-92894319 None:intergenic 50.0%
GGCTTCTTTATTCTGCACCG+CGG + chr3.3:92893741-92893760 MS.gene69436:CDS 50.0%
! TCTCTAGCGAGAGCATGTGT+GGG + chr3.3:92894428-92894447 MS.gene69436:CDS 50.0%
! TTCTCTAGCGAGAGCATGTG+TGG + chr3.3:92894427-92894446 MS.gene69436:CDS 50.0%
!! AGGCGAGTTTGTTAGCATGG+TGG - chr3.3:92893804-92893823 None:intergenic 50.0%
!! TGGAGGCGAGTTTGTTAGCA+TGG - chr3.3:92893807-92893826 None:intergenic 50.0%
ACAGTTGGAGGAGGAGAAGC+AGG - chr3.3:92894202-92894221 None:intergenic 55.0%
!! TGTTAGCATGGTGGACACGG+TGG - chr3.3:92893795-92893814 None:intergenic 55.0%
GCATAAGGCGGCTTCATCCG+CGG - chr3.3:92893761-92893780 None:intergenic 60.0%
GTCGCAGGTGGAGCATAAGG+CGG - chr3.3:92893773-92893792 None:intergenic 60.0%
GTGGTCGCAGGTGGAGCATA+AGG - chr3.3:92893776-92893795 None:intergenic 60.0%
! GGTGGTTGAGAGAGAAGCGC+CGG - chr3.3:92893834-92893853 None:intergenic 60.0%
!! AGAGAAGCGCCGGTGTTTGG+AGG - chr3.3:92893824-92893843 None:intergenic 60.0%
!! GAGAGAGAAGCGCCGGTGTT+TGG - chr3.3:92893827-92893846 None:intergenic 60.0%
!! ATGGTGGACACGGTGGTCGC+AGG - chr3.3:92893788-92893807 None:intergenic 65.0%
GTGGACACGGTGGTCGCAGG+TGG - chr3.3:92893785-92893804 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 92893706 92894585 92893706 ID=MS.gene69436
chr3.3 mRNA 92893706 92894585 92893706 ID=MS.gene69436.t1;Parent=MS.gene69436
chr3.3 exon 92893706 92893897 92893706 ID=MS.gene69436.t1.exon1;Parent=MS.gene69436.t1
chr3.3 CDS 92893706 92893897 92893706 ID=cds.MS.gene69436.t1;Parent=MS.gene69436.t1
chr3.3 exon 92894064 92894585 92894064 ID=MS.gene69436.t1.exon2;Parent=MS.gene69436.t1
chr3.3 CDS 92894064 92894585 92894064 ID=cds.MS.gene69436.t1;Parent=MS.gene69436.t1
Gene Sequence

>MS.gene69436

ATGAAGATCCAGTGCGACATCTGTAACAAACGGCAGGCTTCTTTATTCTGCACCGCGGATGAAGCCGCCTTATGCTCCACCTGCGACCACCGTGTCCACCATGCTAACAAACTCGCCTCCAAACACCGGCGCTTCTCTCTCAACCACCCTAATAATTCTCCCAACCATTTCCCTCTCTCTGATATTTGCCTCGTAATTCTCTCCAATATTCATTCTTTTATGTATACTATACTTGTTATATATAGAAAGAGACTAGCTAGTAATTATATATACTATTAATTTCATTTGATTATATCAACTTATGATTCATGAATTAACATATAATATTGATACATTGAGTTTGTTTCTATGTTTGTAGGAAAGAAGAGGTTTTGTTTTTTGTCAGGAAGATAGAGCGATTGTGTGCAAAGAGTGTGATTTGAAGATTCATGGTGTCAACGAACACACCAAGAAGCATAATAGGTTTCTTCTAAGTGGGATCAAGCTGCACTCTCCTGCTTCTCCTCCTCCAACTGTTCATGAAGAAACTCCTAGTTTTACTATATCTGAGTACTTGATAAACACTATCCCAGGTTGGAAGTTTGAAGATTTCCTTGACTCCCCTTCTTCTGTTCCCTCTCATGAACTACAACAGCAGAATCATATTGGTAAAGGTACTACATTGTCAACAGTGCATGATGATGTTAATTATCATATTCATGAGGAAAACATTCTGGTTTCTTTCTCTAGCGAGAGCATGTGTGGGATCTGTGTTCCTCAAGCACCACTGTATTATTCTGAAAAAATGGATAACAAATCAAACACAATTAATGAAGCCACCAATTTCAGCAGCAGTTTGGGGGATTTCACAGTTCCACAGATAACAATCCCTCCCAATTAA

Protein sequence

>MS.gene69436.t1

MKIQCDICNKRQASLFCTADEAALCSTCDHRVHHANKLASKHRRFSLNHPNNSPNHFPLSDICLERRGFVFCQEDRAIVCKECDLKIHGVNEHTKKHNRFLLSGIKLHSPASPPPTVHEETPSFTISEYLINTIPGWKFEDFLDSPSSVPSHELQQQNHIGKGTTLSTVHDDVNYHIHEENILVSFSSESMCGICVPQAPLYYSEKMDNKSNTINEATNFSSSLGDFTVPQITIPPN